Effects ofthree kinds ofprotease ongrowth performance, apparent digestibility ofnutrients and caecal microbial counts inweanling pigs


Ma W., Lv Y., Guo L., Wang Z., Zhao F. (2020): Effects of three kinds of protease on growth performance, apparent digestibility of nutrients and caecal microbial counts in weanling pigs. Czech J. Anim. Sci., 65: 373–379.

download PDF

This experiment investigated the effects of three single kinds of protease on performance, serum parameters, apparent digestibility of nutrients and caecal microorganisms in weanling pigs. One hundred and ninety-two White × Landrace × Duroc hybrid pigs (7.51 ± 0.81 kg) were allotted to 1 of 4 treatments for 28 days. No protease was added to the control diet and the three experimental diets were supplemented with the same proportion of 10 IU/kg of alkaline, acidic and neutral protease, respectively. The supplementation of alkaline protease increased average daily gain and reduced serum concentrations of cholesterol and triglyceride compared with the control group (P < 0.05). The three single kinds of protease supplementation decreased the levels of serum urea nitrogen and increased the serum concentration of aspartate aminotransferase (P < 0.05). The apparent digestibility of crude protein was increased by the dietary supplementation of alkaline or acidic protease compared with that of pigs in the control group (P < 0.05). Results indicate that the alkaline protease supplementation improved the performance and apparent digestibility of crude protein in weanling pigs.

