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The prevalence of thermophilic Campylobacter spp. was evaluated in the caecum and on carcasses of pigs at slaughter and in the facilities of slaughterhouses in the period of 2001- 2003. During that timeframe, prevalence of Campylobacter spp. in both the pigs and the environment of slaughterhouses decreased. In 2001, Campylobacter spp. were detected in 34% of 316 samples; in 2002 there were 27% of positive findings out of the 624 samples; and in 2003, Campylobacter spp. were detected in 16% out of 300 samples. Campylobacter spp. were mostly found primarily in the caecum (292 isolates) and in smears collected from carcasses (21 isolates), while Campylobacter spp. were isolated only sporadically from the work surfaces of equipment in slaughterhouses. The majority of isolates were identified as C. coli. In 2001, 16 out of 109 strains of Campylobacter spp. were identified as C. jejuni; in 2002, 8 out of 167 strains were C. jejuni; and in 2003, none of 47 isolates was identified as C. jejuni.

Probiotics have been used with increasing frequency in nutrition and for prophylactic purposes during the last years. In the present study we investigated the effect of Lactobacillus fermentum AD1 - canine isolate on selected intestinal microbial groups, weight gain, organic acids, haematology, glutathione peroxidase and phagocytosis of leucocytes in 2-days-old Japanese quail (Coturnix coturnix japonica). The results demonstrated that the 4-day application of this strain significantly increased the population of lactic acid bacteria - lactobacilli and enterococci in faeces (P < 0.01 and/or P < 0.001) and caecum of quail (P < 0.001) and significantly decreased the counts of E. coli in faeces (P < 0.05). The daily weight gain was increased by 14%. Although intestinal pH of both groups of birds was similar, the concentration of lactic acid was significantly increased in the experimental group (P < 0.05). The concentration of other organic acids (acetic, acetoacetic, formic, succinic, valeric, propionic, butyric) as well as blood glutathione peroxidase was not influenced. The index of phagocytic activity of leucocytes was significantly improved (P < 0.01).

The evaluation of conditions for farming of agricultural enterprises in sub-mountain and mountain areas of South Bohemia results from the analyses of material and physical indicators and characteristics. The analyses of farmland fund and its exploitation, plant and animal structure and physical results converted into money enable to identify and assess the state and development of relations which affect the economic results of these agricultural enterprises.

The paper deals with the analysis of current situation in sales of selected bio-products in the conditions of the Czech Republic. It is focused on the structure of plant and animal products, sold quantities, product prices, conditions of sale, common distribution channels and so on. Selected organic products are divided into two groups for the purpose of this analysis - products coming from producer to consumer through distributor and products coming to consumer through processor and distributor.

The article presents the analysis of regional distribution for the approved and completed projects by 2004 under the SAPARD programme in the Slovak Republic. The distribution of funding strongly favoured powerful (innovative) business subjects which increased their competitiveness and gained the "advantage of technology advance" (which, in time may change into the rent of technology advance) associated with growth in labour productivity and high probability of investment return. The implementation of the SAPARD programme provides a strong impetus to the restructuring of businesses in agrifood industry, it accelerates and improves the selection among the businesses and economical differentiation. The bulk of the approved funding for projects under the individual measures for agriculture and processing industry within the SAPARD programme was distributed among western and southern regions of Slovakia. The implementation of the SAPARD programme, on one hand, has helped to reduce regional disparities in the vertical spatial approach by the diversion of the bulk of funding to the economically less favoured southern areas of Slovakia lagging behind in terms of infrastructure. On the other hand, though, it has increased the regional disparities in the horizontal spatial view with regard to the fact that most of the funding is spent in the developed regions of western Slovakia. Eastern Slovakia thus has become a disadvantaged and minority recipient of the financial assistance under this programme.

