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Under natural and well-managed conditions, the buffalo has good reproductive and productive indices. However, in vitro embryo production (IVEP) has been used commercially to maximise the number of elite animals. In this species, several factors (donor management, in vitro culture medium, semen, in vitro conditions, embryo transfer) still affect the IVEP results. In addition, the cost of this technique is very high for this purpose. Therefore, more studies, as well as adequate plans, are needed to achieve this objective efficiently. In this review, we discussed the current commercial status, influencing factors (in vivo and in vitro), and the progress and future challenges of IVEP in buffalo. A total of 81 references were used from 1979 to 2022. The relevant data or literature were searched using the following databases: Google, ResearchGate, Science Alert, Science Direct and PubMed, using the following keywords: buffalo oocytes/COCs, buffalo embryos, pregnancy and calving or live birth rate after embryo transfer. The best maturation, cleavage and blastocyst rates in the in vitro production of buffalo embryos were 95.8, 75.2 and 33.4%, respectively. The pregnancy and live birth rates ranged from 22.2% to 43.5% and from 15.3% to 36.5%, respectively, after the transfer of fresh embryos produced in vitro to the recipients. This review will help to contextualise IVEP in buffaloes, as well as create an adequate plan for implementing IVEP in buffaloes.

Panax ginseng Meyer is one of Asia’s most popular medicinal plants, with triterpene saponins as principal bioactive compounds. The present study investigates the possibility of ginseng cultivation in Lahaul & Spiti, Himachal Pradesh, India in the Western Himalayas focusing on growth characteristics, and ginsenoside content in the roots. Plant growth parameters increased with an increase in the crop age and reached maximum maturity at the age of five years along with the production of a good amount of seeds and roots. Root fresh and dry weight of the five-year-old plant was 142.6 g and 45.5 g, respectively, which almost doubled as compared with the four-year-old plant. The HPLC analysis of P. ginseng roots leads to the identification of 14 compounds representing 31.81 ± 2.89 mg/g of total ginsenoside contents, where Rb1, Rg2 and Re were found to be major ginsenosides with 7.53 ± 0.37, 7.04 ± 0.61 and 3.77 ± 0.26 mg/g content. Protopanaxadiol (PPD) and protopanaxatriol (PPT) represent the major classes of ginsenosides present in the ginseng roots with a 0.98 ratio of PPD/PPT. Our studies revealed that the soil and climate of the Lahaul and Spiti district of Himachal Pradesh State in the Western Himalayas are suitable for the cultivation of P. ginseng with good content of ginsenosides in five-year-old roots.

Due to a scarcity of freshwater resources, agriculture is dependent on the use of poor quality water for irrigation in arid and semi-arid regions. Hence, the effective use of poor quality water requires pioneering water management and nitrogen fertilizer practices for increasing yield and resource efficiency. This study aimed to investigate the effect of saline water levels, nitrogen fertilizer, and irrigation methods on eggplant yield, water productivity, NPK uptake, and nitrogen use efficiency. The experiment was conducted in 2019 and 2020 under drip (IM1) and surface flood irrigation (IM2). The treatments included three saline water levels i.e. canal water (SW1), ECiw=2.5 dS/m (SW2), and ECiw=5.0 dS/m (SW3) along with the three nitrogen levels of 75% (N1), 100% (N2), and 125% (N3) of the recommended dose of nitrogen. Application of saline water using IM1 reduced the ECe by 41.8% (SW2) and 34% (SW3) over IM2. The fruit yield, water productivity (WP), NPK uptake, and nitrogen use efficiency (NUE) was increased by 22%, 127.6%, 39.8%, 16.6%, 11.8%, and 23.8% under IM1 over IM2, respectively. A high saline water level under IM2 can cause more reduction in fruit yield, NPK uptake, and water use. Applying saline water through IM1 improves fruit yield, WP, and NUE by 13-32.8%, 104.1-147.3%, and 10.5-35.2% as compared to IM2. We found that saline water and N applied by drip improved eggplant yield, water productivity, and NPK uptake. It is concluded that irrigation water and nitrogen fertilizer consumption are optimized when saline water is applied through drip irrigation.

