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Cranial cruciate ligament rupture (CrCLR) is a common stifle joint pathology among dogs, leading to osteoarthritis and painfulness. Non-surgical treatment options often represent the usage of non-steroidal anti-inflammatory drugs for 14 days (NSAIDs), but autologous conditioned plasma (ACP) shows promising results in managing various orthopaedic conditions, decreasing inflammation, and improving the clinical outcome in dogs. This study aimed to determine the differences in MMP-3 serum levels and the clinical outcomes between differently treated cranial cruciate rupture cases. For this purpose, we used two different treatment methods for managing canine cranial cruciate ligament rupture (minimally invasive ACP injection or oral NSAIDs), and evaluated the clinical outcomes, indicating the quality of life, and the MMP-3 serum levels over a period of 28 days. The findings of this investigation indicate that ACP has better efficacy than two weeks of NSAIDs in inflammation reduction, clinical outcome improvement, and the allowance of a longer duration of activity after 28 days.

Donkey milk is a very promising matrix for the isolation of new potential starter cultures with probiotic properties. We isolated, identified, and compared the technological properties of the donkey milk isolate D23 with those of goat milk isolates G15 and G17 and bovine milk isolates C3 and C9. All isolates were identified as Lacticaseibacillus paracasei using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF-MS) and polymerase chain reaction (PCR) assays (determination of species-specific DNA fragments). Like the bovine and goat isolates, the donkey milk isolate D23 was able to grow in De Man–Rogosa–Sharpe (MRS) broth at various temperatures (10, 25, 30, 37, and 45 °C) and at different NaCl concentrations (0–6.5% w/v). Additionally, D23 showed notable proteolytic and autolytic activity, could grow in and acidifying ultra high temperature (UHT) bovine milk but exhibited very weak diacetyl production. None of the isolates displayed hemolytic activity, nor produced histamine, fenylethylamine and cadaverine. Finally, isolate D23 demonstrated interesting antibacterial and antifungal properties compared to the goat and bovine isolates, especially against staphylococcus aureus CCM 3953.

Since its introduction in 2019, African swine fever (ASF) has spread to all regions of the Philippines, affecting 73 out of its 82 provinces. To assess the environmental DNA (eDNA) contamination patterns of the ASF virus (ASFV) in swine transport vehicles and evaluate its measures of association, a total of 450 environmental swabs from 30 transportation vehicles were tested using qPCR. Five out of 30 vehicles (16.67%) tested positive in at least one of the following areas: cargo area or sidecar walls (6.67%), cargo area or sidecar floors (6.67%), hauling personnel’s hands (6.67%), steering wheel or handlebars (3.33%), gear shift levers (3.33%), floor mats or footpegs (3.33%), dashboards (3.33%), door handles or sidecar gate bolts (3.33%), tyres/wheels (3.33%), fenders (3.33%), and hauling personnel’s footwear (3.33%). All investigated risk factors were analysed using Chi-square, Fisher’s exact test, and odds ratio and were found to be insignificant, including the frequency of swine transportation per week, frequency of cleaning per week, cleaning materials used, disinfection practices, the number of pigs transported, and whether hauliers owned pigs (p > 0.05). This study illuminates the environmental contamination patterns of ASFV in swine transport vehicles, underscoring the need for targeted biosecurity protocols in areas with a high risk of contamination, particularly the cabin areas. More effective vehicle disinfection systems that will not damage equipment or pose health risks to hauliers are recommended to reduce the risk of ASF disease transmission between countries and geographic regions.

The production of food of plant origin is critically dependent on the pollination ability of honey bees, whose health has been deteriorating for a long time, and whose population is declining. In our in vivo experiment on a honey bee brood at the 4-day larval stage, we tested the following concentrations of oxalic acid: 0% (control – applied distilled water), 0.87%, 1.75%, 3.5% and 7%, corresponding to doses of 0 mg, 2.61 mg, 5.25 mg, 10.5 mg, and 21 mg of oxalic acid per dm² of honeycomb with the brood. The LC₅₀ values (72 h) ranged between 3.17% and 3.33%. The different LC₅₀ values obtained resulted from three different methods used to calculate this indicator. The therapeutic index (TI) of oxalic acid was set to be 1.1, indicating a high risk to the honey bee brood. We observed an increased gene expression for the detoxifying enzyme glutathione-S-transferase (GST), but did not detect an increased gene expression for superoxide dismutase (SOD1 and SOD2), which protects the organism from oxidative stress. A decrease in gene expression was observed for prophenoloxidase and hymenoptaecin, while defensin and lysozyme did not show significant changes. These results emphasise the need for the accurate dosage and application of oxalic acid in the treatment of varroosis.

