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Two-year data from terminal study conducted to evaluate post-weaning growth and carcass traits of Texel, Suffolk and Columbia sired offspring were used to evaluate the effect of breed on cultural energy expenditure of lamb production. Each year whiteface ewes (n = 82) composed of primarily Polypay × Dorset ewes were exposed to Texel, Suffolk or Columbia rams for 35 days in the breeding season. The ewes were wintered outdoors on average-quality lucerne hay according to NRC (1985) requirements and were not provided any concentrate during late gestation or lactation. A total of 279 lambs were born with an average of 1.7 lambs per ewe and weaned lambs were used in the study. The lambs were weaned on average at 70 days of age and lambs from each sire breed were placed either in feedlot or on pasture. Texel and Suffolk sired lambs had higher weaning weight than Columbia sired lambs (P < 0.01). Lambs in the feedlot were fed shelled maize and pelleted protein supplement. Lambs on pasture grazed for 63 days and received 455 g concentrate daily and later they were placed in the feedlot. For the cultural energy analysis, pasture establishment and maintenance, feed in feedlot and on pasture, transportation, labour, machinery, electricity and other inputs were calculated and corresponding values for each input were obtained from literature. It was assumed for the analysis that 20 percent of ewes were culled and lambs sold after weaning were included in the analysis. Texel and Columbia breeds had a higher energy input per kg live weight than Suffolk breed (P < 0.04). There was no difference between breeds in terms of energy input per kg carcass (P > 0.4). Suffolk breed had a lower cultural energy ratio for the protein energy output than Columbia and Texel breed (P < 0.02). Energy output ratio defined as kjoule input/kjoule output was better for Suffolk breed and it was different from that of Columbia and Texel breed (P < 0.03).

In the current study, intracellular (neutrophil and monocyte) concentrations of free and liposome encapsulated ampicillin in sheep were investigated. Free ampicillin (5 mg/kg b.w.) and liposome encapsulated ampicillin (5 mg/kg b.w.) were administered as a bolus intravenous injection to sheep. After the injections, blood samples (5 ml) were collected into tubes from v. jugularis at 10, 30, 60 minutes and 2, 4 and 8 hours. Neutrophils and monocytes were isolated, and lysed in distilled water. Ampicillin concentrations were measured by high performance liquid chromatography. The results indicate that liposome encapsulated ampicillin caused the higher intracellular concentrations within neutrophil (ratio of liposome encapsulated ampicillin/free ampicillin; from 1.393 to 5.416) and monocyte (ratio of liposome encapsulated ampicillin/free ampicillin; from 0.973 to 2.906) cells than free ampicillin, and liposome encapsulated ampicillin existed a longer length of time within neutrophil (4 hours) and monocyte (4 hours) cells than free ampicillin (60 minutes), as well. This formulation may be beneficial, in that the treatment of intracellular infections are caused by sensitive bacteria.

A total of 126 Salmonella spp. isolates from pigs belonging to 13 serotypes (Typhimurium, Derby, Infantis, Enteritidis, Agona, Kaapstad, London, Montevideo, Bredeney, Give, Oritamerin, Schwarzengrund and Tennessee) were tested for sensitivity to 14 antibiotics. Resistance to 1-8 antibiotics was demonstrated in 64 isolates (59.8%), classified into seven serotypes with the most frequent being Salmonella typhimurium (n = 54). S. typhimurium strains were found to be the most resistant to streptomycin (91.5%), sulphonamides (88.1%), ampicillin (86.4%), tetracycline (84.7%) and chloramphenicol (83.0%), displaying the ACSSuT phenotype. In all strains of this phenotype (n = 27), the gene for integrase (int1) and resistance genes bla_PSE-1, floR, aadA2, sul1 and tetG were detected by PCRs. In some of the strains, additional resistance to amoxycillin/clavulanic acid, sulphamethoxazole/trimethoprim, nalidixic acid and enrofloxacin was found.

