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This study aims to identify the gully erosion typology and development using a geomorphological approach. Gully geomorphology features were executed using combined photogrammetric approaches: aerial photography (unmanned aerial vehicle, UAV) and terrestrial photo data (structure from motion, SfM). The UAV data are used to identify the gully orientation, while SfM derives the geomorphological features in the gully dimensions. Five canopy-free gully erosion points were selected for the UAV-SfM data acquisition. Typically, SfM data offer higher resolution (0.11–0.57 cm) than UAV data (0.61–2.08 cm). Modelling using SfM can provide an in-depth illustration of gully dimensions such as rill erosion, scars, and cracks. The findings demonstrate that the gully depth and width are larger on the middle slope. This phenomenon is influenced by the strength of the flow and the silt transported by the water, which reaches a peak on the middle slope. The lower slopes have a solid form since the power of the flow weakens as it transports the accumulated silt from the upper and middle slopes. The study’s findings can be relied on to guide communities in strengthening the gully body in the middle slope. Furthermore, the findings can be tested and adopted globally with comparable typologies.

Forest logging activities negatively affect various soil properties. In this study, we focus on the logging effects on soil water retention and associated pore size distribution. We measured the soil-water characteristic curves (SWCCs) on 21 undisturbed samples from three research plots: a reference area, a clear-cut area and a forest track. A total of 12 SWCC points between saturation and wilting point were determined for each sample with a sand box and pressure plate apparatus. The trimodal behaviour is highlighted by the dependence between soil moisture and suction. Therefore, we proposed a revised model by combining two exponential expressions with the van Genuchten model. The exponential terms describe the influence of macro-and-structural porosities, and the latter is used to calculate textural porosity. This new model with eight independent parameters was suitable to fit trimodal SWCCs in all samples. Results revealed that logging had the most destructive effect on large pores, and the soil on the forest track was the most affected. Both soil-air and available water capacity were reduced and the permanent wilting point increased as a result of damage to the soil structure and pore system. Observed increased organic carbon content in compacted soils can be attributed to slowed decomposition due to reduced air capacity and increased waterlogging susceptibility of damaged soils.

In Europe, the weather patterns require harvested grain crops to be dried before storage to prevent significant quality loss. The uneven movement of grains inside the drying equipment is a key issue affecting the drying process, causing under- or over drying the harvested crops and thus leading to quality degradation and ultimately to financial losses. To characterise the unevenness of material flow, we introduced a dimensionless displacement ratio. This dimensionless parameter was suitable for comparing the uniformity of the material movement processes within the dryer. Using experimental investigations and numerical simulations, we determined the effect of the lamella inclination angle, the friction between the grain-wall and grain-grain on the uniformity of the flow. The linear functions approximating the quantitative relationships were determined in all the cases. Our findings indicate a significant variation in the displacement ratio ξ corresponding to different lamella inclination angles and friction values demonstrating that the discrete element modelling approach provides further opportunities for determining the optimal operating parameters of mixed flow dryers.

Corrigendum in: J. For. Sci. 2026 Feb 27;72(2):106. doi: 10.17221/16/2026-JFS

The response of forest trees to bark beetle attack involves substantial changes in terpene content, which varies between resistant and non-resistant species. Terpenes serve as crucial chemical defences against pests, and their production can be influenced by prior attack history, water stress, and biotic interactions. Moreover, the effectiveness of terpenes in resisting bark beetle attack is influenced by their chemical composition. Different tree species produce different types and amounts of terpenes that can affect their overall resistance levels. In conifers, acyclic and cyclic monoterpenes and sesquiterpenes were significantly present in all samples. Acyclic monoterpenes, ketones, aldehydes, monocarboxylic acids and their esters, and aromatic and cyclic compounds have been identified in beech. A statistically significant decrease of compounds in infested trees was determined in pine (cis-β-ocimene, neo-allo-ocimene, terpinene-4-ol, and δ-cadinene), and fir (acetophenone, benzonitrile, phenol, and zonarene). In addition, increased benzaldehyde production was observed. However, in infested beech trees, only increased production of some aliphatic and aromatic compounds (2-butanone and 3,5-octadien-2-one, 2-methyl-4-pentenal and 2,4-hexadienal, octanoic acid, nonanoic acid, 3,4-dimethyl-2,5-furandione, acetophenone, benzeneacetaldehyde, 2-ethyl-1H-pyrrole, β-ionone-5,6-epoxide, β-cyclocitral, and geranyl acetone) was found. We investigated the changes in the terpene composition of surviving trees in bark beetle-infested stands of beech (Fagus sylvatica), pine (Pinus sylvestris), and fir (Abies alba). Our data showed that the distribution of different groups of volatile compounds varied according to the tree species.

