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A total of 29 520 animals, from 164 batches of pigs belonging to an identical number of herds, were involved in the study. The considered population of pigs were limited to the region of north-eastern Poland involving six voivodeships. From each herd, samples of blood were collected to evaluate the antibody titres to Mycoplasma hyopneumoniae, porcine reproductive and respiratory syndrome virus, Aujeszky's disease virus and swine influenza virus. At an abattoir, the lung lesions of each batch were scored and the enzootic pneumonia-like lesion average value was calculated. Lesions, indicative of enzootic pneumonia, were found in 57.8% of the lungs. For all lungs, the enzootic pneumonia-like lesion average value was 1.74, ranging from 0.42 to 3.56 among the 164 batches. In the examined pig population, 57.8% were considered suffering from swine respiratory disease, the majority of the affected pigs came from the Podlaskie (21.7%) and Greater Poland (17.25%) voivodeships. In the most affected voivodeships, 88.37% and 85.16% of the farms were considered as disease-susceptible for Greater Poland and Podlaskie, respectively. The findings indicate that, in pigs in north-eastern Poland, the major pathogens causing pneumonia-like lesions are Mycoplasma hyopneumoniae (68.9%) and porcine reproductive and respiratory syndrome virus (44%).

Clear-cutting is the most common silvicultural system. Sometimes, if the new crop is not established successfully, clearcut is left unreforested. This study focused on a site where early successional species such as silver birch (Bi) and rowan (Ro) were accompanied with Norway spruce (Sp) in 13-year-old stand from natural regeneration at 550 m of altitude at an acidic site with eastern aspect and 25% slope. We found five types of stand composition: treeless gaps, Ro-Bi, Ro-Bi-Sp, Bi-Sp and monospecific Sp. Besides these juvenile ones, adjacent 100-year-old spruce (Sp old) stand representing pre-harvesting conditions was studied. In addition to the performance of trees, organic layer (Hum), topsoil (Ah) and upper subsoil (B) horizons were sampled to study an expected shift of chemical properties after clear-cutting and secondary succession at the site of interest. Birch dominated the natural regeneration; rowan and spruce were present mostly in understorey. Old spruce was more acidic and nutrient-poorer compared to the juvenile treatments. The treeless treatment showed also slightly higher pH and comparable nutrients compared to the young mixtures. Young spruce was higher in nitrogen compared to Ro-Bi-Sp mixture.

Extensive microbiological research was carried out in Tbilisi (capital of Georgia) and its surroundings to determine the causes of massive disease and dieback of urban coniferous plantations. The biological material was picked up in June-July 2020 from trees with various degrees of the disease in 42 different localities. 247 conifers (15 species) were examined microbiologically. 1 169 samples of microscopic fungi were isolated. Based on cultural-morphological and molecular-genetic (PCR) studies, 34 strains were identified to the species level, 17 to the genus level, and 1 strain to the family level. Ascomycota were represented by 15 families and 33 species, Basidiomycota by 1 family and 2 species and Zygomycota by 2 families and 2 species. Among the isolated strains, 9 species were clearly dominant and found in all studied coniferous species: Alternaria alternata (Fr.) Keissl, Sphaeropsis sapinea (Fr.) Dyko & B. Button, Epicoccum nigrum Link., Sordaria lappae Potebnia, Curvularia spp., Dothiorella spp, Nothophoma quercina (Sydow & P. Sydow) Q. Chen & L. Cai, Phoma odoratissimi Q. Chen, Didymella aliena (Fries) Q. Chen & L. Cai. It may be supposed that massive activation of pathogenic fungi is the result of weakening of plant immunity on the background of increasing abiotic stresses in Tbilisi over the years; which led to an imbalance between latent pathogens and host plants and eventually to the depressing consequences of trees dieback.