Fusarium head blight (FHB) is a barley disease, which occurs every year in various areas of barley cultivation all over the world and the increasing incidence has been confirmed in the Czech Republic also during the last years. We aimed to emply AFLP (Amplified fragment length polymorphism) and SSR (Single sequence repeats) markers to describe diversity among breeding lines with a sufficient level of resistance towards FHB and to find marker(s) associated with the analysed traits. The number of eight accessions including five expected resistance donors and three sensitive lines were tested in the field and laboratory. The field values and the amount of deoxynivalenol were positively correlated (r = 0.92). The laboratory test and content DON manifested also a high correlation (r = 0.73). Several DH lines developed from androgenetic barley progenies of the F1 hybrids between the susceptible line PI 383933 and resistant line PEC 210 or the susceptible line PI 383933 and resistant cultivar Chevron were found resistant towards Fusarium infection in both the field and laboratory tests. Low infestation was found at line DH 37 from combination Chevron × PI 383933 and lines DH48, DH49, DH50 and DH55 from the combination PEC 210 × PI 383933. Cluster analyses based on 68 AFLP and 18 SSR markers demonstrate a genetic relationship among parental genotypes and DH lines.Some DH lines combined a sufficient degree of resistance against FHB and extract content (basic parameters malting quality). Statistically significant differences in malt-extract values were observed between groups of the DH lines possessing and not possessing the AFLP marker CAA/AGC 341bp. The markers will be further evaluated and optionally used for MAS.

The tree species composition of forests in the Czech Republic has changed due to the human impact over the time. Nowadays, the idea of reducing the area with spruce stands and of increasing the area with broadleaved stands is widely discussed. The paper compares the target species composition with the presentone and creates four groups of areas with the largest and/or negligible differences between the target and the actual tree species composition using the Czech typology school.

A decline of macromycetes, especially of ectomycorrhizal species, has been described in the last decade in various parts of Europe. These changes are attributed to direct or indirect effects of air pollution. In Central Europe these changes correlate with visual damage of forest. Many ectomycorrhizal fungi seem to be very suitable bioindicators of the disturbance of forest ectotrophic stability. Stages of this disturbance can be linked directly to particular phases of impoverishment of ectomycorrhizal mycocoenoses. These conclusions have been obtained from long-term research on permanent plots (1,000-2,500 m²) in spruce and oak forests in the Czech Republic, but it seems that they are valid generally. For the quantification and study of mycorrhizal activity a special method has been developed and applied. The analyses of mycorrhizas from the same plots in the period 2000-2002 indicate large mycorrhizal dynamics. These data correlate with data obtained from a fruiting bodies survey. Both the percentage of ectomycorrhizal species and the ratio of active mycorrhizas are highly sensitive to outer impacts (air pollution, acidification, fertilization). Their decrease is in correlation with the strong defoliation of trees and can be used for the prediction of further development in comparable stands.

The aim of this study was to explore the use of NIR spectroscopy of laboratory milled flour to predict the milling characteristics of wheat. Quantitative traits of the milling process of wheat were predicted by analyses of NIR spectra of six sets consisting of 94 samples. Reference data were obtained by grinding the samples on the laboratory mill Chopin CD1-auto (France), spectral data were measured on spectrograph NIRSystem 6500. Commercial spectral analysis software WINISI II was used to collect spectra, develop calibration equations and evaluate calibration performance. The quality of prediction was evaluated by coefficients of correlation between the measured and the predicted values from cross and independent validation. MPLS/PLS regression and ANN methods were used. A statistically significant dependence (at the probability level of 99%) was determined for all traits studied in the case of cross-validation. Satisfactory accuracy of the prediction models by independent validation was achieved only for semolina extraction rate, models for other characteristics did not show acceptable precision.

The bacterium Pseudomonas savastanoi pv. nerii was identified as the causal agent of parenchymatous galls on leaves of potted oleander plants grown at Brno, Moravia, in 2004. The plants had originated from cuttings made from firm shoots of a supposedly asymptomatic plant grown in and introduced from the Mediterranean region. The Biolog GN microplate system was used to identify the isolated bacterial strains. Successful inoculation of Nerium oleander seedlings proved the pathogenicity of the isolates. This is the first record of P. savastanoi pv. nerii in the Czech Republic. :

