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Radiographic findings in sheep with abomasal phytobezoariasisOriginal PaperS.M. Hashemiasl, S. Azizi, D. TorkamaniVet Med - Czech, 2016, 61(8):436-442 | DOI: 10.17221/9/2016-VETMED The purpose of this study was to characterise the radiographic appearance of abomasal phytobezoars in sheep as well as to evaluate the utility of abdominal radiography to identify them. Twenty-seven fat-tailed Herrick sheep with a clinical suspicion of abomasal impaction were examined radiographically. Abdominal survey radiographs in right lateral recumbency were taken. Abomasal phytobezoars (AP) were seen in abdominal survey radiographs in 25/27 sheep (92%). Their radiographic survey appearance was round-to-oval masses with radiopaque margins and radiopacity similar to the ingesta centrally. An additional gastrographic barium study was performed in six of the sheep, followed by exploratory laparotomy where phytobezoars were removed through abomasotomy. The optimal time to visualise the APs was 48 h post-contrast. A significant correlation was noted between phytobezoars size in radiology and surgery (r = 0.651, P < 0.001). Use of the barium study can improve the phytobezoar-ingesta contrast and visibility of the phytobezoars. Plain radiography with sheep positioned in right lateral recumbency is a useful supplementary technique which can be used to evaluate abomasal phytobezoariasis. This study shows that radiography is a suitable diagnostic method for detecting the presence of, but not the number of, abomasal phytobezoars in sheep. |
Values and professional and ethical norms in rural tourism in PolandOriginal PaperJan SIKORA, Wlodzimierz KACZOCHA, Agnieszka WARTECKA-WAZYNSKAAgric. Econ. - Czech, 2015, 61(8):377-392 | DOI: 10.17221/14/2014-AGRICECON With development of the market economy, the views concerning the importance of ethical norms in business activity are developing. Ethical orientation in economics, has been popularized for several decades by e.g. French centre "Economie et Humanisme"1, established by a renowned Dominican, L.J. Lebret. Tourism, including rural tourism, represents an economic activity which has its specific ethical dimension. The goals of this paper are to characterize the basic values that are voluntarily accepted and used in tourism in rural areas in Poland and to identify the ethical norms that guarantee the realization of these values. The authors adopted the basic values-goals that should be recommended in rural tourism. They also formulated specific professional and ethical norms that support realization of the values, which include: humanity and standards for realizing the principle of humanity; the cultural and material conditions of living in rural areas; economic activity, including tourist activity that improves rural living conditions; innovative attitudes towards modernization and improving the quality of rural tourist services; and values that support the development of sustainable tourism in rural areas. The theoretical investigations concerning axiological problems were supplemented with the results obtained from a national-level empirical survey that was conducted in rural areas in Poland. |
Determination of fungal pathogens associated with Cuminum cyminum in TurkeyOriginal PaperGöksel ÖZER, Harun BAYRAKTARPlant Protect. Sci., 2015, 51(2):74-79 | DOI: 10.17221/51/2014-PPS The occurrence of fungal pathogens, associated with cumin production of Turkey, was determined during 2011 and 2012. A total of 379 isolates were classified as Fusarium oxysporum f.sp. cumini, F. solani, F. acuminatum, F. equiseti, F. sambucinum, F. avenaceum, Macrophomina phaseolina, Alternaria burnsii, A. alternata, A. infectoria, Embellisia sp., and Rhizoctonia solani. Pathogenicity tests showed that F. oxysporum f.sp. cumini, F. solani, F. equiseti, M. phaseolina, A. burnsii, and A. alternata were highly pathogenic. A. burnsii and F. oxysporum f.sp. cumini were the major risk factors for cumin cultivation in Turkey. To our knowledge, this is the first report on F. oxysporum f.sp. cumini, F. solani, F. equiseti, M. phaseolina, and A. alternata in Turkey. |
Heritability of powdery mildew and scab resistance within apple progeniesOriginal PaperJan Blaľek, Lubor Zelený, Jana KřelinováPlant Protect. Sci., 2016, 52(2):123-127 | DOI: 10.17221/130/2015-PPS The occurrence of both diseases was evaluated for four years in a selected orchard where 22 different apple progenies, totalling 699 seedlings were planted. During this period, no chemical protection against the diseases was applied in the orchard, and the incidence of the diseases was rated using a 9-point scale (from 9 = not infected to 1 = very highly infected). For the finalisation of the results, each seedling was characterised by the highest rate of infection from all four years of the evaluation. The lowest mean level of mildew infection equal to 7.47 was observed in the progeny of Resista × McIntosh Wijcik. Concerning the scab, the best was the progeny of Resista × Karmína having a rating equal to 8.27. The highest level of segregation of seedlings having joint resistance to both diseases was found out in the progenies obtained by crossings of HL665 × HL782 and Resista × HL447. |
