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Results 1291 to 1320 of 4551:

Effect of catalase on the growth performance, antioxidation, and microbial metabolism of weaned rabbitsOriginal Paper

Ning Liu, Yien Lin, Shuaibao Wang, Jianping Wang

Czech J. Anim. Sci., 2023, 68(12):508-515 | DOI: 10.17221/133/2023-CJAS

The present study aimed to investigate the effect of catalase (CAT) on the growth performance, antioxidation, and microbial metabolism of weaned rabbits. Dietary treatments consisted of control and CAT supplementation at 100 (T1), 150 (T2), and 200 IU/kg of diet (T3). A total of 240 weaned rabbits were randomly assigned to 4 groups with 6 replicates of 10 rabbits each. The feeding trial lasted for 28 days. Results showed that T2 and T3 increased (P < 0.05) body weight gain and gain/feed ratio, and decreased (P < 0.05) diarrhoea rate, compared to the control. Also, serum antioxidative parameters and oxidative stress products were beneficially regulated (P < 0.05) by the dietary CAT administration. Faecal microbiota including Bacteroidetes, Prevotella, and Bifidobacterium in T2 or T3 was increased (P < 0.05). Dietary CAT with changed microbiota further influenced the metabolites from carbohydrates and proteins, evidenced by increased lactic acid, acetate, branched-chain fatty acids, and short-chain fatty acids, and decreased valerate, isovalerate, methylamine, tryptamine, putrescine, cadaverine, spermidine, and total amines. It is concluded that CAT can be used to improve growth performance by beneficially regulating the antioxidation, microbiota, and metabolites in weaned rabbits.

The impact of the Russian import ban on EU agrarian exportsOriginal Paper

Lubo¹ Smutka, Josef Abrhám

Agric. Econ. - Czech, 2022, 68(2):39-49 | DOI: 10.17221/351/2021-AGRICECON

The main goal of the paper is to evaluate the impact of the Russian import ban on the development of agricultural exports from EU member states. The study is based on a time-series analysis of empirical statistical indicators. The analysed period is between 2009 and 2019. The source of data for individual analyses is UN Comtrade (2021). The evaluation of export performance is extended by hierarchical cluster analysis. The study quantifies the effects of the import ban on the EU and individual member states through two scenarios. Scenario 1 is based on the cumulative loss of the value of exports. Scenario 2 assumes a continuous development of the value of agricultural exports. Based on the results, it is possible to confirm that the application of the Russian food import ban had a significant impact on EU countries. The impact of sanctions varies across EU countries. Four specific clusters could be identified in the period under investigation. In the period after the ban, the distribution of individual countries among individual clusters changed significantly. The applied ban could be understood not only as an attempt at counter-sanctions. Import restrictions also aim to reduce Russia's dependence on food imports and promote national food security.

Organic fertilization induces changes in soil nitrogen mineralization and enzyme activitiesOriginal Paper

María Rosa Yagüe, Carmen Lobo, Pilar García

Plant Soil Environ., 2023, 69(1):38-43 | DOI: 10.17221/274/2022-PSE

In this study, we addressed the reuse of two organic waste products as fertilisers. To this end, soil fertilised with the spent mushroom substrate (SMS) or with an anaerobic digestate (DIG) was subjected to an incubation assay, and the results were compared with those from soil treated with a mineral fertiliser (MIN) and an unfertilised soil (CO). The soil was sampled after fertilisation and after 90 days of aerobic incubation. Nitrogen (N) mineralisation (NH4+ and NO3–) and oxidable carbon (OC) were determined. The impact of the treatments on the soil was evaluated by measuring the enzymatic activity of arylsulfatase (ARYL), ß-galactosidase (GAL), and urease (URE). The highest OC content was observed in the SMS treatment. After 90 days of incubation, the SMS treatment showed a lower mineral N content than the CO treatment. This finding was associated with N immobilisation. However, mineral N significantly increased ARYL activity in the DIG and MIN treatments, and URE activity was always higher at both sampling times in the SMS treatment. Initially, GAL activity was notably high in the DIG treatment but decreased after incubation, reaching similar values to those registered in the CO treatment. Organic fertilisation treatments induced different effects on soil N mineralisation, showing changes in the activity of the enzymes analysed.


Working capital management in the food and beverage industry: Evidence from listed European companiesOriginal Paper

Hakan Özkaya, ªebnem Yaºar

Agric. Econ. - Czech, 2023, 69(2):78-88 | DOI: 10.17221/383/2022-AGRICECON


Optimizing current assets and their financing resources is of great importance for firms to sustain their existence with the highest level of profitability. However, empirical evidence from the food and beverage industry on the effects of working capital management (WCM) on profitability is scarce and mixed. This study aims to understand how various components of WCM affect the profitability of listed European food and beverage (F&B) companies. For this purpose, static panel data methodology was used to test the relationship between profitability and WCM measured by the cash conversion cycle and its components. The results were checked for robustness by using dynamic panel data methodology. Our results indicate a negative relationship between profitability and the cash conversion cycle. Unlike previous studies that have analysed a single country, this study provides evidence based on analyses of the largest possible sample of listed European F&B companies.

