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The analysis of the trace elements has been shown to be a valuable tool to discriminate wines according to their region of origin. As, Ba, Ca, Co, Cr, Li, Mg, Rb, Sn, Sr, and V were selected as specific markers indicating the origin of Slovak wines according to the vineyard regions. Several factors, such as the environmental contamination, agricultural practices, climatic changes, and others, may markedly change the multielement composition of the wine and may endanger the relationship between the wine and the soil composition. The effect was studied of the viniculture process on the distribution of selected markers in the winemaking products. The main markers pass from the vineyard soil to the grape, and the main portion leaves the winemaking process in the press cake and yeast lees. Very significant correlation of the wine origin markers was found between changed the wine making products and the vineyard soils. The sugar addition to grape juice to some extent the total element compositions of wines but did not result in substantial changes of the markers determining the wine origin.

For characterization of the ability of crops to reflect changing soil properties after the addition of ameliorative materials into the soil both pot and rhizobox experiments were provided. In the pot experiment, the influence of the addition of lime and limestone into contaminated Cambisol containing 7.14 mg Cd/kg, 2174 mg Pb/kg, and 270 mg Zn/kg on element availability for spring wheat was tested. The ameliorative materials were added into the pots containing 5 kg of soil in amount of 3 g CaO, and 5.36 g CaCO₃ per kg of the soil. Soil pH reached up to 7.3 in lime treatments compared to 5.7 in control soil. Mobile portion of soil elements (0.01 mol/l CaCl₂ extractable) dropped by 80% for Zn, 50% for Cd, and 20% for Pb, respectively. In both straw and grains of wheat reduced content of elements was observed in limed pots compared to the control ones. For a detailed characterization of the influence of root exudates on the strength of developed complexes in comparison with the bulk soil, short-term rhizobox experiment was set up under identical soil and lime treatments. Generally, the results of rhizobox experiment confirmed the findings from the pot experiment discussed above. Content of elements in shoots and roots of wheat dropped mainly in the case of Cd and Pb. Soil mobile portion of all three tested elements introduced clear depletion curve in control treatment, both limed treatments showed high stability of element complexes almost unaffected by wheat roots.

High mortality of nestling cockatiels (Nymphicus hollandicus) was observed in one breeding flock in Slovakia. The nestling mortality affected 50% of all breeding pairs. In general, all the nestlings in affected nests died. Death occurred suddenly in 4- to 6-day-old birds, most of which had full crops. No feather disorders were diagnosed in this flock. Two dead nestlings were tested by nested PCR for the presence of avian polyomavirus (APV) and Chlamydophila psittaci and by single-round PCR for the presence of beak and feather disease virus (BFDV). After the breeding season ended, a breeding pair of cockatiels together with their young one and a fledgling budgerigar (Melopsittacus undulatus) were examined. No clinical alterations were observed in these birds. Haemorrhages in the proventriculus and irregular foci of yellow liver discoloration were found during necropsy in the young cockatiel and the fledgling budgerigar. Microscopy revealed liver necroses and acute haemolysis in the young cockatiel and confluent liver necroses and heart and kidney haemorrhages in the budgerigar. Two dead cockatiel nestlings, the young cockatiel and the fledgling budgerigar were tested positive for APV, while the cockatiel adults were negative. The presence of BFDV or Chlamydophila psittaci DNA was detected in none of the birds. The specificity of PCR was confirmed by the sequencing of PCR products amplified from the samples from the young cockatiel and the fledgling budgerigar. The sequences showed 99.6-100% homology with the previously reported sequences. To our knowledge, this is the first report of APV infection which caused a fatal disease in parent-raised cockatiel nestlings and merely subclinical infection in budgerigar nestlings.