Antioxidant activity, S-alk(en)yl-l-cysteine sulfoxide and polyphenol content in onion (Allium cepa L.) cultivars are associated with their genetic backgroundFood Chemistry and SafetyKatarína Mitrová, Vojtěch Hrbek, Pavel Svoboda, Jana Hająlová, Jaroslava OvesnáCzech J. Food Sci., 2016, 34(2):127-132 | DOI: 10.17221/268/2015-CJFS Six onion cultivars Bingo, Dormo, Elenka, Elbrus, Spirit, and Sturon grown in the Czech Republic for commercial purposes were analysed to investigate the content of health-promoting compounds. The results showed that at harvest time, cysteine sulfoxide content varied from 32.38 to 44.16 g/kg of dry weight, polyphenol content was between 2.66 and 3.37 g/kg of dry weight, and antioxidant activity ranged from 0.75 to 0.83 g/kg. Cv. Bingo had the highest level of the analysed compounds. The cultivars were concurrently analysed by DNA (microsatellite) markers. Dendrograms based on the chemical composition and DNA analysis were almost identical. This finding confirms the dependence of the secondary metabolite content on onion genotype. |
Winter wheat: results of long-term fertilizer experiment in Prague-Ruzyně over the last 60 yearsOriginal PaperL. Hlisnikovský, E. Kunzová, L. MenąíkPlant Soil Environ., 2016, 62(3):105-113 | DOI: 10.17221/746/2015-PSE The study evaluates how sixty years of application of organic manures and mineral fertilizers (ten fertilizer treatments altogether), planting of cultivars with different length of stem (long- and short-stem cultivars) and preceding crops (potatoes and alfalfa) affected grain and straw yields of winter wheat in the Prague-Ruzyně long-term fertilizer experiment (RFE). Fertilizer treatments did not affect grain yield during the first ten years of the RFE experiment (1959-1968), but influenced straw yield. The grain yield ranged from 5.08 (control) to 5.43 (farmyard manure) t/ha, straw yield varied from 6.02 t/ha (control) to 8.31 t/ha (poultry slurry (PS) + N4P2K2). In the last ten years of the RFE experiment (2004-2013) grain yield ranged from 7.01 t/ha (control) to 8.88 t/ha (stale + N4P2K2), while straw yield decreased and varied from 3.12 (control) to 6.21 t/ha (PS + N4P2K2). Comparing the potatoes and alfalfa as preceding crops, the grain yield was 0.5 t/ha higher after alfalfa, but straw yield was 1.3 t/ha higher after potatoes. Introduction of short-stem cultivars increased average grain yield about 2 t/ha and decreased average straw yield about 0.85 t/ha. |
Response of soybean (Glycine max (L.) Merr.) to bacterial soil inoculants and foliar fertilizationOriginal PaperW. Jarecki, J. Buczek, D. Bobrecka-JamroPlant Soil Environ., 2016, 62(9):422-427 | DOI: 10.17221/292/2016-PSE Soybean yields can be considerably improved by inoculation with selected Bradyrhizobium japonicum strains and foliar fertilization. An exact field experiment was carried out in 2012-2014 at the Experimental Station of Cultivar Assessment in Przecław, Poland. The test plant was soybean cv. Aldana. The experimental factors were: bacterial inoculant Nitragina (Bradyrhizobium japonicum); foliar fertilization with Mikrokomplex; combined applications Nitragina + Mikrokomplex and the control treatment. Significant effect of Nitragina on an increase in the number of plants prior to harvest, plant height and the number of pods per plant was indicated. Fertilization with Mikrokomplex caused an increase in the number of pods per plant and thousand seed weight. Nitragina + Mikrokomplex increased the number of plants prior to harvest, plant height, the number of pods per plant and thousand seed weight. Seed yield was significantly higher in all the treatments compared to the control (2.31 t/ha). Higher soil plant analysis development values were found after the application of Nitragina + Mikrokomplex, and in the stage of pod development, also after foliar fertilization with Mikrokomplex. Application of Nitragina and Nitragina + Mikrokomplex resulted in an increase in leaf area index and mean tip angle and total protein in seeds. Fe content in seeds was the lowest in the control (69.2 mg/kg) and significantly higher in the other treatments (Nitragina, Nitragina + Mikrokomplex), and Mg content significantly increased after the application of Mikrokomplex and Nitragina + Mikrokomplex. |
