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Effect of initial height of seedlings on the growth of planting material of Norway spruce (Picea abies [L.] Karst.) in mountain conditionsA. Jurásek, J. Leugner, J. MartincováJ. For. Sci., 2009, 55(3):112-118 | DOI: 10.17221/97/2008-JFS Common ways of nursery cultivation and sorting the planting material of mountain provenances of Norway spruce (Picea abies [L.] Karst.) are connected with the risk of undesirable narrowing of the genetic spectrum of populations. Investigations in spruce plantations established by different planting materials found out very good growth (total height is 125 cm 9 years after outplanting) and health status of these slowly growing seedlings planted in extreme mountain conditions. In order to prevent the genetic spectrum narrowing, we recommend to cultivate all seedlings including smaller outsorted (commonly culled) ones. The smallest seedlings can be grown one year longer and subsequently planted out in the same locality as the remaining planting material of the same seed lot. |
Agricultural and food wholesale and retail trade in the intra-community tradeM. ZábojAgric. Econ. - Czech, 2008, 54(9):419-430 | DOI: 10.17221/2701-AGRICECON The paper focuses on the evaluation of the contemporary situation of two economic sectors in the frame of the European Union, namely the wholesale and retail of agricultural and food products. For these industries, the structural profile with the indicators of turnover, value added and employment was elaborated. Hereafter the costs, productivity and profitability indicators ranking of the top 5 Member States were compared to the EU-25 averages. After a 3.1% increase in the turnover index in 2000, the evolution of sales for the agricultural wholesaling sector recorded a series of small increases and decreases in the turnover, ranging from - 0.6% in 2002 to 1.5% in 2004. Between 2000 and 2005, the agricultural wholesaling sector generally recorded a slower rate of expansion for turnover than the wholesale trade average. The specialised in-store food retailing sector generated EUR 120.4 billion of turnover in 2003. The wealth creation of the specialised in-store food retailing was valued at EUR 23.8 billion; equivalent to 6.5% of the retail trade and repair total, while this activity employed 1.4 million persons, some 9.0% of the retail trade and repair workforce. Half (49.9%) of the turnover in the specialised in-store food retailing sector in the EU was generated by enterprises selling fruit, vegetables, meat, fish or bakery products. |
New approaches to the solving of economic crisis in business managementE. SvobodaAgric. Econ. - Czech, 2009, 55(7):307-313 | DOI: 10.17221/50/2009-AGRICECON The paper presents the results of the research focused on crisis management, which is based on the process of change management by the author-formulated methodology of the creation, implementation and changes of business strategies and requirements for their improvement due to the incidence of a turbulent management environment and other essential factors. The main factors of crisis and methods of their analysis are formulated. Thereinafter, the strategies of solving crisis situations are formulated. The company Bioveta, Inc. is analysed in detail. The research has proved that companies with modern management need a well-made and working system of crisis management due to their complexity and risks. |
Peronospora hariotii on Buddleja in the Czech RepublicIvana ©afránková, Jiří MüllerPlant Protect. Sci., 2009, 45(1):12-15 | DOI: 10.17221/44/2008-PPS A downy mildew was observed on Buddleja × weyeriana Weyer ex Rehd. (B. davidii × globosa) cv. Sungold in a nursery in the northern part of the Czech Republic in July 2008. This downy mildew disease of Buddleja (Butterfly Bush) had been unknown in the country so far. Characteristics of visual symptoms and microscopic features are described. The morphology of the fungus was typical for Peronospora hariotii, conforming to the published description by GÄUMANN (1919). The sexual stage was not found. This is the first report of Peronospora hariotii causing leaf spot disease on Buddleja in the Czech Republic. |
