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Results 901 to 930 of 1498:

Sixty years of the Crop Research Institute and its impact on plant breedingScientific Reports

I.T. Prášil, J. Chrpová, Z. Stehno

Czech J. Genet. Plant Breed., 2011, 47(4):172-180 | DOI: 10.17221/174/2011-CJGPB

Critical success factors influencing the transformation of the agricultural biotechnology industry in Taiwan

Meng-Shiunn LEE

Agric. Econ. - Czech, 2012, 58(6):249-263 | DOI: 10.17221/60/2011-AGRICECON

The study uses the fuzzy Delphi and FANP (fuzzy analysis network process) methods to integrate the views of experts from enterprises and academia regarding the future direction of the agricultural biotechnology industry in Taiwan, in order to extract the critical success factors influencing its transformation. This study compares the differences in the "cross-domain" and "non-cross-domain" critical success factors, and expects that the research results could be a decision-making reference for both the industry and the government. The research results show that the cross-domain critical factor that influence the transformation of the agricultural biotechnology industry in Taiwan are sequentially: a stable source of raw materials, control of the trend of market demand, a new production equipment, the capability in supporting upstream and downstream industries, the integration of the human resources of the upstream, midstream, and downstream industries, and the capability in marketing and promotion. The non-cross-domain critical factors are sequentially: the capacity to establish the system of product technology, the capacity to control intellectual property rights, a perfect educational system, the capacity to carry out risk management of the new product development, the competence in cultivating the capacity of technical and R&D personnel, and laws and regulations.

How does general economy and agriculture sector performance influence farm producer support in the OECD countries?

Tomasz Siudek, Aldona Zawojska

Agric. Econ. - Czech, 2012, 58(3):101-118 | DOI: 10.17221/57/2011-AGRICECON

This paper assesses the effects of the performance of the OECD national economies and agricultural sectors on the farm producer support for the years 1986 to 2009. The study is complementary to the large amount of microeconomic research that highlights the importance of support to agricultural industry. Data for the analysis are taken from the OECD Producer and Consumer Support Estimates database and the World Bank World Development Indicators 2010 database. The Producer Support Estimate - PSE (expressed in absolute value, in percent of the total farm revenues and per 1 hectare of agricultural land) was taken as the dependent variable, whereas the selected indicators describing the performance of the economies (e.g. GDP per capita, unemployment, fiscal balance, government debt, government expense and tax revenue, exchange rate, agriculture share in GDP and employment, agricultural raw materials exports and imports) were the independent variables. Utilizing these variables, a simple linear regression analysis was conducted and resulted in many significant associations. In the period analyzed, there was a wide gap between the most and least farm supporting countries in terms of the annual average percentage PSE and the PSE per hectare. Substantial differences between the countries in the variability of the PSE over the time occurred. The empirical results obtained from the regression models reveal, among other, that when the countries were becoming richer, the percentage PSE was generally decreasing. Mixed results were obtained for the relationship between the percentage PSE and unemployment, as in some countries it was negative, while in the others positive. Expansionary fiscal policy exerted opposite effects on the PSE in different countries. Labour productivity in agriculture was inversely correlated with the percentage PSE.

Optimal inclusion level of butylated hydroxytoluene in semen extender improves the quality of post-thawed canine sperm

M. Ziaullah, A. Ijaz, M. Aleem, A.K. Mahmood, H. Rehman, S. Bhatti, U. Farooq, M.U. Sohail

Czech J. Anim. Sci., 2012, 57(8):377-381 | DOI: 10.17221/6273-CJAS

The study was conducted to evaluate the potential cryoprotective effect of butylated hydroxytoluene (BHT) through post-thaw evaluation of canine semen and its optimal inclusion level. Ejaculated canine semen was extended in TRIS-glucose egg yolk extender containing various concentrations of BHT (0.5, 1.0, 1.5, 2.0, and 2.5mM). Semen was frozen at -196°C using 200 × 106 spermatozoa per 0.5 ml straws and post-thaw evaluation was carried out in terms of sperm motility, viability, plasma membrane integrity, and acrosomal integrity through phase-contrast microscope, supravital staining, hypo-osmotic swelling test, and normal acrosomal ridge, respectively. BHT was found to improve (P > 0.005) all post-thawed semen quality parameters at an inclusion level of 1.0mM in the extended semen. However, higher concentrations than this were found to have detrimental effects.

Factors affecting the development of Phytophthora alni ssp. alni infections in Alnus glutinosa L.

