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Results 1021 to 1050 of 2032:

Short-term effects of tillage and leaf mulch on soil properties and sunflower yield under semi-arid conditionsOriginal Paper

Koena Rivonia Selolo, Jestinos Mzezewa, Jude Julius Odhiambo

Plant Soil Environ., 2023, 69(2):55-61 | DOI: 10.17221/160/2022-PSE

A study was conducted at the University of Venda Experimental Farm, Limpopo province, South Africa to determine the effects of tillage and mulching on selected soil properties, and yield of sunflower (Helianthus annuus L.). The experiment was laid out in a split plot design with three replications during the 2018/19 and 2019/20 cropping seasons. Treatments consisted of conventional tillage (CT) and minimum tillage (MT) and three levels of avocado leaf mulch (0, 6 and 12 t/ha). Bulk density (BD), aggregate stability (AS), infiltration rate (IR), soil water content (SWC) and grain yield were determined. Tillage had no significant effect on BD in either season but influenced SWC and sunflower grain yield. CT recorded a significantly higher AS than MT during 2018/19 cropping season. Tillage × mulch interaction was significant during 2018/19 season with CT at 12 t/ha mulch recording higher AS than the MT. IR was significantly influenced by tillage × mulch interaction in both seasons with MT recording higher IR than the CT during 2018/19. Avocado mulch had no significant effect on sunflower grain yield in either season but influenced SWC in 2019/20 season. It was concluded that avocado mulch could be a relevant component of conservation agriculture but long-term studies are needed to validate the benefits observed in this study.

Citalopram in vitro metabolism in a beagle dog: A role for CYP2D15 in the production of toxic didesmethylcitalopram?Original Paper

B Rochat, E Paus, C Maitre, P Baumann

Vet Med - Czech, 2023, 68(4):135-144 | DOI: 10.17221/65/2022-VETMED

After administration of the serotonergic antidepressant citalopram (CIT) to beagle dogs, they may experience severe convulsive attacks in relation with considerably higher plasma concentrations of the metabolite didesmethyl-CIT (DDCIT), when compared to those in humans medicated with CIT. This pilot study aimed at determining the role of cytochrome P-450 (CYP450) isozymes in the in vitro metabolism of CIT to desmethyl-CIT (DCIT), and of DCIT to DDCIT in the liver microsomes of a single beagle dog. The incubations with racemic CIT or DCIT reveal a high affinity enzyme with Km between 0.3 µM and 1.4 µM for S- and R-DCIT and S- and R-DDCIT productions, respectively. In comparison to human enzymes, the intrinsic clearance values of this high affinity enzyme are between 15 µL/(min x mg of protein) and 52 µL/(min x mg of protein), i.e. very high. In vitro experiments with inhibitors suggest that CYP2D15, which shows analogy with human CYP2D6, is by far the main CYP450 isozyme involved in the production of DCIT and DDCIT whereas CYP3A12 and CYP2C21/41 showed a weak implication. These observations partly explain why in humans, plasma concentrations of the toxic DDCIT are considerably lower than those observed in dogs, after administration of CIT.

The varying promotion effects of fulvic acid with different molecular weights on the enhancement of grain yield and quality of winter wheatOriginal Paper

Yuanyuan Liang, Zeping Wang, Qiuzhe Shi, Fang Li, Zunkang Zhao, Yanlai Han, Yi Wang

Plant Soil Environ., 2023, 69(4):141-151 | DOI: 10.17221/391/2022-PSE

This study aims to verify the application effect of Fluvic acid (FA) with different molecular weights (MW) on the growth and quality of winter wheat. FA extracted from lignite was divided into 3 MWs (W1≤3000 D, 3000 D<W2≤10000 D, and W3>10000 D) by dialysis, and its structure was analyzed. Three application rates were set for each MW FA in the pot experiment, which were 10, 25 and 50 mg/kg in soil, respectively, and water was the control. There were significant interactive effects of MW and application rates of FAs. Compared with the control, all the FA treatments significantly increased grain yields, nitrogen uptake efficiencies, grain iron concentration, and soil available nitrogen concentration. Heatmap analysis revealed that the W1C2 (10 mg/kg W1) treatment had the most significant impacts for all analyzed indexes, whereas W3C3 (50 mg/kg W3) showed the weakest impacts. The results showed that at a low application rate (10 mg/kg in soil), the promotion effects of the three MW FAs were similar. W1 showed the most significant promotion effects, which was attributed to the combined effects of its lower MW and functional group characteristics.

The effect of irrigation treatments at different development stages on the bioactive components of sunflower cakeOriginal Paper

Erhan Göçmen, Sıla Barut Gök, Yasemin Erdoğdu

Plant Soil Environ., 2023, 69(4):179-187 | DOI: 10.17221/440/2022-PSE

The aim of the study was to determine the effect of water deficiency at different development stages on the bioactive content and phenolic compounds in sunflower (Helianthus annuus L.) cake, the residue left after oil is extracted from sunflower seeds. A sunflower genotype was randomly planted in a complete block design with eight different combinations of irrigation (T1–T8) by increasing the available soil moisture measured at different plant growth stages (vegetative, flowering and grain formation). Results indicated that the total phenolics of extracts varied between 1.03–2.03 times more than under drought stress (T8). The antioxidant capacity response of seed cake was 14–39% lower than under drought stress. Irrigation treatment, except in the grain formation stage, was found to enhance the biosynthesis of phenolic compounds such as vanillic and caffeic acids. Irrigation only in the grain formation stage induced the accumulation of phenolic compounds such as coumaric acid and rutin hydrate. The present study established that residues resulting from oil extraction could be converted to a polyphenol-enrichment agent for food systems by manipulating the irrigation treatments.

