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An 8-year-old, 6.5 kg, neutered female Shih-Tzu dog was presented for surgical resection of a mediastinal mass. A median sternotomy and left cranial lung lobectomy were performed. Intraoperatively, with the patient under general anaesthesia, a bilateral transversus thoracis plane (TTP) block was performed by injecting 0.5% bupivacaine (0.2 ml/kg) per side using real-time ultrasound guidance. After surgery, indwelling catheters for repeated bolus injections of bupivacaine in TTP were placed as follows: the fifth sternebra was palpated in dorsal recumbency, and the transducer was placed in the longitudinal plane lateral to the sternal border. A 16 gauge over-the-needle catheter was inserted caudo-cranially using an in-plane technique and located in the TTP. An intermittent bolus of bupivacaine (0.1 ml/kg) per side was injected via the indwelling catheter every 8 h for 3 days, with a constant rate infusion of an intravenous fentanyl (1 μg/kg/h) and ketamine (0.12 mg/kg/h) combination. Post-operative pain was evaluated using the Glasgow composite measure pain scale and the score was 4–5/24 on the day of surgery and gradually decreased over time. Additional rescue analgesia was not required. Repeated boluses of bupivacaine for a continuous bilateral TTP block may be a useful adjuvant for perioperative pain management strategies, including median sternotomy, in dogs.

This study was planned to investigate an alternative treatment modality in diabetic wound healing. In this experimental study, the efficacy of both cold atmospheric plasma/nitric oxide (NO) and NPH insulin ointment, recently known to have beneficial effects on wound healing, was investigated in diabetic wound healing. Twenty-four (24) diabetic rats were divided into four groups DC, DI, DNO and DINO (diabetic control, diabetic insulin, diabetic nitric oxide, diabetic insulin + nitric oxide groups). No treatment was applied to the DC group, NPH insulin was applied to the DI group, CAP/NO was applied to the DNO group, and CAP/NO + NPH insulin was applied to the DINO group once daily for 14 days. The wound area reduction and the wound contraction rate were calculated on the basis of the tissue sections taken, and histopathological and genetic analyses were carried out. Compared to the control group, exogenous NO gas was found to be a potent antibacterial agent in the diabetic wound healing, causing a reduction in the wound area (P = 0.034), an increased contraction rate (P = 0.021), epithelialisation (P = 0.02), collagen organisation (P = 0.006) and a reduction in the number of inflammatory cells (P = 0.002). A significant increase in the expression of IL-8 mRNA was observed (P = 0.026). It was concluded that NPH insulin alone contributes to wound healing, but it is not necessary to use it together with exogenous NO gas.

Poplar species such as aspen (Populus tremula L.) play a very important role in the forest formation process not only in Eastern European regions. Unfortunately, such aspen stands are often severely affected by fungal diseases, causing mainly core rot. In this study, the indirect effects of thinning on the phytosanitary condition of aspen by promotion of tree growth were investigated. Two thinning methods, manual (thinning from below) and mechanical thinning (schematic), were applied to young stands dominated by Eurasian aspen to study their effects on tree growth and health. All trees were measured at breast height and diameter frequency distribution was determined twice, i.e. three and 24 years after the beginning of the experiment. In addition, during the second measurement, tree-ring samples were obtained from individual trees to evaluate growth and wood decay damage. Neither manual nor mechanical thinning of aspen significantly increased its growth at the stand level, but positive effects on individual trees were observed in plots where mechanical thinning was applied. The thicker the trees, the less decayed they were. The analysis suggests that thinning in general should not be used to increase stand production, but the positive effects of mechanical thinning on individual aspens can be recommended to promote the growth of individual vigorous trees.

The aim of the study was to evaluate the effect of feeding regime (FR) and the combination of FR with pasture on the carcass composition and meat quality parameters of fast-growing chickens. Ross 308 chickens were split into three groups: Group 1 was fed ad libitum, and Groups 2 and 3 had a restricted diet. The chickens were feed-restricted at a rate of 70% ad libitum from 8 to 14 days of age. In Group 3, after restriction at the age of 21 days, chickens were kept on a pasture until the end of the experiment at 35 days of age. The chickens were fed ad libitum prior to and following restriction. Feed restriction and the combination of feed restriction and pasture significantly reduced final body weight, but the dressing out percentage was not affected. The breast percentage was the highest (P = 0.005) in the ad libitum group (30.5%), followed by the restricted group (28.2%) and the lowest in the group with a combination of feed restriction and pasture (27.4%). Breast pH and colour measured 24 h post mortem were not affected, whereas texture expressed as F_max was the lowest in the group with the combination of feed restriction and pasture (P = 0.05). There was no effect of the group on meat dry matter, crude protein, cholesterol, and fatty acid content, but ether extract was the highest, and significantly so, in the ad libitum-fed group. In summary, feed restriction and the combination of feed restriction and pasture negatively affected final body weight and breast and abdominal fat percentages, which might be related to a short realimentation period for compensatory growth. However, these conditions negligibly affected carcass composition and the physical and chemical parameters of the meat.

