Effects of dietary fumaric acid on the growth performance, immune response, relative weight and antioxidant status of immune organs in broilers exposed to chronic heat stress

https://doi.org/10.17221/13/2020-CJASCitation:He S., Yin Q., Xiong Y., Liu D., Hu H. (2020): Effects of dietary fumaric acid on the growth performance, immune response, relative weight and antioxidant status of immune organs in broilers exposed to chronic heat stress. Czech J. Anim. Sci., 65: 104-113.
download PDF

This study aimed to investigate the effects of dietary fumaric acid (FA) on growth performance, immune responses, immune organ index and antioxidant status in broilers under chronic heat stress (HS). A total of 200 21-day-old Ross 308 chicks were randomly assigned in a 2 × 2 factorial arrangement with two diets (basal diet or 10 g/kg FA diet) and two temperatures (thermoneutral or HS) for 21 days. On day 42, growth performance, immune organ index, immune function and antioxidative ability were determined. HS resulted in a significant reduction in final body weight (FBW), average daily feed intake (ADFI), average daily gain (ADG), antibody titres against sheep red blood cells (SRBC) and Newcastle disease virus, IgM, IgG, relative weights of spleen, thymus and bursa of Fabricius, but a significant increase in the feed conversion ratio (FCR), activities of total superoxide dismutase (T-SOD) and glutathione peroxidase (GPx) in the bursa, contents of malondialdehyde and total carbonyl (TC) in thymus and bursa (P < 0.05). Dietary supplementation of FA increased FBW, ADFI, ADG, antibody titres against SRBC, IgG, relative weights of spleen and bursa, activity of GPx in thymus and bursa, whereas it decreased the FCR and TC of thymus and bursa. These results suggest that dietary 10 g/kg FA had positive effects on growth performance and immune function through improving the antioxidative capacity of immune organs.