Maternal immunity induced by inactivated S. Typhimurium vaccine is less protective to S. Derby challenge than to S. Typhimurium challenge in suckling pigletsOriginal PaperJ. Gebauer, A. Osvaldova, H. Kudlackova, M. Maceckova, F. Sisak, H. Havlickova, P. Ondrackova, L. Leva, M. Faldyna, J. MatiasovicVet Med - Czech, 2016, 61(1):23-27 | DOI: 10.17221/8679-VETMED Salmonella enterica serovar Typhimurium and Salmonella enterica serovar Derby are the most common serovars of Salmonella enterica ssp. enterica found in pigs in Europe. We previously observed that suckling piglets of sows vaccinated with an S. Typhimurium-based inactivated vaccine are protected against homologous strain challenge. To develop this vaccine for commercial use, potential crossprotectivity of this vaccine to challenge with S. Derby was tested. Two sows were vaccinated with an S. Typhimurium-based inactivated vaccine while two other sows remained serologically negative. Four-day-old suckling piglets from both groups were orally challenged with S. Derby or S. Typhimurium. Maternally-derived immunity against S. Typhimurium protected piglets against S. Typhimurium challenge, when a significant (P < 0.05) decrease in S. Typhimurium count was found in ileocaecal and submandibular lymph node, tonsil, ileum and ileum content. On the other hand, after S. Derby challenge, significant (P < 0.05) decrease in S. Derby count was detected only in ileum content. Although both serovars belong to the same O:4 serogroup, other antigenic structures, for example the flagellin, are different. In a subsequent in-vitro experiment, we found that serum from vaccinated sows inhibited the motility of S. Typhimurium but not the motility of S. Derby. Our results indicate that protectivity of S. Typhimurium vaccine against S. Derby infection is limited. |
The practical use of computed tomography in evaluation of shell lesions in six loggerhead turtles (Caretta caretta)Original PaperF. Spadola, G. Barillaro, M. Morici, A. Nocera, Z. KnotekVet Med - Czech, 2016, 61(7):394-398 | DOI: 10.17221/68/2015-VETMED Six adult loggerhead turtles were found stranded and were rescued near Sicily within a period of 12 months. Macroscopically apparent lesions of the shell were present. After thorough physical examination, ketamine-dexmedetomidine-atipamezole induction and tracheal tube insertion all six patients underwent computed tomographic examination under inhalant anaesthesia with isoflurane. A vertebral lesion at the level of the 3rd thoracic-lumbar vertebra with vertebral lamina and the vertebral body being involved without compression of the spinal cord, a vertebral lesion at the level of the 7th thoracic-lumbar vertebra and a vertebral lesion at the level of the 8th thoracic-lumbar vertebra were recorded in the first female. Loss of the shell near the left carapace-plastron bridge, with massive haemorrhage and compression of organs were present in the second female. The remaining four turtles had only superficial lesions with no involvement of bones and organs of the coelom. Computed tomography was proved to be a valuable non-invasive method for clinical examination of stranded sea turtles. |
Influence of agricultural crops adjacent to forest on woody species browsing: Is it advantageous to have a tasty neighbour?Original PaperJ. Kamler, M. HomolkaJ. For. Sci., 2016, 62(1):41-46 | DOI: 10.17221/71/2015-JFS Preference of biotopes and selection of food by wild herbivores are more complicated in a cultural landscape used for agriculture than in natural conditions. Agricultural management significantly affects the feeding as well as cover conditions of the environment and changes in quality and accessibility of food sources occur throughout the year. We studied the consumption of agricultural crops and impact on tree shoots by herbivores in a mosaic landscape. Large herbivores utilized the food supply on the lands outside the forest environment during winter and early spring and in summer. In summer the cereal grains were preferred by herbivores and accounted for a significant share in their diet. The intensity of shoot browsing differed between the localities and between the studied woody species but was not lower due to the presence of attractive crops near the forest. The utilization of food outside the forest may help the animals to survive the winter but the potential of this food for reducing tree browsing in the forest is low. |
INDEX OF VOLUME 62IndexeditorsJ. For. Sci., 2016, 62(12):I-V | DOI: 10.17221/9380-JFS |
Eggshell structure, measurements, and quality-affecting factors in laying hens: a reviewReviewM. Ketta, E. TůmováCzech J. Anim. Sci., 2016, 61(7):299-309 | DOI: 10.17221/46/2015-CJAS Eggshell quality is one of the most significant factors affecting poultry industry; it economically influences egg production and hatchability. Eggshell consists of shell membranes and the true shell that includes mammillary layer, palisade layer, and cuticle. Measurements of eggshell quality include eggshell weight, shell percentage, breaking strength, thickness, and density. Mainly eggshell thickness and strength are affected by the time of egg components passage through the shell gland (uterus), eggshell ultra-structure (deposition of major units), and micro-structure (crystals size and orientation). Shell quality is affected by several internal and external factors. Major factors determining the quality or structure of eggshell are oviposition time, age, genotype, and housing system. Eggshell quality can be improved through optimization of genotype, housing system, and mineral nutrition. |
