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The aim of this work was to study the effect of milking fraction and mammary gland health status on the electrical conductivity (EC) of milk from Manchega ewes, considering also the lactation number. To this end, we also studied the relationship of EC with milk macrocomposition, and the relation existing between EC and somatic cell count (SCC). Finally, the use of EC thresholds as a mastitis detection method (sensitivity, specificity, positive (PPV) and negative predictive value (NPV)) was assessed in each of the three fractions: first streams (F1), machine milk (F2) and stripping milk (F3). Milking fraction, mammary gland health status and lactation number had a significant effect on EC and SCC. In the case of EC, the milking fraction caused a more pronounced effect than health status of the glands or number of lactation (F = 19.95, 15.88 and 6.55, respectively; P < 0.5). In SCC, the gland health status caused the most pronounced effect followed by milking fraction and lactation number (F = 112.02, 6.89, and 5.28, respectively; P < 0.05). Changes in the milk composition, especially fat and lactose contents, explained the EC variation to a great extent. For the same EC threshold, specificity and sensitivity varied slightly depending on the milking fraction. NPV above 80% was obtained in the three milking fractions and at all EC thresholds tested, but PPV was only higher than 20% as of the threshold of 4.5, 4.4, and 4.2 mS/cm in F1, F2 and F3, respectively. From the results obtained, we concluded that the algorithm design for mastitis detection in sheep should include those factors affecting the composition and which therefore cause variations in EC, such as milking fraction, individual differences, lactation stage or lactation number.

Whole genome sequences of three Czech Pea seed-borne mosaic virus isolates belonging to P1 pathotype and causing different symptom intensity were obtained. Using RDP4 analysis the natural recombinant isolate PSB204CZ bearing two breakpoints in nucleotide positions 4053 and 6080 was identified. The isolate was composed of fragment 2028 nt in length partially covering CI and 6K2 regions of the minor parent (PSB262CZ) incorporated into the major parent (PSB194CZ). The results suggest that the observed recombination in CI-6K2 region is responsible for severity of developed symptoms. This observation detected for the first time natural recombination within PSbMV isolates of an important pathogen of leguminous plants.

Crop growth is a key factor that effects nitrous oxide (N₂O) emission in farmland soil. Clarification and quantification of the impact of maize growth on N₂O emission are important to guide maize planting and patterns, which is also useful for building model to simulate N₂O emission in an agroecosystem. In this study, we carried out a three-year (2013-2015) field experiment to evaluate the contribution of maize growth on N₂O emission using a split-plot design. The factors included planting versus not planting maize, and four rates of nitrogen (N) application (0, 150, 300, 450 kg N/ha). Our results showed the impacts of maize growth on N₂O emission decreased linearly with the growth of maize from the 43^rd day after sowing (y = -1.07x + 26.85, R² = 0.95). Nitrogen fertilizer application can reduce the impacts of maize growth on N₂O emission. The impact of maize growth on soil NH₄⁺-N and NO₃^--N are similar to N₂O emission, and they have a strong correlation. We concluded that maize growth reduces soil N₂O emission but N application can exert an antagonistic effect, and the impact of maize growth on soil NH₄⁺-N and NO₃^--N largely determines the impacts of maize growth on N₂O emission.

One of the most significant environmental problems in Europe is the land use change as a result of urbanization. The estimate of future agricultural land takes in the Czech Republic previously published in this journal is alarming; however, this is based on arbitrarily determined assumptions. Our contribution brings a more realistic assessment of the extent of expected land takes (example of the Hradec Králové Region). For this purpose, the data from the municipalities' Planning Analytical Materials (PAM) on buildable areas (and redevelopment areas) and data on the existing expansion of built-up areas are used. Particular attention is paid to the best quality soils included in the 1^st and 2^nd protection class of agricultural land resources (ALR), because some municipalities located in fertile agricultural areas argue about the necessity to build up good-quality land. The Pearson correlation coefficient has been used for the evaluation to what extent the share of the soils included in the 1^st and 2^nd protection classes of ALR out of the total area of the municipality is really related to the share of best quality soils in planned buildable areas. The spatial statistics method ‒ geographically weighted regression (GWR) has been used to find spatial deviations from the global relationship model. There is a clear differentiation between the municipalities as to whether they are able to rather protect the best soil or whether they are planning future construction predominantly on it. E.g. in municipalities with about 30-50% of the land included in the 1^st and 2^nd ALR protection classes, buildable and redevelopment areas are designed from 0 to 100% for these highest classes of ALR protection. However, the total strength of the association (Pearson's r) between these indicators is large, r = 0.80 (or r = 0.95 when "the point-index value of agricultural land" was used instead of ALR protection classes). The results of GWR show that higher deviations from the model value, both positive and negative ones, are not spatially clustered but located next to each other. Greater deviations occur more frequently in the more fertile western part of the region, where there is a higher pressure on good-quality land, which is either intended for development or protected on the basis of local factors (including spatial planning of individual municipalities). Estimation of future developments has revealed a substantial over-dimensionality of planned buildable areas - they will potentially be built up in more than 100 years.

