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Results 31 to 60 of 1498:

Effect of logging residues management on the distribution of potentially toxic elements in soils of large-scale clearcuts resulting from bark beetle forest damageOriginal Paper

Lenka Pavlù, Marek Kuèírek, Václav Tejnecký, Ondøej Drábek, Petra Vokurková, Lubo¹ Borùvka, Martin Valtera, Vìra Fadrhonsová, Radek Novotný

Soil & Water Res., 2026, 21(1):43-51 | DOI: 10.17221/119/2025-SWR

This research focuses on the effects of large-scale clearcuts resulting from salvage logging after spruce (Picea abies) forest dieback caused by an extreme bark beetle infestation, and on the effect of logging residues management (chopping vs. clearing) on the distribution of potentially toxic elements (PTEs) in soil. Pseudo-total contents of Cd, Cr, Cu, Ni, Pb and Zn were determined in soil samples collected separately from the organic (F+H) and mineral (0–10, 10–20, and 20–30 cm depths) soil layers. The distribution of elements was influenced mainly by sampling locality and position in the soil profile. In general, the contents of Cd, Ni and Cr were higher in the mineral layers, whereas Pb was more concentrated in the FH layer. A significant effect of logging residues management on the distribution of PTEs was observed only for Pb and Zn. We expect that the relative decrease of Pb and increase of Zn contents in the “chopped” treatment was mostly due to the higher input of mineral soil and wood residues to the FH layer. Since the stand was harvested relatively recently, the effects of soil preparation have probably outweighed those of spreading or removing logging residues.

An investigation of the presence of Xylella fastidiosa in Cicadomorpha specimens collected in different habitats in PolandOriginal Paper

Monika Ka³u¿na, Gra¿yna Soika, Wojciech Warabieda

[Ahead of Print]CAAS Agricultural Journals, X:X | DOI: 10.17221/207/2024-PPS

A quarantine organism, the bacterium Xylella fastidiosa (Xf), is a xylem-inhabiting, vector-transmitted, Gram-negative, and very slow-growing bacterium in the Lysobacteraceae (earlier Xanthomonadaceae) family. The spreading of X. fastidiosa over long distances occurs mainly via import/export human-mediated transportation of mainly latently or symptomatically infected plant material. Short-distance distribution is usually by xylem sap-feeding insects. Until now, the presence of X. fastidiosa has not been reported or studied in Poland. During our study, over 500 individuals from the four families: Cicadellidae, Aphrophoridae, Delphacidae and Membracidae were collected in different geographical regions of Poland. Real-time PCR with primers for rimM gene and nested PCR to detect X. fastidiosa, using DNA extracted directly from selected insects known as potential vectors of X. fastidiosa, did not confirm the bacterium's presence in these insects.

Forest certification management experience in Asia Pacific and Europe: A case study of Malaysia and SwedenOriginal Paper

Shah Badri Mohd Nor, Ahmad Fariz Mohamed, Shamsul Khamis

J. For. Sci., 2025, 71(6):269-285 | DOI: 10.17221/3/2025-JFS

The forest certification is a voluntary programme to ensure that the timber harvested from forests is processed in a sustainable manner by following specific standards. The Programme for the Endorsement of Forest Certification (PEFC) was introduced in 1999 using the bottom-up approach, and it gives flexibility for a country to set up their own PEFC standard based on current practices in sustainable forest management (SFM), regulation, and governance. This practice has led to the development of various PEFC standards for each country; thus, the impact of PEFC certification differs from country to country. In accordance with that, this study aimed to evaluate the PEFC standard application in developed and developing countries, namely in Sweden and Malaysia. The study also analysed the impact of forest certification on governance and economic aspects within Asia Pacific and Europe. The outcome of this study indicates that the PEFC standards developed by these countries showed similarities; for example, both countries are focusing on social and environmental requirements. Meanwhile, the differences were shown in the criteria, terms used, and the focus area. The impact analysis exhibited that countries are facing similar issues, especially market access and premium prices. This study revealed that each country develops different standards based on current practices, forest diversification, and regulations.

Bridging credit gaps for sustainable agriculture: The role of rural savings and credit cooperatives among smallholder farmersOriginal Paper

Gershom Endelani Mwalupaso, Shangao Wang, Xianhui Geng, Shadrack Kipkogei

Agric. Econ. - Czech, 2025, 71(5):254-272 | DOI: 10.17221/436/2024-AGRICECON

Despite the recognised benefits of climate smart agriculture (CSA) in enhancing farmers' adaptive capacity to climate risks, adoption rates remain low in Sub-Saharan Africa. This disparity can be attributed, in part, to the significant challenges smallholder farmers face in accessing credit from the formal financial sector. In response, Rural Saving and Credit Cooperatives (RUSACCOs) have emerged as crucial sources of funding for both household expenses and agricultural activities. However, despite their increasing importance in improving financial inclusion, little is known about whether participation in RUSACCOs can help alleviate existing credit constraints and promote the adoption of CSA among smallholder farmers. To address this knowledge gap, we employ a recursive bivariate probit (RBP) and propensity score matching (PSM) analysis using data from 400 randomly selected smallholder farmers in Zambia. The analysis controls for three main sources of endogeneity: program placement, endogenous covariates, and self-selection. Our findings indicate that participation in RUSACCOs has the potential to mitigate farmers' credit constraints by 42% and facilitate CSA adoption by 25%. Notably, the alleviation of existing credit constraints is associated with a 14% increase in CSA adoption. These results underscore the previously overlooked role of RUSACCOs in promoting agricultural sustainability. By effectively addressing financial inclusion barriers and providing access to practical agricultural knowledge, RUSACCOs can contribute to reducing the vulnerability of agriculture while fostering sustainable production. Our study suggests that repurposing RUSACCOs to emphasise financial inclusion and promote access to agricultural learning platforms can yield triple benefits: agricultural, environmental, and livelihood sustainability.

