Effect of probiotic Clostridium butyricum CBM 588 on microbiota and growth performance of broiler chickens

https://doi.org/10.17221/143/2019-CJASCitation:Svejstil R., Plachy V., Joch M., Salmonova H., Duskova D., Hautekiet V., Vlkova E. (2019): Effect of probiotic Clostridium butyricum CBM 588 on microbiota and growth performance of broiler chickens. Czech J. Anim. Sci., 64: 387-394.
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Clostridium butyricum CBM 588 is used as a probiotic in eastern Asian countries and has been recently approved as an animal feed additive in the European Union. The purpose of this study was to evaluate the effect of C. butyricum MIYAIRI 588 (CBM 588) on abundance of selected genera of caecal and crop bacteria, volatile fatty acids and growth performance of broiler chickens. We studied counts of anaerobic bacteria in caeca and crops of broiler chickens by plate-count method and evaluated their growth performance. CBM 588 significantly reduced E. coli counts in caeca of broiler chickens at days 10 and 42 and also enhanced their growth performance. Additionally, it significantly increased the amount of butyrate in the caeca that provides energy to enterocytes, resulting in increased weight gains. Out of the obtained results we conclude that C. butyricum CBM 588 influences caecal microbiota of broiler chickens and positively affects their growth performance.

Apata D.F. (2008): Growth performance, nutrient digestibility and immune response of broiler chicks fed diets supplemented with a culture of Lactobacillus bulgaricus. Journal of the Science of Food and Agriculture, 88, 1253–1258.  https://doi.org/10.1002/jsfa.3214
Barrow P.A. (1992): Probiotics for chickens. In: Hill M. (ed.): Probiotics: The Scientific Basis. Springer, Dordrecht, the Netherlands, 225–257.
Cassir N., Benamar S., La Scola B. (2016): Clostridium butyricum: From beneficial to a new emerging pathogen. Clinical Microbiology and Infection, 22, 37–45.  https://doi.org/10.1016/j.cmi.2015.10.014
Corry J.E.K., Curtis G.D.W., Baird R.M. (eds) (2003): Progress in Industrial Microbiology. Elsevier, New York, USA.
Cummings J.H., Macfarlane G.T. (1997): Role of intestinal bacteria in nutrient metabolism. Clinical Nutrition, 16, 3–11.  https://doi.org/10.1016/S0261-5614(97)80252-X
Dho-Moulin M., Fairbrother J.M. (1999): Avian pathogenic Escherichia coli (APEC). Veterinary Research, 30, 299–316.
EFSA (2013): Scientific Opinion on the safety and efficacy of Miya-Gold (Clostridium butyricum) for chickens for fattening, chickens reared for laying and minor avian species. EFSA Journal, 11, 3040.  https://doi.org/10.2903/j.efsa.2013.3040
Fuller R. (2001): The chicken gut microflora and probiotic supplements. The Journal of Poultry Science, 38, 189–196.  https://doi.org/10.2141/jpsa.38.189
Geigerova M., Vlkova E., Bunesova V., Rada V. (2016): Persistence of bifidobacteria in the intestines of calves after administration in freeze-dried form or in fermented milk. Czech Journal of Animal Science, 61, 49–56.  https://doi.org/10.17221/8727-CJAS
Guarner F., Malagelada J.-R. (2003): Gut flora in health and disease. The Lancet, 361, 512–519.  https://doi.org/10.1016/S0140-6736(03)12489-0
Hayes J.R., English L.L., Carr L.E., Wagner D.D., Joseph S.W. (2004): Multiple-antibiotic resistance of Enterococcus spp. isolated from commercial poultry production environments. Applied and Environmental Microbiology, 70, 6005–6011.  https://doi.org/10.1128/AEM.70.10.6005-6011.2004
Hu Z., Guo Y. (2007): Effects of dietary sodium butyrate supplementation on the intestinal morphological structure, absorptive function and gut flora in chickens. Animal Feed Science and Technology, 132, 240–249.  https://doi.org/10.1016/j.anifeedsci.2006.03.017
Imase K., Takahashi M., Tanaka A., Tokunaga K., Sugano H., Tanaka M., Ishida H., Kamiya S., Takahashi S. (2008): Efficacy of Clostridium butyricum preparation concomitantly with Helicobacter pylori eradication therapy in relation to changes in the intestinal microbiota. Microbiology and Immunology, 52, 156–161.  https://doi.org/10.1111/j.1348-0421.2008.00026.x
Islam S.U. (2016): Clinical uses of probiotics. Medicine, 95, 2658.  https://doi.org/10.1097/MD.0000000000002658
Jin L.Z., Ho Y.W., Abdullah N., Jalaludin S. (1998): Growth performance, intestinal microbial populations, and serum cholesterol of broilers fed diets containing Lactobacillus cultures. Poultry Science, 77, 1259–1265.  https://doi.org/10.1093/ps/77.9.1259
Joch M., Kudrna V., Hucko B. (2017): Effects of geraniol and camphene on in vitro rumen fermentation and methane production. Scientia Agriculturae Bohemica, 48, 63–69.  https://doi.org/10.1515/sab-2017-0012
Kalavathy R., Abdullah N., Jalaludin S., Ho Y.W. (2003): Effects of Lactobacillus cultures on growth performance, abdominal fat deposition, serum lipids and weight of organs of broiler chickens. British Poultry Science, 44, 139–144. https://doi.org/10.1080/0007166031000085445
Khaksefidi A., Ghoorchi T. (2006): Effect of probiotic on performance and immunocompetence in broiler chicks. The Journal of Poultry Science, 43, 296–300.  https://doi.org/10.2141/jpsa.43.296
Kohl K.D. (2012): Diversity and function of the avian gut microbiota. Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology, 182, 591–602.  https://doi.org/10.1007/s00360-012-0645-z
Kotunia A., Wolinski J., Laubitz D., Jurkowska M., Rome V., Guilloteau P., Zabielski R. (2004): Effect of sodium butyrate on the small intestine development in neonatal piglets fed by artificial sow. Journal of physiology and pharmacology: an official journal of the Polish Physiological Society, 55 (Suppl. 2), 59–68.