While the benefits of using futures to manage price risk are widely recognised, only certain groups of farmers have suitable futures at their disposal. This paper discusses an innovative instrument, developed in the Belgian-Dutch pear market, that provides an alternative to futures markets by creating a market for price swaps. Thus, the instrument provides some benefits of market-traded derivatives (like futures) while remaining a relatively simple instrument, which requires fewer market transactions. The paper describes key properties of the swap contracts and the platform used to trade them. In addition, it compares the conditions required for establishing price swap markets and futures markets. Thus, our study informs the design of similar risk management instruments for commodities and contexts where futures are absent.

Spring barley varieties Tolar and Marthe were grown under the standard conditions and stress conditions with a combined effect of high temperature and drought in three experiments conducted in the greenhouse of phytotron type. The results showed that growing under the stress conditions led to reduced yield, grain quality and malt quality. This effect was observed both in the individual years and on average for the entire study period. Average yield of grain declined by ca 53% and retention above 2.5 mm screen decreased by ca 28% (P = 0.001). Further, average content of proteins rose by 3.7% while starch content decreased by 5.1% (P = 0.01). In malt samples, average extract and friability were reduced by 5.8% and 15%, respectively, and average protein content increased by 3.8% (P = 0.01). The growing conditions were a dominant factor in the conducted experiments.

Twelve adult Slovak warmblood sport horses were used to study the effect of dried grape pomace (DGP) on health through blood serum biochemical indicators, and on apparent total tract digestibility of dry matter (DM), organic matter (OM), crude protein (CP), acid detergent fibre (ADF) and neutral detergent fibre (NDF). The digestibility analysis was carried out by two in vivo methods, total faeces collection (TFC) and using lignin as a marker (ADL). Animals were divided into 3 groups: control group (C, without supplementation), experimental group 1 (E1, feed rations + 200 g of DGP) and experimental group 2 (E2, feed rations + 400 g of DGP). In animals, no health problems were detected during the trial. Of the blood serum indicators, only the concentrations of potassium (increase in E2 group compared to C group) and alanine aminotransferase (decrease in E2 group in comparison with E1 and C group) were affected (P < 0.05). The ADL method resulted in underestimated digestibility coefficients due to low recovery rates of lignin (less than 90%) in C group and E1 group. According to TFC, in E1 group higher digestibility coefficients were detected for DM, OM and CP (P > 0.05) compared to C group. However, in E2 group lower digestibility of all the studied nutrients was found (P > 0.05) in comparison with C group and E1 group. These results suggest that DGP could be used in horse diets up to 200 g without negative effect on their health and for a possible digestibility improvement of some nutrients.

To investigate the effect of microbial inoculum on soil heavy metal immobilisation, pot experiments were conducted with paddy soils contaminated by cadmium (Cd), lead (Pb), arsenic (As), and mercury (Hg), respectively. The results showed that the inoculation of Rhodopseudomonas palustris was more effective in the immobilisation of Pb and Cd in soils than the composite of R. palustris and Bacillus subtilis. Interestingly, a lower dosage of inoculum immobilised significantly more heavy metals than the higher dosage, potentially due to the competition of bacteria with limited nutrients. The heavy metal contents in rice grains also supported this finding, as less Pb and Cd were accumulated under the lower dosage. However, there were limited effects of microbial inoculations on the immobilisation of Hg and As. In general, our study indicated the effectiveness of R. palustris in immobilising Pb and Cd in soils and highlighted the importance of determining the optimal dosage of inoculum in bioremediation.

