Effects of dietary manganese on antioxidant status, biochemical parameters and thickness of intestinal mucus in laying hens

https://doi.org/10.17221/148/2018-CJASCitation:Piešová E., Faixová Z., Maková Z., Venglovská K., Grešáková Ľ., Faix Š., Čobanová K. (2019): Effects of dietary manganese on antioxidant status, biochemical parameters and thickness of intestinal mucus in laying hens. Czech J. Anim. Sci., 64: 99-106.
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The objective of this study was to investigate the effects of different sources of manganese (Mn) on the antioxidant status, some biochemical parameters and thickness of intestinal mucus in laying hens. Ninety-six 20-week-old hens of the Lohman Brown strain were randomly divided into four groups; each group was replicated six times with four hens per replicate. Hens in the control group were fed the basal diet (BD) with natural background Mn level of 45.5 mg/kg feed. For the three experimental groups, the BD was supplemented with 120 mg Mn/kg either from Mn-sulphate or Mn-chelate of protein hydrolysate (Mn-Bioplex) or Mn-chelate of glycine hydrate (Mn-Glycinoplex). After eight weeks of dietary treatment, a tendency towards increasing activity of superoxide dismutase (SOD) in the erythrocytes was observed in all experimental groups. The results showed that inorganic Mn source increased the glutathione peroxidase activity in the hens’ blood (P < 0.05). The intake of Mn-Bioplex resulted in significantly increased total antioxidant status in the plasma of hens in the group (P < 0.05) compared to the control group. The plasma biological antioxidant potential was increased in the treatment groups supplemented with Mn-sulphate (P < 0.01) and Mn-Bioplex (P < 0.05). The total cholesterol concentration was decreased (P < 0.05) in the group fed the diet supplemented with Mn-Glycinoplex, as compared with the control. Moreover, the mucus layer thickness in the jejunum was reduced in the groups of birds fed with the organic form of Mn (P < 0.001, P < 0.05), as well as with the inorganic form of Mn (P < 0.001). These results indicate that the addition of dietary Mn was effective in improving the biological antioxidant potential, decreasing total plasma cholesterol and reducing the thickness of adherent intestinal mucus gel in the jejunum of laying hens.

Attia Y.A., Qota E.M., Bovera F., Tag El-Din A.E., Mansour S.A. (2010): Effect of amount and source of manganese and/or phytase supplementation on productive and reproductive performance and some physiological traits of dual purpose cross-bred hens in the tropics. British Poultry Science, 51, 235-245  https://doi.org/10.1080/00071661003786111
Bae Yun-Jung, Choi Mi-Kyeong, Kim Mi-Hyun (2011): Manganese Supplementation Reduces the Blood Cholesterol Levels in Ca-Deficient Ovariectomized Rats. Biological Trace Element Research, 141, 224-231  https://doi.org/10.1007/s12011-010-8714-1
Bai S. P., Lu L., Luo X. G., Liu B. (2008): Kinetics of Manganese Absorption in Ligated Small Intestinal Segments of Broilers. Poultry Science, 87, 2596-2604  https://doi.org/10.3382/ps.2008-00117
BAKER D. H., HALPIN K. M. (1987): Research Note: Efficacy of a Manganese-Protein Chelate Compared with that of Manganese Sulfate for Chicks. Poultry Science, 66, 1561-1563  https://doi.org/10.3382/ps.0661561
Bao Y. M., Choct M., Iji P. A., Bruerton K. (2007): Effect of Organically Complexed Copper, Iron, Manganese, and Zinc on Broiler Performance, Mineral Excretion, and Accumulation in Tissues. The Journal of Applied Poultry Research, 16, 448-455  https://doi.org/10.1093/japr/16.3.448
Bomb B.S., Kumawat D.C., Bomb P., Taly A.B., Bedi T., Bedi H.K. (1988): Effect of manganese on regression of atherosclerosis in cholesterol fed rabbits. Journal of the Association of Physicians of India, 36, 149–150.