Influence of the surface/volume ratio on the rheological properties of starch dispersionsOriginal Paper

Gerardo Lopez-Echevarria, Jorge Adalberto Huerta-Ruelas, Gonzalo Velazquez, Martin Jesús Nieto-Perez, Guadalupe Mendez-Montealvo

Czech J. Food Sci., 2023, 41(1):54-63 | DOI: 10.17221/155/2022-CJFS


This study aims to evaluate the influence of the surface/volume granule ratio of amaranth, corn, and potato starches on the rheological properties of pastes and gels obtained at 5 and 10% solids concentration through granulometry, scanning electron microscopy, rotational, and dynamic rheological measurements. The granule size distribution and the surface/volume ratio drove the rheological behaviour as a function of temperature and concentration. At a concentration of 10%, the consistency index of corn starch (CS) paste was 147.25 Pa·sn (n – flow behavior index), a higher value compared to pastes from potato starch (PS), 86.54 Pa·sn, and amaranth starch (AS), 44.48 Pa·sn. The lowest values of the loss angle tangent (Tan δ) in CS (0.052 and 0.035) at both solids concentrations suggested a better gel conformation. Noticeable changes in consistency index and storage modulus were observed in CS. A theoretical analysis of the surface/volume ratio change showed that CS reached a 2.2 value, much lower than the 4.5 and 5.8 values for PS and AS, respectively. These findings provide additional criteria for the food industry when choosing starches with suitable rheological behaviour as a function of surface/volume granule ratio and solids concentration.

Vital rates and their multidecadal trends in the fir-beech old-growth forest of Badínsky pralesOriginal Paper

Ladislav ©umichrast, Peter Jaloviar, Matú¹ Komendák, Samuel Targo¹, Stanislav Kucbel

J. For. Sci., 2023, 69(3):93-100 | DOI: 10.17221/167/2022-JFS


The study aimed to quantify annual mortality and recruitment rates on permanent research plots in the Badínsky prales old-growth forest. The data measured in four stands originate from six censuses, which together cover a 48-year period. The mean annual mortality rate reached 4.2% (DBH > 2 cm) or 2.3% (DBH > 8 cm). The mortality peaked in the first part of the observation period, probably indicating an intermediate disturbance activity, and the subsequent declining trend led to minimum annual mortality during the last 20 years. We found substantially higher fir mortality in comparison with beech, primarily during the first decades of the measurement period. Among three size categories, the stems with DBH 2–8 cm showed the highest mortality rate. We detected the minimum fir recruitment rate throughout the observation period; on the other hand, the beech recruitment rate was relatively high responding to the open canopy. Standing and lying deadwood volumes indicated similar trends like mortality did, and high values of fir standing deadwood observed during the first decades can signify that a relatively high proportion of mortality was related to the fir decline caused by air pollution.

Incidence of thermophilic, grass and rare arable weeds in cereal fields in the Czech and Slovak RepublicOriginal Paper

Michaela Koláøová, Ludìk Ty¹er, Theresa A. Reinhardt Piskáèková, Jana Májeková

Plant Soil Environ., 2023, 69(3):131-140 | DOI: 10.17221/439/2022-PSE

Our objectives were to characterise arable weeds of the Czech and Slovak Republic in cereals, especially considering important groups of species: thermophilic, weedy grasses, and rare and endangered species. The three-year phytocoenological survey was conducted from 2006 to 2008 in five climatic regions and eight different soil types. 379 relevés were recorded, and 281 weed species were found. The effects of both the climatic region and soil type were found to be statistically significant. Species richness increased with altitude. Several species were distinctly associated with soil type, from fertile chernozems to less fertile cambisols, while fluvisols were strongly associated with e.g. Calystegia sepium (L.) R. Br. The incidence of species outside their expected regions generally fit two categories: the most pervasive weeds found almost in all climate regions, such as Echinochloa crus-galli (L.) P. Beauv., or rare weeds in higher altitudes than expected, such as Lolium temulentum L. Rare and endangered species were more likely to be found in a colder climate and poorer soils, and this is linked to less intensive management; however, not all endangered arable species can tolerate such conditions. Even though this data is not recently collected, this is still valuable information on the distribution of weedy species, especially concerning the current interest in maintaining biodiversity.