Amerah AM, Romero LF, Awati A, Ravindran V. Effect of exogenous xylanase, amylase, and protease as single or combined activities on nutrient digestibility and growth performance of broilers fed corn/soy diets. Poultry Sci. 2017 Apr 1;96(4):807-16. https://doi.org/10.3382/ps/pew297
Anwar A, Saleemuddin M. Alkaline proteases: A review. Bioresource Technol. 1998 Jun;64(3):175-83. https://doi.org/10.1016/S0960-8524(97)00182-X
AOAC – Association of Official Analytical Chemists. Official methods of analysis. 17th ed. Arlington, VA: Association of Official Analytical Chemists; 2003.
Barekatain MR, Antipatis C, Choct M, Iji PA. Interaction between protease and xylanase in broiler chicken diets containing sorghum distillers’ dried grains with solubles. Anim Feed Sci Tech. 2013 Jun;182(1-4):71-81.
Campbell GL, Bedford MR. Enzyme applications for monogastric feeds: A review. Can J Anim Sci. 1992 Sep;72(3):449-66. https://doi.org/10.4141/cjas92-058
Chen H, Zhang S, Park I, Kim SW. Impacts of energy feeds and supplemental protease on growth performance, nutrient digestibility, and gut health of pigs from 18 to 45 kg body weight. Anim Nutr. 2017 Dec;3(4):359-65.  https://doi.org/10.1016/j.aninu.2017.09.005
Cowieson AJ, Roos FF. Toward optimal value creation through the application of exogenous mono-component protease in the diets of non-ruminants. Anim Feed Sci Tech. 2016 Sep;221:331-40.
Cowieson AJ, Zaefarian F, Knap I, Ravindran V. Interactive effects of dietary protein concentration, a mono-component exogenous protease and ascorbic acid on broiler performance, nutritional status and gut health. Anim Prod Sci. 2017 July;57(6):1058-68.
Guggenbuhl P, Wache Y, Wilson JW. Effects of dietary supplementation with a protease on the apparent ileal di-gestibility of the weaned piglet. J Anim Sci. 2012;90:152-4. https://doi.org/10.2527/jas.53835
Gurumallesh P, Alagu K, Ramakrishnan B, Muthusamy S. A systematic reconsideration on proteases. Int J Biol. Macromol. 2019 May 1;128:254-67. https://doi.org/10.1016/j.ijbiomac.2019.01.081
Kiarie E, Romero LF, Nyachoti CM. The role of added feed enzymes in promoting gut health in swine and poultry. Nutr Res Rev. 2013 Jun;26(1):71-88. https://doi.org/10.1017/S0954422413000048
Kiarie E, Walsh MC, Nyachoti CM. Performance, digestive function, and mucosal responses to selected feed additives for pigs. J Anim Sci. 2016 Sep;94(Suppl_3):169-80.  https://doi.org/10.2527/jas.2015-9835
Kim JC, Hansen CF, Mullan BP, Pluske JR. Nutrition and pathology of weaner pigs: Nutritional strategies to support barrier function in the gastrointestinal tract. Anim Feed Sci Tech. 2012 Apr;173(1-2):3-16.
Ma WF, Mao P, Guo L, Qiao SY. Crystalline amino acids supplementation improves the performance and carcass traits in late-finishing gilts fed low-protein diets. Anim Sci J. 2020 Jan;91(1):e13317. https://doi.org/10.1111/asj.13317
Norgaard JV, Malla N, Dionisio G, Madsen CK, Pettersson D, Laerke HN, Hjortshoj RL, Brinch-Pedersen, H. Exogenous xylanase or protease for pigs fed barley cultivars with high or low enzyme inhibitors. Anim Feed Sci Tech. 2019 Feb;248:59-66.
NRC – National Research Council. Nutrients requirements of swine. 11th ed. Washington DC., USA: National Academies Press; 2012.
O’Shea CJ, Mc Alpine PO, Solan P, Curran T, Varley PF, Walsh AM, Doherty JVO. The effect of protease and xylanase enzymes on growth performance, nutrient digestibility, and manure odour in grower-finisher pigs. Anim Feed Sci Tech. 2014 Mar;189:88-97. https://doi.org/10.1016/j.anifeedsci.2013.11.012
Pan L, Zhao PF, Yang ZY, Long SF, Wang HL, Tian QY, Xu YT, Xu X, Zhang ZH, Piao XS. Effects of coated compound proteases on apparent total tract digestibility of nutrients and apparent ileal digestibility of amino acids for pigs. Asian Austral J Anim Sci. 2016 Dec;29(12):1761-7.
Sauer N, Eklund M, Bauer E, Ganzle MG, Field CJ, Zijlstra RT, Mosenthin R. The effects of pure nucleotides on performance, humoral immunity, gut structure and numbers of intestinal bacteria of newly weaned pigs. J Anim Sci. 2012 Sep;90(9):3126-34.
Sun P, Li D, Dong B, Qiao S, Ma X. Effects of soybean glycinin on performance and immune function in early weaned pigs. Arch Anim Nutr. 2008 Jul;62(4):313-21.
Torres-Pitarch A, Hermans D, Manzanilla EG, Bindell J, Everaert N, Beckers Y, Lawlor PG. Effect of feed enzymes on digestibility and growth in weaned pigs: A systematic review and meta-analysis. Anim Feed Sci Tech. 2017 Nov;233:145-59. https://doi.org/10.1016/j.anifeedsci.2017.04.024
Valencia Z, Chavez ER. Phytase and acetic acid supplementation in the diet of early weaned piglets: Effect on performance and apparent nutrient digestibility. Nutr Res. 2002 May;22(5):623-32.
Zhang LY, Li D, Qiao S, Johnson EW, Li B, Thacker PA, Han IK. Effects of stachyose on performance, diarrhoea incidence and intestinal bacteria in weanling pigs. Arch Anim Nutr. 2003 Feb;57(1):1-10.
Zuo J, Ling B, Long L, Li T, Lahaye L, Yang C, Feng D. Effect of dietary supplementation with protease on growth performance, nutrient digestibility, intestinal morphology, digestive enzymes and gene expression of weaned piglets. Anim Nutr. 2015 Dec;1(4):276-82. https://doi.org/10.1016/j.aninu.2015.10.003
download PDF

© 2020 Czech Academy of Agricultural Sciences | Prohlášení o přístupnosti