The substantial increase in grain production stimulated by traditional agricultural direct subsidies has been accompanied by a concomitant decrease in ecological quality, precipitated by excessive application of chemical fertilisers, which has generated countervailing effects that fundamentally undermine the positive effect of subsidy policies on agricultural output. Consequently, the mitigation of agricultural pollution and the elevation of ecological quality have emerged as pivotal directions for the reform of agricultural subsidies. Using both time-varying difference-in-differences (DID) models and spatial DID models in this study, we examined the effect of agricultural 'three subsidies' reform on agricultural fertiliser nonpoint source pollution (AFNSP), drawing on China's province-sector panel data from 2008 to 2022. The empirical evidence yields several salient findings. First, the three subsidies reform can significantly reduce AFNSP and improve ecological quality. Second, the large-scale operation of agricultural households and the enhancement of agricultural production efficiency serve as effective pathways for the three subsidies reform to reduce AFNSP. Third, the implementation of the three subsidies reform engenders significant spatial spillover effects, which play a crucial role in reducing overall regional AFNSP. Fourth, the efficacy of the three subsidies reform exhibits heterogeneity across diverse agroecological contexts and farming cultures. Last, the reform has resulted in notable improvements in agricultural ecological quality, thereby reinforcing food security capabilities. These findings not only offer valuable reference for refining agricultural subsidy reform but also contribute to the development of a comprehensive framework that simultaneously safeguards agroecological security and food security.

Ascochyta blight, caused by a complex of pathogenic fungi including Didymella pinodes, Ascochyta pisi, and Phoma pinodella, is a major disease of field pea (Pisum sativum), causing severe losses through lesions on leaves, stems, and pods. Mutation breeding using gamma irradiation is a non-GMO strategy to induce genetic variation and accelerate the development of improved genotypes. In this study, the M₂ generation of the forage pea cultivar Dodoni (Pisum sativum L. var. arvense), derived from M₀ seeds irradiated with 100 Gy, was evaluated for tolerance to D. pinodes (CBS 251.47) using a detached-leaf assay under controlled greenhouse conditions. Disease progression was quantified via image-based analysis on the 3^rd and 5^th days post-infection, calculating diseased area and disease severity index. Extensive phenotypic evaluation was also conducted on 16 families in the greenhouse and 100 families under field conditions, using an augmented incomplete block design. Screening revealed several M₂ families with significantly improved tolerance compared to non-irradiated controls. Among these, some individuals combined enhanced resistance with improved yield-related traits, such as higher pod number and biomass, while others exhibited reduced agronomic performance. These findings highlight the phenotypic diversity induced by gamma irradiation and demonstrate the potential to generate dual-purpose pea genotypes with both disease resistance and enhanced productivity, providing valuable material for future breeding of resilient cultivars.

Non-steroidal anti-inflammatory drugs (NSAIDs) are a cornerstone in the management of canine osteoarthritis (OA), despite concerns regarding their long-term safety. Among non-pharmacological alternatives, pulsed electromagnetic field (PEMF) therapy has gained attention for its potential analgesic and anti-inflammatory effects, although veterinary-specific evidence remains limited. This randomised, controlled pilot study compared the clinical efficacy of PEMF therapy versus NSAID treatment (Mavacoxib) in 16 dogs with clinically and radiographically confirmed OA. Dogs were randomly assigned to receive either a 12-session PEMF protocol over 45 days or a standard Mavacoxib regimen. Clinical evaluations included pain scores (VAS), pain on palpation, lameness severity (NRS), gait analysis (GLS and TPI), muscle circumference, and radiographic progression. Assessments were performed at baseline (T0), 30 days (T1), and 60 days (T2). PEMF therapy showed earlier improvements in pain, lameness, and muscle mass, and a favourable trend in joint mobility. No significant differences were detected in gait or radiographic parameters between groups. These preliminary findings suggest that PEMF is non-inferior to NSAIDs and highlight its potential role in multimodal OA management. Limitations include a small sample size and a short follow-up. Further studies are needed to confirm these preliminary results in larger cohorts.

This review examines bacteriophages as alternatives to antibiotic growth promoters (AGPs) in broiler chickens, with a unique focus on effects observed in studies without experimental bacterial challenges. Driven by global antimicrobial resistance (AMR) concerns and sustainable poultry production demands, phage supplementation, a targeted strategy, potentially improves growth performance and gut health by preserving beneficial microbiota. This literature analysis assesses phage efficacy in healthy broilers under non-challenge conditions, evaluating key variables such as phage concentration, delivery, and targets, as well as outcomes such as feed conversion ratio (FCR) and gut health markers. Crucially, this review extends beyond efficacy to explore phage immunomodulatory capabilities, outlines optimisation strategies, and addresses risks and mitigation. Key findings show mixed efficacy of phages in non-challenged broilers: significant improvements in FCR and gut health were generally observed with high phage concentrations (e.g., ≥10⁸ PFU/g) and continuous delivery protocols, whereas lower doses yielded inconsistent or minimal benefits. Furthermore, choosing which bacteria phages target – for example, aiming at pathogens like Salmonella or managing common gut bacteria such as E. coli – greatly impacts outcomes. While phages show considerable potential as AGP alternatives, the review highlights that further research is vital to resolve inconsistencies, standardise protocols, and understand host genetic and environmental influences to optimise their commercial application.

