Fulltext search in archive
Results 2491 to 2520 of 4551:
Assessment of risks in the field of safety, quality and environment in post-harvest lineOriginal PaperM. ®itňák, K. Kollárová, M. Macák, M. Prístavková, M. BoąanskýRes. Agr. Eng., 2015, 61(10):S26-S36 | DOI: 10.17221/23/2015-RAE The objective of this work is the evaluation of threats in the field of safety, fire safety, quality and environment in the post-harvest treatment of grain, with an emphasis put on pre-cleaning and drying of grain. The paper describes the technology of pre-cleaning and drying of grain. Risks for individual machines were evaluated using a failure mode and effects analysis (FMEA), which is used to evaluate the threats in terms of fire risk, occupational safety, the effect of machine on the quality of final product, and effect on the environment. We have proposed measures and solutions to eliminate or minimise the consequences of threats. Risk rates are evaluated before and after measures adopted. It can be stated based on the results that risk was successfully reduced to an acceptable level after adopting measures. |
Influence of different essential oils on refrigerated fish patties produced from bonito fish (Sarda sarda Bloch, 1793)Food Analysis, Food Quality and NutritionHusnu Sahan GURAN, Gulsum OKSUZTEPE, Ozlem Emir COBAN, Gokhan Kursad INCILICzech J. Food Sci., 2015, 33(1):37-44 | DOI: 10.17221/188/2014-CJFS The effects of different concentrations of thyme (group T), clove (group Cl), and rosemary (group R) extracts on the microbiological, chemical, and sensory attributes of fish patty made from the bonito fish (Sarda sarda Bloch, 1793) were investigated. The numbers of total mesophilic aerobic bacteria, coliform bacteria, Staphylococcus-Micrococcus spp. and yeasts and moulds in group R were lower than those of the other groups during storage (P < 0.05). During the storage period, total volatile basic nitrogen (TVB-N), thiobarbituric acid index (TBA-i), peroxide values, and free fatty acid content gradually increased in all groups, and significant differences were found between the groups (P < 0.05). The results of sensory evaluation showed that the shelf life of fish patties was 4 days for the control group, and 8, 10, and 14 days for the groups treated with thyme, clove and rosemary essential oil, respectively (P < 0.05). In conclusion, the addition of essential oils showed a positive effect on the product shelf-life; and in particular, rosemary essential oil produced a remarkable effect. |
Dihaploid induction in tetraploid durum wheat (Triticum durum L.) using pollen of Imperata cylindricaOriginal PaperHarinder Kumar CHAUDHARY, Anima MAHATO, Vineeta KAILA, Shoukat Ahmad RATHER, Tisu TAYENGCzech J. Genet. Plant Breed., 2015, 51(4):142-147 | DOI: 10.17221/218/2014-CJGPB Doubled haploidy breeding protocols have revolutionized the varietal development programmes in bread wheat, however, the protocols have not proved much efficient in durum wheat. Presently, the durum wheat × maize system is being widely followed for haploid induction but the frequency of haploid development is very poor which has limited its application in durum wheat improvement programmes. In order to formulate an efficient wide hybridization-mediated approach for haploid induction in durum wheat, different genotypes of durum wheat were subjected to hybridization with Imperata cylindrica, a wild perennial grass for the first time in this laboratory. The investigation was carried out for two seasons. During the 1st year, the investigation was carried out on one genotype only, Langdon, in order to notice the development of haploid embryos and the factors that influence the haploid induction frequency. The most important factor influencing the embryo formation frequency was found to be the concentration of 2,4-dichlorophenoxyacetic acid (2,4-D). Upon pollinating the emasculated spikes of durum wheat genotypes, various concentrations of 2,4-D were injected into the uppermost internode of wheat culm for three consecutive days after pollination to find out the most responding concentration for haploid induction. During the next year, the protocol was applied to eight durum wheat genotypes. The frequency of haploid induction parameters varied with the durum wheat genotypes as well as 2,4-D concentration used. The mean pseudoseed and embryo formation frequency over all the genotypes ranged from 30.2 to 56.3% and 1.2 to 18.4%, respectively. The average embryo formation frequency over all the genotypes was found to be highest (18.4%) at 250 mg/l 2,4-D whereas it was superior for the genotypes WH 896 and Langdon over all the 2,4-D concentrations. At the most responding 2,4-D concentration (250 mg/l), the genotype A-9-30-1 exhibited the highest embryo formation frequency (32.1%). The ploidy status of the developed embryos was identified using cytological analysis carried out on the rootlets of the tissue culture generated plantlets. |