PATRIA Kobylí, Inc. is one of the traditional wine-growers and producers of quality wines based in southern Moravia. Farming on 152 hectares, PATRIA Kobylí supplies the market with more than 1 million litres of wine per year. It offers an assortment of 14 whites and 6 blue wines. The company focuses on wines designed for gastronomes and distributed to selected specialized wine-merchants. But since 2003, the sales of the company's wines have been declining. The sales in the individual months of 2006 were the lowest for the last 6 years. But the aim of the company will not be to increase the sales volume but the wine quality and services and sales techniques and so to maintain its position in the market. The company started to analyze its strengths and weaknesses and extended its portfolio of Saint Martin wine and special wines and sparkling wines.

Fifteen coffee creamers, 10 cream aerosols, and 5 bouillon cubes from the retail market were analysed, principally for their contents of trans-fatty acids that are known to increase the risk of coronary heart disease, and for their contents of 3-chloropropane-1,2-diol (3-MCPD) fatty acid esters that possibly have a bioaccumulation potential. The contents of trans-fatty acids in coffee creamers, cream aerosols and bouillon cubes were in the range of 0.2-32.8%, < LOD - 6.0%, and 0.5-2.1% of total fatty acids, respectively. All samples contained high levels of 3-MCPD fatty acid esters that were determined after releasing the free 3-MCPD by methanolysis. The 3-MCPD levels in coffee creamers, cream aerosols, and bouillon cubes were in the range of 130-730 µg/kg (540-4480 µg/kg fat), 50-730 µg/kg (220-2880 µg/kg fat), and 380-670 µg/kg (2650-4840 µg/kg fat), respectively. The results showed that the refined and hydrogenated oils may represent a certain risk. The highest levels of 3-MCPD esters were found in a sample of refined palm oil (4170 µg/kg). Currently, there is no information available on how these 3-MCPD esters are metabolised, to which extent they are hydrolysed or biosynthesised in the body, to which extent they are deposited in tissues, and how they influence the properties and functions of tissues (if they really do it).

The toxicity of three concentrations (5%, 10% and 20% w/v) and spraying schedules (2, 4 and 6 weekly applications) of an extract from West African black pepper, Piper guineense, for managing two major post-flowering pests of cowpea, Vigna unguiculata, was investigated in two cropping seasons at the Research Farm of the Institute for Agricultural Research, Ahmadu Bello University, Zaria, Nigeria. The insect pests were the larvae of Maruca vitrata (Lepidoptera: Pyralidae) and the cowpea coreid bug, Clavigralla tomentosicollis (Hemiptera: Coreidae). The higher concentrations (10% and 20% w/v) and more frequent applications (4 and 6/week) significantly (P < 0.05) reduced the numbers of the two insect pests compared to the untreated control in both years. Pod damage was significantly reduced and grain yields consequently increased in treated plots compared with the other extract treatments and the untreated control. West African black pepper extract applied at higher concentrations and more frequently could play an important role in integrated management of pests' infestations on field cowpea managed by limited resource farmers in third world countries.

Wild potato species (genus Solanum, section Petota) represent a tremendously diverse gene pool which is traditionally utilized as a source of diverse traits for potato breeding. Abiotic and biotic stress tolerance and resistance belong to the most frequently utilized traits of wild species in potato breeding programs. This review provides an introduction to the taxonomy, centre of diversity, genetic characteristics, evolution and important tolerance and resistance traits of wild potatoes and their use for potato breeding. The review has been written for readers who are interested in the problems of finding and utilization of new resistance genes from the wild genetic resources.

Significant quantities of Cd have been added to soils globally due to various anthropogenic activities, posing a serious threat to safe food production and human health. Rhizosphere, as an important interface of soil and plant, plays a significant role in the agro-environmental system. This article presents a review of relationship between root excretion and microorganisms and plant resistance to Cd toxicity and possible mechanisms. Root exudates markedly altered in species and quantity under Cd stress. Root exudates can affect Cd absorption by plants through changing the physical and chemical characteristics of rhizospheres. The influence of root exudates on Cd bioavailability and toxicity may include modifying the rhizosphere pH and Eh, chelating/complexing and depositing with Cd ions, and altering the community construction, the numbers and activities of rhizospheric microbes. In this paper, the methods to reduce the transfer of Cd in soil-plant system by adjusting rhizosphere environment are discussed, and some aspects are also proposed that should be emphasized in the future research work.

he study was undertaken to determine a possible role of polyamines (putrescine, spermidine, spermine) as antioxidants in salt tolerance of Chamomilla recutita and Origanum majorana. Salinity generally induced variable changes in growth, contents of oil and flavonoids of both plants; foliar application of any polyamines counterbalanced the effects of salinity. In general, the degree of stimulation differed according to the type and concentration of the used additive and the type of the plant.

