Transformation of a short-term boar semen extender into a long-term boar semen extender by using penicillamine

Chanapiwat P., Buranasinsup S., Kaeoket K. (2022): Transformation of a short-term boar semen extender into a long-term boar semen extender by using penicillamine. Czech J. Anim. Sci., 67: 407–415.

download PDF

This study aimed to convert a short-term semen extender into a long-term semen extender by adding penicillamine (PEN). Semen samples were collected from six boars, diluted at a concentration 3 × 109 spermatozoa/100 ml, and divided into eight groups as follows: BTS, Merck III®, Androstar® Plus, PEN 0.125 mM, PEN 0.25 mM, PEN 0.5 mM, PEN 1.0 mM, PEN 2.0 mM. All the diluted semen samples were stored at 18 °C and were examined for progressive motility, viability, acrosome integrity, pH and osmolarity on days 0, 1, 3, 5 and 7 after storage. The level of the total anti-oxidative capability (T-AOC), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and malondialdehyde (MDA) were also measured for antioxidant activities. Fertility tests on a commercial pig farm was also conducted. On days 0, 1 and 3 of storage, no statistically significant differences in all the parameters among the extenders was observed. On day 5, the semen diluted with PEN at a concentration of 0.25 mM and Androstar® Plus could maintain progressive motility reach of 70.0% and 76.0%, respectively (P < 0.01). On day 7, the semen diluted with PEN at a concentration of 0.25 mM maintained progressive motility (70.0%) and acrosome integrity (85.7%) which is comparable to long-term semen extenders (Androstar® Plus) (P < 0.01). For the antioxidant activities, the level of the T-AOC, SOD and MDA varied depended on the PEN concentrations . The results of the fertility test were not different among the groups. To conclude, adding PEN at 0.25 mM in the BTS extender is able to transform a short-term semen extender into a long-term extender.