Multi-trait evaluation of oilseed rape varietiesOriginal Paper

Katarzyna Waszak, Konrad Bana¶, Jacek Broniarz, Tomasz Lenartowicz, Henryk Bujak, Agnieszka £acka, Marcin Przystalski

Plant Soil Environ., 2025, 71(12):860-872 | DOI: 10.17221/337/2025-PSE

The multifaceted nature of agricultural management and environmental factors complicates the production of winter oilseed rape (Brassica napus L.). This study evaluated 25 varieties (21 hybrids and four populations) in three growing seasons (2020/21, 2021/22 and 2022/23) in Poland. The focus was on yield, fat content, and resistance to Sclerotinia sclerotiorum. The analyses revealed significant variability among the varieties, with the hybrids performing better consistently in terms of yield and fat content. The level of resistance to Sclerotinia was similar in hybrid and population varieties. Furthermore, DK Excited was found to be the highest-yielding variety, while Duke had the highest fat content. Derrick was the most resistant to S. sclerotiorum. Advocat and Dynamic were identified as the best varieties. In the analysed series of field trials, yield was found to be affected by high temperatures and a lack of rainfall in March, June, and July. For fat content, a lack of rainfall in July was the main limiting factor.

The new EU Timber Regulation 2023/1115 and its legal implications for the Czech forestry and timber sectorReview

Petra Hlaváèková, Martin Cempírek, David Bøezina

J. For. Sci., 2025, 71(12):575-588 | DOI: 10.17221/82/2025-JFS

Regulation (EU) 2023/1115 on deforestation-free products (EUDR) establishes a substantially revised legal framework that extends beyond the previous EU Timber Regulation (EUTR 995/2010) by integrating legality, sustainability, and traceability obligations. This article provides a legal and comparative assessment of the implications of the EUDR for the Czech forestry and timber sector. The methodological approach combines doctrinal interpretation of EU legislation, comparative analysis of national implementation models, and an examination of relevant case law. Experiences from selected EU Member States are used to illustrate institutional, technological, and administrative challenges linked to EUDR implementation. The results indicate that, although the Czech Republic has an established regulatory framework under Act No. 251/2025 Coll., effective compliance with the EUDR will require improved coordination among supervisory authorities and the development of a unified digital platform for submitting and verifying due diligence declarations. Evidence from other European countries shows that integrated monitoring systems and the use of third-party certification can enhance both efficiency and transparency. The study concludes that the successful implementation of the EUDR in the Czech context will depend on institutional capacity, digital readiness, and the ability of forest managers and traders to adapt to more stringent environmental and traceability requirements.

Antimicrobial properties of secondary metabolites of Cannabis sativa: A promising natural alternative for livestock healthReview

Tereza Paulová, Karel Novák, Eva Pìchouèková

Czech J. Anim. Sci., 2025, 70(9):357-382 | DOI: 10.17221/85/2025-CJAS


In addition to their practical importance as a medicinal plant, animal feed and a source of materials for the textile and construction industry, industrial varieties of Cannabis sativa L. (hemp in a wider sense) provide an alternative for controlling infectious diseases in livestock. Despite the genetic divergence between two primary groups of cannabis, i.e. medicinal cannabis and technical hemp, hemp plants also produce a wide spectrum of secondary metabolites. These include the main classes of cannabinoids and terpenoids, as well as representatives of flavonoids, stilbenoids, steroids, alkaloids, spiroindans, dihydrophenanthrenes, and lignanamides. Many of them exhibit antibiotic activity which can substitute or complement the use of traditional antibiotics in animal husbandry. For example, the cannabinoid fraction exhibits activity against the Gram-positive bacteria and some fungi. While the activity against Gram-negative bacteria is not characteristic of cannabinoids, these pathogens can still be affected by hemp terpenoids and flavonoids. The synergy among the secondary metabolite fractions or between the hemp metabolites and traditional antibiotics is also a favourable factor. The search for alternatives to traditional antibiotics is further driven by the increasing prevalence of genetically determined antibiotic resistance among veterinary pathogens, which poses the additional risk of transferring resistance traits to the human pathogens. The content of antibiotically active compounds in hemp can be enhanced through selection among existing genotypes, targeted breeding, cultivation conditions, and even by specific elicitation of secondary metabolites with the natural antibiotic function in the disease resistance of the plant. The switch to hemp metabolites is also supported by their compatibility as natural components of plant-based animal feed, and by favourable economic considerations.

Multi-objective optimisation and synergistic mechanisms of expansive soil improvement using organic fertiliser, slow-release fertiliser, and rice strawOriginal Paper

Yonggang Huang, Hongri Zhang, Xinzhong Wang, Yuexing Wu, Xianliang Tan, Kang Xiong