The aim of the study was to compare physiological statuses of stripped and not stripped tench females with ovulation induced by GnRHa or carp pituitary extract (CPE). The comparison was based on selected biochemical stress indices (cortisol, glucose), condition (TP, TGA), and cell membrane characteristics (ALT, AST, CK). In the first and in the second year, 37 and 29 tench females were monitored, respectively. No differences in the values of biochemical parameters in either period were found between the two groups of stripped female tench whose ovulation was induced by GnRHa and CPE, respectively. No significant differences in TP, TGA, ALT, AST and CK values were found between the groups of stripped and not stripped females with ovulation induced by GnRHa or CPE. Different values were found in stress indices, specifically in glucose concentrations. A significant increase (P < 0.01) in glucose concentrations was found in tench females immediately after stripping and, in not stripped females, about 48 hrs after ovulation induction. Both artificial stripping and the inability to release eggs are important stress factors for female tench.

In the territory of the Czech Republic there are more than 50% of agricultural soils exposed to water erosion; it is a very urgent problem both at present and for the future. It must be solved now when there is still something to be protected. It is rather complicated to describe the soil properties in terms of soil susceptibility to water erosion because it is a complex relation in which many factors participate. For the complex evaluation of all main factors participating in erosion origination it is possible to apply the Universal Soil Loss Equation (USLE). It consists of six factors interacting with each other and participating in the origination of soil erosion. One of these factors is the soil erodibility factor (K-factor), the revision of which for soil conditions of the CR is the subject of this study. In total ca. 5000 soil pits from the whole territory of the country were processed and evaluated in detail. The main results of this study are K-factor values (means and variances) for the soil types, subtypes and varieties (represented in the database) according to the Taxonomic Classification System of Soils of the Czech Republic.

The available data concerning rapid identification of fish bacteria via commercial phenotypic tests demonstrate that there is no agreement regarding the choice of the tests. However, API 20E, an identification system for Enterobacteriaceae and other non-fastidious Gram-negative rods developed for clinical specimens, seems to be increasingly used for the identification of fish pathogens. In this review, adaptation of API 20E for fish bacterial isolates and its distinctiveness for fish bacteria was assessed. Some strains are wrongly identified because they are not included in the database of API 20E system. API 20E reactions should be compared with the diagnostic schemes based on reactions in conventional phenotypic tests. Due to their significance for fish health and impact on the aquaculture, and because of the need for their rapid identification, some important fish bacteria should be included in the API 20E system, such as Yersinia ruckeri, Edwardsiella ictaluri, Vibrio anguillarum.

Damage caused by bark peeling and browsing by red deer to Picea abies was investigated in two localities in the Českomoravská vrchovina Highlands. 127 experimental plots of 50 × 50 m in size were monitored in stands aged from 15 to 93 years. In total, 44% of 16,700 inspected trees were damaged by bark peeling and browsing. The area of gaping wounds varied from 50 to 1,650 cm². Stem decay affected 68% of the damaged trees. Some properties of the decay were analysed from 200 sample trees felled on the plots. Bark peeling damage occurred when the trees were 4 to 48 years old. Decayed wood accounted for 22-70% (mean 42%) of the merchantable stem volume of sample trees. The mean rate of the vertical decay spread was 17.4 cm per year (ranging from 4-63 cm per year). Mean decay volume correlated positively and mean spreading rate correlated negatively with the time elapsed from the bark peeling damage occurrence.

