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Results 931 to 960 of 2032:

Evaluation of mechanical and combined chemical with mechanical weeding in maize (Zea mays L.), soybean (Glycine max (L.) Merr. and winter wheat (Triticum aestivum L.)Original Paper

Roland Gerhards, Kerstin Hüsgen, Klaus Gehring

Plant Soil Environ., 2024, 70(12):751-759 | DOI: 10.17221/386/2024-PSE

Joint field experiments were established in Southwestern Germany to investigate the potential of herbicide savings on-farm sites with high densities of problematic weed species. From 2020 until 2024, 21 field studies were conducted in maize, soybean and winter wheat, all realised as randomised complete block designs with four replications. Mechanical weeding and two combined chemical with mechanical weeding methods were compared to conventional broadcast pre- and post-emergence herbicide spraying and an untreated control. Weed density, herbicide savings, greenhouse gas emissions and crop yield were determined for all treatments. On average, 142 weeds/m2 were counted in the untreated plots. The most frequent weed species were Chenopodium album, Echinochloa crus-galliSolanum nigrum, Stellaria media, and Veronica persica. Combined chemical with mechanical weed control in soybean and winter wheat was more effective than chemical and mechanical weed control alone. In maize, the combination of hoeing and herbicide application achieved equal weed control efficacy (WCE) as chemical weeding alone. Hoeing removed less intra-row weeds than inter-row weeds. Hoeing and harrowing had low WCE against Chenopodium album and perennial weed species. Combined treatments reduced herbicide use by 24–60% in relation to conventional herbicide treatments. Mechanical and combined weed control achieved equal yield as the conventional herbicide treatment. This study underlines the potential for herbicide savings by integrating mechanical weed control methods.

Robinia pseudoacacia poisoning in two horses: A case reportCase Report

T Novotna, E Samonilova, J Klan, L Frgelecova, A Mala, Z Svobodova, Z Drabkova

Vet Med - Czech, 2024, 69(11):395-400 | DOI: 10.17221/55/2024-VETMED

This case report describes the poisoning of two mares from the same paddock with Robinia pseudoacacia (Black locust) bark. The poisoning manifested itself by the sudden onset of weakness and fever with transient improvement after the administration of non-steroidal anti-inflammatory drugs and fluids. After the initial stabilisation, the mares were left unattended overnight. One of them was found dead in the morning. The surviving mare developed colic and severe encephalopathy and had to be referred to the clinic. Blood tests revealed severe hyperammonaemia. After four days of symptomatic treatment, she fully recovered. Robinia pseudoacacia with bark freshly bitten off was found in the paddock. The necropsy of the dead mare revealed oedema of the brain and lungs, pleural haemorrhages, and hyperaemia and haemorrhages of the glandular mucosa of the stomach. The intestinal content was watery, without macroscopic findings of the bark. Due to the peracute onset in both mares at the same time, hyperammonaemia and paralytic ileus in the surviving mare, and the presence of Robinia pseudoacacia in the paddock, poisoning with this tree was highly suspected. The diagnosis was confirmed by the microscopic findings of Robinia pseudoacacia bark tissue in the gastric content of the dead mare.

Effect of dehulled lupin seeds in feed mixture on muscle protein quality of broiler chickensOriginal Paper

Eva Straková, Lucie V¹etièková, Pavel Suchý, Martin Kutlva¹r

Czech J. Anim. Sci., 2024, 69(12):484-492 | DOI: 10.17221/156/2024-CJAS



The aim of the study was to prepare and test the effect of diets with 50% and 100% replacement of soybean meal with dehulled seeds of white lupin (Lupinus albus) variety Zulika. Two hundred and forty ROSS 308 broiler chickens were divided into control group (C) and two experimental groups E50 (50% replacement of soybean meal with dehulled white lupin meal) and E100 (100% replacement of soybean meal with dehulled white lupin meal). The production parameters and the amino acid spectrum of breast and thigh muscles were evaluated up to 34 days of age. Final live weight of broiler chickens was not significantly affected by the substitution of soybean meal in starter, grower and finisher diets (C 2.3 ± 0.3 kg, E50 2.4 ± 0.3 kg, E100 2.4 ± 0.3 kg). Lupine meal-based diets positively influenced chicken health, which was reflected in a lower mortality rate in the experimental groups (E50 5.0 %, E100 2.5 %) compared to the control group (C 7.5 %). Differences in feed conversion were minimal (C 1.47 kg/kg, E50 1.48 kg/kg, E100 1.45 kg/kg). When evaluating the total amino acid content in the breast and thigh muscle of broiler chickens of the experimental groups (E50, E100), it can be noted that compared to the C group, the total contents of both essential and non-essential amino acids were significantly (P ≤ 0.05) lower in the dry matter of thigh muscle.