Abudabos AM, Al-Mufarrej SI. Effects of organic acid supplementation on antioxidant capacity and immune responses of broilers challenged orally with Salmonella enterica subsp enteric Typhimurium. S Afr J Anim Sci. 2014 Nov;44(4):342-9.  https://doi.org/10.4314/sajas.v44i4.4
Adil S, Banday T, Bhat GA, Mir MS, Rehman M. Effect of dietary supplementation of organic acids on performance, intestinal histomorphology, and serum biochemistry of broiler chicken. Vet Med Int. 2010 Jun;2010(4):1-7. https://doi.org/10.4061/2010/479485
Aengwanich W. Comparative ability to tolerate heat between Thai indigenous, Thai indigenous crossbred and broilers chickens by using heterophil/lymphocyte ratio. Pak J Biol Sci. 2007 Jun 1;10(11):1840-4. https://doi.org/10.3923/pjbs.2007.1840.1844
Aschbacher K, O’donovan A, Wolkowitz OM, Dhabhar FS, Su Y, Epel E. Good stress, bad stress and oxidative stress: Insights from anticipatory cortisol reactivity. Psychoneuroendocrinology. 2013 Sep 1;38(9):1698-708. https://doi.org/10.1016/j.psyneuen.2013.02.004
Banday MT, Adil S, Khan AA, Untoo M. A study on efficacy of fumaric acid supplementation in diet of broiler chicken. Int J Poultry Sci. 2015 Nov 1;14(11):589-94. https://doi.org/10.3923/ijps.2015.589.594
Collier RJ, Renquist BJ, Xiao YA. 100-Year review: Stress physiology including heat stress. J Dairy Sci. 2017 Dec 1;100(12):10367-80. https://doi.org/10.3168/jds.2017-13676
Durek J, Ghadiri Khozroughi A, Frohling A, Schluter O, Knorr F, Mader A, Goodarzi Boroojeni F, Zentek J, Knorr D, Bolling JS. Effects of thermally treated broiler feed with different organic acid levels on resulting meat composition and parameters related to meat quality. Innov Food Sci Emerg. 2014 Dec 1;26:397-405. https://doi.org/10.1016/j.ifset.2014.05.001
Emami NK, Daneshmand A, Naeini SZ, Graystone EN, Broom LJ. Effects of commercial organic acid blends on male broilers challenged with E.coli K88: performance, microbiology, intestinal morphology, and immune response. Poult Sci. 2017 Sep 1;96(9):3254-63. https://doi.org/10.3382/ps/pex106
Habibu B, Dzenda T, Ayo JO, Yaqub LS, Kawu MU. Haematological changes and plasma fluid dynamics in livestock during thermal stress, and response to mitigative measures. Livest Sci. 2018 Aug 1;214:189-201. https://doi.org/10.1016/j.livsci.2018.05.023
Honda BTB, Calefi AS, Costola-de-Souza C, Quinteiro-Filho WM, da Silva Fonseca JG, De Paula VF, Palermo-Neto J. Effects of heat stress on peripheral T and B lymphocyte profiles and IgG and IgM serum levels in broiler chickens vaccinated for Newcastle disease virus. Poult Sci. 2015 Oct 1;94(10):2375-81. https://doi.org/10.3382/ps/pev192
Kamel NN, Ahmed AMH, Mehaisen GMK, Abass AO. Depression of leukocyte protein synthesis, immune function and growth performance induced by high environmental temperature in broiler chickens. Int J Biometeorol. 2017 Sep 1;61(9):1637-45. https://doi.org/10.1007/s00484-017-1342-0
Khodambashi Emami N, Zafari Naeini S, Ruiz-Feria CA. Growth performance, digestibility, immune response and intestinal morphology of male broilers fed phosphorus deficient diets supplemented with microbial phytase and organic acids. Livest Sci. 2013 Nov 1;157(2-3):506-13. https://doi.org/10.1016/j.livsci.2013.08.014
Lara LJ, Rostagno MH. Impact of heat stress on poultry production. Animals (Basel). 2013 Apr;3(2):356-69. https://doi.org/10.3390/ani3020356
Lentfer TL, Pendl H, Gebhardt-Henrich SG, Frohlich EKF, Von Borell E. H/L ratio as a measurement of stress in laying hens – methodology and reliability. Br Poult Sci. 2015 Apr 4;56(2):157-63. https://doi.org/10.1080/00071668.2015.1008993
Liang C, Xie XZ, Zhou YW, Jiang YY, Xie LJ, Chen Z. Effects of γ-aminobutyric acid on the thymus tissue structure, antioxidant activity, cell apoptosis, and cytokine levels in chicks under heat stress. Czech J Anim Sci. 2016 Nov 28;61(12):539-50. https://doi.org/10.17221/67/2015-CJAS
Liu Y, Yang X, Xin H, Chen S, Yang C, Duan Y, Yang X. Effects of a protected inclusion of organic acids, essential oils as antibiotic growth promoter alternative on growth performance, intestinal morphology, gut microflora in broilers. Anim Sci J. 2017 Sep;88(9):1414-24. https://doi.org/10.1111/asj.12782
Mahmoud KZ, Edens FW. Influence of selenium source on age-related and mild heat stress-related changes of blood and liver glutathione redox cycle in broiler chickens (Gallus domesticus). Comp Biochem Physiol Part B: Biochem Mol Biol. 2003 Dec;136(4):921-34. https://doi.org/10.1016/S1096-4959(03)00288-4
Mirzaie S, Zirak-Khattab F, Hosseini SA, Donyaei-Darian H. Effects of dietary Spirulina on antioxidant status, lipid profile, immune response and performance characteristics of broiler chickens reared under high ambient temperature. Asian-Australas J Anim Sci. 2018 Apr;31(4):556-63. https://doi.org/10.5713/ajas.17.0483
Moharregh-Khiabani D, Linker RA, Gold R, Stangel M. Fumaric acid, its esters: an emerging treatment for multiple sclerosis. Curr Neuropharmacol. 2009 Mar 1;7(1):60-4. https://doi.org/10.2174/157015909787602788
Niu ZY, Liu FZ, Yan QL, Li WC. Effects of different levels of vitamin E on growth performance and immune responses of broilers under heat stress. Poult Sci. 2009 Oct 1;88(10):2101-7. https://doi.org/10.3382/ps.2009-00220
Park SO, Hwangbo J, Ryu C, Park BS, Chae H, Choi H, Kang H, Seo O, Choi Y. Effects of extreme heat stress on growth performance, lymphoid organ, IgG and cecum microflora of broiler chickens. Int J Agric Biol. 2013 Dec;15(6):1204-8.
Pirgozliev V, Murphy TC, Owens B, George J, McCann MEE. Fumaric and sorbic acid as additives in broiler feed. Res Vet Sci. 2008 Jun 1;84(3):387-94. https://doi.org/10.1016/j.rvsc.2007.06.010
Shakeri M, Cottrell JJ, Wilkinson S, Ringue M, Furness JB, Dunshea FR. Betaine and antioxidants improve growth performance, breast muscle development and ameliorate thermoregulatory responses to cyclic heat exposure in broiler chickens. Animals (Basel). 2018 Sep;8(10):162. https://doi.org/10.3390/ani8100162
Shini S, Huff GR, Shini A, Kaiser P. Understanding stress-induced immunosuppression: Exploration of cytokine and chemokine gene profiles in chicken peripheral leukocytes. Poult Sci. 2010 Apr 1;89(4):841-51. https://doi.org/10.3382/ps.2009-00483
Sohail MU, Hume ME, Byrd JA, Nisbet DJ, Ijaz A, Sohail A, Shabbir MZ, Rehman H. Effect of supplementation of prebiotic mannan-oligosaccharides and probiotic mixture on growth performance of broilers subjected to chronic heat stress. Poult Sci. 2012 Sep 1;91(9):2235-40. https://doi.org/10.3382/ps.2012-02182
Yang X, Xin H, Yang C, Yang X. Impact of essential oils and organic acids on the growth performance, digestive functions and immunity of broiler chickens. Anim Nutr. 2018 Dec 1;4(4):388-93. https://doi.org/10.1016/j.aninu.2018.04.005
download PDF

© 2021 Czech Academy of Agricultural Sciences | Prohlášení o přístupnosti