Effects of tea planting age on soil microbial biomass C : N : P stoichiometry and microbial quotientOriginal Paper

Guanhua Zhang, Wenjun Yang, Jiajun Hu, Jigen Liu, Wenfeng Ding, Jinquan Huang

Plant Soil Environ., 2023, 69(5):221-229 | DOI: 10.17221/164/2023-PSE

This study aimed to determine the effect of tea planting age on stoichiometric ratios of microbial biomass carbon (MBC), nitrogen (MBN), and phosphorus (MBP) and soil microbial quotient (SMQ, expressed as qMBC, qMBN, and qMBP, respectively). A chronological sequence of tea plantations (3, 8, 17, 25, and 34 years) was selected in a small watershed in the Three Gorges Reservoir Area, and a slope farmland was selected as control. The results showed that with the increase of tea plantation age, soil and microbial biomass C, N, P contents, soil C : N and C : P elevated significantly, while soil N : P overall declined; the MBC : P and MBN : P increased first and then decreased, but MBC : N varied insignificantly. The tea plantation age affected SMQ notably. qMBC first decreased and then increased following the tea planting age, while qMBN and qMBP went up in a fluctuating pattern. In this study, qMBC positively correlated with soil N : P and microbial biomass C : N : P, but negatively correlated with soil C : N and C : P; on the contrary, qMBN and qMBP negatively correlated with soil N : P and microbial biomass C : N : P, but positively correlated with soil C : N and C : P. Generally, the variations of soil microbial biomass and SMQ could reflect the soil quality of tea plantations.


Comparative study on the geographical indication protection between China and the European Union – From the perspective of the China-EU Geographical Indications AgreementOriginal Paper

Weiwen Qian, Yinguo Dong

Agric. Econ. - Czech, 2023, 69(5):185-201 | DOI: 10.17221/98/2023-AGRICECON

Geographical indication (GI) represents the specific good quality and reputation of the regional characteristics of agricultural products, which is a positive approach for stabilising the export of agricultural products, realising high quality and high prices for agricultural products and rural revitalisation. Based on the China-EU Geographical Indications Agreement, this paper discusses the differences between China and the European Union (EU) in terms of GI protection from three aspects: the institutional framework, the operational system, and the operational status of GIs. The purpose of the study is to identify the differences between China and the EU in the protection of GIs, China's shortcomings, and gaps, and to propose policy recommendations for China to protect GI products better, exploit the economic impact of GIs and enhance the competitiveness of China's exports.

A preliminary study on the root-knot nematode resistance of a cherry plum cultivar Mirabolano 29COriginal Paper

Jia Liu, Jin Zhu, Hongwen Li, Deyong Luo, Jialian Xie, Huajia Li, Shuo Liu, Yuping Zhang, Lijuan Chen, Xiaodou Xie, Dong Wang, Ke Li, Meiying Yao, Guowei Zhang

Czech J. Genet. Plant Breed., 2023, 59(3):133-140 | DOI: 10.17221/111/2022-CJGPB

Grafting is the most important means of breeding peach, plum, apricot, and other fruit trees, and the selection of the rootstock is crucial to the quality of the grafting and the yield of the products. The traditional commonly used peach rootstock is susceptible to root-knot nematode infections, resulting in a decreased yield, while a variety of cherry plum rootstocks, Mirabolano 29C, is resistant to root-knot nematode. In this study, root-knot nematode infection experiments on seedlings of traditional peach rootstocks and Mirabolano 29C confirmed that Mirabolano 29C was indeed more resistant to root-knot nematodes. At the same time, we compared the roots of the root-knot nematode uninfected and infected Mirabolano 29C by transcriptome sequencing and found 3 176 differentially expressed genes. A further functional enrichment analysis of these genes found that the secondary metabolites, phenylpropane and flavonoids, may be responsible for the high resistance of Mirabolano 29C to root-knot nematodes. These results can provide a reference value for the disease resistance breeding of rootstocks.

Immunohistochemical analysis of extracellular signal-regulated kinase expression in mature and immature bulls’ testes and epididymidesOriginal Paper

S Jang, C Yun, B Kim, S Kang, J Lee, S Jeong, Y Cho, K Sung-Ho, L Chang-Min, C Moon, K Joong-Sun

Vet Med - Czech, 2023, 68(6):231-237 | DOI: 10.17221/34/2023-VETMED

Extracellular signal-regulated kinase (ERK) has been implicated in mammalian testicular and epididymal development. This study aimed to investigate ERK expression in the immature and mature testes and epididymides of bulls. We evaluated ERK expression using immunoblot analysis and immunohistochemistry. Immunoblot analysis revealed that immature bull testes and epididymides had higher phosphorylated ERK (pERK) expression than mature bull testes and epididymides. pERK immunoreactivity was higher in immature epididymides than in immature testes. pERK was localised mostly in spermatogonia, undifferentiated sustentacular (Sertoli) cells, and interstitial (Leydig) cells in immature testes, as well as in some spermatocytes and spermatids in mature testes. In immature epididymides, the body and tail had higher pERK expression than the head, whereas pERK was broadly distributed throughout the stereocilia, basal cells, and connective tissues. pERK distribution in the head of mature epididymides was similar to that in immature epididymides, whereas few connective tissue cells were expressed in the body and tail of mature epididymides. Collectively, these results suggest that ERK is expressed in the testis and epididymis of immature and mature bulls with varying intensities, and the role of ERK in male reproductive organs may include the specific function of its development.