Kuroiwa T., Iwanaga M., Kobari K., Higashionna A., Kinjyo F., Saito A. (1990): Preventive effect of Clostridium butyricum M588 against the proliferation of Clostridium difficile during antimicrobial therapy. Kansenshogaku Zasshi. The Journal of the Japanese Association for Infectious Diseases, 64, 1425–1432.
Lutful Kabir S.M. (2009): The role of probiotics in the poultry industry. International Journal of Molecular Sciences, 10, 3531–3546.  https://doi.org/10.3390/ijms10083531
Manges A.R. (2016): Escherichia coli and urinary tract infections: the role of poultry-meat. Clinical Microbiology and Infection, 22, 122–129.  https://doi.org/10.1016/j.cmi.2015.11.010
Matis G., Neogrady Z., Csiko G., Galfi P., Febel H., Jemnitz K., Veres Z., Kulcsar A., Kenez A., Huber K. (2013): Epigenetic effects of dietary butyrate on hepatic histone acetylation and enzymes of biotransformation in chicken. Acta Veterinaria Hungarica, 61, 477–490.  https://doi.org/10.1556/AVet.2013.033
McLellan S.L., Daniels A.D., Salmore A.K. (2003): Genetic characterization of Escherichia coli populations from host sources of fecal pollution by using DNA fingerprinting. Applied and Environmental Microbiology, 69, 2587–2594.  https://doi.org/10.1128/AEM.69.5.2587-2594.2003
Nicol C.J., Gregory N.G., Knowles T.G., Parkman I.D., Wilkins L.J. (1999): Differential effects of increased stocking density, mediated by increased flock size, on feather pecking and aggression in laying hens. Applied Animal Behaviour Science, 65, 137–152.  https://doi.org/10.1016/S0168-1591(99)00057-X
Niemi R.M., Ahtiainen J. (1995): Enumeration of intestinal enterococci and interfering organisms with Slanetz-Bartley agar, KF streptococcus agar and the MUST method. Letters in Applied Microbiology, 20, 92–97.  https://doi.org/10.1111/j.1472-765X.1995.tb01294.x
Nurmi E., Rantala M. (1973): New aspects of salmonella infection in broiler production. Nature, 241, 210–211.  https://doi.org/10.1038/241210a0
Okamoto T., Sasaki M., Tsujikawa T., Fujiyama Y., Bamba T., Kusunoki M. (2000): Preventive efficacy of butyrate enemas and oral administration of Clostridium butyricum M588 in dextran sodium sulfate-induced colitis in rats. Journal of Gastroenterology, 35, 341–346.  https://doi.org/10.1007/s005350050358
Owings W.J., Reynolds D.L., Hasiak R.J., Ferket P.R. (1990): Influence of dietary supplementation with Streptococcus faecium M-74 on broiler body weight, feed conversion, carcass characteristics, and intestinal microbial colonization. Poultry Science, 69, 1257–1264.  https://doi.org/10.3382/ps.0691257
Rada V., Petr J. (2000): A new selective medium for the isolation of glucose non-fermenting bifidobacteria from hen caeca. Journal of Microbiological Methods, 43, 127–132. https://doi.org/10.1016/S0167-7012(00)00205-0
Roediger W. (1995): The place of short-chain fatty acids in colonocyte metabolism in health and ulcerative colitis: The impaired colonocyte barrier. In: Cummings J., Rombeau J., Sakata T. (eds): Physiological and Clinical Aspects of Short-chain Fatty Acids. Cambridge University Press, Cambridge, UK, 337–387.