The escalation of the conflict between Russia and Ukraine had a detrimental effect on the global agricultural and food market and the price movements of essential commodities. In this study, we aim to investigate the effects of geopolitical risk on the prices of selected agricultural and food commodities using the linear and nonlinear ARDL (autoregressive distributed lag) model. Our results show evidence of the asymmetric impact of geopolitical risk on the prices of rapeseed, sugar, sunflower oil, and wheat. The findings also show no long-term link between geopolitical risk and corn, cotton, lumber, milk, oats, rough rice, and soybean prices.

While even-aged forestry is the dominating forest management system in Sweden, there is an increasing interest in Continuous Cover Forestry. Consequently, the conversion of even-aged stands into uneven-aged ones using e.g. selection cutting can be expected to become more common in Sweden. However, there are no up-to-date studies available on harvester productivity during selection cutting under Nordic conditions. Studying harvest intensity during selection cutting is of interest because lighter harvest intensities lead to higher volume growth and better-preserved forest ambience than heavier intensities. The objective of this study was to examine the effect of harvest intensity on harvester productivity during selection cutting. The field study entailed harvesting either 14%, 28% or 48% of a mature stand’s basal area. Harvester productivity was mainly explained by piece size (stem volume), while other factors, including harvest intensity, had only minor effects. This reality means that during selection cutting (thinning from above), piece size increases with decreasing harvest intensity, which in turn increases harvester productivity. Moreover, we observed a mild tendency that operators could select the stems’ felling directions and order more freely when fewer trees are harvested. This amelioration increases productivity additionally during lighter harvesting intensities.

The aim of this study was to determine the heavy metals concentrations in soil, inflorescences of Brassica napus and rapeseed honey sampled from some regions of north-eastern Bulgaria. Thirteen locations were selected for experimental studies. The soils, plants and honeys samples were taken from conventional beekeeping areas away from major industrial pollutants. The median amounts of elements in the soil samples (mg/kg DW) are 1.1246 for Fe(s), 0.7048 for Al(s), 0.5636 for Pb(s), 0.1658 for Cu(s) and 0.0148 for Zn(s). The median amounts of heavy metals measured in the inflorescences of Brassica napus (mg/ kg DW) are 5.5430 for Fe(p), 2.9095 for Zn(p), 1.3225 for Pb(p), 0.2593 for Cu(p) and 0.2105 for Al(p). The median heavy metals concentrations in  tested honey (mg/kg DW) are 1.0026 for Fe(h), 0.1849 for Al(h), 0.1832 for Pb(h), 0.1250 for Zn(h), 0.0702 for. Cu(h). The relationship between the heavy metal in soil, plants and honey was investigated using the Spearman’s rank correlation coefficient. Significant differences in the concentrations of Fe(s), Al(s) and Pb(s) in soils, Fe(p) and Zn(p) in the plant samples, Fe(h) in honey samples from the different locations were found. The heavy metal content tested in honey did not pose a risk to human health.

The improvement of rice production to meet food needs for the increasing population is a general problem faced in wetland development for agriculture. The use of industrial waste, such as coal fly ash (CFA), could effectively improve the soil properties of wetlands. In this study, CFA with an amount of 2% (weight/volume) or 240 g was added to 12 L of three different soils collected from the rice fields (peatland, swampland, and rainfed field) in a 15-L pot, and then incubated in the greenhouse for 15 days. The soil pH, concentrations of NH₄⁺-N, NO₃^–-N, exchangeable calcium (Ca) and magnesium (Mg) and available phosphorus in the soil were quantified following the completion of the incubation. Rice seedlings were planted in each pot, and after 90 days, the growth and yield variables were observed. The results showed that CFA application enhanced the concentrations of NH₄⁺-N, NO₃^–-N, and available phosphorus in peatland and swampland, the rice fields that contain high organic carbon (C), which ultimately leads to increasing rice growth and yield. The application of CFA to rice fields containing low organic carbon did not improve available nitrogen and phosphorus nor enhance the growth and yield of rice. Results of this study indicate an important role of soil organic C content in the rice fields in controlling the effect of CFA on nutrient availability, growth and yield of rice.