Herbicide drift vs. crop resilience – the influence of micro-ratesOriginal Paper

Milan Brankov, Bruno Canella Vieira, Milo¹ Rajkoviæ, Milena Simiæ, Jelena Vukadinoviæ, Violeta Mandiæ, Vesna Dragièeviæ

Plant Soil Environ., 2023, 69(4):161-169 | DOI: 10.17221/58/2023-PSE

A greenhouse study was conducted to test the effects of low herbicide dose exposure on different crops measuring visible damages, plant height, leaf area, and dry matter. Seven crops were tested: lettuce (Lactuca sativa L.) cv. Novosadska majska maslena, oil pumpkin (Cucurbita maxima Duch) cv. Olivija, oilseed rape (Brassica napus L.) cv. NS Ras, pepper (Capsicum annuum L.) cv. Kurtovska kapija, soybean (Glycine max (L.) Merr) cv. ZP Laura, sunflower (Helianthus annuus L.) cv. NS Kruna, and tomato (Solanum lycopersicum L.) cv. Dunavski Rubin. Herbicide dicamba in the range of 0.14 to 1 155.6 g a.i. (active ingredient)/ha inhibited biomass, height, leaf area, and visual injury of all crops, while glyphosate doses from 0.48 to 3 840 g a.i./ha also reduced the growth of all tested species. A rate of 116 g a.i./ha mesotrione was needed to reach 80% visual injury in oilseed rape, while the same effects on lettuce only required 1.8 g a.i./ha of mesotrione. Tomato and oil pumpkin were also sensitive to low mesotrione doses, where only 1.3 g and 0.5 g a.i./ha of mesotrione was needed for 80% of biomass reduction, respectively. Lettuce was the most sensitive crop of all tested species; biomass was reduced by 80% by dicamba, glyphosate, mesotrione, and nicosulfuron at the low rates of 33 g a.i./ha, 19 g a.i./ha, 1.25 g a.i./ha, and 2.7 g a.i./ha, respectively. Among all herbicides, visible injuries were detected in dicamba at the lowest rates. Soybean was the most tolerant of glyphosate, mesotrione, and nicosulfuron. Based on the available literature and obtained results, herbicide off-target movement must be mitigated to maximise herbicide efficacy and decrease the negative influence on susceptible plants and the environment.

Network-based business model in the agri-food sector: A case study of Green FingersCase Study

Sagit Barel-Shaked

Agric. Econ. - Czech, 2023, 69(4):162-170 | DOI: 10.17221/2/2023-AGRICECON

Globalisation and reduced trade barriers have created a competitive business environment, requiring agri-food firms to adopt a new innovative perspective. This research study examines a network-based business model of a new venture offering services to avocado farmers and its potential impact on customer performance. A theoretical framework of a network-based business model in the agri-food sector was developed and implemented in the production of avocados. The Israeli agri-food firm, Green Fingers, was selected as the case study to analyse the value drivers of the network-based business model. The proposed business model can serve as a benchmark in evaluating other business models and developing practical strategies in the agri-food sector. The model is flexible, adaptable in meeting the evolving needs of customers and farmers, and applicable to other sectors. The model's building blocks emphasise the importance of networking and diffusing knowledge among key stakeholders, adapting to changing environments, maintaining environmental sustainability, collaborating with policymakers, and supporting farmers. The extended business model canvas may enhance the currently limited research on network-based business models in the agri-food sector from theoretical and empirical perspectives.

Flight activity of aphids in Serbia: investigation by water traps placed in sugar beet fieldsOriginal Paper

Olivera Petroviæ-Obradoviæ, ®ivko Æurèiæ, ®eljko Milovac, Andja Radonjiæ

Plant Protect. Sci., 2023, 59(2):185-192 | DOI: 10.17221/130/2022-PPS


Plant viruses, and aphids as their vectors, are limiting factors in sugar beet production. Viral plant diseases are currently impossible to treat but knowing the flight patterns of aphids can help in reducing the number of potential virus vectors. Monitoring of aphid flight activities in sugar beet fields was done using yellow water traps from April to the end of November. During the two years of investigation, a total of 5514 specimens from 75 different taxa were collected. The highest number of individuals was recorded at the end of May/beginning of June at all localities. This is the period when sugar beet develops intensively, so the risk of virus infection is the highest. The most numerous species were Aphis fabae Scopoli, Aphis spiraecola Patch., Phyllaphis fagi (L.), Myzus persicae (Sulzer), Rhopalosiphum padi (L.), Sitobion avenae (Fabr.), Acyrthosiphon pisum (Haris) and Therioaphis trifolii (Monell). The most important vectors are A. fabae, A. spiraecola and M. persicae. Aphis fabae is a species that feeds on sugar beet and causes significant damage both by feeding and by its vector activity. Myzus persicae was the most abundant in autumn, A. spiraecola was present throughout the whole flight-monitoring period. Among the caught aphids, twelve species alien to Europe were collected.

What factors contribute to the volatility of food prices? New global evidenceOriginal Paper

Mourad Zmami, Ousama Ben-Salha

Agric. Econ. - Czech, 2023, 69(5):171-184 | DOI: 10.17221/99/2023-AGRICECON


The adverse economic and social effects of the recent increases in food prices have prompted policymakers and academics to reconsider the potential causes of such increases. This paper contributes to the existing literature by investigating the causal effects of oil prices, fertiliser prices, global economic activity, and geopolitical risk on international food price volatility between January 1993 and December 2021. The research considers the aggregate food price index and the prices of various specific foods, including cereal, vegetable oils, dairy, meat, and sugar. The Glosten, Jagannathan, and Runkle-generalised autoregressive conditional heteroskedasticity (1,1) [GJR-GARCH(1,1)] model is employed to estimate the food price volatility series, while the causality-in-quantiles test is conducted to identify the drivers of food price volatility for different volatility regimes. The analysis suggests heterogeneous results regarding the significance of causal linkages. More specifically, the aggregate food price volatility is affected by oil prices, global economic activity, and geopolitical risk under different market conditions. The causality analysis also indicates that the volatility of cereal prices is the most sensitive to the four considered variables. Likewise, geopolitical risk is the most critical factor affecting all food commodities during almost all market conditions, while oil prices and global economic activity have limited predictive power. Finally, there is strong evidence that most causal linkages are confirmed during normal market conditions. Policy recommendations are subsequently derived.