The paper pertains to the issue of quality assurance methods. Particular emphasis is placed upon the examination of hazards and possible product defects in food processing. At present, Polish regulations oblige any food processing entity to implement HACCP system. The system is perceived as a universal vaccine preventing the emergence of possible hazards or reducing the risk to an acceptable level. As far as defects are concerned, these are either disregarded altogether or do not occur at all. Meanwhile, the practice proves the case is not so. The research aspect of the paper revolves around an attempt to transplant solutions primarily applied in the automotive sector onto food processing industry. The practical aspect of the paper is manifested in the analysis of causes behind defects and hazards conducted on the basis of bakery industry. Potential hazards and product defects emerging from the process were identified in the course of the study. In addition, in order to eliminate these, their level was established with the application of various methods.

Endophytic fungi are widespread in plants and affect the host fitness and population size. We found 12 fungal taxa in C. secalina, a critically endangered species in several European countries, at two study sites in Poland. The most frequently occurring fungal taxa were Colletotrichum destructivum and Acremonium sp. Both taxa were found in half of the examined tussocks. The highest number of fungal taxa was noted in the C. secalina plants growing in the roadside area, where 7 of the 12 identified fungal taxa occurred. These fungi, inhabiting leaf tissues, are known for their pathogenicity but no visible symptoms of any diseases were observed on C. secalina leaves. This suggests that these fungi are latent pathogens.

A field experiment was carried out in the years 2012-2018 in Poland in a split-plot design. The aim of the study was to determine the long-term effect of legumes as forecrops on the productivity of rotation with nitrogen fertilization. The rotation included: legumes + spring barley (SB), winter rape (WR), winter wheat (WW) and winter wheat. The study was conducted as a two-factorial field experiment with four replications. The present study showed that legumes as forecrops increased the yield of all after-harvest crops in rotation. Yielding of these crops also depended on nitrogen fertilization and position in the rotation. After comparison of the influence of nitrogen fertilization on yield of cereals, it was observed that the effect of this factor was greater for WW cultivated in the fourth year of rotation than for WW cultivated in the third year of rotation. In relation with control, each dose of nitrogen fertilization caused a significant increase of WR and cereals yield, but the dose of 180 kg N/ha did not increase yield significantly in comparison to the dose of 120 kg N/ha. There was also negative agronomic N-efficiency observed between doses of 120-180 kg N/ha, which means that it is not necessary to use 180 kg N/ha, especially if there are legumes in crop rotation.

The water demand for cover crops (CC) should be considered to achieve competitive crop stands for weed control also under unfavorable conditions. This study aims to estimate the weed suppressive ability of winter CC, as Sinapis alba L., Phacelia tanacetifolia Benth., Vicia sativa L. and Avena strigosa Schreb., under a water-limited regime. The water deficit tolerance of different CC was determined in a greenhouse experiment by measuring the maximum quantum efficiency of photosystem II. Moreover, soil moisture, CC, and weed establishment were measured in field experiments in Southwest-Germany during two contrasting growing seasons in 2016 and 2017. A. strigosa showed a higher water deficit tolerance than S. alba in the greenhouse. In the field, A. strigosa showed the highest weed cover reduction (98%) in the field, along with an increasing effect on the soil moisture compared to the untreated control. S. alba performed most sensitive to water deficit in the greenhouse but reached the significantly highest weed control efficacy (94%) during the dry field season in 2016. Even though the selected CC showed differing sensitivities to water deficit in the greenhouse, their weed suppression ability was independent of the water supply under field conditions.