Improving the stability of soil aggregates using soil additives and revegetation by grasslandOriginal Paper

Markéta Mayerová, Tomá¹ ©imon, Martin Stehlík, Mikulá¹ Madaras

Plant Soil Environ., 2023, 69(6):282-290 | DOI: 10.17221/123/2023-PSE


Soil aggregate stability (SAS) is an important factor for soil quality and fertility. There are limited possibilities to influence this soil property, but one investigated method is the application of additives which have the potential to improve SAS. We established a four-year field experiment on a clay-loam Luvisol with poor soil structure to monitor SAS following the application of additives and grassland revegetation. Treatments included: (1) the untreated control; (2) compost; (3) biochar; (4) liming; (5) cattle manure; (6) woodchips; (7) woodchips + fungi inoculation; (8) pellets; (9) pellets + fungi; (10) hydrogel and (11) the change of arable land to grassland. The lowest 23.39% average SAS value was recorded for the untreated control, and then 23.92% for lime treatment, and the highest 27.69% average value was for hydrogel treatment, followed by woodchips with 27.22% and woodchips + fungi with 27.02%. A significant SAS increase of more than 200% was evident on the grassland two years after the trial’s establishment, and this was also associated with other improved physical and chemical soil properties. Finally, while most of our applied soil additives were relatively ineffective in agricultural practice, grassland revegetation is highly recommended for its rapid increase in soil aggregate stability.

Influence of 11 years of crop residue management on rice productivity under varied nitrogen levels in the rice-wheat cropping systemOriginal Paper

Rajeev Kumar Gupta, Paramjit Kaur Sraw, Jasjeet Singh Kang, Jagroop Kaur, Anu Kalia, Vivek Sharma, Surjeet Singh Manhas, Nadhir Al-Ansari, Abed Alataway, Ahmed Z. Dewidar, Mohamed A. Mattar

Plant Soil Environ., 2023, 69(7):333-343 | DOI: 10.17221/68/2023-PSE

The present study compares the six crop residue management techniques in main plots (since 2008) and three split nitrogen (N) levels, i.e., 75, 100 and 125 kg N/ha in subplots for rice crops for two years, i.e., 2019 and 2020, in sandy loam soil under field conditions. This experiment evaluated the long-term effect on rice productivity, soil organic carbon content and nutrient requirement in rice-wheat cropping system. The results revealed that different crop residue management practices and N levels significantly influenced rice growth, yield and yield attributes and improved nutrient uptake by grain and straw. Maximum grain yields of 20.8% and 17.8% higher over the conventional (no straw) treatment during 2019 and 2020, respectively, were recorded where the rice and wheat residue was retained or incorporated. The rice grain yield without residue responded significantly up to 125 kg N/ha. Whereas, with rice and wheat residue, rice grain yield did not respond to the application of N beyond 75 kg N/ha during both years.

Predispositions and challenges of agriculture from areas particularly facing natural or other specific constraints in Poland in the context of providing environmental public goods under EU policyOriginal Paper

Marek Zieliñski, Jan Jadczyszyn, Jolanta Sobierajewska

Agric. Econ. - Czech, 2023, 69(8):309-320 | DOI: 10.17221/184/2023-AGRICECON


The study’s main objective is to determine the predisposition and challenges of agriculture to provide environmental public goods to society in areas particularly facing natural or other specific constraints (ANCs) established within their current delimitation in Poland. Its organisational features, economic situation, and the degree of interest in measures specifically serving the protection of the natural environment under the Common Agriculture Policy (CAP) EU were indicated. Statistically significant factors determined farmers’ willingness to participate in these measures. It turned out that farms from ANCs communes with particularly difficult conditions significantly contribute to the total production potential of agriculture in Poland. However, compared to farms with better natural conditions, they are characterised by a lower income per 1 ha of utilised agriculture area (UAA) and a higher share of subsidies, including those for participation in environmental measures, which were implemented to a greater extent. Based on the logistic regression model, it was found that in ANCs communes with particularly difficult conditions, the most important factor of farmers’ greater willingness to implement environmental measures was their higher level of education. On the other hand, this inclination was negatively affected by the higher income adjusted for operating subsidies per 1 ha of UAA.

High pressure processing for the production of vegetable baby puree with enhanced nutritional, microbial, and sensory qualitiesOriginal Paper

Farah Javed, Hafiz Muhammad Shahbaz, Waqas Ahmed, Habib ur Rehman

Czech J. Food Sci., 2023, 41(4):263-270 | DOI: 10.17221/2/2023-CJFS


High-pressure processing (HPP) was used as a post-processing treatment for vegetable puree. Microbiological, physicochemical, nutritional, and sensory analyses of puree were investigated at room temperature. HPP (600 MPa, 5 min) was compared with thermal treatment (117 °C, 30 min) and fresh samples. Treatments did not change pH or total soluble solids. For both methods treated samples exhibited a lower microbial count (< 1.0 log CFU·g–1) over storage, compared with fresh puree. During storage, other parameters, including total phenolic contents and antioxidants also demonstrated similar or better performance than controls (P < 0.05). Overall, HPP-treated puree received a higher sensory evaluation score. Thus, HPP can be used as an alternative processing technology to improve nutritional quality and microbial safety.