Changes of Vitamin C Content in Relation to the Range of Accumulation of Cd, Pb and Zn in Potato TubersJ. Musilová, J. Bystrická, J. Tomáą, Z. Poláková, S. MelicháčováCzech J. Food Sci., 2009, 27(10):S192-S194 | DOI: 10.17221/614-CJFS The changes of vitamin C content in relation to Cd, Pb and Zn accumulation in 6 potatoes varieties: very early (Junior, Impala), early (Livera), middle early (Agria), medium-late (Asterix, Desirée) were surveyed in this work. The soil used in pot trial had pseudototal (in soil extract of aqua regia |
Effect of Lactococcus sp. on the growth of Listeria sp. in the model UHT milk systemEva ©VIRÁKOVÁ, Ivana SLO®ILOVÁ, Petr TICHOVSKÝ, Milada PlockováCzech J. Food Sci., 2009, 27(11):SII8-SII11 | DOI: 10.17221/672-CJFS The work was aimed at the growth suppression of cultured listerias strains by cultured lactococci strains or commercial mesophilic cheese cultures during common cultivations in the model UHT milk system (0.5% w/w of milk fat content) at 30°C during 18 h aerobically. Milk was primarily fermented by lactococci at the level of 108 CFU/ml and secondarily contaminated by listerias at the level of 103 CFU/ml. The most intensive growth suppressions of both Listeria innocua (CCM 5884 or Ln-03) strains were caused by Lactococcus lactis subsp. lactis (LCC 416 or CHCC 2281) strains or DELVO-ADD® 100-X DSF cheese culture; the listerias growth reductions was from the level of 103 CFU/ml to 100 CFU/ml. Obtained results should be applied to dairy industry provided that HACCP, GHP and GMP systems must be observed. |
Influence of arbuscular mycorrhizal fungi on uptake of Zn and P by two contrasting rice genotypesR. Hajiboland, N. Aliasgharzad, R. BarzegharPlant Soil Environ., 2009, 55(3):93-100 | DOI: 10.17221/319-PSE There is little experimental evidence about the functional significance of arbuscular mycorrhizal fungi (AMF) colonization in providing nutrients for lowland rice. This study was undertaken to examine whether growth and nutrient deficiencies may affect plants benefit from AMF inoculation. Two contrasting rice (Oryza sativa L.) genotypes and two AMF species (Glomus mosseae and G. intraradices) were used in this experiment. Under P starvation, P uptake in the genotype tolerant to P deficiency (Fajr), declined significantly up to 36% (P < 0.05) in response to AMF inoculation, while it enhanced by about 70% (P < 0.01) in susceptible genotype (Shafagh). Under Zn starvation, Zn uptake of Zn-efficient genotype (Shafagh) increased by about 2 fold (P < 0.01), but a reduction of 52% (P < 0.05) was observed in the Zn-inefficient genotype (Fajr) upon mycorrhization. Greater genotypic differences were observed for -P-Zn plants. Our results imply that genotypic difference in responsiveness to inoculation with AMF is attributable to different contribution of mechanisms for increased nutrient uptake in mycorrhizal plants depending on nutrient, nutritional status and nutrient efficiency of genotypes. |
Soil micromorphology use for modeling of a non-equilibrium water and solute movementR. KodeąováPlant Soil Environ., 2009, 55(10):424-428 | DOI: 10.17221/137/2009-PSE Soil micromorphology was applied to specify flow domains in different soils and to select a suitable numerical model for simulation of water flow and herbicide transport. Pore structure detected on soil micromorphological images represented in all cases domains of prevailing water flow and solute transport. Depending on pore configuration and boundary conditions either water immobilization or preferential flow was observed and simulated. The benefits and limitations of the soil micromorphology imaging are discussed and compared with the more often used X-ray computer tomography, magnetic resonance imaging and dye tracer imaging. |
Effects of grass species on ruminal degradability of silages and prediction of dry matter effective degradabilityF. Jančík, V. Koukolová, P. Kubelková, B. ČermákCzech J. Anim. Sci., 2009, 54(7):315-323 | DOI: 10.17221/1725-CJAS Forty samples of grass silages, made from the five most widely used grass species in ruminant nutrition (Dactylis glomerata L.,Phleum pratense L., Lolium perenne L., Festuca arundinacea L. and hybrid Felina) were tested in the present study. These grass species conserved by an ensiling process were compared among each other according to dry matter (DM) rumen degradability parameters (a = portion of DM solubilized at initiation of incubation, b = fraction of DM potentially degradable in the rumen, c = rate constant of disappearance of fraction b and EDDM = effective degradability of DM, estimated for each ingredient assuming the rumen solid outflow rates of 0.02 (EDDM2), 0.05 (EDDM5) and 0.08 (EDDM8) h-1). Based