Long-term effect of high phosphorus doses on zinc status of maize on a non-calcareous loamy soilOriginal PaperR. Kremper, G. Zsigrai, A.B. Kovács, J. LochPlant Soil Environ., 2015, 61(1):1-5 | DOI: 10.17221/509/2014-PSE The long-term effect of 87.3 kg/ha P on the yield elements and nutrient content of maize was studied at the National Long-Term Fertilization Experiment of the Karcag Research Institute in Hungary. The soil of the experiment site is non-calcareous Luvic Phaeosem, and its soluble phosphorus (P) and zinc (Zn) content in 0-20 cm soil layer are: ammonium lactate P: 141.1 mg/kg and diethylene triamine pentaacetic acid (DTPA)Zn: 0.85 mg/kg, respectively. The effect of foliar Zn fertilization was studied at three levels of nitrogen (150, 200 and 250 kg/ha) and under 87.3 kg/ha P and 82.6 kg/ha K application in four replications. The applied Zn amount was 700 g/ha. We measured the grain yield and the thousand-kernel weight. Leaf and grain samples were analyzed for phosphorus, zinc, potassium, calcium, magnesium and manganese content. Foliar Zn application did not increase the yield significantly, but it enhanced the thousand-kernel weight. The element content did not change significantly - neither in leaves nor in kernels. Under the examined habitat circumstances even the long-term application of 87.3 kg/ha P dosage did not cause Zn deficiency to such an extent which would lead to significant yield depression of maize. |
Potassium availability and soil extraction tests in agricultural soils with low exchangeable potassium contentOriginal PaperM. Madaras, M. KoubováPlant Soil Environ., 2015, 61(5):234-239 | DOI: 10.17221/171/2015-PSE Low application of fertilizer potassium (K) in intensive agriculture leads to a gradual decrease and, afterwards, to a steady state of soil K determined by tests which are based on soil exchangeable K (Kexch) extraction. In this situation, non-exchangeable K (Knon-exch) is being released and therefore real plant availability does not necessarily correspond to soil test K. This incoherency was investigated in 14 agricultural soils with low K as determined by the Mehlich 3 method. Plant-available K released by exhaustive cropping of perennial ryegrass and K determined by the Neubauer seedling test were compared with 8 soil K extraction methods, with the total K content and with the relative content of soil K-bearing minerals. K determined by the ryegrass test (314 mg/kg on average) was more than 3-times higher than K based on Kexch extraction methods, but was from 2 to 3 times lower than K determined by Knon-exch extracting methods. The relative content of mixed-layer phyllosilicates was significantly related to K extracted by soil tests. The relative content of orthoclase correlated only with total K and mica-group minerals with none of the extractions. The best prediction of plant-available K in investigated soils was obtained with sodium tetraphenylboron and StepK methods. |
The influence of mineral fertilization and legumes cultivation on the N2O soil emissionsOriginal PaperT. Sosulski, E. Szara, W. Stępień, B. RutkowskaPlant Soil Environ., 2015, 61(12):529-536 | DOI: 10.17221/229/2015-PSE The research aimed at the assessment of the influence of mineral nitrogen (CaNPK) fertilization and lupine cropping on the N2O emissions from agricultural soil. Observations were collected from CaNPK and Ca fertilization systems (further referred to as NIL due to the absence of nitrogen (N) fertilizers) in two consecutive years (2012 and 2013) on a long-term (since 1923) field experiment in Skierniewice in Central Poland. N2O emissions from the soil were measured in situ by the means of infrared spectroscopy using a portable FTIR spectrometer Alpha (Bruker). N2O fluxes from soils treated under CaNPK and NIL treatments were similar. No significant influence of the current treatment or cropping on the N2O emissions was noted in the CaNPK treated soil. N2O emissions in 2012 (barley, ammonium nitrate application) and 2013 (lupine, no mineral nitrogen application) were similar (0.17-23.04 g N2O-N/ha/day, median 4.29 and 0.09-19.46 g N2O-N/ha/day, median 4.45, respectively). During the growing period of 2012 (barley, ammonium nitrate application), the N2O-N emissions from the CaNPK treated soil (uncorrected for NIL) represented 1.02% of the applied N dose. In the growing period of 2013 (lupine, no mineral nitrogen application), the yield-scaled N2O-N emissions from CaNPK and NIL treatments equaled respectively to 4.4 g and 5.4 g N2O-N per 1 kg of nitrogen accumulated by lupine. |
Hydrothermal carbonization of stabilized sludge and meat and bone mealOriginal PaperJ. Malaȇk, T. DlabajaRes. Agr. Eng., 2015, 61(1):21-28 | DOI: 10.17221/59/2013-RAE Hydrothermal carbonization is one of suitable methods for energy recovery of sewage sludge and meat and bone meal. The task of the article is to determine appropriate hydrothermal carbonization process conditions and their impact on the quality of the final product - so called biochar or hydrochar. Parameters of the two main phases - initiation and polymerization - were monitored. The basic fuel properties of the final solid products of hydrothermal carbonization were determined. To produce biochar by hydrothermal carbonization, multifunctional pressure vessel with accessories was used - a batch reactor BR-300. Process parameters of hydrothermal carbonization confirm the effect of increasing temperature to increase the lower heating value (LHV). Neither calorific values of meat and bone meal (17.22 MJ/kg), nor calorific values of digested stabilized sludge (12.14 MJ/kg) showed a significant increase after undergoing processing. The effect of reaction temperature on the LHV of the final product is significantly higher than that of residence time. The results show that the main factor affecting LHV of the fuel sample is the final amount of ash. Unlike the meat and bone the hydrothermal carbonization of the stabilized wastewater sludge is one of the effective processing methods for subsequent energy use. |