The concentrations of cadmium, lead and zinc were determined in the soft tissue of oysters (Ostrea edulis L.) from three locations in the Mali Ston Bay on the south-eastern coast of the Adriatic Sea. The sampling was performed during two periods of breeding cycle, in summer (age ~1 year) and winter (age ~20 months, consumption size). The concentrations of cadmium, lead and zinc measured in the oyster soft tissue showed considerable spatiotemporal variations that could be attributed to seasonal differences in the freshwater inflow which varied between the study locations. Median concentrations measured at different locations and in different time periods ranged from 0.303 to 0.969, 0.13 to 0.32 and 208.9 to 650.0 µg/g wet weight for cadmium, lead and zinc, respectively. Although metal concentrations do not exceed the maximal legally approved limits according to the legislation of the Republic of Croatia and EU, more knowledge of their spatiotemporal distribution - of cadmium in particular - would contribute to the sustainable future development of oyster farming in Croatian waters.

The research analyses the fundamental resources, farm structures, and composition by aim of farming, labour force and family income sources of the agricultural private holdings from the aspects of subsistence and income generation as well as of rural development. By processing the various databases, the research provides a clear picture on the situation of private holdings and groups of farms (self-supplying, marketing the surplus, commercial holdings). On the basis of the analyses, it is possible to estimate the number of competitive farms, that is, of professional farms, and the number of the producers who are not engaged in commercial production. This points out the need to extend the sphere of rural activities are connected to the other sectors of the national economy.

Ditylenchus dipsaci, the stem nematode, is a migratory endoparasite of over 500 species of angiosperms. The main method of D. dipsaci control is crop rotation, but the presence of morphologically indistinguishable host races with different host preferences makes rotation generally ineffective. Therefore, a sensitive, rapid, reliable, as well as cost effective technique is needed for identification of D. dipsaci in biological samples. This study describes the development of species-specific pairs of PCR oligonucleotides for detection and identification of the D. dipsaci stem nematode in various plant hosts. Designed DIT-2 primer pair specifically amplified a fragment of 325 bp, while DIT-5 primer pair always produced a fragment of 245 bp in all D. dipsaci isolates. Two developed SCAR primer pairs were further tested using template DNA extracted from a collection of twelve healthy plant hosts; no amplification was however observed. The developed PCR protocol has proved to be quite sensitive and able to specifically detect D. dipsaci in artificially infested plant tissues.

To determine the effect of weaning system on Awassi sheep milk production and lamb growth, 68 Awassi ewes and their lambs (n = 104) were assigned to one of the following treatment groups a) after 3 days from birth (MIX), ewes were separated from their lambs during the evening for 15 h and milked once daily in the morning, and their lambs were allowed to suckle for 9 h during the day or b) ewes were not milked and suckled by their lambs (DY60) during the first 60 days of lactation. Ewes and their lambs were assigned to the groups according to ewe's age, parturition date, sex of the lamb and birth type. All lambs were weaned at 60 days of age and the ewes were milked twice daily. Milk yield was recorded fortnightly and milk fat composition was recorded monthly. The birth weight of lambs was recorded in the first 2 hours and they were weighed fortnightly until 240 days of age. Average lactation lengths (including suckling and milking) in MIX and DY60 group were similar (171.21 ± 5.40 and 168.36 ± 4.87 days, respectively). Differences between MIX and DY60 group in commercial milk yield (103.25 ± 7.93 and 63.59 ± 5.41 kg, respectively) and milk fat yield (5.88 ± 0.64 and 3.62 ± 0.31 kg, respectively) were highly significant (P < 0.001). Growth performance of lambs was similar in both groups (P > 0.05), however, male lambs were heavier and grew faster than female lambs until weaning (P < 0.05) and 90 days of age (P < 0.001). The effect of birth type at 210 days of age for the female lambs in MIX group was found to be statistically significant (P < 0.05) and the mean weight of single born female lambs was 40.39 ± 1.27 kg, and 41.09 ± 1.22 kg for twin born female lambs. The effects of ewe's age for female lambs in MIX and DY60 group and birth type for the lambs in DY60 group were insignificant (P > 0.05). These results show that Awassi lambs can be successfully reared in MIX suckling system and ewes produce more commercial milk than in the traditional rearing system (DY60), which offers a potential increase in economic return for Awassi sheep producers.