on the chemical composition of grass silages the regression equations for prediction of EDDM were evaluated. The influence of the ensiling process on dry matter degradability parameters was also assessed. The best values of EDDM were determined for Lolium perenne (EDDM2 = 753.2, EDDM5 = 631.1 and EDDM8 = 567.7 g/kg DM). The best predictor was NDF (R2-values of 0.757 (EDDM2), 0.863 (EDDM5) and 0.906 (EDDM8)). Using two predictors the accuracy level increased. The combination of CF and NDF gave R2-values 0.892, 0.920 and 0.929 for EDDM2, EDDM5 and EDDM8, respectively. The regression equations based on the most important grass species harvested in different vegetation periods seem to be a useful tool for practical use. No significant P < 0.05) effect of the ensiling process in relation to dry matter rumen degradability parameters was proved. |
Effects of moisture content, loading rate, and grain orientation on fracture resistance of barley grainH. Tavakoli, S.S. Mohtasebi, A. Rajabipour, M. TavakoliRes. Agr. Eng., 2009, 55(3):85-93 | DOI: 10.17221/6/2009-RAE Force and deformation curves of agricultural materials must be provided for the proper design of harvesting and processing machineries. In this research, the fracture resistance of barley grain was measured in terms of the grain rupture force and energy. In this study, 8 treatments were performed as a randomised complete block design with 10 replications. The barley grains were quasi-statically loaded in horizontal and vertical orientations with the moisture content at four levels: 7.34, 12.11, 16.82, and 21.58% dry basis, and loading rates at two levels: 5 and 10 mm/min. Based on the results obtained, the force required for initiating the grain rupture decreased from 161.97 to 93.94 N, and from 75.37 to 50.16 N, and the energy absorbed at the grain rupture increased from 36.42 to 74.70 mJ and from 27.34 to 62.65 mJ, for horizontal and vertical orientations, respectively, with the increase in the moisture content from 7.34 to 21.58% dry basis. This showed that seeds are more flexible in the horizontal orientation. |
Soluble and insoluble pollutants in fog and rime water samplesJaroslav FI©ÁK, Valeria STOYANOVA, Pavel CHALOUPECKÝ, Daniela ŘEZÁČOVÁ, Tsenka TSACHEVA, Temenujka KUPENOVA, Miko MARINOVSoil & Water Res., 2009, 4(10):S123-S130 | DOI: 10.17221/473-SWR Fog and rime water samples were collected at the meteorological observatory Mileąovka in February and June 2006. In the samples, the soluble and insoluble pollutant concentrations were evaluated separately and the differences between the fog and rime water samples were studied. The comparison of the fog and rime water samples indicates that the mean soluble component concentrations in the air appear to be higher during the rime events than during the fog events at Mileąovka. We recorded a larger mean particle size of the insoluble compounds in the fog water samples than in those of rime water. Some elements contained in the insoluble particles like Ca, Cl, C, Cu, Ag, were present largely in fog whole others, like Fe, Al, Si, Ti, prevailed in rime. In addition to the overall evaluation, the backward air trajectories were determined for each fog/rime event and the concentrations are presented as depending on the direction of the air particle transfer. |
How does legacy of agriculture play role in formation of afforested soil properties?D. Kacálek, J. Novák, D. Duąek, J. Bartoą, V. ČernohousJ. For. Sci., 2009, 55(1):9-14 | DOI: 10.17221/74/2008-JFS Soil properties of forest ecosystems depend on synergy of both parent material and organisms living in the soil, i.e. tree species communities including related plant and animal species. However these soils were not left intact being converted into agricultural land; addition of both nutrients and organic matter and cultivation using tillage led to increased fertility of topsoil. Even long-term afforested soils show differences which are considered as legacy of past agriculture. The change remains detectable for decades; though the altered properties are obvious especially couple of years after planting (approximately 10 years). We found increased concentrations of nutrients (P, K, Ca, and Mg) and subsequent increased base saturation (V %) in former tilled soil only. Moreover, there were no differences between topsoil and subsoil properties (69% and 72%, respectively). In addition to significantly lower saturation (both 0-10 cm and 11-30 cm layers) detected in the long-term-forest and 50-year-afforested (both covered with Norway spruce stands) soils in comparison with adjacent 10-year-old afforestations, there was found significantly lower base saturation in topsoil horizons compared to underlying ones. |