Organic seed coating is an alternative method for supporting sustainable agriculture. This study investigates the influence of organic coating and seed storability on cucumber seeds using atmosphere-controlling techniques. The seeds were coated with an organic formulation, and a non-coated seed was used as a control. All samples were then packed using modified atmosphere packaging, including normal air, 100% N₂, and 100% CO₂, and stored under ambient conditions (30 ± 2 °C) for 8 months. Results indicated that the organic seed coating did not significantly affect seed germination compared to the non-coated seeds (P > 0.05) throughout the storage period. Moreover, the coating tended to positively influence seedling growth, including root and shoot lengths, seedling growth rate, chlorophyll content, and total phenolic content. Additionally, seeds packed with 100% CO₂ showed a slight impact on seedling growth compared to those in normal airbags, but this modified atmosphere packaging technique tended to increase chlorophyll a and b, as well as the total phenolic content in seedlings. Conversely, seeds packaged with 100% N₂ tended to decrease seedling lengths. Therefore, cucumber seeds coated with an organic formulation and packed in a 100% CO₂ bag can enhance seedling growth parameters during germination and extend seed storability.

The aim of the study was to find out whether and how the forest soil compaction resulting from the traffic of forest logging machines results in the increased soil air concentration of CO₂, occurring over a longer period of time and in different seasons of the year. Changes in the soil air CO₂ concentrations were monitored in two periods: in winter (cold period) and in summer (warm period). CO₂ concentrations were measured in compacted and non-compacted soil using a certified measurement. In addition to the soil concentration of CO₂, air temperature, soil temperature and soil moisture content were measured. The research was conducted in the Czech Republic. The obtained data was subjected to statistical analyses (Student's t-test; correlation analysis). The results of the study confirm the long-term influence of soil compaction by the traffic of forest machinery on the CO₂ concentration in soil in both seasons (cold and warm). The concentration of CO₂ in the air of compacted soil was always significantly higher in both periods than the CO₂ concentration in the air of non-compacted soil (control). Thus, the negative influence of soil compaction was clearly demonstrated as a result of a single pass of forestry machines over the soil surface.

The aim of this study was to investigate the physiological characteristics and quality of hypocotyls in the production of selenium-enriched sprouts from peanut seeds soaked in selenium (Se) solution. Peanut seeds were soaked with 0, 2.5, 5.0, 7.5, and 10 μmol/L Na₂SeO₃ for 12 h and then germinated. The results showed that the selenium concentration in peanut shoots increased with increasing levels of selenite soaking, and there existed a crossroads of selenite soaking concentration (5.0 μmol/L) when selenium concentrations in cotyledons and hypocotyls were equal. Below and above this concentration, Se concentrations in shoots were radicle > cotyledon > hypocotyl or cotyledon > radicle > hypocotyl, respectively. In addition, Se significantly promoted the elongation of hypocotyls and radicles, increased shoot biomass, increased the activity of antioxidant enzymes and the concentration of antioxidants in hypocotyls, and decreased malondialdehyde levels. Moreover, Se significantly increased the concentrations of soluble sugars, proteins, free amino acids and resveratrol in hypocotyls. These results indicate that soaking peanut seeds with selenite significantly increased Se concentration, biomass, antioxidant capacity and quality of peanut shoots. This study provides a theoretical basis for the rapid and standardised production of Se-enriched peanut shoots from selenite-soaked seeds.

Snails, which are high in protein, have the potential to be developed as a flavour enhancer through the hydrolysis process. However, consumer acceptance of the flavour enhancer needs to be fully evaluated. The aim of this study was to determine the consumer acceptance of a snail protein hydrolysate from different snail species (golden apple, apple, and freshwater) and at different hydrolysis durations (3, 6, and 9 h), and to identify the drivers of liking of snail protein hydrolysates through descriptive profiling using Rate-All-That-Apply (RATA) method and consumer testing using Hedonic test. The RATA intensity data were subjected to analysis using analysis of variance, followed by a Tukey's post hoc test (P < 0.05). Furthermore, the sensory profile data were analysed using principal component analysis and preference mapping. Snail species and hydrolysis time influenced the sensory profile of snail protein hydrolysate, with the longer hydrolysis time being the most liked. The most liked flavour enhancer derived from golden apple snail with 9 h hydrolysis time had a strong savoury aroma, salty taste, umami taste, lingering mouthfeel, and yellow colour. Additionally, it exhibited a moderate intensity of seafood aroma, a garlic taste, an umami aftertaste, a liquid mouthfeel, and a salty aftertaste. However, it had a low intensity of bitter aftertaste and burnt taste, and a very low intensity of sweet aroma, sweet taste, bitter taste, and bland taste. Thus, these findings highlight the importance of evaluating the efficacy of flavour enhancers and facilitate the identification of the optimal snail species and hydrolysis time according to consumer preference.

