Fulltext search in archive

However, its underlying mechanism remains largely undetermined. In this work, we examined the impacts of the continuous application of pig manure fertiliser for 10 years on wheat yield in the calcaric fluvisol soil with a texture of sandy loam, and the relationship between soil microbial community composition and soil properties was also analysed. The wheat yield, yield components and wheat biomass were analysed by collected aboveground part. Soil nutrient, enzymatic activity and microorganism compositions were analysed by collected soil samples at the filling stage. The results showed that long-term application of pig manure fertiliser could remarkably increase wheat yield by improving soil nutrient availability, enzyme activities, and microbial composition. Moreover, soil pH, nitrogen content, dehydrogenase and urease were closely related to the soil microbial diversity. In conclusions, the long-term application of pig manure in combination with term mineral fertiliser could optimise microbial community composition by regulating the interaction between microbial species and enhancing soil enzyme activity and soil fertility, leading to increased wheat yield.

Sap flow represents water transport from roots to leaves through the xylem and is used to describe tree transpiration. This paper proposed and tested a procedure to estimate sap flow by calculating global radiation in a digital model of the tree canopy surface obtained by unmanned aerial vehicle imaging. The sap flow of nine trees was continuously measured in the field. In the digital surface model, individual canopies were automatically delineated, their parameters were determined and the global radiation incident on their surface on specific days was calculated. A polynomial relationship was found between sap flow and the calculated incident solar radiation during the morning hours with a coefficient of determination of 0.98, as well as a linear relationship between the decrease in radiation and sap flow during the afternoon with a correlation coefficient of 0.99. Using the Random Forest machine learning method, a model predicting the sap flow of the trees was created based on the global radiation and canopy parameters determined from the digital surface model of tree canopies. The resulting model was deployed on additional days and compared to field measurements of sap flow, achieving a correlation coefficient of 0.918. In addition, two linear regression models were created for a tree group, achieving coefficients of determination of 0.66 and 0.90.

This study aimed to evaluate the effects of whole-plant corn silage (WCS) on growth performance, nutrient availability and intestinal development in growing-finishing pigs. A total of 32 barrows (33.1 ± 3.49 kg) were randomly allocated to four treatments. Control was the basal diet, and the low, medium, and high treatments were basal diets substituted with WCS (air-dry basis) at 5.0%, 7.5%, and 10.0% from day 1 to 42, and 10.0%, 12.5%, and 15.0% from day 43 to 98, respectively. The results showed that the average daily feed intake (ADFI), feed/gain (F/G), and crypt depth (CD) increased linearly (P < 0.05) with the increasing of WCS. However, the final body weight, average daily gain (ADG), biological value (BV), net protein utilization (NPU), dry matter, crude protein (CP), ether extract, crude fibre (CF), and gross energy decreased linearly (P < 0.05). High treatment significantly deteriorated BW, ADG, NPU, and BV compared to control, low, and medium treatments (P < 0.05). The low and medium treatments showed significantly greater ADFI, apparent digestibility (CP and CF), villus height (VH), VH/CD, relative mRNA and protein expression of occludin, claudin-1 and sodium-glucose cotransporter (SGLT1) than the high treatment (P < 0.05). The highest F/G, CD, relative mRNA and protein expression of cationic amino acid transporter (CAT1) were observed in high treatment (P < 0.05). Our results suggested that WCS can replace 7.5% and 12.5% of diet during growing and finishing periods of pigs, respectively. This is of great significance for reducing feeding costs and alleviating food security crises.

The food industry is always looking for ways to innovate in elaborating and packaging food products to ensure the consumer receives the highest quality. The new proposals include the use of polycaprolactone (PCL), a commonly used biopolymer that is soluble in many organic solvents. The PCL features can be modified by forming blends with other polymers and bioactive molecules to expand their applications in the food industry. For instance, there have been developments in packages and active substances that incorporate microcapsules based on PCL. This review explores the applications of PCL in the food industry, encompassing its role as a biodegradable active package and as an encapsulating agent. The review underscores the potential of this polymer in the context of the food industry.