Bradford Marion M. (1976): A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72, 248-254  https://doi.org/10.1016/0003-2697(76)90527-3
BÜLBÜL Aziz, BÜLBÜL Tuba, KÜÇÜKERSAN Seher, ŞİRELİ Meltem, ERYAVUZ Abdullah (2008): Yumurtacı Tavuklarda Yeme Organik ve İnorganik Zn, Cu ve Mn İlavesinin Oksidan/Antioksidan Denge Üzerine Etkileri. Kafkas Universitesi Veteriner Fakultesi Dergisi, , -  https://doi.org/10.9775/kvfd.2007.36-A
Burlet Elodie, Jain Sushil K. (2013): Manganese Supplementation Reduces High Glucose-induced Monocyte Adhesion to Endothelial Cells and Endothelial Dysfunction in Zucker Diabetic Fatty Rats. Journal of Biological Chemistry, 288, 6409-6416  https://doi.org/10.1074/jbc.M112.447805
Cowieson A.J., Acamovic T., Bedford M.R. (2004): The effects of phytase and phytic acid on the loss of endogenous amino acids and minerals from broiler chickens. British Poultry Science, 45, 101-108  https://doi.org/10.1080/00071660410001668923
Davidsson L, Cederblad A, Lönnerdal B, Sandström B (1991): The effect of individual dietary components on manganese absorption in humans. The American Journal of Clinical Nutrition, 54, 1065-1070  https://doi.org/10.1093/ajcn/54.6.1065
Davis Cindy D., Ney Denise M., Greger J. L. (1990): Manganese, Iron and Lipid Interactions in Rats. The Journal of Nutrition, 120, 507-513  https://doi.org/10.1093/jn/120.5.507
Finley John W., Monroe Patrick (1997): Mn absorption: The use of CACO-2 cells as a model of the intestinal epithelia. The Journal of Nutritional Biochemistry, 8, 92-101  https://doi.org/10.1016/S0955-2863(96)00180-5
Fly Alyce D., Izquierdo Oscar A., Lowry Karen R., Baker David H. (1989): Manganese bioavailability in a MN-methionine chelate. Nutrition Research, 9, 901-910  https://doi.org/10.1016/S0271-5317(89)80035-1
Fouad A.M., Li Y., Chen W., Ruan D., Wang S., Xie W., Lin Y.C., Zheng C.T. (2016): Effects of Dietary Manganese Supplementation on Laying Performance, Egg Quality and Antioxidant Status in Laying Ducks. Asian Journal of Animal and Veterinary Advances, 11, 570-575  https://doi.org/10.3923/ajava.2016.570.575
Fridovich Irwin (1997): Superoxide Anion Radical (O·̄ 2 ), Superoxide Dismutases, and Related Matters. Journal of Biological Chemistry, 272, 18515-18517  https://doi.org/10.1074/jbc.272.30.18515
Garcia-Aranda Jose A., Wapnir Raul A., Lifshitz Fima (1983): In Vivo Intestinal Absorption of Manganese in the Rat. The Journal of Nutrition, 113, 2601-2607  https://doi.org/10.1093/jn/113.12.2601
Ibrahim Mohamed Yousif, Hashim Najihah Mohd, Dhiyaaldeen Summaya M., Al-Obaidi Mazen M.Jamil, El-Ferjani Rashd M., Adam Hoyam, Alkotaini Bassam, Batran Rami Al, Ali Hapipah Mohd (2016): Acute Toxicity and Gastroprotection Studies of a New Schiff Base Derived Manganese (II) Complex against HCl/Ethanol-Induced Gastric Ulcerations in Rats. Scientific Reports, 6, -  https://doi.org/10.1038/srep26819
Jeurissen S.H.M., Lewis F., van der Klis J.D., Mroz Z., Rebel J.M.J., ter Huurne A.A.H.M. (2002): Parameters and techniques to determine intestinal health of poultry as constituted by immunity, integrity, and functionality. Current Issues in Intestinal Microbiology, 3, 1–14.
Ji F., Luo X. G., Lu L., Liu B., Yu S. X. (2006): Effects of manganese source and calcium on manganese uptake by in vitro everted gut sacs of broilers' intestinal segments. Poultry Science, 85, 1217-1225  https://doi.org/10.1093/ps/85.7.1217
Jo C., Ahn D. U. (1998): Fluorometric analysis of 2-thiobarbituric acid reactive substances in turkey. Poultry Science, 77, 475-480  https://doi.org/10.1093/ps/77.3.475
Keen Carl L., Bell Janet G., Lönnerdal Bo (1986): The Effect of Age on Manganese Uptake and Retention from Milk and Infant Formulas in Rats. The Journal of Nutrition, 116, 395-402  https://doi.org/10.1093/jn/116.3.395
Kiela Pawel R., Ghishan Fayez K. (2016): Physiology of Intestinal Absorption and Secretion. Best Practice & Research Clinical Gastroenterology, 30, 145-159  https://doi.org/10.1016/j.bpg.2016.02.007
Leach R.M., Harris E.D. (1997): Manganese. In: O’Dell B.L. and Sunde R.A. (eds): Handbook of Nutritionally Essential Minerals. Marcel Dekker, Inc, New York, USA, 335–355.
Leeson S. (2003): A new look at trace mineral nutrition of poultry: Can we reduce the environmental burden of poultry manure? In: Lyons T.P. and Jacques K.A. (eds): Nutritional Biotechnology in the Feed and Food Industries. Nottingham University Press, Nottingham, UK, 125–129.