The evaluation of effects of some non-genetic factors (sex, litter size, age of dams and year of study) on the growth of 85 kids of the Brown Short-haired breed was carried out in a period of two successive years. The analysis of the growth of all kids revealed that in the time interval from birth to Day 90 of age daily gain and final body weight were 181.3 g and 19.52 kg, respectively. As far as the effect of sex was concerned, the intensity of growth was significantly higher in males than in females (P ≤ 0.01) in all periods under study. From birth to Day 90, the respective daily gains of males and females were 200.6 g and 162.0 g. As far as the litter size was concerned, it was found out that daily gains of singles and triplets were higher than those of twins and that the differences between single kids and twins were significant (P ≤ 0.01) in all periods under study. Specifically, daily gains of single kids, twins and triplets in the time interval from birth to Day 90 were 201.4 g; 167.1 g and 175.6 g, respectively. The analysis of the effect of age of dams on daily gains revealed significant effects of this factor on daily gains in the time intervals of Day 30 - Day 60; Day 60 - Day 90; Day 30 - Day 90 and from birth to Day 90 (P ≤ 0.05). With the exception of the period from birth to Day 30, the highest daily gains were recorded in kids of four-years-old dams in all other periods under study. In both years of study, all kids were reared under nearly identical conditions but the effect of year of study on the growth was significant.

Haemolytic products arising in chronic hemophthalmos cause cellular infiltration, necrosis of the vitreous structure, and fibrous membrane formation. In this process, retinal pigment epithelium plays an important role for its antioxidant properties and the capability to phagocyte the decay products.

A total of 245 isolates of Actinobacillus pleuropneumoniae included in this study were isolated from lung tissues of pigs which died of porcine pleuropneumonia. The isolates were obtained from 52 swine herds in the Czech Republic over the period 2003-2004. All the serotypes were serotyped by the coagglutination test. All isolates with cross-reactivity between serotypes 9 and 11 or 1, 9, and 11 in the coagglutination test were further serotyped by the quantitative coagglutination test. The results of serotyping showed that serotype 9 was dominant (46.5%) followed by serotypes 2 (18.5%), and 11 (14.2%). Two-way cross-reaction between serotypes 9 and 11 was found for 7.5% of the isolates. The serotypes 4, 5, 7, and 12 were found infrequently (2.4%). The isolates that could not be serotyped (11%) were classified by PCR typing system based on the apx and omlA genes into the serotype groups 1, 9, 11 (7.5%); 2, 8 (2.3%), and 7, 13 (1.1%).

Sustainable development in regarded as the issue and method of solution of a number of global problems, which origined namely in relation to dynamic industrial development and the prominent growth of world population. Sustainable development is, above all, the concept closely related to human attitudes and values. The contribution presents the definition of sustainable development, its modern concept, sustainable development dimensions.

Virtual portal (VIPO) is designed to provide users with a single-point access to information, and tools to support their decision-making. VIPO illustrates the best practice presented in terms of case studies coded along three dimensions: field, domain and objectives. The second major source of knowledge is based on software packages made available to users including tutor support. The third part of the portal provides useful links to the existing databases which have the potential to facilitate decision making in agribusiness. All three pillars of the VIPO are complemented by on-line consultancy services.

During the construction of model logging costs for valuation of forest stands, by accident I found out differences between some species included in the groups of tree species. Differences within the groups of species may cause errors in logging costs of some species, for example with hornbeam, all species of linden, all species of rowans and horse chestnut. With the help of simple calculations it was proved that the differences could be very large, that they were more than forty per cent, it means they were significant. On the basis of my further research it is envisaged to increase the number of groups of trees from 13 to 16. The purpose is to give the most accurate background to make up a model of logging costs. In the second step it is expected that the model can provide the results for more or fewer groups of trees more easily if statistical methods are used. But this problem is not a part of this paper.

The paper summarises main ideas concerning the structure of natural forest ecosystems at middle altitudes and documents the changes of humus forms and their chemistry in the natural forest of Žákova hora National Nature Reserve, in typical selected parts with different tree species composition: European beech - Norway spruce. The species composition, age and spatial structure are discussed and analysis of the uppermost soil layer is done in particular parts of the natural forest regeneration cycle, of different stages respectively. We compared the amount and layer composition of surface humus and basic pedochemical characteristics of holorganic and upper mineral horizons. The results document changes in the character of humus forms as a consequence of the tree species change. On the contrary, a high portion of uncertainty as for the species, age and spatial structure follows from discussion about the structure of natural forests at middle altitudes.