This study was conducted to evaluate the effects of a toy on the behaviour and body weight of weaned pigs after mixing. Two groups (192 pigs in total, control: without toy; treatment: with toy placed on the floor) of weaned pigs housed in pens (1.8 m × 1.4 m, 4 pigs/pen) were This study was conducted to evaluate the effects of a toy on the behaviour and body weight of weaned pigs after mixing. Two were observed with the aid of video technology for nine consecutive hours in the early (days 1 and 2) and late (days 38 and 39) period after mixing. Bodyweight of pigs at the end of the study (Mann-Whitney U-test, Z = -2.46, P = 0.01) and the average daily gain (Z = -2.75, P < 0.01) were 10% higher in the treatment group than in the control group. The presence of the toy in the pen influenced the pig behaviours. In pens enriched with toys, there was an increase in feeding behaviour and a decrease in agonistic and belly nosing behaviours. Toy supply reduced negative social behaviours and thereby improved the bodyweight of weaned pigs. The results of this study suggest that toys used as environmental enrichment are useful for enhancing pig welfare and effective for farmers.

Finding an appropriate method with the highest rate of polycyclic aromatic hydrocarbon (PAH) removal from naturally polluted soils is an important research issue. A pot factorial experiment (using contaminated soil samples from the Isfahan Refinery, Iran) was conducted in a 90-day period to compare the following bioremediation strategies: (1) natural attenuation (NA): the inherent ability of soil for bioremediation; (2) bioaugmentation (BA): inoculating soil with PAH degrading microbes Marinobacter hydrocarbonoclasticus; (3) biostimulation (BS): using N, P and K nutrients for the stimulation of bioremediating soil bacteria to achieve the C:N:P ratio of 100:10:1, and(4) bioaugmentation + biostimulation (BS + BA). Treatments NA (22.8%) and BA + BS (63.9%) resulted in the least and the highest rate of PAH removal from the soil. The 2-4 ring compounds had a significantly (P ≤ 0.05) higher rate of degradation than the 5-6 ring compounds. The highest rates were resulted by fluorene (76.41%) and acenaphthylene (72.28%) using the BA + BS treatment. However, the lowest degradation rates were resulted by indeno (1,2,3-cd) pyrene (10.05%), benzo [b] fluoranthene (10.17%), benzo (g, h, i) perylene (12.53%), and benzo [k] fluoranthene (13.67%), using NA treatment. The BA + BS treatments are the most effective method for the bioremediation of PAH polluted soils.

The effect of crop sequences (CR - continuous winter rye; CropR - three-field crop rotation of winter rye-spring barley-bare fallow) and fertilisation systems (unfertilised control, mineral fertiliser (NPK), farmyard manure (FYM)) on crop yield, energy efficiency indicators and land demand were analysed in a long-term experiment under Pannonian climate conditions. Due to lower fuel consumption in the bare fallow, the total fuel consumption for CropR was 27% lower than in CR. It was for NPK and FYM fertilisation by 29% and 42% higher than in the control. Although the energy output was lower in CropR than CR, the energy use efficiency for grain production increased by 35% and for above-ground biomass production by 20%. Overall crop sequences, the NPK treatment had higher crop yields, energy outputs and net-energy output with a lower energy use efficiency than the unfertilised control. CropR increased the land demand just by 20% in comparison to CR, although one-third of the land was not used for crop production. The land demand could be decreased with fertilisation by 50% (NPK) or 48% (FYM). A bare fallow year in the crop rotation decreased the crop yield, energy input and increased the energy use efficiency and land demand.

A straw chopper is a mechanical device used to uniformly chop fodder into small pieces to mix it together with other grass and then feed it to livestock. The objective of this research was to design and develop an animal fodder chopping machine to be utilised by dairy farmers within their purchase range. The drawing of these machine parts was undertaken in AutoCAD software and the construction was performed in a local workshop. After development of this machine, performance tests were carried out on a farm. The chopping machine tests were carried out with commonly grown fodder (namely: straw, grass, and maize) in Bangladesh. The performance evaluation of the developed machine was carried out in terms of the chopping efficiency, machine productivity, and energy consumption. The economic analysis of the straw chopping machine was assessed by indicating the cost effectiveness to the poor farmers. Analysis of the data in regard to chopping efficiency and machine productivity varied from 93 to 96% and from 192 to 600 kg×h^-1, respectively. The energy consumption during the chopping process ranged between 0.0025 and 0.01 kWh for the different types of fodder. The break-even point of the fodder chopping machine was 3 793 kg of cut straw and the payback period was within one year depending on the use.