The object of an experiment was inorganic and organic forms of zinc, copper and manganese applied in mineral mixtures to dairy cows. The experiment was carried out on 90 cows with average milk yield for previous lactation of about 9 500 kg milk. The cows received mineral mixtures containing inorganic or organic forms of zinc, copper and manganese for 6 weeks before calving and during the first three months of lactation. The application of microelements as bioplexes in amounts covering 30% of daily requirements of cows had a positive effect on an increase in colostrum dry matter content from 20.9 to 23.35% as well as on the concentration of protein and fat, and the level of minerals - calcium, phosphorus, magnesium, zinc and copper. In cows' milk in the 2^nd and 3^rd month of lactation there were no differences in contents of minerals. However in the blood serum of cows in the 1^st and 2^nd month of lactation an increase in calcium concentration from 1.96 to 2.14 g/kg was observed while the content of phosphorus also increased average from 1.76 to 2.22 g/kg in the first trimester of lactation.

Slow-Release Fertilizers (SRF) Silvamix Forte and Silvagen were tested in two-year experiments with container-grown woody plants (Pyracantha coccinea, Thuja occidentalis). Several fertilizing systems were compared: preplant application of SRF into substrate as the sole nutrient source for a two-year period, preplant application of SRF and soluble fertilizer (PG Mix), and preplant application of SRF and soluble fertilizer together with additional fertilizing by solution of nitrogen fertilizer during both growing periods. A system with controlled-release fertilizer (CRF) Osmocote 5-6 was chosen as a control variant; it was incorporated into substrate before planting in the first year and top-dressed in the second year. CRF Plantacote 6M (mixed into substrate before planting and top-dressed in the second year) and Osmocote 16-18 applied only before planting were tested, too. The experiments showed that SRF Silvamix Forte and Silvagen give results comparable with CFR provided that they were incorporated together with soluble ferti-lizer dose and plants were fertilized by solution of nitrogen fertilizer during both growing periods.

At present, photogrammetric interpretation of aerial images is a dominant method of forestry mapping. In the last years, transition from analogue to digital photogrammetry has been distinct. Digital photogrammetry enables to achieve workflow effectivity, and so to decrease the final product costs. The objective of the submitted paper was to evaluate the availability of digital photogrammetry for the forestry mapping rationalization. Digital aerotriangulation using the ImageStation SSK system brings more accurate results without requirements for the use of a larger amount of control points. The results also demonstrated the use of colour infrared aerial images, and also black and white aerial images at the scale 1:15,000 for the orthoimage creation in the forestry mapping department. Compared with the black and white aerial images, the colour infrared images have an essentially more interesting content, mainly from the qualitative aspect, which shifts them to use in many forestry disciplines (mostly for determination of the health conditions of forests stands, …), in combination with the remote sensing of the Earth and GIS (Geographic Information Systems).

This report describes a clinical case and development of the polyglandular failure syndrome including hypothyroidism and hypoadrenocorticism in a 6-year-old female Black Russian Terrier. The bitch was presented because of hypothyroidism on the first occasion. Oral supplementation with sodium levothyroxine resulted in clinical improvement. Four months later, it was presented again with similar and rather vague clinical signs and diagnosed with adrenocortical deficiency both in mineralocorticoid and glucocorticoid functions. Indirect immunofluorescence to investigate the presence of circulating autoantibodies against the thyroid and adrenal glands revealed only anti-thyroid antibodies (microsomal pattern) at a dilution of 1:40. Since then, the bitch has been on hydrocortisone and fludrocortisone with no adjustments necessary for a year. The combination of two endocrinopathies and the episodic course of hypoadrenocorticism made the final diagnosis difficult, posed a diagnostic challenge and required the owner's compliance with diagnostic and therapeutic procedures.