Estimation of coffee shelf life under accelerated storage conditions using mathematical models – Systematic reviewReview

María Alina Cueva Ríos, Frank Fernández Rosillo, Lenin Quiñones Huatangari, Eliana Milagros Cabrejos Barrios

Czech J. Food Sci., 2023, 41(2):92-102 | DOI: 10.17221/163/2022-CJFS


The shelf life of a food product is a finite period after manufacturing and packaging, during which it retains a required and acceptable level of quality for consumption. The objective was to characterise and describe the transparent and comprehensible processing process to collect, select, critically evaluate and summarise available evidence regarding the use of mathematical models in the estimation of the shelf life of coffee under accelerated storage conditions. Of the 183 articles identified, nine studies were included in the review: four evaluated various types of packaging containing roasted and ground coffee, three coffee-based beverages, one infusion and one in bean format; the models frequently used were Weibull-Hazard Analysis, first-order, and zero-order kinetic model, which requires the decay kinetic constant, the same as that acquired by the Arrhenius model or a proposed model. Quality descriptors and mathematical models have been identified that allow estimating the shelf life of coffee and its derivative products under accelerated storage conditions, in addition to primary sources with experimental designs.

Effects of controlled irrigation on global warming potential based on CH4, N2O and CO2 fluxes in plateau paddy fieldOriginal Paper

Shufang Wang, Hongchun Bi, Liping Wang, Jing Wang, Ying Wang, Lihong Chen

Plant Soil Environ., 2024, 70(9):535-542 | DOI: 10.17221/453/2023-PSE

A suitable irrigation pattern is of great significance for reducing greenhouse gas emissions. In this study, field experiments and a denitrification-decomposition (DNDC) model were used to study the global warming potential based on CH4, N2O and CO2 fluxes under flooding irrigation and controlled irrigation in paddy fields in the Erhai Lake basin. The results showed that the average value of CH4 flux under controlled irrigation was lower than that under flooding irrigation, with a reduction range of 43.21% to 48.88%, however, the average value of the N2O and CO2 fluxes from paddy field under controlled irrigation were higher than those under flooding irrigation. Controlled irrigation patterns can significantly reduce the global warming potential in paddy fields based on CH4, N2O and CO2 fluxes. Controlled irrigation can effectively reduce the global warming potential per unit yield. For water management in the Erhai Lake basin, it is recommended the controlled irrigation treatment of soil moisture with an upper limit of 100% and a lower limit of 75–85% with irrigation, and a maximum surface water depth of 150–200 mm lasting for five days after precipitation from the jointing-booting stage to the milk stage.

Meta-analysis of fluorochrome concentrations to assess bull sperm quality by flow cytometry in 2021–2023Review

Filipp Georgijeviè Savvulidi, Martin Ptáèek, Szabolcs Nagy, Ludìk Stádník

Czech J. Anim. Sci., 2023, 68(12):469-485 | DOI: 10.17221/152/2023-CJAS

Flow cytometry (FC) is a very powerful method for the assessment of spermatozoa quality in multiple livestock species, including bulls. Nowadays, many flow cytometric tests are available for quality detection of bull sperm. These tests are based on fluorochromes (fluorescent tags or dyes) and the use of correct concentrations of fluorochromes is an essential aspect of the successful optimization of experimental methodology. Modern flow cytometers are much more affordable, such that spermatologists are now able to use FC methodology (and even image cytometry) in their studies. The present review aims to give a summary of current flow cytometric methodologies used to assess bull sperm quality by flow cytometry. Namely, we give here the concentrations of fluorochromes used in the studies related to bull sperm quality evaluation by flow cytometry published in the last three years (from 2021 to 2023). Importantly, in the present review, the concentration of fluorochromes is unified and presented using comparable molarity units. Furthermore, the rationale of each flow cytometric methodology for particular fluorescent tests, and the citation for the corresponding original methodology, are given. This review will help spermatologists shorten the time needed for the optimization of their flow cytometry methodology for the assessment of bull sperm quality.

Double-wire woven nitinol stent for treating dogs with refractory tracheal collapse: A case seriesCase Report

JY Choi, Kim MY, HY Yoon

Vet Med - Czech, 2024, 69(1):30-34 | DOI: 10.17221/61/2023-VETMED

This retrospective case series describes the signalments, clinical signs, diagnostic test results, and postoperative complications obtained from the medical records of 10 client-owned dogs that underwent treatment for grade IV tracheal collapse using double-wire woven nitinol stents between October 2017 and September 2021. Respiratory signs resolved in all dogs immediately after tracheal stent placement. Mild to moderate stent fractures were identified in five dogs, of which two showed concurrent respiratory distress necessitating re-stenting after several months. Minor complications, such as stent migration, were absent. The double-wire woven nitinol stent optimised for the canine trachea showed favourable outcomes and minimal complications.