In human medicine, skin grafting is an innovative surgical technique widely used in reconstructive surgery to repair skin loss. This case evaluated the effectiveness of a treatment with dermal micro-grafting obtained through the Rigenera^® technology in a chronic open wound resulting from a suture dehiscence of a limb amputation in a cat. Significant differences were observed between the aesthetic aspects of the injury using traditional treatments (cleaning and curettage) and the regenerative technology. The results showed that the healing periods were significantly reduced after the Rigenera^® treatment and that, moreover, a perfect skin status and a complete reduction in the wound area (100%) were achieved in one month. Given these results, Rigenera^® has proven to be a simple yet highly effective method in the treatment of inactivated chronic wounds.

The aim of the paper is to identify the factors influencing consumers when beer consumption and when choosing restaurants for beer consumption in the Czech Republic (CR). A partial goal was to compare preferences and factors influencing beer consumers from the Vysočina Region and the South-Moravian Region. The survey included a questionnaire survey, which 408 respondents took part in. The results showed that the most influential factors in the selection of beer for consumers are taste, a high-quality brand, and Czech production. Surprisingly, price was not found to be an influential factor in beer selection but is rather neutral. Factors that influence consumers most when choosing a restaurant for beer consumption were also identified. Consumers are most influenced by the quality of the beer on offer and the environment in which it is consumed.

In the last decade, the pig sector in Slovakia has been changing continuously, especially in regard to the proportion of pigs coming from foreign breeding programs. These changes lead to changes in carcass characteristics and to a change in the distribution of carcasses within the classes of the Union scale for the classification of pig carcasses. Therefore, this study was undertaken to analyse the actual state of pig carcass classification in the Slovak Republic and to investigate the needs of updating the equations for the classification methods and possible steps to improve the quality of pork produced within the country. Results showed that an increased number of pig carcasses originated from Danish breeding program, and also from the Czech Republic and Poland. When compared to carcasses in 2009, these changes resulted in lower backfat thickness and higher muscle thickness of the longissimus dorsi muscle and thus higher lean meat content of carcasses. This results in a higher number of carcasses classified in the S and E classes. Slight differences between two instrumental classification methods were calculated. The study showed the need for updating the equations for classification methods, and also suggested possible further steps in order to improve the quality of pork.

The study aimed to estimate and characterise soil organic matter under different land uses (cropland, grassland, and forest) and soil depths. The soil organic matter composition of the soil was assessed by diffuse reflectance infrared spectroscopy (DRIFT). Humic and fulvic acids (HAs, FAs) were extracted from soils and their compositions were evaluated by DRIFT. Low molecular mass organic acids content was also measured. Our result revealed that the largest differences of the spectra in the composition of organic matter were observed in the upper parts of the soil profile. The forest soil spectra had more intense aliphatic bands, carboxylic, and CH bands than spectra of grassland and cropland soils. The difference of HAs spectra was at 3 010 to 2 800/cm where the most intensive aliphatic bands were in forest soil HAs, followed by grassland and cropland soil HAs. The grassland topsoil FAs spectrum differs most from the other land uses. It has lower peaks around 1 660-1 600/cm and 1 200/cm than cropland and forest. The concentration of low molecular mass organic acid (LMMOA) was the highest in the forest soil and the most abundant acid was citrate.