Perception of the forest by the villagers of the Salonga National Park in the Democratic Republic of CongoJ. M. IbesoaJ. For. Sci., 2009, 55(7):339-344 | DOI: 10.17221/92/2008-JFS Effective forest management demands detailed knowledge of resources. Local forest users are usually the best source of information. In the Democratic Republic of Congo forest management partnerships between local people and government staff have become more common. A proper understanding of local perceptions of the forest is needed. This study was designed and guided by a multidisciplinary team including a sociologist, a linguist and a forester. It identified and compared classification and management units. It also touched on the different uses of the forest products, the species that provided these products and the locations of these species. |
Selected food verticals and their impact on prices of food productsS. KríľováAgric. Econ. - Czech, 2009, 55(2):94-101 | DOI: 10.17221/581-AGRICECON The article deals with the analysis of food market on the level of consumer prices structure, through the identification of margins in the selected food verticals and through the analysis of consumer behaviour associated with changes in prices and income of population. The article also discusses the factors which may affect the current growing trend in prices of food in the SR and worldwide and subsequently, the reduction of gap between the Slovak and the EU prices. |
Seasonal Evolution of Hydrophilic and Hydrophobic Peptide Contents in Cheeses Made from Ewe's Goat's or Cow's MilkA. M. Vivar-Quintana, M.A. Blanco López, I. Revilla, I. González-Martín, J. M. Hernández-Hierro, C. González-PérezCzech J. Food Sci., 2009, 27(10):S106-S108 | DOI: 10.17221/950-CJFS Proteolysis is the principal and most complex biochemical event occurring during the maturation of the majority of ripened cheese varieties. In addition to softening the cheese body, proteolysis influences the development of cheese flavour via the formation of amino acid and peptides which make a direct contribution to flavour. Goat, cow and sheep cheeses have been elaborated with raw milk and calf rennet. The extent of proteolysis was monitored over six months of ripening and means of HPLC peptide profile analysis. The influence of season on the changes in hydrophobic and hydrophilic peptides and the HO/HI ratio during the ripening of the cheeses were studied. |
Phytoextraction of cadmium, copper, zinc and mercury by selected plantsL. Kacálková, P. Tlustoą, J. SzákováPlant Soil Environ., 2009, 55(7):295-304 | DOI: 10.17221/100/2009-PSE During the years 2006 and 2007 the phytoextraction ability of maize (Zea mays), willow-tree (Salix smithiana) and poplar (Populus nigra × P. maximowiczii) to accumulate cadmium, copper, mercury and zinc was investigated. Small scale field experiment was carried out on soil contaminated with chemicals from the waste incineration plant in Hradec Kralové (Czech Republic). Screening of this allotment showed very different contamination of all observed risk elements on places where the material intended to be burnt without safety of leakage into soil. Grown plants showed the different accumulation of observed elements in plant tissues as well as the influence of total content of the risk elements in soil. The highest Cd (1.5-1.73 mg/kg) and Zn (242-268 mg/kg) concentrations were found in willow-tree biomass mainly in the leaves. Cu and Hg were mostly accumulated by maize roots (14.6-15.8 mg Cu/kg and 1.3-7.4 mg Hg/kg) and lower amount was found out in willow-tree leaves again. In reference to total production of each plant the maximum Cd and Zn uptake by aboveground biomass was found in poplars (201 mg Cd/m2 and 38 200 mg Zn/m2) and maize, which showed high Zn uptake. The biggest amount of copper (2563 mg Cu/m2) was accumulated by aboveground maize biomass on the collection point with the highest Cu concentration in soil and by poplar (2394 mg Cu/m2) on the other collection point. The highest Hg uptake differs in reference to total Hg content in soil; willow-tree has the highest uptake on the place with lower Hg content in soil (44.6 mg Hg/m2) and maize has the highest uptake on the place with higher Hg content in soil (92 mg Hg/m2). |