Genetic modifications can improve the health status and disease resistance of farm animals. In the previous study, genetically modified (GM) sheep overexpressing toll like receptor 4 (TLR4) were produced. The GM sheep had stronger ability to eliminate invasive microbes compared to the wild-type (WT) sheep. Physiological status and immune homeostasis, blood parameters, body composition, and gastrointestinal microbial diversity were evaluated in this study to elucidate the effects of TLR4 overexpression. In a set of 10 GM rams, the TLR4 mRNA and protein expression levels in the peripheral blood mononuclear cells, muscle, kidney, and spleen were higher than those of the WT (n = 10; P < 0.05). No significant differences in the parameters of red blood cells, white blood cells, platelets and in the composition of circulating T lymphocyte subsets were observed between the GM and WT sheep (P > 0.05). Of the biochemical parameters, only total protein levels differed (were higher) in the GM sheep than in the WT sheep (P < 0.05) while no significant differences were observed for the other indices (P > 0.05). GM and WT sheep showed similar offal weights and muscle fatty acid and amino acid compositions (P > 0.05). Furthermore, TLR4 overexpression did not affect the community structure of the gastrointestinal tract microbiota. Eleven KEGG pathways associated with the TLR4 gene, physiology and biochemistry, growth and metabolism, fatty acids, and amino acids did not demonstrate significant differences between the GM and WT sheep (P > 0.05). In conclusion, TLR4 overexpression had no principal or adverse effects on the physiological development and health of sheep, except for the general mRNA and protein expression levels.

Water and nutrient use efficiencies are important adaptive features of plants in arid and semi-arid environments. In this study, water, nitrogen (N), and phosphorus (P) use efficiencies of Stipa purpurea Griesb., an endemic and dominant grass species, were investigated in the alpine steppe along precipitation gradients on the Tibetan Plateau. The leaf N content of S. purpurea increased along the precipitation gradient, but leaf P decreased, whereas carbon (C) remained unchanged. Leaf δ¹³C (water use efficiency, WUE) and C : N ratio (N use efficiency, NUE) decreased with increasing precipitation. But leaf C : P (P use efficiency, PUE) and N : P ratios increased with increasing precipitation. A trade-off was found between WUE and PUE but not between WUE and NUE. The changes in leaf traits were associated with changes in soil water, organic C, total N and P. These findings offer insights into understanding alpine plant water and nutrient use strategies along a precipitation gradient, as well as facilitate the prediction of alpine ecosystem responses to precipitation changes.

This study aims to investigate using different ratios of urea-molasses in silage of food in vitro fermentation, feed value and quality production in feed. After being withered a little, the fodder plant was fragmented into 2–3 cm long pieces. 0, 2 and 4% molasses and 0, 0.5 and 1% urea were added. The treatment groups were UM1 (control), UM2, UM3, UM4, UM5, UM6, UM7, UM8 and UM9 supplemented with 0, 0, 0, 1, 1, 1, 1.5, 1.5, 1.5% urea and 0, 2, 4, 0, 2, 4, 0, 2, 4% molasses respectively, and a total of 54 silage samples, 9 × 6 parallel, were prepared, vacuumed in ready-made silage bags, and stored at 25 ± 2 °C for 60 days. After the silages were opened, pH, nutrient contents, Fleig scores (FS), in vitro digestibility parameters, energy contents, feed value and feed quality were determined, then the data were subjected to analysis of variance. The effects of urea and molasses additives added to the mixed forage plant silage on all parameters were found to be significant (P < 0.05, P < 0.01). The crude protein (CP) increased its application by UM7. Cell wall component (insoluble fibre in acid detergent (ADF) and neutral detergent insoluble fibre NDF) reduced its application by UM9. All these applications increased the FS. The addition of molasses increased the in vitro gas production, while the addition of urea decreased the production. True organic matter digestibility increased with the addition of molasses. As a result, it was concluded that UM9 application in silages would be appropriate for increasing feed value and feed quality, and the UM3 application in silage would be appropriate for improving true organic matter digestibility (TOMD).