Anthropogenic soils are formed by human activities. The contents of potentially risk elements are one of the most important criteria of the exploitability of such soils for the agricultural production. The aim of this paper is to assess the contents of the selected potentially risk elements in 16 areas of the Sokolov region, including 5 reclaimed areas and 11 natural soils. 116 sampling locations were analysed in total. Another aim is to analyse the relationships between the elements, using multivariate statistical methods. The contents of the risk elements studied were in most cases under the limit values. In some cases, as with Pb, they were very low compared to the limit values. In the principal component analysis, four components explaining 74% of total variability were selected. The first component (30.2% of variability) showed strong correlations with Mn, V, Ni, and Cu contents. The second component (15.8% of variability) correlated with As and Be. The third component (14.3%) correlated with Pb and Cd. The fourth component (13.7%) correlated with Zn and Cr. The mean scores of each area were projected into the component plots, which enables the assessment of the relative importance of each group of elements in each particular area. The reclaimed and the natural soils are clearly distinguished. It can be concluded that the contents of the risk elements studied do not currently present any important problem in the reclaimed areas. Nevertheless, the situation should be further monitored.

A study conducted in 1999-2000 in 6 compartments of the Chełmowa Góra forest reserve, situated in the ŚwiętokrzyskieMountains, was aimed at harmful insects infesting cones and seeds of Larix decidua Mill. subsp. polonica (Racib.) Domin. Insect rearing and seed and cone cutting yielded 1,045 specimens of insects belonging to 8 species. Resseliella skuhravyorum Skrzypcz. (Diptera: Cecidomyiidae) and Megastigmus pictus (Förster) (Hymeno-ptera: Torymidae) were the most abundantly represented species. They were present in cone samples of all investigated trees. The index of infestation of cones by insects (WZS) was higher in the case of trees growing at the forest edge. The analysis by cutting showed an insignificant percent increase - 6.7% of sound seeds while 88.9% of seeds were blind. M. pictus damaged 2.6% of larch seeds, while R. skuhravyorum 1.7%. The parasitoid Mesopolobus zetterstedtii (Dalla Tore) was received from the mass rearing where its host is M. pictus.

Damage to forest stands and their decline in the Czech Republic evoked a necessity to elucidate the function of soil in these processes. Main factors affecting acidification of forest soils are assessed in the paper. On the one hand, it refers to natural factors such as properties of soil-forming substrates and species composition of forest stands, on the other hand, to the effects of anthropogenic character such as influences of man-made spruce monocultures and effects of acid depositions. Special attention is paid to the development of forest soils in the Krušné hory Mts., where the effects of acid depositions are highest.

This paper follows previous papers published by the authors. All the papers are concerned with standard direct payments, the system of administration of direct payments approved for 2004 and their impact on the economy of agricultural enterprises farming in different production areas. The impact of different ways of using additional payments for the years 2005 and 2006 are compared in this report. Attention is mostly paid to ways of the maximum adaptation to the Reform of Common Agricultural Policy.

Hungary has recently become a member of the European Economic Union (EU) and most of the economic benefits are expected to come from expanded trade with other EU nations. While some variation in agricultural policy continues to exist between EU members, all countries generally, benefit from lower tariffs and expanded trade opportunities. However, Hungary must also be able to compete on the basis of quality and price in order to maintain current domestic markets and sell more to other EU countries. In order for the Hungarian agriculture and food industry to contribute to economic development it must continue to focus on efficiency and competitiveness. Hungary benefits from many natural features, which provide favourable conditions for agriculture: fertile plains, an advantageous climate and production experience, which makes possible a total yearly agricultural and food products trade surplus fluctuating between 1.5 and 2 billion US $ for the last 12 years. However, after the EU accession, the Hungarian internal market has become fully open and domestic products have to compete with the products of other EU members. This is why the renewal of food regulation and policy was indispensable. This article examines the Hungarian food policy (1) before the transformation to a market oriented system, when the policy was quantity orientated, (2) during the pre-accession period, when quality policy became more important, and (3) after accession to the EU where food safety has become more important.

