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Because of the COVID-19 pandemic and the war in Ukraine, agricultural commodities had significant price increases, which inevitably implies high risk. In this article, we try to mitigate the extreme risk of corn and soybeans by constructing multivariate portfolios with developed and emerging European stock indices. We measured extreme risk via conditional value at risk. To address different goals that investors might prefer, we produced portfolios with the lowest risk and highest return-to-risk ratio. According to the results, corn and soybeans had relatively high portfolio shares. However, they are the riskiest assets because they have a very low pairwise correlation with the stock indices. Portfolios with emerging European indices had better risk-reducing results, considering both agricultural commodities because these indices are less risky than developed indices. In particular, the risk reductions of corn were 38% and 50% in the portfolios with developed and emerging stock indices, respectively, whereas, for soybeans, the results were 28% and 41%, respectively. In optimal portfolios, emerging European stock indices had the upper hand in most cases.

The black soldier fly (Hermetia illucens) serves as an alternative source of protein in poultry production. However, there is little available information on feeding live black soldier fly larvae (LBSFL) to hens. The present study filled this gap by testing the effect of dietary LBSFL on laying hen performance, feed economy, egg quality, and yolk fatty acid composition. In total, one hundred forty-four 25-week-old Charoen Pokphand Brown laying hens were assigned to four groups. Experimental birds were provided a basal diet supplemented with 0.0 (T1), 10.0 (T2), 20.0 (T3), or 30.0 (T4) g/kg LBSFL. Our results indicated that no effects on body weight gain, egg yield, egg weight, or egg mass (P > 0.05) were observed in any of the dietary LBSFL groups. The addition of 10.0 g/kg LBSFL decreased feed intake, feed conversion ratio, and feed cost (P < 0.05), contrary to two higher doses of LBSFL. Similarly, the egg quality parameters were not influenced (P > 0.05) by the dietary LBSFL except for the egg yolk colour which significantly decreased with increasing LBSFL levels. Furthermore, the values for lightness (L*), redness (a*), and yellowness (b*) of the egg yolk were different (P < 0.05) between the four treatments. Paler egg yolk was found in the 20.0 and 30.0 g/kg LBSFL groups compared to the control group (P < 0.05). Significantly lower deposition of C18:3n6 was found for 30.0 g/kg, whereas there was a significant decrease in deposition of C22:6n3 with all LBSFL groups (P < 0.05). C20:3n6 levels, on the other hand, increased significantly in the 20.0 g/kg LBSFL group (P < 0.05). Thus, feeding LBSFL has no potential to increase the proportion of anti-inflammatory n-3 fatty acids. From an economic point of view, it is advantageous to feed the hens a dose of 10 g/kg LBSFL.

Listeria monocytogenes is not only a pathogen causing a serious food-borne disease in humans but can also occur as a saprophyte in the natural environment. This study aimed to evaluate the occurrence of L. monocytogenes obtained from the natural environment of the Czech Republic (hereinafter Czechia) and Slovakia in 2016–2018 and to compare the clonal relationship of strains circulating in the environment with the strains originating from the food chain and humans. Altogether, 217 samples of mud, surface water, vegetation and soil were collected in 61 locations. Samples were processed according to the modified EN ISO 11290-1 standard. The obtained L. monocytogenes isolates were characterised using serotyping, macrorestriction analysis, followed by pulsed-field gel electrophoresis, whole genome sequencing (WGS), and antimicrobial susceptibility testing. L. monocytogenes were detected in 8.8% of the examined samples and were isolated in 15 locations, mainly from the mud from the banks of the surface water sources. Altogether, 25 L. monocytogenes strains were obtained from 19 positive samples. Serotypes 1/2a, 4b, and 1/2b were detected among the strains. Twenty combined AscI/ApaI pulsotypes were obtained by macrorestriction analysis. Altogether, 12 sequence types (STs) were detected using Multi-locus sequence typing (MLST) by WGS, with ST451 being the most frequent. The core genome MLST analysis revealed a heterogeneous population of environmental strains. No phenotype resistance was detected by antimicrobial susceptibility testing. Screening of antimicrobial-resistance genes using the platform ResFinder revealed the genes fosX in 24 isolates and blaTEM-116 in one isolate. The occurrence of L. monocytogenes in various samples from natural environments within wide altitude range during different seasons of the year may highlight this bacterium's remarkable adaptability and exceptional tolerance to external factors. Serotype distribution of the strains circulating in the natural environment of Czechia and Slovakia seems to reflect distribution in the human population more than in the food chain.

