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This article deals with the most important part of business modelling, with business process modelling. The presented process models are achieved by using common and special UML diagrams. The business process is one of the four basic business concepts creating complex business architecture and it is dynamic, structured and complicated part of the business. In business process, there are stored significant rules and knowledge of the business. Those issues could be applicable to the knowledge formalization in the agricultural business

The paper summarises main ideas concerning the structure of natural forest ecosystems at middle altitudes and documents the changes of humus forms and their chemistry in the natural forest of Žákova hora National Nature Reserve, in typical selected parts with different tree species composition: European beech - Norway spruce. The species composition, age and spatial structure are discussed and analysis of the uppermost soil layer is done in particular parts of the natural forest regeneration cycle, of different stages respectively. We compared the amount and layer composition of surface humus and basic pedochemical characteristics of holorganic and upper mineral horizons. The results document changes in the character of humus forms as a consequence of the tree species change. On the contrary, a high portion of uncertainty as for the species, age and spatial structure follows from discussion about the structure of natural forests at middle altitudes.

The paper is briefly outlining the expected changes in the economic and financial pre-conditions for Slovak farming during the period after the country's accession to the EU. The starting point of authors reasoning is the current piece of knowledge, saying that the economic performance of individual farms is widely differentiated and the ability of individual farms to meet the future challenges and to use the opportunities is very unevenly distributed among them. Also the competitiveness if a particular agricultural commodity varies. Despite of expectations concerning the price development after accession, not all commodities will be competitive on the single market. The economic situation of farms will considerably benefit from direct payments. Nevertheless, the fact that the Slovak government opted for the Single Area payment Scheme will have some adverse effects on possible gains from payments in productive regions and in farms with high output performance. In order to mitigate these redistribution effects the author is proposing to implement a different scheme of allocation of national top-up is to direct payments. In the final part of his paper the author gives a comprehensive review on payments aimed to promote rural development and payments from the Guidance section of the EAGGF targeted at enhancement of farm re-structuring.

Chrysopogon gryllus and Festuca vallesiaca are components of a number of meadow-pasture communities in Serbia. We performed the analyses of phenolics that influence quality and digestibility of grasses to a great extent. Total phenolics were measured spectrophotometrically and phenolic acids by HPLC analysis. The aboveground parts of C. gryllus contained 10.6 mg/g whereas F. vallesiaca of 21.6 mg/g total phenolics. Bound phenolics dominated over free ones in both species. The content of both free and bound p-coumaric, ferulic, p-hydroxybenzoic and syringic acid was higher in C. gryllus (6.34 mg/g) than in F. vallesiaca (3.96 mg/g). Derivatives of cinnamic acid prevailed in both species compared to the benzoic acid derivatives. Low quality of C. gryllus is connected with its high tissue phenolic acids and mediocre quality of F. vallesiaca with the high content of total phenolics that act unfavourably on digestibility of such grasses.

The condition was evaluated of tissues of declining young spruce stands in selected localities of the Orlické hory Mts. It refers to stands occurring in top parts affected by an air pollution disaster in the last century. Several types of damage of a different symptomatic character were distinguished. A high NO_x input appears to be the risk factor of spruce dieback in the Orlické hory Mts. One of the main causes in the complex of factors is the unbalanced cause of meteorological factors during the year and unbalance in mineral nutrition. The result is decrease in lignification, immaturity of tissues before the winter period and increased susceptibility of damaged tissues to parasites. Several potentially pathogenic species of micromycetes have been identified which can be considered to be significant pathogenic agents in case of declining spruce in the Orlické hory Mts., however, not a main cause.

The principal importance and global dimension of sustainable agriculture in frame of a strategy of civilization development is beyond discussion. The actual character of the topic of looking for ways to sustainable development is confirmed by the extensive worldwide program "Millennium Ecosystem Assessment" the purpose of which is to map and subsequently monitor the health of the planet Earth. The contribution is focused on various aspects of the non-substitutable role of agriculture regarding sustainable development on the level of developing and developed countries and on the regional and global level.

In the past ten years, meat and meat products consumption has changed dramatically. It has been caused by price and non-price factors. This article gives a brief recapitulation of the development of the consumption of meat and meat products and identifies the key factors which, in the past ten years, have had a major impact on this development. This article presents results of the secondary data analysis and the results of an inquiry into meat and meat products consumer behaviour carried out in the late 2004.