P. Štochlová, K. Novotná, K. Černý

J. For. Sci., 2012, 58(3):123-130 | DOI: 10.17221/26/2011-JFS

Phytophthora alni is responsible for a devastating disease in alder and changes in the environment of riparian and alder carr ecosystems. One of the main approaches to solve this problem is to find naturally resistant genotypes using a series of artificial inoculation experiments, to preserve and use them in programmes for resistance breeding. However, the results of artificial inoculation experiments (screening for natural resistance) can be affected by several factors. The potential effect of the social status of the host, the presence of naturally occurring P. alni infections, the season and the size of the sections of branches used were studied in a series of infection experiments. It was found out that the development of lesions was significantly affected by the year season (the largest lesions were found in summer) and by the presence of naturally occurring P. alni infections in the sampled trees (the lesions were five times larger in healthy trees and trees recovered from natural P. alni infections compared to trees with active disease development).

Prediction of crude protein content in rice grain with canopy spectral reflectance

H. Zhang, T.Q. Song, K.L. Wang, G.X. Wang, H. Hu, F.P. Zeng

Plant Soil Environ., 2012, 58(11):514-520 | DOI: 10.17221/526/2012-PSE

Non-destructive and rapid monitoring methods for crude protein content (CPC) in rice grain are of significance in nitrogen diagnosis and grain quality monitoring, and in enhancing nutritional management and use efficiency. In this study, CPC and canopy spectra in rice were measured based on rice field experiment. Key spectral bands were selected by principal component analysis (PCA) method, and the predicted models were built by multiple linear regressions (MLR), artificial neural network (ANN) and partial least squares regression (PLSR). The results showed that there is a significant correlation between CPC content and key spectral bands. The results of prediction for the three models were in order of PLSR > ANN > MLR with correlation values of 0.96, 0.92 and 0.90, respectively, for the validation data. Therefore, it is implied that CPC in rice (grain quality) could be estimated by canopy spectral data.

Prevalence, antimicrobial resistance, and molecular characterization of Campylobacter jejuni and C. coli isolated from retail raw meat in Poland

K. Wieczorek, R. Szewczyk, J. Osek

Vet Med - Czech, 2012, 57(6):293-299 | DOI: 10.17221/6016-VETMED

The study was conducted to investigate the presence of Campylobacter spp. in meat sold to consumers at a retail market in Poland. Antimicrobial resistance and the presence of putative virulence genes of the isolates were also examined. A total of 558 meat samples, including beef (n = 105), pork (n = 85), and poultry (n = 368) were collected over an almost three year study period. It was found that 321 samples, all of them originating from poultry, were contaminated with Campylobacter spp. Most of the obtained isolates were classified as C. coli (189 strains, 58.9%), whereas C. jejuni was identified in 132 (41.1%) samples. All Campylobacter strains were susceptible to gentamicin and all but one C. coli isolate to erythromycin. On the other hand, the highest level of resistance among Campylobacter tested was to ciprofloxacin (91% for C. jejuni and 86.1% for C. coli) and nalidixic acid (89.3% for C. jejuni and 85% for C. coli). Furthermore, resistance to two or more classes of antibiotics was found in the majority (60.9%) of Campylobacter spp. and among them one C. coli strain showed resistance to four different classes of antimicrobials. Identification of virulence genes in the isolated Campylobacter showed that all of them had the flaA and cadF genes. The iam marker was found more often in C. coli strains (88.8%) compared to C. jejuni isolates (53.8%). On the other hand, the virB11 gene was identified only in 4.2% of C. coli and in 6.1% of C. jejuni strains, respectively. Furthermore, the prevalence of the cdtA, cdtB, and cdtC genes among C. jejuni strains was 97.7%, 93.2%, 96.2%, respectively, and was significantly higher than for C. coli regarding the cdtC (66.7%) gene. The obtained results showed that the presence of Campylobacter in retail meat may represent a threat to public health.