An improved protocol for Brassica protoplast culture and plant regeneration was developed. Isolated protoplasts from four-weeks-old in vitro shoot tip culture of Brassica oleracea var. botrytis cv. Siria F1 and Brassica napus doubled haploid of breeding line OP-1 were cultured at a density of 9.8-11.2 × 10⁴ protoplasts/ml in darkness at 25°C in a modified medium containing 2% glucose, 0.25 mg/l 2,4-D, 1 mg/l BAP and 1 mg/l NAA. The first divisions of protoplasts were observed on the third day of culture in B. oleracea and on the fourth day in B. napus. The protoplast cultures were diluted with low osmotic medium on 7^th and 11^th day. The frequency of dividing cells was about 80% in B. oleracea and 50% in B. napus. After one month, the microcalli of approximately 0.5-1 mm in size were transferred into an induction medium with various combinations of growth regulators. Minimum duration of enzyme treatment time and extended dark period in the initial phase of culture increased the survival rate of protoplasts. Organogenesis started when the calli enlarged in size on an induction medium (1 mg/l NAA, 0.02 mg/l GA₃, 1 mg/l 2iP) with 2% sucrose and 0.8% agar. Regeneration frequency of calli was found to be 69-75% in B. oleracea and 2-3% in B. napus. Well-developed shoots were transferred for rooting to a half-strength MS medium without growth regulators. More than 100 B. oleracea regenerants were transferred into soil, and they produced normal heads and set seeds. This very simple procedure is efficient and suitable mainly for B. oleracea var. botrytis and represents a background for fusion experiments.

The aim of study was to verify a possibility of adding boron to multi-nutrient soil tests: Mehlich 3, water (1:5) and 0.5M NH₄-acetate extraction. Thirty-six different soils from topsoils of agriculturally farmed field were used for the study. The basic set of 36 soils was doubled when the same soil samples with gypsum addition at an equivalent dose 2 t Ca/ha were used. The closest correspondence between B in soil (n = 72) and B in plant was found out by the NH₄-acetate soil test. This test proved its good universality and independence on a radical intervention in soil chemistry by gypsum treatment. It responded to an increase in B-availability to plants after gypsum treatment of soil. The adjustment of NH₄-acetate boron by the percentage difference between the actual and the desired pH of soil improved the closeness of the correlation. Although the H₂O soil test showed its lower universality, it is assumed to use the H₂O soil test for prediction of B-status in soil when the history of previous fertilisation of the field will be known. The Mehlich 3 soil test did not prove to be suitable for diagnostics of B-status in soil.

A total of 1 200 broiler breeder eggs were collected from a commercial flock at the hen age of 37 weeks and divided into storage treatments of 5 and 15 days. Prior to storage, the eggs were further divided into pre-storage incubation (PRESI) 0 (control), 4 and 8 h treatments. Eggs in the 4 and 8 h PRESI treatment were incubated at a standard dry-bulb temperature of 38.0°C. All eggs were weighed prior to and after storage, then incubated in a commercial setter and hatcher for 21 days. After the incubation, all unhatched eggs were opened to determine fertility, hatchability and embryonic death. The 4 and 8 h PRESI treatment significantly decreased hatchability results of long-term stored eggs compared to non-heated eggs. Hatchability results of eggs stored for 5 days were significantly better compared to eggs stored for 15 days. Subsequent growth performance of progeny was not significantly affected except for the main effect of egg storage on feed conversion. There were significant PRESI × egg storage interactions for apparent fertility, hatchability of total and fertile eggs and embryonic mortality. It was concluded that the PRESI treatment did not have a detrimental effect on the hatchability of broiler breeder eggs stored for 5 days and it might even increase hatchability while it had a detrimental effect on the hatchability of broiler breeder eggs stored for 15 days.

Live body measurements of weight, height, length, girth, stifle- and hip-width and a measure of muscularity (ratio of stifle to hip width) were monitored on 258 Yankasa sheep stratified into age categories of 1 to over 3 years determined mostly from records and partly by dentition. These animals are from purebred Yankasa sheep kept as a part of the open nucleus-breeding scheme of the National Animal Production Research Institute, Shika, Zaria, Nigeria. The effect of sex, type of birth and age group of lambs on live measurements and muscularity were analyzed by least-squares procedures. All variables examined, except sex, had significant (P < 0.001) effects on all body measurements. At birth, male and single-born lambs were significantly heavier than female and twin-born lambs. At early age, most factors significantly (P < 0.01) influenced the body weight, body dimensions and muscularity traits with single-born lambs being 31% heavier, 10% taller and 11% bigger in girth; more highly muscled than the twin-born lambs. This advantage of male over female born lambs (except in hip-width and girth) for growth and developmental traits was maintained reasonably well to the advanced ages. All phenotypic correlations between body measurements were positive and significant (P < 0.001). The highest correlation coefficient was found between chest girth and body weight. The polynomial equation using chest girth as an independent variable predicted body weight more accurately as compared to the linear equation.

