Effect of semen extender supplementation with sericin on post-thaw dairy bull sperm quality and lipid peroxidation

https://doi.org/10.17221/104/2020-CJASCitation:

Yangngam Y., Chapanya S., Vongpralub T., Boonkum W., Chankitisakul V. (2021): Effect of semen extender supplementation with sericin on post-thaw dairy bull sperm quality and lipid peroxidation. Czech J. Anim. Sci., 66: 13–20.

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Silk sericin plays a protective role in a variety of mammalian cells during cryopreservation. This study aimed to determine the effects of sericin supplementation to a freezing extender on sperm quality and lipid peroxidation in dairy bull sperm cryopreservation. Each semen sample of five Holstein-Friesian crossbred bulls was divided into four aliquots and diluted in a tris-egg yolk extender supplemented with different concentrations of sericin [0%, 0.25%, 0.5%, and 1.0% (wt/vol)]. Sperm motility (CASA), viability, acrosome integrity, mitochondrial membrane potential (fluorescent staining) and lipid peroxidation (malondialdehyde – MDA test) were analysed. The results show that the 0.25% and 0.5% sericin groups had the highest total sperm motility (P < 0.05). Sperm viability, acrosome integrity, and mitochondrial function were higher in the group supplemented with 0.25% sericin compared to the control and 1.0% (P < 0.05). Sericin supplementation with 0.25% and 0.5% significantly decreased MDA concentrations compared with the control (P < 0.05). In conclusion, supplementation of the semen freezing extender with sericin at the concentration of 0.25% significantly improved the post-thaw semen quality and reduced lipid peroxidation in Holstein-Friesian crossbred bulls.

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Kefer JC, Agarwal A, Sabanegh E. Role of antioxidants in the treatment of male infertility. Int J Urol. 2009;16(5):449-57.  https://doi.org/10.1111/j.1442-2042.2009.02280.x
 
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Losano JDA, Angrimani DSR, Rui BR, Bicudo LC, Dal-mazzo A, Silva BCS, Viana CHC, Mendes CM, Assumpcao MEOA, Barnabee VH, Nichi M. The addition of docosahexaenoic acid (DHA) and antioxidants (glutathione peroxidase and superoxide dismutase) in extenders to epididymal sperm cryopreservation in bulls. Zygote. 2018 Jun;26(3):199-206.
 
Lv C, Larbi A, Wu G, Hong Q, Quan G. Improving the quality of cryopreserved goat semen with a commercial bull extender supplemented with resveratrol. Anim Reprod Sci. 2019 Sep;208:106-27.
 
Kumar P, Kumar D, Sikka P, Singh P. Sericin supplementation improves semen freezability of buffalo bulls by minimizing oxidative stress during cryopreservation. Anim Reprod Sci. 2015 Jan;152:26-31.
 
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Lange-Consiglio A, Meucci A, Cremonesi F. Fluorescent multiple staining and CASA system to assess boar sperm viability and membranes integrity in short and long-term extenders. Open Vet J. 2013 Mar;3(1),21-35.
 
Budai C, Egerszegi I, Olah J, Javor A, Kovacs A. The protective effect of antioxidants on liquid and frozen stored ram semen. Sci Pap Anim Sci Biotechnol. 2014 May 31;47:46-52.
 
Losano JDA, Angrimani DSR, Rui BR, Bicudo LC, Dal-mazzo A, Silva BCS, Viana CHC, Mendes CM, Assumpcao MEOA, Barnabee VH, Nichi M. The addition of docosahexaenoic acid (DHA) and antioxidants (glutathione peroxidase and superoxide dismutase) in extenders to epididymal sperm cryopreservation in bulls. Zygote. 2018 Jun;26(3):199-206.  https://doi.org/10.1017/S0967199418000096
 
Mandal R, Badyakar D, Chakrabarty J. Role of membrane lipid fatty acids in sperm cryopreservation. Adv Androl. 2014;2014: [9 p.].
 
Bussalleu E, Pinart E, Yeste M, Briz M, Sancho S, Garcia-Gil N, Badia E, Bassols J, Pruneda A, Casas I, Bonet S. Development of a protocol for multiple staining with fluorochromes to assess the functional status of boar spermatozoa. Microsc Res Tech. 2005 Dec 1;68(5):277-83. https://doi.org/10.1002/jemt.20246
 
Lv C, Larbi A, Wu G, Hong Q, Quan G. Improving the quality of cryopreserved goat semen with a commercial bull extender supplemented with resveratrol. Anim Reprod Sci. 2019 Sep;208:106-27.  https://doi.org/10.1016/j.anireprosci.2019.106127
 
Ly JD, Grubb DR, Lawen A. The mitochondrial membrane potential (Δψm) in apoptosis; An update. Apoptosis. 2003 Mar;8(2):115-28. https://doi.org/10.1023/A:1022945107762
 
Cerolini S, Zaniboni L, Maldjian A, Gliozzi T. Effect of docosahexaenoic acid and α-tocopherol enrichment in chicken sperm on semen quality, sperm lipid composition and susceptibility to peroxidation. Theriogenology. 2006 Sep 1;66(4):877-86.
 