Changes in the behaviour of Czech Fleckvieh dairy cows during the day under optimal ambient temperature conditions in the barnOriginal Paper

Milan Veèeøa, Stanislav Navrátil, Tomá¹ Kopec, Daniel Falta, Gustav Chládek, Francois Stefanus Lategan

Czech J. Anim. Sci., 2023, 68(9):365-371 | DOI: 10.17221/23/2023-CJAS

In the experimental section of the barn (which made up 25% of the barn), 103 cubicles in free stall barn were selected in three rows, housing 98 dairy cows of Czech Fleckvieh cattle. Monitoring was done for one calendar year, once a week. From this period, 16 days with temperatures from 0.5–15.0 °C were selected for quantification. Observations were continuously recorded at 10:00 am (one hour after the coming of the last cow from the milking parlour), at hourly intervals until 7:00 pm. Ten observations were carried out every day of the monitoring period. In total, 15 680 individual observations were recorded. The microclimatic parameters (temperature, relative humidity) were monitored within the barns continuously at 15-minute intervals using three data logger sensors. Changes in the proportion of lying to standing dairy cows were observed during the day. A steady trend of a reduced ratio of lying dairy cows during the day (from 70% to 40%) in favour of standing dairy cows was observed. The optimal value of the cow comfort index – CCI (85%) was not reached in any monitored part of the day. The number of dairy cows lying on their left sides increased from 50.4% to 56%, especially after feeding (at 4:00 pm). Differences were found in the proportion of lying and standing cows (< 0.05) at an optimal microclimate during the day. A significant preference for the left side when lying down was also found.

Effects of drought stress at different stages on soluble sugar content of soybeansOriginal Paper

Shoukun Dong, Xinyu Zhou, Zhipeng Qu, Xiyue Wang

Plant Soil Environ., 2023, 69(11):500-511 | DOI: 10.17221/385/2023-PSE

Drought is an important agricultural problem worldwide, which seriously affects the growth and yield of crops. To explore the effects of different degrees of drought on the soluble sugar content of soybeans, this study measured the soluble sugar content of two soybean cultivars at three growth stages under four levels of drought stress. The results showed that drought stress significantly affected the soluble sugar content, and there were differences among different growth stages and cultivars. At the seedling and flowering stages, the sucrose content of both Heinong44 and Heinong65 showed an unimodal trend and reached the maximum value at moderate drought. The increase rate was the highest in the leaves at the flowering stage, which increased by 36.18% and 25.79% compared with CK, respectively. The fructose and glucose contents were the highest during severe drought, and the fructose content increased the most in the leaves at the seedling stage, which increased by 18.05% and 17.67% compared with CK, respectively. The glucose content increased the most in the petioles at the flowering stage, reaching 40.66% and 35.24%. At the pod-filling stage, the three sugar contents of both Heinong44 and Heinong65 were the lowest at severe drought, and the sucrose and fructose contents decreased the most in the petioles, which decreased by 21.66% and 23.94%, 12.58% and 13.49% compared with CK, respectively. The glucose content decreased the most in the stems, which decreased by 11.72% and 9.66%. In addition, at each growth stage and drought treatment, the ratio of the soluble sugar content of Heinong44 was higher than that of Heinong65.

Changes in soil properties due to land reclamation and climate change in South Moravian floodplain forestOriginal Paper

Lubo¹ Sedlák, Soham Basu, Lubica Pospí¹ilová, Alois Prax, Jiøí Kulhavý, Jakub Prudil, Hana Hornová, Tomá¹ Vichta

Soil & Water Res., 2023, 18(4):227-235 | DOI: 10.17221/34/2023-SWR

Land use changes together with riverbed regulations to avoid the annual floods affect the ecosystem of floodplain forests. Later subsequent revitalization measures, transboundary controlled river management, wetland restoration, and integrated planning were realized to reduce the negative effect of groundwater dropping and other environmental problems. This study aimed to follow the dynamic of groundwater level, soil properties and forest vitality as affected by climate change. The continuous dataset (2019–2022) of soil physical and hydrophysical parameters and tree radial growth parameters were obtained. Groundwater level was evaluated by z-score and the means, and standard deviation values were considered. The monthly assessment of soil and climatic conditions showed that the uneven distribution of rainfall and the increase in temperatures have significantly affected the soil hydrological regime and forest growth. Continual monitoring is necessary to prepare projection models, which can help better understand both the soil and tree growth parameters in the changing environment.

The importance of agriculture and rural areas for the future in the European Union: An exploration of public opinionOriginal Paper

Mercedes Rodríguez, José Antonio Camacho

Agric. Econ. - Czech, 2023, 69(10):394-403 | DOI: 10.17221/115/2023-AGRICECON


The last years have seen a momentous transformation in Common Agricultural Policy (CAP). The focus of interventions has shifted from production toward rural development but recognising the role of farmers. Although the attitudes of farmers to CAP have long been previously studied, little attention has been focused on the views of citizens. Using data from the ‘Special Eurobarometer 520: Europeans, Agriculture and the CAP’, we sought to provide a clearer understanding of citizens’ opinions about the future of agriculture and rural areas in the European Union. In particular, we assessed rural-urban differences and examined disparities across countries, paying attention to the importance of rural development in the planned expenditure under CAP 2023–2027. The results showed that the perceived importance is higher for respondents living in rural areas and that there are disparities across countries. From a policy perspective, targeted efforts should be made in each country to address the specific challenges each country faces.