Alfalfa (Medicago sativa L.) is a superior-quality perennial legume forage crop cultivated in China. However, fertiliser applications and the environmental factors affecting alfalfa yield and quality have not been well documented. In this study, we conducted a meta-analysis using a dataset from 105 studies published between 2003 and 2023 to explore the effects of fertiliser application and environmental factors on the yield and quality of alfalfa. The results showed that compared to the non-fertiliser control levels, fertiliser application increased alfalfa yield by 24.61% and improved the quality of alfalfa by increasing crude protein by 11.63% and decreasing acid detergent fibre by 7.69% and neutral detergent fibre by 6.76%. Alfalfa yield and the crude protein effect size increased with increasing altitude but decreased with increasing latitude based on fertiliser application. The acid detergent fibre and neutral detergent fibre effect size were positively correlated with mean annual temperature and mean annual precipitation. In conclusion, applying fertiliser is a productive approach to enhance the yield and grade of alfalfa, but environmental factors have an effect. This study provides comprehensive information on fertiliser applications and environmental factors that affect alfalfa yield and quality. These results provide insight into further improving alfalfa yield and quality and contribute to the development of alfalfa.

Amino acids have positive regulatory effects on the function of intestinal epithelial cells (IEC), but in the field of animal nutrition, research on the regulatory effects of amino acids on IEC is still in the initial stages. This study aims to explore the effects of Gly, Gly-Gly, and Gly-Leu on IEC proliferation and their possible mechanisms. Chicken small intestinal epithelial cells were separated using the tissue block method, and other miscellaneous cells were removed for digestion and passage culture. The IEC were cultured in the medium containing 20 nmol/l Gly, Gly-Gly and Gly-Leu for 24 h, and the expression of enterokinase and cytokeratin in cells, the growth curve and activity of IEC, cell cycle, differentially expressed genes, mRNA expression, and protein expression levels of p-mTOR and p-S6K1 in IEC were detected. Enterokinase and cytokeratin were expressed specifically in IEC. The results of growth curve and MTT revealed that the cell viability of IEC was significantly increased after treatment with Gly, Gly-Gly and Gly-Leu. The cell cycle results showed that compared with the control group, Gly, Gly-Gly and Gly-Leu intervention could increase the proportion of IEC in G1 phase, and the proportion in S phase of IEC was decreased. Transcriptome sequencing showed that compared with the control group, there were 54, 28 and 30 differential genes in Gly group, Gly-Gly group and Gly-Leu group, respectively. These genes were mainly enriched in nitric oxide synthesis and protein kinase B signalling, PI3K signal and cellular amino acid biosynthesis and transport signal pathways. RT-PCR results showed that the mRNA expression levels of PCYT2, SPP1, EMC6, GRIA2 and PKD2 were consistent with the sequencing results. Western blot results showed that compared with the control group, the protein expression of p-mTOR and p-S6K1 in Gly group, Gly-Gly group and Gly-Leu group was significantly increased. Gly-Leu can promote the protein synthesis in IEC by activating protein synthesis of mTOR signalling pathway in chicken IEC.

Several antimitotic agents were tested in three embryogenic doubled haploid (DH) lines of swede (Brassica napus ssp. napobrassica). No effect on embryogenesis was observed at the given concentrations of colchicine (5 μmol/L or 50 mg/L) and treatment time of 24 hours. Flow cytometric analysis of microspore embryos revealed a significant increase in the percentage of DH compared to the control for all substances: ethalfluralin, 62.2%; trifluralin, 58.3%; colchicine, 56.1%; amiprophos-methyl, 54.4%; pronamide, 35.0%; control, 13.3%. The occurrence of pure tetraploids was low (0–5%), mixoploids – especially n+2n – were high in all treatments, including the control (29.4–71.1%). Their presence can be explained as a consequence of spontaneous diploidisation and/or endoreduplication during embryogenesis or embryo germination.

The effectiveness of pheromone lures for Grapholita molesta (Busck 1916) from Propher (Czech Republic) and Pherobank (Netherlands), the attractiveness of green and transparent pheromone traps and the representation of non-target species were evaluated in three study areas of southern Moravia (Czech Republic) in 2019–2021 (24 traps per year). A total of 6 536 Grapholita molesta and 946 individuals of 17 non-target species were collected. Grapholita molesta was frequently captured in green delta traps with a Pherobank pheromone lure. Therefore, pheromone lures from different manufacturers have different efficacy and require the independent setting of harmfulness thresholds. An overview of non-target species recorded by other authors was compiled. Among the non-target species, morphologically similar species of Tortricidae dominated, causing considerable problems in the signalling of control measures of the target species.

Cadmium is one of the most important environmental contaminants. Animals grazing on natural pastures are particularly exposed to cadmium. Sheep are mostly reared in extensive and grazing systems. Therefore, sheep may accumulate larger amounts of cadmium compared to other ruminant species and are a good indicator of exposure to cadmium contamination in a given area. The determination of cadmium concentrations in the muscles, livers and kidneys of lambs and sheep was carried out in the Czech Republic during the period 2001–2022. The average cadmium content in the livers and kidneys of all the lambs was 0.037 ± 0.006 and 0.061 ± 0.013 mg.kg^–1, respectively. The average cadmium content in the livers and kidneys of all the sheep was 0.319 ± 0.047 and 1.255 ± 0.204 mg.kg^–1, respectively. The maximum limit for human consumption was not exceeded in any of the 24 samples in the lambs but was exceeded in 5 of 33 liver samples and 12 of 33 kidney samples in the adult sheep. The average value of cadmium in the muscles of the lambs and sheep was 0.004 ± 0.001 mg.kg^–1. The maximum limit for human consumption was not exceeded in any muscle sample. The cadmium content in both the liver and in the kidney differs significantly between the lambs and sheep, being higher in the sheep (P < 0.001 for both comparisons). No statistically significant trend of a decrease in the cadmium content in the lamb and sheep tissues was found during the observed time (P > 0.05). We can sum up that there is an evident need for further monitoring of the cadmium concentration in lamb and sheep tissues in the Czech Republic.