Soil & Water Res., 2026, 21(1):20-33 | DOI: 10.17221/47/2025-SWR

This study systematically investigated the synergistic improvement of expansive soil using organic fertiliser (OF), slow-release fertiliser (SRF), and rice straw (RS) through Box-Behnken design (BBD) and response surface methodology (RSM). Key findings include: the quadratic models demonstrated high statistical significance (root density: R² = 0.765, F = 25.84; shear strength: R² = 0.885, F = 18.65; swelling rate: R² = 0.20, F = 15.23; all P < 0.001) with low prediction errors (root content: ± 0.08 mg/cm3; shear strength: ± 0.58 kPa; swelling rate: ± 0.38%); The combination of 12.30% OF + 0.7 kg/m3 SRF + 0.4% RS achieved 58% improvement in shear strength, 32% improvement in root content, 42.7% reduction in swelling rate; OF exhibited negative linear effects on root density (β = – 0.18, P = 0.002) with >10% dosage reducing root growth by 9.0%; SRF showed positive linear impacts on shear strength (β = +0.25, P = 0.001) and root density (β = + 0.12, P = 0.023); RS enhanced shear strength below 0.5% (β = + 0.08, P = 0.042) but impaired root density due to pore clogging (β = – 0.15, P = 0.008). The optimised formulation, validated by triplicate centre-point tests (coefficient of variation ≤ 2.1%), is recommended for slope stabilisation while limiting OF to ≤ 10% to prevent performance degradation. This data-driven approach provides actionable insights for balancing agricultural waste utilisation and geotechnical performance in expansive soil improvement.

Palliative care for cancer patients in veterinary medicineReview

CSF Repetti, JR Rueda, CD Porto, R Prevedello Franco, RJS Girio, FFR Manhoso, IB Costa

Vet Med - Czech, 2023, 68(1):2-10 | DOI: 10.17221/76/2022-VETMED


Neoplasms account for approximately half of the deaths of dogs over ten years of age. This finding, associated with the fact that canine cancer patients will often suffer from the consequences of the systemic spread of the tumour and paraneoplastic syndromes, shows the importance of understanding palliative care by veterinarians and owners. In view of this, this review aims to address palliative care that can be used in veterinary medicine to promote the patient’s well-being and quality of life.

Forestry violations as a global issue of legal regulation in the field of forest control and supervision: the Krasnodar territory and the Republic of Bashkortostan case studyOriginal Paper

Radmir Iksanov, Olga Khalikova, Igor Vladimirov, Ravil Gizzatullin, Regina Baiturina, Vitaliy Kovshov, Aigul Selezneva, Sofya Khasanova

J. For. Sci., 2021, 67(6):272-284 | DOI: 10.17221/99/2020-JFS

Control and supervision activities in the field of forest management are a type of activity of state authorities to detect, prevent forest violations. The purpose of this study is to analyse the dynamics and nature of violations in the forest sector on the example and in comparison of such regions of Russia as the Krasnodar territory and the Republic of Bashkortostan. The research methodology is selected taking into account the characteristics of the object under study. With the assistance of employees of the environmental inspectorate, search operations were organized in the areas where the greatest amount of forest damage occurs in the studied regions. The authors concluded that one of the reasons for the existing problems is the lack of interest of the state in the development of this sector of economy. The paper focuses on specific types of forest violations and the problems of their prevention. The authors believe that illegal logging belongs to the most common and socially dangerous forest violations. The article concludes that when adopting new regulations for control and supervisory activities in the field of forest management, the legislator must take into account requirements of administrative reform, regulatory guillotine, and risk-based approach.

Pedo-climatic predictions and reality of sunflower (Helianthus annuus L.) growing in SlovakiaOriginal Paper

Jozef Vilèek, Matú¹ Maxin, Monika Lörincová, Miroslav Kudla

Plant Soil Environ., 2023, 69(11):545-553 | DOI: 10.17221/323/2023-PSE

This work aims to differentiate the rural land of Slovakia in view of the possibility of effective sunflower growing. The differentiation is based on pedo-climatic and production-economic parameters. Soil categorisation took into account the correlation between the site properties (soil and climatic conditions) and the biological and agrotechnical requirements of the crops. Sunflower requirements were included in yield databases using software filters such that a given site property excluded or limited sunflower growing, which was reflected in predicted production. The prediction was subsequently interpolated into four suitability categories: soils unsuitable for sunflower growing, less suitable soils, suitable soils and very suitable soils. A map of categories of soil suitability for sunflower growing was created using a Geographic Information System on the distribution of soil parameters in Slovakia. According to our calculation in Slovakia, 18.8% of farmland is very suitable for sunflower growing, 24.9% is suitable, 16.6% is less suitable, and 39.7% is unsuitable for sunflower growing. These categories are characterised and specified in detail in the paper in terms of geographical, soil, climatic, production and economic parameters. The analysis of the actual sowing of sunflowers between 2018 and 2021 showed that 51% of the areas were located in very suitable soils, 32% in suitable, 10% in less suitable soils and 7% in unsuitable soils for cultivation.

Pyrethroid susceptibility and oxidative detoxification mechanism in Colorado potato beetle and western corn rootwormOriginal Paper

Daria Dworzañska, Joanna Zamojska, Pawe³ Wêgorek, Pawe³ K. Bere¶, S³awomir Drzewiecki

Plant Protect. Sci., 2023, 59(2):174-184 | DOI: 10.17221/53/2022-PPS

The Colorado potato beetle (CPB) and the western corn rootworm (WCR) are among the world’s most expansive insect pests. The aim of the study was to assess, for the first time in Poland, the levels of susceptibility to deltamethrin and tau-fluvalinate in the WCR and to determine the current status of susceptibility to those active substances in the CPB. In addition, the role of oxidative metabolism in the detoxification of pyrethroids was determined using piperonyl butoxide (PBO). The study found pyrethroid resistance in the CPB populations. The WCR populations exhibited “high susceptibility”, “susceptibility”, “low resistance” and “medium resistance” to the pyrethroids tested. Experiments using PBO showed that oxidative enzymes are involved in the deltamethrin detoxification mechanism in the CPB, although the effectiveness of this mechanism in Poland is decreasing. PBO was found to be highly toxic to the WCR populations. The findings from this study should be taken into account when developing a strategy to prevent the development of resistance in the pest species. The results for the toxicity of PBO to Polish WCR populations demonstrated that the enzymes inhibited by PBO are involved in important metabolic processes, which should be considered when developing innovative control methods for this pest.