The present study was designed to compare the efficiency of bovine embryo production for cryopreservation between oocytes collected from donors in the growth phase of follicular development (GPFD) and those recovered from donors in the undefined phase (UPFD). Cyclic cows, Czech Siemental or Holstein dairy breeds, 4-6 years of age, slaughtered at the local abbatoir were used. They were divided into two groups based on ovarian morphology: I. GPFD donors with ovaries corresponding to the growth phase of the first follicular wave (estrus cycle days 3-4; n = 52), and II. UPFD donors with ovaries in any other phase of follicular development (undefined estrus cycle days; n = 89). A total of 3 771 oocytes were collected and 1 134 embryos were prepared as two separate populations by standard protocol. In total 352 excellent or good quality embryos at the early, advanced or expanded blastocyst stage from both donor groups were pooled and used for cryotolerance assessment. They were frozen on day 7 (D7) or day 8 (D8) after fertilization by the standard procedure. After thawing, the embryos were cultured for 72 h to the hatching stage. The percentages of both D7 embryos and advanced blastocysts were significantly higher (P ≤ 0.01) for oocytes collected from GPFD donors than for oocytes collected from UPFD donors (33.7 vs 28.6% and 43.0 vs 29.5%, respectively). The percentages of excellent or good quality embryos obtained from both D7 embryos and fertilized oocytes were significantly higher (P ≤ 0.01) for oocytes collected from GPFD donors than for oocytes collected from UPFD donors (63.6 vs 49.4% and 21.4 vs 14.1%, respectively). The post-thaw survival rates were significantly higher (P ≤ 0.01) for D7 than D8 embryos (80.4 vs 66.3%). In relation to the developmental stage, the development and hatching rates were significantly higher (P ≤ 0.01) for D7 than D8 early blastocysts (75.0 vs 41.2% and 50.0 vs 5.9%, respectively) and for D7 than D8 advanced blastocysts, (73.7 vs 57.1 and 52.6 vs 28.6%, respectively). No differences were found between D7 and D8 expanded blastocysts after freezing-thawing. In conclusion, the collection of oocytes from donors in the growth phase positively influenced the in vitro production of bovine embryos for cryopreservation. The development of embryos produced with oocytes from GPFD donor group was accelerated and more excellent and good advanced blastocysts with greater cryotolerance were produced on day 7 after fertilization, as compared to embryos produced with oocytes from the UPFD donor group.

In this study, the compatibility of the pear cultivars PI1/6 and BC Williams with 49 quince genotypes was evaluated. The results showed that the cv. PI1/6 had a good compatibility with several of the quince genotypes, and the cv. BC Williams only with a few of them. Differences were also found in their susceptibility to fire blight (Erwinia amylovora). The cultivar PI1/6 was very susceptible, while the cultivar BC Williams was relatively tolerant.

This study deals with the assessment of increment losses in Scots pine trees caused by the maturation feeding of pine shoot beetles Tomicus piniperda (L.) and T. minor (Hart.) (Col., Scolytidae) in even-aged stands growing during their entire life span within range of the influence of a sawmill and its timber yard. In spring 2004, on three sample plots, 0.2 ha in size, situated 60, 200, and 500 m from the yard, height and dbh of all trees were measured and increment cores were taken from randomly selected sample trees. An agreement between the spatial distribution of losses in tree increments and the distribution of damage to crowns of investigated stands indicated that the losses resulted from the maturation feeding of pine shoot beetles migrating from the sawmill timber yard. Divergence of growth in the about 25-years-old stand indicated the beginning of intensive feeding of beetles in pine shoots with a high level of probability. In comparison with the control stand the basal area losses in stands growing 60 and 200 m from the beetle source amounted to 57% and 46%, respectively. The difference in the height of trees was as large as almost 100%.

