Effect of protease supplementation on the digestibility of amino acids in animal-origin meals for broiler diets

https://doi.org/10.17221/134/2020-CJASCitation:

da Silva J.M.S., de Oliveira N.R., Vieira R.A., dos Santos R.O.F., Minafra C.S., dos Santos F.R. (2021): Effect of protease supplementation on the digestibility of amino acids in animal-origin meals for broiler diets. Czech J. Anim. Sci., 66: 29–37

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Enzymes benefit digestion and absorption of the ingredients and their addition to an animal-origin meal (AOM) can improve its nutritional quality. This research aimed to evaluate the effect of protease on nutrient digestibility, amino acids, and metabolism of AOM energy for broilers. Four hundred and eighty broiler chickens were distributed in a completely randomized design (4 × 2 factorial scheme), eight treatments, six replicates containing 10 birds/replicate. Treatments consisted of poultry viscera meal, swine viscera meal (SVM), bovine meat and bone meal, and basal diet; with and without protease addition. Two tests were performed. In the first test, the total excreta collection method was used with birds at 13 to 20 days of age and 25% of the reference feed was replaced by AOM. In the second test, a protein-free diet was administered to birds at 21 to 24 days of age and AOM replaced 25% of the starch. The inclusion of protease increased the apparent metabolizable energy corrected for nitrogen balance of SVM by 15.99% and the apparent metabolizable crude energy by 5.7%, and it also raised the coefficient of true ileal digestibility of the amino acids in the AOMs by 5.67% on average. The inclusion of protease improved the apparent metabolizable crude energy of AOMs, apparent metabolizable dry matter of bovine meat and bone meal, coefficient of true ileal digestibility of essential amino acids, and apparent metabolizable energy corrected for nitrogen balance of SVM. Dietary supplementation of protease may be a potential strategy to improve the digestibility of amino acids for broilers, a possibility of using animal-origin meals as a protein source of diets.

References:
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Matias CFQ, Rocha JSR, Pompeu MA, Baiao RC, Baiao NC, Lara LJC, Cardeal PC. Effect of protease on the metabolization coefficient of nutrients in broilers. Arq Bras Med Vet Zootec. 2015 Apr;67(2):492-8. https://doi.org/10.1590/1678-7883
 
Matterson LD, Potter LM, Stutz MW, Singsen EP. The metabolizable energy of feed ingredients for chickens. Res Rep Conn Agric Exp Stn. 1965 Mar;7(1):11-4.
 
Olukosi OA, Beeson LA, Englyst K, Romero LF. Effects of exogenous proteases without or with carbohydrases on nutrient digestibility and disappearance of non-starch polysaccharides in broiler chickens. Poult Sci. 2015 Nov;94(11):2662-9. https://doi.org/10.3382/ps/pev260
 
Paiva FC, Alecrim MM, Teixeira MFS, Kirsch LS, Jesus RS. Producao de hidrolisado proteico de pirarucu utilizando-se protease de Aspergillus flavo-furcatis e pancreatina [Production of arapaima protein hydrolysate using Aspergillus flavo-furcatis protease and pancreatin]. Pesq Agropec Trop. 2015 Jan-Mar;45(1):89-96. Portuguese.
 
Pereira CMC, Hannas MI, Rostagno HS, Albino LFT, Balbino EM, Baffa DF, Bernardino VMP. Determination of the ileal digestibility of proteins and amino acids from biscuit bran and wheat gluten in swine. J Appl Life Sci Int. 2017 Jun 19;12(2): [6 p.].
 
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Sakomura NK, Rostagno HS. Metodos de pesquisa em nutricao de monogastrico [Research methods in monogastric traction]. 2nd ed. Jabuticabal, Brazil: Funep; 2007. 262 p. Portuguese.
 
Scotta BA, Albino LFT, Brustolini PC, Gomide APC, Campos PF, Rodrigues VV. Determinacao da composicao quimica e dos valores de energia metabolizavel de alguns alimentos proteicos para frangos de corte [Determination of chemical composition and metabolizable energy values of some protein feeds for broiler chicken]. Cienc Anim Bras. 2016 Oct-Dec;17(4):501-8. Portuguese. https://doi.org/10.1590/1089-6891v17i421347
 
Troni AR, Gomes PC, Mello HHDC, Albino LFT, Rocha TCD. Composicao quimica e energetica de alimentos para frangos de corte [Chemical and energy composition of broiler feeds]. Rev Cienc Agron. 2016 Oct-Dec;47(4):755-60. Portuguese.
 
Tschirner M, Simon A. Influence of different growing substrates and processing on the nutrient composition of black soldier fly larvae destined for animal feed. J Insects as Food Feed. 2015 Dec 7;1(4):249-59.
 