Mandal R, Badyakar D, Chakrabarty J. Role of membrane lipid fatty acids in sperm cryopreservation. Adv Androl. 2014;2014: [9 p.]. https://doi.org/10.1155/2014/190542
 
Muino R, Fernandez M, Pena AI. Post-thaw survival and longevity of bull spermatozoa frozen with an egg yolk-based or two egg yolk-free extenders after an equilibration period of 18 h. Reprod Domest Anim. 2007 Jun;42(3):305-11.
 
Muino R, Fernandez M, Pena AI. Post-thaw survival and longevity of bull spermatozoa frozen with an egg yolk-based or two egg yolk-free extenders after an equilibration period of 18 h. Reprod Domest Anim. 2007 Jun;42(3):305-11. https://doi.org/10.1111/j.1439-0531.2006.00784.x
 
Quintero-Moreno A, Rigau T, Rodriguez-Gil JE. Regression analyses and motile sperm subpopulation structure study as improving tools in boar semen quality analysis. Theriogenology. 2004 Feb;61(4):673-90. https://doi.org/10.1016/S0093-691X(03)00248-6
 
Quintero-Moreno A, Rigau T, Rodriguez-Gil JE. Regression analyses and motile sperm subpopulation structure study as improving tools in boar semen quality analysis. Theriogenology. 2004 Feb;61(4):673-90. https://doi.org/10.1016/S0093-691X(03)00248-6
 
Chanapiwat P, Kaeoket K, Tummaruk P. Effects of DHA-enriched hen egg yolk and L-cysteine supplementation on quality of cryopreserved boar semen. Asian J Androl. 2009 Sep;11(5):600-8.
 
Rahal A, Kumar A, Singh V, Yadav B, Tiwari R, Chakraborty S, Dhama K. Oxidative stress, prooxidants, and antioxidants: The interplay. Biomed Res Int. 2014 Jan;2014:[19 p.]. https://doi.org/10.1155/2014/761264
 
Rahal A, Kumar A, Singh V, Yadav B, Tiwari R, Chakraborty S, Dhama K. Oxidative stress, prooxidants, and antioxidants: The interplay. Biomed Res Int. 2014 Jan;2014:[19 p.]. https://doi.org/10.1155/2014/761264
 
Do L, Namula Z, Luu V, Sato Y, Taniguchi M, Isobe T, Kikuchi K, Otoi T. Effect of sericin supplementation during in vitro maturation on the maturation, fertilization and development of porcine oocytes. Reprod Domest Anim. 2014 Apr;48(2):17-20. https://doi.org/10.1111/rda.12274
 
Ratchamak R, Vongpralub T, Boonkum W, Chankitisakul V. Cryopreservation and quality assessment of boar semen collected from bulk samples. Vet Med-Czech. 2019 May 28;64(5):209-16. https://doi.org/10.17221/125/2018-VETMED
 
Fair S, Doyle DN, Diskin MG, Hennessy AA, Kenny DA. The effect of dietary n-3 polyunsaturated fatty acids supplementation of rams on semen quality and subsequent quality of liquid stored semen. Theriogenology. 2014 Jan 15;81(2):210-9.  https://doi.org/10.1016/j.theriogenology.2013.09.002
 
Ratchamak R, Ratsiri T, Kheawkanha T, Vongpralub T, Boonkum W, Chankitisakul V. Evaluation of cryopreserved boar semen after supplementation sericin form silkworm (Bombyx mori) in semen extender. Anim Sci J. 2020 Jan-Dec;91(1):e13428.  https://doi.org/10.1111/asj.13428
 
Ratchamak R, Vongpralub T, Boonkum W, Chankitisakul V. Cryopreservation and quality assessment of boar semen collected from bulk samples. Vet Med-Czech. 2019 May 28;64(5):209-16.
 
Reddy VS, Yadav B, Yadav CL, Anand M, Swain DK, Kumar D, Kritania D, Madan AK, Kumar J, Yadav S. Effect of sericin supplementation on heat shock protein 70 (HSP70) expression, redox status and post thaw semen quality in goat. Cryobiology. 2018 Oct;84:33-9.
 
Isobe T, Ikebata Y, Onitsuka T, Wittayarat M, Sato Y, Taniguchi M, Otoi T. Effect of sericin on preimplantation development of bovine embryos cultured individually. Theriogenology. 2012 Sep 1;78(4):747-52.
 