The relationship of soil sulfur with glomalin-related soil protein and humic substances under different mineral and organic fertilisationOriginal Paper

Pavel Suran, Jiøí Balík, Martin Kulhánek, Ondøej Sedláø, Jindøich Èerný

Plant Soil Environ., 2024, 70(2):93-100 | DOI: 10.17221/493/2023-PSE

In recent years, sulfur inputs into the soil have greatly diminished due to the significant decrease in SO2 emissions. Plant nutrients, like sulfur, can be released by the mineralisation of soil organic matter (SOM), which is a complicated mixture of substances (or fractions) like glomalin-related soil protein (GRSP) and fulvic acids (FA), humic acids (HA), humic substances (HS) and others. GRSP, FA, HA, and HS content, as well as the content of mineral and organic fractions of sulfur, was determined in different mineral and organic fertiliser treatments of the long-term field experiment. Using these results, the sulfur content in GRSP was calculated based on the soil’s organic matter carbon and soil’s organic bound sulfur (CSOM/SORG) ratio. Sulfur content in GRSP was 4.08–5.46 (easily extractable GRSP), 9.77–15.7 (difficultly extractable GRSP), and 13.9–21.1 (total GRSP) mg S/kg of soil. Overall, the application of the organic fertiliser caused an increase in S content bound to GRSP. A strong significant relationship was also observed between GRSP fractions and soil organic sulfur. A similar relationship was also observed for the HA and HS with organic sulfur.

Sex ratio dynamic of the field population of the sugarcane rust mite Abacarus sacchari (Acari: Eriophyidae)Original Paper

Nur Asbani, Hardev Sandhu, Oscar Liburd, Julien Beuzelin, Ronald Cherry, Gregg Nuessly

Plant Protect. Sci., 2024, 60(1):89-96 | DOI: 10.17221/50/2023-PPS

Sugarcane in southern Florida suffers from sugarcane rust mite (SRM) infestations, Abacarus sacchari, from summer to early fall. The mite’s sex ratio is important in understanding the mite's demography and population dynamics. This is the first report on the sex ratio of the field population of the SRM. The objectives of the study were to determine the sex ratio of the SRM and the factors that affect any changes in the sex ratio. To determine the sex ratio, mites were sampled from a sugarcane canopy each month over a 12-month period and the sex ratio was expressed as the proportion of females to the total number of sexed mites. The population density and aerial mites were monitored weekly for 8 and 6 months, respectively. The total number of sexed mites from the sugarcane canopy was 27 941 mites, while 2 248 airborne mites were recorded. The result showed that the sex ratio of the SRM in the canopy was dynamic during the study, with a female bias more common than a male bias among the samples, which ultimately resulted in a slightly female bias. An obvious change from a female bias to a male bias occurred simultaneously with the increased mite density and dispersal. The factors affecting the oscillation of the sex ratio are discussed.

Cup viscometer – A practical analytical toolShort Communication

Milan Hou¹ka

Czech J. Food Sci., 2024, 42(1):71-76 | DOI: 10.17221/159/2023-CJFS


This paper focuses on helping smaller companies in the manufacturing industry who need to know the viscosity of their products or raw materials and do not have the funds to invest in expensive rotary rheometers. We developed a calibrated simple cup. It has replaceable nozzles designed so that dripping liquids cannot distort discharge times. The calibration of the cup is based on the relationship between kinematic viscosity and outflow time, which is valid for the laminar flow regimes. Here we described calibration and measurement procedures and methods for detecting non-Newtonian flow behaviour and the viscoelasticity of measured liquids. The maximum measurement error of 3% is defined for cases where all distractions are removed.

Reconstructed military machine for unique field testing of agricultural machinery capabilitiesOriginal Paper

Kornél Szalay, Jiøí Souèek, Gábor Bércesi, Adrienn Bablena, Kovács Lászlo, Sayakhat Orazovich Nukeshev, Savelii Kuharets, Volodymyr Kravchuk, Gennadii Golub, Antonín Machálek, Aleksandr Vladimirovich Dobrinov

Res. Agr. Eng., 2024, 70(1):53-59 | DOI: 10.17221/60/2023-RAE

Detailed performance testing of agricultural machinery is very important in determining its value in use. These measurements cannot be limited to laboratory tests, such as braking the power take-off (PTO) shaft of  a tractor or performing dynamic tests of implements in a soil bin. Field tests are the ultimate way to test the capabilities of agricultural machinery. However, during such field tests, there are many parameters that can interfere with and affect the test results, such as inhomogeneity of the soil and tyre characteristics. In order to minimise these effects as much as possible, Hungarian University of Agriculture and Life Sciences (MATE) Institute of Technology, Agriculture Engineering Labs. has developed a dynamometer vehicle with an electronic brake control system that is suitable for measuring the traction characteristics of agricultural tractors and other terrain vehicles. It  is also capable of testing different track systems and tyre-soil interactions. This paper introduces this special test vehicle by presenting measurement results and also describes other interesting applications for the agricultural community.

Farm size and greenhouse gas emission: Do large farms in China produce more emissions?Original Paper

Zhe Zhao, Fan Zhang, Yiqiong Du, Xin Xuan, Ying Cai, Gui Jin

Agric. Econ. - Czech, 2024, 70(3):112-124 | DOI: 10.17221/307/2023-AGRICECON

Farms are key to agricultural advancement and carbon emission reduction. Understanding the influence of farm size on emissions is vital for eco-friendly farming. Our study used an econometric model with instrumental variable adjustments to examine the effect of farm size on greenhouse gas emissions, revealing an inverted U-shaped relationship. The findings revealed that emissions increased with farm size until a peak and then decreased. We identified an optimal farm size range (0.45 km² to 0.58 km²) for lower emissions, where the farm size maintaining the lowest greenhouse gas emissions per unit area was 0.58 km², while the lowest greenhouse gas (GHG) emissions per capita occured at a farm size of 0.69 km². Reducing emissions intensity per unit area is easier than reducing GHG emissions per person. Policymakers should prioritise promoting the expansion to moderately sized farms as a means of achieving emission reduction targets rather than solely increasing the number of farms. Overall, these insights offer policymakers novel approaches for ecological farm planning and the transition toward a low-carbon agriculture sector.