This work aims to separate antimicrobial lipopeptides from fermentation by Aureobasidium pullulans PA-2, and verify its antimicrobial activity and the optimum condition of lipopeptide production. Using high performance liquid chromatography (HPLC) analysis, the lipopeptides with antimicrobial activity was evaluated with the agar well diffusion test. Response surface methodology (RSM) was used to determine optimum conditions for lipopeptides from A. pullulans PA-2. The lipopeptides with antimicrobial activity in the fermentation supernatant of the PA-2 strain was found to be the Aureobasidin A (AbA). In vitro antagonistic tests showed that the minimal inhibitory concentration (MIC) of AbA against Staphylococcus aureus and Escherichia coli was 0.5 and 1.0 mg/mL, respectively. The optimal fermentation conditions were: inoculum size 6.8 % (v/v)(OD₆₀₀=0.25), rotation speed 216 rpm, culture temperature 26 ℃, liquid volume 125 mL and initial pH7. Under this condition, predicting yield of the antimicrobial lipopeptides by the model was 940 mg/L, observed yield 920 mg/L, which was 51 % more than that of before optimization (610 mg/L).

Tail biting in pigs represents a very serious problem in modern pig farming, particularly with the intensification of the industry. It is considered a multifactorial syndrome and can be caused by various factors, leading to significant economic losses through reduced weight gain and partial or total condemnation of slaughtered carcasses due to secondary bacterial infections. The aim of this article is to summarise the current knowledge regarding tail biting in pigs, with a primary focus on the use of slaughterhouses for evaluating tail lesions and monitoring their prevalence. The introduction addresses the factors influencing the incidence of tail biting in pig farms and prevention strategies. Subsequent sections cover topics such as tail docking, the negative effects of pig tail biting, the advantages and drawbacks of examining tail lesions in slaughterhouses, and the methodical procedure for evaluating such lesions. Additionally, the article discusses the relationship between tail lesions and meat inspection findings, as well as the prevalence of tail lesions in various European countries.

One of the main causes of having low crop efficiency in Peru is the poor management of water resources; which is why the main objective of this article is to estimate the amount of irrigation water required in quinoa crops through a comparison between the machine learning and AquaCrop models. For the development of this study, meteorological data from the province of Jauja and descriptive data of quinoa crops were processed and a simulation period was established from June to December 2020. From the simulation carried out, it was determined that the best model to predict the required irrigation water is the Adaptive Boosting (AdaBoost) model in which it was observed that the mean and standard deviation of the AdaBoost models (mean = 19.681 and SD = 4.665) behave similarly to AquaCrop (mean = 19.838 and SD = 5.04). In addition, the result of ANOVA was that the AdaBoost model has the best P-value indicator with a value of 0.962 and a smaller margin of error in relation to the mean absolute error (MAE) indicator with a value of 0.629. Likewise, it was identified that, for the simulation period of 190 days, 472.35 mm of water was required to carry out the irrigation process in red quinoa crops.

This study was conducted to investigate the resistance of 4 wild eggplant species (Solanum aethiopium group Aculeatum, S. incanum, S. macrocarpon, S. linnaeanum), 3 cultivated eggplant varieties (‘Anamur F1’, ‘Pala’, ‘Yamula’), 1 eggplant rootstock (Köksal F1) and 34 interspecific hybrids to Verticillium dahliae Kleb. and Fusarium oxysporum f.sp. melongenae. Disease resistance of eggplant genotypes was determined by the pathogenicity test. The disease severity values varied between 0–80% for Verticillium and between 0–100% for Fusarium. Among the 42 genotypes, 18 genotypes were found to be moderately resistant and 1 genotype was found to be highly resistant to Verticillium. At the same time, 2 of the 42 genotypes were found to be moderately resistant and 22 of the 42 genotypes were found to be highly resistant to Fusarium. All hybrids with S. integrifollium, Solanum aethiopicum group Gilo as father were found to be highly resistant to Fusarium oxysporum f.sp. melongenae. Solanum linnaeanum did not exhibit any disease symptoms and was found to be highly resistant to both disease agents. Present interspecific hybrid eggplant genotypes with known resistance to Verticillium and Fusarium wilt are expected to have significant contributions in developing new eggplant rootstocks and hybrid eggplant cultivars in the future.