Forest headwater streams serve as critical interfaces between terrestrial forests and downstream aquatic ecosystems, playing essential roles in the storage and movement of carbon (C) and nutrients. However, despite their importance, our understanding of the dynamics of plant litter calcium (Ca) and magnesium (Mg) stocks within these streams remains limited. In this study, we conducted a quantitative analysis of the spatiotemporal dynamics of plant litter Ca and Mg concentrations and stocks in a subtropical forest headwater stream from March 2021 to February 2022. We found that: (1) the average concentrations of litter Ca and Mg were 9.9 and 0.7 mg/g, respectively, with mean stocks of 8 792.3 and 620.8 mg/m², respectively; (2) significant variations in litter Ca and Mg concentrations were observed among non-woody debris (13.1 and 0.9 mg/g), fine woody debris (9.0 and 0.5 mg/g), and coarse woody debris (6.1 and 0.4 mg/g), though plant litter type did not significantly affect the stocks of Ca and Mg; and (3) the stocks of Ca and Mg were positively correlated with factors such as rainfall amount, rainfall frequency, water temperature, flow velocity, water depth, electrical conductivity, and discharge, while negatively correlated with stream water alkalinity and dissolved oxygen levels. These findings highlight the critical role of plant litter in headwater streams as a component of forest nutrient stocks and provide empirical support for incorporating headwater streams into the assessment of nutrient stocks and fluxes in forest ecosystems.

The forest dynamics of the Ore Mountains (Krušné hory), Czech Republic, reveal a historical decline of natural mixed forests, especially those dominated by the Hercynian mixture and European beech (Fagus sylvatica L.), due to the expansion of metallurgy and glassmaking in the 15^th century. This led to large-scale reforestation with Norway spruce [Picea abies (L.) Karst.], resulting in single-layered monocultures. Although these monocultures provided valuable timber, they proved highly susceptible to both biotic and abiotic stressors. Throughout the 20^th century, the stability of these forests further deteriorated due to air pollution (notably SO₂ emissions), the unsuitable selection of substitute species, and the proliferation of pathogens. The cumulative impact of these disturbances caused soil acidification, degradation, and weed encroachment, severely limiting the regenerative capacity of forest ecosystems in this region. This article presents model examples of species composition shifts, spatial structure changes, and evolving management practices in the Ore Mountains. It discusses strategies for establishing diverse and resilient stands that align with long-term forest planning goals. These approaches aim to maintain both productive and ecological functions of forests under changing environmental conditions while minimising restoration costs. Importantly, forest management and conversion strategies must also account for economic optimisation, ensuring that ecological goals are met in a financially viable manner. The strategies and case studies presented here offer promising, albeit preliminary, directions for future forest management. Their broader application will require further refinement and long-term experimental validation to ensure sustainability in both ecological and economic terms.

The brown rot of apple and stone fruits caused by Monilinia fructigena is a widespread disease causing serious losses in fruit production. The most common way the pathogen spreads is via airborne conidia. Therefore, air samplers can effectively monitor its occurrence. In this study, we have conducted a comparative sampling of two cheap air samplers – rotorod spore traps called ROTTRAP 52 and AMETRAP. An optimised quantitative real-time PCR assay with a hydrolysis probe evaluated samples. 14 concurrent samplings were positive in all cases, showing higher spore counts in almost all AMETRAP samples obtained under various weather conditions. The daily maximum air temperature was the only significant meteorological variable positively affecting the recorded spore counts. Both rotorod samplers are an efficient and economic option for horticulturists and researchers for M. fructigena air inoculum monitoring.