The land in the South Tasikmalaya Regency is dominated by dry land with a steep and hilly land contour. In addition, the area has a shallow soil solum, so it has a high potential for erosion and landslides. Therefore, it is necessary to innovate an integrated cultivation system that is economically feasible, but still maintains environmental sustainability. In this research, the aim is to formulate a model for the development of integrated plantation polyculture farming (IPPF) for the welfare of farmers. The method in this study uses a mixed method design. The location of the research carried out is in the Tasikmalaya Regency. The data used are the results of interviews and questionnaires to 250 IPPF farmers. The data used were obtained by multistage cluster random sampling. The sustainable IPPF development model was analysed using Structural Equation Modelling (SEM). This analysis is used to determine the various potential capital factors for agricultural development on the sustainable development of the IPPF and farmers’ welfare. The results of the study show that the potential development capital [natural resources (SDA) capital, economic capital, socio-cultural capital, physical capital, and multifunctional IPPF], jointly or partially affect the IPPF sustainability. Human resource capital has no effect on the sustainable IPPF development, while the sustainable IPPF development affects the farmers’ welfare levels, and the potential agricultural development capital and its multifunctionality affect the sustainable IPPF development and farmers’ welfare. The IPPF development model that improves the welfare of farmers is carried out through the development of specific local superior commodities, increasing the added value and cooperation, developing access to credit, regenerating farmers, sustainable assistance and infrastructure improvements. The results of this study are expected to be a reference for the government in improving IPPF sustainability to achieve the welfare of its farmers. Apart from that, the model that has been developed can make a contribution to science in the form of a comprehensive analysis of the factors that influence the sustainability of IPPF.

The aim of the study was to assess the impact of three biostimulants and a herbicide on selected unit parameters of potato yield. The research results came from a three-year field experiment that was established using the randomised sub-blocks method in three replications. In the experiment, two cultivars of edible potato were grown and the yield-protective effect of treatments was assessed: (1) control object without protection with preparations; (2) herbicide clomazone + metribuzin; (3) biostimulant PlonoStart and herbicide clomazone + metribuzin; (4) biostimulant Aminoplant and herbicide clomazone + metribuzin, and (5) biostimulant Agro-Sorb Folium and herbicide clomazone + metribuzin. As a result of the limited competition of weeds in the objects with the use of biostimulant and herbicide, a higher average weight of one potato tuber, a higher share of commercial tubers > 35 mm and a higher share of large tubers > 50 mm was obtained compared to the control object. The highest average weight of one tuber (108.2 and 85.4 g, respectively) and the highest share of commercial (97.1 and 96.3%, respectively) and large tubers (60.6 and 60.5%, respectively) were obtained in the objects where the Agro-Sorb Folium biostimulant + Avatar 293 ZC herbicide were used. and PlonoStart + Avatar 293 ZC. The correlation analysis confirmed a significant negative relationship between the number of weeds and average weight of one potato tuber, share of commercial tubers and share of large tubers. Weather conditions during the conduct of the research also significantly differentiated weed numbers and quality parameters of potato yield.

The impact of toxic elements (TEs) contaminating the root zone of Lupinus angustifolius L. on enzymatic activities, nitrification rate, and changes in the root system was evaluated. Lupine was cultivated in a pot experiment using two types of soil – control and contamination (with a high degree of arsenic (As), cadmium (Cd), lead (Pb), and zinc (Zn) contamination). After harvesting lupine biomass, enzyme activities (β-glucosidase, acid phosphatase, arylsulphatase, lipase, chitinase, cellobiohydrolase, alanine aminopeptidase, and leucine aminopeptidase) in soils were analysed. Enzyme activities decreased with TE soil contamination. According to our results, arylsulphatase was found to be the most sensitive soil enzyme to TEs. The nitrification rate is closely related to soil contamination and plant activity, as it stimulates microbial growth and multiplication through root exudates. The close correlations confirmed this relationship (r = 0.73−0.99). An increasing trend in TE contents in the roots was observed with soil contamination. Plant hormones are crucial in regulating root growth and development under stress conditions. The levels of determined phytohormones in our experiment (auxins, abscisic acid (ABA), salicylic acid (SA), and bioactive cytokinins (bCKs)) were lower in the contamination compared to the control. Correlations confirmed a significant negative relationship between the TE content in the roots and the contents of phytohormones (auxins: r = –0.96 to –0.97; ABA: r = –0.83 to –0.86; SA: r = –0.95 to –0.99, bCKs: r = –0.87 to –0.93). The ratios of these hormones (not their absolute values) appear to be the determining factor for regulating root development and protecting plants from oxidative stress.

