Effect of thawing method on bull sperm survival in ejaculates frozen in 4 ml and 8 ml volumes


https://doi.org/10.17221/117/2018-CJASCitation:Biniová Z., Stádník L., Doležalová M., Ducháček J. (2018): Effect of thawing method on bull sperm survival in ejaculates frozen in 4 ml and 8 ml volumes  . Czech J. Anim. Sci., 63: 399-407.
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The aim of this study was to evaluate the effect of different thawing protocols (slow (P1), medium (P2), and fast (P3)) on percentage of motile sperm (MOT) and percentage of sperm cells with intact membranes (INT) in Holstein (4 bulls; 72 samples) and Czech Fleckvieh (4 bulls; 72 samples) semen frozen-thawed in 4 ml and 8 ml volumes. MOT was analysed in fresh semen, as well as immediately after thawing (T0) and 30 min after thawing (T30). INT was analysed using hypoosmotic swelling test (HOS test) in fresh ejaculate (FE), after diluting (DE), and at T0. The differences between FE parameters and frozen-thawed ejaculate parameters, expressing changes that occur during cryopreservation, were calculated. Apart from the effect of thawing protocol, the effect of breed and the effect of quality of FE expressed by MOT immediately after collecting were evaluated, too. Unlike thawing of semen cryopreserved in straws (0.25 and 0.5 ml), thawing using the slow protocol (P1) was the most appropriate (P < 0.05) for both observed volumes. There were found significantly higher MOT in the volume of 8 ml in both T0 and T30 and in the volume of 4 ml in T30 in samples thawed using P1 and P2. MOT in T0 was significantly affected by breed in samples frozen in 8 ml and in T30 in samples frozen in 4 and 8 ml. There were found no significant differences in INT in all reported volumes, however decrease of INT during cryopreservation was affected by breed.

Arav Amir, Yavin Saar, Zeron Yoel, Natan Dity, Dekel Izik, Gacitua Haim (2002): New trends in gamete's cryopreservation. Molecular and Cellular Endocrinology, 187, 77-81  https://doi.org/10.1016/S0303-7207(01)00700-6
Barth A.D., Bowman B., Bowman P.A. (1988): Determination of the best practical method of thawing bovine semen. Canadian Veterinary Journal, 29, 366–369.
Beran Jan, Stádník Luděk, Ducháček Jaromír, Toušová Renáta, Louda František, Štolc Ladislav (2011): Effect of bulls' breed, age and body condition score on quantitative and qualitative traits of their semen. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 59, 37-44  https://doi.org/10.11118/actaun201159060037
Beran J., Stadnik L., Bezdicek J., Louda F., Citek J., Duchacek J. (2012): Effect of sire and extender on sperm motility and share of live or dead sperm in bulls’ fresh ejaculate and in AI doses after thawing. Archiv für Tierzucht, 55, 207–218.
Brannigan R., Lipshultz L. (2008): Sperm transport and capacitation. The Global Library of Women’s Medicine. Available at http://editorial.glowm.com/?p=glowm.cml/section_view&articleid=315 (accessed May 13, 2017).
Brinsko S.P., Van Wagner G.S., Graham J.K., Squires E.L. (2000): Motility, morphology and triple stain analysis of fresh, cooled and frozen-thawed stallion spermatozoa. Journal of Reproduction and Fertility, 56, 111–120.