Despite the routine use of cryopreservation in dairy cattle, the subsequent deterioration in the quality of bull insemination doses (IDs) is an incentive to refine cryopreservation protocols, including the composition of the ID. The aim of this study was to examine, using a flow cytometry assay, whether the selected concentrations of low density lipoproteins (LDLs) extracted from hen-egg yolk may improve the post-thaw quality of the ID diluted with a plant-based extender. In total, 30 ejaculates from five sires (six trials per animal) were collected and processed at the Artificial Insemination Centre. The effect of the low density lipoprotein supplementation to the AndroMed^® diluent at 4 and 8% (v/v) on the cryopreservation efficiency was tested. The effects of the modified extenders were determined on different sperm subpopulations by simultaneous quadruple staining for flow cytometry as the percentage of sperm with intact plasma membrane and acrosome (PMAI), PMAI sperm showing high mitochondrial membrane potential (HMMP), sperm with plasma membrane damage (PMD), and sperm with acrosome damage (AD). It was observed that the 8% LDL treatment was more effective (P < 0.05) in preserving all the analysed parameters than AndroMed^® without any supplementation. The supplementation of the AndroMed^® extender with 8% LDL resulted in the most optimal values of the PMAI (30.61 ± 1.13), HMMP (68.81 ± 1.25), PMD (68.69 ± 1.14), and AD (38.36 ± 1.13) compared to the control treatment (24.86 ± 1.13 for PMAI, 47.79 ± 1.25 for HMMP, 74.77 ± 1.14 for PMD, and 42.83 ± 1.13 for AD. The results of the study also demonstrated a synergistic positive effect of the LDL and soybean lecithin-based diluent on the spermatozoa post-thaw quality and resilience, based on 2 h long incubation. In conclusion, the soybean based semen extender treatment with LDL represents a beneficial tool to mitigate the detrimental effect of cryopreservation.

Anthocyanins have received an increased attention not only because of its antioxidant activity; but because fortification of food products by minerals is important due to the lack of some minerals in population. The addition of these minerals can affect the sensorial and nutritional composition of food. The influence of calcium fortification on anthocyanins and colour changes in strawberry puree were assessed by accelerated storage test. The quantification of anthocyanins was performed by high-performance liquid chromatography with diode-array detection (HPLC-DAD) and colour changes were measured spectrophotometrically (CIE L* – lightness, a* – redness, b* – yellowness). The kinetical parameters (velocity constants and activation energies) were calculated. The activation energies of degradation of anthocyanins were calculated as pelargonidin-3-glucoside (26.24 ± 0.57, 21.18 ± 1.07, and 24.53 ± 1.33 kJ·mol^–1), cyanidin-3-glucoside (16.10 ± 0.96, 11.61 ± 0.74, and 13.34 ± 1.72 kJ·mol^–1), and pelargonidin-3-rutinoside (8.91 ± 0.17, 7.39 ± 0.98, and 8.23 ± 1.72 kJ·mol^–1) of the control sample, calcium carbonate and calcium citrate respectively. The results showed that the addition of calcium salt had a statistically significant (P ≤ 0.05) effect on the degradation of anthocyanins.

Wheat leaf rust is a devastating disease worldwide. Identification of leaf rust resistance genes in seedlings and of genes for slow rusting are important in resistance breeding and for gene deployment to control the disease. A total of 108 wheat cultivars from the Beijing and Shandong province and a set of 36 differentials, mostly near-isogenic lines in the background of Thatcher with known leaf rust resistance genes, were tested with 20 Puccinia triticina pathotypes  (FHJS1, FGBQ,PGJQ, SHJT, FHGQ, PHTT1, FHGQ, FHGQ, PHJS, THSM, FHSQ, PHST, PRSQ, FNTQ, PHGM, KHGQ, PHTT2, TGTT, FHJS2, NHHT) at the seedling stage in the greenhouse. The cultivars and differentials were also planted in the field to test their slow rusting resistance using a mixture of races at Baoding, Hebei province and Zhoukou, Henan province, for two consecutive years. Ten leaf rust resistance genes, Lr1, 9, 10, 19, 20, 24, 26, 34, 37 and 46 were identified  in the 57 commercial wheat cultivars, either singly or in combination, using molecular markers. Combined, the results from gene postulation and marker detection showed that one or more of the genes Lr1, 3, 10, 14a, 14b, 26, 36, 39, 34 and 46, were present in 57 cultivars, and that no known resistance gene was present in the remaining 51 cultivars. The resistance gene Lr26 was present in 42 cultivars, and nine cultivars contained Lr1. Lr46 was present in 10 cultivars, as indicated by the presence of the closely linked marker csLV46G22. Seven genotypes were identified as possibly carrying the gene Lr39. Lr3 and 10 were found in six and four genotypes, respectively. The ggenes Lr14b and 34 were each present in three cultivars, while evidence for the presence of Lr14a and 36 was obtained in single genotypes. Finally, 12 cultivars showed slow rusting resistance at two locations in two crop seasons. The identification of leaf rust resistance genes in Chinese wheat cultivars will be helpful for gene deployment to control leaf rust.