Climate change impacts on the Alpine ecosystem: an overview with focus on the soilReviewS. Chersich, K. Rejąek, V. Vranová, M. Bordoni, C. MeisinaJ. For. Sci., 2015, 61(11):496-514 | DOI: 10.17221/47/2015-JFS The Alpine ecosystem is very sensitive to climatic changes, which have an influence on glaciers, snow, vegetation and soils. The aim of this review is to illustrate the effects of global change on the Alpine soil ecosystem, which is an optimal marker to record them. The manuscript enhances our understanding of the global change effect on the Alpine environment: on morphology, on ice, on vegetation and points out how the cycles of soil nutrients equilibrium have been changed with a direct effect on soils that support plant species. The changes in cryosphere, glacier reduction and periglacial environment as glaciers retreat, decrease in the snow cover extent and earlier snowmelt, determine an effect on soils (on the structure, organic matter and humus forms, soil processes and soil types) from the top of the Alpine horizon to the bottom. The processes induced by climate change (such as erosion and tree line shifting) have a direct effect on water balance that can be observed on soil profile characters with an effect on upward migration, change in phenology, extensive losses of species. The equilibrium of the biogeochemical cycles has been changed and this has a direct effect on soils that support plant species. |
Mesh repair of a large ventral hernia with interposition of omentum in a calf: a case reportCase ReportG. Giusto, C. Bellino, M. Casalone, V. Caramello, F. Comino, M. GandiniVet Med - Czech, 2016, 61(10):590-593 | DOI: 10.17221/272/2015-VETMED A one-month-old, Piedmontese female calf was admitted to the Department of Veterinary Sciences, University of Turin, for repair of a large ventral hernia. A large ventral hernia, approximately 20 cm long and 15 cm large was noticed extending from 3 cm caudal to the umbilicus down to the pubis. At ultrasonography the hernia content was represented by small intestine and omentum and no adhesions to the hernial sac could be detected. The hernial sac was composed by skin only. Because of the large dimensions of the defect and the economic value of the animal, surgical correction was recommended to the owner. A prosthetic implant with a polypropylene mesh was elected due to the dimension of the abdominal defect. The implant was placed intra-abdominally with the interposition of the omentum between the mesh and the underlying viscera. Two and six months after surgery follow-ups were performed and a positive outcome was confirmed. This is the first report of ventral hernia repair in large animals that combines the use of a tension-free polypropylene mesh with the interposition of the omentum between the viscera and the mesh. This procedure is safe, cost-effective and not associated with major complications. |
Historical development of land ownership in the Czech Republic since the foundation of the Czechoslovakia until presentShort CommunicationLudek HOMOLAC, Karel TOMSIKAgric. Econ. - Czech, 2016, 62(11):528-536 | DOI: 10.17221/250/2015-AGRICECON The aim of the paper is to describe the land ownership relations in the area of the current Czech Republic since the foundation of Czechoslovakia. The description of the history of changes of land ownership in the Czech Republic is important for the perception of the current nature of the land use and land market. Land use and ownership was always related to the historical context and political regime. The time periods are divided by the important historical events: after 1918 (foundation of the Czechoslovak Republic), 2nd World War period, after 1948 (takeover of the power by the Communist Party of Czechoslovakia and the centrally planned economy period) and after 1989 (foundation of the market economy). During this era, three land reforms took place. The main role was played by the political apparatus the aim of which was to enhance its economic and political influence, and international interests. It can be concluded that the ownership of land in the Czech lands was always uneven. During the period, the state expropriated land from different society spheres and reallocated it to others. No other property in private ownership underwent so many ownership changes in the legal organization of the state. |
First record of the cottony cushion scale Icerya purchasi (Hemiptera, Monophlebidae) in Slovakia - short communicationShort CommunicationJán Kollár, Ladislav Bakay, Michal PástorPlant Protect. Sci., 2016, 52(3):217-219 | DOI: 10.17221/23/2016-PPS Damage by the cottony cushion scale Icerya purchasi (Hemiptera: Coccoidea: Monophlebidae: Iceryini) was found on Rosmarinus officinalis at the locality Suchohrad in Slovakia. Icerya purchasi is a cosmopolitan plant pest of warmer climates. In Central Europe it is a pest of glasshouses. It is the first observation of the cottony cushion scale (at least short-term) occurrence in the outdoor conditions in Slovakia. |