Conditions conducive to an epidemic of Gremmeniella abietina, European race, in red pine plantationsOriginal PaperG. Laflamme, D. RiouxJ. For. Sci., 2015, 61(4):175-181 | DOI: 10.17221/67/2014-JFS In North America, Gremmeniella abietina, European race (GaEU), was reported in 1975. Our objective was to follow the spread of GaEU on red pines growing on flat land and on slope. Annual height infection varied significantly on flat land, ranging from 60 to 110 cm in 1991 to 0 to 50 cm in 1992. On the slope, pines in the bottom were killed by the disease, but survived on the top. Favorable conditions follow a horizontal line about 10 m over the lower elevation and are probably related to fog or mist. The horizontal disease spread over a 3-year period was only 20 m and this is mainly explained by the absence of ascospores in North America. |
Trade liberalization and state support of agriculture: effects for developing countriesScientific InformationVasily Erokhin, Anna Ivolga, Wim HeijmanAgric. Econ. - Czech, 2014, 60(11):524-537 | DOI: 10.17221/137/2013-AGRICECON The paper aims at the overview of the main approaches of state support implemented by developed and developing countries and the assessment of their distorting influences on the trade with agricultural commodities. The current state of state support is analyzed on the examples of EU-27, the USA and Japan. The experiences of developing countries are summarized on the examples of China, Russia and the CIS countries. State support tools are grouped according to the WTO classification. The alternative classification is implemented in order to compare the distorting effects caused by the state support. The EPACIS model is implemented to analyze the effects of trade liberalization for the distribution of agricultural commodities and trade policies in developing countries. The model is adjusted in order to assess the effects of state support for the production and trade with agricultural commodities in developing countries, recently accessed the WTO (the case of Russia). Imitation calculations allow formulating policies for the developing agricultural markets depending on the degrees of their sensitivity to foreign trade regulations and domestic support. |
Pea Fusarium wilt races in western AlgeriaOriginal PaperAoumria Merzoug, Lakhdar Belabid, Mokhtar Youcef-Benkada, Fouzia Benfreha, Bassam BayaaPlant Protect. Sci., 2014, 50(2):70-77 | DOI: 10.17221/20/2013-PPS The fungus Fusarium oxysporum f.sp. pisi (FOP), the pea wilt pathogen, causes appreciable yield losses under favourable environmental conditions in Algeria. Studies on the pathogen variability and distribution of races are essential to identify effective sources of resistance to this disease. In this study, a survey was conducted during the period 2007-2011 in four different agro-climatic zones. Pathogenic variability in 52 isolates of FOP, collected from different pea-growing areas of western Algeria, were evaluated using 7 genotypes as differential hosts. Results indicated that the disease was prevalent in all fields prospected and isolates obtained were assigned to Races 1, 2, 5 or 6 by their pathogenicity. It was found out that Races 1 and 2 were more common in all areas with 61.5 and 19.2%, respectively. This study is the first report of pea Fusarium wilt races distribution in Algeria. |
Seed transmissibility of viruses in winter squash landraces collected from the Black Sea region of TurkeyOriginal PaperMehmet A. SEVIK, Ahmet BALKAYAPlant Protect. Sci., 2015, 51(4):195-199 | DOI: 10.17221/6/2015-PPS The presence of seed-borne viruses in winter squash landraces in the Black Sea region of Turkey were detected. The seed samples of landraces were tested by ELISA for Cucumber mosaic virus (CMV), Zucchini yellow mosaic virus (ZYMV), Squash mosaic virus (SqMV), Tobacco ring spot virus (TRSV), and Cucumber green mottle mosaic virus (CGMMV). Based on the ELISA results, 11.3% of the samples were infected with the viruses; ELISA, CMV was found at the rate of 5.1% in the winter squash samples, while incidence of ZYMV was determined as 6.2%. Moreover, none of the plants germinated from seeds were found to be infected with SqMV, TRSV, and CGMMV. This is for the first time that seed-borne viruses of winter squash landraces have been identified in the Black Sea region of Turkey. |