Doležalová M., Stádník L., Biniová Z., Ducháček J., Stupka R. (2016): Equilibration and freezing interactions affecting bull sperm characteristics after thawing. Czech Journal of Animal Science, 61, 515-525  https://doi.org/10.17221/23/2016-CJAS
Gacitua H., Arav A. (2005): Successful pregnancies with directional freezing of large volume buck semen. Theriogenology, 63, 931-938  https://doi.org/10.1016/j.theriogenology.2004.05.012
Gloria A., Carluccio A., Contri A., Wegher L., Valorz C., Robbe D. (2013): The effect of the chamber on kinetic results in cryopreserved bull spermatozoa. Andrology, 1, 879-885  https://doi.org/10.1111/j.2047-2927.2013.00121.x
Holt W.V., Del Valle I., Fazeli A. (2015): Heat shock protein A8 stabilizes the bull sperm plasma membrane during cryopreservation: Effects of breed, protein concentration, and mode of use. Theriogenology, 84, 693-701  https://doi.org/10.1016/j.theriogenology.2015.05.004
Horváth Ákos, Miskolczi Edit, Mihálffy Szilvia, Ősz Katalin, Szabó Krisztián, Urbányi Béla (2007): Cryopreservation of common carp (Cyprinus carpio) sperm in 1.2 and 5ml straws and occurrence of haploids among larvae produced with cryopreserved sperm. Cryobiology, 54, 251-257  https://doi.org/10.1016/j.cryobiol.2007.02.003
James P.S., Wolfe C.A., Mackie A., Ladha S., Prentice A., Jones R. (1999): Lipid dynamics in the plasma membrane of fresh and cryopreserved human spermatozoa. Human Reproduction, 14, 1827-1832  https://doi.org/10.1093/humrep/14.7.1827
Jeyendran R. S., Van der Ven H. H., Perez-Pelaez M., Crabo B. G., Zaneveld L. J. D. (1984): Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics. Reproduction, 70, 219-228  https://doi.org/10.1530/jrf.0.0700219
Kohsaka T., Takahara H., Tagami S., Sasada H., Masaki J. (1992): A new technique for the precise location of lactate and malate dehydrogenases in goat, boar and water buffalo spermatozoa using gel incubation film. Reproduction, 95, 201-209  https://doi.org/10.1530/jrf.0.0950201
Lepock J. R. (1993): Protein denaturation in intact hepatocytes and isolated cellular organelles during heat shock. The Journal of Cell Biology, 122, 1267-1276  https://doi.org/10.1083/jcb.122.6.1267
Li Y., Sun M., Zhu J., Jiao G., Lin J., Dong H., Tan J., Zhou J. (2017): Species difference in the expression of Fas and Fas ligand in mature mammalian spermatozoa. Czech Journal of Animal Science, 62, 519-526  https://doi.org/10.17221/18/2017-CJAS
Maxwell WMC, Parrilla I, Caballero I, Garcia E, Roca J, Martinez EA, Vazquez JM, Rath D (2007): Retained Functional Integrity of Bull Spermatozoa after Double Freezing and Thawing Using PureSperm® Density Gradient Centrifugation. Reproduction in Domestic Animals, 42, 489-494  https://doi.org/10.1111/j.1439-0531.2006.00811.x
Mortimer S.T. (2000): CASA – practical aspects. Journal of Andrology, 21, 515–524.
Padrik P., Hallap T., Kaart T., Bulitko T., Jaakma Ü. (2012): &nbsp;Relationships between the results of hypo-osmotic swelling tests, sperm motility, and fertility in Estonian Holstein dairy bulls. Czech Journal of Animal Science, 57, 490-497  https://doi.org/10.17221/6349-CJAS
Pons-Rejraji H., Bailey J.L., Leclerc P. (2009): Cryopreservation affects bovine sperm intracellular parameters associated with capacitation and acrosome exocytosis. Reproduction, Fertility and Development, 21, 525−537.
Ramu S., Jeyendran R.S. (2013): Thy hypo-osmotic swelling test for evaluation of sperm membrane integrity. Methods of Molecular Biology, 927, 21–25.
Rastegarnia A., Shahverdi A., Rezaei T.T., Ebrahimi B., Shafipour V. (2013): Effect of different thawing rates on post-thaw viability, kinematic parameters and chromatin structure of buffalo (Bubalus bubalis) spermatozoa. Cell Journal, 14, 306–313.