Considering the importance of recreation and excursions in household's basket of expenditures on the one hand, and not paying attention to the economic value of recreational function for natural resources and environment as non-market goods on the other hand, this paper was written with the aim of estimating the recreational value of Shahid Zare Sari Forest Park when using the individual travel cost method. Requested data sets were obtained by completing questionnaires from 302 individuals who visited the above-mentioned park in 2012-2013. In the individual travel cost method, for the investigation of distributional effects of variables on the number of travels, a linear regression model was applied. For this purpose the travel generation function using linear, double log, linear-log and log-linear forms was estimated. Among these forms, according to the econometrical parameter, a linear function was selected and parameters were estimated based on the method of ordinary least squares. The consumer surplus was 12.53 USD per each visit and the annual recreational value of 72,500 people who visited the park annually was 52,558 USD.ha^-1. The findings also showed that the involved variables, travel costs, income and visitor's distance were statistically significant at 1% level and family and education were significant at 5% level. Results revealed that forest parks have a considerable recreational value that, from this point of view, can help programmers and executives, social and economic managers in the preservation planning and sustainable utilization of natural resources.

Land, water, sun, infrastructure, capital and know-how are needed for any agricultural development. Sub-Saharan Africa has immense natural resources, though often not immediately available altogether in the same place, but is generally short of the other inputs. That is why a public-private partnership can be an effective approach to deal the projects with modern agricultural development: public partner provides land, most of the infrastructure and finance; private partners provide the intensive farming practice, processing know-how and part of the equity. Financial analysis of lower and higher capital demanding scenarios and testing of the impact of changes in the critical drivers of costs and revenues shown that a combination of staple crops and cash crops can be found to balance national food security policy targets and financial appeal for private partners in a mutually satisfactory venture capital. The effect of environmental and infrastructural constraints was also considered, showing how likely-to-happen threats on the side of the implementation of the project may turn into challenging opportunity to climb the agribusiness value chain upward.

Changes in mango fruit quality, malondialdehyde content, and enzymatic activities in response to pathogen Alternaria alternata infection were studied. A. alternata significantly affected the appearance of mango fruit at 5 and 7 days after treatment (DAT). The quality of pathogen-infected fruit first showed a significant decrease in titratable acidity and vitamin C content and a significant increase in pH since 3 DAT. The malondialdehyde content was higher than that in the untreated controls at 3 and 7 DAT. The enzyme activities of ascorbate peroxidase and polyphenol oxidase showed significant increases since 3 DAT. Significant increases in l-phenylalanine ammonia-lyase and superoxide dismutase activities were observed at 7 DAT. These results indicate that A. alternata infection first significantly affects some biochemical constituents and enzyme activities in mango fruit since 3 DAT and that there was no significant effect on appearance until 5 DAT.

The virulence of the Isaria fumosorosea strain (Ifu13a) against different plant sap-sucking insects such as Jacobiasca formosana Paoli (Hemiptera: Cicadellidae), Aphis gossypii Glover (Hemiptera: Aphididae), Bemisia tabaci Gennadius (Hemiptera: Aleyrodidae), and Stephanitis nashi Esaki et Takeya (Hemiptera: Tingidae) in laboratory condition at 21 ± 1°C temperature and 78 ± 5% relative humidity was determined. We found that the Ifu13a strain had excellent potential to control the target insects. The mortality of the tested insect species ranged from 81 to 100% in the concentration of 1 × 10⁸ conidia/ml. However, the lowest mortality of 33% was observed in the concentration of 1 × 10⁵ conidia/ml against the S. nashi population. Median lethal times (LT₅₀) were obtained from a regression-probit value which indicated 4.1, 4.1, 4.8, and 7.3 days at a concentration of 1 × 10⁸ conidia/ml, whereas, median lethal concentration dosages (LC₅₀) were calculated as 3.9 × 10³, 6.8 × 10⁴, 3.0 × 10⁴, and 6.9 × 10⁵ conidia/ml against J. formosana, A. gossypii, B. tabaci, and S. nashi, respectively. The present study showed that the Ifu13a fungal strain is highly pathogenic to the target insects, and it can be used as a biocontrol agent against plant sap-sucking insect species under favourable weather conditions.