Mercury speciation and safety of fish from important fishing locations in the Czech Republic

Renáta KENŠOVÁ, Kamila KRUŽÍKOVÁ, Zdeňka SVOBODOVÁ

Czech J. Food Sci., 2012, 30(3):276-284 | DOI: 10.17221/239/2011-CJFS

The aim of the study was to describe the distribution of total mercury in the tissues of fish originating from important Czech fishing locations and to determine the level of methylmercury as a percentage of total mercury (Hg speciation). At six important fishing locations a total of 144 fishes of 13 species were caught and analysed. Samples of muscle, liver, gonads and scales were analysed for total mercury and in the case of muscles for methylmercury concentrations. Total mercury was determined by AAS using an AMA 254 analyser, and methylmercury determination was performed by GC/ECD using a GC-2010A chromatograph. Total mercury concentrations in muscle and other tissues of all the fish from all fishing locations were below 0.5 mg/kg, with the exception of 6 asps (Aspius aspius). A significant (P < 0.05) correlation was found between the total mercury concentrations in scales and other tissues. Methylmercury made up about 46-100% of total mercury in muscle. The overall results confirmed the good hygienic quality of fish from important Czech fishing locations.

Evaluating productivity, cost, chip quality and biomass recovery for a mobile chipper in Australian roadside chipping operations

M.R. Ghaffariyan, J. Sessions, M. Brown

J. For. Sci., 2012, 58(12):530-535 | DOI: 10.17221/51/2012-JFS

The Bruks mobile chipper was tested for chipping extracted non-merchantable stemwood at the roadside in Pine plantation in Victoria. The elemental time study method was used to evaluate the system productivity. The productivity, cost, biomass yield, remaining slash, chip quality (size classification and energy content), and fuel and energy consumption were analysed. Chipping extracted small logs at the roadside yielded a productivity of 43.88 GMt.PMH0-1 (19.4 BDT.PMH0-1). The average cost was about 16.96 USD.GMt-1 (38.36 USD.BDT-1).

Ovarian follicle growth dynamics during the postpartum period in Holstein cows and effects of contemporary cyst occurrence

R. Rajmon, J. Šichtař, L. Vostrý, D. Řehák

Czech J. Anim. Sci., 2012, 57(12):562-572 | DOI: 10.17221/6414-CJAS

The indicators of follicle development with regard to the growth wave order, the first ovulation, animal parity, and also with regard to the simultaneous presence or absence of a follicular cyst were determined in cows in the course of 60 days postpartum. Follicular dynamics were monitored daily by ultrasonography. The animals were assigned to three groups based on the time of the 1st ovulation: G1 (n = 9) - the 1st dominant follicle (DF) ovulated, G2 (n = 10) - ovulation occurred on the 2nd or later follicular waves, and G3 (n = 5) - no ovulation occurred during the experimental period. G1 animals showed better fertility later (no cyst, less days open, P = 0.07, less hormonal treatment, P = 0.008). The rhythm of follicular wave development was generally similar in all the animals (based on emergence of the first follicular wave, the interval from emergence to deviation, and the number of all follicular waves). Nevertheless, emergence of follicular waves and deviation occurred by 0.5-0.9 day earlier in primiparous than in multiparous cows and in G1 vs. G2, or G3, respectively (in all P < 0.05). DF development was independent of parity as well as group effects, but the maximum size and growth rate (1.2 vs. 0.8 cm/day, P < 0.05) were higher in ovulatory follicles (OF) than in regressive ones (rDF). The presence of a growing cyst decreased the probability of rDF as well as OF development (P < 0.0001). The OF growth rate was faster in the milieu of a stagnating cyst than without any cyst (P < 0.04). Therefore, the development of follicles was dramatically suppressed beyond, but nor before, deviation in the milieu of a growing cyst. Cessation of the cyst growth accelerated the development of OFs. On the contrary, a cystic structure without any significant growth can persist for weeks with no effect on successful follicular development.

Legal framework for payments for forest ecosystem services in the Czech Republic

K. Ventrubová, P. Dvořák

J. For. Sci., 2012, 58(3):131-136 | DOI: 10.17221/71/2011-JFS

Although examples of payments for ecosystem services (PES) can be traced back at least as far as the 1980's, it is still a relatively new instrument, and the hitherto experience in many parts of the world is not extensive yet, or based on a very long timeframe. In addition, PES is being introduced in more and more sectors (agriculture, water supply, carbon sequestration, biodiversity conservation, etc.) and in relation to more and more ecosystems (surface water, groundwater, forest, etc.). But such PES can work only with good governance in place, comprising an effective political, legislative as well as institutional system. Nevertheless, an effective introduction of PES system into national legislation calls for an appropriate analysis of the current legal system so that duplications can be excluded and only valuable measures can be added. The main goal of this paper is to analyze if the current legal framework of the Czech Republic provides an appropriate basis for establishing payments for a forest ecosystem services scheme.