Lu L., Ji C., Luo X.G., Liu B., Yu S.X. (2006): The effect of supplemental manganese in broiler diets on abdominal fat deposition and meat quality. Animal Feed Science and Technology, 129, 49-59  https://doi.org/10.1016/j.anifeedsci.2005.12.005
Lu L., Luo X. G., Ji C., Liu B., Yu S. X. (2007): Effect of manganese supplementation and source on carcass traits, meat quality, and lipid oxidation in broilers1. Journal of Animal Science, 85, 812-822  https://doi.org/10.2527/jas.2006-229
Luo X.G., Su Q., Huang J.C., Liu J.X. (1992): Effects of manganese (Mn) deficiency on tissue Mn-containing superoxide dismutase (MnSOD) activity and its mitochondrial ultrastructures of broiler chicks fed a practical diet. Chinese Journal of Animal and Veterinary Science, 23, 97–101.
National Research Council (1994): Nutrient Requirements of Poultry. 9th Ed. The National Academies Press, Washington, USA.
Paglia D.E., Valentine W.M. (1967): Studies on quantitative and qualitative characterization of erythrocyte glutathione peroxidase. Journal of Laboratory and Clinical Medicine, 70, 158–169.
Quarterman J. (2004): Metal Absorption and the Intestinal Mucus Layer. Digestion, 37, 1-9  https://doi.org/10.1159/000199480
Rossander-Hultén L, Brune M, Sandström B, Lönnerdal B, Hallberg L (1991): Competitive inhibition of iron absorption by manganese and zinc in humans. The American Journal of Clinical Nutrition, 54, 152-156  https://doi.org/10.1093/ajcn/54.1.152
Ruskovska Tatjana, Jansen Eugene H. J. M., Antarorov Risto (2014): Evaluation of Assays for Measurement of Serum (Anti)oxidants in Hemodialysis Patients. BioMed Research International, 2014, 1-8  https://doi.org/10.1155/2014/843157
Sahagian Benjamin M., Harding-Barlow I., Perry H. Mitchell (1966): Uptakes of Zinc, Manganese, Cadmium and Mercury by Intact Strips of Rat Intestine. The Journal of Nutrition, 90, 259-267  https://doi.org/10.1093/jn/90.3.259
Samouilidou E., Grapsa E., Karpouza A., Lagouranis A. (2007): Reactive Oxygen Metabolites: A Link between Oxidative Stress and Inflammation in Patients on Hemodialysis. Blood Purification, 25, 175-178  https://doi.org/10.1159/000098521
Saripinar-Aksu D., Aksu T., Ozsoy B. (2010): The effects of lower supplementation levels of organically complexed minerals (zinc, copper and manganese) versus inorganic forms on haematological and biochemical parameters in broilers. Kafkas Universitesi Veteriner Fakultesi Dergisi, 16, 553–559.
Sarsour E. H., Kalen A. L., Xiao Z., Veenstra T. D., Chaudhuri L., Venkataraman S., Reigan P., Buettner G. R., Goswami P. C. (2012): Manganese Superoxide Dismutase Regulates a Metabolic Switch during the Mammalian Cell Cycle. Cancer Research, 72, 3807-3816  https://doi.org/10.1158/0008-5472.CAN-11-1063
Satchithanandam Subramaniam, Vargofcak-Apker Mary, Calvert Richard J., Leeds Anthony R., Cassidy Marie M. (1990): Alteration of Gastrointestinal Mucin by Fiber Feeding in Rats. The Journal of Nutrition, 120, 1179-1184  https://doi.org/10.1093/jn/120.10.1179
Smirnov Asya, Sklan David, Uni Zehava (2004): Mucin Dynamics in the Chick Small Intestine Are Altered by Starvation. The Journal of Nutrition, 134, 736-742  https://doi.org/10.1093/jn/134.4.736
Thompson K. L., Applegate T. J. (2006): Feed Withdrawal Alters Small-Intestinal Morphology and Mucus of Broilers. Poultry Science, 85, 1535-1540  https://doi.org/10.1093/ps/85.9.1535
Wedekind Karen J., Titgemeyer Evan C., Twardock A. Robert, Baker David H. (1991): Phosphorus, but not Calcium, Affects Manganese Absorption and Turnover in Chicks. The Journal of Nutrition, 121, 1776-1786  https://doi.org/10.1093/jn/121.11.1776
Xiao J. F., Wu S. G., Zhang H. J., Yue H. Y., Wang J., Ji F., Qi G. H. (2015): Bioefficacy comparison of organic manganese with inorganic manganese for eggshell quality in Hy-Line Brown laying hens. Poultry Science, 94, 1871-1878  https://doi.org/10.3382/ps/pev138
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