The aim of this study was to determine the reference values of cartilage damage biomarkers in the blood and urine in 76 clinically healthy German Shepherd dogs. We grouped the dogs into 4 groups by age. All the groups were consistently made up of 19 dogs. The mean age and average body weight were established for all the dog groups. The blood was collected from the vena cephalica antebrachii or the vena saphena medialis and the urine sample was taken by us-guided cystocenthesis. The biomarkers were determined using quantitative sandwich ELISA kits. The mean values of the biomarkers ± SD were, for the urine biomarkers, CTX-I 3.29 ± 1.16 ng/ml, CTX-II 1 993.95 ± 777.04 ng/ml, and TIMP-I 392.80 ± 160.56 ng/ml, and for the blood biomarkers, MMP-9 89.85 ± 50.21 ng/ml and PIICP 19.19 ± 7.33 ng/ml. Based on the obtained values of the mean 95%, we expect a standard for CTX-I 5.05 ng/ml, CTX-II 3 204.26 ng/ml, TIMP-I 606.64 ng/ml, MMP-9 187.93 ng/ml, and PIICP 31.71 ng/ml.

Farmers, often vulnerable within the agricultural supply chain, frequently encounter difficulties accessing and affording loans. This study introduces an innovative credit risk evaluation framework for farmers tailored to the agricultural supply chain. It includes three key aspects: farmers’ credit characteristics, the operational status of the agricultural supply chain, and overall credit conditions. Initially, the analytic hierarchy process (AHP) was used to assign weight coefficients to indicators. Then, the Elimination et Choix Traduisant la Réalité III (ELECTRE III) model was employed to determine farmers’ credit ratings. To demonstrate the impact of the agricultural supply chain on microfinance, the model’s effectiveness was then tested with 398 microfinance survey responses from Fuping County (World Dairy Goat Industry Development Demonstration Zone), Shaanxi Province, China, and its accuracy was further verified using BP neural network analysis. The results demonstrated the model’s proficiency in assessing farmers’ credit levels within the agricultural supply chain, which can aid in the resolution of various credit assessment and rating challenges. Furthermore, this study offers valuable insights into the integration of multi-criteria decision-making and machine-learning methods.

Agriculture is one of the main contributors to carbon emissions and is also significantly affected by changing climate. This article investigates the impacts of agricultural support on greenhouse gas emissions (GHG) from agriculture in the European Union. Panel regression models were used to examine the influence of subsidies in the Common Agricultural Policy (CAP) on reducing GHGs from agriculture. First, estimates show that direct agricultural subsidies encouraged agricultural-related carbon emissions. Second, the study concludes that the expansion of organic agriculture and the rural development expenditures contributed to emissions reduction in the member states. Finally, the CAP Health Check reform decreased while the Ciolos reform stimulated GHG emissions.

The aim was to study the evolution and severity of white striping and wooden breast myopathies and to carry out a study on the parameters of meat quality and allometric growth of male and female broilers at different ages. The experiment was conducted in a broiler house in a completely randomized design, using 960 one-day-old chicks of the Cobb^® strain, with 480 chicks of each sex. 96 birds per treatment were slaughtered weekly at the age of 21 to 49 days. The present study analyses the occurrence of myopathies, their scores, and their evolution over the age of the birds, comparing males and females. Further assessments included the allometric coefficient and meat quality analyses such as physical measurements (breast length, width, and thickness), pH, colour (L*, a*, and b*), drip loss, water-holding capacity, cooking loss, and shear force. The results demonstrate that the sex of birds did not affect the occurrence of white striping and wooden breast myopathies. However, the degree of myopathies evolved with advancing age, suggesting the weight as a predisposing factor for these myopathies. The emergence of white striping myopathy occurred as early as in the third week of the birds’ life. Wooden breast did not develop as isolated myopathy, occurring only in the concomitant presence of white striping. The allometric coefficient demonstrated that regardless of the sex and age, broiler breast had positive heterogonic growth, i.e. late growth in relation to the other parts of the body.

Breadfruit's substantial carbohydrate content makes it a viable starch source, specifically resistant starch (RS) that helps prevent chronic diseases. This study investigated the effects of enzyme hydrolysis and microwave treatment (MT) on enriching type III RS in breadfruit. It also determined its structural and functional properties, including swelling power, solubility index, water absorption capacity, oil binding capacity, and syneresis. MT at 30 W·g^–1 for 3 min resulted in the highest RS content of 74.8%, significantly surpassing pullulanase hydrolysis (1.0 U·g^–1 dry basis for 12 h) at 17.3% RS. The breadfruit starch granules exhibited a regular shape, approximately 7.9 µm in length, whereas modified granules were less than 11 µm, along with observable deformation in their structural shape. In conclusion, the study demonstrates the efficacy of MT for enhancing RS content in breadfruit, highlighting its potential as a healthy functional ingredient and starch substitute.