Changes in the intensity and impact of factors influencing consumer behaviour in the food market over timeOriginal Paper

Irena Anto¹ová, Jana Stávková

Agric. Econ. - Czech, 2023, 69(9):358-365 | DOI: 10.17221/191/2023-AGRICECON


The paper aims to identify the changes and intensity of the factors influencing consumers’ purchasing decision-making process in the food market. The research offers a unique comparison of the situation between the years 2007 and 2022 and reveals the emerging factors affecting consumer decisions. The authors conducted a representative questionnaire survey (N = 686) to determine the influence of individual factors. The data were processed using factor analysis, which allowed for the reduction of the number of factors (20) to four determinants covering 61% of the total variability in the dataset. The determinants of consumer purchasing decisions include traditional promotional elements, product parameters, newly formed factors based on sustainability and health awareness trends, and price discounts. Consumer behaviour appears to be influenced by a wide range of short- and long-term factors, and it is necessary to look at it comprehensively over a longer time horizon, taking current trends into account.

Proposal of a conceptual framework for creating a model for the implementation of green growth indicators in the conditions of the Slovak RepublicOriginal Paper

Samuel Fekiaè, Iveta Hajdúchová, Roman Dudík, Kim Hortenská, Martin Halász

J. For. Sci., 2025, 71(8):373-383 | DOI: 10.17221/22/2025-JFS

Green growth promotes sustainable economic development by protecting the environment and efficiently using natural resources. Supporting green growth enhances long-term sustainability, quality of life, and climate change mitigation. This article aims to propose a conceptual framework for implementing green growth indicators in Slovakia by comparing their perception with that of the Czech Republic. A questionnaire survey was conducted among 3 281 Slovak and Czech forestry enterprises [Nomenclature of Economic Activities (NACE) classification 02.10], with 1 365 responses. The findings highlight significant differences in the approach to green growth indicators between small and large enterprises and between state and non-state enterprises. Additionally, the study examines how Slovakia and the Czech Republic manage these indicators, forming the basis for a conceptual framework for their implementation in Slovakia. The research contributes to the discourse on green growth and sustainability, key concepts in the 21st century.

Balancing conservation and intervention: Managing forest diebacks in Slovakia's Tatra National ParkOriginal Paper

Paula Pu¹kárová, Mikulá¹ Èernota, Ján Slivinský

J. For. Sci., 2026, 72(3):119-135 | DOI: 10.17221/87/2025-JFS


Disturbance regimes in Central European mountain forests are changing under the ongoing climate change, raising questions about how protected areas should respond to increasingly frequent windthrow and bark beetle outbreaks. In Tatra National Park, a major windstorm in 2004 triggered a landscape-scale bark beetle eruption that challenged long-standing expectations that non-intervention alone could safeguard forest ecosystems. Using the Socio-Ecological Systems framework, we combined long-term field observations with archival sources and stakeholder interviews to examine how ecological dynamics, governance structures, and actor perspectives interacted during and after the outbreak. We show that warming, abundant post-storm breeding substrate, and legacy Norwegian spruce (Picea abies) monocultures reduced forest resilience and enabled successive beetle waves. Governance fragmentation led to delayed decision-making, allowing large volumes of wind-felled timber to remain untreated and facilitating its spread into high-naturalness stands. In contrast, evidence from other European countries suggests that timely, spatially focused measures can reduce outbreak duration without compromising conservation values. Our results indicate that in disturbance-prone, spruce-dominated forest systems experiencing climate change, a combination of strict protection in core areas and timely, selective measures in adjacent zones is more likely to maintain resilience and support conservation outcomes than strictly passive or fully interventionist approaches.

Hormetic growth of Pinus pseudostrobus seedlings exposed to low-dose gamma and X-ray irradiationOriginal Paper

Laura Yasmin Flores López, César Valenzuela Encinas, Eulogio De la Cruz Torres, María de Lourdes Palafox Chávez, Ivar Diego Aceves Sánchez, Lourdes Georgina Iglesias Andreu

J. For. Sci., 2026, 72(3):107-118 | DOI: 10.17221/83/2025-JFS

Mexico ranks among the countries with the highest deforestation rates, increasing the demand for high-quality forest seedlings of valuable species such as Pinus pseudostrobus Lindl., which face germination limitations due to seed dormancy and low viability. Ionising radiation has emerged as an alternative pregermination treatment capable of inducing adaptive responses in plants through hormesis. This study evaluated the effects of different doses of gamma radiation (60Co) and high-energy X-rays (linear accelerator, 6 MeV) on the germination, growth, and quality of P. pseudostrobus seedlings. A total of 1 440 seeds were irradiated per radiation source with 12 doses (0–25 Gy) and sown under nursery conditions in a completely randomised design. Germination parameters, morphological traits, photosynthetic pigment content, and quality indices were analysed. With both radiation sources, low doses (0.5–1.5 Gy) significantly enhanced germination, chlorophyll content, and seedling height and diameter, while doses above 15 Gy inhibited these responses. The LD50 (median lethal dose) was estimated at 20 Gy for gamma rays and 12 Gy for X-rays, whereas GR50 (median growth reduction dose) exceeded 45 Gy in both treatments. These findings demonstrate that low radiation doses elicit a beneficial hormetic effect in P. pseudostrobus, representing a viable biotechnological approach to improve seedling production and ecological reforestation efficiency.