Ratchamak R, Ratsiri T, Kheawkanha T, Vongpralub T, Boonkum W, Chankitisakul V. Evaluation of cryopreserved boar semen after supplementation sericin form silkworm (Bombyx mori) in semen extender. Anim Sci J. 2020 Jan-Dec;91(1):e13428.
 
Sasaki M, Kato Y, Yamada H, Terada S. Development of a novel serum-free freezing medium for mammalian cells using the silk protein sericin. Biotechnol Appl Biochem. 2005 Oct;42(2):183-8.
 
Tao W, Li M, Xie R. Preparation and structure of porous silk sericin materials. Macromol Mater Eng. 2005 Mar;290(3):188-94. https://doi.org/10.1002/mame.200400306
 
Reddy VS, Yadav B, Yadav CL, Anand M, Swain DK, Kumar D, Kritania D, Madan AK, Kumar J, Yadav S. Effect of sericin supplementation on heat shock protein 70 (HSP70) expression, redox status and post thaw semen quality in goat. Cryobiology. 2018 Oct;84:33-9.
 
Isobe T, Ikebata Y, Onitsuka T, Do LTK, Sato Y, Taniguchi M, Otoi T. Cryopreservation for bovine embryos in serum-free freezing medium containing silk protein sericin. Cryobiology. 2013 Oct;67(2):184-7.
 
Sasaki M, Kato Y, Yamada H, Terada S. Development of a novel serum-free freezing medium for mammalian cells using the silk protein sericin. Biotechnol Appl Biochem. 2005 Oct;42(2):183-8.  https://doi.org/10.1042/BA20050019
 
Ugur MR, Saber Abdelrahman A, Evans HC, Gilmore AA, Hitit M, Arifiantini RI, Purwantara B, Kaya A, Memili E. Advances in cryopreservation of bull sperm. Front Vet Sci. 2019 Aug 27;6: [15 p.].
 
Tao W, Li M, Xie R. Preparation and structure of porous silk sericin materials. Macromol Mater Eng. 2005 Mar;290(3):188-94. https://doi.org/10.1002/mame.200400306
 
Ugur MR, Saber Abdelrahman A, Evans HC, Gilmore AA, Hitit M, Arifiantini RI, Purwantara B, Kaya A, Memili E. Advances in cryopreservation of bull sperm. Front Vet Sci. 2019 Aug 27;6: [15 p.]. https://doi.org/10.3389/fvets.2019.00268
 
Yasmin C, Otoi T, Setiadi MA, Karja NWK. Maturation and fertilisation of sheep oocytes cultured in serum-free medium containing silk protein sericin. Acta Vet Hung. 2015 Mar 1;63(1):110-7.
 
Kato N, Sato S, Yamanaka A, Yamada H, Fuwa N, Nomura M. Silk protein, sericin, inhibits lipid peroxidation and tyrosinase activity. Biosci Biotechnol Biochem. 1998 Jan;62(1):145-7.
 
Kefer JC, Agarwal A, Sabanegh E. Role of antioxidants in the treatment of male infertility. Int J Urol. 2009;16(5):449-57.  https://doi.org/10.1111/j.1442-2042.2009.02280.x
 
Khan RU. Antioxidants and poultry semen quality. Worlds Poult Sci J. 2011 Jun;67(2):297-308.  https://doi.org/10.1017/S0043933911000316
 
Yasmin C, Otoi T, Setiadi MA, Karja NWK. Maturation and fertilisation of sheep oocytes cultured in serum-free medium containing silk protein sericin. Acta Vet Hung. 2015 Mar 1;63(1):110-7.
 
Kumar P, Kumar D, Sikka P, Singh P. Sericin supplementation improves semen freezability of buffalo bulls by minimizing oxidative stress during cryopreservation. Anim Reprod Sci. 2015 Jan;152:26-31.
 
Yeste M. Sperm cryopreservation update: Cryodamage, markers, and factors affecting the sperm freezability in pigs. Theriogenology. 2016 Jan 1;85(1):47-64.
 
Yeste M. Sperm cryopreservation update: Cryodamage, markers, and factors affecting the sperm freezability in pigs. Theriogenology. 2016 Jan 1;85(1):47-64.  https://doi.org/10.1016/j.theriogenology.2015.09.047
 
Lange-Consiglio A, Meucci A, Cremonesi F. Fluorescent multiple staining and CASA system to assess boar sperm viability and membranes integrity in short and long-term extenders. Open Vet J. 2013 Mar;3(1),21-35.
 
Wu JH, Wang Z, Xu SY. Preparation and characterization of sericin powder extracted from silk industry wastewater. Food Chem. 2007 Oct; 103(4): 1255–62.
 