Rosa damascena is one of the oldest valuable rose flower varieties that is almost drough tolerant. However, selecting and identifying landraces that are more tolerant to drought conditions will be effective in developing the cultivation of the plant under stress. The most important step in developing drought-tolerant plants is the evaluation and identification of resistant and susceptible genotypes. In this case, an experiment was performed under in vitro conditions using five levels (0, 25, 50, 75, and 100 g/L) of polyethylene glycol (PEG) on four landraces of the Damask rose (Maragheh, Urmia, Pakdasht, and Kashan). Based on the findings, the resistance of the different landraces to a water deficit was measured by the Design-Expert software using the response level (RSM) method. The results showed that Maragheh, with the highest fresh and dry weight, total chlorophyll, chlorophyll a, chlorophyll b, proline and an increase in the superoxide dismutase activity, had a high tolerance to drought stress. Moreover, Maragheh with a decreasing leaf number, height, malondialdehyde, hydrogen peroxide and having a higher membrane stability index, showed a better defence mechanism against oxidative stress than the other landraces. Also, after Maragheh, Pakdasht had the best performance compared to the other two landraces up to 75g/L of PEG, but not as well as Maragheh at the highest level of the tested PEG. Urmia and especially Kashan probably do not have much tolerance to drought stress regarding all the results and levels of desirability.

The black soldier fly (Hermetia illucens) serves as an alternative source of protein in poultry production. However, there is little available information on feeding live black soldier fly larvae (LBSFL) to hens. The present study filled this gap by testing the effect of dietary LBSFL on laying hen performance, feed economy, egg quality, and yolk fatty acid composition. In total, one hundred forty-four 25-week-old Charoen Pokphand Brown laying hens were assigned to four groups. Experimental birds were provided a basal diet supplemented with 0.0 (T1), 10.0 (T2), 20.0 (T3), or 30.0 (T4) g/kg LBSFL. Our results indicated that no effects on body weight gain, egg yield, egg weight, or egg mass (P > 0.05) were observed in any of the dietary LBSFL groups. The addition of 10.0 g/kg LBSFL decreased feed intake, feed conversion ratio, and feed cost (P < 0.05), contrary to two higher doses of LBSFL. Similarly, the egg quality parameters were not influenced (P > 0.05) by the dietary LBSFL except for the egg yolk colour which significantly decreased with increasing LBSFL levels. Furthermore, the values for lightness (L*), redness (a*), and yellowness (b*) of the egg yolk were different (P < 0.05) between the four treatments. Paler egg yolk was found in the 20.0 and 30.0 g/kg LBSFL groups compared to the control group (P < 0.05). Significantly lower deposition of C18:3n6 was found for 30.0 g/kg, whereas there was a significant decrease in deposition of C22:6n3 with all LBSFL groups (P < 0.05). C20:3n6 levels, on the other hand, increased significantly in the 20.0 g/kg LBSFL group (P < 0.05). Thus, feeding LBSFL has no potential to increase the proportion of anti-inflammatory n-3 fatty acids. From an economic point of view, it is advantageous to feed the hens a dose of 10 g/kg LBSFL.

The aim of this study is to present the seasonal variation of organic matter (OM), total organic carbon (TOC), total nitrogen (TN), and total phosphorus (TP) in the bottom sediments of Marchica Lagoon, a post-restorated lagoon located in the Moroccan Mediterranean, and to estimate the potential role of the geochemistry and granulometry of bottom sediments in the retention of old eutrophication. The organic index of bottom sediment was found generally higher in old-eutrophic and enclosed areas. The surface sediments show variable levels of OM, TOC, TP, and TN; high in some cases (12.3%, 6.71%, 0.70%, and 0.30% respectively). These high levels were recorded in the central part of the lagoon and in areas subjected to high anthropogenic pressure, including wastewater discharges. Our results indicated that sediments are an excellent immobilizer of organic matter (C, N, and P), although, there was no correlation between sediment grain size and organic matter content. The absence of correlation might be explained by currentology and hydrodynamics, but also by the bathymetry of the lagoon. Therefore, sediments must be implicated in any lagoon restoration strategy and the site development plan.