Earthworms dominate the soil biota, and different structural and functional features of their biology and ecology have been studied and exploited to evaluate their contributions as ecosystem services. Due to their feeding ecology, burrowing and casting activity, earthworms are involved in the nutrient cycles, and therefore it is opportune to be considered when the biogeochemical cycles of the terrestrial ecosystems are analysed. All structural, microbiological and biogeochemical impacts of earthworms in soil start with their feeding and digestive functions, which end in casting. The casting activity consisting of the excretion of the ingested soil and organic matter after digestion processes depends on earthworm feeding behaviours and ecology, even described in the current literature as a new ecological feature: the casting ecology. The complexity of the chemical relationships occurring inside earthworm casts between main nutrients (organic carbon, nitrogen, phosphorus, potassium, calcium) highlights the complexity of the biogeochemical cycles and the great earthworms’ contribution to these cycles in the ecosystems towards a better understanding of the soil sustainability through the soil biodiversity contribution. Due to this great contribution, the earthworms’ casts should be included as indicators in the integrative conservation management of the ecosystems, as a re-thinking of the concept of ecosystem sustainability.

By choosing appropriate candidate reference genes (CRGs) and standardizing qPCR data, more accurate experimental data can be obtained. Herein, the expression stability of alpha-tubulin1 (TUA1), beta-tubulin (TUB), beta-tubulin 1 (TUB1), beta-tubulin 5 (TUB5), actin 1 (ACT1), actin 97 (ACT97), molecular chaperone dnaj (DNAJ), adenine phosphoribosyl transferase (APT), and histone H4 (HIS4) genes from Paulownia fortunei (Seem.) Hemsl. under different experimental conditions (different tissues, drought, salinity, Cd, and Cr treatments) was assessed with four statistical tools: RefFinder, BestKeeper, NormFinder, and geNorm. Notably, TUA1 and TUB5 were identified as CRGs for different tissues, ACT97 and TUB1 for drought treatment, ACT97 and APT for salinity treatment, TUB1 and ACT97 for Cd treatment, and DNAJ, TUB1 and TUB5 for Cr treatment. Furthermore, the results of “total” group, V₄/V₅ > 0.15 and V₅/V₆ < 0.15 revealed that the CRGs or gene combinations, which could meet all the test conditions, were not easy to identify. To further verify the reliability of CRGs, the expression levels of paulownia fortunei cellulose synthase A catalytic subunit2 (PfCesA2) and paulownia fortunei glutathione reductase (GR) genes were analysed. The expression patterns were different when the unstable CRGs were used for normalization compared to when the stable CRGs and combination were used for normalization. This study will lay a foundation for study on the expression levels of key genes from P. fortunei seedlings.

Dried fruits are popular ready-to-eat snacks. However, due to cross-contamination or contact with infected persons, Escherichia coli could be present, of which some strains are pathogenic, thus, the consumption of contaminated dried fruits could pose a public health risk. Microbial cells subjected to stress factors such as osmotic shock, radiation and antimicrobials could induce complex stress response systems and adaptation. Radiation and antimicrobials have been shown to cause an SOS response in E. coli, which affects cellular metabolism, such as the enhanced ability to repair DNA and mutagenesis. Studies have shown that glucose plays a critical role in the down- or upregulation of locus for enterocyte effacement (LEE)-encoded virulence of E. coli. Gamma radiation could also cause the formation of viable but nonculturable (VBNC) cells. If these cells are present in a sample, the number of viable cells will be underestimated by the plate count method leading to the false conclusion that the product is germ-free. We have reviewed in this article how stressors such as high sugar content, gamma radiation, and antimicrobials affect the survival of E. coli in dried fruits.

An intracranial arachnoid cyst (IAC) is a rare developmental disorder that is consistent with cerebrospinal fluid accumulation between the brain and the arachnoid membrane. A quadrigeminal cyst is a specific type of cyst that is identified based on its localization. To the best of our knowledge, this is the first study to report on this type of pathology in a Brussels Griffon. This case study describes an 11-month-old female Brussels Griffon with symptoms of reluctance to lower the head and eat from a bowl placed on the ground, combined with episodes of vocalization. The patient was subsequently diagnosed with a quadrigeminal cyst during a low-field magnetic resonance imaging (MRI) exam, and she tested positive for toxoplasmosis in the blood test. Arachnoid cysts are often described as incidental findings, but the characteristics of neurological symptoms in the presented patient suggest that the cyst was clinically significant. The currently known options of pharmacological and surgical treatment give some hope for symptomatic patients, although their definitive success rate is not yet fully known.