Soil compaction has negative impacts on plant productivity. Degradation of soil structure as a result of soil compaction can inhibit the development of plant roots and make it difficult for plants to take up water and nutrients. This can negatively affect plant growth and productivity. Compaction restricts plant root growth by increasing mechanical resistance, reducing oxygen uptake, and thus reducing crop yields as it inhibits plant development. In this study, the effects of soil compaction due to machinery traffic on the physical structure of soil, morphological characteristics and yield of spinach were investigated in Tokat, where vegetable farming is intensively practised. In the study, four different tractor traffic treatments [C – zero tractor traffic (control), T1 – 1 tractor traffic, T3 – 3 tractor traffic, T5 – 5 tractor traffic] were used. As traffic increased, the penetration resistance, bulk density, and porosity increased, and the lowest volume weight was obtained from the control treatment. Plant weight losses in T1, T3 and T5 treatments compared to the control treatment were 1.92%, 31.09% and 64.64%, respectively. The yield value, which was 62.0 t/ha in the C treatment, was determined to be 31.8 t/ha in the T5 treatment, representing a 48.70% decrease. The proper use of modern agricultural machinery plays an important role in preventing soil compaction and increasing plant productivity.

Countries are calling for a sustainable transition of agri-food systems due to the volatility of food security in the context of climate change, and the agricultural bioeconomy may be a more dependable solution. By capturing, coding, and displaying word clouds of Chinese agricultural bioeconomy policy texts, this study deconstructs the development framework of China's agricultural bioeconomy policies based on the agricultural biosystem dimension, the policy instrument dimension, and the comprehensive dimension. It concludes that there is a clear trend of sustainable transformation and cross-sectoral linkage in China's agricultural bioeconomy policies. Overall, China's agricultural bioeconomy policies have the following comprehensive features: first, the policy is deeply integrated, considering both economic development and environmental friendliness; second, the policy is safety-oriented, taking into account competitiveness and strategy; and third, the policy is innovation-driven, taking into account fundamentality and foresight. Efforts should be made in the future to enhance the competitiveness of the agricultural bioeconomy within the framework of an all-encompassing approach to food and to help China's agricultural bioeconomy on the road to sustainable transition by improving fiscal and tax support, advocating the use of financial instruments, creating an alliance for the agricultural bioeconomy, and promoting international trade exchanges and cooperation.

Digitisation enables the integration of science, technology and finance, and this trend toward integration can in turn enhance the innovation efficiency of listed agricultural enterprises. By leveraging the quasi-natural experiment of China's 2011 pilot program for technology finance – also known as science, technology, and finance, this paper uses the difference-in-differences (DID) model to demonstrate that the science and technology finance policy positively impacts the innovation levels of agriculture-related listed enterprises. That said, the policy's effect varies across different regions and enterprise sizes. Furthermore, moderating-effect analysis reveals that the digitalisation level of agriculture-related listed enterprises is a critical factor, while the evolutionary game model elucidates this factor's underlying mechanism. Based on these findings, policy recommendations are proposed to further develop science and technology finance platforms for agricultural-related listed enterprises.

Agricultural carbon emissions (ACE) is a critical contributor to global greenhouse gas emissions, which have already become a common challenge for global carbon reduction. As a major agricultural producer and largest carbon emitter, China has made great efforts to reduce ACE. Using the panel data of 30 provinces in China from 2011 to 2022, this study explores the heterogeneous impacts of formal environmental regulations (FER) and informal environmental regulations (IER) on ACE. The results reveal that both FER and IER have significant effect on reducing ACE, with FER showing a more pronounced effect. The mechanism analysis indicates that agricultural technological innovation and planting structure adjustment play important mediating roles in this impact mechanism. The effect of FER is more remarkable in major grain producing areas than in non-major grain producing areas, while the effect of IER is completely opposite. Compared with coastal regions, both FER and IER have significant inhibitory effect on ACE in inland regions. Additionally, the marketisation level may reinforce the inhibitory effect of both FER and IER on ACE. Based on the empirical results, this study suggests to strengthen the synergistic effect of FER and IER, promote agricultural technology innovation, and formulate targeted policies according to regional differences.