List of field crop varieties registered in the Czech Republic in 2011New Varieties

R. Šafaříková

Czech J. Genet. Plant Breed., 2012, 48(1):48-49 | DOI: 10.17221/52/2012-CJGPB

Effect of storage temperature on the quality of dry fermented sausage Poličan

Josef Kameník, Alena Saláková, Gabriela Bořilová, Zdeněk Pavlík, Eva Standarová, Ladislav Steinhauser

Czech J. Food Sci., 2012, 30(4):293-301 | DOI: 10.17221/284/2011-CJFS

The influence of different storage temperatures (5°C and 15°C) on the quality of vacuum-packed dry fermented sausage Poličan was determined. The salami mixture, finished salamis (the maturing period of 30 days), and salamis stored for 30, 60, 90, and 120 days were analysed. The analyses performed (physical/chemical, sensory, microbiological) found no differences in sensory properties or basic physical/chemical and microbiological parameters in the products after storage under different temperature conditions for 120 days. When stored at 15°C, the total content of biogenic amines in samples was higher than that for samples stored at 5°C with statistical differences P ≤ 0.05. If the principles of good manufacturing practice are observed at all phases of the technological process, the storage temperature of 15°C does not represent a risk as the consequent concentration of biogenic amines and polyamines remains extremely low.

Characterization of M. laxa and M. fructigena isolates from Hungary with MP-PCR

Sz. Sződi, H. Komjáti, Gy. Turóczi

Hort. Sci. (Prague), 2012, 39(3):116-122 | DOI: 10.17221/216/2011-HORTSCI

Monilinia laxa (Monilia laxa), Monilinia fructicola (Monilia fructicola) and Monilinia fructigena (Monilia fructigena) are the causal agents of brown rot on pome and stone fruits in Hungary. Forty-five isolates collected from different hosts, different years in several orchards were used for characterization of the M. laxa and M. fructigena population in Hungary. The isolates were identified on species level based on morphological and molecular biological methods; out of these 24 were M. laxa, 20 were M. fructigena and 1 was M. fructicola. Populations of the three Monilinia species were studied with microsatellite primers and the degree of genetic diversity within the species was measured. The population structure analysis revealed that genetic diversity within M. laxa subpopulations was HS= 0.1599, while within M. fructigena subpopulations was HS= 0.2551. The total genetic diversity was HT= 0.3846, while genetic diversity between M. laxa and M. fructigena subpopulations was DST= 0.1771. No clustering relationship was observed among isolates by the different years or hosts.

Impacts of the US Farm Bill 2008 on China's agricultural production and rural poverty

Hai Lin, Thomas Glauben, Jun Yang, Ling-Yun He

Agric. Econ. - Czech, 2012, 58(4):157-164 | DOI: 10.17221/122/2011-AGRICECON

Whether the US Farm Bill 2008 has any impacts on China's agriculture, is highly related to the China's rural poverty alleviation and it deserves to be explored to see what will happen. According the existing empirical studies in the past, we expect that the US Farm Bill 2008 will exert an influence on the agricultural production and rural poverty in developing countries, especially China. In order to fully capture such an effect, we link two equilibrium models together: the multinational CGE model - GTAP and the China's partial equilibrium model - the China's Agricultural Policy Simulation and Projection Model (CAPSiM). A scenario based on the US Farm Bill 2008 will be constructed and compared with the baseline. A more liberalized scenario is also built for the simulation. The results have shown that the US Farm Bill 2008 has a little positive impact on the China's farmer income, and that the more liberalized the policy is, the more China will benefit.

Transient ileocolic intussusception in a dog with histiocytic ulcerative tiflocolitis: a case report

M. Garcia-Sancho, F. Rodriguez-Franco, A. Rodriguez-Bertos, V. Carrasco, A. Villaescusa, A. Sainz

Vet Med - Czech, 2012, 57(8):434-438 | DOI: 10.17221/6312-VETMED

In human medicine, the association between intussusceptions and inflammatory bowel disease is well known, even if referred to only in case reports or a small series of patients. The exact cause of intussusceptions, in general, is unknown; however, any lesion in the bowel wall or irritant in the lumen that alters the normal peristaltic pattern may initiate invagination. In Crohn's disease the lead point may be dysrhytmic contractions secondary to the on-going inflammatory process of a thickened, inflamed segment of bowel wall. In veterinary medicine, the information available related to the association of intussusceptions and inflammatory bowel disease is limited. The present study is the first reported case of a dog in which ileocolic intussusception and histiocytic ulcerative colitis appear to be associated. Moreover, this is also the first description of the involvement of the caecum in canine histiocytic ulcerative colitis. Herein, we document a condition previously reported in humans with inflammatory bowel disease, and we believe that this work contributes to identifying the similarities between human and canine inflammatory bowel disease.