Hair roots are a very attractive source of DNA for microsatellite-based parentage control of breeding animals. However, unlike blood samples, irregular DNA typing results have been observed in assays utilizing hair follicles. The amount of starting material and DNA preparation method are the crucial factors. In order to improve DNA typing results for horse hair roots, two quick preparation methods and additional purification steps were evaluated. PCR efficiency for each approach was expressed as percentage of samples with complete DNA profiles for 12 horse microsatellites. The lowest percentage (22%) of complete DNA profiles was obtained for samples prepared by the proteinase K digestion method. The best genotyping results (94%) were achieved after phenol-chloroform extraction of DNA from samples prepared by the proteinase K digestion method. Direct cleanup of DNA samples with an ethanol-sodium acetate mixture gave comparably good results of microsatellite genotyping (91%). DNA preparation from hair roots with proteinase K digestion followed by DNA purification with ethanol was chosen as the most efficient approach for horse DNA typing under parentage testing.

The basic principle from which the Czech National Forestry Programme proceeds is the management of forests in a permanently sustainable manner whilst limiting the administrative interference of the state to the unavoidable minimum under the circumstances of the motivating operation of state forestry policy for the support of public interests and whilst increasing the responsibility of forest owners for their property. The National Forestry Programme is supposed to be an interdepartmental and intersectoral programme respecting not only the needs for the branch development of forest management, but also emphasising the place of forests in the environment and landscape creation, non-production functions of forests, the importance of the forest as a renewable source of ecologically advantageous raw material and the significance of the use and processing of wood for the economy of the country and thus for the society as such. The first idea of National Forestry Programme has been presented in 1993. The current version of the Programme has been conceived for the period 2003-2006. In addition to the basic information about the current state of forests and forestry management in the Czech Republic, it contains chapters with themes focussing on forestry problem issues.

Contamination of cell cultures and sera used for animal virus propagation with mycoplasmas represents a serious problem, especially in virology. Therefore specific control measures must be used. To achieve this we introduced PCR for the detection of mycoplasma species in cell cultures and compared its results with ELISA and microbiological culture. Seven mycoplasma species which are the most common contaminants of cell lines (Mycoplasma arginini, M. fermentans, M. hyorhinis, M. bovis, M. orale, M. hominis, and Acholeplasma laidlawii) were used to verify the method. Then we assessed five selected cell lines and three bovine sera by the PCR, ELISA and culture methods and compared the results. PCR was positive for all of the mycoplasma species tested. ELISA kit used (Mycoplasma detection kit, Roche, Germany) allowed detection of only four species of contaminating mycoplasmas (Acholeplasma laidlawii, Mycoplasma arginini, M. hyorhinis, and M. orale). All the methods detected contamination of the VERO and RK13 cell lines. The agents of contamination were determined by the species-specific ELISA kit as Mycoplasma arginini and M. orale, respectively. Other cell lines and sera tested were not contaminated with mycoplasma. The results confirmed that the PCR method used in the present study is a sensitive, fast and specific detection method of mycoplasma contaminations and is suitable for routine mycoplasma detection in cell cultures and bovine sera.

Retailers are the strong group both in South Bohemia and in Upper Austria. The development of this sphere is one of the development programmes priorities of both regions. South Bohemia and Upper Austria, especially Muhlviertel region, are areas with a similar economic structure, which is an excellent presupposition for mutual cooperation. An advantage will be the improvement of marketing activity and information transfer. A better technical equipment and its full exploitation will be possible. The problems, however, which could arise from cooperation with foreign partners, should not be underestimated. Not only the language barrier is concerned but also different techniques, methods and expectations that might be caused by different customs and interests of the partners. An important aid to the entrepreneurs, either concerning consultant service, establishing contacts or further education, are economic chambers and other institutions concerned with retail business development.

The aim of this study was to investigate the contamination of beer of Slovak origin with fumonisins. A suitable analytical procedure was suggested - the limit of detection at the level close to 1 µg/l was achieved for both fumonisins B₁ and B₂. The recovery was determined at 93% for fumonisin B₁ and at 78% for fumonisin B₂. Fluorescence detection was used after derivatisation with a mixture of o-phthaldialdehyde and 2-mercaptoethanol. Phosphate buffer usually applied resulted in a poor separation of derivatised fumonisins. Peak splitting was observed depending on the pH of the eluent. The pH value of 2.6 was found suitable for the peak splitting elimination. A convenient gradient elution metod was suggested avoiding the possible interference in fumonisin contents determination. For the preparation of samples, immunoaffinity cleaning procedure was applied. Beer samples from all domestic producers were analysed. The content of fumonisins determined was under the limit of detection in all cases. All the beers tested were produced from the barley grown in 2003.