Operating performance of manual, semi-automatic, and automatic tractor guidance systems for precision farmingOriginal Paper

Paola D'Antonio, Andi Mehmeti, Francesco Toscano, Costanza Fiorentino

Res. Agr. Eng., 2023, 69(4):179-188 | DOI: 10.17221/5/2023-RAE

Precision agriculture is increasingly relying on tractor auto-steer systems to boost productivity and optimize crop inputs. Identifying field variations and performance, on the other hand, is necessary for giving site-specific recommendations. This study reports the field operating performance indicators of manual (MG), semi-automatic (SG), and automatic (AG) tractor guidance for weed control in wheat production in Southern Italy. Performance indicators include effective worked area, overall working time, effective field capacity, field efficiency, fuel consumption, and product usage. The SG tractor guidance working times were similar to the MG, but with significant savings in the herbicide spray solution and work quality. In terms of all parameters examined, the AG outperformed the SG and MG. The AG was 54% faster than the MG, resulting in an increased area worked and effective field capacity of 5 and 46%, respectively. The total time (effective time plus non-productive time) was reduced by 28%, while overlapped areas by 88.9%. Herbicide and fuel input was reduced by 30 and 11.5%, respectively. A streamlined environmental analysis indicated that AG could reduce the energy and carbon intensity of the one-time weed control process by 25 and 27% for each hectare. Our results confirm that auto guidance provides numerous benefits (e.g., machining uniformity, increased work quality, reduced resource use, and reduced environmental burdens), supporting the larger goal of agricultural production sustainability.

Yield of sweet corn and sunflower as affected by different cultivation methods and fertilisation schemesShort Communication

Attila Vad, András Szabó, Oqba Basal, Szilvia Veres

Plant Soil Environ., 2023, 69(10):480-485 | DOI: 10.17221/292/2023-PSE

If appropriately applied, tillage can positively affect the crop’s yield by enhancing the soil’s physical properties. Fertilisation also has beneficial effects on yield if applied at efficient rates by increasing available-to-uptake nutrients and improving soil’s chemical properties. A field experiment was carried out in Debrecen, Hungary, to evaluate the individual and the interaction effects of these 2 factors on sunflower and sweet corn. The cultivation methods applied were ploughing (C1), direct drilling (C2) and deep loosening (C3). In addition, 3 fertilisation rates were applied to each cultivation system: F1 (control, no fertilisation); F2 (100 : 50 : 70) kg NPK/ha and F3 (150 : 100 : 120) kg NPK/ha in a randomised complete block design (RCBD) with 4 replicates. Our results showed that fertilisation did not affect the yield of sweet corn measurably. However, C1 resulted in the highest yield, whereas C2 and C3 resulted in relatively similar yields. In sunflowers, the yields of both C1 and C2 were higher than that of C3. No significant differences were recorded between C1 and C2. The yields of both F2 and F3 were higher than the yield of F1. The differences between F2 and F3 were insignificant. It could be concluded that the cultivation method is a determining factor in the yield of sweet corn. The effects of both fertilisation and cultivation treatments were more detectable in sunflowers. The differences between the two fertilisation rates (F2 and F3) were insignificant in both species, indicating that the extra fertilisation levels might be unnecessary.

Anti-coccidial effects of dietary chamomile against experimentally induced coccidiosis in broiler chickenOriginal Paper

Sleman Said Mohammed Beski

Czech J. Anim. Sci., 2023, 68(1):30-43 | DOI: 10.17221/160/2022-CJAS


A 4 × 2 factorial experiment was conducted to investigate the effect of dietary supplementation levels (0, 5 and 10 g/kg) of chamomile flower powder and a level (60 mg/kg) of salinomycin on performance and gut health of broiler chickens under coccidiosis challenge. A total of 320-day-old Ross 308 broilers were assigned to eight treatments of four replicates with 10 birds. Oral inoculation of the challenged groups with Eimeria tenella occurred on day 8. On day 10, disease challenge and additive level significantly (P = 0.003) interacted, decreasing the feed intake. Feed intake significantly (P ≤ 0.001) decreased in birds supplemented with 10 g/kg of chamomile. At 35 days, birds fed chamomile and anticoccidial significantly (P = 0.001) increased body weight (BW) and weight gain (WG). Disease challenge adversely influenced (P = 0.001) BW and WG. Additive level and disease challenge significantly (= 0.001) interacted on feed conversion ratio (FCR). Anticoccidial and chamomile improved (P = 0.001) FCR of birds regardless of the rearing conditions. Significantly (P = 0.001) better FCR was observed in the unchallenged birds. The interaction of experimental factors was significant (P ≤ 0.04) on the relative weight of bursa. Disease challenge significantly (P = 0.005) increased the relative weight of liver whereas that of bursa decreased. Cholesterol (P = 0.002), albumin/globulin (P = 0.009), aspartate aminotransferase (AST) (P = 0.002) and alanine transaminase (ALT) (P = 0.001) significantly increased in challenged birds. The interaction of experimental factors was significant (P = 0.002) on villus length, crypt depth and villus height/crypt depth ratio (P = 0.001). Longer (P = 0.001) villi were found in anticoccidial and chamomile supplemented birds. Coccidiosis adversely (P = 0.001) influenced the jejunum morphology. Crypt depth decreased and villus height/crypt depth increased in chamomile offered birds (P = 0.001) regardless of the challenge conditions. Bursal morphology was significantly influenced by experimental factors. Dietary supplementation of chamomile had positive effects on broiler performance, immunity and intestinal morphology during exposure to the E. tenella parasite. Chamomile could be used as a potential natural anticoccidial in broiler nutrition.