Tuncil YE, Thakkar RD, Arioglu-Tuncil S, Hamaker BR, Lindemann SR. Fecal microbiota responses to bran particles are specific to cereal type and in vitro digestion methods that mimic upper gastrointestinal tract passage. J Agric Food Chem. 2018 Nov 8;66(47):12580-93. https://doi.org/10.1021/acs.jafc.8b03469
 
Vieira RA, Albino LFT, Hannas MI, Viana GS, Muniz JCL, Silva DL, Reis JVC. Composicao quimica e valores de energia metabolizavel aparente corrigida de alguns alimentos energeticos determinados com frango de corte. Rev Bras Agropecu Sustent [Chemical composition and apparent metabolized energy value of some energy feedstuffs for broiler]. 2014 Dec 29;4(2):75-83. Portuguese.
 
Yadav S, Jha R. Strategies to modulate the intestinal microbiota and their effects on nutrient utilization, performance, and health of poultry. J Anim Sci Biotechnol. 2019 Jan 15;10(2): [11 p.].
 
Zhang F, Adeola O. Techniques for evaluating digestibility of energy, amino acids, phosphorus, and calcium in feed ingredients for pigs. Anim Nutr. 2017 Dec;3(4):344-52
 
Amorim AF, Silva GF, Rodrigues KF, Sousa JPL, Soares JAR. Subprodutos utilizados na alimentacao de frangos de corte [By-products used in broiler feed]. PubVet. 2015 May;9(5):195-210. Portuguese.
 
Carvalho CMC, Fernandes EA, Carvalho AP, Caires RM, Fagundes NS. Uso de farinhas de origem animal na alimentacao de frangos de corte [Use of animal meal in broilers feed]. Rev Port Cienc Vet. 2012 Jan-Jun;111(581-2):69-73. Portuguese.
 
Carvalho DP, Leandro NSM, Andrade MA, Oliveira HF, Pires MF, Teixeira KA, Assuncao PS, Stringhini JH. Protease inclusion in plant- and animal-based broiler diets: Performance, digestibility and biometry of digestive organs. S Afr J Anim Sci. 2020 Sep;50(2):291-301. https://doi.org/10.4314/sajas.v50i2.12
 
Cheong CW, Lee YS, Ahmad SA, Ooi PT, Phang LY. Chicken feather valorization by thermal alkaline pretreatment followed by enzymatic hydrolysis for protein-rich hydrolysate production. J Waste Manag. 2018 Sep;79:658-66.
 
Cowieson AJ, Roos FF. Toward optimal value creation through the application of exogenous mono-component protease in the diets of non-ruminants. Anim Feed Sci Tech. 2016 Nov;221:331-40.
 
Detmann E, Souza MA, Valadares Filho SC, Queiroz AC, Berchielli TT, Saliba EOS, Cabral LS, Pina DS, Ladeira MM, Azevedo JAG. Metodos para analise de alimentos do Instituto Nacional de Ciencia e Tecnologia de Ciencia Animal [Methods for food analysis of the National Institute of Science and Technology of Animal Science]. Visconde do Rio Branco, Brazil: Producao Independente; 2012. 214 p. Portuguese.
 
Ferreira DF. SISVAR: Um programa para analises e ensino de estatistica [A program for analysis and teaching of statistics]. Rev Symp. 2008 Jul-Dec; 6:36-41. Portuguese.
 
Gallardo C, Dadalt JC, Kiarie E, Trindade NMAD. Effects of multi-carbohydrase and phytase on standardized ileal digestibility of amino acids and apparent metabolizable energy in canola meal fed to broiler chicks. Poult Sci. 2017 Sep;96(9):3305-13.
 
Mahmood T, Mirza MA, Nawaz H, Shahid M, Athar M, Hussain M. Effect of supplementing exogenous protease in low protein poultry by-product meal based diets on growth performance and nutrient digestibility in broilers. Anim Feed Sci Technol. 2017 Jun;228:23-31.
 
Mahmoudnia N, Boldaji F, Dastar B, Zerehdaran S. Nutritional evaluation of poultry by-product meal in broiler chickens. ABAH Bioflux. 2011 Jun;3(1):55-63.
 
Matias CFQ, Rocha JSR, Pompeu MA, Baiao RC, Baiao NC, Lara LJC, Cardeal PC. Effect of protease on the metabolization coefficient of nutrients in broilers. Arq Bras Med Vet Zootec. 2015 Apr;67(2):492-8. https://doi.org/10.1590/1678-7883
 
Matterson LD, Potter LM, Stutz MW, Singsen EP. The metabolizable energy of feed ingredients for chickens. Res Rep Conn Agric Exp Stn. 1965 Mar;7(1):11-4.
 