The potato minituber production from microtubers in aeroponic cultureOriginal PaperK. RykaczewskaPlant Soil Environ., 2016, 62(5):210-214 | DOI: 10.17221/686/2015-PSE Minituber production by traditional method is an expensive procedure due to limited productivity. In order to increase the multiplication rate of seed material in vitro, multiple techniques have been assayed in the last decades. The aeroponic system is a soilless culture system, where roots are kept in a dark environment saturated with aerosol of nutrient solution. Aeroponics technology is potentially efficient for specific potato cultivars. The aim of this study was to investigate the multiplication rate of microtubers of national cultivars in aeroponics while maintaining weekly intervals between harvests. The total number of harvests was 14 to 15 depending on year. The results of the experiments were analysed with ANOVA and means were separated with the Tukey's test at 5% P-value. The most important parameter of minituber production, their number, was on average 32.5-36.0 per plant and 1268-1396 per m2 depending on cultivar. Number of minitubers was two to three times greater in the case of aeroponic production than by traditional method. A full economic analysis is necessary to prove that this production technique can be put into practice. |
Soil phosphorus and potassium availability in long-term field experiments with organic and mineral fertilizationOriginal PaperM. Káą, G. Mühlbachová, H. Kusá, M. PechováPlant Soil Environ., 2016, 62(12):558-565 | DOI: 10.17221/534/2016-PSE The effect of organic, mineral and combined organic and mineral fertilization of soils on the winter wheat yields and nutrient contents in soils was evaluated in long-term field experiments. Two sites with different soil characteristics were evaluated - Lukavec u Pacova (cambisol) and Ivanovice na Hané (degraded chernozem). The type of fertilization influenced wheat yields. Nutrient uptake by winter wheat was higher under nitrogen (N) fertilization, which resulted in a negative balance of phosphorus (P) and potassium (K) and to a decrease of nutrient contents in the more fertile soils at Ivanovice na Hané. Two soil tests (Mehlich 3 method and NH4-acetate method) were used to determine P and K availability. The mineral nitrogen fertilization negatively and significantly affected NH4-acetate extractable concentrations of nutrients in the soils and these were lower in comparison with concentrations of P and K determined by Mehlich 3 method. Relative availability of P in alkaline soils from Ivanovice treated with mineral N increased while the soil pH decreased. |
Composition of cultivable enteric bacteria from the intestine of Antarctic fish (family Nototheniidae)Original PaperI. Sedláček, E. Staňková, P. ©vecCzech J. Anim. Sci., 2016, 61(3):127-132 | DOI: 10.17221/8785-CJAS Selenium (Se), copper (Cu), and zinc (Zn) play important antioxidant role during the transition period of dairy cattle. However, there is limited information about their blood fluctuations during the entire transition period, especially in heifers. Furthermore, it is questionable whether the use of clinoptilolite, a natural zeolite, affects the availability of these trace elements during this period. The objective of the present study was to monitor the blood concentrations of Se, Cu, and Zn during the transition period of dairy heifers and to investigate whether the dietary inclusion of clinoptilolite has any effect on them. Forty clinically healthy Holstein heifers were used in the experiment. They were randomly allocated in two equal groups (n = 20) formed according to their body condition score. The control group was fed only the basal ration whereas the daily feed of treatment group was supplemented with 200 g clinoptilolite. The experiment started 28 days before the expected day of calving and lasted until day 21 after parturition. Blood samples were taken at the onset of the experiment and then at weekly intervals until parturition, at the day of calving, and on days 7, 14, and 21. All samples were analyzed for blood Se and plasma Cu and Zn concentrations. The results indicate that the levels of Se, Cu, and Zn in blood change significantly (P < 0.05) throughout the transition period in dairy heifers and increase significantly (P < 0.05) immediately after calving. Furthermore, the dietary administration of clinoptilolite does not significantly affect their blood concentration (P > 0.05). Blood levels of Se, Cu, and Zn, although undergoing significant changes throughout the transition period in dairy heifers, remain practically stable until parturition and increase significantly immediately after calving. Clinoptilolite does not impair the dietary availability of the trace elements evaluated when added in heifers' rations during this period. |
INDEX OF VOLUME 62editorsRes. Agr. Eng., 2016, 62(4):X1 | DOI: 10.17221/9359-RAE |