Field study: Factors influencing virgin queen bee acceptance rate in Apis mellifera coloniesOriginal Paper

Agnese Smilga-Spalvina, Kriss Spalvins, Ivars Veidenbergs

Czech J. Anim. Sci., 2024, 69(4):155-164 | DOI: 10.17221/22/2024-CJAS

Regular replacement of old and problematic queen bees is necessary to maintain the strength and productivity of bee colonies. Beekeepers replace queen bees using queen cells, virgin queens, or mated laying queens. In this study, we explored factors influencing the acceptance of the virgin queens. A comprehensive field study was conducted in the summer of 2023, involving the introduction of 754 virgin queens of different age and 194 queen cells into mating nuclei of various states using three different methods. The article aims to provide recommendations to beekeepers for introducing virgin queens, thereby increasing the frequency of successful requeening. The duration of the colony being queenless has a significant impact on the acceptance of queens. If the colony is prepared in advance, at least five days prior, immature and mature virgin queens will be accepted equally well (60‒77%). Conversely, suppose queen replacement is done without preparation for a colony that has been queenless for one day only. In that case, the result will be significantly lower due to the short queenless period (41%), and the age factor of queen bees will come into play. If queen replacement is done with preparation, the chosen timing within the season will play a significant role, as weather conditions can influence acceptance and mating, varying from 48% to 89%. Suppose a bee colony has long lost its queen and has developed laying worker bees. In that case, there is still a 55% chance of successfully introducing a virgin queen into such a colony without using time-consuming methods.

Mammary neoplasms in female dogs: Clinical, diagnostic and therapeutic aspectsReview

JR Rueda, CD Porto, RP Franco, IB da Costa, LMC Bueno, RJS Girio, FFR Manhoso, PCS Bueno, CSF Repetti

Vet Med - Czech, 2024, 69(4):99-114 | DOI: 10.17221/4/2024-VETMED

With the increase in the life expectancy of domestic animals and their increasingly affectionate relationship with their owners, it is possible to observe an increase in cases of neoplasms in these animals. Mammary neoplasia mainly affects older females who have not been castrated, due to hormonal dependence for the development of the tumour. The main form of treatment is surgery. This study aims to carry out an updated review on mammary neoplasms in female dogs covering the anatomy, physiology, prevalence, causes, diagnoses, treatments, prevention and prognosis, based on scientific articles by renowned researchers.

Physiological and metabolic responses of Lolium perenne L. roots to acid stress in cadmium-contaminated soilOriginal Paper

Xingrong Bai, Lili Chen, Zhaojie Wang, T. Ryan Lock

Plant Soil Environ., 2024, 70(6):366-376 | DOI: 10.17221/494/2023-PSE


Perennial ryegrass (Lolium perenne L.) has potential in the phytoremediation of cadmium (Cd)-contaminated soil due to its strong Cd accumulation capacity and high biomass. In this study, we investigated the growth physiology, Cd accumulation, and metabolites of L. perenne roots under different soil acid stress levels (pH 4.0, 4.5, 5.0 and 6.0) and Cd concentrations (100 and 0 mg/kg) after 90 days of growth. The results showed that soil acid stress significantly impacts the remediation capability and physiological metabolic properties of L. perenne. Based on root Cd content and enrichment coefficient, soil pH between 4.5 and 5.0 was more conducive to Cd accumulation. The growth physiology and Cd accumulation of L. perenne were inhibited under high soil acid stress (pH 4.0). High soil acid stress caused a decrease in root length, root volume, and root biomass of L. perenne. Root malondialdehyde (MDA) content and the activity of antioxidant enzymes (catalase (CAT) and peroxidase (POD)) increased significantly in response to high soil acid stress to enhance tolerance. Metabolomics analysis revealed that acid stress resulted in significant changes in certain metabolites. Tartaric acid, fructose and amino acids (glutamate and lysine) in the roots of L. perenne were compatible solutes under acid stress. This study indicated that L. perenne has strong physiological and metabolic tolerance, as well as Cd accumulation ability, in response to soil acid stress.