Boric acid (BA) is commercially acceptable and economically feasible material to enhance the shelf life of pears, oranges and other horticultural plants. Here, we investigated the effect of BA on the shelf life and post-harvest quality of table grapes (cv. ‘Kyoho’). The grapes were immersed in a BA solution with different concentrations [0 (as the control), 10, 30, 50 mM] for 10 min and stored at 25 ± 1 °C for 10 days. Compared to the control, the BA treatments maintained higher berry firmness by inhibiting the activity of polygalacturonase (PG) and cellulase. In addition, the BA-treated grapes maintained higher antioxidant enzyme activities, such as catalase (CAT) and superoxide dismutase (SOD), and lower metabolic toxic products, like the superoxide anion (O2-) production rate, malondialdehyde (MDA) and hydrogen peroxide (H₂O₂) content than the control. The experimental results showed that the post-harvest application of BA effectively delays the senescence of grapes compared with the control, and the 10 mM BA treatment had the most obvious effect.

Tree height is one of the most important variables in describing forest stand structure. However, due to difficulty in height measurement, especially in dense and mountainous forests, the common approach is to invoke the height-diameter (H-D) models. The oriental beech (Fagus orientalis Lipsky) is one of the most important species of Hyrcanian forests, over the mid to high-altitudes (400–1 800 m a.s.l.), in northern Iran. In this study, the H-D relationship of beech trees was investigated separately for mid-altitude and high-altitude in Shafaroud forests of Guilan using 14 nonlinear H-D models and an artificial neural network model (ANN). To collect data, a systematic random sampling method within a 100 × 100 m regular randomized grid was applied. In total, 3 243 individual trees in 255 circular plots with 0.1 ha were measured. For comparing the results, performance criteria including root mean square error (RMSE), R²_adj, Akaike's information criterion (AIC), and mean absolute error (MAE) were used. In high and mid altitudes, Meyer (1940) and Bates and Watts (1980) models had the best performance, while Watts (1983) model and Burkhart-Strub (1974) model had the worst performance in high-altitude and in mid-altitude, respectively. On the other hand, the ANN model had the best accuracy and performance in both sites. Since the performance of the ANN model is superior and consistent compared to the common nonlinear models, here it is preferred for both regions.

Glyoxal oxidase (GLOX, EC 1.2.3.15) is a class of hydrogen peroxide synthases involved in lignin degradation. Several GLOX genes of biotrophic and necrotrophic phytopathogenic fungi have been confirmed to contribute to fungal growth, development, and pathogenicity. However, the composition, sequence characteristics and functions of genes in the GLOX family of hemi-biotrophic phytopathogenic fungi remain poorly understood. This study identified a GLOX-encoding gene CsGLOX in the hemi-biotrophic phytopathogenic fungus Colletotrichum siamense by bioinformatics analysis and PCR cloning. The encoded protein contains a signal peptide and five cell wall stress-responsive component (WSC) domains at the N terminus, followed by a catalytic structural domain (AA5_1). CsGLOX-deficient mutants were constructed by homologous replacement, which significantly increased the formation of conidia and reduced the pathogenicity and the high-osmotic stress tolerance of the fungus. However, the deletions did not influence fungal filamentous growth. This study proposes that the CsGLOX gene is involved in pathogen conidial formation, pathogenicity, and osmotic stress response, which deepens our understanding of the pathogenic mechanism of hemi-biotrophic phytopathogenic fungus C. siamense.

We conducted a quantitative ex post analysis of the competitiveness of the Czech Republic’s agrifood exports to other European Union (EU) Member States between 2002 and 2020, using the constant market share (CMS) methodology. The application of this methodology to Czech agrifood exports is novel, as it allows for analysing the export competitiveness variation and its components. Although the findings are consistent with those of previous studies in which the investigators used alternative methodological approaches, we make three contributions to the existing literature. Firstly, the Czech Republic experienced an overall increase in its competitiveness in agrifood exports between 2002 and 2020. Secondly, the high territorial specialisation of Czech agrifood exports to other EU Member States, combined with a general slowdown in the growth of EU markets, has had a significant effect on the growth dynamics of the country’s agricultural exports. Thirdly, there is a problem with the specialisation profile of Czech agrifood exports in terms of commodity and territorial structure. The current composition does not allow the Czech Republic to reach its full potential for agrifood exports. These findings will help industrial, agricultural and trade policymakers to assess the nature of liberalisation and transformation of the Czech Republic’s agricultural sectors, with a view to improving or designing structures and instruments for the promotion of agrifood exports.