The covariance structure was estimated by REML for a joint genetic evaluation of production and reproduction traits for Czech Landrace (58 258 records) and Czech Large White (167 161 records) pigs using four-trait animal models. The following traits were analysed: lean mean percentage at the end of the performance test in the field, estimated from ultrasonic measurements unadjusted for live weight (LM), average daily gain in field test (in g/day) calculated as weight at the end of the test divided by age at the end of the test (ADG), number of piglets born alive in parity 1 (NBA1) and number of piglets born alive in parity 2 and subsequent parities (NBA2+). The heritabilities were in the range from 0.30 to 0.37 for LM, from 0.13 to 0.18 for ADG, from 0.09 to 0.13 for NBA1 and from 0.10 to 0.14 for NBA2+, depending on the breed and on the model (herd-year-season random or fixed). Genetic correlations between production and reproduction traits were estimated to be non-zero. Correlations between traits caused by the herd-year-season effect were mostly positive. As a general conclusion, the joint genetic evaluation of production and reproduction traits is recommended. The herd-year-season effect should be preferably considered as random.

Alder decline along watercourses is one of the marked manifestations of tree decline in the Czech Republic. Local decline of alder trees is documented in the Czech Republic for about 15 years. The aim of this paper is to evaluate causes of alder decline and assess health conditions of other species of riparian stands at 2 localities on the medium reach of the Lužnice River. Effects were studied of abiotic and biotic stressors on the health condition of tree species. No fungal pathogen was found in tissues of declining alders which would be present in all trees and which could be considered to be the main causal agent of the decline. Even an occurrence of the causal agent of alder decline named alder-Phytophthora has not been proved. Tree-ring analyses demonstrated decreasing trends of an increment in alders; however, an abiotic or biotic factor showing direct effects on the fluctuation of tree ring dimensions has not been positively determined. A marked role in the alder decline is demonstrated particularly by abiotic factors accompanied by the secondary activation of some pathogens. Generally, the phenomenon can be named as polyetiologic decline.

The incorporation of Czech agrarian sector in the Common Agricultural Policy of the EU means also the application of so called "European Model of Agriculture" which reacts to a basic requirement of socially balanced and sustainable agriculture which contributes to maintenance and an improvement of the European agricultural cultural landscape. In connection with it, a new strategy of agrarian policy was set according to which the orientation of Czech agrarian sector changes in a principal way, which was focused up to now only on the production function and also its other functions become more important, above all in the environmental and social, so non-production area. One of the aspects of multifunctional agriculture is an organic agriculture, a system which fulfills visions of sustainable agriculture.

The objective of the paper was to verify the convenience of the application of three standardized detection systems: disk diffusion method, Delvotest SP and Penzym S 100 to control the antibiotic residues in goat's milk (β-lactam antibiotics and cephalosporins, aminoglycosides, tetracyclines, macrolides and others). It has been found that despite of certain specificity of goat's milk versus cow's milk the values of the majority of detection limits mutually correspond approximately to 90 %. The sensitivity of tests manifested itself in the following order: Penzym S 100 > Delvotest SP > disk diffusion method (the sensitivity was even several times lower). Inasmuch as the treatment of mastitis is carried out by using β-lactam antibiotics and cephalosporins, the above-indicated rapid methods (especially Penzym S 100 and Delvotest SP) can be recommended for the routine purposes of accomplishing a rapid hygienic control of goat's milk.