The black locust (Robinia pseudoacacia L.) is the second most planted tree species worldwide, and the most common in Hungary. Phenotypic traits, particularly flowering patterns, are well-established indicators of the species' response to climate change. This study examined four forest subcompartments across three Hungarian regions: Northern-Central, Eastern and Southern-Central. The aim was to identify climatic factors correlating with the onset and duration of the flowering period. Additionally, the relationships between these factors and nectar weight and sugar concentration were defined. Results indicate a strong negative correlation between precipitation levels and flowering time: lower accumulated and average precipitation during the spring months of the preceding year was associated with a delayed flowering period in the following year (r = –0.922, r = –0.918, P = 0.05). However, when examining the 14-day period (r = 0.829) before blooming or examining from 1 January (r = 0.929, r = 0.890), the results indicate that other environmental factors may play a more dominant role. Furthermore, the number of chill and heat days was found to affect the starting date (R² = 0.819, R² = 0.765).

This study investigates the effects of digital economy (DE) on agricultural land use (ALU) in seven sub-Saharan African (SSA) countries, specifically from 2006 to 2022. Using a moderating mediation model on panel data, the work explores the extent to which the DE, as proxied by fixed telephone subscriptions, internet usage, and mobile penetration, influences the degree and intensity of ALU. The results indicate that ALU is often supported by DE, as technology will lay the groundwork for improved land management and agriculture. Results show that DE has a positive influence on ALU, with a more substantial effect being observed in countries such as Kenya and South Africa, where more developed digital infrastructure and governance are in place. In contrast, Uganda and Zambia exhibit lower impacts due to lower levels of digitalisation and governance barriers. The patent applications (PAs) and water management represent the positive mediators of the efficiency of land-use improvement. The study highlights the need for government-enabling policies and digital infrastructure so that the promise of digital technologies and their uptake for agricultural production in SSA is fully fulfilled. Whenever technology is integrated with an appropriate resource management policy, SSA societies have the potential to achieve sustainable agricultural development and food security.

Thawed meat is usually of lower quality and less expensive than chilled meat, which some dishonest people may exploit by fraudulently marketing it as chilled. This study focused on methods for detecting and distinguishing frozen meat from chilled meat. The activity of the enzyme aconitase in the eluate was determined, and the mineral cations (Na⁺, K⁺, Mg²⁺) as well as the concentrations of organic acids (acetic, citric, and lactic) were analysed in chilled and thawed meat stored for 7, 14, and 150 days at −18 °C. After 150 days of storage, aconitase activity increased from 47.2 ± 7.2 U·L^–1 to 395.3 ± 59.2 U·L^–1 in pork, from 45.8 ± 11.5 U·L^–1 to 133.3 ± 31.8 U·L^–1 in beef, and from 17.2 ± 8.6 U·L^–1 to 143.6 ± 41.5 U·L^–1 in chicken. The mineral content decreased during storage in meat samples, especially Na⁺ and K⁺ cations (P < 0.05). The results for organic acids were less conclusive. Principal component analysis (PCA) of the data confirmed a clear separation between chilled and thawed meat for all species, with a high variability of nearly 72%.

Salinity represents a significant abiotic stress that markedly influences plant growth through osmotic stress induction. Plants commonly undergo osmotic adaptation when subjected to prolonged periods of saline stress. The current experiments were conducted on five wheat (Triticum aestivum L.) genotypes with contrasting salt tolerance capacities – Mirbashir 128, Gobustan, Gyzyl bughda, Fatima, and Zirva 80 under salinity stress caused by 150 mmol NaCl. The relative water content and osmotic potential were found to decrease significantly in salinity-sensitive genotypes (Fatima and Zirva 80) compared to salinity-tolerant ones (Mirbashir 128, Gobustan, and Gyzyl bughda) when treated with 150 mmol NaCl. Salinity also caused the accumulation of soluble sugars and proline, the amounts of which were observed to be higher in salinity-tolerant genotypes than sensitive ones, while lipid peroxidation was higher in salinity-sensitive genotypes. In salinity-tolerant genotypes, 150 mmol NaCl caused increased antioxidant enzyme activities and accumulation of flavonoids, including anthocyanins, confirming the rapid development of the stress reactions in these plants. Differences in the osmoregulation indicators and antioxidant responses between salinity-tolerant and sensitive plants are assumed to be related to their salinity-tolerance traits. This investigation provides pivotal foundational insights for enhancing the salt tolerance of wheat genotypes, thereby potentially enhancing both yield and quality in diverse wheat cultivars thriving in saline environments.