Seki H., Shiohara M., Matsumura T., Miyagawa N., Tanaka M., Komiyama A., Kurata S. (2003): Prevention of antibiotic-associated diarrhea in children by Clostridium butyricum MIYAIRI. Pediatrics International, 45, 86–90.  https://doi.org/10.1046/j.1442-200X.2003.01671.x
Shimbo I., Yamaguchi T., Odaka T., Nakajima K., Koide A., Koyama H., Saisho H. (2005): Effect of Clostridium butyricum on fecal flora in Helicobacter pylori eradication therapy. World Journal of Gastroenterology, 11, 7520–7524.  https://doi.org/10.3748/wjg.v11.i47.7520
Stepien-Pysniak D., Marek A., Banach T., Adaszek L., Pyzik E., Wilczynski J., Winiarczyk S. (2016): Prevalence and antibiotic resistance of Enterococcus strains isolated from poultry. Acta Veterinaria Hungarica, 64, 148–163.  https://doi.org/10.1556/004.2016.016
Takahashi M., Taguchi H., Yamaguchi H., Osaki T., Komatsu A., Kamiya S. (2004): The effect of probiotic treatment with Clostridium butyricum on enterohemorrhagic Escherichia coli O157:H7 infection in mice. FEMS Immunology and Medical Microbiology, 41, 219–226.  https://doi.org/10.1016/j.femsim.2004.03.010
Varmuzova K., Kubasova T., Davidova-Gerzova L., Sisak F., Havlickova H., Sebkova A., Faldynova M., Rychlik I. (2016): Composition of gut microbiota influences resistance of newly hatched chickens to Salmonella enteritidis infection. Frontiers in Microbiology, 7, 957.  https://doi.org/10.3389/fmicb.2016.00957
Verhaegen B., De Reu K., Heyndrickx M., De Zutter L. (2015): Comparison of six chromogenic agar media for the isolation of a broad variety of non-O157 shigatoxin-producing Escherichia coli (STEC) serogroups. International Journal of Environmental Research and Public Health, 12, 6965–6978.  https://doi.org/10.3390/ijerph120606965
Vispo C., Karasov W.H. (1997): The interaction of avian gut microbes and their host: An elusive symbiosis. In: Mackie R.I., White B.A. (eds): Gastrointestinal Microbiology: Volume 1 Gastrointestinal Ecosystems and Fermentations. Springer US, Boston, USA, 116–155.
Vlkova E., Salmonova H., Bunesova V., Geigerova M., Rada V., Musilova S. (2015): A new medium containing mupirocin, acetic acid, and norfloxacin for the selective cultivation of bifidobacteria. Anaerobe, 34, 27–33.  https://doi.org/10.1016/j.anaerobe.2015.04.001
Wang Y., Sun J., Zhong H., Li N., Xu H., Zhu Q., Liu Y. (2017): Effect of probiotics on the meat flavour and gut microbiota of chicken. Scientific Reports, 7, 6400.  https://doi.org/10.1038/s41598-017-06677-z
Wilkins T.D., Chalgren S. (1976): Medium for use in antibiotic susceptibility testing of anaerobic bacteria. Antimicrobial Agents and Chemotherapy, 10, 926–928.  https://doi.org/10.1128/AAC.10.6.926
Yang C.M., Cao G.T, Ferket P.R., Liu T.T, Zhou L., Zhang L., Xiao Y.P., Chen A.G. (2012): Effects of probiotic, Clostridium butyricum, on growth performance, immune function, and cecal microflora in broiler chickens. Poultry Science, 91, 2121–2129.  https://doi.org/10.3382/ps.2011-02131
Zhang B., Yang X., Guo Y., Long F. (2011): Effects of dietary lipids and Clostridium butyricum on the performance and the digestive tract of broiler chickens. Archives of Animal Nutrition, 65, 329–339.  https://doi.org/10.1080/1745039X.2011.568274
Zhang L., Cao G.T., Zeng X.F., Zhou L., Ferket P.R., Xiao Y.P., Chen A.G., Yang C.M. (2014): Effects of Clostridium butyricum on growth performance, immune function, and cecal microflora in broiler chickens challenged with Escherichia coli K88. Poultry Science, 93, 46–53. https://doi.org/10.3382/ps.2013-03412
Zhang L., Zhang L., Zhan X., Zeng X., Zhou L., Cao G., Chen A., Yang C. (2016): Effects of dietary supplementation of probiotic, Clostridium butyricum, on growth performance, immune response, intestinal barrier function, and digestive enzyme activity in broiler chickens challenged with Escherichia coli K88. Journal of Animal Science and Biotechnology, 7, 3.  https://doi.org/10.1186/s40104-016-0061-4
Zhang W.H., Jiang Y., Zhu Q.F., Gao F., Dai S.F., Chen J., Zhou G.H. (2011): Sodium butyrate maintains growth performance by regulating the immune response in broiler chickens. British Poultry Science, 52, 292–301.  https://doi.org/10.1080/00071668.2011.578121
Zhi S., Li Q., Yasui Y., Edge T., Topp E., Neumann N.F. (2015): Assessing host-specificity of Escherichia coli using a supervised learning logic-regression-based analysis of single nucleotide polymorphisms in intergenic regions. Molecular Phylogenetics and Evolution, 92, 72–81. https://doi.org/10.1016/j.ympev.2015.06.007
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