The resilience of lactic acid bacteria (LAB) in commercial probiotic products remains a critical area of investigation, particularly regarding their capacity to survive the harsh gastric environment. Scientific guidelines indicate that at least 6 log CFU·g–1 of viable probiotics must reach the intestines to achieve therapeutic benefits, which often requires an initial concentration of 8–9 log CFU·g–1 in the product. However, national regulations may specify lower thresholds; for example, Turkish Food Legislation requires 6 log CFU·g–1 for probiotic products and 7 log CFU·g–1 for kefir products. This study evaluates the in vitro gastric survival of LAB in 20 probiotic-labelled foods and 5 supplements available in the Turkish market using a simulated gastric model. Results reveal that 75% of the marketed probiotic-labelled foods comply with their label claims. Additionally, 55% of the samples demonstrate LAB strains fully resistant to gastric acidity. Dairy-based products exhibit significantly better survival rates under simulated gastric conditions compared to supplements, highlighting their potential for enhanced therapeutic efficacy.

The productivity and quality of the entire flock are negatively impacted by heat stress in chickens, which can have major repercussions, particularly in crowded farming settings where diseases are easy to spread and hard to control. This study uses deep networks and optical flow to identify heat stress in chickens. The technique focuses on identifying obvious signs of heat stress, such as panting and open-mouth breathing in chickens. There are two phases to the suggested approach: (1) using a deep network to detect open-mouth breathing in chickens; (2) using the Gunnar Farnebäck algorithm to compute the optical flow vectors of the wattle, the breathing frequency is estimated in the Fourier domain for the detection of panting chickens. The proposed method was tested on the obtained dataset and demonstrated its ability to recognise heat-stressed chickens in crowded conditions, achieving an overall performance metric of 0.90 by integrating the results of both phases. The two-phase approach, which incorporates the open-mouth breathing behaviour and panting frequency, improves the efficiency and assures robust, reliable heat stress detection.

Effective weed management is crucial in the critical period of sugar beet production, but often lacks sustainability and environmental protection. Recent advancements in sensor-based weed control systems have rendered the latter a realistic prospect, which demands detailed analyses, especially under suboptimal field conditions. The present study analysed six robotic-assisted weed control systems (RAWS) in three experiments on sugar beets in 2024, conducted under dry soil and high weed pressure. The experiments included sensor-based inter-row and intra-row hoeing, spot- and band-spraying and were compared to a broadcast herbicide treatment and an untreated control. Weed control efficacy (WCE) in the intra- and inter-row areas, as well as weed species composition and crop plant damage, were assessed after treatment. The data show that intra-row WCE of two hoeing robots (Farming GT® and Robovator®) equipped with selective intra-row blades achieved up to 80%, which was higher than the broadcast herbicide control with 67% WCE. In the inter-row area, Farming GT® robotic hoeing and ARA® spot-spraying resulted in more than 90% WCE, which was equal to the broadcast herbicide application. Weed species composition was not affected by the different RAWS. Crop plants were affected by all hoeing treatments with maximum non-lethal burial rates of 33%. The highest lethal uprooting of crop plants occurred after Farming GT® robotic hoeing, at 5.5% overall. The results demonstrate the great potential of robotic weeding to replace broadcast herbicide applications.

Corrigendum in: Czech J. Anim. Sci. 2024 May 31;69(5):209. doi: 10.17221/83/2024-CJAS

This study was conducted to evaluate the protective impacts of green tea extract (GT) against cypermethrin-induced (CYP) neurotoxicity parameters such as acetylcholinesterase activity, oxidative stress, immune response, histopathology, apoptosis, and DNA damage in the brain tissues of rainbow trout (Oncorhynchus mykiss). The trial was divided into four groups (group 1, 2, 3 and 4). Group 1 was the control, while group 2 was treated with 0.1 mg/l of CYP, group 3 was treated with 0.1 mg/l of CYP + 100 mg/l of GT and group 4 was treated with 0.1 mg/l of CYP + 200 mg/l of GT for 14 days. While the level of malondialdehyde increased, the exposure to CYP in group 2 resulted in a reduction in acetylcholinesterase, lysozyme, total immunoglobulin, white blood cell, superoxide dismutase, and catalase levels in the brain tissues, as compared to group 1. Furthermore, CYP exposure in group 2 resulted in severe hyperaemia in meningeal and parenchymal vessels, accompanied by significant degeneration and necrosis. In addition, neurons in group 2 exhibited pronounced cytoplasmic expressions of 8-OHdG and caspase-3. On the contrary, both doses of 100 mg/l and 200 mg/l of GT demonstrated remarkable neuroprotective impacts against CYP toxicity across all parameters as mentioned above. In summary, this study conclusively showed that the administration of 200 mg dose of GT yielded more pronounced neuroprotective impacts, surpassing the impacts observed with the 100 mg dose of GT. The higher dosage effectively reduced CYP-induced oxidative stress, apoptosis and DNA damage, while exhibiting an enhanced immune response.