ESFY phytoplasma (European stone fruit yellows phytoplasma) is nowadays one of the most important plant diseases, especially on apricots and peaches, and it belongs to the list of organisms for which quarantine is required in the Czech Republic. The aim of this study was to determine the best period for tissue extraction and the best technique for ESFY detection. It was also to investigate the possibility of isolating DNA for use in ESFY detection from the leaf-stalks of randomly chosen symptomatic and asymptomatic apricot trees. Results of the amplification of DNA extracted from leaf-stalk and phloem sampled from 2-year old woody shoots during the years 2003 and 2004 were statistically analysed and compared, and visible disease symptoms were simultaneously evaluated and compared to the results of molecular detection. DNA isolation from leaf-stalks can be considered as less significant and reliable than isolation from phloem sampled from 2-year old woody shoots.

Chrysopogon gryllus and Festuca vallesiaca are components of a number of meadow-pasture communities in Serbia. We performed the analyses of phenolics that influence quality and digestibility of grasses to a great extent. Total phenolics were measured spectrophotometrically and phenolic acids by HPLC analysis. The aboveground parts of C. gryllus contained 10.6 mg/g whereas F. vallesiaca of 21.6 mg/g total phenolics. Bound phenolics dominated over free ones in both species. The content of both free and bound p-coumaric, ferulic, p-hydroxybenzoic and syringic acid was higher in C. gryllus (6.34 mg/g) than in F. vallesiaca (3.96 mg/g). Derivatives of cinnamic acid prevailed in both species compared to the benzoic acid derivatives. Low quality of C. gryllus is connected with its high tissue phenolic acids and mediocre quality of F. vallesiaca with the high content of total phenolics that act unfavourably on digestibility of such grasses.

The present study deals with the use of high-grain diets with a low proportion of fodder for fattened cattle, and with the evaluation of their effect on the growth intensity and metabolic profile of the animals. Thirty Holstein × Czech Pied bulls were given diets containing from 86.69 to 88.54% concentrates based on crushed cereals in the period from 216^th to 327^th day of age. The growth intensity of bulls was high, with the average daily weight gain of 1.64 kg in the course of the whole experiment. When the average body weight of animals reached 343.67 kg and 450.93 kg, blood samples were taken from the vena jugularis of 10 randomly selected animals for the assessment of acid-base balance and selected biochemical parameters. Slightly decreased pH values and increased pCO₂ were detected by the assessment of acid-base balance. The calculated values of base excess and standard bicarbonate were in the reference range; however in samples of the second collection a highly significant decrease was found (P ≤ 0.01). By a biochemical analysis of blood increased levels of plasma phosphorus were detected in samples of both collections in comparison with the accepted reference range. A statistically highly significant increase (P ≤ 0.01) in plasma urea concentrations was detected in samples of the second collection. Other investigated parameters ranged within the accepted reference values. The results of the experiments show that high-grain diets produced intensive growth with high daily weight gains, without adverse effects on the health status of the investigated bulls. Although some depletion of compensatory mechanisms maintaining the acid-base balance was recorded, no serious disturbance of metabolic profile was registered in the animals.

The aim of the study was to verify under experimental conditions the hypothesis that a reliable protection of piglets against Actinobacillus pleuropneumoniae (App) infection can be obtained by colostrum-derived antibodies and/or a low-dose infection from sows naturally infected with App. Twenty-eight piglets were allocated into four groups. Piglets of groups 1A and 1B were the progeny of App-seronegative sows, piglets of groups 2A and 2B originated from App-seropositive sows. At the age of 4 weeks, piglets in groups 1A and 2A were exposed to a low infection dose with the pathogenic strain of App serotype 9 (4 × 10⁴ CFU/ml). At the age of 8 weeks, all four groups of piglets were exposed to the App serotype 9 challenge (2.5 × 10⁷ CFU/ml). We compared the differences in clinical, radiological, pathological changes and cytological findings in blood and bronchoalveolar lavage samples between groups with different levels of antigen-specific immunity after challenge. Piglets that obtained colostrum-derived antibodies and experienced a low-dose App infection were best protected against App challenge. More apparent clinical and pathological changes were observed in groups protected with either only colostrum-derived antibodies or active immunity induced by a preceding low-dose infection. However, none of the types of protection prevented developing of the disease and characteristic changes in the lungs. Cell counts changes in blood and lavage gave evidence of only bacterial infection in progress; however, between-group differences were not marked. Antibodies (IgG, IgA) present in the respiratory tract at the time of challenge played a major role in protection against App infection.