Sorghum is an important crop, and starch and phenolic compounds are major and important components in the sorghum grain. However, the underlying critical genetic elements contributing to the rich portfolio of nutrients in sorghum grains are largely unknown. Transcriptomic methods were employed to characterize the expression patterns at five different grain developmental stages of Hongyingzi (an important brewing sorghum), and another two grain sorghums, Jinuoliang 1 and Hongliangfeng 1, for comparison. The uniquely expressed genes were identified at each developmental stage of Hongyingzi when compared with the other two sorghum cultivars. The co-regulated genes at different developmental stages and the regulatory network were determined; the determinant genes and single-nucleotide polymorphisms located at the promoters of these genes involved in starch and phenolic compounds biosynthetic pathways were also identified. These results will provide insights into the potential regulatory network and further contribute to the clarification of the key determinant genes involved in the biosyntheses of starch and phenolic compounds. Meanwhile, some new transcripts and genes were identified at five different developmental stages of grains of the three sorghum cultivars. Our work can provide impetus for further study of the genes responsible for the biosynthesis of starch and phenolic compounds in the sorghum grain, and pave a way for functional validation of a batch of potential genes and single-nucleotide polymorphisms proposed in current work.

This study is to explore the effects of natural tannin (NBT) on intestinal injury in piglets challenged with enterotoxigenic Escherichia coli (ETEC). Thirty-two 7-day-old piglets were divided into four groups: I) control group, piglets were fed a basic diet without challenge; II) ETEC group, piglets were fed a basic diet and challenged with enterotoxigenic E. coli; III) NBT + ETEC group, piglets were fed a basic diet with supplementation of 2 g/kg NBT and challenged with enterotoxigenic E. coli; and IV) ZnO + ETEC group, piglets were fed a basic diet with supplementation of 3 g/kg ZnO and challenged with enterotoxigenic E. coli. Results showed that diarrhoea rate was significantly increased in ETEC group, whereas it was decreased after NBT supplementation (P < 0.05); NBT supplementation significantly increased the average daily gain of ETEC-infected piglets. ETEC group showed damaged intestinal morphology (as indicated by decreased villus height, surface area and increased crypt depth) and barrier function (as indicated by decreased d-xylose content and increased diamine oxidase activity), induced oxidative stress (as indicated by increased myeloperoxidase activity) while supplementation of NBT had an obvious impact on the recovery of intestinal function and alleviated the oxidative damage. Further analysis showed that NBT could decrease the expression of genes related to intestinal injury (matrix metalloproteinase-3), and specifically upregulate the expression of oxidative stress-related genes (nuclear factor erythroid 2-related factor 2 and hypoxia inducible factor-1). Overall, dietary supplementation of NBT relieved intestinal injury and oxidative stress in piglets challenged with ETEC. NBT could be an alternative to ZnO as a feed additive in piglet diet.

Orange-fleshed sweetpotato (OFSP) is a sustainable and inexpensive source of vitamin A that contributes to food and nutritional security in developing countries. Its adoption is low due to unconformity of the variety with community food culture, limited cultivar selection and breeding studies. The current study aimed at breeding of promising OFSP genotypes in Benin using three parental lines. Two hundred and seventy-four seeds and one hundred and six genotypes were obtained through biparental crosses of Tio-joe (imported orange-fleshed variety), Vobodouaho (local white-fleshed variety) and ACAB220 (local orange-fleshed variety). The phenotypic diversity of genotypes was assessed using 10 leaf characters and storage root flesh colour through multiple correspondence analysis and hierarchical cluster analysis. The results indicated cross-compatibility between Tio-joe and ACAB220 when ACAB220 was used as female. Reciprocal cross-compatibility was found between ACAB220 and Vobodouaho. The genotypes could be separated into two main populations and classified into five cluster groups. Promising intermediate and pale orange genotypes were obtained indicating possibilities to incorporate beta-carotene into the white background of the Vobodouaho variety through further backcrossing.

This research scrutinised the opportunity of upcycling waste from the food industry by extracting dietary fibre from purple aubergine. One of the challenges addressed was enzymatic browning, which negatively influenced the quality of the fibre extracted from fresh aubergine. Various pre-treatment procedures were assessed, including using citric acid, sulphite immersion, peeling and thermal processing, to determine their effects on extraction yield, colour, and the functionality of the resultant dietary fibre. Findings indicated that a pre-treatment method involving aubergine peeling, sulphite solution immersion, and subsequent steaming before extraction produced optimal results, enhancing both whiteness index and water-holding capacity. Experiments were conducted with traditional enzymes supplemented with cellulase, xylanase, and lipase for enzymatic extraction. The addition of lipase notably elevated the extraction yield and water-holding capacity, albeit with an undesired darkening effect on the dietary fibre. In contrast, the application of xylanase emerged as the most effective treatment, delivering the highest overall quality for the derived dietary fibre.