Corporate social responsibility (CSR) is also implemented in agriculture, where larger agricultural holdings have a higher influence. We focused on them and situated our study to a country with a large average farm acreage – the Czech Republic. The aim of the paper is to assess to what extent large agricultural holdings perceive socially responsible behaviour as being important and how it is manifested in their relationship to the stakeholders and the behaviour in economic, social and environmental areas using Carroll’s pyramid and concept of 3 ‘P’. It is also examined if the higher perception of CSR influences the assets. Firms were grouped according to the perceived CSR by a cluster analysis. The highest economic responsibility in cluster 1 corresponded to the relatively high value of the assets, but the highest was in cluster 2 and 3 with important legal and ethical responsibilities. Cluster 4 had the lowest CSR, the same as the value of the assets, but further investigation is needed. The most important stakeholders were the owners and employees, but also the range of activities was provided for the locals. CSR and the importance of the stakeholders in large Czech agricultural holdings were highly perceived, especially on an economic level and in the social area.

Perennial ryegrass (Lolium perenne L.) has potential in the phytoremediation of cadmium (Cd)-contaminated soil due to its strong Cd accumulation capacity and high biomass. In this study, we investigated the growth physiology, Cd accumulation, and metabolites of L. perenne roots under different soil acid stress levels (pH 4.0, 4.5, 5.0 and 6.0) and Cd concentrations (100 and 0 mg/kg) after 90 days of growth. The results showed that soil acid stress significantly impacts the remediation capability and physiological metabolic properties of L. perenne. Based on root Cd content and enrichment coefficient, soil pH between 4.5 and 5.0 was more conducive to Cd accumulation. The growth physiology and Cd accumulation of L. perenne were inhibited under high soil acid stress (pH 4.0). High soil acid stress caused a decrease in root length, root volume, and root biomass of L. perenne. Root malondialdehyde (MDA) content and the activity of antioxidant enzymes (catalase (CAT) and peroxidase (POD)) increased significantly in response to high soil acid stress to enhance tolerance. Metabolomics analysis revealed that acid stress resulted in significant changes in certain metabolites. Tartaric acid, fructose and amino acids (glutamate and lysine) in the roots of L. perenne were compatible solutes under acid stress. This study indicated that L. perenne has strong physiological and metabolic tolerance, as well as Cd accumulation ability, in response to soil acid stress.

Rice cropped policy has been constantly changing, affecting the value of irrigated land. This study aims to examine distribution patterns of irrigated land value from a new perspective of uncertainties in single- and double-cropped rice policies in China. The real options approach was employed to calculate irrigated land value under policy uncertainty, and Moran’s index was applied to identify the spatial distribution characteristics of irrigated land value at the provincial level. The empirical results were as follows: significantly positive spatial correlations existed between the land value under two cropped rice policies. The northeast region of China had a low-value clustering effect based on geographical characteristics. In contrast, the southern provinces of China had a high-value clustering effect based on the criteria of administrative regions. This study considered policy uncertainty when evaluating the economic effect on irrigated land when revealing spatial correlation in land value. We provide a theoretical and empirical basis for the formulation of cropped rice policies.

When 2-chloro-6-(trichloromethyl) pyridine (nitrapyrin) is applied alone, it typically does not significantly increase crop yield. Therefore, we combined gamma-aminobutyric acid (GABA) with nitrapyrin to address the limitations of nitrapyrin in enhancing yield. We conducted indoor incubation experiments and pot experiments in Chernozem and Calcic Kastanozem, respectively. The results demonstrated that GABA exerted an influence on the effectiveness of nitrapyrin by altering its degradation rate. In Chernozem, GABA accelerated nitrapyrin degradation, whereas, in Calcic Kastanozem, the results were the opposite. The pot experiment results showed that the combination of nitrapyrin and GABA increased rice total biomass by 5%, grain yield by 18 ± 2%, and plant nitrogen (N) uptake by 9 ± 1% compared to nitrapyrin applied alone. The increase in yield was attributed to the combined effect of nitrapyrin and GABA, which elevated root biomass and leaf area. In contrast, the effect of GABA on yield through altering the degradation rate of nitrapyrin was weaker. Therefore, the combination of nitrapyrin and GABA combined with urea increases rice yields in Chernozem and Calcic Kastanozem. The aim of this endeavour was to foster the development of a novel fertiliser product that offers both favourable agronomic outcomes and environmental benefits.