A three-year field experiment was carried out to investigate the methane (CH₄) and nitrous oxide (N₂O) emissions and calculate the global warming potential (GWP) according to all energy input in response to the nitrogen (N) rate in the typical rice-wheat rotation system in Jiangsu, China. Four N treatments, including R220W180 (local practice), R220W140 (cutting 10% total N in wheat season), R180W180 (cutting 10% total N in rice season) and R180W140 (cutting 20% total N in rice and wheat seasons separately), were designed in the study. Results showed that annual CH₄ emission was decreased by 25.7% in response to cutting 20% N, which was ascribed to the 24.6% reduction of CH₄ emission in rice season (P < 0.05) compared to local practice. The mitigation of N₂O emissions in R220W140 and R180R180 treatments contributed to the 8.5% and 15.7% decrease in annual N₂O emission, which was the 23.5% decrease in cutting 20% N treatment compared to local practice, respectively. Specifically, under the same amount of N rate condition (10% N cutting), the transfer N from rice season (R220W140) to wheat season (R180W180) led to the 8.5% increase in N₂O emission (P < 0.05). In the end, the cutting of 20% N decreased GWP and yield-scale GWP by 19% and 17%, which mainly originated from CH₄ and N₂O emissions. However, cutting N did not significantly decrease grain yield (P > 0.05). These results suggested that the 180 kg N/ha for rice and 140 kg N/ha for wheat in one rotation season were the beneficial N rate to achieve the co-benefit of yield and GWP in the typical rice-wheat rotation system in Jiangsu, China.

Drought is an important agricultural problem worldwide, which seriously affects the growth and yield of crops. To explore the effects of different degrees of drought on the soluble sugar content of soybeans, this study measured the soluble sugar content of two soybean cultivars at three growth stages under four levels of drought stress. The results showed that drought stress significantly affected the soluble sugar content, and there were differences among different growth stages and cultivars. At the seedling and flowering stages, the sucrose content of both Heinong44 and Heinong65 showed an unimodal trend and reached the maximum value at moderate drought. The increase rate was the highest in the leaves at the flowering stage, which increased by 36.18% and 25.79% compared with CK, respectively. The fructose and glucose contents were the highest during severe drought, and the fructose content increased the most in the leaves at the seedling stage, which increased by 18.05% and 17.67% compared with CK, respectively. The glucose content increased the most in the petioles at the flowering stage, reaching 40.66% and 35.24%. At the pod-filling stage, the three sugar contents of both Heinong44 and Heinong65 were the lowest at severe drought, and the sucrose and fructose contents decreased the most in the petioles, which decreased by 21.66% and 23.94%, 12.58% and 13.49% compared with CK, respectively. The glucose content decreased the most in the stems, which decreased by 11.72% and 9.66%. In addition, at each growth stage and drought treatment, the ratio of the soluble sugar content of Heinong44 was higher than that of Heinong65.

The Seriphidium transiliense desert pasture is an important spring-autumn pasture in northern Xinjiang, China, and has been subjected to grazing by livestock at different intensities, thus resulting in widespread deterioration of its biodiversity and ecosystem services. To understand the response mechanism of stoichiometric characteristics of desert vegetation to grazing, the leaf carbon (C), nitrogen (N), phosphorus (P) and C : N : P ratios of S. transiliense were studied under different grazing intensities. The results show that the control S. transiliense leaf C, N and P contents and C : N, C : P and N : P ratios were 458.79 ± 53.5 g/kg, 20.6 ± 7.18 g/kg, 2.83 ± 1.24 g/kg, 25.69 ± 11.08, 190.28 ± 75.65 and 8.21 ± 4.01, respectively. The differences in these characteristics varied with grazing intensity in accordance with sampling time, so both factors need to be considered comprehensively. General linear model (GLM) analysis indicated that grazing intensity had a strong main effect on S. transiliense leaf C, N, and P content, C : N ratio and N : P ratio. As grazing intensity increased, the leaf N content and N : P ratio increased (P < 0.01), and the C : N ratio decreased (P < 0.01). N content was the limiting factor for the growth of S. transiliense, but the grazing intensity, sampling year and growth season each affected the degree of N limitation. Our findings suggest that the remaining moderate stocking rate was essential for sustaining desert stabilisation in Xinjiang, and although S. transiliense could adapt its nutrient content and leaf stoichiometry to the grazing intensity, N was always the limiting element for the growth of S. transiliense.