The present study aimed to use a non-parametric data envelopment analysis (DEA) to estimate the energy efficiency and greenhouse gas (GHG) emissions in off-season cucumber-producing greenhouses in different farm level management systems in Iran. Data were collected using a questionnaire completed by 83 cucumber producers through face-to-face interviews. The results showed that the energy use rate was 75.1%, 82.6%, and 86.2% in small (0.5–0.9 ha), medium (0.9–2 ha), and large farms (≥ 2 ha), respectively. In all the farm levels, the greatest energy use shares belonged to electricity, chemical fertilisers, and human labour, respectively. The results of the DEA revealed that the technical, pure technical, and scale efficiencies of the large farms were 87.3%, 92.8%, and 97.1%, respectively, which were higher compared to other farm level management systems. The ratio of energy savings was estimated at 5.62% and 2.97% for small and large farms, respectively. The results also showed that electricity, chemical fertilisers, and diesel fuel were the most responsible for the GHG emissions. By optimising the energy use, pollution per one/ha one of off-season cucumbers can be mitigated by 1 614.5 and 1 315.0 kg of CO2/ha in small and large greenhouses; indicating more attention is required in managing the energy inputs in small-sized agro-ecosystems, especially for electricity.

The aim of the study was to evaluate the effect of different live weights at slaughter (LWS) of Zwartbles lambs on their daily gain (DG), carcass traits (CT) and chemical and physical characteristics of the quadriceps femoris muscle (QFM). A total of four weight groups of lambs were evaluated: group A, LWS = up to 35 kg; group B, LWS from 35.1 to 40 kg; group C, LWS from 40.1 to 45 kg and group D, LWS from 45.1 to 50 kg. The LWS had a significant (P < 0.05) effect on DG and most CTs, when average DG and most weights of individual CTs increased with increasing LWS. The evaluation of the influence of LWS on the chemical and physical characteristics of QFM primarily shows that this factor had a significant (P < 0.05) effect only on the content of intramuscular fat (IMF) and redness index (RI), when in both cases the values of these traits increased (IMF: from 0.57 to 1.21%; RI: from 8.53 to 9.76) with increasing LWS. In conclusion, it can be stated that most of the monitored traits in all weight groups of Zwartbles lambs were comparable with their levels in specialized meat breeds of sheep.

We conducted a quantitative ex post analysis of the competitiveness of the Czech Republic’s agrifood exports to other European Union (EU) Member States between 2002 and 2020, using the constant market share (CMS) methodology. The application of this methodology to Czech agrifood exports is novel, as it allows for analysing the export competitiveness variation and its components. Although the findings are consistent with those of previous studies in which the investigators used alternative methodological approaches, we make three contributions to the existing literature. Firstly, the Czech Republic experienced an overall increase in its competitiveness in agrifood exports between 2002 and 2020. Secondly, the high territorial specialisation of Czech agrifood exports to other EU Member States, combined with a general slowdown in the growth of EU markets, has had a significant effect on the growth dynamics of the country’s agricultural exports. Thirdly, there is a problem with the specialisation profile of Czech agrifood exports in terms of commodity and territorial structure. The current composition does not allow the Czech Republic to reach its full potential for agrifood exports. These findings will help industrial, agricultural and trade policymakers to assess the nature of liberalisation and transformation of the Czech Republic’s agricultural sectors, with a view to improving or designing structures and instruments for the promotion of agrifood exports.

The physiological regulation of Welsh onion seedlings was explored under short- and long-term drought stress. The Welsh onion cultivar ‘Neicong 3’ was grown in pots under drought stress (22.5–25% soil water content) or controlled watering conditions (65–67.5% soil water content). Different lengths of drought stress (7, 14, 21, and 28 days) were studied to determine the effects of the drought on the levels of the osmoregulatory substances, including proline (Pro), free amino acids (FAAs), soluble sugar (SS), and soluble protein (SP); the membrane-damage indicator malondialdehyde (MDA); the endogenous hormones indoleacetic acid (IAA), gibberellic acid (GA), abscisic acid (ABA), and jasmonic acid (JA); and the activities of the antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD). The soil water content was restored to the control level after the drought stress to analyse the physiological responses of the Welsh onion with regards to rehydration. The results showed that the Welsh onion responded to drought stress primarily by increasing the levels of the osmoregulatory substances and the activities of the protective enzymes. The drought stress resulted in the accumulation of Pro, FAA, SS, MDA, and ABA in the pseudostems, while the IAA and GA levels decreased. Short-term drought stress led to a decrease in the SOD activity and an increase in the CAT activity, whereas the long-term drought stress led to a decrease in the POD activity. The effects of the drought stress on the SP and JA contents were negligible. The rehydration demonstrated that the short-term drought stress (7 or 14 days) did not exceed the tolerance of the Welsh onion; the substances regulating the drought tolerance were restored to normal levels after the stress conditions were relieved. In contrast, after the severe drought stress (28 days), the substances regulating the drought tolerance in the seedlings could not return to normal levels. This study provides a theoretical basis for the cultivation of the Welsh onion under early drought conditions and for breeding of drought-resistant varieties.