Application of the physical properties of local cowpea varieties in the development of a multi-variety cowpea cleanerOriginal Paper

Babatunde Olusola Adetifa, Elizabeth Ayobami Olumomi, Taiwo Moses Samuel, Ayoola Abiola Babalola

Res. Agr. Eng., 2024, 70(2):82-91 | DOI: 10.17221/51/2023-RAE

Cowpea, an annual legume widely grown and consumed in Nigeria, has been observed to contain between 27–33% impurities when freshly harvested and threshed. This poses a threat to humans when consumed and in large-scale agricultural processing. Therefore, this study is aimed at developing and evaluating the performance of a multi-variety cowpea cleaner (MVCC). Using standard methods, some selected engineering properties of the cowpea varieties were examined and used in the design of the MVCC. The cowpea had a moisture content of 8–14%, depending on the locations and varieties. Other properties investigated include the length, width, thickness, sphericity, geometric mean diameter, unit volume, arithmetic mean diameter, aspect ratio, surface area, unit weight, true density, terminal velocity, and angle of repose. The MVCC comprised the hopper, winnower, cleaning unit, fan assembly, and frame. The performance of the MVCC was also evaluated. The efficiency of separating good products of honey, drum, and Sokoto White beans was 95, 91, and 84%, respectively, while separating bad products was 87, 94, and 96%, respectively.

Enhanced maize yield and nitrogen efficiency with low molecular weight fulvic acid: insights into chlorophyll a/b ratio and nitrogen metabolising enzyme activityOriginal Paper

Fugui Li, Shujie Zhang, Longhang Chai, Zhiqiang Guo, Peipei Li, Yanlai Han, Yi Wang

Plant Soil Environ., 2024, 70(10):632-643 | DOI: 10.17221/320/2024-PSE

This study investigates the impact of various molecular weights (MWs) of fulvic acid (FA) on maize growth, grain yield, and nutrient uptake under different nitrogen levels (NLs). A 2 × 3 balanced design was employed, with high (0.2 g N/kg) and low (0.05 g N/kg) NLs, and three FA MW ranges (W1 ≤ 3 000 D, 3 000 < W2 ≤ 10 000 D, W3 > 10 000 D) were applied at 25 mg/kg in soil. Significant interactions between NLs and FA MWs were observed in chlorophyll a/b ratio, nitrate reductase and glutamate dehydrogenase activities, nitrogen content, and nitrogen uptake efficiency. Overall, under different NLs, FA application reduced chlorophyll a/b ratio, increased nitrogen metabolism enzyme activities, promoted maize growth, and thereby improved grain yield and nitrogen fertiliser uptake efficiency. Additionally, the promotion effect of low MW FA on these indicators outweighed that of high MW FA, yet the latter exhibited a more pronounced effect on increasing grain nitrogen concentration. Structural equation model analysis revealed direct effects of chlorophyll content, nitrogen accumulation, nitrogen uptake efficiency, NLs and FA MWs on maize grain yield, with FA MWs negatively impacting yield.

Rhizosphere and non-rhizosphere soil organic carbon and its labile fractions in alpine desertified grassland affected by vegetation restorationOriginal Paper

Chuanyu Zhou, Hongyu Qian, Aiyang Liu, Yufu Hu, Wei Wang, Gang Chen, Zhi Li

Plant Soil Environ., 2024, 70(8):523-533 | DOI: 10.17221/106/2024-PSE

Grasslands are the predominant land use type in China, which is currently encountering significant desertification issues. Consequently, restoring grassland vegetation has important implications for terrestrial carbon (C) levels and, consequently, the global C balance. This study focused on Salix cupularis, the primary plant used for desert control on the eastern edge of the Qinghai-Tibet Plateau. We analysed the rhizosphere and non-rhizosphere soil up to the depth of 60 cm after Salix cupularis growth for 0–24 years, examining soil total organic carbon (TOC) and its labile fractions. Following restoration, there was a gradual increase in TOC and its labile fractions, with the most significant changes observed in the rhizosphere soil at a depth of 0–20 cm. After 24 years of restoration, the TOC content in both rhizosphere and non-rhizosphere soil had increased by 141.74% and 39.44%, respectively. Labile organic C in the rhizosphere soil increased more rapidly and pronouncedly compared with the TOC. Specifically, dissolved organic C and easily oxidised organic C in the rhizosphere soil saw substantial increases of 211.03% and 217.65%, respectively. Meanwhile, compared with the 4 years of restoration, soil C pool management index of the 8–24 years soils increased, ranging from 15.70% to 132.21%. Therefore, long-term vegetation restoration on the eastern margin of the Qinghai-Tibet Plateau can significantly enhance TOC and its labile fractions, as well as improve soil C sink capacity and quality.

How the milk chemical composition and fatty acid profile are influenced by physiological factorsin Najdi dairy sheepOriginal Paper

Abdulkareem Mohammed Matar, Faisal Ahmed Alshamiry, Ramzi Ahmed Amran, Riyadh Salah Aljumaah, Muneeb Musthafa, Massimo Todaro