Pseudo-embryogenic structures in anther and isolated microspore cultures in vitro: a cautionary guideReview

Ugur BAL, Mehran E. SHARIATPANAHI, Antonio J. CASTRO, Delphine EMERY, Christophe CLÉMENT, Maryam DEHESTANI-ARDAKANI, Khadijeh MOZAFFARI, Alisher TOURAEV

Czech J. Genet. Plant Breed., 2012, 48(2):51-60 | DOI: 10.17221/176/2011-CJGPB

This review describes sources of structures of non-microspore origin observed in anther and microspore cultures. Various characteristics of these structures may cause a wrong diagnosis of these structures as embryos or cell/tissue clusters of microspore origin. Here we suggest such structures to be named as pseudo-embryogenic structures. The introduction of pseudo-embryogenic structures and their origins could be helpful to distinguish them from true microspore-derived structures. Prompted by certain environmental cues, somatic cells existing as a contamination in immature pollen (microspores) cultures can lead to the formation of 'pseudo-embryos' commonly known as embryoids. The pseudo-embryogenic structures may be classified in the following groups: (i) pseudo-star-like structures; pseudo-multicellular structures; (ii) pseudo-embryos with pseudo-suspensors; (iii) contaminating bacteria appearing as callus colonies; (iv) calli and embryos of somatic origin; (v) giant tetrad-like structures; (vi) anther wall cells. The exact origin of these structures is discussed in this paper, and some recommendations are proposed in order to avoid misinterpretation.

Effect of dose of thymol and supplemental flavours or camphor on palatability in a choice feedings study with piglets

J. Michiels, J. Missotten, A. Ovyn, N. Dierick, D. Fremaut, S. De Smet

Czech J. Anim. Sci., 2012, 57(2):65-74 | DOI: 10.17221/5133-CJAS

Thymol's antimicrobial properties urged researchers to study its effect on animal performance and intestinal health in pigs. However, thymol has the characteristic sharp odor of thyme and a bitter, aromatic, and sometimes burning sensation which may elicit feeding aversions. The objectives in the current study were: (1) to determine the effect of dose of thymol and camphor on palatability and (2) to test the hypothesis that supplemental flavours or camphor, the latter as a known Transient Receptor Potential A1 blocker, could mitigate feed avoidance caused by thymol. Two analogous choice-feeding trials were conducted. Feed intake of the test diet was expressed as proportion of the total intake and tested by means of a one-sample Student's t-test against a set value of 50%. The preference for feed supplemented with 125, 500, 1250 and 2000 mg/kg thymol was 53.7 ± 6.0% (P > 0.05), 47.5 ± 5.1% (P > 0.05), 36.8 ± 4.9% (P = 0.022), and 3.9 ± 7.9% (P = 0.005) respectively. When feed containing 2000 mg/kg thymol with either flavour A (containing intense sweeteners) or flavour B (containing the same intense sweeteners and a caramel aroma) was opposed against a control diet, the relative intake of the test diets was 19.9 ± 5.8% and 14.0 ± 4.9% (both P < 0.05) respectively. When animals were offered one of these test diets and a reference diet with 2000 mg/kg thymol, animals exhibited a preference for the feed with 2000 mg/kg thymol + flavour A, but not for the feed with 2000 mg/kg thymol + flavour B. Thus, supplemental flavours containing intense sweeteners partially overcame feed avoidance caused by thymol which was less pronounced when the caramel aroma was present. Exposure to camphor (50 and 200 mg/kg) did not improve feed preference for a diet containing 1250 mg/kg thymol. Thymol's bitter taste might be largely responsible for the recorded feed refusal at high inclusion rates.