In the years 1995-2004 the spectra of weeds, diseases and pests in grasses grown for seed in the Czech Republic were examined and the effect of external factors on their changes was assessed. The species spectrum of weeds evaluated both at the time of vegetation and in samples of natural seeds was stable throughout the period and the changes in the number of individual species were caused by different proportions of grass species and stand age. The most abundant weeds were Elytrigia repens, Matricaria sp.div., Anthemis sp.div., Apera spica-venti, Cirsium arvense, Poa trivialis, Rumex sp.div. and Chenopodium sp.div. The most serious diseases were parasitic silvertop, graminicolous rusts (Puccinia sp.div.), Blumeria graminis and ergot (Claviceps purpurea). The rate of rust occurrence was affected by climatic conditions; the spectrum of other diseases was stable throughout this period. The most important animal pests were Muridae rodents.

Fertilization with tannery materials positively influenced the soil pH and increased the content of alkaline cations in soil. After four years of investigations organic carbon content decreased most in the soil fertilized with untreated sludge, mineral salts and in the control soil. Irrespective of fertilization used and year of studies cadmium occurred mainly in most mobile fractions (mobile, exchangeable and bound to manganese oxides). Copper was mainly bound in fractions less available to plants. After four years of studies an increase in mobile copper forms was registered in all treatment soils. Lead in the soil was present mainly in the form bound to organic matter and amorphous iron oxides. Distribution of lead points to the small share of this metal in the mobile and exchangeable forms, both immediately after fertilization applied and after four years of studies. Chromium became fixed in fractions less available to plants, mainly bound to iron oxides and in residual fraction. On the other hand, an apparent effect of fertilization with materials of tannery origin on this element total forms content was registered.

This study compares effects of 2,3,5-triiodobenzoic acid (TIBA) and flavonoid quercetine (Q). In spite of the fact that both these compounds are inhibitors of the polar transport of auxin, a number of experiments demonstrated that their properties are different. If the flax seedlings were decapitated and one cotyledon was removed, the axillary bud of the remaining cotyledon grew up more intensively while TIBA (0.5% applied in the form of a lanolin paste on the remaining cotyledon) induced a correlative reversal so that axillars of removed cotyledons grew up more intensively. However, when Q (0.5%) was applied on the remaining cotyledon in the form of a lanolin paste, this inhibition was not significant. In the lamina-deprived petioles of apple trees TIBA accelerated abscission while Q caused an inhibiting effect similar to that of auxin. TIBA applied on epicotyls of pea seedlings inhibited their growth by 35% while Q only by 15%. As far as the release of ethylene by pea seedlings is concerned, both compounds showed promoting effects similar to the effect of auxin.

The objectives of the paper were to evaluate carcass value in reared gilts on the basis of carcass analysis during the station test of purebred progeny of pigs and ultrasonically with Sonomark-100 or Piglog-105 instruments in the framework of valid methodology for performance testing, and to verify a pos-sibility of prediction of backfat thickness and/or average daily weight gain since birth and lean meat content of gilts as the elements of objectification for the evaluation of their body condition. In total 54 gilts of two breeds were evaluated: the dam breed Czech Large White and the sire breed Czech Large White - sire line. Four-parameter Richards function was used for growth evaluation. The regression function y = 63.870 - 0.447 bt₁ - 0.510 bt₂ + 0.128 MLLT was applied in ultrasonic instruments for the calculation of lean meat content. We calculated linear regression functions for the conversion of performance testing parameters (gain from birth, average backfat thickness and lean meat content) from live weight on the day of measurement per live weight declared during selections in gilts.

The objective of the experiment was to evaluate the effect of starter diet containing 10% of treated rape cake (TRC) with a reduced glucosinolate content on calf growth, development and health. The experiment included 45 calves allocated into 3 groups. After the colostrum period (5 days) the calves were fed 4 l of pooled fresh milk per calf/day and one of the starters ad libitum. The control group (S) was fed Standard starter with components with a standard nutrient content including fibre. Comparative group T was fed Telstar starter, which contained whole maize kernels and protein pellets. Experimental group G was fed Grant starter containing 10% of treated rape cake. The calves were fed hay ad libitum from 10 weeks of age. The average starter consumption (calf/day) over a 90-day feeding period was 1.34, 1.72 and 1.45 kg of the Standard, Telstar and Grant diet, respectively. An insignificantly higher body weight of calves was recorded for the Grant group at the end of the experimental period. When the calculated indices were compared, it was obvious that the weight gains obtained with experimental starters Grant and comparative Telstar were by 4.0% higher and by 5.4% lower, respectively, than in the control group Standard. No significant changes in the parameters of nitrogen, energy, lipid and mineral metabolism, health status, hepatic parenchyma and thyroid function were observed throughout the experimental period. The diet based on experimental starter Grant had no adverse effect on the ruminal fermentation in calves. It may therefore be concluded that the starter diet with 10% content of treated rape cake had no adverse effect on calf growth and performance.