The paper evaluates the growth, structure, production, quality, leaf area index and dendrochronology of 100 years old Austrian pine (Pinus nigra ARNOLD) monoculture situated in the forest type group Fageto-Quercetum in the locality Horné Lefantovce (Nitrianska Streda Forest District, Topoľčianky Forest Enterprise). Codominant trees, trees with stem of high quality, with medium-sized crown, medium dense and straight crown are the most abundant in the stand. The tree number in the stand is 1,024 trees/ha, basal area 51.75 m2/ha, growing stock 571.56 m3/ha, aboveground biomass stock 348.76 t/ha and leaf area index 21.85 ha/ha. Dendrochronological analyses examined the response in individual sample trees and minimum annual ring width was found in 1920, 1922, 1925, 1929, 1933, 1938, 1944, 1946, 1947, 1949, 1952, 1954, 1963, 1968, 1976, 1982, 1990, 1993, 1998, 2001. Marked maximum values of annual ring width in the years 1919, 1923, 1926, 1930, 1936, 1937, 1941, 1948, 1951, 1955, 1959, 1966, 1972, 1975, 1980, 1985, 1997 were found as a positive productive feature. Beginning in the year 1993, dry Austrian pine trees occurred in the stand as a result of the fungal infection by Sphaeropsis sapinea (Fr.) Dyko et Sutton.

The important part of the increase of regional potential is the farming of the land fund. The economic conditions for its utilisation have been already prepared. They are based especially on the drawing of funds of the European Union, namely for the period after the accession of the Czech Republic to the European Union and for the years 2007-2013. The measures concerning the land are included in the prepared programme documentation, especially in the Horizontal Rural Development Plan (HRDP) and in the Operational Programme "Agriculture". The farmers will obtain the direct payment per area (SAPS) in addition to supports on foregoing measures. The requirements for environment-friendly farming of land will be increased in the future. The economic conditions for next period will concentrate on these priorities. It will concern the securing of public benefit contributing to the sustainable development of rural areas. The project assurance of these trends and their regional monitoring and evaluation will be important.

The following characteristics were determined in raw and popped amaranth grain: crude protein (158.1 and 168.5 g/kg), ether extract (71.5 and 69.4 g/kg), neutral detergent fibre (NDF) (99.2 and 111.8 g/kg), cellulose (86.6 and 60.0 g/kg) and essential amino acids (Cys 4.2 and 4.1, Thr 6.0 and 6.5, Ala 8.8 and 9.2, Val 6.8 and 7.4, Ile 5.2 and 5.6, Lys 9.2 and 8.8, Arg 12.8 and 14.2 g/kg). In vitro protein digestibility was 68.1 and 50.6% in raw and popped amaranth grain, respectively. In balance experiments with broiler chickens ROSS 308 the following coefficients of apparent digestibility (%) were determined for control and experimental diets containing 0, 10% raw and 10% popped amaranth: crude protein 85.4, 86.5 and 83.0, ether extract 88.3, 88.2 and 86.1, NDF 21.2, 27.6 and 15.9, cellulose 25.0, 38.4 and 36.3, nitrogen free extractives 76.1, 82.6 and 81.1, organic matter 77.3, 81.8 and 80.6, gross energy 77.5, 80.6 and 78.2.

Measurement of the ammonia emissions by two different types of sensors (PHOTO ACOUSTICS analyzer INNOVA and SOLID STATE sensor SP-53) was carried out in laboratory with one material of litter, wood-shavings (with dry or wet material). The initial ammonia concentration and humidity of material were changed. The output data of these sensors were continually saved a processed. A small difference was found out between the output data of these sensors. The difference depends not only on ammonia concentration and humidity of material but also on the duration time from the beginning of measurement. All experiments of ammonia concentration were carried out with three initial values of humidity and ammonia concentrations within used material. The decrease of ammonia concentration (its velocity) in investigated material has been measured with laboratory device and registered for twelve or more hours during each experiment.

3-amino-2-oxazolidinone (AOZ) is a tissue bound toxic metabolite derived from the nitrofuran antibiotic, furazolidone. AOZ is detected in the derivatised form of 3-{[(2-nitrophenyl) methylene] amino}-2-oxa-zolidinone (NP AOZ). 3-{[(3-carboxyphenyl)-methylene] amino-2-oxazolidinone (CP AOZ) was used as the immunising hapten for the production of monoclonal antibodies against NP AOZ. Monoclonal antibodies were produced using hybridomas from the fusion of murine myeloma cells and spleen cells isolated from BALB/c mice immunised with CP AOZ-ethylenediamine-human serum albumin (CP AOZ-ed-HSA). The antibody production in ascitic fluids from clones 3B8/2B9 and 2D11/A4 was monitored during a 16 month period. Repeated cultures of these hybridomas, followed by injection into mice and cloning did not change the assay parameters. Clone 2D11/A4 exhibited long term stability in antibody production throughout the experiment whereas clone 3B8/2B9 demonstrated variability in particular antibody yields whilst retaining assay sensitivity. Reasons for this production variability in clones are discussed. In an optimised direct ELISA format, the antibodies exhibited a 50% binding inhibition in the range of 0.52-1.15 ng/ml with NP AOZ (0.22 -0.50 ng/ml, respective AOZ equivalents) and showed high specificity towards this analyte. The sensitivity of monoclonal antibodies incorporated into the ELISA is compatible with the European Union MRLP and is currently in use for routine analysis.