Simulation of soil organic carbon changes in Slovak arable land and their environmental aspectsOriginal Paper

Gabriela Barančíková, Jarmila Makovníková, Rastislav Skalský, Zuzana Tarasovičová, Martina Nováková, Ján Halás, Monika Gutteková, Štefan Koco

Soil & Water Res., 2012, 7(2):45-51 | DOI: 10.17221/38/2011-SWR

One of the key goals of the Thematic Strategy for Soil Protection is to maintain and improve soil organic carbon (SOC) stocks. A decline of SOC stocks is politically perceived as a serious threat to soil quality and functions. A suitable tool for acquiring the information on SOC stock changes is modelling. The RothC-26.3 model was applied for long-term modelling (1970-2007) of the SOC stock in the topsoil of croplands of Slovakia. Simulation results show a gradual increase in the SOC stock in the first phase of modelling (1970-1995) mainly due to higher carbon input in the soil. A significant linear correlation (r = 0.4**, n = 275) was found between carbon input and the final simulation of SOC stock. A close relationship between the SOC stock and soil production potential index representing the official basis for soil quality assessment in Slovakia was also determined and a polynomial relationship was found which describes the relation at the 95% confidence level. We have concluded from the results that balanced or positive changes in the SOC stock dynamics that are important for sustainable use of soils could be influenced positively or negatively in Slovakia by political decisions concerning the soil management. Moreover, the soil production potential index can be used as soil quality information support for such decision-making.

Modelling the factor content of agricultural trade

Ján POKRIVČÁK, Pavel CIAIAN, d'Artis KANCS

Agric. Econ. - Czech, 2011, 57(8):370-383 | DOI: 10.17221/116/2010-AGRICECON

This article examines the factor content of agricultural trade in the Central and East European (CEE) transition countries. It relates the factor content of agricultural trade to the cross-country differences in technology stemming from different farm organisations between the CEE countries agricultural sectors and to the differences in relative factor endowments. The relative factor endowments alone do not satisfactorily explain agricultural trade flows in the CEE countries. We find that transaction costs and market imperfections that affect the organisation of production also distort farm specialisation and hence V the actor content of agricultural trade.

Effect of thickening agents on perceived viscosity and acidity of model beverages

Zdenka PANOVSKÁ, Alena VÁCHOVÁ, Jan POKORNÝ

Czech J. Food Sci., 2012, 30(5):442-445 | DOI: 10.17221/286/2011-CJFS

The effect of thickening agents - methyl cellulose, hydroxyethyl cellulose, sodium carboxymethyl cellulose, and xanthan gum - solutions on the sensory viscosity was investigated in the concentration range of 0-0.8%. The perceived viscosity was proportional to the logarithm of kinematic viscosity in the presence of citric and malic acids. The viscosity was inversely proportional to the acidity at the viscosity levels higher than 10 mm2/s. A liquid of high viscosity thus possess lower acidity than aqueous or low-viscosity solutions. No significant differences were found between the effects of different thickening agents.

Tracking of powdery mildew and leaf rust resistance genes in Triticum boeoticum and T. urartu, wild relatives of common wheatOriginal Paper

Nelli A. Hovhannisyan, Mohammad Ehsan Dulloo, Aleksandr H. Yesayan, Helmut Knüpffer, Ahmed Amri

Czech J. Genet. Plant Breed., 2011, 47(2):45-57 | DOI: 10.17221/127/2010-CJGPB

Wild Triticum and Aegilops species are increasingly used in wheat breeding programmes around the world as donors of genes conferring resistance to biotic and abiotic stresses, as well as of genes that contribute to the improvement of grain quality. In the present study, thirty-nine accessions of diploid species with the A genome (Triticum boeoticum and T. urartu) were evaluated for the presence of the genes conferring resistance to powdery mildew (Blumeria graminis) and leaf rust (Puccinia recondita) using both inoculation tests and sequence tagged sites (STS) marker analyses in order to find correspondence between STS markers and resistance as a trait. The most resistant entries were T. boeoticum accessions. All the marked Lr and Pm resistance genes (Pm1, Pm2, Pm3, Lr10, Lr47, Lr25 and Lr28) were identified in the check T. aestivum cultivar Bezostaya 1. The resistance to powdery mildew in the material studied was conferred by the combination of the Pm1 gene with either Pm2 or Pm3. The Pm1 and Pm3 markers appeared to be suitable for tracking these powdery mildew resistance genes, while the Pm2 gene marker cannot be considered as usable in various genetically different wheat accessions. The presence of the genes Lr25, Lr28 and Lr47 seems to be particularly useful for obtaining leaf rust resistance in T. boeoticum and T. urartu species.