Rapid detection of total nitrogen content in soy sauce using NIR spectroscopyFood Analysis, Food Quality and NutritionJie XU, Furong HUANG, Yuanpeng LI, Zhe CHEN, Yong WANGCzech J. Food Sci., 2015, 33(6):518-522 | DOI: 10.17221/229/2015-CJFS A method for the rapid and nondestructive determination of total nitrogen content in soy sauce was explored. Prediction models were established using near-infrared spectroscopy combined with each of the following techniques: partial least squares (PLS), interval PLS, synergy interval PLS (siPLS), and backward interval PLS. Results showed that each improved forecast model was better than the PLS model. The siPLS method exhibited the best performance. The full spectrum of light soy sauce was divided into 20 subintervals. The combination of four subintervals, namely, 6, 11, 13, and 18, showed the best effect, with a correlation coefficient of 0.9977 and an RMSECV of 0.0198. The full spectrum of dark soy sauce was divided into 20 subintervals. The combination of three subintervals, namely, 14, 17, and 19, showed the best effect, with a correlation coefficient of 0.9818 and an RMSECV of 0.0640. Therefore, the siPLS method can realise a rapid and accurate detection of total nitrogen content in soy sauce. |
Variability in macroelement content in the aboveground part of Helianthus tuberosus L. at different nitrogen fertilization levelsOriginal PaperB. Sawicka, D. Kalembasa, D. SkibaPlant Soil Environ., 2015, 61(4):158-163 | DOI: 10.17221/956/2014-PSE The contents of nitrogen (N), potassium, phosphorus, calcium, magnesium and sodium were estimated in tubers of Jerusalem artichoke Helianthus tuberosus coming from the field experiment conducted over 2010-2012. The experimental factors were the cultivars of Jerusalem artichoke cvs. Albik and Rubik and different nitrogen fertilization levels, against phosphorus and potassium fertilization and the full dose of manure. Determination of elements in the soil and the dry weight of the aerial parts are performed by standard methods. When using a fixed level of phosphorous-potassium fertilizer, the highest sodium content was obtained at a level of 50 kg N/ha, magnesium and sulphur at a dose of 100 kg of N, nitrogen - 150 kg N/ha, potassium and calcium - in the objects of fertilizer phosphorus-potassium, and phosphorus - in the building control without fertilization. Cv. Albik proved to be more abundant in mineral elements than cv. Rubik. The latter was characterized by a higher stability of characteristics. |
Production results of intensification of cultivation technologies in three lupin (Lupinus L.) speciesOriginal PaperM. Borowska, J. Prusinski, E. KaszkowiakPlant Soil Environ., 2015, 61(9):426-431 | DOI: 10.17221/455/2015-PSE The paper presents results of 3-year field studies carried out in a split-block design in four replications in the years 2012-2014 at the Agricultural Experiment Station in Mochełek (Poland). The effect of intensification of cultivation technologies on the yield of morphologically diversified cultivars of white (Lupinus albus), yellow (L. luteus) and narrow-leafed lupin (L. angustifolius) was examined. Traditional cultivars of white and yellow lupin gave significantly higher yields than the self-completing ones, as opposed to narrow-leafed lupin in which the self-completing cultivar had higher yields. Increasing expenses on industrial production means caused an increase in the yield of all the studied species. Seed yield of white and narrow-leafed lupin was significantly the highest in high-input technology, while that of yellow lupin in high- and medium-input technology. In all lupin species, insignificant diversification was observed in the number of pods per plant in medium- and high-input technologies as well as in low- and medium-input technologies. A significant increase in the seed yield along with an increasing intensity of the cultivation of traditional and self-completing cultivars of white and yellow lupin, as well as self-completing cultivars of narrow-leafed lupin, resulted mainly from developing a higher number of pods. |
Effect of replacing dietary FeSO4 with equal Fe-levelled iron glycine chelate on broiler chickensOriginal PaperR. Shi, D. Liu, J. Sun, Y. Jia, P. ZhangCzech J. Anim. Sci., 2015, 60(5):233-239 | DOI: 10.17221/8173-CJAS Iron (Fe) is an essential mineral for animal development and function. The present study was carried out to evaluate the effect of replacing FeSO4 with iron glycine chelate (Fe-Gly) in the equal Fe level in diets on broiler chickens. The broilers were randomly allotted to 6 dietary treatments with 5 replicate pens and 10 birds per pen. The treatments consisted of: Control group (100 mg Fe from FeSO4/kg diet), Experimental group 1 (80 mg Fe from FeSO4 + 20 mg Fe from Fe-Gly/kg diet), Experimental group 2 (60 mg Fe from FeSO4 + 40 mg Fe from Fe-Gly/kg diet), Experimental group 3 (40 mg Fe from FeSO4 + 60 mg Fe from Fe-Gly/kg diet), Experimental group 4 (20 mg Fe from FeSO4 + 80 mg Fe from Fe-Gly/kg diet), and Experimental group 5 (100 mg Fe from Fe-Gly/kg diet). The results showed that replacing FeSO4 with Fe-Gly in the diets did not significantly improve broiler growth performance (P > 0.05). But it significantly (P < 0.05) improved the blood biochemical parameters. Xanthione oxidase activity in blood serum showed no significant difference between all treatments at day 21 except for Experimental group 5 (P > 0.05). In addition, catalase activity in blood serum and Cu/Zn superoxide dismutase activity in liver were increased with the increasing replacement level of Fe-Gly (P < 0.05). But for all of the above indicators, the observed values of Experimental groups 3, 4, and 5 did not significantly differ (P > 0.05). This study indicates that replacing FeSO4 with Fe-Gly in the equal Fe level in the diets cannot improve the growth performance of broilers. But it can effectively improve the blood biochemical parameters and antioxidative enzyme activity. The least substitution ratio for low feeding cost and beneficial effect on the broilers was 60%. |