The political goal to reduce the transformation effects of political measures to the advantage of active farmers is only partially achieved with the Combi-model. The primary recipients are farmers who will give up their farms after introducing the Combi-model. These farmers have mostly farmed leased land. They will return these lands (leased before the reform) to the former leasers and sell their premium rights to other farm owners or lease these in co-operation with the former leasers for a fee to a new lease. Farmers who want to farm over a long term cannot use the stronger negotiating position against the old lessors following the introduction of the Combi-model because they must maintain a good leaser-leasee relationship. But they do profit to a certain extent, at least at first, because the negotiating results of the farms closing with the old leasers will become public. In contrast, the negotiating position of farmers who first lease their land areas following the introduction of the Combi-model is hardly strengthened, particularly in the current lease market in Germany.

Thymbra spicata var. spicata, which grows wildly in the flora of Turkey, is known as thyme. A field trial was conducted to determine suitable harvesting time and cutting height for Thymbra, therefore plants in the trial were harvested at two cutting heights in various stages of development in the course of the growing season (pre-flowering, full-flowering and post-flowering). It was determined that the time of harvesting had a significant effect on herbage yields, oil percentage and oil yield. The highest drug leaf yield (3.107 t/ha) was obtained in the full-flowering stage with 10 cm cutting length, essential oil percentage ranged from 1.58 to 2.33%, the highest essential oil yield (70.7 l/ha) was obtained at the full-flowering stage with 10 cm cutting length. As a result of this study, harvesting in the full-flowering stage with 10 cm cutting height was the best treatment in respect of the yield of dry matter and oil for thyme (Thymbra spicata) cultivation.

The paper is focused on the analysis of the wine demand and chosen factors which influence the wine demand in the Czech Republic, in Slovakia, in Austria, and in Germany. In the Czech Republic, the wine consumption per inhabitant went up slightly in 1994-2003, the wine demand has a rising trend. The wine demand has the rising trend in Germany as well. In Slovakia, the year wine consumption per inhabitant decreased by 14.9% from 1994 to 2003; in Austria by 7.7%. There is a downward trend of development in these countries. The influence of the analyzed factors on the development of the wine demand is different in the particular countries. The dependence is possible to follow at the total level of incomes in comparison with the consumer's price of wine.

The skin colour of 21 varieties was assessed on a molecular level. Four varieties had yellow skin colour with the genotype constitution a¹a¹, a¹a² or a²a². Seventeen varieties had dominant red colour. Homozygous A¹A¹ constitution was present in 7 varieties, and heterozygous constitution A¹a¹ or A¹a² was found in the rest of the assessed varieties. The S-alleles controlled the incompatibility system of the pistil. Their detection is possible by means of the PCR method on the basis of allele specific primers. Six S-alleles (S1, S2, S3, S5, S7 and S9) were studied in the same collection of 21 diploid Czech varieties. This paper brings new findings on S-allele characterization, because the Czech varieties have not yet been studied on a molecular level. Both types of S-allele were found in 12 varieties. Only one type of S-allele was described in 9 varieties. Simultaneously, the presence of the Vf gene was screened in the collection of 21 Czech apple varieties. Ten varieties with a field resistance against the scab had a heterozygous constitution of the Vf gene. All 11 susceptible varieties were recessive homozygous vfvf.