Listeria monocytogenes is capable of forming biofilms on the food contact surfaces, increasing the risk of food contamination by this pathogen. The disinfectant benzalkonium chloride (BC) is commonly used to control L. monocytogenes in the food industry. This study aimed to investigate effects of BC on L. monocytogenes biofilms. Biofilm biomass was measured by the microplate method with crystal violet staining. Results from the broth microdilution method showed that the minimum inhibitory concentration (MIC) of BC against L. monocytogenes 10403S was 8 μg·mL^–1. Sub-MICs of BC inhibited the biofilm formation and lethal concentrations of BC removed mature biofilms of L. monocytogenes 10403S. The presence of BC reduced extracellular proteins and exopolysaccharides in biofilms. Additionally, upregulation of quorum sensing gene luxS and agrBDCA and downregulation of flagellum motility genes flaA, motA, and motB were observed in the presence of BC. The BC disinfectant has an excellent anti-biofilm activity against L. monocytogenes.

Pattern recognition receptors (PRRs) play multiple roles in plants. As a kind of PRRs, chitin elicitor receptor kinase 1 (CERK1) proteins were reported to function in plant resistance to fungal and bacterial pathogens, and tolerance to salt stress. In this study, a predicted cowpea CERK1 homologous gene, designated as VuCERK1, was identified by database search. VuCERK1 protein contains 618 amino acid residues, with a predicted molecular mass of 67.5 kDa and a predicted isoelectric point of 5.04. VuCERK1 shows 58% and 60% sequence identity with AtCERK1 and OsCERK1, respectively. VuCERK1 also shows similar subcellular pattern with AtCERK1 and OsCERK1, suggesting VuCERK1 may function in cowpea immune responses. Gene expression assay indicated, that VuCERK1 was expressed in four different seedling tissues tested, comprising first leave, epicotyl, hypocotyl and root, and it could be induced by salt stress. Furthermore, transient expression of VuCERK1 in Nicotiana benthamiana induced obvious cell death. In addition, heterologous overexpression of VuCERK1 in Arabidopsis thaliana conferred enhanced disease resistance to Pseudomonas syringae pv. tomato strain DC3000 hrcC^- (Pto DC3000 hrcC^-).

The breed can influence the results of haematological and biochemical blood tests, with sighthounds traditionally mentioned. It may also affect certain urinary parameters. This study aimed to compare urinary protein and creatinine concentrations and their ratio (UPC, urine protein to creatinine ratio) between sighthounds and non-sighthounds and to evaluate these parameters in various sighthound breeds. Urine samples from clinically healthy dogs were collected via normal voiding, representing both sighthound and non-sighthound breeds. The protein and creatinine concentrations in the urine samples were determined, and their ratio was subsequently calculated. A total of 191 urine samples from sighthounds and 90 urine samples from non-sighthound breeds used as a control group were evaluated in the study. In sighthounds, significantly lower urinary protein concentration (248.8 mg/l and 299.8 mg/l, respectively; P = 0.045) and significantly higher urinary creatinine concentration (23.0 mmol/l and 17.5 mmol/l, respectively; P = 0.000) and lower UPC values (0.13 and 0.18, respectively; P = 0.000) were observed in comparison to the entire control group. The UPC values were found to be significantly lower in Greyhounds and Spanish Greyhounds compared with non-sighthounds. Although statistically significant changes were identified, they are unlikely to be of great clinical importance.