We investigated the compatibility between adzuki bean and Bradyrhizobium USDA strains and the relation between the genetic diversity of indigenous adzuki bean-nodulating bradyrhizobia and their geographical distribution in Japan. Six Bradyrhizobium USDA strains were used in the inoculation test, and B. elkanii USDA 94 showed higher symbiotic efficiency than other strains. Two adzuki bean cultivars were used to isolate indigenous adzuki bean-nodulating bradyrhizobia. Their genetic diversity and community structure were analyzed by restriction fragment length polymorphisms of PCR amplicons to target the 16S-23S rRNA gene internal transcribed spacer region, using 11 USDA Bradyrhizobium strains as reference strains. Furthermore, we performed diversity analysis, non-metric multidimensional scaling analysis based on the Chao index, and polar ordination analysis to explain the relation between community structure and geographical distribution of the adzuki bean-nodulating bradyrhizobia. The major clusters were Bj123 in Hokkaido and Kyoto, Bd110 in Fukushima and Shimane A, Be76 in Shimane B and Fukuoka, Bj6 in Miyazaki, and Bj6 and BeOK in Okinawa. Japan's indigenous adzuki bean-nodulating bradyrhizobial community was strongly and significantly correlated with latitude, longitude, annual mean temperature, and electrical conductivity. This result suggests that the adzuki bean-nodulating bradyrhizobial community is influenced by multiple environmental factors.

Jejunal haemorrhage syndrome (JHS) is a sporadic and fatal enterotoxaemic disease in dairy cows associated with acute development and poor prognosis despite treatment. A 5-year-old Holstein cow with no reported pregnancy, three calving numbers, and 303 days in milk presented with hypothermia, discomfort, and inappetence. Anaemia, dehydration, faeces with blood clots, and absence of rumen and bowel movements were observed. We identified the presence of neutrophilia, hyperglycaemia, hypoproteinaemia, azotaemia, hyperlactatemia, hypocalcaemia, hypermagnesemia, hypokalaemia, and hypochloraemia through blood analyses. Necropsy and histopathologic examination revealed a dilated bluish-purple jejunum, blood clots within the jejunum, neutrophil infiltration into the submucosa of the jejunum, and vascular necrosis. Retrospective examination revealed extraordinary patterns of rumination time, activity, rumen mobility, and rumen temperature using biosensors and decreased milk yield. The abnormalities in the affected cow were detected before recognition by farm workers. To the best of our knowledge, this is the first report to examine data from biosensors in a cow with JHS. Our findings suggest that using biometric data may help understand the development of JHS.

This paper is focused on the description of the black mulberry (Morus nigra), its ecology and the possibilities of its in vitro propagation for quick and efficient obtaining of a large quantity of clones in a relatively short time for subsequent planting or sale. Due to ongoing climate change, it is considerable to use mulberry trees in horticulture, agroforestry and forestry under the conditions of Central Europe. The use of the mulberry is conditioned by the availability of planting stock. A proven and successful method of mulberry propagation is in vitro cultivation. Based on literature review, the recommended composition of planting media and other procedures for in vitro cultivation of mulberries are presented. The aim of the article is to inform foresters about the possibilities of using the black mulberry tree in our conditions and, using its example, to point out the possibility of using non-standard species of trees both as part of adaptation measures to the expected climate change and as one of the options for increasing the biodiversity of the landscape.

Centaurea cyanus is an annual weed mostly infesting winter cereals and rape. The aim of the study was to provide insights into the association between the seed germination characteristics and the herbicide sensitivity in C. cyanus in the presence of the genetic background control. Four populations of this species resistant to acetolactate synthase (ALS) inhibitors were tested. A germination study was conducted in a growth cabinet. Plants were sprayed at the 2- to 3-leaf stage with a field dose of florasulam (5 g/ha). There were four herbicide treatments dates, which included plants that germinated up to the seventh day, between eight and 12, 13 and 15, 16 and 20 days of the germination study. The germination dynamics of the four tested populations of C. cyanus resistant to florasulam was diversified. Three of them reached their maximum germination on the fourth day after sowing, however, the germination of the fourth population was spread over time with the highest number of germinated seeds found seven and twelve days after sowing. The germination time of the plants belonging to the resistant C. cyanus populations differentiated their reaction to florasulam. The conducted study indicated that the germination biology of ALS inhibitor-resistant populations of C. cyanus is diverse, which makes it difficult to introduce universal management strategies of this species into agricultural practice. Integration of control methods is recommended, including delaying the crop sowing date.