Development and evaluation of a low-cost evaporative cooling system for agricultural product storageShort Communication

Natesan Kapilan, Vijay Kumar Patil

Res. Agr. Eng., 2023, 69(1):48-53 | DOI: 10.17221/41/2021-RAE

Agricultural products are highly perishable and, hence, we need to preserve these products after harvest. India is an agricultural country and, thus, the post-harvest storage of perishable agricultural products is important to reduce the gap between the demand and supply. Cold storage technologies have been developed and are used in India; however, these technologies are not popular in rural and remote areas due to the higher initial cost and the electrical energy requirement. Therefore, a number of low-cost technologies have been developed and, among these technologies, the evaporative cooling technology is gaining in popularity due to its simple design and lower initial cost. In this work, we have developed and tested a solar photovoltaic (PV) powered evaporative cooling system and used coconut coir as the cooling medium and compared the results with celdex pad. From this work, we observed that this system is an economical and efficient in reducing the temperature and increasing the relative humidity for the storage of agricultural products.

Challenging the problematic detection of clostridial isolates causing late-blowing defect with MALDI–TOF MSOriginal Paper

Pelin Ertürkmen, Zübeyde Öner

Czech J. Food Sci., 2023, 41(1):36-43 | DOI: 10.17221/199/2022-CJFS

The present study aimed to evaluate the Clostridium spp. counts in corn silage, raw milk and Kaºar cheese and to identify the clostridial isolates causing a late-blowing defect (LBD) potential using matrix-assisted laser desorption-ionization-time of flight mass spectrometry (MALDI-TOF MS). Lactate-fermenting clostridial spores (LFCS) were determined by the most probable number method (MPN) in 14 samples of corn silage, 12 samples of raw milk and 20 samples of Kaºar cheese. 181 isolates were obtained from MPN gas-positive tubes. Gram staining, catalase and oxidase activity, anaerobic development tests and Scanning Electron Microscopy (SEM) imaging showed that 95 isolates were typical clostridial bacteria. Sixty-six isolates could maintain viability during the passage and stock stages. A confirmatory identification technique was then performed using MALDI-TOF MS. The results revealed that (49 out of 66 isolates) of bacteria were correctly identified as 38 (77.55%) Clostridium sporogenes, 6 (12.24%) Clostridium butyricum, 3 (6.12%) Clostridium beijerinckii, 1 (2.04%) Clostridium bifermentans and 1 (2.04%) Clostridium sartagoforme. This study determined that clostridial isolates that cause LBD can be identified successfully and quickly by MALDI-TOF MS, a novel method for detecting anaerobic bacteria.

Development and application of a low-priced duplex quantitative PCR assay based on SYBR Green I for the simultaneous detection of porcine deltacoronavirus and porcine sapelovirusOriginal Paper

SJ Lu, MY Ma, XG Yan, FJ Zhao, WY Hu, QW Ding, HJ Ren, YQ Xiang, LL Zheng

Vet Med - Czech, 2023, 68(3):106-115 | DOI: 10.17221/79/2022-VETMED


Porcine deltacoronavirus (PDCoV) and porcine sapelovirus (PSV) are two viruses that can cause diarrhoea in pigs and bring great economic loss to the pig industry. In this research, a duplex real-time quantitative polymerase chain reaction (qPCR) assay based on SYBR Green І was developed to simultaneously detect PDCoV and PSV. No specific melting peaks were found in other porcine diarrhoea-associated viruses, indicating that the method developed in this study had good specificity. The detection limits of PDCoV and PSV were 1.0 × 101 copies μl–1 and 1.0 × 102 copies μl–1, respectively. The duplex real-time qPCR assay tested two hundred and three (203) intestinal and faecal samples collected from diarrhoeal and asymptomatic pigs. The positive rates of PDCoV and PSV were 20.2% and 23.2%, respectively. The co-infection rate of PDCoV and PSV was 13.8%. To evaluate the accuracy of the developed method, conventional PCR and singular TaqMan real-time qPCR assays for PDCoV/PSV were also used to detect the samples. The results showed that the duplex real-time qPCR assay was consistent with the singular assays, but its sensitivity was higher than conventional PCR methods. This duplex real-time qPCR assay provides a rapid, sensitive and reliable method in a clinic to simultaneously detect PDCoV and PSV.

Recent advances of using organic acids and essential oils as in-feed antibiotic alternative in poultry feedsReview

Nguyen Hoang Qui

Czech J. Anim. Sci., 2023, 68(4):141-160 | DOI: 10.17221/99/2022-CJAS


Antibiotics used in animal feeds have recently been strictly regulated to avoid antibiotic resistance in humans. Thus, scientists are compelled to find new feed additives to replace antibiotics in feed. Apart from having a zero effect on human health, new alternatives may even be able to further improve production performance. Essential oils (EOs) and organic acids (OAs) stood out as powerful and useful replacements for both animal production and human population. These are added to chicken diets and drinking water to induce a favourable growth response. Hence, enhancing the nutrient digestibility, performance, and immunity of birds, OAs and EOs are used in livestock production mainly to inhibit the growth of harmful bacteria and simultaneously maintain the balance of intestinal bacteria. This improves the digestion of nutrients and mineral absorption which will eventually lead to better feed efficiency. The addition of OAs and EOs also causes the intestinal lining to thin, which further enhances nutrient absorption and utilization. Not only for poultry production, the effect of OAs and EOs can also improve poultry immunity and antioxidant capacity. However, the effects will vary depending on the type of OAs and EOs because their mode of action is dependent on their pKa value. In the present review, beneficial properties of OAs and EOs, as well as various dose combinations, to promote their optimal use in poultry nutrition and production will be examined. Their effects on supporting protein digestion, faster absorption of minerals, especially microminerals, stimulating growth performance, regulating antioxidant capacity, and improving immune response will be explored.