Penetrating eye injury in a dog: a case reportCase ReportM. Lew, S. Lew, M. Drazek, A. PomianowskiVet Med - Czech, 2015, 60(4):213-221 | DOI: 10.17221/8110-VETMED A four-year-old, male German Shepherd dog with severe pain in the left eye following a corneal perforation with a foreign body was examined. An ophthalmic examination revealed conjunctival hyperaemia and pancorneal dense oedema, preventing a diagnosis of deeper structures of the eye and lowered IOP. Vision testing was missing or impossible to detect. Ultrasonography showed a solid hyperechoic line protruding through the iris and lens into the vitreous and minor posterior lens displacement. The dog qualified for immediate surgical treatment. Intraoperative ophthalmic examination revealed a rupture of the anterior hyaloid membrane with vitreous herniation, posterior lens subluxation, lens capsule rupture and a torn iris. Partial iridectomy and intracapsular lens extraction (ICLE) was conducted. Slit-lamp biomicroscopy revealed iridodonesis and a gradual reduction of the corneal oedema, leading to complete transparency in the bottom two-thirds of the area on Day 12 after surgery. Direct and consensual PLR was sluggish and the dazzle reflex was positive. An electroretinographic examination confirmed normal activity of the retina. |
The influence of sex, age and season on the haematological profile of alpacas (Vicugna pacos) in Central EuropeOriginal PaperT. Husakova, L. Pavlata, A. Pechova, L. Tichy, K. HauptmanovaVet Med - Czech, 2015, 60(8):407-414 | DOI: 10.17221/8415-VETMED The aim of this study was to establish reference intervals for the haematological profile of alpacas on the basis of a large population of clinically healthy animals, and to determine the influence of sex, age and season on these indicators. Blood samples were collected from 243 alpacas (53 males and 156 females over six months of age and 34 crias - 12 males and 22 females - under six months of age). The selected farms were located in Central Europe (Czech Republic and Germany). We determined 13 haematological indicators. Comparison of the results was performed with respect to the sex of animals and for the older group also with regard to the season and to the feeding period. We found no highly significant (P > 0.001) differences between males and females. We did find highly significant differences (P < 0.001) between the group of crias under six months of age and the older alpacas (mean corpuscular volume - MCV, mean corpuscular haemoglobin concentration - MCHC, red cell distribution width - RDW, white blood cell count - WBC, neutrophil count). Based on our findings we suggest that for some indicators different reference intervals (esp. WBC and differential cell counts) be used for the two above mentioned age groups. We found some highly significant differences (P < 0.001) in haematological indicators in the older group of alpacas between the summer and winter feeding period (haemoglobin concentration, MCHC). Clinical laboratory diagnosis may be improved by the use of age-based and season-based haematological reference values. |
In vitro storage of plum germplasm by slow growthOriginal PaperS. Gianní, F. SottileHort. Sci. (Prague), 2015, 42(2):61-69 | DOI: 10.17221/186/2014-HORTSCI In this study, in vitro slow growth storage was investigated in four cultivars of two Sicilian (Southern Italy) plum species (Prunus domestica L. and Prunus cerasifera Ehrh. - two genotypes each). Established shoot cultures were preserved at 4°C in the dark in a Murashige and Skoog basal medium containing one of two different concentrations of sucrose (20 and 30 g/l) and with or without growth regulators. We tested the effects of cold storage, genotype and media on survival and re-growth capacity of explants after 3, 6, 9 and 12 months of storage. Effective minimal growth under cold conditions occurred in all four genotypes. The media composition did not affect survival, which, instead, appeared to be genotype-dependent. P. domestica genotypes survived cold storage the longest, for 12 and 9 months; instead, P. cerasifera ones remained viable for up to 6 months. All genotypes retained proliferation capacity under standard conditions and their re-growth capacity seems to be strongly genotype-dependent, closely related to their individual performance in response to the experimental condition of storage. |