In Spain, the leader in pesticide sales in the European Union, a high-tech and innovative company provides services to the wine industry to optimise phytosanitary work, reduce crop losses and lower production costs. Although the nature of its business encourages the transition to a sustainable agri-food system, it also involves risks associated with uncertainty. The objective of this article was to perform the valuation of the company through the real options approach, including an expansion option, analysing whether this company will be able to increase the value of its project by expanding its activity to a larger number of vineyards. Results showed that the application of the real options approach projected a higher result than the traditional net present value method, so that if the company makes additional investments in its precision agriculture project, these will increase the value by a 15%.

Diversity in the characteristics of agricultural locations has the potential to develop. Unfortunately, the weak transfer of technology based on the characteristics of the location indicates that this potential cannot grow properly. This research aims to synthesise a technology transfer model for an agricultural technology park (ATP) by considering site-specific conditions. This cross-case study was conducted at selected ATP locations. Model synthesis uses the system development life cycle stages of initiation, analysis, and design. The resource-based view approach was broken down into several variables during the analysis stage. Three location-specific technology transfer models were successfully developed: a technology transfer model based on highland specificity, a unique technology transfer model for urban farming, and a tourism village-based model.

Seed deterioration resulting from production and storage factors is the major cause of differences in the seed vigour that results in low seedling emergence in both the laboratory and in the field. An experiment was conducted to evaluate the effect of seed invigoration by osmo-conditioning on the radicle emergence and the physiological parameters of naturally deteriorated seed lots of the true seed of shallots. The experiments were arranged in a completely randomised design with a repeated measurement for radicle emergence and a randomised complete block design for seedling vigour in the field with four replicates. The results indicated that the radicle emergence of the invigorated seed with ZnSO₄ correlated well with the seed viability and vigour parameters for seed lots stored under uncontrolled conditions. The radicle emergence of invigorated seeds stored under the uncontrolled condition were more predictive and strongly correlated with the seedling vigour parameters in the field, i.e., the field emergence, field emergence rate and mean emergence time with r = 0.968, r = 0.970 and r = –0.947, respectively. Furthermore, the coefficients of determination were significant (P < 0.05) with R² = 0.936 field emergence, R² = 0.941 field emergence rate and R² = 0.898 for the mean emergence time. Seed invigoration with 0.5% ZnSO₄ further significantly reduced the time of a single count of RE from 72 hours and 68 hours to 60 hours with field prediction rates ranging from 90–99%. It was concluded that the radicle emergence of TSS can be improved by invigorating the seeds with 0.5% ZnSO₄ thereby increasing the germination percentage, vigour index, and germination rate and reducing the mean germination time.

The study investigated whether omega-3 fatty acids could promote bacterial growth, acid and bile tolerance, and cell adhesion onto Caco-2 cells to develop a multifunctional enteric soft capsule containing probiotics and omega-3 fatty acids. The probiotic strains used were Enterococcus faecium IDCC 2102 and Bacillus coagulans IDCC 1201. Supplementation with omega-3 fatty acids did not significantly affect the growth and viability of either strain at lower concentrations (i.e. 0.5–1.0 w/w %). In comparison, the adhesion ability to Caco-2 cells of both strains was significantly increased (up to 107.5%). Furthermore, Bacillus coagulans IDCC 1201 [1 × 10¹⁰ CFU (colony forming unit) per capsule] contained together with omega-3 fatty acids (600 mg per capsule) in the enteric soft capsules showed a stability of over 95% during 12 months of storage at room temperature, which was similar to that of unencapsulated lyophilised probiotics. Thus, these results indicate that multifunctional food supplements in the form of enteric soft capsules are feasible.