The near-expired food (NEF) is a significant opportunity to reduce food waste, while consumers often associate NEF with safety issues, which results in a large amount of safe and healthy food being wasted globally. This research focuses on food date labelling (FDL) and explores how consumers' label cognition impacts their willingness to purchase NEF. Using a random sampling method online, we obtain 2 113 valid samples from China and conduct an information intervention 'quasi-natural experiment' to obtain participants' FDL cognition and willingness to purchase the near-expired milk (NEM) before and after the intervention and evaluate the impact of the intervention through the differences-in-differences model. The results show that consumers' initial purchase willingness for NEM is low, and their FDL cognition has a positive effect, especially in Eastern China and higher education consumers. Information intervention increases consumers' willingness to purchase NEM by changing their label cognition, and the intervention has a more pronounced impact among older, male, and higher education consumers. Considering the pressure on resources and the environment caused by food waste has become an impediment to sustainable development, the findings expand the application of the Knowledge, Attitude, and Practice (K-A-P) theory in the NEF field and clearly reveal the important role of eliminating consumer prejudice of FDL in reducing food waste to achieve the United Nations Sustainable Development Goal 12.3 'Halve food waste.'

Dragon trees, arborescent members of the genus Dracaena (Asparagaceae), provide a wide range of ecosystem services and have been ethnobotanically important plants since ancient times. Currently, their relictual distribution is fragmented, populations are isolated and often under threat. We provide a brief overview of scientific studies and the state of knowledge on dragon trees published since 2020, when the last review was published. More than 120 papers dealing with dragon trees have appeared, indicating a significant interest in their research, cultural uses and conservation. The most intensively investigated species are Dracaena cochinchinensis, D. cinnabari, D. draco and D. cambodiana. Almost half of the papers deal with the chemical composition of resin and its bioactivity, in addition to studies on ecology, conservation and genetics. Only a few studies are devoted to taxonomy and ethnobotany.

Foot diseases are one of the leading health problems that lead to significant yield losses in small ruminant breeding. This study aimed to clinically evaluate foot diseases in sheep and goats reared in Siirt province of Türkiye and its surrounding districts. Molecular methods were used to investigate the presence of Dichelobacter nodosus serogroups and Treponema spp. phylogroups in cases with identified lesions. Clinical examination of 4 111 sheep and goats identified foot diseases in 402 animals, affecting a total of 410 feet. Contagious ovine digital dermatitis (CODD) and digital dermatitis (DD) cases were identified in 66.82% and 26.82% of diseased feet, respectively. Footrot lesions were found in 4.87% of the feet in which the disease was identified. D. nodosus was detected in 66.23% of swab samples collected from 77 CODD cases, whereas Treponema spp. was identified in 2.59% of the samples. Among DD cases (n = 110), D. nodosus was found in 35.45% and Treponema spp. in 17.27% (Group 1 = 1.81%, Group 2 = 15.45%). The majority of D. nodosus strains identified in the cases (n = 90) were classified as serogroup A (37.77%) and serogroup D (60.00%). This study revealed that CODD is a major problem in small ruminant breeding in the Siirt province and its districts. It was determined that D. nodosus serogroup A, D and Treponema spp. Group 2 strains played an important role in the aetiology of foot diseases in sheep and goats. This study represents the first comprehensive investigation of foot diseases in sheep and goats in the Siirt province and marks a significant milestone as the first study in Türkiye to identify and analyse the aetiology causes of CODD in the literature.