Wu JH, Wang Z, Xu SY. Preparation and characterization of sericin powder extracted from silk industry wastewater. Food Chem. 2007 Oct; 103(4): 1255–62.
 
Losano JDA, Angrimani DSR, Rui BR, Bicudo LC, Dal-mazzo A, Silva BCS, Viana CHC, Mendes CM, Assumpcao MEOA, Barnabee VH, Nichi M. The addition of docosahexaenoic acid (DHA) and antioxidants (glutathione peroxidase and superoxide dismutase) in extenders to epididymal sperm cryopreservation in bulls. Zygote. 2018 Jun;26(3):199-206.
 
Lv C, Larbi A, Wu G, Hong Q, Quan G. Improving the quality of cryopreserved goat semen with a commercial bull extender supplemented with resveratrol. Anim Reprod Sci. 2019 Sep;208:106-27.  https://doi.org/10.1016/j.anireprosci.2019.106127
 
Ly JD, Grubb DR, Lawen A. The mitochondrial membrane potential (Δψm) in apoptosis; An update. Apoptosis. 2003 Mar;8(2):115-28. https://doi.org/10.1023/A:1022945107762
 
Mandal R, Badyakar D, Chakrabarty J. Role of membrane lipid fatty acids in sperm cryopreservation. Adv Androl. 2014;2014: [9 p.].
 
Muino R, Fernandez M, Pena AI. Post-thaw survival and longevity of bull spermatozoa frozen with an egg yolk-based or two egg yolk-free extenders after an equilibration period of 18 h. Reprod Domest Anim. 2007 Jun;42(3):305-11. https://doi.org/10.1111/j.1439-0531.2006.00784.x
 
Quintero-Moreno A, Rigau T, Rodriguez-Gil JE. Regression analyses and motile sperm subpopulation structure study as improving tools in boar semen quality analysis. Theriogenology. 2004 Feb;61(4):673-90. https://doi.org/10.1016/S0093-691X(03)00248-6
 
Rahal A, Kumar A, Singh V, Yadav B, Tiwari R, Chakraborty S, Dhama K. Oxidative stress, prooxidants, and antioxidants: The interplay. Biomed Res Int. 2014 Jan;2014:[19 p.]. https://doi.org/10.1155/2014/761264
 
Ratchamak R, Vongpralub T, Boonkum W, Chankitisakul V. Cryopreservation and quality assessment of boar semen collected from bulk samples. Vet Med-Czech. 2019 May 28;64(5):209-16.
 
Ratchamak R, Ratsiri T, Kheawkanha T, Vongpralub T, Boonkum W, Chankitisakul V. Evaluation of cryopreserved boar semen after supplementation sericin form silkworm (Bombyx mori) in semen extender. Anim Sci J. 2020 Jan-Dec;91(1):e13428.  https://doi.org/10.1111/asj.13428
 
Reddy VS, Yadav B, Yadav CL, Anand M, Swain DK, Kumar D, Kritania D, Madan AK, Kumar J, Yadav S. Effect of sericin supplementation on heat shock protein 70 (HSP70) expression, redox status and post thaw semen quality in goat. Cryobiology. 2018 Oct;84:33-9.  https://doi.org/10.1016/j.cryobiol.2018.08.005
 
Sasaki M, Kato Y, Yamada H, Terada S. Development of a novel serum-free freezing medium for mammalian cells using the silk protein sericin. Biotechnol Appl Biochem. 2005 Oct;42(2):183-8.  https://doi.org/10.1042/BA20050019
 
Tao W, Li M, Xie R. Preparation and structure of porous silk sericin materials. Macromol Mater Eng. 2005 Mar;290(3):188-94. https://doi.org/10.1002/mame.200400306
 
Ugur MR, Saber Abdelrahman A, Evans HC, Gilmore AA, Hitit M, Arifiantini RI, Purwantara B, Kaya A, Memili E. Advances in cryopreservation of bull sperm. Front Vet Sci. 2019 Aug 27;6: [15 p.]. https://doi.org/10.3389/fvets.2019.00268
 
Yasmin C, Otoi T, Setiadi MA, Karja NWK. Maturation and fertilisation of sheep oocytes cultured in serum-free medium containing silk protein sericin. Acta Vet Hung. 2015 Mar 1;63(1):110-7. https://doi.org/10.1556/avet.2015.009
 
Yeste M. Sperm cryopreservation update: Cryodamage, markers, and factors affecting the sperm freezability in pigs. Theriogenology. 2016 Jan 1;85(1):47-64.  https://doi.org/10.1016/j.theriogenology.2015.09.047
 
Wu JH, Wang Z, Xu SY. Preparation and characterization of sericin powder extracted from silk industry wastewater. Food Chem. 2007 Oct; 103(4): 1255–62.
 
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