The aim of this study was to determine the effects of elevated CO₂ (eCO₂) on the quality and cadmium (Cd) accumulation of vegetables grown in soil with a background Cd level or in Cd-contaminated soil. We used four types of vegetable: pak choi (Brassica rapa L.), water spinach (Ipomoea aquatica Forssk.), cherry radish (Raphanus raphanistrum subsp. sativus (L.) Domin) and pepper (Capsicum annuum L.). Cd stress significantly reduced vegetable vitamin C and reducing sugar content; however, under eCO₂, vitamin C and reducing sugar content levels were significantly higher than they were under ambient CO₂ (aCO₂) levels. The nitrate content of pak choi, cherry radish and pepper was reduced under eCO₂, and the effect was more pronounced among plants grown under Cd stress. The Cd content of cherry radish and pepper roots grown under eCO₂ and Cd stress was significantly reduced (29.2% and 18.5%, respectively) compared with plants grown under aCO₂ conditions and Cd stress. The Cd transfer coefficient of pak choi and water spinach grown under eCO₂ and Cd stress was significantly lower (22.7% and 25.2%, respectively) than under aCO₂ conditions. Our data suggest that growing vegetables under eCO₂ is beneficial, especially when grown in Cd-contaminated soil, because Cd accumulation is reduced and vegetable quality is improved.

The lowering of loads at gradual tree felling is the riskiest activity performed by tree climbers. During this activity, great forces emerge and act on the felled tree, while the tree climbers use the tree itself to anchor and secure their stance. This research aims to find out whether certain methods of work or the use of certain rigging for lowering loads exhibit positive features in reducing the forces acting on the anchoring point of the rigging system. The work methodology consists of three operations: (i) the calculation of the coefficient of shear friction for the combination of 4 ropes and 5 lowering devices – altogether 20 combinations; (ii) the mathematical modelling of the maximum forces acting on the lowering loads of known weight; and (iii) the verification of mathematical modelling using a series of measured experiments of lowering loads of known weight. The research results present the calculation of the shear friction coefficient for 20 combinations of rigging rope and lowering devices. The maximum magnitudes of the forces which may act on the upper anchoring point of the rigging system at the known load weight were additionally calculated. A total of 240 force values were measured when a load of 12 kg in weight falls according to a predetermined model situation. The results indicate that the greatest influence on decreasing the impact force generated by the falling load is that of the load attachment, reduced load weight, shortened length of the fall or elongation of the total rope length in the rigging system. The differences in the coefficients of shear friction are apparent in the results of measurements, too.

The aim of this work is to characterize the physicochemical and techno-functional properties of dried fig flour to investigate its effect on the qualities of biscuits on the one hand and to encourage the use of this aromatic and medicinal plant in the agri-food industry. During this study, figs and wheat grains were dried and subsequently ground to obtain a flour that was used in various proportions of 0%, 50% and 100%, respectively in the manufacture of the biscuit. The flours were examined for proximate composition, polyphenol and tannin levels, along with functional properties. Flour derived from dried figs showed higher levels of crude ash, sugar, fiber, polyphenol and tannin, as well as greater water absorption capacity and density. The results indicated that incorporating dried and rehydrated fruits increased the total antioxidant activity of the biscuits compared to the control sample. The detailed organoleptic analysis, conducted through a tasting test revealed that all tasters found the dry biscuits acceptable. Biscuits formulated with 50% fig powder exhibited improved sensory attributes, including attractive colour, a crispy texture, and a more pronounced flavour. The use of this aromatic and medicinal plant in the food industry, particularly in biscuit production, holds significant potential. It enhances the nutritional value while imparting a sweet taste, distinctive flavour and natural colour. Additionally, its use contributes to improving the functional attributes of biscuit products, offering a sustainable approach to valorizing dried fig flour.

This study investigates differences in carbon storage between managed and unmanaged forests in the growth conditions of Central Europe. Norway spruce (Picea abies), European beech (Fagus sylvatica) and pedunculate oak (Quercus robur) dominated forest types were considered, as these are the most common forest species in the Czech Republic. Scots pine (Pinus sylvestris), as the second most common species, was excluded due to lacking relevant reference data on unmanaged forests. Managed and unmanaged variants of each forest type were assessed in terms of carbon sequestered in biomass, dead wood and harvested wood products (in the managed variant). Harvested wood products yielded during two rotation periods were considered, including their substitution effect as well as respiratory losses, to fully assess their contribution to carbon balance. Average carbon storage in the above-ground biomass and deadwood was lower in the managed forest compared to the unmanaged forest in comparable growth conditions. However, this difference is in our model examples compensated by carbon stored in the harvested wood products including their substitution effect in the managed forests of Norway spruce and pedunculate oak. Contrarily, managed European beech forests showed, in our case, slightly lower carbon storage compared to the unmanaged alternative. The estimates for all species are considered to be rather conservative due to the assumed factors affecting the results. Due to generally limited comparative data on unmanaged forests in the region, the results should be interpreted with caution.