Czech J. Anim. Sci., 2024, 69(7):280-291 | DOI: 10.17221/37/2024-CJAS


Sheep milk production is a complex process that is influenced by various factors. This study aims to investigate how the litter size (single vs. twins), birth type (male vs. female), age of the ewes and body weight affect the milk composition and fatty acid profile of milk fat. Milk samples were collected from 119 Najdi ewes that were the subject of this study. Gas chromatography-mass spectrometry (GC-MASS) was used to quantify the fatty acids (FAs). The results showed that the twins birth type (female/female) has a significant influence (P < 0.05) on the concentration of linoleic acid (LA), alpha-linolenic acid (ALA), behenic acid (C22:0) and polyunsaturated fatty acid (PUFA). On the other hand, saturated fatty acids (SFA), docosahexaenoic acid (C22:4; DHA), and odd-chain fatty acids (OCFA), such as C15:0-antiso and C19:1-cis 10, increased significantly (P < 0.05) with the increasing age and body weight of the ewes, while the ALA and unsaturated fatty acids (UFA) significantly decreased (P < 0.05). The principal component analysis (PCA) revealed a positive association between the age and the OCFA, ALA and small-chain fatty acids (C6:0 and C8:0). In addition, the type of birth showed a positive association with the fat, lactose and palmitoleic acid C16:1 cis9. Conversely, there is a negative association between the UFA, monounsaturated fatty acids (MUFA) and PUFA. In addition, the body weight (BW) and litter size were negatively associated with the protein, SFA and medium-chain fatty acids (C10:0, C12:0, C14:0 and C16:0). The physiological factors generally suggested that the milk quality and essential FA, such as ALA, were influenced by the type of the lamb’s birth and the age of the ewes.

Effect of rosemary and clove essential oils on lipid oxidation, microbial, sensorial properties and storage stability of kavurma, a cooked meat productOriginal Paper

Sezen Harmankaya, Ahmet Harmankaya, Koray ݺbarali, Özlem İstanbullu Paksoy

Czech J. Food Sci., 2024, 42(4):251-262 | DOI: 10.17221/56/2024-CJFS


Kavurma is a traditional cooked meat product of Türkiye containing a high percentage of animal fat (30–40%). Therefore, kavurma can undergo lipid oxidation easily. This may cause a major problem in the storage and marketing of the product. Synthetic additives used in foods cause anxiety in consumers and this situation increases the search for natural alternatives. Plant essential oils can be reliable additives to extend the shelf life of foods. The protective effect of rosemary and clove essential oils on kavurma meat against microbiological and oxidative deterioration was investigated in this study. For this purpose, kavurma samples were divided into five groups after being produced by standard methods, the essential oils (rosemary and clove) obtained by distillation method were added to the kavurma fat in various proportions, alone or in combination. At the end of the study, the thiobarbituric acid-reactive substance (TBARS) and peroxide values of the groups containing plant essential oils were found to be lower than the control groups. The groups containing rosemary and clove essential oil showed that microbiological deterioration was delayed and, the sensory evaluation scores were higher than the control groups at the end of the cold storage period.

Effects of mineral/organic fertilisation on the soil organic nitrogen pool in a semi‑arid steppeOriginal Paper

Dongqi Jiang, Nan Jiang, Zhuo Wang, Chenran Wu, Lijun Chen, Yulan Zhang, Zhenhua Chen

Plant Soil Environ., 2024, 70(11):695-701 | DOI: 10.17221/160/2024-PSE

Compared to solely mineral fertiliser application, organic fertiliser substitution has been demonstrated to be effective in enhancing nitrogen cycling in cropland, but the relevant research based on grassland is still insufficient. A field experiment was carried out in the grasslands of northern China to examine the impact of mineral/organic fertilisation on soil organic nitrogen fractions, activities of soil N-hydrolysing enzymes and their interrelationships. The results showed that the active soil organic nitrogen pool (hydrolysable NH4+-N + amino sugar-N + amino acid N) increased significantly after fertilisation, and organic fertiliser combined with mineral fertiliser addition showed the best promotion effect. N-acetyl-β-d-glucosaminidase, protease and urease activities are also significantly affected by fertilisation. The findings of our study indicate that the combination of mineral fertiliser and organic fertiliser shows a higher potential in improving the active soil organic nitrogen supply. This approach seems to be a viable agronomic strategy for augmenting soil nitrogen supply and ensuring the stability of the soil nitrogen pool in the semi-arid steppe region of northern China.

Innovative approach to utilising magnetic fields to enhance wheat yield: evidence from field studies in ChinaOriginal Paper

Yinglei Zhang, Hangyu Dou, Liuyang yang, Yihan Zhang, Wei Sun, Yihao Ruan, Jiameng Guo, Yongchao Wang, Ruixin Shao, Qinghua Yang, Hao Wang

Plant Soil Environ., 2024, 70(10):656-672 | DOI: 10.17221/285/2024-PSE

Magnetic fields, as a form of physical energy, exert an influence on biological activities. However, our current understanding of the impact of magnetic fields on wheat yield remains limited. In this study, our objective was to investigate the effects of magnetic field treatment of wheat plants on their yield, root growth, absorption of nitrogen and phosphorus and soil bacterial diversity. The experiments were conducted at two agricultural research stations in China, Zhengzhou and Xuchang. Plants were treated with magnetic fields of 20, 40, 60, and 80 mT induced by permanent magnets for chronic exposure. Untreated plants were considered as controls. Our result showed that soil nutrients were found to have a substantial impact on wheat nitrogen and phosphorus absorption, and wheat nitrogen and phosphorus absorption significantly affected wheat yield. The change in soil nutrient content was caused by the change in soil bacterial community diversity and abundance, and increased soil nutrients increased wheat yield. The results suggest that magnetic field treatment stimulated wheat plant growth and yield, and changed soil nutrient content through improved soil bacterial community diversity and increased soil nitrogen and phosphorous absorption.