Correlation between acrylamide content and colour in some baked productsOriginal Paper

Zana Mohammed Abdulazeez, Abdel Moniem Ibrahim Mustafa, Fehmi Yazici

Czech J. Food Sci., 2023, 41(2):137-143 | DOI: 10.17221/244/2022-CJFS

The objective of this study was to quantify the concentration of acrylamide in biscuits, bread, and cake obtained from industrial and local bakeries, classified based on their colour intensity as light, medium, or dark. The analysis was performed using gas chromatography-mass spectrometry (GC-MS). Sugar, moisture, and asparagine analyses were also carried out simultaneously. The results demonstrated that the acrylamide content in biscuits, bread, and cakes varied according to the colour (light 109.9 ± 7.95, 214.7 ± 27.40, and 128.6 ± 7.05; medium 176.3 ± 15.16, 387.0 ± 87.71, and 804.3 ± 17.16; and dark 407.6 ± 105.13, 555.8 ± 16.20, and 1 015.0 ± 83.68 µg·kg–1, respectively). Statistically, significant differences were observed between acrylamide content and product colour density (P < 0.05). It can be concluded that the acrylamide content increases as the product colour intensity rises due to the increased baking temperature at which the reaction between reducing sugars and asparagine takes place, resulting in the formation of acrylamide. The results suggest that selecting commercial bakery products based on colour may be beneficial for reducing the daily intake of acrylamide by consumers. Therefore, it is recommended to avoid dark-coloured bakery products.

Effects of fungicides and antagonistic marine-derived fungi on rice seedling promotion and rice sheath blight controlOriginal Paper

Tida Dethoup, Rotchana Klaram, Arom Jantasorn

Plant Protect. Sci., 2023, 59(2):159-173 | DOI: 10.17221/95/2022-PPS


Fourteen fungicides and three marine-derived fungi were tested for their activities in the promotion of rice seedling development and their efficacy in controlling rice sheath blight disease in two rice varieties, RD43 and KMDL105, under greenhouse conditions. The results showed that the rice seeds treated with carbendazim 50%SC and azoxystrobin 25%SC displayed the best promotion of rice growth in root length and shoot height at seven and 14 days in rice var. RD43. Rice seeds from var. KMDL105 treated with thiophanate-methyl 70%WP exhibited significant stimulation of rice seedling growth resulting in the greatest increase in root length and shoot height at seven and 14 days. In pot experiments with rice var. RD43, spraying with triforine 19%EC, propiconazole 25%EC, trifloxystrobin 50%WG, and validamycin 3%SL showed a high level of fungicidal activity in controlling sheath blight disease, with this evidenced by significant suppression of the disease severity by 71–75% and 61–73% at seven and 14 days after inoculation, respectively. The treatment of rice var. KMDL105 with validamycin 3%SL exhibited the best activity in controlling sheath blight disease by reducing disease severity by up to 62% and 64% at seven and 14 days, respectively. The antagonistic marine fungi tested in this study displayed low activity against sheath blight disease and low effects on rice seedling promotion compared to fungicides. Specifically, Trichoderma harzianum KUFA 0689 and T. asperellum KUFA 0677 reduced sheath blight disease severity by 42–46% and 27–37% at seven and 14 days, respectively.

Allometric coefficient in broilers and development of white striping and wooden breast myopathiesOriginal Paper

Fernando Alberto Benitez dos Santos, Claudia Marie Komiyama, Vivian Aparecida Rios de Castilho Heiss, Maria Fernanda Castro Burbarelli, Rodrigo Garófallo Garcia, Deivid Kelly Barbosa, Bruna Barreto Przybulinski, Leonardo de Oliveira Seno, Andrey Sávio de Almeida Assunção, Erika Rosendo de Sena Gandra

Czech J. Anim. Sci., 2023, 68(5):212-221 | DOI: 10.17221/182/2022-CJAS


The aim was to study the evolution and severity of white striping and wooden breast myopathies and to carry out a study on the parameters of meat quality and allometric growth of male and female broilers at different ages. The experiment was conducted in a broiler house in a completely randomized design, using 960 one-day-old chicks of the Cobb® strain, with 480 chicks of each sex. 96 birds per treatment were slaughtered weekly at the age of 21 to 49 days. The present study analyses the occurrence of myopathies, their scores, and their evolution over the age of the birds, comparing males and females. Further assessments included the allometric coefficient and meat quality analyses such as physical measurements (breast length, width, and thickness), pH, colour (L*, a*, and b*), drip loss, water-holding capacity, cooking loss, and shear force. The results demonstrate that the sex of birds did not affect the occurrence of white striping and wooden breast myopathies. However, the degree of myopathies evolved with advancing age, suggesting the weight as a predisposing factor for these myopathies. The emergence of white striping myopathy occurred as early as in the third week of the birds’ life. Wooden breast did not develop as isolated myopathy, occurring only in the concomitant presence of white striping. The allometric coefficient demonstrated that regardless of the sex and age, broiler breast had positive heterogonic growth, i.e. late growth in relation to the other parts of the body.