Inheritance and allelism of brown midrib trait introgressed in agronomically promising backgrounds in pearl millet (Pennisetum glaucum (L.) R. Br.)Original Paper

Shashi Kumar Gupta*, Ponnaiah Govintharaj

Czech J. Genet. Plant Breed., 2023, 59(3):176-187 | DOI: 10.17221/93/2022-CJGPB

The lignin content of forage crops significantly affects the livestock’s forage intake and its dry matter digestibility. The brown midrib (bmr) trait is known for its association with a reduced lignin content and increased digestibility. Pearl millet inbreds having the bmr trait were derived from two populations ICMV bmr and WRajPop bmr via continued selfing up to 6–8 generations. The results from the crosses revealed the presence of the same single recessive gene that controlled the bmr in both the populations. The stable expression of the bmr gene in the new genetic background indicated that there was no effect of modifier genes. Four inbreds were identified with the stable expression of bmr (three in ICMV 155 and one in the WRajPop background) with a high biomass potential, which can serve as a bmr source in future pearl millet forage breeding programmes.

Changes in the intensity and impact of factors influencing consumer behaviour in the food market over timeOriginal Paper

Irena Anto¹ová, Jana Stávková

Agric. Econ. - Czech, 2023, 69(9):358-365 | DOI: 10.17221/191/2023-AGRICECON


The paper aims to identify the changes and intensity of the factors influencing consumers’ purchasing decision-making process in the food market. The research offers a unique comparison of the situation between the years 2007 and 2022 and reveals the emerging factors affecting consumer decisions. The authors conducted a representative questionnaire survey (N = 686) to determine the influence of individual factors. The data were processed using factor analysis, which allowed for the reduction of the number of factors (20) to four determinants covering 61% of the total variability in the dataset. The determinants of consumer purchasing decisions include traditional promotional elements, product parameters, newly formed factors based on sustainability and health awareness trends, and price discounts. Consumer behaviour appears to be influenced by a wide range of short- and long-term factors, and it is necessary to look at it comprehensively over a longer time horizon, taking current trends into account.

Pyrolysis temperature had effects on the physicochemical properties of biocharOriginal Paper

Xue Li, Hang Liu, Ning Liu, Zhentao Sun, Shifeng Fu, Xiumei Zhan, Jinfeng Yang, Rongxin Zhou, Hongda Zhang, Jiming Zhang, Xiaori Han

Plant Soil Environ., 2023, 69(8):363-373 | DOI: 10.17221/444/2022-PSE

Biochar is the carbon-rich product obtained when biomass is anaerobically heated. In this study, different materials (corn straw and peanut shell) and pyrolysis temperatures (350, 450 and 550 °C) affect the elemental composition, surface structure, and biochar properties. The results showed that the carbon (C) content in biochar increased as the temperature increased, but hydrogen (H) and nitrogen (N) did not change. The alkane overpressure of corn straw and peanut shell increased first and then decreased with the increased temperature; the fatty alkyl chain disappeared, but the ash content increased at 550 °C. At high temperatures, the aromaticity (H/C ratio) and hydrophobicity (O/C ratio) of biochar become "carbon-rich particles", while the polarity (O + N)/C decreases significantly. The pore wall of biochar became thinner with the increase in pyrolysis temperature, the internal pore structure became larger, and a large number of micropores appeared in biochar. Biochar pyrolysed at 550 °C has much higher C, ash content, pore, and stronger buffering capacity, and thus is more promising to improve soil health.

First report of Thanasimus femoralis (Zetterstedt, 1828) (Coleoptera: Cleridae) in forest nursery in the Western Black Sea Region of TürkiyeShort Communication

Nuray Öztürk, Beºir Yüksel

J. For. Sci., 2023, 69(8):360-365 | DOI: 10.17221/50/2023-JFS

In this study, the first observation of Thanasimus femoralis was conducted in Türkiye. Adults of T. femoralis and Thanasimus formicarius were collected from pheromone traps together with Ips sexdentatus, Ips acuminatus, Ips mannsfeldi and Scolytus intricatus in forest nurseries located in the Western Black Sea Region, between 2021 and 2022. The clerid and bark beetles were morphologically described. The aedeagus and antennae of T. femoralis were observed by scanning electron microscopy.