The early detection of pests in plants and crops is essential for the production of good quality food. Computer vision techniques can be applied for the early detection of pests and which can minimise the pesticides used on the plants. Among many pests, white flies, mites, aphids and thrips are the most hazardous pests that affect the leaves. This paper presents an automated approach for the detection of different types of pests from leaf images of plants. The images of the plant leaves were acquired using a digital camera. Image pre-processing techniques, such as noise removal, filtering and contrast enhancement, are used for improving the quality of the images. Then, the k-means clustering method and global thresholding were used for segmenting the pests from the infected leaves. Textural features are extracted from those segmented images by statistical feature extraction methods. Artificial Neural Network (ANN) and Binary Support Vector Machine (SVM) classifiers were used to classify the unaffected leaf images from the pest affected leaf images and a multi-SVM classifier was used to identify the different types of pests.

Copper contamination stemming from copper-based pesticides poses a grave concern in vineyards and orchards, causing toxicity to soil organisms. Here, we present a comprehensive review of global data encompassing copper levels in these soils, coupled with variables such as the age of agricultural establishments, climate, soil organic matter content, soil pH, and farming practices (organic vs. conventional). The results suggest that there are three pivotal determinants driving copper content in vineyard and orchard soils: climate, the age of agricultural establishments, and soil organic matter content. It was impossible to estimate soil pH’s effect on soil copper content because of its dependence on precipitation. Copper content in vineyard and orchard soils worldwide follows a direct correlation with precipitation while inversely correlating with aridity (i.e. potential evapotranspiration divided by precipitation). Furthermore, a clear linkage emerges between farm age and increased copper content in soils globally. Intriguingly, the increased soil organic matter content has shown inverse impacts on soil copper levels. These effects of soil properties on soil copper contents were discussed in terms of copper losses from soil via surface runoff. However, no discernible disparities in soil copper content between organic and conventional farming systems were found. This worldwide survey not only underscores the established influence of climate on European vineyards but also sheds novel light on the historical legacy of copper contamination in these landscapes.

South African ‘Hass’ avocado fruit harvested early season are vulnerable to colour desynchronisation with softening during ripening, reducing their international and local market aesthetic value. So far, research has proven that ‘Hass’ avocado fruit exocarp colour desynchronization is an early season conundrum. However, there is insufficient literature on underlying factors causing exocarp colour desynchronisation. Therefore, the study aimed to investigate the ripening temperature effect on early season ‘Hass’ avocado fruit exocarp colour development and pigments during ripening. Early season ‘Hass’ avocado fruit were stored at 5.5 °C for 28 days thereafter, ripened at 16, 21 and 25 °C and evaluated for firmness, visual and objective colour (L*, C* and h°), total chlorophyll and anthocyanin content. The results showed that the visual colour of fruit ripened at 25 °C was significantly higher compared to 21 and 16 °C after 2 and 4 ripening days. This study found that ripening temperature had no significant influence on chlorophyll degradation. However, fruit ripened at 25 °C accumulated significantly higher exocarp anthocyanin concentration compared to lower ripening temperatures. In conclusion, ripening early season ‘Hass’ avocado fruit at 25 °C resulted in improved exocarp colour development and anthocyanin accumulation.

We investigated the influence of poly-glutamic acid (PGA) on ascorbic acid (AsA) and glutathione (GSH) metabolism in leaves of wheat seedlings under cadmium (Cd) stress. The results showed that Cd stress enhanced ascorbate peroxidase, dehydroascorbate reductase, monodehydroascorbate reductase, glutathione reductase, gamma-glutamylcysteine synthetase and L-galactono-1,4-lactone dehydrogenase activities, and increased AsA and GSH contents. Whereas Cd reduced AsA/dehydroascorbic acid (DHA) and GSH/oxidised glutathione (GSSG) ratios and increased malondialdehyde (MDA) content and electrolyte leakage (EL). Meanwhile, Cd stress improved Cd accumulation and nonphotochemical quenching (q_N) and decreased soil and plant analyser development (SPAD) value, net photosynthetic rate (P_n), maximum photochemical efficiency of PSII (F_v/F_m), photochemical quenching (q_P), quantum efficiency of PSII photochemistry (ΦPSII), wheat height and dry biomass. Compared to Cd alone, PGA plus Cd stress reinforced AsA and GSH metabolism via the above enzymes and increased AsA and GSH contents and their redox status. PGA plus Cd stress also decreased MDA content and EL. Besides, PGA plus Cd stress decreased Cd accumulation and increased SPAD value, P_n, q_N, F_v/F_m, q_P, ΦPSII, wheat height and dry biomass. Moreover, PGA alone showed positive effects on the indicators mentioned above. Our results clearly indicated that PGA enhanced wheat Cd tolerance by preventing Cd uptake and enhancing AsA and GSH metabolism. Therefore, PGA can be applied to enhance wheat Cd tolerance in production.