Saragusty J, Gacitua H, Pettit MT, Arav A (2007): Directional Freezing of Equine Semen in Large Volumes. Reproduction in Domestic Animals, 42, 610-615  https://doi.org/10.1111/j.1439-0531.2006.00831.x
Saragusty Joseph, Gacitua Haim, Zeron Yoel, Rozenboim Israel, Arav Amir (2009): Double freezing of bovine semen. Animal Reproduction Science, 115, 10-17  https://doi.org/10.1016/j.anireprosci.2008.11.005
Si Wei, Hildebrandt Thomas B., Reid Catherine, Krieg Ronald, Ji Weizhi, Fassbender Mirja, Hermes Robert (2006): The successful double cryopreservation of rabbit (Oryctolagus cuniculus) semen in large volume using the directional freezing technique with reduced concentration of cryoprotectant. Theriogenology, 65, 788-798  https://doi.org/10.1016/j.theriogenology.2005.06.010
Sichtar J., Nehasilova A., Simonik O., Bubenickova F. (2017): Effect of two freezing extenders on characteristic of fresh and frozen-thawed semen in endangered Old Kladruber stallions – a pilot study. Czech Journal of Animal Science, 62, 227−233.
Simonik O., Sichtar J., Krejcarkova A., Rajmon R., Stadnik L., Beran J., Dolezalova M., Biniova Z. (2015): Computer Assisted Sperm Analysis – the relationship to bull field fertility, possible errors and their impact on outputs: A review. Indian Journal of Animal Sciences, 85, 3–11.
Slanina T., Miškeje M., Tirpák F., Błaszczyk M., Stawarz R., Massányi P. (2018): Effect of taurine on turkey (Meleagris gallopavo) spermatozoa viability and motility. Czech Journal of Animal Science, 63, 127-135  https://doi.org/10.17221/79/2017-CJAS
Stádník Luděk, Rajmon Radko, Beran Jan, Šimoník Ondřej, Doležalová Martina, Šichtař Jiří, Stupka Roman, Folková Petra (2015): Influence of selected factors on bovine spermatozoa cold shock resistance. Acta Veterinaria Brno, 84, 125-131  https://doi.org/10.2754/avb201584020125
Taşdemir Umut, Büyükleblebici Serhat, Tuncer Pürhan Barbaros, Coşkun Erdem, Özgürtaş Taner, Aydın Fevzi Nuri, Büyükleblebici Olga, Gürcan İsmail Safa (2013): Effects of various cryoprotectants on bull sperm quality, DNA integrity and oxidative stress parameters. Cryobiology, 66, 38-42  https://doi.org/10.1016/j.cryobiol.2012.10.006
Thurston L.M., Watson P.F., Holt W.V. (2002): Semen cryopreservation: A genetic explanation for species and individual variation? Cryo Letters, 23, 255–262.
Tvrdá E., Greifová H., Mackovich A., Hashim F., Lukáč N. (2018): Curcumin offers antioxidant protection to cryopreserved bovine semen. Czech Journal of Animal Science, 63, 247-255  https://doi.org/10.17221/33/2017-CJAS
Underwood S.L., Bathgate R., Maxwell W.M.C., Evans G. (2009): In vitro characteristics of frozen-thawed, sex-sorted bull sperm after refreezing or incubation at 15 or 37 °C. Theriogenology, 72, 1001-1008  https://doi.org/10.1016/j.theriogenology.2009.06.023
Underwood S.L., Bathgate R., Maxwell W.M.C., Evans G. (2010): Birth of offspring after artificial insemination of heifers with frozen-thawed, sex-sorted, re-frozen-thawed bull sperm. Animal Reproduction Science, 118, 171-175  https://doi.org/10.1016/j.anireprosci.2009.08.007
Verstegen J., Iguer-Ouada M., Onclin K. (2002): Computer assisted semen analyzers in andrology research and veterinary practice. Theriogenology, 57, 149-179  https://doi.org/10.1016/S0093-691X(01)00664-1
Watson P.F (2000): The causes of reduced fertility with cryopreserved semen. Animal Reproduction Science, 60-61, 481-492  https://doi.org/10.1016/S0378-4320(00)00099-3
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