Multivariate assessment of soil quality across different land use types in the hilly terrain of the subtropics of IndiaOriginal Paper

Jitendra Kumar, Homehswar Kalita, Rajesh A. Alone, Nishant K. Sinha, Alka Rani, Dhiraj Kumar, N. K. Lenka, Rajan Bhatt, Mohamed A. Yassin, Mohamed A. Mattar, Fülöp Attila, Ali Salem, Zol

Soil & Water Res., 2025, 20(3):178-194 | DOI: 10.17221/6/2025-SWR

The unsustainable conversion of forest areas into agricultural land poses a serious danger to the soil eminence of Arunachal Pradesh’s environmentally delicate hilly topography. Understanding the impacts of this land-use change is crucial for preventing further degradation. This study aimed to develop soil quality indices (SQIs) for different land use types: natural forest (NF), current jhum cultivation (JC), fallow jhum land (FJC), and pineapple cultivation (PA). Samples of soil were taken at a depth of 0 to 15 cm and examined for 22 potential soil quality indicators, with 19 showing significant (P < 0.05) influence from land use, constituting the total dataset (TDS). Principal component analysis (PCA) was employed on TDS to identify the minimum data set (MDS), comprising dehydrogenase activity, diethylenetriaminpentahacetic acid (DTPA)-extractable iron, and bulk density, contributing 73%, 19%, and 8% to the overall SQI, respectively. Subsequently, different SQIs were estimated using linear/nonlinear and additive/weighted scoring functions. The results revealed substantial alterations in SQIs among the land use types, through NF exhibiting the highest soil quality. Notably, the nonlinear SQIs exhibited greater sensitivity to land use conversion compared to their linear counterparts, indicating their potential as a more robust tool for assessing soil quality changes. This study concludes that the transformation of land use in the hilly regions of subtropics of Arunachal Pradesh has led to the deterioration of soil quality. The proposed indexing framework, leveraging the sensitivity and clarity of nonlinear SQIs, can effectively evaluate and compare soil quality across different land use scenarios, thereby informing sustainable land management strategies.

Does livestock manure recycling among acquaintance networks decouple crop and livestock production? Evidence from rural ChinaOriginal Paper

Ying Wang, Guanghui Jiang, Jisheng Min

Agric. Econ. - Czech, 2025, 71(9):512-525 | DOI: 10.17221/219/2024-AGRICECON

Livestock manure serves as a vital source of organic fertiliser, with efficient utilisation being crucial for sustainable agricultural development. However, with the development of specialised high-input agriculture, livestock manure recycling (LMR) is currently inefficient and leading to the imbalance between surplus manure and croplands. This study theoretically and empirically investigates whether LMR among acquaintance networks influence the crop–livestock integration (CLI) production. Based on survey data of swine farmers in rural China, the estimates indicate that LMR among acquaintance networks enhances the degree of CLI but may result in over-application of manure. Conversely, LMR with outside villagers or organisations is more likely to reduce the degree of CLI, possibly leading to under-application of manure. The motivation behind farmers' LMR plays a crucial role. Furthermore, the study indicates that formal institution can break the constraints of acquaintance networks on LMR and restructure the crop-livestock relationship beyond the household level. Our findings emphasise that LMR within rural China's acquaintance networks are in a transition phase, exhibiting both relational and market orientation characteristics. It is currently essential to maintain the balance between crop and livestock production, advance the development of LMR social services when designing LMR policies, and to leverage the complementary roles of informal and formal institutions.

Effect of microbial transglutaminase and banana peel powder on the structure and oxidative potentiality of camel milk yogurt during cold storageOriginal Paper

Rehab S. Alsulami, Elfadil E. Babiker, Isam A. Mohamed Ahmed, Tawfiq S. Alsulami, Fahad Y. Al-Juhaimi

Czech J. Food Sci., 2025, 43(5):365-375 | DOI: 10.17221/100/2025-CJFS

The current investigation was done to study the impact of adding banana peel powder (BPP) and treatment with microbial transglutaminase (MTGase) on the structure, physicochemical, antioxidant, and sensory qualities of yoghurt prepared from camel milk powder during cold storage. BPP analysis showed that it contained high amounts of total phenolics, which were accompanied by strong antioxidant activity. When employed alone or with BPP, MTGase polymerises yoghurt proteins, forming a structure with a high molecular weight above the stacking gel and lacking pores. The levels of protein, total phenolic compounds (TPC), total solids, ash, and antioxidant activity (DPPH – 2,2-diphenyl-1-picrylhydrazyl) increased after the addition of BPP to yoghurt, even after 21 days of storage. Adding BPP to yoghurt initially decreased thiobarbituric acid reactive substances (TBARS) levels, which then increased with the storage time. Yoghurt made with BPP and treated with MTGase was more stable with enhanced physicochemical, antioxidant, and sensory qualities during storage when compared to yoghurt made with MTGase alone. The results of the study demonstrated that MTGase is capable of cross-linking yoghurt proteins and that BPP can be applied as a stabiliser, antioxidant, and functional component in yoghurt production.