Fine root traits are plastic and responsive to increased nitrogen (N) deposition. However, with the restoring of the ecosystem after grain for green, little research has been reported about the response of root traits in a long-term restored ecosystem to increased N deposition. Therefore, a successive N addition experiment was conducted in a long-term restored grassland on the Loess Plateau to analyse the effects of different N addition levels (0, 2.5, 5, 10, 20 g N/m²/year) on root morphological traits, soil carbon (C) and N. Our results showed that root morphological traits (except for root diameter) firstly increased and then declined, with the maximum in the N level of 5 g/m²/year. N addition significantly increased soil organic carbon, total nitrogen, ammonium nitrogen (NH₄⁺-N) and nitrate-nitrogen (NO₃^--N) with the increasing N addition level, especially in the soil surface layer. Specific root length and specific root area had remarkable negative correlations with NO₃^--N, while root diameter and root length density had positive correlations with soil availability N and soil microbial biomass carbon. This study indicated that plants could have the threshold response to adapt to the N addition and prefer to slowly grow rather than quickly invest and return in order to adapt to the environmental stress.

Dog barking, which reaches around 100 dB, often becomes a noise issue in urban environments. One potential solution, a simple soundproof cage, has recently been marketed. To our knowledge, no study has been conducted to investigate what sort of stress burden is imposed on dogs kept in cages, and it may raise animal welfare concerns. The purpose of this study was, therefore, to reveal whether staying in a soundproof cage caused stress for the dogs or not. Ten healthy domestic dogs (5 males, 5 females) of small body size were evaluated. The heart rate variability (HRV), behaviour, and internal and external temperature during confinement in a soundproof cage for each dog were analysed. The HRV analyses revealed no significant differences for any variables between confinement and non-confinement (HR, P > 0.999 9; rMSSD, P = 0.359 4; SDNN, P = 0.359 4; LF, P = 0.652 3; HF, P = 0.128 9; LF/HF, P = 0.222 7). Overall, in our behavioural analysis, there were no significant differences between confinement and non-confinement (P = 0.105 5). In conclusion, the HRV and behavioural analyses did not indicate an increased stress burden on the dogs during confinement in the soundproof cage compared with non-confinement. The inner temperature of the cage was not elevated either. Nevertheless, the stress imposed on dogs caused by a soundproof cage should always be considered on an individual basis, and the time spent in such a cage should always be kept to a minimum.

The population structure of Phytophthora capsici among asexual and sexual progenies was analyzed using ISSR. Thirty asexual progenies of one parent and three sexual populations were assayed for genetic diversity using 5 ISSR primers and DNA from 120 offspring of P. capsici was amplified. In total, 71 reproducible ISSR fragments were obtained, of which 100% were polymorphic, revealing high polymorphism among the isolates. Among them, the percentages of polymorphism of sexual and asexual progeny isolates were 100.00 and 77.46%, respectively. Genetic similarity coefficients among all the isolates ranged from 0.54 to 0.73. The sexual offspring population showed much more variability than the asexual offspring population with 76.26% variability attributed to diversity within populations as compared with 23.74% among populations. This research reveals that the sexual progeny population of P. capsici contributes more genetic diversity than that of asexual progeny population.

Current research work was performed to evaluate the antioxidant, antidiabetic, thrombolytic, and cytotoxic potential of Bougainvillea glabra leaf extracts with different solvents. Extraction of leaves was carried out by maceration using solvents of various polarity such as n-hexane, chloroform, ethyl acetate, n-butanol, and methanol. The highest values of total phenolic and flavonoid contents were assessed in methanolic extract, such as 58.57% and 29.41%, respectively. Antioxidant potential was measured by DPPH free radical, showing 65.16% scavenging activity in methanolic extract. Similarly, the reducing power of methanolic extract was also measured. Hemolytic activity of B. glabra leaf extracts was evaluated, and maximum percentage lysis was found as 6.86% in chloroform extract. The thrombolytic activity of B. glabra leaf extracts was evaluated against human red blood cells, and the maximum percentage of clot lysis was 59.10% in chloroform extract. Maximum antidiabetic activity (16.20%) was observed in methanolic extract. Overall, the presented results reveal that bogan bail extract is capable of being employed in nutra-pharmaceutical industry.