A 5-year-old intact female Shih Tzu was presented with acute onset of hind leg paralysis. The neurologic examination revealed severe T3-L3 myelopathy. The differential diagnoses included degenerative, anomalous, traumatic, inflammatory, vascular, metabolic, and neoplastic changes. The results of the paraclinical examinations and diagnostic imaging narrowed the list of differential diagnoses and, along with the patient’s deteriorating condition, led to the owner’s decision to euthanise the dog. The histologic findings of the spinal cord specimens indicated a tumour of the blood vessels formed by the proliferation of endothelial cells, which may present as either capillary or cavernous structures. In this case, the tumour was a capillary-type haemangiosarcoma. The primary site of proliferation could not be determined in this case because no mass formation was noted while performing the necropsy.

Long-term field experiments provide a valuable dataset for predicting changes in soil organic carbon (SOC) stocks in different agricultural systems. The RothC-26.3 model was used to simulate changes in SOC in the monoculture of spring barley (Hordeum vulgare L.) and the Norfolk crop rotation during 1972–2100. The potential of the Gleyic Fluvisol Clayic to sequester organic carbon was investigated. The studied soil was heavily textured, with medium organic carbon content. Four management scenarios in the monoculture and six management scenarios in the Norfolk crop rotation were evaluated. Three different global climate models (MPI, MRI, CMSS) representing the uncertainty of future climate conditions were used. Results showed that carbon stocks were mainly influenced by plant residue inputs and exogenous organic materials application. The projection showed trends of carbon stocks decreasing in the case of monoculture management. Results also documented that management scenario D with straw incorporation and intercrops represented sustainability and carbon stock increase during all modelled climate scenarios. The SOC stock at the end of the century was approximately 66 t/ha. This represents a moderate sequestration of SOC of approximately 0.09 t/ha/year.

The present study aimed to investigate the effect of catalase (CAT) on the growth performance, antioxidation, and microbial metabolism of weaned rabbits. Dietary treatments consisted of control and CAT supplementation at 100 (T1), 150 (T2), and 200 IU/kg of diet (T3). A total of 240 weaned rabbits were randomly assigned to 4 groups with 6 replicates of 10 rabbits each. The feeding trial lasted for 28 days. Results showed that T2 and T3 increased (P < 0.05) body weight gain and gain/feed ratio, and decreased (P < 0.05) diarrhoea rate, compared to the control. Also, serum antioxidative parameters and oxidative stress products were beneficially regulated (P < 0.05) by the dietary CAT administration. Faecal microbiota including Bacteroidetes, Prevotella, and Bifidobacterium in T2 or T3 was increased (P < 0.05). Dietary CAT with changed microbiota further influenced the metabolites from carbohydrates and proteins, evidenced by increased lactic acid, acetate, branched-chain fatty acids, and short-chain fatty acids, and decreased valerate, isovalerate, methylamine, tryptamine, putrescine, cadaverine, spermidine, and total amines. It is concluded that CAT can be used to improve growth performance by beneficially regulating the antioxidation, microbiota, and metabolites in weaned rabbits.

Chicken eggshells are a source of calcium and protein that can be added to restructured meat products, including patties. This study aimed to improve the chemical quality of beef patties with the addition of chicken eggshell nanopowder, including pH, protein content, ash content, fat content, antioxidant activity, calcium, microstructural quality, and identification of amino acid profiles. The method used in this study was a laboratory experiment arranged in a completely randomised design with five treatments: without the addition of chicken eggshell nanopowder as a control, the addition of chicken eggshell nanopowder 0.1, 0.3, 0.5, and 0.7% (w/w) with five replications. Data were analysed using the analysis of variance. It was followed by Duncan's multi-range test when the differences were significant or very significant. The research results show that the addition of chicken eggshell nanopowder to beef patties had a very significant effect (P < 0.01%) on pH, protein content, ash content, fat content, antioxidant activity, calcium, microstructural quality through scanning electron microscopy, and several amino acids. The addition of 0.7% chicken eggshell nanopowder could improve the chemical quality of the beef patties. The patty matrix was uniform and compact, and the air voids were getting tighter with the addition of chicken eggshell nanopowder, which was increasing based on scanning electron microscopy observations. Moreover, beef patties had various types of amino acids to achieve the best treatment.