Contrasting tillage systems and vertical arable layer stratification impacts on soil aggregates and root biomass in LithuaniaOriginal Paper

Inga Andru¹kaité, Vaclovas Bogu¾as

Res. Agr. Eng., 2023, 69(2):76-84 | DOI: 10.17221/29/2022-RAE

Tillage intensity can affect soil quality through soil aggregates, which are strongly associated with organic carbon. In this study, we evaluated the effect of different intensity tillage (conventional ploughing (CP), shallow ploughing (SP), deep cultivation (DC), shallow cultivation (SC), no-tillage (NT)) on soil organic carbon (SOC) and soil aggregates at the 0-5, 5-10, 10-20 and 20-30 cm layers and root biomass at the 0-10, 10-20 and 20-30 cm layers. The field experiment on spring barley (Hordeum vulgare L.) consisted of a split-plot arrangement with four replications. Dry soil aggregates were evaluated using the mean weight diameter (MWD) index. Wet aggregates were assessed using a water-stable aggregate (WSA) index. We identified that tillage intensity significantly influenced the MWD index at four soil levels. Shallow tillage showed greater results at 0-5 and 5-10 cm depths. However, deep tillage increased the MWD at 10-20 and 20-30 cm depth. NT was dominated by evaluating the WSA at every level of the soil. SOC was correlated with WSA. The highest SOC was found under NT. The different tillage intensities did not significantly affect root biomass.

Effect of biochar or biochar and urea supplementation on feed intake, milk yield, feed conversion and methane production of dairy cowsOriginal Paper

Georg Terler, Manuel Winter, Michael Mandl, Joseph Sweeney, Andreas Steinwidder

Czech J. Anim. Sci., 2023, 68(6):245-254 | DOI: 10.17221/38/2023-CJAS


Feed additives belong to a number of climate change mitigation strategies being sought to reduce methane emissions in ruminants. In this study, the effect of biochar or biochar and urea supplementation on dairy cow performance and methane production was assessed. Eighteen cows were used in a 3 × 3 latin square design with three feeding groups: control with no supplementation (CO), biochar supplementation (BC, 200 g/day) and biochar and urea supplementation (BC + U, 200 g/day biochar and 90 g/day urea). All cows were fed a forage mixture ad libitum and 5 kg of concentrates per day on average. Methane emissions were measured in respiration chambers. Biochar as well as biochar and urea supplementation did not affect total dry matter, energy and utilisable protein intake. However, lignin intake was higher in the BC group and crude protein intake was higher in the BC + U group compared to the CO group. Supplementation of feed additives did not affect milk production and milk composition, except for the higher milk urea content in the BC + U group. Feed conversion, diet digestibility and methane production were not affected by feeding strategy. In conclusion, biochar supplementation does not reduce methane emissions, but it does not negatively affect dairy cow performance.

Effect of silicon dioxide application and potassium levels on morphophysiological properties and storable seed yield of hybrid super sweet maize (Zea mays L. 'Elika') under drought stressOriginal Paper

Seyed Gholam Reza Hosseini, Behzad Sani, Hamid Mozafari, Hamidreza Zabihi, Faezeh Rajabzadeh

Plant Soil Environ., 2023, 69(7):324-332 | DOI: 10.17221/302/2022-PSE


In this experiment, we investigated the effects of drought combined with exogenous silicon (Si) and potassium application on super sweet corn growth and development. Drought stress caused decreases in the stem diameter, leaf area, cob length, cob diameter, 100 seed weight, seed number, cob yield, biologic yield, and relative water content (RWC), but proline content and catalase activity were higher under drought stress conditions. The results of a two-year experiment showed that potassium sulfate application and foliar application significantly increased RWC in drought stress conditions, and the highest increase was related to treatment with potassium sulfate in an amount of 25 kg/ha. Under normal irrigation conditions, with 25 kg of potassium sulfate per ha and Si foliar application, the maximum cob diameter (5.85 cm) was observed. Si application did not significantly affect proline content under normal irrigation conditions but increased proline content under drought stress. The highest proline content (10.77 mmol/g fresh weight) was recorded in the Si application using 25 kg of potassium sulfate per ha under drought stress conditions. Also, applying potassium sulfate with silicone foliar spraying had no significant effect on biologic yield under normal irrigation conditions. However, under drought stress treatments, biologic yield increased by applying 15 and 25 kg/ha of potassium sulfate and Si foliar spraying. In summary, applying potassium sulfate and exogenous Si can enhance the antioxidant system of the plant, promote the RWC, thus improving biologic and cob yield, and enhance the drought resistance of super sweet corn.

Forest cover change detection using Normalized Difference Vegetation Index in the Oued Bouhamdane watershed, Algeria – A case studyOriginal Paper

Boubaker Khallef, Rabah Zennir

J. For. Sci., 2023, 69(6):254-265 | DOI: 10.17221/192/2022-JFS

The Algeria forest, particularly in the northeastern region, has undergone profound changes in recent years. The Oued Bouhamdane watershed has a great forest potential, which is threatened by several factors of natural and human origin, resulting in a decrease in forest cover. It requires adequate forest monitoring to support the sustainable forest management of this watershed, which is possible thanks to satellite imagery. The objective of this research is to study the spatiotemporal dynamics of the vegetation cover of the Oued Bouhamdane watershed between 2013 and 2022 using remote sensing data. This study is based on the use of Landsat 8 and 9 images for two dates in 2013 and 2022, and the calculation of the Normalized Difference Vegetation Index (NDVI) to identify changes in vegetation cover between 2013 and 2022. The calculation of NDVI and the realization of the vegetation change map showed a regression of the forest cover between 2013 and 2022 with a rate of –5.53% of the total of the study area with a general negative change of 28.62% of the study area. This regression is essentially linked to natural and anthropogenic factors. This work can be a valuable tool for sustainable management of the forest of this watershed; moreover, the method is also adaptable to other watersheds of the northeastern region of Algeria.

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