Risk perception, attitudes towards risk and risk management: evidence and implicationsOriginal PaperErwin WAUTERS, Frankwin van WINSEN, Yann de MEY, Ludwig LAUWERSAgric. Econ. - Czech, 2014, 60(9):389-405 | DOI: 10.17221/176/2013-AGRICECON The comprehensive risk analysis of a business such as farming entails questions on what is at stake, how important is the risk concern and how to deal with it. We performed a sequential mixed method, with the in-depth interviews in the first stage (n = 35), followed by a survey on the Flemish FADN (n = 614) in the second, to investigate the farmers' risk perception, the attitudes towards risk and the perceived usefulness of the risk management strategies. We find that, rather than the short-term volatility in prices, the longer term co-evolution of expenses versus receipts is of a major concern to farmers, next to the land availability and the policy risks. Farmers are shown to be only slightly risk averse, rather risk neutral even. Further, our results suggest that farmers do not consider extensively studied risk management strategies such as contracts, futures and insurances, a valid option for their farm, and put more faith in internal strategies such as the debt management, the liquidity management and diversification. Last, risk management is to a substantial degree performed at the household level, rather than at the farm level, with strategies such as cutting the private expenses and the off-farm employment. These results hardly differ according to the farm and farmer characteristics. |
First report of Fusarium poae associated with and/or causing silvertop on loloid-type Festulolium in the Czech RepublicOriginal PaperJan NEDĚLNÍK, Miroslava STREJČKOVÁ, Tereza SABOLOVÁ, Bohumír CAGA©, Zdeněk BOTH, Jana PALICOVÁ, Bronislava HORTOVÁPlant Protect. Sci., 2015, 51(3):136-140 | DOI: 10.17221/19/2015-PPS Silvertop is a serious disease of grasses, and severe infestations cause a great deal of damage every year (particularly to seed producers). Inflorescences of infected plants dry prematurely and are sterile. The genera Festuca, Poa, Agrostis, Trisetum, and Cynosurus are the most frequent hosts of this disease, which has newly been reported in a loloid-type intergeneric Festulolium hybrid. The disease was also observed in the genus Lolium, with only rare previous description. The suspicion that Fusarium poae is the causal agent of severe silvertop was confirmed, and the pathogen was morphologically described and confirmed by molecular analysis. The meadow plant bug (Leptopterna dolabrata) may be a vector transferring the pathogen to plants, although a direct pathogen transfer was not demonstrated. Grass colonisation by the pathogen therefore apparently occurs after plant tissues injury by sucking of the meadow plant bug. |
Influence of Saccharomyces cerevisiae strain on the profile of volatile organic compounds of blossom honey meadFood Chemistry and SafetyIldikó Bénes, Katarína Furdíková, Daniela ©mogrovičováCzech J. Food Sci., 2015, 33(4):334-339 | DOI: 10.17221/48/2015-CJFS The influence of yeast strain on the volatile profile of meads fermented from blossom honey using three different Saccharomyces cerevisiae var. bayanus strains was evaluated. Meads were analysed by methods of basic chemical analysis and gas chromatography after 15 days of fermentation. Individual yeast strains produced various metabolites in different concentrations under the same fermentation conditions which significantly influenced the final secondary aroma of mead. Higher concentrations of acetaldehyde and 1-propanol, associated with S. cerevisiae var. bayanus MM-R2 considerably distinguished this strain from the others, whereas the difference between strains S. cerevisiae var. bayanus FM-R-Fix1 and MT-R1B was characterised by the production of ethyl hexanoate, ethyl octanoate (FM-R-Fix1), and isobutyl alcohol (MT-R1B). |
Antagonistic activity of selected fungi occurring in the soil after root chicory cultivationOriginal PaperE. Patkowska, M. Błażewicz-WoĽniak, M. KonopińskiPlant Soil Environ., 2015, 61(2):55-59 | DOI: 10.17221/920/2014-PSE The present studies determined the antagonistic activity of selected fungi of the soil environment of root chicory -a high-inulin plant, with considerable pro-health values - towards such fungi pathogenic towards this plant as: Altenaria alternata, Botrytis cinerea, Fusarium culmorum, F. oxysporum, Thanatephorus cucumeris and Sclerotinia sclerotiorum. The cultivation of root chicory took into consideration soil mulching with cover crops (oats, tansy phacelia and common vetch) as well as the conventional cultivation, i.e. without any cover crops. The total population of fungi after soil mulching with common vetch was almost twice as small as in the control and smaller than with phacelia as a cover plant. Antagonistic Clonostachys spp., Myrothecium spp., Penicillium spp. and Trichoderma spp. displayed differentiated activity towards the studied fungi. The greatest antagonistic effect was observed after the mulch of oats. Besides, oats and common vetch the most positive effect on the antagonistic activity was that of Clonostachys spp., Myrothecium spp., Penicillium spp. and Trichoderma spp. Those fungi were most effective in inhibiting the growth and development of F. oxysporum, T. cucumeris and S. sclerotiorum. |