High-pressure processing (HPP) represents a promising alternative to conventional Holder pasteurisation (HoP) used by human milk banks worldwide. The objective of this study was to identify whether the HPP would achieve the same or better retention of the content of selected analytes than the HoP. Samples collected from 15 breast milk donors were processed in four ways: i) no treatment; ii) HoP; iii) HPP in cycles (350 MPa, 4 cycles); iv) continuous HPP (350 MPa, 20 min). The content of secretory immunoglobulin A (sIgA), lactoferrin and lysozyme was determined using commercially available ELISA kits, and the lipase activity was assessed using an A-lipase activity assay kit. Data were compared statistically using paired t-tests. HoP significantly reduced the content of lysozyme and lactoferrin as well as lipase activity (P < 0.001). Cycled HPP significantly decreased lipase activity (P = 0.002), while continuous HPP led to a significant decrease in lysozyme content (P = 0.001) and lipase activity (P = 0.014). Cycled HPP showed high retention of pretreatment levels of lysozyme – median 99 (88; 99%), lactoferrin – 84 (66; 105%), and sIgA content – 83 (28; 117%). Among the studied treatment regimens, the best preservation of initial levels of bioactive components was achieved using HPP at 350 MPa in cycles.

Congenital anomalies affecting the spinal column are frequently observed in certain dog breeds. One such condition occurs at the junction between the lower back and the sacrum, where vertebrae exhibit mixed structural characteristics. This retrospective study analysed imaging records of Rhodesian Ridgebacks collected over 28 years, selecting only those that met strict positioning standards for evaluation. A total of 2 012 cases were analysed using established classification methods, identifying three distinct structural variations: a typical junction, a bilaterally altered morphology, and an asymmetrical form affecting one side. The prevalence of these variations was 7.4%, with symmetrical alterations found in 5% of cases and asymmetrical alterations in 2.4%. Statistical analysis did not reveal a significant association between these anatomical variations and canine hip dysplasia (CHD) (P = 0.170 0). These findings support previous research indicating that there is no direct link between these structural variations and joint disorders in this breed. The study represents the most extensive investigation of its kind in this canine population, highlighting that such vertebral variations are frequently observed in routine radiographic screenings, but despite decades of research, consistent clinical correlations remain elusive – underscoring the need for further systematic investigation.

Emergence predictive models can facilitate weed management, but estimating cardinal temperatures for germination of target species is necessary. Germination tests at a range of alternating temperatures from 12.5/2.5 °C to 35/25 °C were conducted to estimate cardinal temperatures of Persicaria lapathifolia (L.) Delarbre, Polygonum aviculare L. and Solanum nigrum L. Two statistical methodologies were tested: the Thermal time-to-event model (TTEM) and the Threshold limit model (TL). Germination of P. aviculare was maximum at low-mid temperatures, where its optimal range probably lies, and decreased at high temperatures. No differences were observed between the base (T_b) values estimated for this species with the two models (TTEM 3.5 °C, TL 4.1 °C), while a significantly higher ceiling (T_c) value was determined with TTEM (TTEM 41.5 °C, TL 33.6 °C). The Germination of P. lapathifolia and S. nigrum increased monotonically with the rise in temperature, indicating that their optimal temperature lies above the highest tested temperature. TTEM could not be applied to these species since it requires data from the supra-optimal thermal range. TL models could instead estimate T_b values (9.4 °C and 15.4 °C for P. lapathifolia and S. nigrum), while the lack of data in the supra-optimal thermal range impeded the estimation of T_c. actual and predicted PFAs affecting concurrently all species were caused by the thermal conditions of the year.

