Effect of carboxymethyl chito-oligosaccharide on cysteine absorption in intestinal porcine epithelial cells


Huang H.Y., Zhang C., Feng L.Q., Wang N.X., Wang K., Song Z.H., Gan L. (2022): Effect of carboxymethyl chito-oligosaccharide on cysteine absorption in intestinal porcine epithelial cells. Czech J. Anim. Sci., 67: 266–274.

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Oxidative stress is associated with retarded growth and the initiation and progression of diseases in pigs. The carboxymethylation of chito-oligosaccharide (COS) can improve its antioxidant activity for such issues. Herein, an orthogonal experiment L9 (34) test design was used to optimise the preparation conditions of carboxymethyl (CM)-COS. Fourier-transform infrared analysis evidenced the carboxymethylation of COS, and an in vitro study indicated that the hydroxyl radical scavenging ability of CM-COS is superior to that of COS. CM-COS can also better promote the absorption of cysteine and increase the expression of the amino acid transport system b0,+ in intestinal porcine epithelial cells. The results suggested that CM-COS can effectively resist oxidative damage by promoting cysteine absorption mediated by transport system b0,+, which provides important information regarding the antioxidative damage application of COS and CM-COS in the pig farming industry.

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