Slow-release nitrogen fertiliser can potentially increase crop production and improve fertiliser nitrogen use efficiency. However, it is unclear that are suitable for different regions and crops in the northeast of China. Therefore, according to different soil and climate characteristics, we investigated the synchronised relationships between nitrogen slow release fertiliser and nitrogen maize requirements. Experiments were conducted at Shenyang Agricultural University, Liaoning province, Northeast China, from 2016 to 2017. Stabilised fertiliser treatments increased grain yield, nitrogen use efficiency and nitrogen accumulation at each maize growth stage. Grain yield increased by 2.32% and 11.33% (2016), and 1.55% and 7.87% (2017), respectively, when compared with the urea CK1 (233 kg N/ha) and CK2 (210 kg N/ha) treatments. Additionally, during the growth period of the stabilised fertiliser treatment, the stability of the synchronisation relationship between nitrogen absorption and absorption of spring maize was significantly higher than other treatments, and the effect was the best. Therefore, we conclude that the stabilised fertiliser is the most suitable option for promotion and application in spring maize in Northeast China.

This study compared different methods of determining starch digestibility (in vivo vs in vitro) in wheat grains and evaluated the influence of extrusion on digestibility. In vivo starch digestibility was determined in broiler chickens by calculating the residual starch content in their ilea and the digestibility using a chromium oxide indicator. In vitro digestibility was examined using pepsin and pancreatin. During in vivo testing, the highest digestibility coefficient (DC) was achieved by the Bonanza variety in its extruded form (91.19 ± 0.40%). In contrast, the lowest DC was achieved by the Tobak variety in its non-extruded form (81.45 ± 1.92%). Generally, a higher DC was observed in vivo for extruded forms of wheat. During in vitro testing, the highest DC was achieved by the Stefii variety in its non-extruded form (96.10 ± 0.55%), whereas the lowest DC was observed in the Yetti variety in its extruded form (49.72 ± 0.41%). Overall, the in vitro experiments did not exhibit significant differences between extruded and non-extruded forms of wheat. Linear regression analysis showed a strong relationship (r² = 0.860; 85.98%) between in vivo- and in vitro-derived DC values in all wheat varieties, both in extruded and non-extruded forms. The study showed that in vivo testing is a suitable method for the determination and control of starch levels in extruded materials. However, despite the accuracy of this technique, it is also very demanding in terms of time, space, equipment, and methodological knowledge. Therefore, based on the strong correlation between the in vivo and in vitro assays, we recommend in vitro digestibility testing as a preferable alternative.

Intercropping system plays a crucial role in improving crop yield, nitrogen utilisation efficiency (NUE) and economic benefit. The difference in crop yield and interspecific relationship under different bandwidth and row ratio allocation patterns are still unclear. A field experiment was carried out to explore change regularities between crop yield and interspecific relationships under maize soybean intercropping with different bandwidths and row ratios. The results showed that the yield of intercropped crops was lower than that of the sole crop. The nitrogen accumulation (NA), NUE and nitrogen competition ratio was the highest under the intercropping mode with a bandwidth of 2.0 m, which indicated that this mode was more conducive to the N uptake and utilisation in crops. In all intercropping systems, nitrogen equivalent ratio (NER) and land equivalent ratio (LER) were all greater than one, indicating that intercropping systems were conducive to improving land utilisation efficiency and NUE. Under the same bandwidth pattern, expanding the maize soybean row ratio from 2:4 to 3:4 was beneficial to the improvement of LER, NER, NUE, crop group yield. In conclusion, it was preferable in the NA, NUE, crop group yield under the system of bandwidth 2.0 m and row ratio 2:2, which could be a reference for maize soybean intercropping system.