Olukosi OA, Beeson LA, Englyst K, Romero LF. Effects of exogenous proteases without or with carbohydrases on nutrient digestibility and disappearance of non-starch polysaccharides in broiler chickens. Poult Sci. 2015 Nov;94(11):2662-9. https://doi.org/10.3382/ps/pev260
 
Paiva FC, Alecrim MM, Teixeira MFS, Kirsch LS, Jesus RS. Producao de hidrolisado proteico de pirarucu utilizando-se protease de Aspergillus flavo-furcatis e pancreatina [Production of arapaima protein hydrolysate using Aspergillus flavo-furcatis protease and pancreatin]. Pesq Agropec Trop. 2015 Jan-Mar;45(1):89-96. Portuguese. https://doi.org/10.1590/1983-40632015v4529838
 
Pereira CMC, Hannas MI, Rostagno HS, Albino LFT, Balbino EM, Baffa DF, Bernardino VMP. Determination of the ileal digestibility of proteins and amino acids from biscuit bran and wheat gluten in swine. J Appl Life Sci Int. 2017 Jun 19;12(2): [6 p.].
 
Perera WNU, Abdollahi MR, Zaefarian F, Wester TJ, Ravindran G, Ravindran V. Influence of inclusion level of barley in wheat-based diets and supplementation of carbohydrase on growth performance, nutrient utilisation and gut morphometry in broiler starters. Br Poult Sci. 2019 Feb;60(6):736-48. https://doi.org/10.1080/00071668.2019.1639142
 
Rostagno HS, Albino LFT, Donzele JL, Oliveira RF, Barreto SLT, Hannas MI, Sakamura NK, Perazzo FG, Saraiva A, Abreu MLT, Rodrigues PB, Brito CO. Tabelas Brasileiras para aves e suinos: Composicao de alimentos e exigencias nutricionais [Brazilian Tables for poultry and swine: Food composition and demand nutritional benefits]. 4th ed. Vicosa, Brazil: Universidade Federal de Vicosa; 2017. 488 p. Portuguese.
 
Sakomura NK, Rostagno HS. Metodos de pesquisa em nutricao de monogastrico [Research methods in monogastric traction]. 2nd ed. Jabuticabal, Brazil: Funep; 2007. 262 p. Portuguese.
 
Scotta BA, Albino LFT, Brustolini PC, Gomide APC, Campos PF, Rodrigues VV. Determinacao da composicao quimica e dos valores de energia metabolizavel de alguns alimentos proteicos para frangos de corte [Determination of chemical composition and metabolizable energy values of some protein feeds for broiler chicken]. Cienc Anim Bras. 2016 Oct-Dec;17(4):501-8. Portuguese. https://doi.org/10.1590/1089-6891v17i421347
 
Troni AR, Gomes PC, Mello HHDC, Albino LFT, Rocha TCD. Composicao quimica e energetica de alimentos para frangos de corte [Chemical and energy composition of broiler feeds]. Rev Cienc Agron. 2016 Oct-Dec;47(4):755-60. Portuguese. https://doi.org/10.5935/1806-6690.20160090
 
Tschirner M, Simon A. Influence of different growing substrates and processing on the nutrient composition of black soldier fly larvae destined for animal feed. J Insects as Food Feed. 2015 Dec 7;1(4):249-59.  https://doi.org/10.3920/JIFF2014.0008
 
Tuncil YE, Thakkar RD, Arioglu-Tuncil S, Hamaker BR, Lindemann SR. Fecal microbiota responses to bran particles are specific to cereal type and in vitro digestion methods that mimic upper gastrointestinal tract passage. J Agric Food Chem. 2018 Nov 8;66(47):12580-93.
 
Vieira RA, Albino LFT, Hannas MI, Viana GS, Muniz JCL, Silva DL, Reis JVC. Composicao quimica e valores de energia metabolizavel aparente corrigida de alguns alimentos energeticos determinados com frango de corte. Rev Bras Agropecu Sustent [Chemical composition and apparent metabolized energy value of some energy feedstuffs for broiler]. 2014 Dec 29;4(2):75-83. Portuguese. https://doi.org/10.21206/rbas.v4i2.261
 
Yadav S, Jha R. Strategies to modulate the intestinal microbiota and their effects on nutrient utilization, performance, and health of poultry. J Anim Sci Biotechnol. 2019 Jan 15;10(2): [11 p.].
 
Zhang F, Adeola O. Techniques for evaluating digestibility of energy, amino acids, phosphorus, and calcium in feed ingredients for pigs. Anim Nutr. 2017 Dec;3(4):344-52 https://doi.org/10.1016/j.aninu.2017.06.008
 
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