Neoplasms account for approximately half of the deaths of dogs over ten years of age. This finding, associated with the fact that canine cancer patients will often suffer from the consequences of the systemic spread of the tumour and paraneoplastic syndromes, shows the importance of understanding palliative care by veterinarians and owners. In view of this, this review aims to address palliative care that can be used in veterinary medicine to promote the patient’s well-being and quality of life.

Teak has been planted extensively by smallholders in Central America within reforestation programmes and has become one of the most valuable timbers. The five-year growth and mortality of teak cultivated in a plantation in central Nicaragua, representing sites at the low limit of teak ecological valence, were evaluated. From 2006 to 2010, 72 pure teak stands were established, with 48.93 ha in total. For afforestation, planting stock from five provenances was used and planted at 1 m × 1 m spacing to stimulate the height growth and reduce broad crowns formed by self-pruning. In the pure teak stands, 144 permanent sample plots of 0.01 ha in size were established in 2011. From 2011 until 2015, the tree height and stem girth of all individuals in the studied sample plots were measured, and mortality based on the stand density was assessed. Significant differences between the provenances were observed. The highest growth was noted in the Local provenance originated from the studied area, whereas the Semilla provenance from Costa Rica, characterised by the lowest growth ability, was characterized by lower radial increment and mean tree height with high mortality.

This paper presents the experimental research of factors influencing the UDS 214 excavator efficiency. The hydraulic oil flow rate is measured in hydraulic circuits for controlling the moving of the bucket of the UDS 214 ex­cavator. From the measured values, the total power losses of the individual hydraulic circuits and their efficiencies are evaluated by calculations and measurements. Furthermore, the times of the excavator cycles during a soil excavation and loading of the transport vehicle were measured. From the measured operating cycle times of the excavator, the average value of the operating cycle time was evaluated and, from this average time, the theoretical performance and the operating performance of the given excavator in the given operational states were calculated. Then, at the end of the paper, the individual calculated power losses as well as the efficiencies of the hydraulic circuits for controlling the moves of the excavator are evaluated. According to the findings, the swing hydraulic circuit of the excavator, which has the second highest power loss of 5.926 kW and its percentage in the average tested cycle time of the excavator is 48%, seems to be a suitable hydraulic circuit for the innovation.

Nutrition is an important part of the critical care. The goals of this prospective clinical study were to create a scoring system for the assessment of patient nutritional status and to compare continuous and intermittent bolus feeding of enteral nutrition (EN). A total of 38 patients (21 dogs and 17 cats) were divided into Group C (continuous feeding; 23 patients) and Group I (intermittent feeding; 15 patients). The following variables were recorded for each patient in both groups: gastrointestinal (GI) complications, changes in body weight (BW), body condition score (BCS), muscle condition score (MCS), veterinary nutritional status score (VetNSS), length of hospitalisation and outcome. The normality of the data was assessed using the Shapiro-Wilk test. Fisher’s exact test, Mann-Whitney, Wilcoxon signed-rank tests, and the t-test were used in statistical analyses. Significant changes were found in VetNSS and BW between the 1^st and 5^th day in Group C. The VetNSS seems to be useful for monitoring the nutritional status of hospitalised patients. Anorectic dogs and cats can be successfully supported with either continuous or intermittent feeding methods with a similar risk of GI complications and outcomes.

The roasting coffee process is the compilation of time-temperature-dependent physical and chemical transformations induced by heat. Through the study of the time-temperature curve, the correct definition of the degree of roasting of coffee is an area of active and non-trivial research, which intervenes in obtaining consistent roasting profiles reflected in the acceptability of the drink perceived by consumers. This paper used the methodology of response surfaces (RSM) with the central composite design (CCD). A consumer study was conducted with 104 individuals, each tasting 13 cups of specialty coffee from Cauca-Colombia. Predictive equations for acidity and body perception were obtained. The roasting profile points in charge, turning point, yellow, brown, first crack, and drop step were included. Mathematical models predict the acidity and body perceptions by integrating the roasting profile points characteristics of coffee. The Cauca coffee beans produced using the optimied conditions contribute to obtaining a roasting time/temperature of 468 s at 192 °C, respectively, with acidity (9/15) and body (5.5/15) in the (0–15) intensity consumer's perception scale.