Endogenous inhibitory compounds in bovine milk, their significance and methods of detection

Marcela Klimesova, Ludmila Krizova, Hana Nejeschlebova, Oto Hanus, Lenka Vorlova

Czech J. Anim. Sci., 2025, 70(12):499-517 | DOI: 10.17221/146/2025-CJAS


Raw cow’s milk is one of the most strictly controlled commodities in the food industry. Besides basic hygienic and nutritional parameters, the presence of inhibitory and contaminant substances plays a critical role in technological quality and safety. While the vast majority of inhibitory residues are represented by antibiotics originating from mastitis prevention and therapy, milk also contains a broad spectrum of naturally occurring native antimicrobial compounds. These include immunoglobulins, lactoferrin, lysozyme, lactoperoxidase, phosphatases, lipases, and proteases acting as integral elements of the innate immune system within the mammary gland. Although these substances are not legislatively regulated, they significantly influence milk processing, starter culture growth, cheese yield, and shelf-life of final dairy products. Their concentration is strongly associated with animal health status, especially mastitis, and may thus also serve as an indirect quality indicator. This review provides a comprehensive overview of native inhibitory compounds in bovine milk, their concentration ranges, biological significance, impact on dairy technology, and the analytical methods currently used for their detection.

Beta-hydroxy-beta-methylbutyrate in functional food ingredients: An overview of biosynthesis, metabolic mechanisms and applicationsReview

Xizi Zhang, Huijing Zhang, Jiaxing Li, Chenchen Qi, Di Zhang, Wei Chen, Chengtao Wang

Czech J. Food Sci., 2026, 44(1):1-15 | DOI: 10.17221/88/2025-CJFS

β-Hydroxy-β-methylbutyrate (HMB) is a metabolite of the essential amino acid leucine, which can be produced naturally in mammals and is also found in trace amounts in citrus fruits and fish. Studies have shown that HMB plays an important role in maintaining human health by improving muscle health and inhibiting muscle catabolism. This review summarises the synthesis and metabolism of HMB and discusses its potential use as a nutrient, highlighting and analysing the importance of HMB supplementation for athletes' physical recovery and the treatment of muscular dystrophy-related diseases between 2019 and 2025. This study will help us to deepen our understanding of the application of HMB as a dietary supplement for the treatment of different diseases, providing the latest insights into its sustainability.

Performance and physiological responses of E. coli-challenged broiler chickens to dietary Moringa oleiferaOriginal Paper

Ari Hameed Omer, Sherzad Mustafa Hussein

Czech J. Anim. Sci., 2026, 71(2):79-93 | DOI: 10.17221/153/2025-CJAS


This study was conducted to examine the effects of Moringa oleifera (MO) seed powder on the performance and gut health of broilers challenged with E. coli. A total of 720 one-day-old Ross 308 broiler chicks were randomly allocated to 72 pens across two separate rooms, following a 2 × 6 factorial design. The factors per room included (i) challenge: no or yes, and (ii) feed additive: control (none), antibiotic [oxytetracycline (OTC) at 0.5 g/kg], MO 0.1%, MO 0.2%, MO 0.4%, and MO 0.8%. At 9 days old, birds in both challenged and non-challenged rooms were inoculated with 1.5 ml of E. coli-O157:H7 inoculant (3.8 × 108 CFU) and 1.5 ml of saline, respectively. Performance data showed that, throughout the study, unchallenged birds had better weight gain (WG) and feed conversion ratio (FCR) than challenged birds. On day 35, broilers given OTC and all MO levels showed improved WG (P < 0.001) and FCR (P < 0.003). Interactions between challenge and additives were observed on day 10 for FCR (P = 0.031); on days 24 and 35 for WG (P < 0.001); and on days 24 and 35 for FCR (P < 0.004 and P = 0.002, respectively). On day 24, challenged birds fed all MO levels exhibited higher WG and better FCR than challenged controls, while on day 35, both challenged and unchallenged MO-fed birds showed improved WG and FCR compared to control groups. E. coli significantly increased crypt depth (CD), jejunum muscle thickness, and caecal E. coli colonies. MO significantly enhanced villus height (VH), the VH : CD ratio, villous tip width, and surface area, while decreasing CD, muscle thickness, and E. coli colonies. Challenged birds had significantly lower serum total protein, albumin, and Newcastle Disease Virus (NDV) titres, while serum alanine aminotransferase ALT activity was higher than in non-challenged birds. Overall, Moringa at 0.4% showed comparable or better results than OTC in preventing E. coli-induced declines in broiler performance and gut health.

Degradation of Betula spp. under the influence of biotic factors in the forests of Ukrainian PolissiaOriginal Paper

Ivanna Kulbanska, Maryna Shvets, Svitlana Matkovska, Tetiana Melnyk, Vasyl Zayachuk, Nataliya Horbenko, Oleh Zymaroiev, Anastasiia Zymaroieva

J. For. Sci., 2026, 72(3):148-160 | DOI: 10.17221/96/2025-JFS


The paper examines the impact of biotic factors – mainly pests and pathogens – on the degradation of Betula spp. in the forests of Ukrainian Polissia. The taxonomic structure of the identified mycobiota includes representatives of 9 genera, 8 families, 5 orders, 4 classes, and two divisions (Ascomycota 44.4%, Basidiomycota 55.6%). Xylotrophic Basidiomycetes, particularly Fomitopsis betulina and Fomes fomentarius, pose the greatest threat due to their ability to destroy wood tissues. The bacterial pathogen Lelliottia nimipressuralis, the agent of wetwood, also plays a major role, impairing xylem water transport and causing systemic physiological imbalance in affected trees. Entomofauna significantly contributes to degradation processes. A total of 31 insect species were recorded, belonging to five orders, 24 families, and 29 genera, with Coleoptera being the most numerous (32.3%). The most harmful are xylophagous species (e.g. Agrilus betuleti, Rhagium mordax) and phytophagous species (e.g. Geometra papilionaria, Parornix betulae), which cause mechanical tissue damage and facilitate secondary infections. The results indicate that pathogenic complexes intensify under environmental stress, accelerating the decline of Betula spp. stands. These findings underscore the necessity for ongoing phytosanitary monitoring and adaptive management measures to mitigate further degradation risks in the birch forests of Polissia.