Hyperostotic tympanic bone spicules in domestic and wild animal speciesOriginal PaperA. Heitmann, B. Parzefall, M. Zollner, A. Bruhschwein, W. Hermanns, A. BlutkeVet Med - Czech, 2016, 61(4):187-194 | DOI: 10.17221/8819-VETMED "Hyperostotic tympanic bone spicules" (HTBS), or "mucoperiosteal exostoses" (ME, syn.) are small, globular (≥ 1 mm in diameter), mostly stalked and drumstick-like, bony structures, which arise from the inner wall of the tympanic bulla and project into the middle ear cavity. HTBS present as mineral densities inside the tympanic bulla on radiographs or computed tomographic (CT) images. They have previously been referred to as "otoliths" and were thought to represent mineral concretions secondary to otitis media. Recently, it was shown that HTBS actually consist of regularly composed bone tissue, covered by normal middle ear mucosa. So far, HTBS have only extensively been described in dogs, where they occur with a prevalence of up to > 45%. A recent study detected ME, most likely representing HTBS, in the tympanic cavities of skeletonised skull bones of African lions. To estimate the occurrence of HTBS in other mammal species, the middle ears of adult animals of 78 different domestic, wild, and zoo species undergoing routine necropsy at the Institute of Veterinary Pathology of the LMU Munich, Germany were examined in the present study. HTBS were found in the tympanic bullae of carnivorous species, such as canids (wolf, fox), and in several large felid species (lion, tiger, leopard, cheetah). In contrast, HTBS were not present in domestic cats (more than to 200 cases), small carnivorous species such as mustelids, nor in any primate, ungulate, ruminant, pig, insectivore, or rodent species. The detectability of HTBS by CT of the tympanic bullae of large felids was demonstrated in an African lion. Histologically, HTBS consisted of mature lamellar bone, covered by periosteum and a partially ciliated, flat epithelium, regularly without any apparent inflammatory alterations. The present study demonstrates that HTBS may frequently occur in large felids and in different canid species. These findings should be taken into account when examining the middle ear, or interpreting bulla radiographs/CT-images of the respective species. However, the factors triggering the development of HTBS remain to be identified. |
Presence of Arcobacter species in pet cats and dogs in the Czech RepublicOriginal PaperM. Pejchalova, S. Zabcikova, L. Silhova, D. Silha, I. Brozkova, M. HaslovaVet Med - Czech, 2016, 61(8):449-455 | DOI: 10.17221/273/2015-VETMED This study was conducted to evaluate the occurrence of the genus Arcobacter in cats and dogs in the Czech Republic. These animals may be carriers of the bacteria and potential sources of human infection. Oral smears were collected from animals using smear swabs and brushes. Based on previous studies, commercially available DNA kits were used for DNA isolation. Samples were analysed using polymerase chain reaction (PCR) and evaluated using gel electrophoresis. Overall, 178 oral smears were tested, of which 108 were from dogs and 70 were from cats. Out of all smears, five were positive, of which four samples were from dogs and one from a cat. In all five positive cases, PCR confirmed the presence of Arcobacter butzleri. In follow-up sampling, the presence of Arcobacter butzleri was demonstrated in two samples from a dog. |
Assessing economic pressure on the forest fund of Maramureș County - RomaniaOriginal PaperC.C. Draghici, D. Peptenatu, A.G. Simion, R.D. Pintilii, D.C. Diaconu, C. Teodorescu, R.M. Papuc, A.M. Grigore, C.R. DobreaJ. For. Sci., 2016, 62(4):175-185 | DOI: 10.17221/72/2015-JFS Romania is also included among the European Union countries where deforested areas have radically increased in recent years, with mountain areas being the most affected. The pressure on the forest fund was analysed in the Maramureş County, as one of the most deforested counties of Romania. In view of assessing the forest area evolution, forested and deforested areas have been calculated for the period 2001-2012, using the Global Forest Change 2000-2012 database provided by the Department of Geographical Sciences, Maryland University. The economic pressure quantification was monitored by developing a database on economic activities based upon the loggings carried out in the period 2001-2012. This database comprises the number of companies, their turnover, profit and the number of employees for the economic sectors causing pressure on the forest fund. The outcomes show a dramatic increase in deforested areas, amounting to over 16,500 ha in the Maramureș County. Nearly 5,000 ha have been deforested in Borșa, the commune with the largest deforested areas in the period 2000-2012, causing major imbalances to the local ecosystem. |