The 16-year-old female leopard gecko (Eublepharis macularius) was presented with distended coelom and cachexia. Examination of the faecal sample ruled out the presence of protozoan parasites. A radiographic examination confirmed the presence of radiopaque foreign material in the intestine. The conservative treatment with tramadol, butylscopolamine, famotidine, vitamin B complex, and supportive fluid therapy with Hartmann solution and Duphalyte, was performed for 14 days. Ultrasonographic examination revealed the presence of a large mass adherent to the liver (with hypoechoic regions), a thin-walled cystic structure close to the liver, and coelomic effusion. Surgical exploration revealed a large mass on the right ovary. The unilateral (right) ovariectomy was performed. Histologic examination of the mass revealed dysgerminoma with an invasion of the ovarian bursa and blood vessels. Nine months after the surgery the patient was active and doing well. In reptiles, dysgerminoma is an uncommon type of neoplasia. To the best of our knowledge, this is the first case of dysgerminoma tumour diagnosed intravitally and treated successfully in lizards.

A greenhouse study was conducted to test the effects of low herbicide dose exposure on different crops measuring visible damages, plant height, leaf area, and dry matter. Seven crops were tested: lettuce (Lactuca sativa L.) cv. Novosadska majska maslena, oil pumpkin (Cucurbita maxima Duch) cv. Olivija, oilseed rape (Brassica napus L.) cv. NS Ras, pepper (Capsicum annuum L.) cv. Kurtovska kapija, soybean (Glycine max (L.) Merr) cv. ZP Laura, sunflower (Helianthus annuus L.) cv. NS Kruna, and tomato (Solanum lycopersicum L.) cv. Dunavski Rubin. Herbicide dicamba in the range of 0.14 to 1 155.6 g a.i. (active ingredient)/ha inhibited biomass, height, leaf area, and visual injury of all crops, while glyphosate doses from 0.48 to 3 840 g a.i./ha also reduced the growth of all tested species. A rate of 116 g a.i./ha mesotrione was needed to reach 80% visual injury in oilseed rape, while the same effects on lettuce only required 1.8 g a.i./ha of mesotrione. Tomato and oil pumpkin were also sensitive to low mesotrione doses, where only 1.3 g and 0.5 g a.i./ha of mesotrione was needed for 80% of biomass reduction, respectively. Lettuce was the most sensitive crop of all tested species; biomass was reduced by 80% by dicamba, glyphosate, mesotrione, and nicosulfuron at the low rates of 33 g a.i./ha, 19 g a.i./ha, 1.25 g a.i./ha, and 2.7 g a.i./ha, respectively. Among all herbicides, visible injuries were detected in dicamba at the lowest rates. Soybean was the most tolerant of glyphosate, mesotrione, and nicosulfuron. Based on the available literature and obtained results, herbicide off-target movement must be mitigated to maximise herbicide efficacy and decrease the negative influence on susceptible plants and the environment.

Glyphosate is a widely used agrochemical. Nevertheless, only a few studies have investigated its effect on bees, specifically its influence on their foraging activity. This article provides a summary of the prominent research results on this issue, published in journals in the field of experimental biology. The effect of commonly used concentrations of glyphosate on honey bee navigation has been evaluated in several studies, as well as concentrations that are reportedly sublethal. Exposure to this herbicide increases the flight time back to the hive and affects the flight trajectories of these bees. These results imply that glyphosate at certain concentrations reduces their sensitivity to nectar rewards in associative memories. The contact of bees with non-lethal concentrations of glyphosate results in sublethal effects that affect foraging. In the future, the behaviour of glyphosate and its effect on bees in their natural environment need to be explored.

The presented study is concerned with infectivity of select entomopathogenic nematode species under different temperature conditions (15, 20 and 25 °C) in the presence of competing species. Two species of nematodes of the genus Steinernema (S. kraussei, S. feltiae) and two of the genus Heterorhabditis (H. bacteriophora and H. megidis) were included in the analysis. Different experimental variants were adopted in which the selected two entomopathogenic nematode species were mixed between Steinernema and Heterorhabditis. The study showed that Heterorhabditis and Steinernema cannot coexist together in a single host and one genus will always prevail. H. megidis, under co-occurrence with S. feltiae separately infected Galleria mellonella larvae most commonly at 15 °C, while H. bacteriophora at 20 °C. The study showed that the main determinant of nematode activity towards the host is not temperature, but the presence of co-existing nematode species. The results of the experiment encourage further research to determine the effects of a variety of concurrent biotic and abiotic factors on entomopathogenic nematodes and their biological activity.