Development assistance achieved remarkable success in different periods. For example, Botswana and South Korea reached the great development in the 60s after very bad situation, Indonesia in the 70s, Bolivia and Ghana at the end of the 80s, Uganda and Vietnam in the 90s. In these countries development assistance played important role in economic transformation in formulation of the development of politics. The development assistance contributed educational programs and financially supported the development of public sector. The "Green Revolution" - by means of innovations in agriculture, investments and political changes - improved the live conditions of millions people thanks to the collaboration of many bilateral and multilateral donors. But there are some failures with the foreign aide. While the formed dictator of Zaire Mobutu Sese Seko became one of the richest people in the world (and invested his property in abroad), the development assistance did not stop for many years, Zaire (now the Democratic Republic of Congo) is only one example of the situation, where the permanent flows of assistance ignore or support the corruption and in suitable politics of governments. Tanzania received two milliards dollars for building the roads destiny the twenty years. But the roads were destroyed sooner, than the works could be finished because of insufficient maintenance. The study of World Bank brings the conclusions of the new conception of the development assistance: financial assistance works only in suitable political world; the lowering of poverty is possible only with working institutions - political and economic; effective assistance complete the private investments; receiving country is obliged to have public sector in function; the function of public sector is developing on the activity of civil society; patience and good ideas, not only money, can help to reforms in very unfavorable conditions.

The real trade development is estimated by the recalculation of nominal value through unit values (prices). The unit value indices reflect, besides the change of prices, also the changes in the structure and qualitative changes of the traded products. In the 70s, the average yearly increase of unit values reached 14% and reflected namely the inflation caused by the oil shocks. Since then, it reaches in average up to 1%. We can suppose that, under the normal conditions, unit values growth is rather the consequence of the qualitative and structural changes (increased share of the more sophisticated products of processing industry), in fact, prices in international trade have been decreasing for years already. The question whether the world trade growth rates decrease or accelerate is then still open. It is certain that the world trade volume growth rate (6.3%) has shown a considerable surpass to the production growth rate (4.1%).

The contribution presents the findings of the third phase of an extensive survey, the main goal of which was the identification of factors stimulating or restricting the establishment of small and medium-sized enterprises. It comprises the outcomes of the comparative analysis aimed at the comparison of groups of small and medium-sized enterprises and the model enterprise according to areas influencing founders' entrepreneurial activities.

The use of plants to remove heavy metals from soil (phytoremediation) is expanding due to its cost-effectiveness as compared to conventional methods and it has revealed a great potential. Since contaminants such as Pb or Cd have a limited bioavailability in the soil, methods to facilitate their transport to the shoots and roots of plants are required for successful phytoremediation. The objective of this study was to investigate the effects of addition of different rates (0, 3, 6 and 12 mmol/kg) of ethylene diaminetetraacetate (EDTA) on heavy metal availability in soils contaminated with 50 mg/kg Cd (CdCl₂), 50 mg/kg Cu (CuSO₄), 50 mg/kg Pb [Pb(NO₃)₂] and 50 mg/kg Zn (ZnSO₄), and on the capacity of canola (Brassica napus L.) and Indian mustard (Brassica junceaL.) plants to uptake Cu, Cd, Pb and Zn in a growth chamber. Results indicated that EDTA application increased heavy metal availability and uptake by plants. Significant differences were obtained in both species and plant parts. As for plant species tested, canola was more effective in the uptake of Cu, Cd, Pb and Zn. Root heavy metal uptake of both species was higher than shoot heavy metal uptake.