Progression of wheat Tan spot under different bioclimatic stages and agricultural practicesOriginal Paper

Salma Tissaoui, Noura Omri-Benyoussef, Hatem Chaar, Marwa Hassine, Jean-stephane Venisse, Bouzid Nasraoui, Amira Mougou-Hamdane

Plant Protect. Sci., 2023, 59(3):233-244 | DOI: 10.17221/103/2022-PPS

Tan spot, caused by Pyrenophora tritici-repentis, is a severe worldwide wheat foliar disease and has become common in Tunisia. Two hundred wheat field surveys were conducted in northern Tunisia during the 2017–2019 seasons to determine disease intensity and the correlation of the tan spot with agricultural practices and environmental factors. The disease infested 67% of the visited regions. The highest prevalence (87.5%), incidence (> 30%), and severity (> 10%) were observed in sub-humid regions, contrary to the middle semi-arid regions. Logistic regression was used to analyse the correlation of incidence and severity with various factors as independent variables. In a multiple variable model, the bioclimatic stages, variety, previous crop, humidity, and rainfall were significantly associated with tan spot incidence and severity. High incidence and severity were significantly correlated to the sub-humid regions, varieties Maâli, Razzak, Karim, and Carioca, rainfall, high level of humidity, and monoculture. The correlation between the tan spot and several environmental factors must be considered to ensure successful and sustainable disease management strategies.

Simulating differences between forwarding short and normal-length timberOriginal Paper

Simon Berg

J. For. Sci., 2023, 69(11):485-496 | DOI: 10.17221/41/2023-JFS

Normal log lengths in Norway are 3–6 m (NL), but occasionally there is a demand for short timber with a 2.5 m log length (ST). There are concerns that ST could reduce the forwarders' productivity. Six type stands were created based on harvester data. Different assortment distributions, conditions, and forwarders were simulated in each type stand. It was found that an additional ST assortment almost always decreased productivity (from –15.5 to +4%). Increased forwarding distance (m), more difficult driving conditions, and increased log concentration [m3·(100 m strip road)–1] decreased the productivity difference between sites with ST and NL and sites with only NL. Increased forwarder size increased the productivity difference between sites with ST and NL and sites with only NL. It is possible to load two stacks of ST on some forwarders. Such loading was more productive than loading one stack on longer forwarding distances, while the opposite was the case on short distances. However, loading two stacks of ST can lead to overloading.

Stand structure of a juvenile Paulownia Shan Tong plantation grown in the temperate dry climate zone in Hungary – A case studyShort Communication

Fruzsina Szabó, Károly Rédei, Tamás Ábri, Elza Kovács, Lajos Juhász

J. For. Sci., 2023, 69(12):550-556 | DOI: 10.17221/90/2023-JFS

In this paper, empirical relationships between diameter at breast height (DBH), crown diameter (CD), and stem number per hectare (N) were improved in a Paulownia Shan Tong plantation. Various functions of these variables were defined, focusing on growing space (Gs) and stem number per hectare. The linear crown index between CD and DBH seems to be particularly important in predicting stem number per hectare. Based on an analysis of the relationships, a CD-DBH (R2 = 0.7254) and a DBH-N graphic model (R2 = 0.7302) can be applied widely in plantation forestry. Under suitable site conditions, the investigated Paulownia hybrid can provide a higher increment in DBH than most of poplar hybrids at a certain age. The aim of this study was to explore the relationships between the Gs based on CD and DBH. These types of investigations are of fundamental importance in terms of the growing technology of tree plantations.

Agricultural commodity markets and the Turn of the month effectOriginal Paper

Peter Árendá¹, Jana Kotlebová

Agric. Econ. - Czech, 2023, 69(3):101-108 | DOI: 10.17221/17/2023-AGRICECON


The Turn of the month (ToM) effect is a calendar anomaly when the majority of returns of an asset are concentrated into several days around the end of the old month and the start of the new one. Until now, the investigation of the ToM effect has mainly been focused on the stock markets. However, this paper investigates the presence of the ToM effect in eight key agricultural commodity markets (cocoa, coffee, corn, cotton, rice, soybean, sugar, wheat), using three different alternatives of the ToM window, during the 2001–2021 time period. The results show a statistically significant ToM effect in the rice, coffee, and sugar markets. Further results show that the ToM pattern changed during the COVID-19 pandemic, and that, in the case of commodities with a statistically significant ToM effect, the ToM effect can be efficiently used to beat the buy & hold investment strategy convincingly.


Positive effect of fir-rowan intimate mixture on new forest floor and topsoil following afforestationOriginal Paper

Du¹an Kacálek, Jan Barto¹, Ondøej ©pulák

J. For. Sci., 2023, 69(2):80-92 | DOI: 10.17221/180/2022-JFS


Litterfall of silver fir contributes to development of forest floor similarly like Norway spruce litterfall does. The nutrient return to soil can be intensified by mixing these conifers with other tree species whose effects on soil are positive; our study deals with European rowan. The study aimed at firs and rowans in alternating rows (Fi-Ro) compared to the monospecific plantation of fir (Fi) at two former meadow sites with the stands of 20 and 17 years of age, respectively. Both organic forest floor and its mineral subsurface were sampled. Rowan admixed to the silver fir plantation improved both the uppermost layer and the topsoil as higher pH, more favourable soil-sorption properties and higher plant available magnesium were found below Fi-Ro compared to Fi. Fine dry matter was higher below Fi, which was reflected in higher organic carbon (Cox), combustible matter and nitrogen pools. Besides the effects of trees on the soil, silvicultural issues such as renewal costs and tree species performance in monospecific and mixed stands were presented and discussed.