Modern single space feeding systems for fattening pigs allow the detailed assessment of an individual animal's feeding behaviour. In an experiment involving 96 fattening pigs, the influence of deoxynivalenol (DON) contaminated feed (> 4 500 µg/kg DON) on the zootechnical performance and feeding behaviour was compared with a feed with low DON concentration (< 900 µg/kg DON), this served as the control group. Additionally, in separate treatments, two commercial mycotoxin binders were added to the DON-contaminated feeds to assess if an expected DON effect could be attenuated. The high DON content significantly (P < 0.03) reduced daily feed intake (500-600 g/day). The DON group showed 240 g less daily gain compared to the control with 728 g/day. Both mycotoxin binders were seen to additionally depress weight gain by approximately 65 g/day (P < 0.01). The treatment did not affect the individual feeding behaviour as assessed by daily visits to the feeder, feed intake per visit and the highest feed intake per visit per day. These were influenced only by the pig and its pen, indicating that the animals developed a distinct behaviour within their respective groups. Behaviour analyses of persistency and day-to-day variation showed that the persistency was reduced and variation was increased when DON without or with binder was present. The DON contents therefore seemed to lead to a more erratic and less consistent behaviour that remained dependent on the animal group.

In the last few years, it has become easier to diagnose congenital brain malformations in dog's ante-mortem. This is probably due to the wider availability of advanced diagnostic imaging, in particular computed tomography or magnetic resonance imaging (MRI). Despite this, there is still not much literature available about potential treatments, outcome and overall prognosis with respect to the patient's quality of life. We present three cases of Dandy-Walker malformation in Polish hunting dogs with up to five years follow up including assessment of their quality of life. A nine-week-old Polish hunting dog was presented with progressive signs of ataxia that had developed over the preceding few weeks. MRI revealed a Dandy-Walker malformation. The dog underwent MRI at the ages of 12 and 36 months, and his neurological condition was followed for five years. No other subsequent abnormities were found on further diagnostic tests. The dog showed transient behavioural problems which improved after castration and training. The ataxia seen at first visit improved with time and intensive physiotherapy, and the dog was considered to have a good quality of life in the owner's eyes. A further two cases shared a similar disease course. Dandy-Walker malformation was confirmed with MRI, but diagnostic imaging could not be reported here because the owner did not consent to publication. The Dandy-Walker malformations described in these three Polish hunting dogs had a stable disease course and revealed that dogs suffering from this condition may have a good quality of life, providing no other abnormalities are detected and rehabilitation is performed.

Phytoplasma-like symptoms of leaf yellowing and calyx malformation were observed in eggplant (Solanum melongena L.), upward leaves and fruit malformation in pepper (Capsicum annuum L.), and aerial tuber formation in potato (S. tuberosum L.) during the survey performed in the late season (August to September) of 2015 and 2016 in Van province (Turkey). A total of 100 samples were tested by nested-PCR using universal primer pairs to assess the sanitary status of the solanaceous crops and to characterise the phytoplasma isolates. Among them, seven samples resulted in a 1.25 kb DNA fragment, and five (two eggplants, two peppers, and one potato) were molecularly characterised (Accession No.: KY579357, KT595210, MF564267, MF564266, and MH683601). BLAST and the virtual restriction fragment length polymorphism (RFLP) analysis of 16S rRNA genes revealed the presence of two distinct phytoplasma infections in solanaceous crops: 'Candidatus Phytoplasma trifolii' a member of the clover proliferation group (16SrVI) and subgroup A and 'Candidatus P. solani' a member of the stolbur group (16SrXII) and subgroup A. The virtual RFLP analysis and calculated coefficients of RFLP pattern similarities further revealed a remarkable genetic diversity among the 'Candidatus P. solani' isolates infecting pepper (similarity coefficient of 0.90) and eggplant (similarity coefficients of 0.98 and 1.00) at the same geographical area. This is the first report of the natural occurrence of 'Candidadtus P. trifolii' in potato from the Eastern Anatolia region, Turkey.