The aim of the study was to evaluate the glomalins content (total glomalin (TG), easily extractable glomalin (EEG) and glomalin-related soil proteins (GRSP)) in the soil under winter wheat from different crop production systems. The experiment involved four different cultivation systems: organic, integrated (INT), conventional (CON), monoculture-conventional (MON). The highest content of TG and GRSP proteins were observed in organic system. A strong positive correlation was observed between the total number of glomalins and dehydrogenase activity and organic matter. A strong correlation between TG and GRSP content was observed (r = 0.93) as well as between EEG and GRSP (r = 0.79). The highest yields of winter wheat were observed in CON (9.12 t/ha) and INT (9.04 t/ha) systems, while the lowest in monoculture (4.47 t/ha).

Cattle slurry is commonly used to fertilize grasslands, so its impact on plant composition and herbage properties is important. Cattle slurry at annual rates of 60 (S1), 120 (S2), 180 (S3), and 240 kg nitrogen (N)/ha (S4) was applied to Arrhenatherion grasslands in moderately wet (WS), slopy (SS), and moderately dry (DS) sites cut three times a year over six years, to assess its effects on plant functional types, the Ellenberg N indicator value (Ellenberg N), herbage dry matter (DM) yield, herbage N content and offtake, N nutrition index (NNI), and N use efficiency (NUE). The site-specific changes in an increase in graminoid cover, Ellenberg N, herbage DM yield and N offtake, and NNI along with slurry application rates revealed, while cover of legumes, short forbs, and NUE decreased. In more productive sites (WS and SS), slurry application in the amount of 180 kg N/ha could be suggested as a slurry dose ensuring beneficial agronomic objectives. However, nature conservation requirements via maintaining plant biodiversity were not met. On the contrary, short-term slurry application up to 120 kg N/ha ensured on permeable DS not only sufficient agronomic objectives, but also plant biodiversity conservation requirements.

The Food and Agriculture Organization of the United Nations (FAO) emphasizes the right of everyone to have access to safe, sufficient and nutritious food in its Rome Declaration. This article suggests how this noble FAO goal can be achieved. We suggest that a first step could be the introduction of a global meat tax, where the size of the negative externalities from meat production could be calculated based on foresight and big data. Applying the tool of a global meat tax will lead to a "double dividend" as negative externalities are reduced and at the same time huge tax revenues will be generated which could be used to make further steps in the direction of achieving the stated FAO goal in the Rome Declaration.

Germinated seeds are rich in various nutrients but are vulnerable to fungal contamination which favours micromycete formation on the sprouts. The main aim of this work was an investigation of strategies to reduce the contamination of sprouted seed foods. Over the course of 96 hours of sprouting, seeds of organic spring wheat (Triticum aestivum L.), winter wheat (Triticum aestivum L.), naked oat (Avena nuda L.), triticale (xTriticosecale) and rye (Secale cereale L.) were irrigated in water filtered using the Pazdroid Med-1500 filtration device with and without 4% ethyl alcohol. Germinated seeds were stored at 18°C for one, three and seven days and the levels of Mucor spp., Penicillium spp., Alternaria spp., Aspergillus spp., Fusarium spp. and Bipolaris spp were determined. Micromycete numbers were greater in sprouted winter wheat and rye but were reduced when these were soaked and irrigated with filtered water and filtered water containing 4% ethyl alcohol. Filtered water led to greater reductions in micromycete numbers in sprouted winter and spring wheat than in other seeds.

The Antisense 4CL gene was transfected into alfalfa through Agrobacterium-mediated transfer. The test results indicated that the antisense 4CL gene was successfully integrated into the genome DNA of alfalfa and was stably transmitted to the offspring. Compared to the wild-type plants, the lignin content of T₀ and T₁ generation plants was reduced by 45.77% and 31.97%, respectively; there were no significant differences in height and weight of T₀ and T₁ plants, compared to the wild-type plants. However, the transgenic plant differed from the wild-type plant by softer stems and leaves, larger leaves, fewer flowers and a fewer seeds. The T₀ line was susceptible to disease infection, but significantly improved in the second year. The results suggest that the 4CL gene from Amorpha fruticosa can be used to regulate lignin biosynthesis in transgenic forage crops.