Rf(fa), a new restoring fertility gene in rice, was previously located to a large region on Chromosome 10. The large number of genes within the region made cloning of Rf(fa) difficult. To perform the cloning and further elucidate the molecular mechanism, we reconstructed a mapping segregation population (BC₁F₁) of 12 000 plants. Using the population and polymorphism of simple sequence repeat (SSR) molecular markers, we finally mapped Rf(fa) between the two SSR molecular markers MM2000 and RM25658, within a 78.87 kb region. By de novo sequencing of a restoring line of CMS-FA hybrid rice, we obtained the genomic sequence of the mapping region, which provided the basis for the prediction of the candidate gene(s) of the target gene and for the comparison of genomic sequence differences between wild and cultivated rice. Within the mapping region, the genomic sequence of the wild rice was significantly different from that of cultivated rice. There were ten genes in the final mapping region. A pentatricopeptide repeat (PPR) protein gene was predicted as the candidate gene of Rf(fa). Our results laid a solid foundation for the final cloning and molecular mechanism analysis of the gene. The identified molecular markers tightly linked to Rf(fa) will facilitate the marker assisted selection in breeding of CMS-FA hybrid rice.

Designing and constructing a road network is one of the most critical steps of the development process in managing natural resources. The current research is going to investigate the application of network connectivity indices to the analysis of road networks in the forested watershed. First, the road network suitability map (RNSM) was created to emphasize the technical and physiographic criteria and integrated management scenarios using a weighted linear combination (WLC) and analytic network process (ANP). Subsequently, three road network alternatives (RNA) were assigned based on the priorities in the RNSM. In order to try to determine the appropriate alternative, the network connectivity of the designed alternatives looked into the forest and non-forest land uses, as well as the whole study area, using the values of alpha (α), beta (β), gamma (γ), eta (η), network density (ND) and detour indices (DI) in the context of the graph theory. Results show that the road density of the RNA2 variant (11.56 m·ha^–1) is shorter than the other alternatives and the existing road network (ERN). In addition, dealing with the whole study area, we realized that the index values which are related to the number two reflect a better status than the other alternatives of alpha, beta, gamma, eta and detour index, in which they were identified to be 0.44, 1.34, 1.16, 0.45 and 0.83, respectively. RNA2 is chosen as the appropriate road network according to the network connectivity, technical and physiographical criteria, along with integrated management scenarios. Further control measures and field surveys are recommended to achieve more relevant results.

The present study aimed to determine the incidence and coincidence of white striping (WS), woody breast (WB) and spaghetti meat (SM) in raw chicken breast meat (n = 300) collected 3–3.5 h post mortem from commercial broiler flocks (Ross 708 males, 56 days of age). The fillets were scored for the occurrence and severity of WS, WB and SM using visual and tactile methods, and the relationship between myopathies and breast weight was evaluated. A total of 88% of samples (out of 300) had either one or a combination of three myopathies, leaving only 12% without any myopathies. Approximately 29% of the fillets had only one myopathy present, with 59% of the samples demonstrating the cooccurrence of breast myopathies. In the present study, 47.7% of WB samples also exhibited WS, while the cooccurrence of WB and SM was exhibited in 14.7%. Based on binomial logistic regression, an increase in WB scores decreases the odds that the fillet may be affected by SM myopathy. No association was found between SM and fillet weight. Multinomial logistic regression showed that the weight of breast fillets was positively associated with WB1 (P = 0.011), WB2 (P < 0.001), and WB3 (P < 0.001). Furthermore, positive associations were found between WS1 and WB3 (P = 0.004) as well as between WS2 and WB3 (P < 0.001). The percentage distribution changes of each WB and WS myopathy score were used in relation to the 10 weight groups. The percentages of WB3 increased and WB0 decreased as the average fillet weight increased. Regarding linear regression, these results exhibited a positive linear relationship for both WB (R² = 0.91, P < 0.001) and WS (R² = 0.71, P = 0.002) myopathy with fillet weight. A high prevalence of myopathies was observed. The incidence of severe WB categories and the overall high cooccurrence rate are alarming.