The basic concept of malt barley production is to reach a dilution effect of nitrogen accumulated by grains at maturity. A three-year study was undertaken to establish time courses of nitrogen (N) and sulphur (S) in leaves as the prerequisite tool for total grain yield prognosis. Sulphur application at the lowest rate of 25 kg/ha significantly increased yields of grain in 2001 and 2003. The time course of N and S concentrations in leaves over the growing season showed declining trends for N and variable for S. At mid tillering (BBA-25) both N and S, but at BBA-31 only N concentrations significantly responded to S rates, and in turn affected patterns of N concentration in barley organs up to maturity. Sulphur concentrations and N:S ratios were useful tools to make reliable prognosis of total grain yield of barley at BBA-31 as indicated by the obtained regression equations. The threshold values for N and S in leaves in order to achieve the maximum total grain yield are 0.4% for S and 8.0 for the N:S ratio as presented by the graphical procedure.

The exclusive pre-selective effect of BLAD carriers reproducing in the exposed Holstein-Friesian cattle population prompted to search for the candidate gene variants of high quality of milk performance traits within the bovine ITBG2 gene or loci linked with bovine ITBG2 gene. Theoretical considerations indicated that the &Idquo;silent mutation" C→T localized at the position of 775 bp of the gene encoding the CD18 subunit might be a potential QTL marker of high milk productivity. An association study between the polymorphism of SNP 775C>T, and the diversification of milk performance traits was carried out on the progeny of four bulls with genotypes BL/TL and 775C/T and one bull with genotypes TL/TL and 775C/T. The results documented statistically significant differences in the protein content percentage of milk in two half-sib families of bulls with BL/TL and 775C/T genotype and one half-sib family of bull with TL/TL and 775C/T genotype. It was further concluded that the polymorphism of SNP 775C>T was found to be a more efficient QTL marker than that of D128G, since in all the analysed milk performance traits for half-sib families higher values of the F coefficient were obtained for the SNP 775C>T mutation in comparison with D128G.

We compared the results of calf maternal nursing and cow performance in 5 beef breeds managed under equal conditions in the region of West Pomerania. The study involved 549 cows and 1 979 calves (1 007 heifers and 972 bulls) of the following breeds: Red Angus, Salers, Hereford, Limousin, and Simmental. The cows and their calves were managed outdoors for one year. Feeding and management systems were the same for all the breeds during the studied period. The calves remained with their mothers until 6 to 9 months of age. Under intensive feeding, we recorded very good calf rearing parameters with very good or good milk performance of the cows. The best results were achieved by the Simmental cows and calves. Both the heifers and calf-bulls of this breed exhibited significantly (P ≤ 0.01) higher weaning weights adjusted to 210 days of age as well as daily gains from birth to weaning. The weaning weights of the calves were higher from 37 to 93 kg and daily gains from 45 to 280 g. The Hereford cows and calves also achieved very good parameters. Relatively poorer in terms of production performance were Red Angus, as compared with the other breeds, and Limousin cows whose calves produced the lowest body weights and had the lowest daily gains as well.

Four head of Korean indigenous cattle were presented to the Chonbuk Animal Medical Centre, Chonbuk National University, with the complaint of the presence of extra limb(s) since birth. The accessory limb(s) were underdeveloped in all the cases and attached to the lateral side of the withers craniodorsal to the right scapula in Case 1 and 2; in Case 3, right dorsally to the 4^th cervical vertebra and in Case 4 it was ventrolateral to the thorax caudally to the right forelimb. In all the cases, the congenital anomaly was diagnosed as the presence of supernumerary ectopic limb(s) (SEL), which were removed by surgical excisions under general anaesthesia. In all the cases, the surgical corrections resulted in normal locomotion and better quality of life. No postoperative complications were observed during a 1-year follow up period. The SEL can be successfully removed by surgical excisions, which can result in normal locomotion and better aesthesis of the animal.

The study has been elaborated on the basis of a continuous analysis of economic results of a selected sample of agricultural enterprises operating in different climatic and productional conditions. Long-term tendencies and their influencing factors are defined on the basis of this analysis. The goal of this study is to evaluate the influence of external conditions on the economy of agricultural enterprises and on their economic results and to analyze the influence of the accession of the Czech Republic to the EU on the change of economic conditions of agricultural enterprises. On the basis of this study, the authors measures which would intensify the influence of agricultural enterprising on the rural development and which would improve the quality of life of rural inhabitants.