Does a relation between bone histomorphometry and fractures exist? The case of the equine radius and tibiaOriginal Paper

M Zedda, R Babosova, S Gadau, G Lepore, S Succu, V Farina

Vet Med - Czech, 2024, 69(9):307-313 | DOI: 10.17221/18/2024-VETMED

Fractures of long bones in limbs are rare traumatic events in horses. This study investigates whether the incidence and types of fractures can be related to the histomorphometric features of the radius and tibia, which experience different biomechanical stresses and exhibit varying incidences and types of fractures. Clinical observations suggest that, in adults, slightly transverse and comminuted fractures are present in the radius, while the tibia shows a higher frequency of longitudinal and spiral fractures. Microscopic observations reveal no apparent distinctive characteristics between the radius and tibia, whereas the histomorphometric data highlight differences in the osteon density, eccentricity, and diameters of the osteons and Haversian canals. To sum up, tibial osteons are more numerous and smaller than those in the radius, resulting in a 15% higher total extension of the cement line in the tibia compared to the radius. These histomorphometric differences are an evolutionary adaptation to the different biomechanical stresses that involve the thoracic and pelvic limbs. Our results could help better understand numerous clinical realities detectable through retrospective analyses and aid in evaluating a specific bone’s predisposition towards traumatic events in all mammals, including humans.

Rediscovery of tospovirus vector Dictyothrips betae (Thysanoptera: Thripidae) in Slovakia after 72 years – Short CommunicationShort Communication

Michaela Me¹ková, Martina Zvaríková, Rudolf Masaroviè, Jozef Balcerèík, Peter Fedor

Plant Protect. Sci., 2024, 60(4):407-410 | DOI: 10.17221/106/2024-PPS

The new record of Dictyothrips betae Uzel, 1895 (Thysanoptera: Thripidae) after 72 years in Slovakia is presented. Dictyothrips betae is an important pest and one of the few thrips species known as vectors of dangerous plant tospoviruses, causing severe yield losses to economically important crops worldwide. D. betae was swept from an herbal layer in blown dunes in SW Slovakia.

Optimisation of the inhibitory effect of Lactiplantibacillus plantarum, nisin, and lysozyme to prevent the late blowing defect in a cheese modelOriginal Paper

Sinan Akbal, Zübeyde Öner

Czech J. Food Sci., 2024, 42(5):330-339 | DOI: 10.17221/78/2024-CJFS


The present study employed response surface methodology (RSM) to optimise the prevention of late blowing defects in cheese during storage. The aim was to enhance the inhibition of Clostridium sporogenes in cheese by manipulating three independent variables: Lactiplantibacillus plantarum (utilising two different strains, labelled as A and B, the total cheese inoculation rate was 2% overall and the A : B ratio ranging from 25% to 75%), and lysozyme (ranging from 0 to 0.2 mg·L–1). The response variables considered in this model cheese study included the Clostridium count, pH, and titratable acidity. The results showed that the optimal conditions for inhibiting C. sporogenes and preventing late blowing defects in cheese were achieved with an L. plantarum A : B ratio of 49.54 : 50.46%, nisin at a concentration of 1.762 mg·L–1, and lysozyme at 0.2 mg·L–1. These results demonstrated not only effective inhibition of C. sporogenes, a pivotal contributor to late blowing defects in cheese but also indicated favourable outcomes in terms of acidity parameters, which are crucial quality criteria for cheese production. The application of Response Surface Methodology revealed that late blowing defects can be prevented using relatively lower concentrations of antimicrobial agents, along with a judicious selection of appropriate cultures. This research highlights the potential for more efficient and cost-effective strategies to maintain cheese quality by minimising the risk of late blowing defects.

Market entry strategy and export destination in the Spanish wine industryOriginal Paper

Raul Serrano, Juan Ramón Ferrer, Silvia Abella, Vicente Pinilla

Agric. Econ. - Czech, 2024, 70(12):621-632 | DOI: 10.17221/107/2024-AGRICECON

This paper analyses the market entry strategy of the Spanish wineries and their destination markets. For this purpose, channel adjustment was analysed. The novel aspect of this research is the use of exports on a winery level, basing the explanation of their export performance on a combination of internal decisions of the companies, and characteristics of the wine destination markets, in accordance with the purchasing patterns of their consumers. The results revealed the importance of strategic adjustment between winery and market destination, even though the importance of adjustment depends on the level of exports. Thus, channel adjustment is important for the probability of export and export intensity.