The aim of this study is to evaluate the outcome of the treatment on twenty wild birds presenting with fractures that were stabilised with modified Meynard external fixators. The study material consisted of a total of twenty birds of eight different species, six white storks (Ciconia ciconia), one eastern imperial eagle (Aquila heliaca), one European honey buzzard (Pernis apivorus), one long-legged buzzard (Buteo rufinus), three common buzzard (Buteo buteo), one northern goshawk (Accipiter gentilis), one Armenian gull (Larus armenicus), and six yellow-legged gulls (Larus michahellis), which were diagnosed with fractures in their extremity bones following clinical and radiological examinations. The lesions of the twenty cases evaluated in the study were diagnosed as humerus fractures in nine cases, ulna fractures in three cases, tibiotarsus fractures in seven cases and a femur fracture in one case. The external fixators were removed between weeks five and nine postoperatively. While healing was seen in twelve of the twenty evaluated cases, the complete functional recovery occurred in six of these and they were released into nature. It was concluded that modified Meynard external fixator, which was made applicable for fractures in birds, could be an alternative method for fracture treatments in wild birds.

Farms generally produce products with low differentiation. To reach an increase in the profit and sales, product differentiation is one of the potential strategies that could be successfully applied. This paper identifies "Intermediate Consumption/Total Output" as a simple and user-friendly indicator for farm product differentiation performance and comparison assessment in the Czech Republic, based on a two-step cluster analysis performed on 1 225 farms with different operating conditions and reproduction process characteristics in the Czech Republic. The data are sourced from Farm Accountancy Data Network (FADN CZ). Four farm clusters based on product differentiation levels are identified and described from the point of view of the production process and conditions. The resulting cluster profiles, in general, indicate the production conditions and process affecting the resulting product differentiation. Nevertheless, farms deliver extraordinary product differentiation values in fields with less favourable conditions and production processes. Those have the potential to be an inspiration for farms with lower product differentiation values. The result of this paper provides hope, less favourable conditions are not a limit for formidable performance. This paper result can be practically applied by anyone aiming to easily identify, evaluate, and compare farm product differentiation levels.

Abstract: The aim of this study was to analyse the production of gluten-free, low protein cookies, which can easily be consumed by celiac and phenylketonuria (PKU) patients. The formula was adopted to prepare the control cookies (without dried fruits) from maise starch, stabilisers, salt, sodium bicarbonate, protein-reduced milk powder, and sunflower oil. Different cookies were made by adding raisins (R), dried black currants (Cu), dried dates (D), dried apples and cinnamon (A) separately. In cookies, two different stabilisers were calculated according to starch weight [pectin (P): 0.15%, glycerol monostearate (GMS, G): 0.4%]. The effects of stabilisers and dried fruits on the physicochemical and sensory properties of cookies were investigated. The protein content was significantly higher (P < 0.05) in the cookies including R with pectin (PR), Cu with pectin (PCu), and A with pectin (PA). The amount of phenylalanine (Phe) was significantly higher (P < 0.05) in PR. The values of dietary fibre were the highest in the A cookies, while the lowest values appeared in the control (C). In the sensory evaluation (appearance, tasting properties, and affordability), it was found that PD (pectin with dries dates) was chosen as the best cookie (P < 0.05).

A new food and feed self-sufficiency model for the Czech Republic (RESTEP) was applied for the evaluation of possible adverse climate impacts uniformly reducing crop production by 5, 10, 20, and 30%. The situation was simulated for the whole country and four different agriculturally important regions. Biomass production modeling confirmed that for the whole country, the food self-sufficiency is secured up to 20% of yield decline for most crops, but even 5% yield decline of silage maize would lead to its shortage in animal feeding. On the other hand, regional results vary significantly. Regions Jižní Morava and Střední Čechy shown oversupply of feedstuff allowing them to cover the demands of cattle and pigs up to 20% or 30% decline of yield, respectively. The opposite model represents the Vysočina (VY) region which is not able to cover the demands from own sources even at the baseline scenario. The acreage extension of maize is not possible due to erosion risk restrictions at 25% of arable land at VY. The possible solution consists of extension acreage of alfalfa and clover or finding other plants sufficient for feeding as well for biogas facilities in regions rich in biomass energy consumers.