Effect of orexin-A on prolactin secretion in lambs born under different photoperiod conditions - in vitro studyOriginal PaperE. Molik, M. Błasiak, T. Misztal, K. Romanowicz, D.A. Zięba-PrzybylskaCzech J. Anim. Sci., 2015, 60(1):33-37 | DOI: 10.17221/7909-CJAS The role of orexin-A in the regulation of prolactin secretion in lambs born in different photoperiods was determined. The experiment was conducted with 30 female lambs. Pituitary glands were collected from 40-day-old lambs in three different seasons (n = 10 per each season) and photoperiods (light (L) hours to darkness (D) hours): long (May - L 14 : D 10), intermediate (August - L 13 : D 11), and short (December - L 10 : D 14). At 40 days of age, the lambs were decapitated, and their pituitaries were dissected and cut in half along the longitudinal fissure so that each half contained both glandular and nervous parts. The in vitro incubation of the glands was performed for 3 h in Parker's medium at 37°C. In each season, the control pituitary glands (K1, K2, K3) were incubated in Parker's medium, while the experimental pituitary glands (Ox1, Ox2, Ox3) were incubated in medium containing 1000 ng/ml of exogenous orexin. The administration of orexin-A during the long photoperiod (May) caused a significant (P ≤ 0.01) increase in prolactin secretion during the first hour of incubation (Ox1: 47.56 ± 8.4 ng/ml vs K1: 36.08 ± 7.8 ng/ml). During the intermediate photoperiod (August), a significantly (P ≤ 0.01) higher concentration of prolactin was observed in the first hour of incubation in the Ox2 group than in the control group (K2). During the first hour of incubation in December, the prolactin concentrations were significantly (P ≤ 0.01) higher in the Ox3 group than in the K3 group. Orexin-A increased prolactin secretion from pituitary explants collected from lambs born under all investigated time-points. The study results indicate that orexin-A is a stimulatory factor of prolactin secretion in sheep. |
Computed tomography-guided cementoplasty combined with radiation therapy for an aneurysmal bone cyst in a dog: a case reportCase ReportM. Vignoli, L. Stehlik, R. Terragni, L. Cavallo, P. ProksVet Med - Czech, 2015, 60(2):109-114 | DOI: 10.17221/7986-VETMED Aneurysmal bone cysts are expansile osteolytic bone lesions that can manifest clinically as lameness, pain and swelling. In humans, aneurysmal bone cysts are considered benign tumours that have been classified as primary or secondary to a previous coexisting lesion. Local recurrence after treatment or malignant transformation has been reported in both humans and in a dog. Different treatment options have been proposed. This report describes the treatment of an 11-month-old Irish wolfhound with an aneurysmal bone cyst with a combination of computed tomography-guided cementoplasty and radiation therapy, 30 Gy delivered in 10 fractions. The dog recovered completely, with no lameness observed 30 months after treatment. |
Collie eye anomaly: a reviewReviewA. PalanovaVet Med - Czech, 2015, 60(7):345-350 | DOI: 10.17221/8381-VETMED Collie eye anomaly (CEA) is an inherited congenital visual impairment with heterogeneous signs. The first symptoms are already visible in the early embryo. Among the most affected breeds are Collies and Shetland Sheepdogs but the disease has spread to different breeds depending on the country of origin. Dogs affected with this disease share a 7.8 kb deletion in intron 4 of the NHEJ1 gene. Inheritance of this disease is autosomal recessive with incomplete penetrance. Thanks to a commercially available genetic test breeders can identify genetically affected recessive homozygotes and clinically healthy but genetic carriers of the mutation and thus select healthy parents for the next generation of dogs. However, the exact cause of the disease is not known and it is not known whether the causative mutation influences the occurrence of some other diseases (e.g. immunodeficiencies). |
Evaluation of effect of supplementation of extruded rice as a substitute for dried whey in the diet of weanling pigsOriginal PaperS. Mohana Devi, S.C. Kim, I.H. KimVet Med - Czech, 2015, 60(12):675-682 | DOI: 10.17221/8581-VETMED A total of 120 weanling pigs [(Landrace × Yorkshire) × Duroc], 21 days of age with an average initial body weight (BW) of 6.52 ± 0.22 (SE) kg were selected to investigate the effects of extruded rice product as a replacement for dried whey on growth performance, coefficient of total tract apparent digestibility (CATTD), blood profiles, faecal shedding of Lactobacillus and Escherichia coli (the microbial counts of digesta were expressed as log10 colony forming units per gram), and faecal scores of weanling pigs. Pigs were randomly allocated to one of four dietary treatments, with six replicates per treatment and five pigs per pen. Pigs in the control group were fed a diet based on corn, soybean, and 20% dried whey. Experimental groups received the same diet as the control group, but dried whey was replaced by 3%, 6%, and 9% extruded rice. Throughout the experimental period (six weeks), no differences were observed in the average daily gain, average daily feed intake, and gain/feed ratio. Pigs fed 6% and 9% extruded rice had a lower (P < 0.05) CATTD than pigs fed the control diet on Day 14. The blood creatinine concentration of pigs fed 9% extruded rice was higher (P < 0.05) than that of pigs in the control group on Day 14. Pigs fed the diets containing 6% and 9% extruded rice had decreased (P < 0.05) faecal E. coli counts on Day 14 compared with pigs fed the control diet. The current results indicate that feeding extruded rice can decrease faecal E. coli counts without negative effects on growth performance in weanling pigs. |