Soil macroarthropod dynamics in response to environmental disturbances in a forest remnant ecosystem: A case study at Cibodas Botanical GardenOriginal Paper

Anita Rianti, Fenky Marsandi, Taufikurrahman Nasution, Musyarofah Zuhri, Muhammad Efendi, Hari Prayogi, Setyawan Agung Danarto, Hidayatul Fajri, Vivin Silvaliandra Sihombing (ORC

J. For. Sci., 2026, 72(1):1-13 | DOI: 10.17221/38/2025-JFS


Disturbing the remaining forest ecosystem in the Cibodas Botanical Garden (CBG) has affected the dynamics of the soil macroarthropod communities. This study was conducted in three remaining forest locations in the CBG with different levels of disturbance. Soil macroarthropod samples were collected using the pitfall trap method with 30 traps and analysed using the Shannon-Wiener diversity index, Pielou's evenness, Simpson's dominance, and Margalef's species richness to assess the dynamics of the soil macroarthropod community. This study analysed how these communities respond to different levels of disturbance in the garden, namely Jalan Akar (JA; low), Wornojiwo (WJ; moderate), and Ciismun (CI; high), which were influenced by tourism activities and local environmental conditions. The results showed that individuals from the Hymenoptera group accounted for 60.05% of the total number of soil macroarthropods found. Site WJ, which experienced moderate disturbance, had the highest number of individuals and species richness of soil macroarthropods. In contrast, site CI, which experienced high levels of disturbance, had a lower number of individuals and lower species richness, diversity and evenness indices. Site JA, which experienced low levels of disturbance, exhibited higher diversity and evenness indices. These results demonstrate that disturbance affects the presence of soil macroarthropods at their respective levels of disturbance. However, analysing the spatial distribution of soil macroarthropods in each studied taxon using the Morisita index revealed that they were dominantly clustered and exhibited varied distribution patterns. The study concludes that maintaining minimal disturbance is essential to preserve soil biodiversity and ecological balance in managed forest ecosystems such as the Cibodas Botanical Garden.

Coppice forests: Between management, conversion and restorationReview

Valeriu-Norocel Nicolescu, Gavriil Spyroglou, Sanja Periæ, Martina Ðodan, Cornelia Buzatu-Goanțã, Milun Krstic, Joao Carvalho, Iryna Matsiakh, Halil Bariº Özel (ORCID:

J. For. Sci., 2026, 72(2):57-72 | DOI: 10.17221/5/2026-JFS


Coppice is the oldest form of systematic and sustainable use of forests, and is currently applied on about 29 million ha (about 14% of total forest land area) in Europe. It had its maximum spread in the 16th century, when an estimated 36% of all forested land in Europe was managed for coppice. Coppice forests were the most important source of fuelwood until the mid-19th century, when firewood and charcoal were substituted by alternative fuels, and the demand for construction wood increased. Consequently, coppices (both low and coppice-with-standards) started to be converted to high forests, and the process was driven by national policies and/or subsidies, which is still the situation in some European countries. During the 20th century, coppicing was abandoned in many places across Europe due to the abandonment of the countryside and population migration into cities, as well as changes in socio-economic conditions, technical advances and political restrictions. However, coppicing is still important in many European countries as the main source of firewood for the rural population, who has limited access to other sources of energy. In this context, this paper presents the most important characteristics of this complex abandonment/management/conversion picture, emphasising the pros and cons for the future of coppices across Europe.

Evapotranspiration and crop coefficient of drip irrigated walnut trees in semi-arid climatic conditions, TürkiyeOriginal Paper

Tolga Erdem, Erhan Göçmen, Mehmet ªener, Zafer Çoºkun, Ahmet Semih Yaºasın, Buse Önler

[Ahead of Print]CAAS Agricultural Journals, X:X | DOI: 10.17221/72/2025-SWR


This study was conducted to find out the seasonal evapotranspiration (ETc) and crop coefficient (Kc) for walnut trees (ages one to nine) that were grown with drip irrigation in Türkiye’s semi-arid climate. Three different irrigation levels were applied at five-day intervals based on cumulative Class A pan evaporation using irrigation treatment coefficients (Kt = 0.75, 1.00, and 1.25) during the 2015, 2016, 2017, 2018, 2019, 2021, 2022, and 2023 growing seasons. The amount of irrigation water applied to the treatments varied from year to year according to the measured Class A pan evaporation amounts. The total amount of irrigation water applied to the treatment subjects varied between 371.7 mm and 619.6 mm as an average of eight years. Total ETc of walnut trees varied over the years depending on the applied irrigation water and measured rainfall. The total evapotranspiration estimated from the I2 treatment, representing the irrigation regime in which 100% of Class A pan evaporation was applied, fluctuated between 676.5 and 585.9 mm over the study years. The daily reference evapotranspiration (ET0) values are calculated as between 1.85 and 7.07 mm/day. The Kc values for walnut trees were calculated as 0.55 for April, 0.71 for May, 1.02 for June, 1.07 for July, 1.01 for August, and 0.74 for September on average. The research revealed that seasonal evapotranspiration and plant coefficient values can assist in calculating the water requirements of walnut trees and improve water management in semi-arid regions.