For sustainable breeding in potato, a better understanding of genetic diversity within germplasm banks for sustainable breeding is needed. This study comprehensively characterised the molecular and phenotypic traits of 62 potato accessions, including advanced clones and indigenous potato varieties from Advanced Chemical Industries Limited (ACI Ltd.), Bangladesh, and 8 varieties from the Bangladesh Agricultural Research Institute (BARI). By using 9 SSR markers and 13 morphological traits, including both quantitative and qualitative traits, we observed correlation coefficients ranging from –0.3 to 0.7 for 8 quantitative traits, and Pearson's chi-square (χ² value) ranging from 24.3 to 135.4 for 5 qualitative characteristics. Molecular analyses identified 46 unique alleles, with 93.5% polymorphism. The markers STM0031 and STM1016 had the highest PIC value of 0.9. Genetic parameters for SSR markers included effective number of alleles per locus (Ne) = 5.6, unbiased expected heterozygosity (uh) = 0.8, diversity (h) = 0.8 and Shannon’s information index (I) = 1.8. Jaccard’s similarity coefficients ranged from 0.2 to 0.8, representing significant diversity. Cluster analysis, using unweighted pair-group method with arithmetic average (UPGMA), grouped the accessions into five clusters based on SSR profiles. An association was foud between the marker STM0031 and two traits: the number of tubers per hill and the content of reducing sugars in the tubers. This study provides information on genetic diversity and marker efficacy. It will guide future breeding programmes towards the development of high-yielding and industrially valuable potato varieties.

This study investigates the extent to which international agricultural price shocks are transmitted to domestic agricultural markets in both developed and developing economies. Using panel data from 13 major agricultural trading countries from 1990 to 2022, we applied cross-sectional dependence tests, fixed-effects panel regressions, and panel Granger causality analyses to examine the degree of interdependence and directionality in price movements. Our results reveal significant cross-sectional dependence, suggesting that global price shocks reverberate across the national markets. The panel regression estimates indicate a statistically significant and positive effect of international prices on domestic prices, with a stronger transmission observed in developed economies. Panel Granger causality tests confirm that global price indices significantly predict domestic price changes in most countries, although reverse causality is limited to large exporters, such as the United States, China, and Brazil. Robustness checks across sub-periods (1990–2005 vs. 2006–2022) and country groupings validate the stability and heterogeneity of the price transmission mechanisms. These findings underscore the increasingly integrated nature of food markets and the evolving dynamics of price volatility. The policy implications highlight the importance of multilateral coordination in food trade, differentiated policy tools tailored to country development levels, and the need for strategic buffering mechanisms to manage the growing global volatility in agricultural prices.

This study compares soil temperature data collected between 2019 and 2022 in Hrušky, South Moravia, Czech Republic. Soil temperature was measured at five depths (5, 10, 20, 50, 100 cm) in the forest shelterbelt (windbreak) and at three distances from it to investigate the impact of the shelterbelt on the climatic conditions of adjacent field plots. In particular, monthly averages, calculated from average daily temperatures, were employed to characterise the temperature course. These are calculated as averages of measured temperatures at 15-minute intervals. Absolute and relative differences and, where appropriate, base indices, were calculated to facilitate the comparison of individual measurement points (sites) and soil depths. The soil temperature values and their dynamics during the year differ between the measurement point in the forest shelterbelt (90-0) and those in the field. Additionally, the field measurement points exhibit some degree of variation, with the more distant field measurement point (180-90) displaying distinct characteristics from the closer field measurement points (90-45, 90-90). During the winter months (December, January, February), the temperature increases with soil depth, being highest within the windbreak. In spring (February and March), the temperature at different soil depths starts to equalise; however, in April, the temperature decreases with soil depth. Throughout the summer, the measurement station within the windbreak has lower temperatures than in the field, where the soil shows higher temperatures at all depths compared to the windbreak measurement station. In August, the temperature differences in depth begin to equalise again. In September, the temperature trend reverses, and from October, the temperatures increase with soil depth, especially in the lower layers of the soil. The temperature trend in November has a more or less winter character. Soil temperatures in the forest shelterbelt are lower in the summer months and higher in the winter months than in the field. The protective effect of the windbreak is more pronounced at measurement stations closer to the belt, as the temperatures at the farthest field measurement station are higher in summer and lower in winter compared to the closer field measurement stations.

The grain filling and physiological traits of different phosphorus-tolerant rice cultivars and phosphorus fertiliser rates have not been fully studied. A pot-growth experiment with cv. Lianjing 7 (weak phosphorus tolerance) and cv. Yongyou 2640 (strong phosphorus tolerance) was conducted using four phosphorus rates, namely, 0 (P0), 0.44 (P1), 0.88 (P2), and 1.32 g/pot (P3). Results indicated that grain yield, net photosynthetic rate, soil and plant analyser development (SPAD) value, superoxide dismutase (SOD) and catalase (CAT) activity in leaves, and adenosine diphosphate glucose pyrophosphorylase (AGPase) and sucrose synthase (SuSase) activity in grains increased and then decreased with increasing phosphorus fertiliser rate, whereas malondialdehyde (MDA) content in leaves decreased first and then increased. The above indexes of cv. Lianjing 7 and cv. Yongyou 2640 were optimal at P2 and P1 treatments, respectively. The grain yield, net photosynthetic rate, SPAD value, AGPase content, SuSase content in grains, and SOD and CAT activity in the leaves of cv. Yongyou 2640 were higher, whereas the MDA content was lower than those of cv. Lianjing 7. Correlation analysis showed that AGPase and SuSase activity in superior and inferior grains, photosynthetic rate, and SOD and CAT activity in the leaves were significant or highly significantly positively correlated with grain-filling rate and rice yield. Therefore, the adoption of appropriate phosphorus fertiliser rates can increase the activity of enzymes related to starch synthesis in different phosphorus-tolerant rice, enhance antioxidant systems in leaves at the filling stage, reduce leaf MDA content, and delay leaf senescence. These effects are beneficial to grain filling and increase grain yield.