Growth of wild cherry (Prunus avium L.) in a mixture with other species in a demonstration forestR. Stojecová, I. KupkaJ. For. Sci., 2009, 55(6):264-269 | DOI: 10.17221/71/2008-JFS Wild cherry is one of the noble hardwood species that increase the biodiversity of our forests and at the same time it could increase the income for forest owners. The preconditions for achieving these goals are the high quality of stem and appropriate silvicultural management. This means that wild cherry should occupy the main crown layer in the stand. The height/frequency diagram depicts two groups of wild cherry trees in the stand belonging to dominant/codominant and suppressed tree classes. Height periodic increment (measured between the years 2001 and 2007) is significantly (p < 0.01) different in these two groups confirming that there is no transition chance for the trees from the suppressed group to become a part of the main crown layer and play the role of future crop tree. The same is true of the diameter/frequency diagram which also has a two-peak shape remaining also at the end of the surveyed period. Our result suggests that silvicultural care should be focused only on trees belonging to future crop trees. |
Sustainability of dairy production in Turkey: A case studyH. Akcaoz, H. KizilayAgric. Econ. - Czech, 2009, 55(1):25-32 | DOI: 10.17221/2504-AGRICECON The aim of this paper is to show some empirical evidence of the sustainability and decision making in dairy production in Turkey. The main material of the study was obtained from the data collected by the survey conducted in the villages in Merkez, Manavgat and Serik districts of the Antalya province. Within the context of the research, 75 farmers were interviewed in the 2007 production season. The research results indicate that the households are the most important decision maker in terms of farming decisions. 56.3% of the farmers in the small farms, 61.5% of the farmers in the medium-size farms and 41.2% of the farmers in the large farm noted that they could quit dairy production activity if they received a fixed salary. |
Determination of antagonistic starter cultures for pickle and olive fermentation processesAhmet Hilmi Çon, Nihat KarasuCzech J. Food Sci., 2009, 27(3):185-193 | DOI: 10.17221/86/2008-CJFS In the present study, the main purpose was the selection of antagonistic starter cultures for pickle and olive fermentation processes. The chemical and microbiological properties of fermented 70 pickle and 16 olive samples collected from the province of the west part of Turkey were analysed. Subsequently, lactic acid bacteria strains producing bacteriocin-like metabolites were isolated and identified. From 86 samples, 16 isolates were chosen, depending on their partial antimicrobial activity against at least one selected indicator. 13 out of this 16 isolates were identified as Lactobacillus plantarum whereas 3 of them proved to be Lactobacillus pentosus. Moreover, all the relevant isolates were found to be potent acid producers. All these results obviously suggest that the isolated Lactobacillus plantarum 9 and 25 were appropriate for them to be proposed as starter cultures in fermented pickle and olive production. |
Tyramine Production by Enterococci from Various Foodstuffs: A Threat to the ConsumersK. Trivedi, I. Borkovcová, R. KarpíąkováCzech J. Food Sci., 2009, 27(10):S357-S360 | DOI: 10.17221/934-CJFS Tyramine is the most frequent biogenic amine found in cheese and is also frequently found in other fermented foods and beverages. In total 273 different strains of enterococci from various foodstuffs were studied. A multiplex PCR was designed for the genotypic differentiation of various enterococci strains at species level and for determination of the presence of tyramine producing (tyrdc) gene. E. faecalis and E. faecium were found to be prominent strains in dairy and meat products while, E. faecium and E. mundtii were found to be ruling in case of fruits and vegetables. HPLC analysis was carried out for determination of levels of tyramine. 211 i.e. 86.8% of samples were containing about 1000-1500 mg/l of tyramine, while 10 i.e. 4.1% were found to have tyramine in the range 100-500 mg/l. Negative samples showed 0 mg/l of tyramine. |