Ochratoxin A is a very common mycotoxin which can be found rather often, predominantly in various cereal materials and in products from this type of plants. Our aim was to apply an analytical procedure with a suitable detection level of ochratoxin A for its estimation in beer. The detection level of the method suggested was close to 0.001 µg/kg. The analytical procedure is based on HPLC separation with fluorescence detection. The application of this method is demonstrated and analytical results obtained with beer of domestic provenience are reported.

The effects of three doses of vitamin C (100, 200 and 300 mg/kg) added to the feed of reproductive pheasants on egg production, egg fertility and parameters of hatchability were investigated. In experiments I (1999) and II (2000) birds were kept in outdoor aviaries. In 2000, air temperatures recorded on the farm during the experiment were high (25-30^oC), differing considerably from those recorded in the preceding years. In experiment I no statistically significant differences were shown between the control pheasants and those supplemented with 100 and 200 mg/kg vitamin C in terms of egg production, egg fertility and parameters of hatchability. The poorest results were found in the group of birds receiving a feed supplemented with 300 mg/kg vitamin C. In experiment II, statistically higher egg production and egg fertility were noted, compared to the control group, in the groups supplemented with 100 and 200 mg/kg vitamin C. Compared to the control group, pheasants from these groups were also characterized by higher hatchability from set eggs (by approx. 15 percentage units) and fertilized eggs (by approx. 9 percentage units) and smaller number of unhatched chicks and dead embryos after day 10 of incubation. In experiment II, the beneficial effect of 100 and 200 mg supplements of vitamin C on the studied parameters could result from the soothing action of the vitamin on the effects of heat stress. The present results justify the prophylactic use of vitamin C supplement at 100 mg/kg feed in the nutrition of reproductive pheasants reared in aviaries. Under heat stress (high air temperatures) increasing the dose of this vitamin to 200 mg/kg feed seems beneficial.

The effects of soil conditioners TerraCottem, Frisol, Bio-algeen and fertilisers Silvamix Forte and Cererit on the survival rate and growth of European ash seedlings until the stage called established plantation were observed on two experimental plots with different anthropogenic substrates. Eight-year experiments showed that under the given conditions the tested agents fulfilled the declared effects only partially. The application of TerraCottem was statistically insignificant both in the survival rate and in the growth. In the applied dose, Frisol manifested itself as an agent that may cause problems with the survival of plants, but at the same time also as a preparation improving the overall growth of young plants statistically significantly. Its effective dosage largely depends on the soil character. Bio-algeen had a very good effect on the survival rate of plants, but it did not surpass the control plants in the initial growth of plants. Silvamix Forte did not cause the mortality of seedlings and influenced the growth of plants statistically significantly for 1-3 years. In the applied dose, Cererit caused a high mortality of seedlings. The application of soil conditioners and Silvamix Forte did not markedly accelerate the growth and development until the stage of the established plantation. Until that time, the costs of the application of conditioners were higher by 4-13% as compared with the costs in the control plants.

An explanation of the 'boom-bust' cycle of resistance breeding was provided by the gene-for-gene relationship between a pathogen and its host. Despite this understanding, most R genes continued to be deployed singly and resistance has been ephemeral. The reasons for breeding 'single R gene' varieties are discussed. Alternative strategies for the deployment of R genes and the use of quantitative race non-specific resistance have been advocated in order to obtain durable resistance. The feasibility of both of these approaches is discussed taking into account the impact of technologies such as plant transformation and marker-assisted selection. A change in focus from durability of the plant phenotype to that of the crop phenotype is advocated.