The trap catch of Agriotes species and dynamics of the occurrence of adult click beetles using pheromone traps were determined, and the relationship between the dynamics of the caught wireworms and the placement of traps in selected agricultural crops was investigated. The study was conducted in 2011-2014 in the fields of different crops at five localities in two regions in Poland. The effectiveness of trapping the Elateridae beetles using pheromone traps varied in different localities. The results of the analysis of variance indicate statistically significant differences in the number of A. lineatus beetles only in individual years of research. On average, most individuals of this species were observed in the first year of observation (72.62), and the least - in 2012 (18.5). Statistically significant differences in the occurrence of beetles in each month were observed for all species except A. obscurus. No statistically significant differences between populations of the tested Agriotes species were observed in different localities. Their effectiveness was influenced largely by weather conditions. Pheromone traps work well and can be a part of an assessment system for the integrated pest management of Agriotes species.

Excessive nitrogen (N) and water input, which are threatening the sustainability of conventional agriculture in the North China Plain (NCP), can lead to serious leaching of nitrate-N (NO₃^--N). This study evaluates grain yield, N and water consumption, NO₃^--N accumulation and leaching in conventional and two optimized winter wheat-summer maize double-cropping systems and an organic alfalfa-winter wheat cropping system. The results showed that compared to the conventional cropping system, the optimized systems could reduce N, water consumption and NO₃^--N leaching by 33, 35 and 67-74%, respectively, while producing nearly identical grain yields. In optimized systems, soil NO₃^--N accumulation within the root zone was about 80 kg N/ha most of the time. In the organic system, N input, water consumption and NO₃^--N leaching was reduced even more (by 71, 43 and 92%, respectively, compared to the conventional system). However, grain yield also declined by 46%. In the organic system, NO₃^--N accumulation within the root zone was generally less than 30 kg N/ha. The optimized systems showed a considerable potential to reduce N and water consumption and NO₃^--N leaching while maintaining high grain yields, and thus should be considered for sustainable agricultural development in the NCP.

In four-year experiments, hop was treated with 7 biologically active substances in two terms during vegetation: Lignohumate max (a mixture of humic acids and fulvic acids), Lexin (a mixture of humic acids and fulvic acids enriched with auxins), Lexenzym (a mixture of humic acids and fulvic acids enriched with auxins, phytohormones and enzymes precursors), Ascophyllum nodosum seaweed extract, synthetic auxin, humic acids and fulvic acids alone. The chlorophyll content was monitored after the application both in the vine leaves and in the branch leaves. After harvesting of the hops from the individual treatments, the yield of dry hops was determined and the cones were analysed for the content of alpha bitter acids. The results show that the most effective hop treatment was the application of Lexin and Lexenzym. The Lexenzym treatment provided a yield of dry hops of 1.86 t/ha, i.e. 0.47 t/ha higher compared with untreated control. The Lexin treatment provided yield higher by 0.41 t/ha of dry hops compared with the untreated control, while the harvested cones contained the most alpha-bitter acids (4.57%).

Organic farming has become a topic that receives an increasing academic as well as popular attention. This study examines the financial differences between the organic and conventional farmers, as well as their income volatility. Based on the Student's t-test, the regression analysis and the matched-pair investigation on a sample of Czech 291 organic and 4045 conventional farmers over the period 2009-2013, the study indicates that the organic agricultural companies outperform the conventional companies in the terms of profitability. However, their asset turnover is considerably lower. No evidence of different income volatility has been found.

Damage caused by the Goji berry gall mite, Aceria kuko (Kishida, 1927) was observed on the leaves of Lycium chinense Miller (Solanaceae) in Brno in August 2016 and on L. barbarum L. in Popovice in September 2016. Aceria kuko is a pest of Asian origin which is common in China and occurs only sporadically on Goji berry plants in the European Union. This is the first observation of the Goji berry gall mite occurrence in the Czech Republic.