The Asian rice gall midge (Orseolia oryzae) (Wood-Mason) is a major pest of rice, significantly reducing yields and challenging sustainable rice production. This review provides a comprehensive overview of the biology, lifecycle and geographical distribution of the rice gall midge, along with the damage symptoms it causes in rice crops. The interactions between rice and gall midge, the pest's infestation mechanism and the plant’s defensive responses are also explored. Various management strategies are discussed in detail, including insecticides, cultural practices and resistant varieties. The review emphasises that breeding for resistance, especially through the pyramiding of resistance genes and integrated pest management approaches, shows the most promise for long-term control. Advances in crop improvement through breeding methods such as genotyping, phenotyping, field and greenhouse screening and the pyramiding of resistance genes are highlighted. The review emphasises the importance of monitoring virulence in gall midge populations to guide breeding efforts. The genetic basis of resistance is examined through studies of resistance genes, QTL mapping and marker-assisted breeding. Furthermore, molecular approaches, including metabolomic regulations, microarray analysis and biotechnological strategies, are reviewed for their potential in developing durable gall midge-resistant rice varieties. This article synthesises the current knowledge and highlights future research directions, such as identifying novel resistance genes, improving molecular breeding techniques, and developing integrated pest management strategies that combine genetic resistance with eco-friendly controls.

Environmental conditions are known to influence the dairy cow health, with most research focusing on the detrimental effects of heat stress. However, the impact of non-summer weather patterns in temperate climates on udder health carry-over between lactations is not well understood. This pilot study evaluated the effect of various weather factors (temperature, humidity, precipitation, sunshine) during 1-day to 90-day periods before dry-off on udder health in the first month post-calving (represented by somatic cell count, conductivity, lactose, and mastitis incidence) in 199 Holstein cows in Central Europe. The scope of the experiment was limited to one farm and one year of observations. Moreover, we also evaluated milk quality, milkability, and udder health parameters during the same periods before dry-off on udder health after calving. We aimed to identify viable indicators across available automatically collected data from weather stations and milking parlour analysers. We found that the long-term (30- to 90-day) exposure to colder temperatures, high humidity, and low sunshine duration before dry-off was significantly associated with worse udder health after calving. In contrast, short-term weather conditions (1- to 7-day) had no significant effect. Monitored milk quality and udder health parameters showed a significant relation to udder health after calving during the immediate periods before dry-off, while milkability parameters were insignificant. If lactose was decreased or protein content, conductivity, and somatic cell count were elevated during the 1- and 7-day period before dry-off, cows after calving showed worse udder health. These findings identify prolonged cold and damp conditions as a significant environmental risk factor for poor udder health in the subsequent lactation, expanding our understanding beyond the conventional focus on heat stress.

There is significant interest in the propagation of bananas due to their substantial global economic importance, which is why this study aimed to develop an efficient protocol for in vitro propagation through somatic embryogenesis (SE) of the ‘Manzano’ and ‘Pelipita’ cultivars. Immature male flower bud explants were used for embryogenic callus induction. The ‘Manzano’ cultivar showed a higher percentage of embryogenic callus induction than ‘Pelipita’. Stereomicroscopic observations revealed that the embryogenic callus was asynchronous, as various stages of somatic embryos were noted. White translucent pro-embryogenic callus was efficiently utilized to establish embryogenic cell suspension. The development of different stages in the regeneration process of embryogenic cell suspension was also recorded. Embryos in the late coleoptilar stage, characterized by an opaque white morphology, were selected and transferred to an appropriate medium to evaluate the germination percentage. We found that the germination rate was highly efficient for both cultivars, exceeding 90% when using these embryos in the selected stage. Additionally, we observed that embryos that were 90 days old showed a 10–12% increase in germination compared to those that were 60 days old, confirming the importance of embryo maturation duration and the selection of embryogenic aggregates through the sieving of suspended cells to optimize regeneration efficiency.

Recent advancements in remote sensing techniques, especially the combination of hyperspectral imaging with analytical algorithms, have greatly improved precision agriculture. This study introduces some algorithms developed for identifying crops and evaluating their growth conditions, focusing on irrigation and fertilisation. The present approach is based on the concept of identifiability of a family of dynamic systems and the differentiation of plants using their spectral signatures. The method uses a repository of spectral data and applies a developed algorithm to compare the measured spectra with the reference database, enabling the identifiability and the recognition of both known and unknown crops. As an application of our approach, we have considered two different crops: mint and rosemary, under different irrigation and fertilisation conditions. The results show that the algorithm achieved a 100% identification rate across the four unknown samples. The minimum spectral distances obtained are 0.01 and 0.03 for rosemary and mint, respectively. Thus, the family of systems was identifiable with a tolerance of η < 0.03. The study concluded that the algorithm effectively classifies the crop type and deduces its growth conditions, demonstrating its effectiveness for agricultural monitoring.