Aitken RJ, Curry BJ. Redox regulation of human sperm function: From the physiological control of sperm capacitation to the etiology of infertility and DNA damage in the germ line. Antioxid Redox Signal. 2011 Feb;14(3):367-81.
Aitken RJ, Gibb Z, Mitchell LA, Lambourne SR, Connaughton HS, De Iuliis GN. Sperm motility is lost in vitro as a consequence of mitochondrial free radical production and the generation of electrophilic aldehydes but can be significantly rescued by the presence of nucleophilic thiols. Biol Reprod. 2012 Nov 1;87(5):1-11.
Alvarez JG, Storey BT. Differential incorporation of fatty acids into and peroxidative loss of fatty acids from phospholipids of human spermatozoa. Mol Reprod Dev. 1995 Nov;42(3):334-46.
Bluestone R, Grahame R, Holloway V, Holt PJL. Treatment of systemic sclerosis with D-penicillamine. Ann Rheum Dis. 1970 Mar;29(2):153-8.
Chanapiwat P, Kaeoket K. Cryopreservation of boar semen: Where we are. Thai J Vet Med. 2020 Sep;50(3):283-95.
Chanapiwat P, Kaeoket K. L-cysteine prolonged fresh boar semen qualities, but not for docosahexaenoic acid (DHA). Czech J Anim Sci. 2021 Jan 25;66(1):21-8.
Chanapiwat P, Kaeoket K, Tummaruk P. Effects of DHA-enriched hen egg yolk and L-cysteine supplementation on quality of cryopreserved boar semen. Asian J Androl. 2009 Sep 1;11(5):600-8.
De Lamirande E, Jiang H, Zini A, Kodama H, Gagnon C. Reactive oxygen species and sperm physiology. Rev Reprod. 1997 Jan 1;2:48-54.
Diezel W, Engel S, Sonnichsen N, Hohne WE. Lipid peroxidation products in human spermatozoa: Detection and pathogenic significance. Andrologia. 1980 Mar-Apr;12(2):167-71.
El-Shahat KH, Taysser MI, Badr MR, Zaki KA. Effects of penicillamine, hypotaurine, and epinephrine on motility, hyperactivity, acrosome reaction of fresh ram sperm. Asian Pac J Reprod. 2017 Nov 1;6(6):283-8.
Funahashi H, Sano T. Select antioxidants improve the function of extended boar semen stored at 10 °C. Theriogenology. 2005 Apr 1;63(6):1605-16.
Gadea J. Semen extenders used in the artificial insemination of swine, A review. Spanish J Agri Res. 2003 Jun;1(2):17-27.
Guthrie HD, Liu J, Critser JK. Osmotic tolerance limits and effects of cryoprotectants on motility of bovine spermatozoa. Biol Reprod. 2002 Dec 1;67(6):1811-6.
Hu J, Geng G, Li Q, Sun X, Cao H, Liu Y. Effects of alginate on frozen-thawed boar spermatozoa quality, lipid peroxidation and antioxidant enzymes activities. Anim Reprod Sci. 2014 Jun 30;147(3-4):112-8.
Jones R, Mann T, Sherins RJ. Peroxidative breakdown of phospholipids in human spermatozoa: Spermicidal effects of fatty acid peroxides and protective action of seminal plasma. Fertil Steril. 1979 May;31(5):531-7.
Junker P. D-penicillamine in rheumatoid arthritis. Indian J Pediatr. 1986 Sep-Oct;53(5):625-8.
Kaeoket K, Persson E, Dalin AM. The influence of pre- and post-ovulatory insemination on sperm distribution in the oviduct, accessory sperm to the zona pellucida, fertilisation rate and embryo development in sows. Anim Reprod Sci. 2002 Jun;71(3-4):239-48.
Kaeoket K, Persson E, Dalin AM. Influence of pre-ovulatory insemination and early pregnancy on the distribution of CD2, CD4, CD8 and MHC class II expressing cells in the sow endometrium. Anim Reprod Sci. 2003 Apr;76(3-4):231-44.
Kaeoket K, Tantasuparuk W, Kunavongkrit A. The effect of post-ovulatory insemination on the subsequent embryonic loss, oestrous cycle length and vaginal discharge in sows. Reprod Domest Anim. 2005 Oct;40(5):492-4.
Kaeoket K, Srisowanna T, Wichaidit U, Chanapiwat P, Manee-in S. Comparative study on six different long term commercial extenders for fresh boar semen. Thai J Vet Med. 2010a Sep;40(3):257-63.
Kaeoket K, Sang-urai P, Thamniyom A, Chanapiwat P, Techakumphu M. Effect of docosahexaenoic acid (DHA) on quality of cryopreserved boar semen in different breeds. Reprod Domest Anim. 2010b Jun;45(3):458-63.
Kaeoket K, Chanapiwat P, Tummaruk P, Techakumphu M. Supplemental effect of varying L-cysteine concentrations on the quality of cryopreserved boar semen. Asian J Androl. 2010c Sep;12(5):760-5.
Kaeoket K, Donto S, Nualnoy P, Noiphinit J, Chanapiwat P. Effect of gamma-oryzanol-enriched rice bran oil on quality of cryopreserved boar semen. J Vet Med Sci. 2012 Sep;74(9):1149-53.
Kazemi K, Geramizadeh B, Nikeghbalian S, Salahi H, Bahador A, Nejatollahi SMR, Dehghani SM, Dehghani M, Kakaei F, Malek-Hosseini SA. Effect of D-penicillamine on liver fibrosis and inflammation in Wilson disease. Exp Clin Transplant. 2008 Dec;6(4):261-3.
Knox R, Levis D, Safranski T, Singleton W. An update on North American boar stud practices. Theriogenology. 2008 Nov;70(8):1202-8.
Kumaresan A, Kadirvel G, Bujarbaruah KM, Bardoloi RK, Anubrata D, Kumar S, Naskar S. Preservation of boar semen at 18 °C induces lipid peroxidation and apoptosis like changes in spermatozoa. Anim Reprod Sci. 2009 Jan;110(1-2):162-71.
Mapeka MH, Lehloenya KC, Nedambale TL. Comparison of different extenders and storage temperature on the sperm motility characteristics of Kolbroek pig semen. S Afr J Anim Sci. 2012 Aug;42(5):530-4.
Person CN, Lester TD, Person MD, Rorie RW. Effects of penicillamine, hypotaurine and epinephrine (PHE) on post-thaw bovine sperm parameters, as measured by computer-assisted sperm analysis. Ark Agr Exp Stat Res. 2007;553:47-50.
Sikka SC, Rajasekaran M, Hellstrom WJG. Role of oxidative stress and antioxidants in male infertility. J Androl. 1995 Nov-Dec;16(6):464-8.
Wang P, Li BL, Li NB, Luo HQ. A fluorescence detection of D-penicillamine based on Cu2+ induced fluorescence quenching system of protein-stabilized gold nanoclusters. Spectrochim Acta A Mol Biomol Spectrosc. 2015 Jan 25;135:198-202.
download PDF

© 2022 Czech Academy of Agricultural Sciences | Prohlášení o přístupnosti