In 16 male dogs who suffered from perineal hernia, polypropylene mesh was used to close a defect in the pelvic diaphragm. Pelvic bone was drilled on the pelvic floor and mesh was sutured through holes by polypropylene suture. Strong pelvic diaphragm, good long-term results and time-sparing by this technique was achieved. Suture sinuses were developed in two dogs one month postoperatively. Objectives of this study were to describe a new alternative technique of perineal herniorraphy and postoperative possible complications. Weakness of internal obturator muscle flap is complication which can be observed during transposition of internal obturator muscle flap. This technique can be used when internal obturator muscle flap is weak like the operation of the first choice.

An outbreak of tuberculosis among bovines and pigs caused by Mycobacterium caprae is described in this paper. After tuberculin skin tests with bovine purified protein derivates (PPD) six cattle and one sow, own by a small family farm, tested positive whilst three pigs were suspected in 2004. All animals were euthanised and checked for gross pathological lesions. Generalised lesions were found in five cattle and two sows; however one calf and two gilts had lesions that were localised in the submandibular lymph nodes. Mycobacteria were isolated from tissue samples of six cattle and four pigs. Mycobacterial isolates were identified using classical biochemical tests and molecular methods (PCR, GenoType MTBC) as M. caprae. Mycobacterial Interspersed Repetitive Unit (MIRU) typing of isolated mycobacteria showed an identical number of repeats in 12 different loci. Results of the research confirmed the domination of M. caprae among infected cattle in Croatia; however this paper was the first to confirm a case of M. caprae in pigs. The source of the infection was not found.

The condition was evaluated of tissues of declining young spruce stands in selected localities of the Orlické hory Mts. It refers to stands occurring in top parts affected by an air pollution disaster in the last century. Several types of damage of a different symptomatic character were distinguished. A high NO_x input appears to be the risk factor of spruce dieback in the Orlické hory Mts. One of the main causes in the complex of factors is the unbalanced cause of meteorological factors during the year and unbalance in mineral nutrition. The result is decrease in lignification, immaturity of tissues before the winter period and increased susceptibility of damaged tissues to parasites. Several potentially pathogenic species of micromycetes have been identified which can be considered to be significant pathogenic agents in case of declining spruce in the Orlické hory Mts., however, not a main cause.

Dokumenty týkajúce sa regulácie hospodárenia v lesoch sa prakticky až dodnes považovali za materiály, týkajúce sa skoro výlučne len jednotlivých odborných rezortov. Na základe Vyhlášky Ministerstva financií SR č. 465/1991 ich možno efektívne využívať aj v rámci rezortov financií a spravodlivosti. Ďalšie významné medzirezortné využitie týchto dokumentov vyplýva zo Zákona č. 127/1994 o posudzovaní environmentálneho dopadu. Cieľom práce je demonštrovať proces posudzovania na konkrétnom príklade - ploche patriacej do lesného pozemkového fondu. Okrem máp, výsledkov pozorovania, slovných popisov a súborov numerických údajov, týkajúcich sa jednotlivých porastov, obsahujú tieto dokumenty aj kódované údaje, ktoré pre väčšinu užívateľov nie sú zrozumiteľné bez využitia dekódovacích kľúčov. A práve tieto posledné údaje majú pri vyhodnocovaní environmentálneho dopadu najvyššiu výpovednú hodnotu. Po nadobudnutí platnosti pripravovaného zákona (vyhlášky) o strate vlastníctva, vyplývajúcej z prohibitívnych a ďalších podmienok v lesnom hospodárstve v súvislosti s presadzovaním verejno-prospešného záujmu (Kundrík et al. 1999), významne vzrastie dôležitosť využitia finálnych dokumentov týkajúcich sa regulácie hospodárenia v lesoch SR.