Assessing radish (Raphanus sativus L.) potential for phytoremediation of lead-polluted soils resulting from air pollutionSo. Asadi Kapourchal, Sa. Asadi Kapourchal, E. Pazira, M. HomaeePlant Soil Environ., 2009, 55(5):202-206 | DOI: 10.17221/8/2009-PSE The objective of this study was to investigate the capability of radish to extract lead from soils contaminated with lead resulting from air pollution. A randomized block experiment design was performed. The soil was contaminated with PbNO3 and the treatments consisted of 180 (standard), 250, 350, 450, 800 and 1000 mg/kg lead. After development, plants were harvested and divided into shoots and roots. The lead content of each plant part as well as the soil-lead were measured. The results indicated a non-linear positive relation between the lead concentrations in soil and that accumulated in plant roots and shoots. By increasing the lead concentration in soil, its accumulation in plant tissues was also increased. Most of the extracted lead was accumulated in the roots (208.1 mg/kg) compared to shoots (27.25 mg/kg). Since radish can be seeded up to five times a year, and its yield may reach up to 20 t/ha, it can be used to remediate lead-polluted topsoils (0-10 cm). |
Effect of zinc foliar application on grain yield of maize and its yielding componeJ. Potarzycki, W. GrzebiszPlant Soil Environ., 2009, 55(12):519-527 | DOI: 10.17221/95/2009-PSE Actual yields of maize harvested by farmers are at level much below attainable yield potential of currently cultivated varieties. Among many growth factors zinc was recognized as one of main limiting factors of maize crop growth and yielding. This hypothesis has been verified within a three-year field study, where zinc fertilizer was applied to maize plants at the 5th leaf stage. Maize crop responded significantly to zinc foliar application in two of three years of study. The optimal rate of zinc foliar spray for achieving significant grain yield response was in the range from 1.0 to 1.5 kg Zn/ha. Grain yield increase was circa 18% (mean of three years) as compared to the treatment fertilized only with NPK. Plants fertilized with 1.0 kg Zn/ha significantly increased both total N uptake and grain yield. Yield forming effect of zinc fertilizer revealed via improvement of yield structure elements. The number of kernels per plant showed the highest response (+17.8% as compared to the NPK plot) and simultaneously the highest dependence on N uptake (R2 = 0.79). For this particular zinc treatment, however, the length of cob can also be applied as a component of yield structure significantly shaping the final grain yield. |
The effect of genotype, housing system and egg collection time on egg quality in egg type hensE. Tůmová, M. Skřivan, M. Englmaierová, L. ZitaCzech J. Anim. Sci., 2009, 54(1):17-23 | DOI: 10.17221/1736-CJAS The effect of egg collection time, genotype and housing system on egg quality characteristics was evaluated in an experiment with laying hens of ISA Brown, Hisex Brown and Moravia housed on litter and in conventional cages. The experiment was conducted from 20 to 64 weeks of age. Eggs were collected and recorded daily at 06:00, 10:00 and 14:00 h. Once every four weeks, two days in row, all eggs laid from each pen or cage at each oviposition time were used for egg quality analyses (total 1 694 eggs). Egg collection time was dependent on genotype. Significantly more eggs were laid at 06:00h by ISA Brown while Moravia laid eggs mainly at 10:00 and 14:00 h. Housing on litter postponed the time of oviposition. The highest egg weight (62.09 g) was recorded in Hisex Brown genotype placed in cages and at 06.00 h. Interactions of all factors were determined also in Haugh units (P ≤ 0.041). Eggshell strength was affected by housing system, genotype and egg collection time (P ≤ 0.049). It was higher in cages than on litter, and lower in the Moravia genotype in comparison with the other two strains. |
Study of genetic differences among Slovak Tsigai populations using microsatellite markersS. Kusza, E. Gyarmathy, J. Dubravska, I. Nagy, A. Jávor, S. KukovicsCzech J. Anim. Sci., 2009, 54(10):468-474 | DOI: 10.17221/1670-CJAS In this study genetic diversity, population structure and genetic relationships of Tsigai populations in Slovakia were investigated using microsatellite markers. Altogether 195 animals from 12 populations were genotyped for 16 microsatellites. 212 alleles were detected on the loci. The number of identified alleles per locus ranged from 11 to 35. In the majority of the populations heterozygosity deficiency and potential risks of inbreeding could be determined. High values of FST (0.133) across all the loci revealed a substantial degree of population differentiation. The estimation of genetic distance value showed that the Slovak Vojin population was the most different from the other populations. The 12 examined populations were able to group into 4 clusters. With this result our aim is to help the Slovak sheep breeders to establish their own mating system, to avoid genetic loss and to prevent diversity of Tsigai breed in Slovakia. |