Fermented buttermilk-based beverage: impact on young volunteers' health parametersFood Analysis, Food Quality and NutritionRaimondas Narkevičius, Arvydas Kaminskas, Jonas Algis Abaravičius, Dalius Vitkus, Valerija Jablonskienė, Dalia SekmokienėCzech J. Food Sci., 2016, 34(2):143-148 | DOI: 10.17221/344/2015-CJFS The technology of a functional fermented buttermilk-based beverage was developed and the impact of beverage consumption on the health parameters of young volunteers was studied. Three functional beverages were made on the basis of buttermilk, which differed in the composition. The beverage containing the buttermilk-skimmed milk-milk protein concentrate (0.3%) as a product with the preferred quality characteristics (synaeresis, viscosity, sensory properties, and acceptability) was used for further studies regarding the impact on human health. The medical nutrition experiments did not show any statistically significant effect on the biochemical blood parameters of 25 young volunteers after a 21-day period of the consumption of fermented buttermilk beverage with milk protein concentrate, although some of them (e.g. total, low- and high-density cholesterol, triacylglycerol concentrations, etc.) slightly decreased. The anthropometric and body composition, arterial blood pressure, and pulse evaluation indicators of recipients did not change significantly. |
Effect of calcium on reducing salt stress in seed germination and early growth stage of Festuca ovina L.Original PaperF. Salahshoor, F. KazemiPlant Soil Environ., 2016, 62(10):460-466 | DOI: 10.17221/319/2016-PSE Festuca ovina L. is an important ornamental species used in urban landscapes and pastures reclamation. However, low resistance of this species to salt stress has hindered its extended application in low-water and salty regions. To study the effects of calcium on reducing salt stress injuries in seed germination and early growth of this species, a factorial experiment based on completely randomized design with three replications was conducted. The salt levels included NaCl solutions (5, 10, 15 and 20 dS/m). Calcium source was CaCl2 with concentrations of 0, 10, 20 and 30 mmol+/L. Germination percentage and mean germination time were significantly affected by interaction of salinity and calcium. As salinity increased, mean germination time increased. However, germination percentage, shootlet length, rootlet length, shootlet fresh and dry weight, rootlet fresh and dry weight and seed vigour index decreased following the increasing salinity. Concentrations of 10 and 20 mmol+/L CaCl2 had significant effects on reducing salt injuries on seed germination of Festuca ovina. This finding was even prominent in higher salinity levels of 15 and 20 dS/m. Therefore, to facilitate using this species in urban landscapes or in pastures, pre-treatment of the seeds using 10 and 20 mmol+/L CaCl2 is recommended. |
Effect of dietary magnesium, calcium, phosphorus, and limestone grain size on productive performance and eggshell quality of hensOriginal PaperM. Skřivan, M. Englmaierová, M. Marounek, V. Skřivanová, T. Taubner, T. VítCzech J. Anim. Sci., 2016, 61(10):473-480 | DOI: 10.17221/3/2016-CJAS Two experiments conducted on laying hens, aged 50 and 52 weeks, were carried out to evaluate the effect of dietary Mg and its relationship to the Ca : NPP (non-phytate phosphorus) ratio using a various grain-size of limestone. The Ca : NPP ratio in each experiment was 12.8 and 18, respectively. Two Mg levels were evaluated in the first (1.56 and 4.0 g/kg) and the second experiment (1.52 and 4.0 g/kg). A fine-grained limestone (< 0.5 mm; FL) or a coarse-grained limestone (0.8-2 mm; CL) was used in the first experiment. In the second experiment, a mixture of both the aforementioned limestone forms (FCL) was used as the third alternative. The main parameters estimated in this study were egg production and egg shell breaking strength. In the first experiment, CL significantly increased hen-day egg production (P = 0.043) and Mg (in dietary concentration up to 4 g/kg) increased egg weight (P < 0.001). The addition of Mg to the mixed feed, together with CL, decreased yolk percentage (P = 0.008), increased egg shell percentage (P = 0.044), increased egg shell thickness (P = 0.014), and egg shell breaking strength (P = 0.003). Higher dietary Mg, together with a wider Ca : NPP ratio in the second experiment, increased egg production and egg weight (P < 0.001), but it did not influence egg shell breaking strength. CL increased egg shell breaking strength compared to the addition of FL, as well as FCL (P < 0.05), regardless of the Mg levels. Lower level of Mg with FL decreased ash content of shells (P = 0.004). |
Effects of dietary Enterococcus faecium on growth performance, carcass characteristics, faecal microbiota, and blood profile in broilersOriginal PaperM. Mohammadi Gheisar, A. Hosseindoust, I.H. KimVet Med - Czech, 2016, 61(1):28-34 | DOI: 10.17221/8680-VETMED This research was performed to evaluate the effect of supplementing broiler diets with a probiotic containing Enterococcus faecium on growth performance, carcass characteristics, faecal microbiota, and blood profile. A total of 384 one-day-old Ross 308 broiler chicks (mixed gender) with an average initial BW of 39.2 g were used in a 35 days feeding trial. The chicks were allotted to pens with 16 birds per pen and eight replications per treatment with food and water provided ad libitum. Treatments