Organic matter and crude protein digestibility predicted from nitrogen and fibre fractionation of festulolium hybrids

 

https://doi.org/10.17221/10/2018-CJASCitation:Homolka P., Koukolová M., Koukolová V. (2018): Organic matter and crude protein digestibility predicted from nitrogen and fibre fractionation of festulolium hybrids  . Czech J. Anim. Sci., 63: 272-279.
download PDF

The objectives of the experiment were to study nitrogen fractions of the Cornell Net Carbohydrate and Protein System (CNCPS) and digestibility of organic matter (OMD) and crude protein (CPD) along with the possibilities for predicting OMD and CPD based on chemical composition and CNCPS nitrogen fractions. Thirty samples of festulolium hybrids (fescue hybrids and ryegrass hybrids) commonly used and certified in the Czech Republic were analyzed for chemical composition, brutto energy, and nitrogen fractions, these fractions being A (non-protein nitrogen), B1 (rapidly degradable protein), B2 (intermediately degradable protein), B3 (slowly degradable protein), and C (unavailable protein). OMD and CPD were determined by near-infrared reflectance spectroscopy. Nitrogen fractions showed similar mean values for both analyzed groups of festulolium hybrids. Mean rapidly, intermediately, and slowly soluble fractions (B1, B2, and B3, respectively) were 29.2, 30.7, and 33.7 g/kg of dry matter (DM) for fescue hybrids and 21.7, 33.5, and 31.9 g/kg of DM for ryegrass hybrids. There was a trend for fraction B2 to decrease with advancing maturity in relation to neutral detergent insoluble protein value. The mean content of bound fraction C was 10.4 g/kg of DM for fescue hybrids and 7.3 g/kg of DM for ryegrass hybrids. Three groups of hybrids were evaluated statistically: fescue (I), ryegrass (II), and combined fescue/ryegrass (III). Better regression equations were found for fescue/ryegrass hybrids. High degrees of CPD determination were identified with predictors of individual nitrogen fractions (R2 = 0.975; P > 0.0001). OMD was described in regressions only using nitrogen fractions A and B1 (R2 = 0.500; P > 0.0001).

References:
Abbasi D., Rouzbehan Y., Rezaei J. (2012): Effect of harvest date and nitrogen fertilization rate on the nutritive value of amaranth forage (Amaranthus hypochondriacus). Animal Feed Science and Technology, 171, 6-13 https://doi.org/10.1016/j.anifeedsci.2011.09.014
 
Ahvenjärvi Seppo, Vanhatalo Aila, Huhtanen Pekka, Varvikko Tuomo (1999): Effects of Supplementation of a Grass Silage and Barley Diet with Urea, Rapeseed Meal and Heat-moisture-treated Rapeseed Cake on Omasal Digesta Flow and Milk Production in Lactating Dairy Cows. Acta Agriculturae Scandinavica, Section A - Animal Science, 49, 179-189 https://doi.org/10.1080/090647099424097
 
Alzueta C, Caballero R, Rebolé A, Treviño J, Gil A (2001): Crude protein fractions in common vetch (Vicia sativa L.) fresh forage during pod filling.. Journal of Animal Science, 79, 2449- https://doi.org/10.2527/2001.7992449x
 
AOAC (2005): Official Methods of Analysis of AOAC International. 18th Ed. Association of Official Analytical Chemists, Gaithersburg, USA.
 
Chrenková M., Čerešňáková Z., Weisbjerg M.R., Formelová Z., Poláčiková M., Vondráková M. (2014): Characterization of proteins in feeds according to the CNCPS and comparison to in situ parameters. Czech Journal of Animal Science, 59, 288-295 https://doi.org/10.17221/7499-CJAS
 
Ehsani P. (2007): Evaluation and comparing of qualitative and quantitative yield of four amaranth varieties in different harvesting date. MSc Thesis. Saveh, Iran: Islamic Azad University of Saveh.
 
Elghandour M.M.Y., Kholif A.E., Hernández J., Mariezcurrena M.D., López S., Camacho L.M., Márquez O., Salem A.Z.M. (2016): Influence of the addition of exogenous xylanase with or without pre-incubation on the in vitro ruminal fermentation of three fibrous feeds. Czech Journal of Animal Science, 61, 262-272 https://doi.org/10.17221/52/2015-CJAS
 
Fox D. G., Sniffen C. J., O'Connor J. D., Russell J. B., Van Soest P. J. (1992): A net carbohydrate and protein system for evaluating cattle diets: III. Cattle requirements and diet adequacy. Journal of Animal Science, 70, 3578-3596 https://doi.org/10.2527/1992.70113578x
 
Fox D.G, Tedeschi L.O, Tylutki T.P, Russell J.B, Van Amburgh M.E, Chase L.E, Pell A.N, Overton T.R (2004): The Cornell Net Carbohydrate and Protein System model for evaluating herd nutrition and nutrient excretion. Animal Feed Science and Technology, 112, 29-78 https://doi.org/10.1016/j.anifeedsci.2003.10.006
 
Harazim J., Pavelek L., Ceresnakova Z., Homolka P., Trinac-ty J., Jambor V., Pozdisek J., Zeman L. (1999): Determination of degradability of feed crude protein and amino acids in the rumen using the method in situ, nylon bag. In: Proc. Internat. Scientific Workshop Determination of the Use of Nutrients in Ruminants, Opava, Czech Republic, 115‒118.
 