The effect of mechanical site preparation on sandy soil properties in Scots pine plantationsOriginal Paper

Ján ®idó, Milan Ka¹iar, Marián Homolák, Erika Gömöryová

J. For. Sci., 2024, 70(11):593-601 | DOI: 10.17221/54/2024-JFS

Sandy soils represent an extreme environment for tree growth. Traditionally, site preparation before planting involves removing logging residues (LRR) and ploughing. An alternative method is incorporating logging residues (LRI) into the topsoil which may enhance tree regeneration and seedling growth. The aim of this study was to assess whether and how different site preparation techniques affect soil physico-chemical and microbial properties over the long term. The study was performed in the Záhorská ní¾ina lowland (Slovakia) in September 2020. Soil samples were taken in two 25-year-old Pinus sylvestris (L.) plantations along five soil profiles in each stand, down to a depth of 30 cm. Results showed a significant increase in carbon and nitrogen concentration and soil moisture in the LRI plot. However, soil pH and phosphorus content significantly decreased. No significant differences were observed in calcium, magnesium, and potassium concentrations between the differently treated plots. The LRI plot also exhibited a significant increase in microbial biomass carbon, N-mineralisation, and catalase activity. The results indicate that different mechanical site preparation methods may impact soil properties over the long term, likely through improved seedling survival and tree growth.

Comparison of phenylpropanoid metabolism of three physiological disorders in apple and pearOriginal Paper

Linru Wang, Gengsen Liu, Lianmei Liu, Yugang Zhang, Haiyong Qu

Hort. Sci. (Prague), 2024, 51(4):278-285 | DOI: 10.17221/103/2023-HORTSCI

Apples and pears are the main temperate fruit tree species, and metabolic disorders may occur during their development and post-harvest storage periods, leading to physiological diseases. In this study, we detected the phenylalanine ammonia-lyase (PAL) enzyme and related metabolites involved in phenylpropanoid metabolism in the pulp of pear with hard-end or cork spot and in the pulp of apple with bitter pit. These three physiological disorders differed in phenylpropanoid metabolism and had similar PAL activity, but their polyphenol, flavonoid, and lignin contents changes were completely. For fruits with the three types of physiological metabolic disorders, the auxin content in the tissues with metabolic disorder symptoms was higher than that in the healthy tissues. In summary, the three physiological disorders showed similar changes in the activities of key enzymes (i.e., PAL) involved in phenylpropane metabolism, but their metabolites significantly differed.

Selection of trees for rubbing by the wild boar (Sus scrofa) in the Sidi Boughaba forested Moroccan Ramsar site: Assessment, implications, and perspectivesOriginal Paper

Abdellah Ichen, Saâd Hanane, Mohammed Bouaamama, Mohamed Alaoui, Najib Magri, Abdelaziz Benhoussa

J. For. Sci., 2023, 69(1):11-20 | DOI: 10.17221/139/2022-JFS


Knowledge of mechanisms by which large mammals select rubbing trees (RT) is a major challenge for the effective management of forests and wildlife resources. In this study, we investigated this issue regarding the Wild boar (Sus scrofa) in the Moroccan forested site of Sidi Boughaba as a case study. We used data from four sets of variables, namely topography, forest type, landscape composition, and microhabitat, measured at 58 rub and control trees, to determine the factors associated with the occurrence of RT by means of generalized linear mixed models. Our results showed that the RT occurrence increased with a high density of red juniper trees and declined with distance to the nearest footpath. Variation partitioning analysis revealed that the pure fraction of microhabitat was the most robust in explaining this occurrence (adj. R2 = 0.17, P < 0.001), followed by that of forest type (adj. R2 = 0.05, P < 0.05). A scientific monitoring system must be set up to strike a balance between the availability of forest trees on the one hand and the pressure exerted by wild boars on this internationally importance site on the other. It is imperative to test the geographical generality of our results in other Mediterranean forests.

Influence of soil tillage technology on tillage erosionOriginal Paper

Jaroslav Korba, Pavel Bro¾, Josef Hùla, Petr Novák, Václav Novák

Res. Agr. Eng., 2024, 70(2):104-110 | DOI: 10.17221/64/2023-RAE


Tillage-induced erosion has negative impacts on the soil environment and production of the soil under intensive farming. Tillage erosion was evaluated during soil tillage performed by two technologies, i.e. conventional tillage and reduced tillage, commonly used in the Czech Republic. A field experiment was aimed at evaluating the soil particle translocation and magnitude of the vector angle. Aluminium cubes with an edge length of 16 mm were used as tracers. After each soil tillage operation, a metal detector searched these tracers in the topsoil. During the experiment, agricultural practices were always carried out on their respective dates for the whole season. The experiment results show that conventional tillage had a more adverse effect on tillage erosion than reduced tillage. This was confirmed on three experimental parcels with different slope gradients of 2, 6 and 11°. The largest translocation of soil tracers was observed on a parcel with the highest slope of 11°. There, the length of the translocation of tracers reached up  to almost 10 m. The average length of soil tracer translocation in reduced tillage and conventional tillage ranged between 0.86 and 3.69 m. The largest average vector angle of tracer locations was recorded on a parcel with a slope of 6° for reduced tillage. In the treatment with the slope of 2° and conventional tillage used, the direction vector indicated upslope translocation of soil tracers. It was caused by soil tillage with a mouldboard plough turning over the topsoil layer upslope. In a treatment with a slope of 2° and reduced tillage used, no influence of the crosswise slope gradient of the plot on the direction vector was observed. The acquired knowledge will be used in further study of soil erosion processes.

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