This study evaluated the effects of hybrid, vegetation period duration, weather conditions, and harvest timing on sugar beet (Beta vulgaris L.) yield and technological quality under short-rotation cropping systems in the Western Forest-Steppe of Ukraine. Field experiments were conducted in 2022–2024 on commercial fields using six industrial hybrids and five harvest intervals from late September to mid-November. Root yield, sugar content, sugar yield, α-amino nitrogen, K⁺ and Na⁺, invert sugars, and the technological quality index (Iq) were assessed using ANOVA, correlation analysis, and principal component analysis (PCA). Extending vegetation from 185 to 200 days increased root yield by 11–12% and sugar yield by 0.8–1.2 t/ha. The optimal harvest window (10–25 October) provided the highest performance, with root yields of 68–73 t/ha, sugar content of 16.2–16.6%, and sugar yields of 14.6–16.3 t/ha. Early harvest resulted in reduced sugar content and Iq, whereas harvesting after 10 November did not increase yield and caused deterioration of technological quality due to elevated α-amino nitrogen and molasses-forming ions. PCA showed that over 85% of the total variation was explained by technological quality and moisture-related factors. Strube hybrids demonstrated greater stability under extended vegetation compared with KWS hybrids. These results define an optimal harvest window for maximising sugar beet productivity and quality under temperate meteorological conditions.

Despite the recognised benefits of climate smart agriculture (CSA) in enhancing farmers' adaptive capacity to climate risks, adoption rates remain low in Sub-Saharan Africa. This disparity can be attributed, in part, to the significant challenges smallholder farmers face in accessing credit from the formal financial sector. In response, Rural Saving and Credit Cooperatives (RUSACCOs) have emerged as crucial sources of funding for both household expenses and agricultural activities. However, despite their increasing importance in improving financial inclusion, little is known about whether participation in RUSACCOs can help alleviate existing credit constraints and promote the adoption of CSA among smallholder farmers. To address this knowledge gap, we employ a recursive bivariate probit (RBP) and propensity score matching (PSM) analysis using data from 400 randomly selected smallholder farmers in Zambia. The analysis controls for three main sources of endogeneity: program placement, endogenous covariates, and self-selection. Our findings indicate that participation in RUSACCOs has the potential to mitigate farmers' credit constraints by 42% and facilitate CSA adoption by 25%. Notably, the alleviation of existing credit constraints is associated with a 14% increase in CSA adoption. These results underscore the previously overlooked role of RUSACCOs in promoting agricultural sustainability. By effectively addressing financial inclusion barriers and providing access to practical agricultural knowledge, RUSACCOs can contribute to reducing the vulnerability of agriculture while fostering sustainable production. Our study suggests that repurposing RUSACCOs to emphasise financial inclusion and promote access to agricultural learning platforms can yield triple benefits: agricultural, environmental, and livelihood sustainability.

The rhizosphere plays an important role in both farmland and urban areas, affecting water quantity and quality during surface water infiltration by increasing the heterogeneity of the aeration zone. The extensive application of antibiotics, their recalcitrance to degradation, and the resultant accumulation of antibiotics in soil-microbe-plant systems represent significant threats to the rhizosphere system, thereby threatening ecological stability and environmental and human health. This review synthesises recent findings on the migration and transformation of typical and common antibiotics within the rhizosphere. The main findings include that the absorption of antibiotics by plants is influenced by their molecular weight (MW) and octanol-water partition coefficient (log K_ow), allowing antibiotics to be divided into three classes: (1) antibiotics with high lipophilicity (log K_ow > 2) are mostly adsorbed by root lipids and rarely participate in the soil-plant transport process; (2) antibiotics with log K_ow < 2 and high MWs (MW > 700) are blocked outside the plant roots; and (3) antibiotics with log K_ow < 2 and low MWs (MW < 700) can enter plants through the roots and are transported via transpiration flow in plants. Antibiotics with log K_ow < 1 are more easily transported into plant tissues, including leaves. The rhizospheric microorganisms capable of participating in antibiotic migration and transformation are concentrated in Actinobacteria, Firmicutes, Proteobacteria, and Bacteroidetes. The inhibitory effect of antibiotics on dehydrogenase, sucrase, urease, catalase, and alkaline phosphatase activities surpasses their promoting effect, reducing these enzyme activities by 6–35% on average. However, the promoting effect of antibiotics on peroxidase, acidic phosphatase, and manganese peroxidase outweighs the inhibitory effect, increasing enzyme activity by 2–23%. Furthermore, it is essential to consider the effects of plant age and root characteristics on antibiotic migration and transformation. The results of this review contribute to a better understanding of the migration and transformation of antibiotics within the rhizosphere.