Jančík F., Homolka P., Čermák B., Lád F. (2008): Determination of indigestible neutral detergent fibre contents of grasses and its prediction from chemical composition. Czech Journal of Animal Science, 53, 128-135 https://doi.org/10.17221/2716-CJAS
 
Kopecký D., Loureiro J., Zwierzykowski Z., Ghesquière M., Doležel J. (2006): Genome constitution and evolution in Lolium × Festuca hybrid cultivars (Festulolium). Theoretical and Applied Genetics, 113, 731-742 https://doi.org/10.1007/s00122-006-0341-z
 
Kopecky D., Lukaszewski A.J., Dolezel J. (2008): Cytogenetics of Festulolium (Festuca × Lolium hybrids). Cytogenetics and Plant Breeding, 120, 370–383.
 
Kubota Akito, Akiyama Yukio, Fujimori Masahiro, Kiyoshi Takako (2016): No decrease in f ratio (ratio of Festuca -specific genome region to the whole genome) in maternally derived progeny of festulolium ( Festuca pratensis × Lolium species) across generations. Grassland Science, 62, 55-60 https://doi.org/10.1111/grs.12103
 
Lanzas C., Broderick G.A., Fox D.G. (2008): Improved Feed Protein Fractionation Schemes for Formulating Rations with the Cornell Net Carbohydrate and Protein System. Journal of Dairy Science, 91, 4881-4891 https://doi.org/10.3168/jds.2008-1440
 
Licitra G., Hernandez T.M., Van Soest P.J. (1996): Standardization of procedures for nitrogen fractionation of ruminant feeds. Animal Feed Science and Technology, 57, 347-358 https://doi.org/10.1016/0377-8401(95)00837-3
 
Liu D., Wu P., Jiao P. (2016): Researching rumen degradation behaviour of protein by FTIR spectroscopy. Czech Journal of Animal Science, 60, 25-32 https://doi.org/10.17221/7908-CJAS
 
Liu D., Li Y., Zhang G., Zhang P., Wu P., Wang S., Wang X. (2017): Effect of protein secondary structures in mixed feedstuff detected by Fourier transform infrared spectroscopy on ruminal protein degradation kinetics. Czech Journal of Animal Science, 62, 89-97 https://doi.org/10.17221/94/2015-CJAS
 
Loučka R., Tyrolová Y., Jančík F., Kubelková P., Homolka P., Jambor V. (2017): Variation for in vivo digestibility in two maize hybrid silages. Czech Journal of Animal Science, 63, 17-23 https://doi.org/10.17221/37/2017-CJAS
 
Pichard G., Van Soest P.J. (1977): Protein solubility of ruminant feeds. In: Proc. Cornell Nutrition Conference for Feed Manufacturers, Ithaca, USA, 91–98.
 
Purwin C., Pysera B., Fijalkowska M., Antoszkiewicz Z., Piwczynski D., Wyzlic I., Lipinski K. (2012): The influence of ensiling method on the composition of nitrogen fractions in red clover, alfalfa and red fescue silage. In: Proc. XVI Internat. Silage Conference, Hameenlinna, Finland, 256–257.
 
Schwab C.G., Tylutki T.P., Ordway R.S., Sheaffer C., Stern M.D. (2003): Characterization of Proteins in Feeds. Journal of Dairy Science, 86, E88-E103 https://doi.org/10.3168/jds.S0022-0302(03)74042-9
 
Valdés Carmen, Andrés Sonia, Giráldez F Javier, García Ricardo, Calleja Alfredo (2006): Potential use of visible and near infrared reflectance spectroscopy for the estimation of nitrogen fractions in forages harvested from permanent meadows. Journal of the Science of Food and Agriculture, 86, 308-314 https://doi.org/10.1002/jsfa.2309
 
Valiente O.L., Andueza D., de Vega A., Olmos G., Munoz F. (2004): The use of NIRS for prediction of intake, digestibility and diet composition in sheep fed mixed grain: roughage diets. Journal of Animal and Feed Sciences, 13, 227−230.
 
Van Soest P.J., Robertson J.B., Lewis B.A. (1991): Methods for Dietary Fiber, Neutral Detergent Fiber, and Nonstarch Polysaccharides in Relation to Animal Nutrition. Journal of Dairy Science, 74, 3583-3597 https://doi.org/10.3168/jds.S0022-0302(91)78551-2
 
Villamide M.J., Fraga M.J. (1998): Prediction of the digestible crude protein and protein digestibility of feed ingredients for rabbits from chemical analysis. Animal Feed Science and Technology, 70, 211-224 https://doi.org/10.1016/S0377-8401(97)00079-5
 
Wang S.P., Wang W.J., Yang D.S., Zhao X.L., Luo D.M., Guo Y.B. (2017): Growth, carcass, and physiological traits of growing male China Micro-ducks fed various levels of dietary crude fibre. Czech Journal of Animal Science, 62, 347-356 https://doi.org/10.17221/5/2017-CJAS
 
Xia Ning, Wang Jin-Mei, Gong Qian, Yang Xiao-Quan, Yin Shou-Wei, Qi Jun-Ru (2012): Characterization and In Vitro digestibility of rice protein prepared by enzyme-assisted microfluidization: Comparison to alkaline extraction. Journal of Cereal Science, 56, 482-489 https://doi.org/10.1016/j.jcs.2012.06.008
 
Yamada Toshihiko, Forster John W., Humphreys Michael W., Takamizo Tadashi (2005): REVIEW. Genetics and molecular breeding in Lolium/Festuca grass species complex. Grassland Science, 51, 89-106 https://doi.org/10.1111/j.1744-697X.2005.00024.x
 
download PDF

© 2019 Czech Academy of Agricultural Sciences