In recent years, molecular markers have been developed based on the more detailed knowledge of genome structure. Considerable emphasis has been laid on the use of molecular markers in practical breeding and genotype identification. This review attempts to give an account of different molecular markers currently available for genome mapping and for tagging different traits - restriction fragment length polymorphisms (RFLPs), random amplified polymorphic DNAs (RAPDs), amplified fragment length polymorphisms (AFLPs) and microsatellites. Other markers, expressed sequence tags (ESTs) and single nucleotide polymorphisms (SNPs) are also mentioned. The importance of structural, functional genomic and comparative mapping is also discussed.

Haemorrhagic septicaemia (HS) is an endemic disease in most countries of Asia and sub-Saharan Africa. Within the Asian Region, countries can be classified into three categories, on the basis of incidence and distribution of the disease; these are respectively countries where the disease is endemic or sporadic, clinically suspected but not confirmed, or free. Economic losses due to HS are not only confined to losses to the animal industry, but also rice production on account of its high prevalence among draught animals used in rice fields. Only a few attempts have been made to estimate economic losses, the methodologies used in different countries have varied, and many are not based on active surveillance, and a consideration of all components of direct and indirect losses. Most Asian countries rank HS as the most important contagious disease or the most important bacterial disease in cattle and buffaloes. Resource allocation for prevention and control of HS nationally or internationally will evidently depend on a correct estimate of its economic impact. The key factors in prevention and control would be timely and correct reporting, accurate and rapid diagnosis, strategic use of vaccines with the attainment of a high coverage where necessary with a high quality vaccine. National level activities geared towards attainment of these objectives may be with advantage supported and strengthened by international organisations involved in animal health. The present paper attempts to review aspects related to the epidemiology, control and containment of HS in Asia and, proposes some key issues on which a regional programme for HS control in this continent should be centred.

The present study was an in vitro analysis of the dynamics of bovine mammary gland neutrophil apop­tosis based on the detection of morphological changes. The neutrophils were isolated from mammary glands of five virgin heifers. The mammary glands were lavaged, the suspensions were then bacteriologically examined, and total and differential cell counts were made. The cells were cultivated in vitro for 4 hours. After 2, 3 and 4 hours of cultivation, they were panoptically stained, and the proportions of apoptotic neutrophils and trypan blue positive neutrophils were determined. Neutrophil apoptosis and impaired cytoplasmic membrane integrity of neutrophils were already observed in the mammary gland lavages (11.9% and 0.8%, respectively). During the cultivation, a progressive increase in the number of apoptotic neutrophils in various stages of apoptosis - karyopyknosis, zeiosis and apoptotic bodies - was observed. Karyopyknotic neutrophils represented a dominant part of the apoptotic neutrophil population in the course of the whole cultivation. The most intensive increase was observed in zeiosis, whereas the levels of apoptotic bodies remained the same. After 4 hours of cultivation, 31.7% apoptotic neutrophils and 9.8% trypan blue positive neutrophils (i.e. Secondary necrotic cells) were found. The results of this work show that spontaneous apoptosis and secondary neutrophil necrosis must be taken into account during in vitro cultivations of bovine mammary gland neutrophils.

Resistance or susceptibility in barley to powdery mildew is determined at the single cell level. The fungus attempt to penetrate the plant cell wall and the attacked barley epidermal cell tries to prevent this by reinforcing its cell wall. However, this defence is only partially efficient and a number of fungal penetration attempts will succeed, leading to a mixture of infected and uninfected epidermal cells on the leaf. This makes it impossible to relate powdery mildew induced gene expression to resistance or susceptibility. Using glass micro-capillaries and micromanipulation we extracted contents from single barley epidermal cells. Three situations were considered: A) the barley cell resisted fungal penetration, B) the barley cell was infected and C) the barley cell was uninfected. The content of mRNA in the micro-extracts was purified and cDNA libraries were produced. Verification of the integrity of these libraries was done by gene specific PCR of selected genes and dot-blot hybridisation. Powdery mildew attack induces expression of a series of pathogenesis related (PR) genes in barley. Using dot-blot technique the expression of these genes was examined in the sampled cells. Notably, the PR genes were only induced in the cells that resisted powdery mildew penetration, whereas this activation was not observed in cells that were successfully invaded by the fungus. This indicates that the PR genes are involved in the processes leading to prevention of fungal penetration and that the fungus is able to prevent induction or suppress expression of the PR genes when barley cells are successfully infected.