The information on the behaviour of potassium (K+) in tropical paddy rice soils, which is important for a better understanding of the plant availability of K+ is still very limited. We compared the quantity-intensity (Q/I) relationships for K+ under conventional tillage and no-tillage systems in tropical paddy fields in the absence and presence of K+ fertiliser in the addition of nitrogen. The results showed that the values of the activity ratio for K (ARK) and potential buffering capacities (PBCK) in the no-tillage rice field were respectively 16% and 33% higher than that in the conventional tillage field. With the addition of K fertiliser, the value of exchangeable K in equilibrium (ΔK0) in the no-tillage paddy field was 67.9% greater than that in the conventional tillage field. This indicates that K fertilisation is more efficient when applied on a no-tillage paddy field. When the K fertiliser was added (49.8 kg K/ha), the application of N fertiliser at the rate of 115 and 184 kg N/ha resulted in a higher ARK value than that at the rate of 46 kg N/ha. This suggests that the simultaneous application of K and N fertiliser was able to increase exchangeable K in the soil. The application of no-tillage increased of the dry grain yield of rice (about 10%) compared with the application of conventional tillage. Meanwhile, there were significant relationships between the rice yield with the ARK and ΔK0. Moreover, the ARK was significantly correlated with K-uptake.

Silver fir (Abies alba Mill.) is still counted among drought-tolerant tree species. However, its ability to cope with the recent extremely dry period has not yet been sufficiently studied. The objective of research was to analyse differences in the climate-growth response between silver fir, Norway spruce (Picea abies L. Karst.) and European larch (Larix decidua Mill.) growing in areas with large-scale disintegration of spruce stands. In 2019–2021, the increment cores were sampled at 16 sites along the altitudinal gradient of 340–775 m a.s.l. in different regions of the Czech Republic affected by bark beetle outbreak. The radial growth pattern of fir was compared with that of spruce or larch growing under the same site conditions. In fir, the missing rings were frequently recorded during the period of peak SO₂ pollution load in 1966–1985, but they were rarely identified in recent years. In spruce and larch, missing rings were less common and occurred mainly in the recent dry period after 2015. Fir was less sensitive to summer drought compared to larch and especially to spruce, which showed high sensitivity to summer drought regardless of the altitude. The significant positive response of fir to summer precipitation was recorded at sites up to 450 m a.s.l., however, its sensitivity to drought has increased in the last two decades. Hence, when considering the wider use of fir, it is necessary to respect its ecological requirements as much as possible in order to preserve its vitality and production potential in changing climatic conditions.

Black-pigmented bacteria are one of the neglected species to cause periodontal disease in cats, and they are also zoonotic agents that pose an infection risk to humans. In this study, we aimed to determine the presence of Porphyromonas gingivalis, Porphyromonas gulae and Prevotella nigrescens in the oral microbiota of pet and stray cats. Dental swab samples were taken from 25 pet cats and 25 stray cats with symptoms of periodontal disease and then investigated by multiplex polymerase chain reaction using 16S rRNA species-specific primers. As a result of the multiplex PCR analysis, P. gingivalis 3/25 (12%), P. nigrescens 1/25 (4%), P. gingivalis + P. gulae 7/25 (28%), P. gingivalis + P. nigrescens 1/25 (4%), P. gulae + P. nigrescens 1/25 (4%), and P. gingivalis + P. gulae + P. nigrescens 2/25 (8%) were molecularly typed in the pet cats. In addition, 1/25 (4%) of P. gulae and 21/25 (84%) of P. gingivalis + P. gulae were typed in the stray cats. In 10/25 (40%) pet and 3/25 (12%) stray cat samples, no bacteria were detected by molecular typing. In summary, the results provide strong evidence that black-pigmented zoonotic pathogens are associated with cat periodontal disease.

Advocating proper phosphorus (P) fertilisation is necessary to save this limited natural resource and to save the investment in rice cultivation. This study aimed to evaluate changes in phosphorus availability, total phosphorus in soil, phosphorus buffering capacity, and phosphorus saturation in the long-term phosphorus fertilisation in the paddy rice system. Soil samples were collected in the harvest stage after seven consecutive crops over three years at Can Tho city, Vietnam. The applied phosphorus fertiliser rates were: no phosphorus fertilisation (P0), 17.4 kg P/ha (P17.4), and 26.2 kg P/ha as farmer’s practice (P26.2). The results showed that the soil phosphorus buffering capacity in P0, P17.4 and P26.2 treatments was 9.49, 9.08 and 9.04 mg/kg, respectively. The degree of phosphorus saturation of P17.4 and P26.2 treatments ranged from 17.7% to 25.5%, showing the medium to high risk of phosphorus leaching. This study indicated that the application of phosphorus rate higher than 17.4 kg P/ha might result in the reduced soil phosphorus buffering capacity in the intensive rice cropping system in the Vietnamese Mekong Delta region. Our results implied that the application of a rate lower than 17.4 kg P/ha/crop could be extended to the other rice-growing (double/triple rice) areas in the Vietnamese Mekong Delta region or other paddy rice on alluvial soils in Asia.