Genetic diversity of Czech apple cultivars inferred from microsatellite markers analysis

J. Patzak, F. Paprštein, A. Henychová, J. Sedlák

Hort. Sci. (Prague), 2012, 39(4):149-157 | DOI: 10.17221/218/2011-HORTSCI

Genetic diversity and genetic relationships of Czech apple cultivars were evaluated. Trees of 33 Czech apple cultivars and 97 reference foreign cultivars were analysed using the set of 10 SSR (simple sequence repeat) primer pairs. The total of 89 polymorphic alleles were amplified, while the number of alleles per locus ranged from 4 to 14. The SSR dendrogram, based on the Jaccard's similarity coefficient, divided apple cultivars into three major groups: Cox's Orange Pippin, McIntosh and Golden Delicious ancestries. The clustering highly depended on pedigree and origin of apple cultivars. Spontaneous mutated cultivars were identical with their progenitors. We proved that microsatellite markers were useful for evaluation of genetic resources, collection management and cultivar identification.

Possibilities of some methods for risk assessment of arsenic load in soils

R. Vácha, H. Macurová, J. Skála, M. Havelková, J. Čechmánková, V. Horváthová

Plant Soil Environ., 2008, 54(7):279-287 | DOI: 10.17221/419-PSE

The paper deals with the possibilities of arsenic analysis in the soil. The aim is the selection and verification of the methods for analysis of As in the soil, which seem to be suitable for risk evaluation based on the increased As soil load. Economical and analytical availability of the methods is an important criterion. A sample set of 6 heavily contaminated soils was used to compare application potential of prevalent as well as less common procedures for determination of arsenic forms in soils. The results showed that substitution of analytically more demanding As total content analysis and sequential analysis for simple extraction using one-off agents seemed to be promising in many cases. The comparison and evaluation of statistical correlation of used methods of As analysis in the soil was realised. The results suggested that using the combination of simple 1M NH4NO3 extraction and determination procedure for amorphous Fe forms in the soil (the Tamm method) was suitable for the risk assessment stemming from the As contamination without necessity of the application of more complicated sequential extraction procedures.

Healthy life style and buying behaviour in the Czech Republic

M. Foret, J. Paděra

Agric. Econ. - Czech, 2008, 54(7):307-313 | DOI: 10.17221/2707-AGRICECON

The authors analyze and interpret the data of the Czech Statistical Office about the consumption of the selected foods in the form of secondary analysis. The effort was to take into account the assessment of the trends as well as to deduce their possible impact on the health condition of an individual. From the analyses mentioned, it is obvious that in the selected statistical data of the development of food and beverages consumption in the Czech Republic the tendencies towards healthy life style have not been unambiguous or significant within the last eight years.

Quantification of nutrient content in the aboveground biomass of teak plantation in a tropical dry deciduous forest of Udaipur, India

J. I. Nirmal Kumar, R. N. Kumar, R. Kumar Bhoi, P. R. Sajish

J. For. Sci., 2009, 55(6):251-256 | DOI: 10.17221/107/2008-JFS

This study was designed to evaluate the quantification of the nutrient content of aboveground biomass of teak plantation in a tropical dry deciduous forest of Udaipur, Rajasthan, India. The nutrient contents in the total biomass of teak in the plantation were 165.47 kg/ha N, 20.96 kg/ha P, 35.06 kg/ha K, 49.29 kg/ha Ca, 31.52 kg/ha Mg, 4.27 kg/ha Na, 4.06 kg/ha S and 3.21 kg/ha Cl. In total, 42.93% of the dry matter accounted for crown biomass (leaves, branches, twigs and reproductive parts), which in turn accounts for 60.93% N, 58.63% P, 54.30% K, 51.40% Ca, 62.5% Mg, 53.62% Na, 59.85% S and 60.74% Cl of the aboveground biomass, whereas 57.07% of the dry matter account for trunk biomass (bole bark and bole wood), which in turn accounts for 39.07% N, 41.37% P, 45.70% K, 48.6% Ca, 37.5% Mg, 46.38% Na, 40.15% S and 39.26% Cl.