Preliminary results of in vivo thermotherapy of plum, apricot and peach cultivars artificially infected with PPV-M and PPV-D strains of Plum pox virusJ. Polák, A. HauptmanováHort. Sci. (Prague), 2009, 36(3):92-96 | DOI: 10.17221/47/2008-HORTSCI The elimination of Plum pox virus (PPV) in different stone fruit cultivars was verified by the method of thermotherapy in vivo. Trees of two plum cultivars Čačanská lepotica and ©vestka domácí, apricot cultivars Leskora and Velkopavlovická, and peach cultivars Redhaven and Earliglo were used. They were infected artificially with two strains of the virus (PPV-D, PPV-M). Two cycles of thermotherapy in vivo were performed. During the first cycle, 16 trees of plum, apricot and peach were treated for 15 days at 37°C. In the second thermotherapy cycle, 10 trees of individual cultivars of plum, apricot and peach were treated for 22 days at 37°C. In the first thermotherapy (T1), 8 trees out of 16 died; PPV was eliminated in 2 trees of cv. Čačanská lepotica, 1 tree of cv. ©vestka domácí and 2 trees of cv. Velkopavlovická. In the second thermotherapy (T2), 1 of 10 treated trees died. The virus was eliminated in 2 trees of cv. Čačanská lepotica, 1 tree of cv. Leskora, 2 trees of cv. Velkopavlovická, and 1 tree of cv. Redhaven. Nine (T1) and seven (T2) months after the thermotherapy, the presence of PPV was detected in 6 out of 11 originally recovered trees using ELISA. Out of 26 trees, 4 trees remained recovered: 2 plum trees and 2 apricot trees. One of these trees, apricot cv. Leskora was originally infected with PPV-M strain, whereas the other three with PPV-D strain. None of the 10 peach trees was treated successfully. |
The sensory characteristics of berry-flavoured kefirLütfiye Yilmaz, Tülay Özcan Yilsay, Arzu Akpinar BayizitCzech J. Food Sci., 2006, 24(1):26-32 | DOI: 10.17221/3290-CJFS Certain physicochemical and sensory characteristics of the flavoured kefir drink were studied during refrigerated storage. Kefir drink batches were prepared using 2% addition of kefir grains, incubation for 18 h and subsequent filtration. The batches were then flavoured with blackberry, raspberry and strawberry aromas in the concentrations of 0.05, 0.10 and 0.15%, respectively. The samples were taken for analysis on 1st, 4th, 7th, and 10th days of storage at 4 ± 1°C. The sensory analysis of the flavoured kefir samples revealed the best acceptability level on the 4th day of storage. Nevertheless, the samples were acceptable all throughout the storage. The percentage of the aroma addition significantly influenced the odour, flavour, texture, and mouth-feel, and the overall acceptability ratings. The higher percentage of the added aroma the more sensed, however, the lowest aroma addition was preferable. The pHs of the flavoured kefir drinks decreased throughout the storage time whereas, the titratable acidity, alcohol content and CO2 values increased. |
Different estrous induction methods during the non-breeding season in Kivircik ewesI. Dogan, Z. NurVet Med - Czech, 2006, 51(4):133-138 | DOI: 10.17221/5532-VETMED The efficiency of medroxyprogesterone acetate (MAP) sponges in combination with either pregnant mare serum gonadotrophin (PMSG) or cloprostenol (PGF2α) for inducing and synchronizing the estrous cycle in non-lactating Kivircik ewes was investigated during the natural non-breeding season. All ewes (n = 69) were treated with 60 mg MAP sponges for 12 days. In addition, each ewe received an intramuscular injection of either 1.5 ml sterile saline solution (n = 18); 125 μg PGF2α (n = 14); 500 IU PMSG (n = 18) or 500 IU PMSG and 125 μg PGF2α (n = 19), 48 h before the sponge removal. Cervical artificial insemination (AI) with diluted fresh semen was performed at a fixed time (48 and 60 h) following progestagen withdrawal. The different groups estrous response for the first 24 ± 6 h and within 120 h, time to onset and duration of the induced estrous, and pregnancy rate was