The objective of the present work was to determine changes in total sugars, reducing sugars and sucrose content in potato tubers under conditions involving an application of biostimulants and herbicides. Research material included table potato tubers obtained in a three-year field experiment. The experiment was a split-plot design with three replicates. The factors were as follows: factor I - three table potato cultivars (Bartek, Gawin and Honorata), factor II - five methods of biostimulant and herbicide application (1. control - without biostimulants or herbicides; 2. Harrier 295 ZC (linuron + chlomazon); 3. Harrier 295 ZC (linuron + chlomazon) and growth regulator Kelpak^®SL; 4. Sencor 70 WG (metribuzin); 5. Sencor 70 WG (metribuzin) and growth regulator Asahi^®SL. Variance analysis demonstrated that total sugars, reducing sugars and sucrose content were affected by the biostimulants and herbicides applied as well as cultivars and weather conditions. Compared with control, a significant increase in total sugars followed an application of Sencor 70 WG as well as Sencor 70 WG and the growth regulator Asahi^®SL whereas reducing sugars and sucrose content significantly increased after Sencor 70 WG and the growth regulator Asahi^®SL were applied.

This study evaluated how organic manures and mineral fertilizers affect winter wheat grain and straw yields and grain quality properties. The analysed period of the long-term fertilizer experiment was established in Čáslav, Czech Republic, in 1955 and covers the seasons 2011-2014. The fertilizer treatments were: control; farmyard manure (FYM); FYM + P; FYM + K; FYM + PK; FYM + N₁; FYM + N₂; FYM + N₁PK; FYM + N₂PK and FYM + N₃PK. The highest grain yields were recorded in the FYM + P and FYM + N₃PK treatments (8.9 t/ha). The highest straw yields were recorded in the FYM + N₃PK treatment (6.52 t/ha). The lowest yields were provided in the unfertilized control and FYM treatments. Qualitative parameters were evaluated in the control, FYM and FYM + N₃PK treatments between the years 2011 and 2013. The best quality of wheat grain was provided by the FYM + N₃PK treatment. Combination of the farmyard manure with NPK is the best way to achieve high grain yields with good quality and leads to sustainable food production.

The paper addresses social inclusion. It aims to investigate how social inclusion was conceptualized in the main Czech policy documents related to the EU. The text argues that one of the tools of social inclusion - social economy represented by social entrepreneurship and social farming - is a sort of innovative practice. The findings suggest that the understanding of social inclusion evolves within policy documents towards highlighting social economy through matching its theoretical concept with political measures. However, social economy is still not considered by the documents as an innovative approach. If analysing projects funded under Czech Rural Development Programs, social inclusion in rural areas is not the main theme, especially in regions exposed to the risk of social exclusion. In addition, the main actors (NGOs or social entrepreneurs) who are said to support social economy or social farming are not active in submitting projects in rural areas under the Rural Development Program 2007-2013. These actors have not yet used their potential towards developing social economy.

A prototype of electronic nose (e-nose) with the gas sensor system for evaluation of fresh chicken meat freshness was developed. In this paper a rapid, simple and not expensive system for fresh chicken meat spoilage detection was investigated that provides objective and reliable results. Quality changes in fresh chicken meat during storage were monitored by the metal oxide sensor (MOS) system and compared with the results of traditional chemical measurements. Gas sensor selection was tested for evaluation of volatile fatty acids (VFA) mainly representing meat spoilage.The study demonstrated that a correlation coefficient (R² = 0.89) between e-nose signals and traditional chemical method was high. These results prove that the developed e-nose prototype has a potential for assessing fresh chicken meat freshness and allows discriminating meat into fresh, unsafe and spoiled.

We investigated moth larvae (Lepidoptera) developing in temperate forests in Central Europe shortly after the tree budburst (the "brumata-viridana complex"). Larvae were collected in southern Slovakia in May 2015 and May 2016 from young and mature trees of late flushing Quercus cerris L. and early flushing Q. pubescens Willd. Although Q. cerris yielded fewer species (40 species) than Q. pubescens (47 species), the rarefied number of species and the Chao index suggested a similar number of species on mature trees of both oak species. Both the total number of moth larvae in assemblages and the abundance of dominant species (pests) were significantly lower on Q. cerris than Q. pubescens. The results suggest the release of Q. cerris with delayed budburst from heavy infestations by folivorous moth larvae. Knowledge obtained can be applied in silvicultural and horticultural practices aimed to protect and maintain forest, fruit, and ornamental trees.