Presence of zoonotic black-pigmented periodontal pathogens in the oral microbiota of pet and stray catsOriginal Paper

V Ozavci, HTY Dolgun, S Kirkan

Vet Med - Czech, 2023, 68(2):62-68 | DOI: 10.17221/59/2022-VETMED

Black-pigmented bacteria are one of the neglected species to cause periodontal disease in cats, and they are also zoonotic agents that pose an infection risk to humans. In this study, we aimed to determine the presence of Porphyromonas gingivalis, Porphyromonas gulae and Prevotella nigrescens in the oral microbiota of pet and stray cats. Dental swab samples were taken from 25 pet cats and 25 stray cats with symptoms of periodontal disease and then investigated by multiplex polymerase chain reaction using 16S rRNA species-specific primers. As a result of the multiplex PCR analysis, P. gingivalis 3/25 (12%), P. nigrescens 1/25 (4%), P. gingivalis + P. gulae 7/25 (28%), P. gingivalis + P. nigrescens 1/25 (4%), P. gulae + P. nigrescens 1/25 (4%), and P. gingivalis + P. gulae + P. nigrescens 2/25 (8%) were molecularly typed in the pet cats. In addition, 1/25 (4%) of P. gulae and 21/25 (84%) of P. gingivalis + P. gulae were typed in the stray cats. In 10/25 (40%) pet and 3/25 (12%) stray cat samples, no bacteria were detected by molecular typing. In summary, the results provide strong evidence that black-pigmented zoonotic pathogens are associated with cat periodontal disease.

Delineation of inbred lines of Indian mustard into diverse gene pools based on agro-morphological traitsOriginal Paper

Kunwar Harendra Singh, Guman Singh, Lal Singh, Nashra Aftab, Ajay K. Thakur

Czech J. Genet. Plant Breed., 2023, 59(2):109-116 | DOI: 10.17221/75/2022-CJGPB

The present study was conducted to explore the variability generated through recombination breeding for nine economically important traits in Indian mustard (Brassica juncea L. Czern & Coss.). Delineation of the inbred lines to different gene pools, based on genetic diversity, enables their utilisation in hybrid breeding. The unweighted pair group method with arithmetic mean (UPGMA) and a Euclidean distance matrix was used to delineate the inbred lines to clusters. The variability was studied using the range and coefficients of variation of the traits. Significant variability was observed for all studied traits except for oil content and days to maturity. 128 Indian mustard genotypes were grouped into four distinct gene pools based on genetic diversity. A set of 20 most diverse genotype combinations was produced. Promising inbred lines were identified and recommended as donors for the respective trait. The pedigree analysis of the inbred line groups revealed, that recombination breeding caused a large diversity as confirmed by the assignment of inbred lines with the same parentage to specific clusters.

Studies on the growth, immunomodulation and gut morphometry of Labeo rohita fed pectinOriginal Paper

Humaira Sehrish Hashmi, Noor Khan, Khalid Javed Iqbal, Mahroze Fatima, Khalid Mahmood Anjum, Sumaira Abbas, Muhammad Awais, Sadia Nazir, Muhammad Asghar, Mahanama De Zoysa

Czech J. Anim. Sci., 2023, 68(4):178-187 | DOI: 10.17221/132/2022-CJAS


The present study was designed to evaluate the effect of pectin supplemented feeds on the growth, gut morphology and immunology of Labeo rohita. Experimental feeds containing various concentrations of pectin including control (0%), T1 (0.5%), T2 (1.0%), T3 (2.0%), and T4 (4.0%) were administered to L. rohita twice per day at a rate of 3% body weight. There was a significant (P < 0.05) decrease in average final weight, weight gain%, net weight gain and specific growth rate observed in treatment groups in order of control > T1 > T2 > T3 > T4 while feed conversion ratio was significantly (< 0.05) higher in T4 compared to the other groups T3 > T2 > T1 > control. Immunological parameters of peroxidase, protease and antiprotease (trypsin) showed significant (< 0.05) results between control and treated groups. Peroxidase activity was significantly (< 0.05) higher in T4 group fish compared to T3 > T2 > T1 > control group. Total protein, protease and antiprotease activity were significantly (< 0.05) higher in the control compared to treatment groups T1 > T2 > T3 > T4. An increased level of the non-starch polysaccharide pectin in feed caused acute effects on gut morphometry. Villus length, villus width, and an increase in mucosal folds were abnormal but no significant (P > 0.05) abnormality was observed in the control group. In the submucosal part of the intestine, hyperplasia was shown in loose connective tissues and in adipose tissue cells in the treated groups. In conclusion, dietary pectin supplementation from 0.5% to 4% had negative effects on the growth, immunological parameters and gut histomorphology of L. rohita.