The aim of this study was to analyse the conformation traits and body dimensions of Haflinger mares registered in the Czech Republic studbook. The study focused on the effects of age at studbook entry, year of birth, proportion of Arabian genes, and country of origin on various parameters. Data were collected from 825 mares, including scores obtained during the studbook entry evaluation and body measurements such as withers height measured with a stick (WHS), withers height measured with a tape (WHT), chest girth (CG), and cannon bone circumference (CBC). Based on these data, the bone index (BI) and body massiveness index (MI) were calculated. Statistical analysis was performed using multifactor analysis of variance (ANOVA) and Scheffe’s post-hoc testing method. The results showed that the age at studbook entry significantly affected conformation scores, chest girth, cannon bone circumference, and both indices (MI, BI). Mares aged 3–4 years had significantly higher conformation scores compared to mares aged 5–6 years (P = 0.001) and mares aged 7 years and older (P = 0.000). Mares aged 5–6 years and those aged 7 years and older showed higher CG values (P = 0.002, P = 0.000) and CBC values (P = 0.010, P = 0.001). Furthermore, the year of birth had a highly significant impact on all variables studied. The withers height of mares born between 2011 and 2020 was, on average, 3.49 cm higher (144.84 cm) than that of mares born between 1987 and 2000 (141.35 cm) (P < 0.000). Regarding CG, a statistically significant increase was observed in mares born in 1987–2000 and 2001–2010, with chest girth increasing from 180.45 cm to 183.76 cm (P = 0.016). However, a subsequent decrease to 179.91 cm was noted in mares born between 2011 and 2020 (P = 0.002). The influence of the proportion of Arabian genes was significant for WHS, where mares with a higher proportion of Arabian genes (>1.56%) showed lower WHS (141.74 cm) compared to mares with a lower proportion (143.86 cm) or no Arabian genes (143.01 cm). While ANOVA demonstrated a significant effect of Arabian genes on CBC, Scheffe’s post-hoc test did not confirm this finding. No statistically significant differences were observed in other body dimensions based on the proportion of Arabian genes. The influence of the country of origin was statistically significant for all variables except WHS and WHT. Regarding the conformation scores, mares from the Czech Republic scored lower (6.98) than Austrian mares (7.22, P = 0.000). Additionally, CG was significantly lower in Austrian mares (178.58 cm) compared to Czech mares (182.54 cm, P = 0.000). Austrian mares also had significantly lower MI (P = 0.000) and BI (P = 0.040), with Austrian indices at 124.71/13.31 and Czech indices at 127.77/13.41. Based on this empirical evaluation of the current and original population of Haflingers in the Czech Republic, the connections and influence of individual factors during breeding are revealed. The results of our analysis have built an objective scientific basis and they allow the breeding organisation to determine the further breeding process.

This study was conducted to determine the effects of phosphite and phosphate fertilisers on broccoli’s yield, growth, and nutritional status (Brassica oleracea L. var. italica cv. ‘Fiorentino’). In factorial combinations, experiments were conducted at three pH levels (5.5, 7.0, and 8.5) and three phosphorus source levels [Pi (phosphate), Phi (phosphite), and Pi + Phi]. Phi had a statistically significant negative effect on yield, producing immature and button-like flower heads. Decreases in chlorophyll index, Fv/Fm [ratio of variable fluorescence (Fv) to maximum fluorescence (Fm)], and leaf area were observed using the Phi treatment. These results indicate that fertilisation with Phi alone did not affect plant growth and yield. P (phosphorus) and K (potassium) concentrations in plants were increased by root fertilisation with Phi (floating system), but did not affect growth characteristics; increased Pi uptake in Phi-treated plants increased P and K concentrations in the sink source, resulting in reduced growth, phytotoxicity, and no head formation. While it may appear that Phi is upsetting the balance of solution nutrients and stressing the plants, the Phi-induced stress condition was identified by measuring proline levels and electrolyte leakage. Thus, phosphite could not be used as a P source for plants. However, Phi could be used as a plant nutrient source combined with conventional Pi fertilisers.

The purpose of this study was to detect the prevalence rate of European rose chafer (Cetonia aurata) on American highbush blueberries (Vaccinium corymbosum). The observation was made from the beginning of flowering to the end of harvest of the blueberry cultivars ‘Duke’, ‘Legacy’, and ‘Huron’. The incidence occurs during the first harvest of the ‘Duke’ cultivar. Three rows per cultivar, with 90 plants per row, were monitored during the research. Insects were collected on 15 infested plants per row, randomly selected, and counted. This procedure was repeated every second day, 3 times a day: in the morning, at noon, and in the afternoon. The highest presence of the insect was recorded at the full ripening phase of the ‘Duke’ cultivar, with 25.5% of bushes being infested and an average of 12.7 beetles per bush counted. The damage percentage varied over the harvesting periods: 29% of the fruits were damaged in the first harvest, 13% in the second harvest, and 8% during the third harvest. While ‘Legacy’ and ‘Huron’ stood out with 5.55% and 3.33% of infected bushes, respectively, and minor fruit damage. This research proves that the European rose chafer in the area of the experiment is a harmful pest of early cultivated blueberry cultivars.

This study investigates the benefits of using mine tailings (MT) to improve pea (Pisum sativum L.) growth and productivity on degraded agricultural soils in semi-arid environments. The research aims to evaluate the use of MT as an innovative soil amendment and to determine the optimal dose required to enhance the micronutrient availability of Zn, Mn, Cu and Fe without affecting soil quality. The experiment was conducted in greenhouse pots with three different soil types amended with different MT doses (control and four doses). Soil samples were collected from the Doukkala region, one of the main agricultural areas in Morocco. Pea was grown in pots and monitored for 87 days until maturity. After harvest, soil and plant samples were weighed, measured and analysed by inductively coupled plasma atomic emission spectroscopy (ICP-AES). The experiment found that moderate doses (0.2 g/kg to 1 g/kg) applied to all soil types promoted optimal pea growth by improving plant height, root and above-ground biomass and pod number. Thus, MT can act as a biostimulant. However, nutrient antagonism negatively affected growth at the highest dose (4 g/kg). Bioconcentration and translocation factors indicated efficient micronutrient uptake and biofortification, while heavy metals remained immobilised in roots, effectively eliminating toxicity risks.