Relation between wheat (Triticum aestivum) nutritional status determined at the beginning of stem elongation and during anthesis, respectively, and available content of phosphorus (P-M3) and zinc (Zn-M3) determined by the Mehlich 3 extractant was studied. Both one-year pot experiment with spring wheat and two-year on-farm trials with winter wheat were run on various calcareous soils (pH values of 7.18-7.94, median 7.80, P-M3 1-289 ppm, median 54, and Zn-M3 2-14 ppm, median 4), in the Czech Republic (Central Europe). Phosphorus nutrition index (ratio of phosphorus concentration in shoot biomass to critical phosphorus concentration - P_c) was calculated using the Belanger et al.'s model: P_c = -0.677 + 0.221N - 0.00292N(2), where both phosphorus and nitrogen concentrations were expressed in g/kg shoot dry matter. Unlike phosphorus concentration in shoot biomass, phosphorus nutrition index significantly correlated with P-M3 content in soil. Optimal values of the phosphorus nutrition index were recorded if P-M3 was 51-68 ppm. Zinc concentration in shoot biomass more strongly correlated with P:Zn ratio (M3) in soil compared to Zn-M3 content in soil. P:Zn ratio in shoot biomass of 130:1 did not lead to phosphorus deficiency and corresponded to P:Zn (M3) ratio in soil of 9.3:1-14.3:1.

Spectral data contain information on soil organic and mineral composition, which can be useful for soil quality monitoring. The objective of research was to evaluate hyperspectral visible and near infrared reflectance (VNIR) spectroscopy for field-scale prediction of soil properties and assessment of factors affecting soil spectra. Two hundred soil samples taken from the experiment field (soil depth: 30 cm; sampling grid: 15 × 15 m) were scanned using portable spectroradiometer (350-1050 nm) to identify spectral differences of soil treated with ten different rates of mineral nitrogen (N) fertilizer (0-300 kg N/ha). Principal component analysis revealed distinction between higher- and lower-N level treatments conditioned by differences in soil pH, texture and soil organic matter (SOM) composition. Partial least square regression resulted in very strong correlation and low root mean square error (RMSE) between predicted and measured values for the calibration (C) and validation (V) dataset, respectively (SOM, %: R_C² = 0.75 and R_V² = 0.74; RMSE_C = 0.334 and RMSE_V = 0.346; soil pH: R_C² = 0.78 and R_V² = 0.62; RMSE_C = 0.448 and RMSE_V = 0.591). Results indicated that hyperspectral VNIR spectroscopy is an efficient method for measurement of soil functional attributes within precision farming framework.

This paper describes the process of digitizing Montenegro's legacy soil data, and an initial attempt to use it for digital soil mapping (DSM) purposes. The handwritten legacy numerical records of physical and chemical properties for more than 10 000 soil profiles and semi-profiles covering whole Montenegro have been digitized, and, out of those, more than 3000 have been georeferenced. Problems and challenges of digitization addressed in the paper are: processing of non-uniform handwritten numerical records, parsing a complex textual representation of those records, georeferencing the records using digitized (scanned) legacy soil maps, creating a single computer database containing all digitized records, transforming, cleaning and validating the data. For an initial assessment of the suitability of these data for mapping purposes, inverse distance weighting (IDW), ordinary kriging (OK), multiple linear regression (LR), and regression-kriging (RK) interpolation models were applied to create thematic maps of soil phosphorus. The area chosen for mapping is a 400 km² area near the city of Cetinje, containing 125 data points. LR and RK models were developed using publicly available digital elevation model (DEM) data and satellite global land survey (GLS) data as predictor variables. The digitized phosphorus quantities were normalized and scaled. The predictor variables were scaled, and principal component analysis was performed. For the best performing RK model an R² value of 0.23 was obtained.