Selenium metabolism in goats - maternal transfer of selenium to newborn kids

L. Misurova, L. Pavlata, A. Pechova, R. Dvorak

Vet Med - Czech, 2009, 54(3):125-130 | DOI: 10.17221/3088-VETMED

The aim of our study was to compare the concentration of selenium (Se) and the activity of glutathione peroxidase (GSH-Px) in the whole blood of goats and their newborn kids. The experiment involved 25 gravid, clinically healthy goats of the white shorthaired breed. On the day of delivery, we took whole blood from the mother and her newborn kid before the kid drank the first colostrum. In mothers, the measured average concentration of Se in whole blood was 149.60 ± 45.01 μg/l, the average concentration of Se in kids was 87.91 ± 29.66 μg/l. Average activity of GSH-Px in the blood of mothers was 938.46 ± 341.09 μkat/l, and in the blood of kids 658.20 ± 339.13 μkat/l. Regression and correlation analysis produced regression line formulas and correlation coefficients that revealed a close, statistically significant relation (P < 0.01) between the concentration of Se in the blood of mothers and their kids and the activity of GSH-Px in mothers and their kids. The relation between the concentration of Se in the blood (μg/l) of mothers and kids was γ= 0.484x + 15.55; r = 0.73, the relation between the activity of GSH-Px in blood (μkat/l) of mothers and their kids was γ = 0.809x - 101.27; r = 0.80. The concentration of Se and activity of GSH-Px was lower in newborn kids than in their mothers, reaching approximately 60-70% of the mother's levels. The relation between the concentration of Se and activity of GSH-Px in the blood of goats was γ = 4.23x + 276.31; r = 0.64 (P < 0.01) and the relation between the concentration of Se and the activity of GSH-Px in the blood of kids was γ = 6.556x + 64.70; r = 0.83 (P < 0.01). It follows that a Se concentration of 100 μg/l corresponds to a GSH-Px activity of 699.51 μkat/l in the blood of mothers and 720.34 μkat/l in the blood of kids. The results show the need to provide for a sufficient Se saturation of goats with a view to preventing Se deficiency in kids and that the Se concentration in the blood of newborn kids is physiologically about 40% lower in comparison with the Se concentration in the blood of their mothers. This should be taken into account when interpreting the results and assessing the reference values of Se concentration in the blood of kids.

Masked Mycotoxins: an Emerging Issue for Food Safety

G. Galaverna, C. Dall'Asta, M. Mangia, A. Dossena, R. Marchelli

Czech J. Food Sci., 2009, 27(10):S89-S92 | DOI: 10.17221/1064-CJFS

The presence of masked or hidden forms of Fusarium mycotoxins (deoxynivalenol, DON, zearalenone, ZEN and fumonisins B1, B2 and B3) were studied in wheat and maize derived products. Significant amounts of these forms were found both in raw and in processed food commodities. Deoxynivalenol-3-glucoside was found in wheat products up to 30% of DON concentration. Bound forms of fumonisins often account for an equal or even higher amount in comparison with the free forms.

Identification of heterosomes in spermatoza of rams with 54,XX/54,XY chimerism

A. Kozubska-Sobocinska, B. Rejduch

Vet Med - Czech, 2008, 53(5):250-254 | DOI: 10.17221/1855-VETMED

The aim of the study was to identify heterosomes in the semen of three Romanov rams - carriers of leukocyte chimerism (FISH technique) and to determine the proportions between spermatozoa with X and Y chromosomes. The choice of bovine probes for hybridization with ram heterosomes was dictated by genetic conservatism of bovine and ovine heterosomes. The ratio between spermatozoa with a yellow fluorescent signal containing the X chromosome in the haploid set and spermatozoa with a red-purple signal indicating the presence of the Y chromosome, taking into account spermatozoa with no signal, was 52%:43%:5% in ram No. PL100006077676; 47%:44%:9% in ram No. PL100006078031; and 48%:46%:6% in ram No. PL100006078895. The results obtained lead us to conclude that the 54,XX/54,XY chimerism has no effect on sex ratio in offspring.

Determination of leaf rust resistance genes Lr10, Lr26 and Lr37 by molecular markers in wheat cultivars registered in the Czech RepublicShort Communication

Alena HANZALOVÁ, Taťána SUMÍKOVÁ, Pavel BARTOŠ

Czech J. Genet. Plant Breed., 2009, 45(2):79-84 | DOI: 10.17221/28/2009-CJGPB

Twenty-seven winter wheat cultivars registered in the Czech Republic were tested by molecular markers for the presence of Lr26 and Lr37, and twenty-eight cultivars for the presence of Lr10. Gene Lr37 was determined in eleven cultivars, gene Lr10 in ten cultivars and gene Lr26 in four cultivars. Eight cultivars had combinations of two Lr genes, one cultivar possessed all the three Lr genes. The results of marker analyses were compared with multipathotype analysis which confirmed the presence of Lr26 but did not enable the verification of the presence of Lr10 and Lr37. Seedling resistance was compared with resistance of the studied cultivars in the field.

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