The main research objective of this study is to present the dynamics of land productivity changes in EU agriculture in the years 2012–2020 and evaluate the influence of direct subsidies received by farmers on land productivity. The source data for 2012–2020 are secondary and come from Eurostat. The research results were prepared for the entire European Union (EU-28), and divided into ‘old’ countries (EU-15) and ‘new’ countries (EU-13). The results were developed using the method of assessing convergence (in terms of differences in land productivity in individual countries) using the coefficient of variation and the relative index of changes in individual Member States in relation to the EU average over the period under study. Results showed that between 2012 and 2020, average land productivity in the EU increased by 7%. At the same time, differences in land productivity between EU countries decreased. The correlation analysis confirmed a statistically significant relationship between land productivity and the amount of direct payments per ha of agricultural land. Additionally, results of the correlation between productivity per 1 ha and the total area of agricultural land in EU countries confirmed the law of decreasing marginal productivity of land.

Seaweed has an important role in the mitigation of enteric methane (CH₄) production by ruminant animals. The utilisation and its effectiveness in enteric CH₄ reduction require a preservation process. The aim of the study was to investigate the effect of different drying processes on the effectiveness of seaweed Gracilaria spp. in reducing CH₄ production assessed through an in vitro method. Three drying techniques, sun-drying, oven-drying, and freeze-drying, were applied to produce a dried product of Glacilaria spp. Rice straw basal diets combined with concentrate at the 70 : 30% were used to test the inclusion of 4% of three differently dried products of Gracilaria spp. compared to the basal diet without seaweed (control group). Measurements were conducted on in vitro total and CH₄ gas production, nutrient degradability, ammonia (NH₃) and VFA concentration, and microbial population. Results showed that the three dried products of Gracilaria spp. significantly reduced in vitro CH₄ production compared to the control group (P ≤ 0.05). All drying techniques gave a similar effect on in vitro CH₄ reduction, but they did not affect dry matter (DM) and organic matter (OM) degradability (P ≤ 0.05) and significantly reduced neutral detergent fiber (NDF) degradability (P ≤ 0.05) compared to the control group, with oven-dried treatments having the lowest NDF degradability among the treatments. It is concluded that the three different drying techniques had similar effects on enteric CH₄ reduction.

Dietary antioxidant supplementation has become popular for managing male infertility because of its impact on the antioxidant capacity of semen. This study evaluates the effect of dietary antioxidant supplementation on the seminal characteristics of Colombian Creole horses. Semen from six Colombian Creole stallions was collected using an artificial vagina. The stallions were randomly allocated to a control group (CG) or a supplementation group (SG) that received a multi-antioxidant supplement. A repeated measures design, with a wash out period of two weeks between treatments, was used. Sperm motility and kinematics were assessed using a computer-assisted semen analysis system, membrane integrity (MI) and the acrosomal integrity (AI) of sperm were assessed using fluorescent microscopy, abnormal morphology (AM) was analysed using supravital staining, and the functionality of the sperm membrane was assessed using the hypoosmotic swelling (HOS) test. A fraction of each ejaculate sample was centrifuged to obtain seminal plasma (SP). The reactive oxygen species and the total antioxidant capacity (TAC) of the SP were measured via spectrofluorimetry. Linear models were fitted, and comparison of means was performed using Tukey’s test. Dietary antioxidant supplementation increased sperm beat cross frequency. In addition, a higher AI was found for the SG. Supplementation also increased the TAC of the SP. Individual analysis revealed that dietary antioxidant supplementation increased the total and progressive motility of the semen of two horses in the SG; however, supplementation reduced these two parameters in two other individuals compared to the CG. It can be concluded that dietary antioxidant supplementation with a nutraceutical that provides vitamins, minerals, amino acids, and omega-3 for horses can increase sperm quality as well as the TAC of SP; however, this may be subject to the individual effect of each horse.