The investigations were conducted in the frame of EU Research project Wood for Energy - a Contribution to the Development of Sustainable Forest Management (2001-2005). The integrated wood ash experiment was set up in a 38-year-old Scots pine (Pinus sylvestris L.) stand on Arenosols in SW part of Lithuania. Raw (not hardened) wood ash and nitrogen fertilizers were applied in 6 variants: 1.25 t ash/ha; 2.5 t ash/ha; 5.0 t ash/ha; 180 kg N/ha; 2.5 t ash + 180 kg N/ha and control (no treatment). The changes of soil pH, the content of some nutrients, heavy metals in Arenosols and soil solution, the abundance of ammonifiers, nitrifiers and denitrifiers in forest floor and mineral topsoil after the application of wood ash are presented and discussed in this paper.

The European integration process is very important and it has been paid attention to for the last 15 years. The abstract deals with the negotiation field and position in the structure of the current expanded EU. For better orientation in this equivocal situation, a modern cartography method of relationship in the arbitrary group called dynamic sociometry was used. The method is based on classical sociometry Morena and furthermore it uses the instrument of fuzzy set, typology and structural analysis. The output of this method is a sociomap. The map holds information on the relative closeness or the distance of individual elements, their configuration but also some quality information. The graphic chart is similar to a topographic map. In our case, the sociomap was created from the data of foreign business among the member countries. The following analysis of the sociomap we detected and described characteristic features of the analysed group. It consists of formal and informal links in the group, the role and the position of each member within the group, the structure and relations in the group. In the concrete, we attained data to answer the questions about the present climate in interrelationships in the EU, which means the relationships among the members as the whole entities but also the relationships separately among members themselves. The position analysis of the Czech Republic in the system of the created sociomaps was considered as very important. According to the allocation of the Czech Republic in sociomaps, we easily defined the position of the state, the closest partners for negotiation, and also the possible problem relations which might complicate the negotiation in the EU. From the resultant sociomaps, we also tried to determine the sociometric star, i.e. the leader of the European Community. We anticipated that it would be rather an aggregation of several states than an individual state. The contribution focuses on the negotiation field and position in the structure of the current expanded EU. The sociomap created using foreign business data of member states gives the bases for answering questions about present climate in interrelationships in the EU which means the relationships between the members as whole entities but also the relationships separately between the members themselves.

During 4 years, 27 cultivars and breeding lines of winter wheat (Triticum aestivum) were tested in small plot experiments for resistance to powdery mildew fungus. The most resistant were Frimegu, RE9607, Runal, Asset, Folke and Wasmo. The cultivars Asta (Pm2,6) and Vlasta (Pm2,6 and another not determined specific gene or minor genes of resistance) fall into resistant cultivars. It seems that the specific genes of resistance Pm2 and Pm6 are still very effective against the present Czech population of powdery mildew on wheat. Resistance of the cultivars Hereward and Tarso, having the gene of resistance Pm8, can be ascribed to an additional undetermined gene that is effective only in mature plants. The cultivars Mikon and Ramiro with partial resistance had a higher infection type and disease severity than resistant cultivars, but lower disease severity than the susceptible cultivar Kanzler.

Pea seeds were swollen in solutions with different concentrations of cytokinin benzyladenine (BA). As compared with controls, a low concentration of BA (0.05 mg/l) did not affect the growth of stems and roots of twelve-day-old seedlings but concentrations of 0.20-4.00 mg/l showed an inhibiting effect; this inhibition was proportional to the concentration of BA. In controls the ratio between the lengths of stems and roots was equal to 1.14, while in seedlings influenced by BA in concentration of 0.20 mg/l its value was significantly lowered to 0.91. Also in six-day-old pea seedlings cultivated under in vitro conditions, BA concentrations of 0.70 and 3.30 mg/l inhibited the growth of roots and epicotyls and significantly increased the production of ethylene (by 150 and 330%, respectively). Three hours after the amputation of the root of five-day-old pea seedlings the level of cytokinin trans-zeatin increased in the apical part of the stem. Within the interval of 48 hours after the amputation of the root the concentration of this plant hormone gradually decreased again. The initial increase in the zeatin level in the stem indicates that the absence of the root induces a reduction of growth-inhibiting effects of roots, which negatively influences the zeatin level in the stem apex at the beginning of germination.