Cultivars, homozygous sources and lines of pea (Pisum sativum L.) resistant to Erysiphe pisi had recently been attacked by another powdery mildew species, Erysiphe baeumleri, in the field and in glasshouse conditions. Inoculation with E. baeumleri was carried out in the glasshouse to evaluate the level of resistance of 16 pea genotypes. Susceptible pea lines produced abundant conidia and cleistocarps on petioles and leaves. Only the genotype Tudor (Cebeco 4119) was found to be completely resistant to E. baeumleri. Nineteen pea genotypes (with gene er-1) were tested to natural infection by E. baeumleri in field screening trials. Only few of them demonstrated a high level of resistance (Fallon, AC Melfort and Joel). Consort R, SGL 2024, SGL 1977 and Franklin were very susceptible to E. baeumleri. Cleistocarps with 1-4× dichotomously branching apices of appendages were formed only on susceptible and very susceptible plants of genotypes SGL 444/2185, Consort R, SGL 2024, SGL 1977, LU 390-R2, Lifter, Highlight, Cebeco 1171 and Carneval R in the field and glasshouse. Susceptible control genotypes without gene er-1 (Komet, Adept and Gotik) were attacked in the trials by E. pisi only.

Forty-seven Kivircik ram lambs, aged 2 months, raised and managed under an intensive system were used to measure the development of testis diameter and length, and scrotum circumference, length and volume, and their association with the development of body growth. The effects of age and body weight of lambs, type of birth and dam age on the development of the five testicular parameters from weaning at 2-6 months of age were investigated. In ram lambs, the respective least-squares means of testis measurements: testis diameter, testis length, scrotum circumference, scrotum length and scrotum volume were 2.55 ± 0.099 cm, 5.83 ± 0.165 cm, 15.23 ± 0.650 cm, 7.24 ± 0.287 cm and 87.57 ± 5.921 cm³ at 2 months; and 2.99 ± 0.099 cm, 6.59 ± 0.164 cm, 14.24 ± 0.657 cm, 10.60 ± 0.290 cm and 157.49 ± 5.987 cm³ at 6 months. The effect of birth type on testis diameter and the effect of dam age on testis length were significant (P < 0.05 and P < 0.01), besides the effects of lamb age and live weight of lambs on the whole testis measurements were significant (P < 0.01).

During the period of 2001 and 2003 a total of 238 isolates of Actinobacillus pleuropneumoniae obtained from 26 pig herds in the Czech Republic (2001 - 73 isolates, 2002 - 110 isolates, 2003 - 55 isolates) were examined for antibiotic resistance by disc diffusion method. Resistance to erythromycin (95.5% to 98.6%), streptomycin (76.4% to 82.7%) and tiamulin (72.7%) was detected most frequently over the whole monitored period. High increase in percentage of resistant isolates to tetracycline (2001 - 11.0%, 2002 - 36.4%, 2003 - 81.8%), doxycycline (2001 - 9.6%, 2002 - 34.6%, 2003 - 61.8%), nalidixic acid (2001 - 2.7%, 2002 - 14.5%, 2003 - 45.5%), and norfloxacin (2001 - 0%, 2002 - 7.3%, 2003 - 34.6%) was found, while differences in resistance to cephalotine (2001 - 1.4%, 2002 - 7.3%, 2003 - 9.1%), cotrimoxazol (2001 - 4.1%, 2002 - 10.0%, 2003 - 7.3%), amoxicillin (2001 - 12.3%, 2002 - 12.7%, 2003 - 10.9%) and amoxicillin-clavulanic acid (2001 - 9.6%, 2002 - 9.1%, 2003 - 10.9%) were small. Prevalence of isolates resistant to sulfasomidine was detected decreasing (2001 - 43.8%, 2002 - 47.3% and 2003 - 29.1%).

Nine Bornean river turtles (Orlitia borneensis, Gray, 1873) suffering from lethargy, ulcerations and caseous necrosis of the plastron were evaluated for haematology and plasma chemistry. Intra-erythrocytic haemogregarine parasites were associated with anaemia, low haemoglobin, basophilia, eosinophilia, heterophilia and azurophilia. After eight months of treatment consisting of antibiotics, debridement and scrubbing of lesions with enilconazole or povidone iodine, rehydration, deworming and tube feeding, lymphocytes, basophils, eosinophils, heterophils and azurophils returned to the normal ranges. Haematocrit, RBC and haemoglobin concentration were under the normal ranges in 24 months. A progressive decrease in haemogregarine parasitaemia was also seen, however, haematologic changes could not be definitely attributed to these parasites.