Improving the extraction efficiency and functional properties of wheat germ protein by ultrasound-assistedOriginal Paper

Chunyan Xie, Juan Du, Chunmiao Xing, Xu Zhang, Lan Wang, Hongzhang Chen, Tong Lin

Czech J. Food Sci., 2023, 41(2):118-126 | DOI: 10.17221/54/2022-CJFS

his study optimised the conditions for ultrasound-assisted extraction (UAE) of defatted wheat germ protein (WGP) and evaluated its effect on the functional properties. Single-factor and orthogonal experiment designs were combined to optimise the UAE extraction condition. The extraction of WGP reached the highest level, at 88.66%, with a solid : liquid ratio of 1 : 25 g·mL–1, pH value of 9.0, ultrasonic time of 10 min, and ultrasonic power at 400 W. Under these conditions, albumin, globulin, prolamin, and glutenin accounted for 32.26, 28.52, 5.42, and 22.40% of total protein, respectively. In addition, this study compared the functional properties of WGP extracted by UAE with the results based on a commercially available soy protein (SP) isolate (SPI). The UAE of WGP had better oil absorption, foaming, and emulsifying properties. Therefore, UAE is a promising technique for food protein extraction because it can change the protein efficiencies and properties of the extract.

Molecular diagnosis of red rot of sugarcane in Pakistan and in vitro control of isolated pathogen using rhizobacteriaOriginal Paper

Zimen Ahmad, Muhammad Asad Farooq, Amama Fatima, Syeda Gul Nisa, Sania Tabassum, Bushra Ismat, Sundus Waseem, Umer Iqbal, Asma Akbar, Urooj Haroon, Hassan Javed Chaudhary, Tauseef Tabassum, Ahmed Z Dewidar, Abdullah Alaklabi, Muhammad Farooq Hussain Munis

Plant Protect. Sci., 2023, 59(2):124-133 | DOI: 10.17221/45/2022-PPS

Sugarcane (Saccharum officinarum L.) is a key cash crop, and its production is influenced by a variety of phytopathogens in different parts of the world. During consecutive field surveys, sugarcane stalks with red rot symptoms were observed in three provinces of Pakistan (Punjab, Sindh, and Khyber Pakhtunkhwa). Cane samples with visible symptoms were plated on Potato Dextrose Agar (PDA) media. Morphological and microscopic observations identified this pathogen as Colletotrichum falcatum. Comparative molecular study of amplified sequences of β-tubulin (Bt) and Actin (ACT) genes showed 100% similarity and ITS sequence showed 99% similarity with C. falcatum. For in vitro control of red rot, eight bacterial strains from the rhizosphere of sugarcane were isolated. These strains showed variable growth inhibition of C. falcatum in dual culture method. Among all tested strains, Bacillus amyloliquefaciens and B. altitudinis exhibited best antifungal activities. Based on these results, tested bacterial strains (B. amyloliquefaciens and B. altitudinis) can be recommended as effective biocontrol agents to manage red rot disease of sugarcane.

Influence of primary tillage on the displacement of soil particlesOriginal Paper

Pavel Bro¾, Josef Hùla

Res. Agr. Eng., 2023, 69(2):101-106 | DOI: 10.17221/76/2022-RAE


The loss of soil particles due to erosion is one of the main problems of current agriculture. However, soil tillage may also contribute to the undesirable transport of soil particles. It is to note that the effects of particular working elements used on implements for soil tillage have not been described in a sufficient way. To determine the translocation of soil particles, measurements were done in the Central Bohemian region. Two basic machines for soil tillage were used for measurements: a disc harrow and a tine cultivator. Measurements were performed on sandy-loamy Cambisol after the harvest of a spring cereal crop. White limestone grit was used for the indication of soil particle translocation. Great translocation of soil particles was observed after soil tillage with a tine cultivator ‒ the most distant particles were found out at a distance of more than 1.50 m from the original location. After soil tillage with disc harrows, the most distant particles were found out at a distance of 0.90 m. The dependence of tracer weight on a distance from the original location could be described for disc harrows and tine cultivator by an exponential function.