This study investigated how wheat (C3) and maize (C4) respond to soil thallium (Tl) contamination and elevated CO₂ (eCO₂), aiming to understand strategies for mitigating oxidative stress. Under eCO₂, both crops showed higher biomass production. However, high Tl concentration (120 mg/kg) significantly decreased fresh and dry weights by 31–59%, which translated directly to compromised yield. This growth decline is linked to impaired photosynthesis, evidenced by a 54–57% drop in net photosynthetic rate under elevated Tl. Such photosynthetic inhibition intensifies oxidative stress, marked by increased membrane damage and hydrogen peroxide (H2O2). Furthermore, photorespiration contributed to oxidative stress by generating H₂O₂, with increased activities of glycolate oxidase and hydroxypyruvate reductase rising by 122% and 201%, in wheat and by 179% and 39% in maize, respectively, in response to 120 mg/kg TI under eCO₂ conditions. Simultaneously, to mitigate oxidative damage, antioxidant defences were significantly enhanced, resulting in increased activity of the ascorbate (ASC)/glutathione (GSH) cycle, along with elevated levels of metallothionein and phytochelatin for Tl sequestration, as well as augmented glutathione S-transferase activity. Overall, findings reveal complex interactions between CO₂ and Tl, highlighting species-specific adaptive responses of C3 and C4 plants. C3 plants use photorespiration to combat oxidative stress, while C3 and C4 plants have strong antioxidant systems to reduce the effects of oxidative stress, promoting crop resilience and growth despite Tl toxicity.

This study examines the prevalence of helminthic infestations, risk factors, and odds ratios in 1 300 cattle, revealing significant patterns in parasite distribution and influencing factors. Overall, 60.3% of cattle were infested with one or more types of parasites, with Moniezia spp. being the most prevalent (46.9%), followed by Fasciola spp. (36.9%), Paramphistomum spp. (26.8%), and Avitellina spp. (10.8%). Among the seasons, winter exhibited the highest infestation rate (66.1%), and calves under one year of age were more commonly infested (64.2%) than older cattle (over three years: 51.1%, OR = 0.584 1). Distinct seasonal and age-related patterns were observed for specific parasites. Fasciola spp. was most prevalent in winter (45.09%) and among the youngest cattle (47.87%), while Paramphistomum spp. and Moniezia spp. showed less variation across seasons. Avitellina spp. had the lowest infestation rates in the spring, and cattle older than three years were affected. Co-infections were common, notably between Fasciola and other parasites, with the highest co-infestation rate observed between Avitellina spp. and Moniezia spp. Analysis of deworming efficacy indicated higher treatment success for Fasciola spp. and Paramphistomum spp., lower odds of response for Avitellina spp., and no significant treatment effect for Moniezia spp. Microscopic and pathological examinations were also conducted. These results demonstrate the necessity of thorough deworming procedures along with targeted parasite control to reduce significant health hazards in cattle populations.

Heavy metal stress inhibits plant growth, but this impact is less studied and pronounced under climate change conditions. The present study investigates the physiological, biochemical, and agronomic responses of wheat (C3) and maize (C4) exposed to varying thallium (Tl) stress (60 and 120 mg/kg) under ambient (aCO₂) and elevated (eCO₂, 710 µmol/mol) CO₂ levels. High Tl exposure markedly reduced grain yield by 58% in wheat and 68% in maize at 120 mg/kg under aCO₂. However, eCO₂ partially offset the negative effects, increasing yield by ~20% in wheat and 36% in maize at 60 mg/kg Tl. eCO₂ enhanced photosynthetic activity under eCO₂, which increased the accumulation of soluble sugars under TI stress. These provide carbon skeletons for the synthesis of primary metabolites such as amino acids, organic acids and fatty acids. Although total fatty acid content declined under stress, the metabolic crosstalk initiated by improved photosynthesis and sugar availability enables plants to maintain key fatty acids (such as palmitic, linolenic, and oleic acids) essential for membrane stability and function. Amino acids, especially proline and cysteine, accumulated significantly under Tl stress. These primary metabolites, in turn, feed into secondary metabolic pathways, promoting the formation of phenolic acids and flavonoids that enhance antioxidant defence and stress tolerance. This metabolic cascade explains eCO₂’s capacity to alleviate TI stress and improve crop performance, and underscores the value of leveraging eCO₂ environments to support agricultural productivity and food security under challenging conditions.