were: (1) basal diet, (2) 0.25% probiotic, and (3) 0.5% probiotic. Results indicated that body weight gain (BWG) on Day 7 to 21, Day 21 to 35 and overall (0 to 35) increased (P < 0.05) linearly but feed intake (FI) and feed conversion ratio (FCR) were not affected. A linear increase (P < 0.05) was observed in the relative weight of breast muscle when comparing the 0 to 0.5% concentration of probiotic, but breast meat colour was not affected by treatments. A significant impact (linear effect, P < 0.05) was observed on drip loss on Day 1. Inclusion of probiotic decreased (P < 0.05) the count of Salmonella linearly but the counts of E. coli and Lactobacillus were not affected. There was no remarkable influence on blood profile. Thus, it was concluded that inclusion of a probiotic containing Enterococcus faecium improved growth performance and altered the intestinal microbial population, without any negative effects on meat colour and blood profile in broiler chickens. |
Reversal of acute kidney injury after peritoneal dialysis in a dog: a case reportCase ReportP.T.C. Guimaraes-Okamoto, S.S. Geraldes, J.F.A. Ribeiro, A.N.L.S. Vieira, L.P. Porto, P. Barretti, M.L.G. Lourenco, A. MelchertVet Med - Czech, 2016, 61(7):399-403 | DOI: 10.17221/13/2016/VETMED Acute kidney injury is characterised by a sudden injury to the renal parenchyma and causes defects in its excretory, metabolic and endocrine function. Dialysis therapy has been instituted in small animal clinics with the aim of removing the metabolic waste and correcting the electrolyte disturbances stemming from renal dysfunction. Peritoneal dialysis is a therapy based on the use of the peritoneum as a semipermeable membrane through which solutes and fluids are exchanged between blood from the peritoneal capillaries and the dialysis solution. This report describes a case of acute kidney injury stemming from drug therapy in a 13-year-old female mongrel canine. The patient exhibited anorexia, emesis, prostration and anuria and had a history of prolonged treatment with meloxicam. The patient also presented with azotaemia and metabolic acidosis. When anuria continued to persist after drug therapy (volume restoration, chemical and osmotic diuresis and renal vasodilation), peritoneal dialysis was instituted. Three cycles of peritoneal dialysis were performed; during the second cycle, the patient's anuria was reversed, and at the end of the third cycle she showed a significant reduction in azotaemia, hyperkalaemia and an improved metabolic acidosis. Therefore, peritoneal dialysis showed satisfactory clinical results and reversed anuria, reduced azotaemia and electrolyte disturbances, thereby providing clinical improvement. |
Impact of climatic conditions on the reproductive phenological phases of European hazel (Corylus avellana L.) in SlovakiaOriginal PaperJ. ©kvareninováJ. For. Sci., 2016, 62(2):47-52 | DOI: 10.17221/55/2015-JFS The work presents the results of phenological observations of flowering and fruit ripeness of the European hazel (Corylus avellana L.) in Slovakia in two time periods: 1964-1983, and 1994-2013. The phenological stations at elevations from 100 to 875 m a.s.l. were grouped to 7 elevation zones, each 100 m wide. In the first studied period, flowering started on 19 March on average, while in the second period it started 5 days earlier. The average duration of flowering equal to 7-9 days did not depend on the elevation, but on the air temperature and weather patterns in the different elevation zones. During the first period, the average elevation gradient of flowering was 5.6 days per 100 elevation meters, while in the second period it was reduced to 3.5 days in the entire elevation profile. In the elevation zones between 300 and 500 m, a high correlation (P < 0.01) between the onset of flowering and elevation was reduced to a moderate level of correlation due to changing environmental conditions. In 1994-2013, the ripeness of hazel nuts started on average on 30 August in Slovakia, showed low variability and moderate correlation with elevation. The phenological elevation gradient was 2.9 days per 100 m of elevation. |
The use of products containing a phage in food industry as a new method for Listeria monocytogenes elimination from food (Listeria monocytogenes phages in food industry) - a reviewReviewDamian Pietracha, Anna MisiewiczCzech J. Food Sci., 2016, 34(1):1-8 | DOI: 10.17221/217/2015-CJFS Increased detection records of Listeria monocytogenes in foods have been recently observed. Standard methods of L. monocytogenes elimination from food products including pasteurisation or acidification may, however, be unreliable. The elimination of L. monocytogenes using specific bacteriophages which are natural antagonists of these pathogenic bacteria has become an innovative method that does not affect the taste, smell or appearance of the product. The use of phage preparations in food production is becoming an increasingly popular method for the effective and safe elimination of L. monocytogenes from food products. |