Assessing the impact of China's National Big Data Comprehensive Pilot Zone policy on agricultural carbon emissionsOriginal Paper

Yuqian Zhang, Yixuan Wang, Chenchen Su, Jiahong Guo, Chen Xu

Agric. Econ. - Czech, 2025, 71(5):242-253 | DOI: 10.17221/205/2024-AGRICECON


The global focus on the relationship between digitisation and agricultural carbon emissions remains high. However, research on the systemic ramifications of comprehensive digital policy implementation remains limited. Against the backdrop of China's pursuit of carbon neutrality and carbon emission peaking targets, we employed the difference-in-differences method to investigate the impact of applying a digital policy on agricultural carbon emissions. Our findings indicated that the implementation of the National Big Data Comprehensive Pilot Zone policy could effectively mitigate agricultural carbon emissions, resulting in a sustained positive influence. The intermediary mechanism test results validated the beneficial effects of financial expenditures on science and technology, as well as the number of information practitioners. The regional heterogeneity analysis results revealed that the policy effect was obvious in the major grain-producing areas but not in the major grain-selling areas or production–marketing balance areas. Additionally, differences in policy effectiveness were observed across different crop types. This study not only offers valuable insights for agricultural carbon reduction in China but also provides robust case data and guidance for other developing countries worldwide in the formulation and execution of digital policies aimed at promoting agricultural carbon emission reduction.

Regional patterns and cluster analysis of agricultural methane emissions in the EU-27 countriesOriginal Paper

Klára Bartoòová, Jan Saro, Mansoor Maitah, Jaromír Ducháèek

Czech J. Anim. Sci., 2025, 70(7):253-266 | DOI: 10.17221/26/2025-CJAS


Agricultural methane emissions strongly contribute to global greenhouse gas production. Under these circumstances, meeting international climate goals, including the Global Methane Pledge or the European Green Deal, requires developing targeted mitigation strategies. However, research using advanced clustering techniques in a multilevel context remains scarce and mostly limited to CO2 emissions. This lack of time-series studies addressing regional variability hinders efforts to develop effective mitigation strategies. This study addresses three main research questions: (i) What are the main trends in agricultural methane emissions in the EU-27 countries from 2013 to 2022? (ii) How can the EU countries be classified based on agricultural methane emissions per capita? (iii) What is the impact of selected agricultural and economic indicators, including the number of live bovine animals and land use, on the clustering of methane emissions? Combining hierarchical and k-means clustering with trend analysis, this research integrates data from Eurostat and the World Bank, thereby classifying the EU-27 countries into four clusters based on their agricultural practices and methane emissions profiles. The results highlight distinct emission patterns across the EU-27 regions, with farming systems characterised by high stocking rates and intensive production generating the highest per capita emissions. By contrast, extensive systems with lower animal density exhibit reduced methane intensities. These findings underscore the need to devise effective, region-specific, data-driven policies and strategies for mitigating methane emissions.

Can sustainable practices optimise fertiliser use and economic efficiency? A micro-panel analysisOriginal Paper

Faruque As Sunny, Juping Lan, Mohammad Ariful Islam

Agric. Econ. - Czech, 2026, 72(1):1-18 | DOI: 10.17221/473/2024-AGRICECON

The intensification of agricultural practices in Bangladesh has caused significant environmental challenges. This has also undermined farmers' economic sustainability, mainly due to the excessive use of subsidised chemical fertilisers. To address these issues and align with the United Nations Sustainable Development Goals (SDGs), Bangladesh has prioritised the adoption of sustainable farming practices, including the recommended fertiliser application (RFA). However, whether the adoption of RFA ensures economic sustainability remains uncertain. This study evaluates how the Bangladesh Rice Research Institute's (BRRI) proposed RFA affects fertiliser use and cost-efficiency. Drawing on five years (2017–2021) of panel data from 2 025 households across three acidic soil regions in Dinajpur, the findings reveal that RFA adoption reduces fertiliser use by 12% while improving cost efficiency by 4.9–5.1%. These results highlight the potential of RFA to mitigate environmental degradation while enhancing economic outcomes, thereby supporting the SDG agenda. In light of these benefits, the study offers key insights for policymakers and development practitioners, emphasising the need for targeted interventions to accelerate RFA adoption and promote sustainable agriculture.

On the origin of Bohemian cattle according to mitochondrial DNA

Karel Novák, Vojtìch Janák, René Kyselý

Czech J. Anim. Sci., 2026, 71(3):118-128 | DOI: 10.17221/127/2025-CJAS

The genomic structure of extant cattle populations can contribute to the reconstruction of the history of particular breeds or their subpopulations. Genome-wide population resequencing of extant populations of Czech Red Pied (CRP) cattle, its conserved nucleus herd, and Czech Red (CR) cattle detected a T106C polymorphism in mitochondrial DNA shared by the conserved CRP herd with a geographical belt of Anatolian, Illyrian and Eastern Alpine breeds. On the other hand, this SNP is practically absent in the historical cattle breeds associated with Northern Germany, including German Black Pied cattle, Holstein-Friesian and German Red Mountain cattle (GRM). Correspondingly, this indicator SNP was absent in CR cattle, which, like the GRM, belongs to the group of mountain red cattle breeds. It seems that the precursory cattle population in Central Europe was influenced by the germplasm from the Balkans-related group of breeds, thanks to the political and commercial influence of the Northern Italian, Austrian and Hungarian regions. In addition, the presence of this polymorphism in some European aurochs (Bos primigenius) bone remains suggests possible introgression from local aurochs populations. Alternatively, the T106C presence in yaks and in neighbouring cattle breeds in Northern India, China and Korea raises the possibility of origin of this polymorphism from yak populations. The spread of the T106C mutation in alpine regions is consistent with the known role of this mtDNA region in adaptation to the reduced oxygen pressure.

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