The sustainable management of European forest ecosystems necessitates innovative mechanisation solutions to address operational challenges in hilly, mountainous, and ecologically sensitive terrains. Hybrid-drive unmanned vehicles (HDUVs) present a transformative potential by integrating fuel-electric powertrains with autonomous navigation systems, enabling energy-efficient operations with minimal environmental impact. This review synthesises and critically analyses advancements in three critical domains: (i) dynamic modelling and chassis design for enhanced terrain adaptability, (ii) hybrid powertrain optimisation for reduced emissions and extended operational range, and (iii) the integration of unmanned systems for precision forestry tasks. By examining multi-body dynamics, power management strategies, and AI-driven navigation algorithms, we elucidate the role of HDUVs in improving operational efficiency while mitigating soil disturbance and carbon footprint. The review identifies prevailing research gaps and suggests that future work should prioritise the development of standardised testing protocols and foster cross-disciplinary collaboration to align HDUV development with EU biodiversity and climate objectives.

Innovation is the driver of sustainable business development and is essential to promote high quality econo-mic development in the country. Are more bank loans better for small and medium-sized enterprises (SMEs) innovation? Therefore, based on mixed cross-sectional data of county sweet potato processing enterprises, this study applied the econometric model to explore the impact of bank loans on county agrifood SMEs innovation. We find that there is an 'inverted U-shaped' relationship between bank loans and county agrifood SMEs innovation. The analysis of the mechanism shows that bank loans can not only alleviate the problem of innovation financing constraints for agrifood SMEs, but also provide financial support for the innovation activities of agrifood SMEs as a result of trade openness. This study has important practical implications for promoting county agrifood SMEs innovation in China and promoting high-quality county economic development.

Potato (Solanum tuberosum L.) is an economically significant food crop in China, and increasing tuber yield is a national priority. We conducted a meta-analysis utilising 180 studies and 1 583 pairs of observations to quantify the effects of fertilisation on potato tuber yield using data on climate, soil nutrients, and planting strategies. Compared with no fertilisation, fertilisation increased tuber yield by 33.64% overall. Applying single N, P, or K fertilisers increased yield by 33.64, 23.37, and 16.18%, respectively; combined NP, NK, and PK applications increased yield by 33.64, 36.34, and 19.12%, respectively. The greatest yield increase (49.18%) was achieved when NPK fertilisers were applied together. Average annual precipitation had the strongest effect on tuber yield, followed by cultivar identity and the availability of soil potassium. Under appropriate fertilisation regimes, tailoring planting strategies to local climate and soil nutrient status can maximise potato yield and improve economic returns. These findings have implications for future potato cultivation in China.

Cerebellar hypoplasia caused by feline panleukopenia virus (FPV) is well documented in domestic cats. Still, it remains unreported in wild felid species, including the Amur leopard cat (Prionailurus bengalensis euptilura). Understanding the impact of such viral diseases on wild populations is crucial for advancing conservation efforts and protecting wildlife. An orphaned Amur leopard cat exhibiting idiopathic ataxia was rescued. Initial diagnostics, including physical examination, radiography, and blood analysis, yielded no remarkable findings, though its clinical signs indicated an underlying neurological problem. Subsequent real-time polymerase chain reaction tests detected FPV. Magnetic resonance imaging (MRI) revealed brain lesions, including reduced cerebellar parenchyma and cerebrospinal fluid occupying the space where the cerebellum should be located. These findings suggested cerebellar hypoplasia caused by in utero FPV infection. The Amur leopard cat was euthanised owing to its permanent disability, and the necropsy confirmed a markedly shrunken cerebellum. At the same time, histopathology identified decreased cellularity of the molecular and granular layers of the cerebellar cortex. These results coincided with the MRI findings. This report suggests that cerebellar hypoplasia caused by FPV can occur in wild felid species.

Geographical indication (GI) represents the specific good quality and reputation of the regional characteristics of agricultural products, which is a positive approach for stabilising the export of agricultural products, realising high quality and high prices for agricultural products and rural revitalisation. Based on the China-EU Geographical Indications Agreement, this paper discusses the differences between China and the European Union (EU) in terms of GI protection from three aspects: the institutional framework, the operational system, and the operational status of GIs. The purpose of the study is to identify the differences between China and the EU in the protection of GIs, China's shortcomings, and gaps, and to propose policy recommendations for China to protect GI products better, exploit the economic impact of GIs and enhance the competitiveness of China's exports.