The degree of the phenotypic similarity and differences between 61 seedless vine varieties was studied by grouping them according to economically important phenological, agrobiological and technological indicators. A multidimensional statistical approach is applied – a cluster analysis, which provides possibilities of establishing relatively homogeneous groups (clusters), based on a set of certain indicators. As the cluster analysis does not perform any tests for the statistical significance of the results, it is combined with a factor analysis, which provides information on the reasons leading to the distribution of the individual varieties in different clusters and the merging of others into one cluster. The applied factor analysis transformed all the studied indicators into seven main components, explaining 76% of the total variance. The transformation of these traits to the corresponding number of factors and the indicators in them, which explain that the varieties belong to the separate clusters, was established. The limits of the phenotypic similarity and differences between the studied seedless vine varieties and the conditional parameters of the polymorphism with their economically significant ampelographic characteristics were determined. The presented grouping can be used for taxonomic purposes, as well as in the selection to create new seedless vine varieties. By applying targeted agro-technical measures, the desired change in the indicators of the first and second factors in the individual groups of traits can be actively influenced.

This study was planned to investigate an alternative treatment modality in diabetic wound healing. In this experimental study, the efficacy of both cold atmospheric plasma/nitric oxide (NO) and NPH insulin ointment, recently known to have beneficial effects on wound healing, was investigated in diabetic wound healing. Twenty-four (24) diabetic rats were divided into four groups DC, DI, DNO and DINO (diabetic control, diabetic insulin, diabetic nitric oxide, diabetic insulin + nitric oxide groups). No treatment was applied to the DC group, NPH insulin was applied to the DI group, CAP/NO was applied to the DNO group, and CAP/NO + NPH insulin was applied to the DINO group once daily for 14 days. The wound area reduction and the wound contraction rate were calculated on the basis of the tissue sections taken, and histopathological and genetic analyses were carried out. Compared to the control group, exogenous NO gas was found to be a potent antibacterial agent in the diabetic wound healing, causing a reduction in the wound area (P = 0.034), an increased contraction rate (P = 0.021), epithelialisation (P = 0.02), collagen organisation (P = 0.006) and a reduction in the number of inflammatory cells (P = 0.002). A significant increase in the expression of IL-8 mRNA was observed (P = 0.026). It was concluded that NPH insulin alone contributes to wound healing, but it is not necessary to use it together with exogenous NO gas.

Coumarins and pyridines are a promising class of naturally occurring bioactive heterocycles with unique physical and chemical properties. Compounds containing the coumarin framework possess a wide range of pharmacological, biological, and physiological activities, which makes them important for application in medicine, the food industry and agriculture. Among all coumarins' properties, it was found that coumarins may prevent fungal growth, depending on substituents linked to the coumarin core. Therefore, many coumarin derivatives have been investigated as potentially powerful agents in preventing and controlling fungal pathogens. This review summarises the latest research on coumarins and their antifungal activity to provide useful information for further developing more efficient coumarin-based fungicides.

Plant-based indicators of water status, such as midday stem water potential (Ψ_stem) and leaf stomatal conductance (g_s), are used to optimise irrigation scheduling in horticultural crops because they integrate the effect of soil and climatic conditions and the internal physiological constraints. The use of these indicators relies on experimentally acquired thresholds that relate the value of the indicator to negative effects on yield. In five irrigation treatments, we monitored yield, fruit size and the courses of Ψ_stem and g_s throughout four consecutive growing seasons. We found that Ψ_stem was more sensitive to irrigation treatment than g_s. Both indicators increased with available soil water content (ASWC) and decreased with evaporative demands of the atmosphere (ET_C). On a seasonal basis, crop load had a stronger impact on g_s than Ψ_stem. In summary, our study explored the effect of environmental conditions and crop load on plant-based indicators of tree water status and can be useful for establishing thresholds for irrigation scheduling in apple tree orchards.