Influence of material solution of cover shells on stable daylightOriginal PaperM. Balková, D. PáleąRes. Agr. Eng., 2015, 61(10):S67-S73 | DOI: 10.17221/47/2015-RAE The impact of material solution of cover shells on stable daylight. Light is supplied to the stable via a roof skylight, where wired glass is the infill of skylight structure, through open side walls, whereby the influx of light on one side is influenced by the shelter, and through the open gates, which are located in the front walls of the stable were assessed. Measurements of light intensity inside the stable were carried out in two height levels. Simultaneously, measurements were performed outside, on non-shadowed plane. Daylight factor was calculated from the measured values of internal and external comparative illuminance. Resulting values were processed into tables and graphs. The roof skylight has clearly the greatest influence on the lighting of the stable. Its disadvantage is that the stable overheats in these places in summer. Here, it would be appropriate to replace the fill of the skylight with translucent light elements. |
Stem rust resistance in 1BL.1RS and 2RL.2BS double wheat-rye translocation linesOriginal PaperMahbubjon RAHMATOV, Larisa GARKAVA-GUSTAVSSON, Ruth WANYERA, Brian STEFFENSON, Matthew ROUSE, Eva JOHANSSONCzech J. Genet. Plant Breed., 2015, 51(4):148-154 | DOI: 10.17221/80/2015-CJGPB The wheat stem rust pathogen, Puccinia graminis f.sp. tritici, is a significant and devastating disease of wheat crops worldwide. Wheat has many wild relatives in which to source new resistance genes, including the cereal crop of rye in the tertiary genepool. The aim of this study was to assess the reaction of 1BL.1RS and 2RL.2BS double wheat-rye translocation lines to virulent stem rust races from Africa and North America. BC1F3 and BC1F4 populations from a cross between the line KR99-139 (a double wheat-rye translocation line with 1BL.1RS and 2RL.2BS) and the bread wheat cultivar Topper were used in the study. Several of the populations homozygous for 1BL.1RS and heterozygous for 2RL.2BS showed resistance and low severity adult plant resistance (20RMR-50MSS) to the African stem rust race TTKSK in the field. None of the tested populations with varying chromosome combinations showed seedling resistance to any of the tested stem rust races. Thus, these resistant populations likely carry gene/s effective at the adult plant stage since all stage resistance genes with major effect appear to be absent based on the seedling assays. Resistant lines combined three chromosomes (1RS, 2RS and 2BS) which make their direct use in breeding more complicated. Mapping studies followed by potential transfer of genes between 2R and 2B will make the identified minor genes more useful in wheat breeding. |
Effect of Orychophragmus violaceus incorporation on nitrogen uptake in succeeding maizeOriginal PaperL. Yang, W. Cao, K. Thorup-Kristensen, J. Bai, S. Gao, D. ChangPlant Soil Environ., 2015, 61(6):260-265 | DOI: 10.17221/178/2015-PSE Winter Orychophragmus violaceus (OV)/spring maize is a novel eco-agricultural system in North China Plain, but little is known about OV's nitrogen (N) effects on succeeding maize growth and its contribution to maize N uptake and utilization during the growth. A pot experiment using 15N was conducted, including five treatments: (1) Control - without OV incorporation and N fertilizer; (2) G - high 15N labelled-OV incorporation; (3) 50%G - lower 15N labelled-OV incorporation; (4) F - 15N labelled urea fertilizer merely; (5) G + F - high OV incorporation combined with urea fertilizer. Increasing OV incorporation rate increased maize dry matter, yield, and total N uptake. Approximately 84-97% of the released OV's nitrogen was absorbed before the V8 (the 8th leaf fully expanded) stage in G and 50%G treatments. However, only 19% of the released OV's nitrogen was taken up at this period in G + F, and the rest was absorbed from V8 to maturity. G treated maize doubled the OV's nitrogen uptake than the 50%G at maturity, and also had higher percentage contribution of OV's N to total N accumulation. The G and 50%G maize obtained similar OV's nitrogen use efficiency of 30%; however, this efficiency was further improved to 43% in the G + F. And more, G + F treatment further improved the maize yield by 9% compared to F treatments. |
Evaluation of the mercury content of loamy sand soil after long-term nitrogen and potassium fertilizationOriginal PaperB. Rutkowska, B. Murawska, E. Spychaj-Fabisiak, Sz. Różański, W. Szulc, A. PiekutPlant Soil Environ., 2015, 61(12):537-543 | DOI: 10.17221/584/2015-PSE The study was based on soil samples taken after the long-term fertilizer experiment (1974-2014) where different doses of nitrogen and potassium were applied. The experiment was located at the Research Station of the UTP University of Science and Technology in Bydgoszcz (Poland). The long-term application of nitrogen and potassium fertilizers leads to changes in the concentration of mercury, soil acidification, reduction in total organic carbon, total nitrogen as well as affluence of available nutrients (P, K, Mg) and increased mobility of copper and zinc. The significant positive correlation between total mercury content in the soil and the content of N-NO3, Zn, N-NH4 and the hydrolytic acidity value were stated. |