Incidence of aflatoxin M1 in cows’ milk in Pakistan, effects on milk quality and evaluation of therapeutic management in dairy animalsOriginal Paper

I Ullah, A Nasir, M Kashif, A Sikandar, M Sajid, M Adil, A Rehman, MU Iqbal, H Ullah

Vet Med - Czech, 2023, 68(6):238-245 | DOI: 10.17221/18/2023-VETMED

The present study was aimed at measuring the concentration of aflatoxin M1 (AFM1) in the milk of Holstein Friesian cows, its effect on the milk quality and seasonal trends, as well as to investigate the efficacy of a commercial clay-based toxin binder. For this purpose, milk samples from dairy cows (n = 72) were collected and assayed for AFM1 before employing a clay-based toxin binder. The milk samples (n = 72) were collected from selected animals, revealing that 69.4% of the milk samples had AFM1 levels above the United States permissible limit (0.5 µg/kg). The incidence of AFM1 in milk during the winter and summer was 82.5% and 53.1%, respectively. Owing to the presence of AFM1, the level of milk fat, solids-not-fat, and protein were found to be low. Subsequently, the affected animals were divided into two groups, i.e., AFM1 positive control (n = 10) and the experimental group (n = 40). The experimental group of animals were fed the clay-based toxin binder at 25 g/animal/day. A progressive decrease of 19.8% in the AFM1 levels was observed on day 4 and on day 7 (53.6%) in the treatment group. Furthermore, the fat, solids-non-fat and protein increased significantly in the milk. In conclusion, a high level of AFM1 contamination occurs in the milk in Pakistan, affecting the quality of the milk production. Clay-based toxin binders may be used to ensure the milk quality and to protect the animal and consumer health.

Effect of onion waste powder on the rheological characteristics, sensory attributes, and antioxidant properties of mayonnaiseOriginal Paper

Tsvetko Prokopov, Kremena Nikovska, Milena Nikolova, Pavel Merdzhanov, Mina Dzhivoderova-Zarcheva

Czech J. Food Sci., 2023, 41(3):182-188 | DOI: 10.17221/19/2023-CJFS


The recovery and utilization of onion processing waste would contribute to the solution of environmental problems. This research presents the way of successful supplementation of onion processing waste in mayonnaise. Different levels (0, 1, 2, and 3%) of onion waste powder (OWP) were used as an additive to mayonnaise, based on sunflower oil. The final mayonnaise formulations were evaluated for sensory properties, rheological behaviour, oxidative stability, total phenolic content, total flavonoids, and antioxidant activity. The results indicated that OWP could be used as a potential health-promoting functional ingredient in amounts of 2% to produce mayonnaise enriched with total phenolic compounds and flavonoids, having high antioxidant activity, acceptable quality, and overall consumer preference.

Reliability of using Wood’s lamp by shelter personnel to diagnose Microsporum canis in catsOriginal Paper

K Mrazkova, J Konvalinova, I Bedanova

Vet Med - Czech, 2023, 68(7):281-286 | DOI: 10.17221/32/2023-VETMED


Optimising diagnostic methods in shelters so that they are as economical as possible for the shelter is especially important because shelters often have a significant lack of funds and so usually do not carry out preventive screening of cats. Dermatophyte fungi spread quickly and can infect shelter staff. The aim of our work was to identify the occurrence of Microsporum canis in shelter cats. It aimed to determine the prevalence of M. canis in cats at the selected shelter and compare the efficiency of detection using a Wood’s lamp and culturing on Sabouraud’s agar. All cats present in the shelter at the time of the study (n = 70) were examined with Wood’s lamp and hair sampling followed by subsequent culturing on Sabouraud’s agar. Identification of fungi was based on microscopic proof of macroconidia and microconidia. The prevalence of M. canis by diagnosis on Sabouraud’s agar was 64.29% of cats, with the help of Wood’s lamp 48.57% of cats showed positive fluorescence. The sensitivity of the Wood lamp examination was 71% and the specificity was 92%. Our study suggests that Wood’s lamp could be used by trained shelter personnel for the first examination of cats at reception and could significantly reduce the risk of spreading M. canis in shelters.

Seasonal dynamics and abundance of brown marmorated stink bug Halyomorpha halys (Stål) on four trap cropsOriginal Paper

Luka Batistiè, Tanja Bohinc, Stanislav Trdan

Plant Protect. Sci., 2023, 59(3):264-277 | DOI: 10.17221/2/2023-PPS

The main purpose of the study was to examine the potential of crops, such as soybeans (Glycine max), sunflowers (Helianthus annuus), alfalfa (Medicago sativa), and sorghum (Sorghum bicolor), as trap crops for Halyomorpha halys. We monitored the abundance and seasonal dynamics of H. halys (egg clusters, larvae, and adults) on a selected number of various crops at 10-day intervals. The experiment was performed in two different regions of Slovenia (western and central) and in two different years (2021, 2022). The results varied depending on the location and the year. In the first experiment, data from the entire year showed that sorghum was the most attractive for H. halys adults (1.56 ± 0.12), followed by sunflowers (0.61 ± 0.05), soybeans (0.37 ± 0.03) and alfalfa (0.41 ± 0.21). In the second experiment, the abundance numbers of the pest were significantly lower compared to those of the first experiment [sorghum and soybeans (0.003 3 ± 0.002), sunflowers (0.000 3 ± 0.000 3)]. In both cases, the abundance numbers increased when all the crops entered the fruit development stage (BBCH 70+). Overall, the lower density of the H. halys population and the experimental design could also be some of the factors for such a low incidence of stink bugs in the central region of the country. More research will be needed to further develop effective control methods for this invasive pest.

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