found to be 36.2%, 81.6%, 41.7 ± 2.3 h, 29.6 ± 1.5 h, and 54.5%, respectively. There were significant differences between groups MAP and MAP/PGF2α and their with the two latter groups (MAP/PMSG, MAP/PMSG/PGF2α) in terms of the onset of induced estrous (P < 0.05) and between groups MAP and MAP/PGF2α in terms of the duration of induced estrous (P < 0.05) and between the first two groups (MAP, MAP/PGF2α) and the latter two groups (MAP/PMSG, MAP/PMSG/PGF2α) in terms of estrous response at the first 24 ± 6 h (P < 0.05). These results indicate that, the use of MAP/PMSG, rather than MAP or MAP/PGF2α, was effective in the attainment of early and compact induction and synchronization of estrous in non-lactating Kivircik ewes during the natural non-breeding season. |
Two rapid diagnostic procedures for the identification of Campylobacter jejuni/coli in food matrixTomáą Jeleník, Zdeňka ©abatková, Kateřina Demnerová, Jarmila PazlarováCzech J. Food Sci., 2005, 23(3):121-125 | DOI: 10.17221/3381-CJFS Campylobacter species, in particular C. jejuni and C. coli, cause infections which vary in symptoms, ranging from asymptomatic to severe chronic illness. The only ISO method for the detection of Campylobacter spp. until now has been the cultivation by selective enrichment and distinct conditions of growth taking several days to complete. We compared the Singlepath® Campylobacter test which involved 24 h of enrichment in Bolton broth, with PCR-based identification. Chicken meat salad with mayonnaise was spiked with C. jejuni and C. coli and the detection limit was determined. PCR provided the same detection limit of 102 CFU/ml for both strains. The immunotest Singlepath® was positive with C. jejuni only, the quantity of cells being 103 CFU/ml. C. coli was undetectable by Singlepath®, even the concentration of 105 did not reveal a positive reaction. |
PCR-based detection of cow's milk in goat and sheep cheeses marketed in the Czech RepublicEva Maąková, Ivana PaulíčkováCzech J. Food Sci., 2006, 24(3):127-132 | DOI: 10.17221/3307-CJFS A method based on the polymerase chain reaction (PCR) principle was validated for detecting cow's milk in goat and sheep cheeses. DNA was isolated from the cheeses using the isolation kit Invisorb Spin Food I by Invitek Co., designed for the samples of animal origin. The PCR method applied utilizes the sequence of the mitochondrial gene coding cytochrome b which is specific for mammals. It uses the common forward primer and the reverse primer species-specific. After electrophoresis, cow DNA was characterised by the fragment of the size of 274 bp, goat DNA by the fragment of 157 bp, and sheep DNA by the fragment of 331 bp. The detection limit of the PCR method described (1%) was determined with model samples made from pure goat cheese with a defined addition of cheese made from cow's milk. The method validated was applied in the analysis of 17 goat cheeses and 7 sheep cheeses obtained from retail trade. Products of Czech, Slovak, French, Dutch, and Italian origin were examined. The presence of undeclared cow's milk was detected in three kinds of goat cheese and in one of sheep cheese. |
Development of testicular dimensions and size, and their relationship to age and body weight in growing Kivircik (Western Thrace) ram lambsM. Koyuncu, S. Kara Uzun, S. Ozis, S. DuruCzech J. Anim. Sci., 2005, 50(6):243-248 | DOI: 10.17221/4164-CJAS Forty-seven Kivircik ram lambs, aged 2 months, raised and managed under an intensive system were used to measure the development of testis diameter and length, and scrotum circumference, length and volume, and their association with the development of body growth. The effects of age and body weight of lambs, type of birth and dam age on the development of the five testicular parameters from weaning at 2-6 months of age were investigated. In ram lambs, the respective least-squares means of testis measurements: testis diameter, testis length, scrotum circumference, scrotum length and scrotum volume were 2.55 ± 0.099 cm, 5.83 ± 0.165 cm, 15.23 ± 0.650 cm, 7.24 ± 0.287 cm and 87.57 ± 5.921 cm3 at 2 months; and 2.99 ± 0.099 cm, 6.59 ± 0.164 cm, 14.24 ± 0.657 cm, 10.60 ± 0.290 cm and 157.49 ± 5.987 cm3 at 6 months. The effect of birth type on testis diameter and the effect of dam age on testis length were significant (P < 0.05 and P < 0.01), besides the effects of lamb age and live weight of lambs on the whole testis measurements were significant (P < 0.01). |