Cadmium (Cd) is a toxic heavy metal, discharged into environments naturally or anthropogenically, where industrial and agricultural revolutions have led to serious Cd pollution into the environment. Poultry feed may be contaminated by Cd due to its presence in concentrates and supplements, and also in the machinery and equipment used in feed production and processing. After absorption in the digestive system, Cd largely accumulates in kidneys, liver, bones and other organs and causes irreversible damage to these organs. Higher doses of Cd in the poultry diet reduced feed intake, body weight, bone mineral density, egg production and eggshell quality. Selenium (Se) is an essential micronutrient and important component of a variety of antioxidant enzymes, with the ability to antagonize metal toxicity including Cd. Hence, in the current review, we try to summarize recent information about the toxicological effects of Cd and provide updated information about the role of Se as an effective strategy in reducing Cd toxicity in poultry.

The honey bee is one of the insects that is significantly endangered by the application of pesticides in the cultivation of crops. Not only is acute toxicity dangerous, but the importance of chronic poisoning by low doses of pesticides in hives is growing. The behavior of bees can be affected not only by insecticide residues but also by herbicide and fungicide residues. In 2016-2018, samples of bee bread were analysed for pesticide content at 25 different localities from intensive agricultural production areas of the Czech Republic. Substances were extracted by QuEChERS and determined by liquid chromatography, together with mass spectrometric detection. We detected up to 18 pesticides in one sample. In total, during 2016-2018, we identified 53 active substances. Fifteen substances (31%) were herbicidal, 23 substances (47%) of fungicidal nature and 6 substances (12%) of insecticidal nature. The coefficient of variation showed large differences in the frequency of revealed pesticides between years. For substances sprayed outside period attractive for pollinators (mainly herbicides and some fungicides), the usual methodology cannot reliably determine the degree of contamination, and thus the actual contamination with these substances may be even higher than demonstrated in this study.

A congenital defect that blocks the cerebrospinal fluid outflow is the most common cause of hydrocephalus in young cats. It usually manifests itself within a few weeks of birth. Diagnostic imaging is essential. Therefore, it was decided to report on, for the first time, a clinical case of congenital hydrocephalus in a Maine Coon foetus detected using ultrasound during pregnancy. A difference between the size of the skull bones in the Maine Coon foetuses was recognised during a routine pregnancy ultrasound. Analysis of the sonogram of the bones and brain structures of the diagnosed foetus revealed that the features were highly suggestive of a hydrocephalus. Five clinically healthy kittens and one with hydrocephalus were delivered without complications. The necropsy of the malformed foetus was consistent with the ultrasonographic findings. Bacteriological and virological tests were performed and all were negative which suggest a congenital background of the malformation. This is the first reported case of an intrauterine ultrasound diagnosis of congenital hydrocephalus in a Maine Coon of multiple gestation. The ultrasound control along with the foetometry seem to be useful tools to recognise certain pathologies of pregnancy in cats, including congenital malformations of various origins, which is of significant diagnostic and prognostic importance.

Currently, it is difficult to identify correct data on wood sources and their consumption in the Czech Republic. Official statistics of wood production are relatively limited to obtaining data that accurately captures the objective wood production, which would enable the observation of the direction or consumption of particular tree species or products. This uncertainty is then transferred to the wood flow for industrial and energy processing, and it is not possible to compose a detailed picture of how much wood and which sources enter into the wood processing and how large the total material flow is. The aim of this paper is to present an option for how to determine this lack of valid information that enables clear outcomes necessary for decision making by means of the recalculation of wood sources consumed in the Czech Republic, using the data of processing capacities and foreign trade. The reverse model better expressed a conversion value of roundwood into the products and allowed optimal approximation in the wood sources assessment.