Modern dairy farming faces the dual challenge of meeting global food demands while mitigating its environmental impact, particularly greenhouse gas (GHG) emissions, such as methane (CH₄), a potent contributor to climate change. This review explores the role of Precision Livestock Farming (PLF) technologies in monitoring and reducing CH₄ emissions from dairy cattle. We evaluate state-of-the-art methods, including direct monitoring (e.g. respiratory chambers, GreenFeed systems) and indirect approaches (e.g. infrared milk spectroscopy, AI-driven analytics), alongside mitigation strategies such as nutritional optimisation, genetic selection, and ruminal additives. PLF emerges as a transformative tool, integrating real-time data on animal health, feed efficiency, and environmental conditions to optimise management practices and reduce emissions per unit of milk produced. By synthesising current research, we highlight the potential of PLF to reconcile productivity with sustainability, offering scalable solutions for the dairy sector. Critical gaps in real-time CH₄ monitoring and farm-level implementation are identified, underscoring the need for further innovation. This review provides a roadmap for aligning dairy production with global climate goals while ensuring food security for the growing population.

The intensification of agricultural practices in Bangladesh has caused significant environmental challenges. This has also undermined farmers' economic sustainability, mainly due to the excessive use of subsidised chemical fertilisers. To address these issues and align with the United Nations Sustainable Development Goals (SDGs), Bangladesh has prioritised the adoption of sustainable farming practices, including the recommended fertiliser application (RFA). However, whether the adoption of RFA ensures economic sustainability remains uncertain. This study evaluates how the Bangladesh Rice Research Institute's (BRRI) proposed RFA affects fertiliser use and cost-efficiency. Drawing on five years (2017–2021) of panel data from 2 025 households across three acidic soil regions in Dinajpur, the findings reveal that RFA adoption reduces fertiliser use by 12% while improving cost efficiency by 4.9–5.1%. These results highlight the potential of RFA to mitigate environmental degradation while enhancing economic outcomes, thereby supporting the SDG agenda. In light of these benefits, the study offers key insights for policymakers and development practitioners, emphasising the need for targeted interventions to accelerate RFA adoption and promote sustainable agriculture.

Premature ovarian failure (POF) is a significant cause of infertility and is often linked to autoimmune aetiologies. Lipopolysaccharide (LPS)-induced inflammation is a well-established model of autoimmune POF in rodents. Immunomodulatory treatments involving corticosteroids, frankincense, and targeted secondary antibodies have been hypothesised to mitigate the autoimmune response, reduce anti-ovarian antibody (AOA) levels, and restore ovarian function in an LPS-induced POF rat model. A POF model was established in female albino rats via the intraperitoneal injection of LPS. The rats were then divided into groups that received no treatment (LPS control), dexamethasone (DEX-treated LPS-treated rats), methylprednisolone (MP-treated LPS-treated rats), frankincense (Frankincense-treated LPS-treated rats), or secondary anti-ovarian antibodies (secondary Ab-treated LPS-treated rats) for 3 to 4 weeks. The serum levels of AOA, 17β-oestradiol, follicle-stimulating hormone (FSH), and luteinising hormone (LH) were assayed via commercial enzyme-linked immunosorbent assay (ELISA) kits. Ovarian tissues were examined histopathologically to assess structural damage and recovery. LPS induction successfully created a POF phenotype, as evidenced by significantly elevated AOA levels (P < 0.001), reduced 17β-oestradiol (P < 0.001), elevated FSH/LH (P < 0.001 and P < 0.05, respectively), and severe histopathological damage, including follicular atresia. All the treatments restored 17β-oestradiol levels. Secondary antibody therapy was most effective, normalising all hormonal parameters, significantly reducing AOA levels, and demonstrating complete histological recovery with healthy follicles and corpora lutea. MP potently suppressed AOA but paradoxically elevated FSH, without improving ovarian histology. DEX and frankincense showed intermediate efficacy, improving some hormonal and serological markers but failing to achieve full histological restoration. These findings demonstrate that targeted immunotherapy using secondary antibodies is superior to broad immunosuppression or anti-inflammatory treatment for restoring ovarian function in patients with autoimmune POF. While corticosteroids effectively reduce AOA titres, they may not reverse ovarian damage and can disrupt the hormonal balance. This underscores the need for precise, biomarker-guided therapies over nonspecific immunosuppression in patients with autoimmune ovarian insufficiency.

Mastitis is a complex, common and economically problematic issue in dairy cattle production. In this study, breast cancer 1 (BRCA1) and toll-like receptor 1 (TLR1) genes were taken as candidate genes for mastitis resistance. This study investigated whether BRCA1 and TLR1 genes were associated with milk production traits (daily milk yield, fat, and protein content) and somatic cell score (SCS). A total of 105 cows (25 Holstein, 48 Simmental, and 32 crossbred Holstein) were genotyped using the PCR-RFLP method. Cows with the BRCA1 c.46126G>T GG genotype had significantly lower SCS than the other genotypes. The TLR1 g.60438363C>T SNP influenced the protein content in all cows, with genotype CC having a higher content than TC. The same SNP in the Simmental breed showed that cows with the CC genotype had significantly higher SCS than the heterozygote. Cows with the GG genotype of TLR1 g.60437324A>G had significantly lower SCS and higher fat and protein content than the heterozygote. The results of this study indicate that BRCA1 c.46126G>T and TLR1 g.60437324A>G SNPs could be useful for improving mastitis resistance in dairy cattle through marker-assisted selection.