Apoptosis of porcine Sertoli cells is inhibited by QKI-5 via regulating CASP8

https://doi.org/10.17221/158/2018-CJASCitation:Liang M., Liu X., Guo J., Yang Y., Zhang Y., Yu X., Sun B. (2019): Apoptosis of porcine Sertoli cells is inhibited by QKI-5 via regulating CASP8. Czech J. Anim. Sci., 64: 207-215.
supplementary materialdownload PDF

QKI, a KH domain containing RNA binding, is an RNA-binding protein that is involved in cell proliferation and apoptosis through binding to the QKI response element (QRE) site of its target mRNA. And Caspase 8 (CASP8) and Caspase 3 (CASP3) play important roles in the pathway of apoptosis. The purpose of this study was to investigate the effect of QKI-5 on the apoptosis of Sertoli cells. The experimental results show that pig tissues contain QKI-5, QKI-6 and QKI-7. Overexpression of QKI-5 significantly decreased the mRNA expression of CASP8 (P < 0.05) and the protein expression of CASP8 (P < 0.05). On the contrary, inhibiting QKI-5 increased the expression of CASP8 significantly. Overexpression of QKI-5 significantly reduced the apoptosis of Sertoli cells and promoted cell growth (P < 0.05). Furthermore, QKI-5 specifically reduced the stability of CASP8 mRNA by binding QRE sites on CASP8. Our experiments provide preliminary evidence that QKI-5 induces Sertoli cells proliferation by inhibiting apoptosis, and this may be one of the factors promoting testicular development.

Aranha I., Bhagya M., Yajurvedi H.N. (2006): Testis of the lizard Mabuya carinata: A light microscopic and ultrastructural seasonal study. Journal of Submicroscopic Cytology and Pathology, 38, 93–102.
Galarneau André, Richard Stéphane (2005): Target RNA motif and target mRNAs of the Quaking STAR protein. Nature Structural & Molecular Biology, 12, 691-698 https://doi.org/10.1038/nsmb963
Gavino Christina, Richard Stéphane (2011): Loss of p53 in quaking viable mice leads to Purkinje cell defects and reduced survival. Scientific Reports, 1, - https://doi.org/10.1038/srep00084
Geens Mieke, Sermon Karen Dora, Van de Velde Hilde, Tournaye Herman (2011): Sertoli cell-conditioned medium induces germ cell differentiation in human embryonic stem cells. Journal of Assisted Reproduction and Genetics, 28, 471-480 https://doi.org/10.1007/s10815-011-9541-9
Guo Wangang, Shi Xiaoqin, Liu Anheng, Yang Guodong, Yu Fang, Zheng Qiangsun, Wang Zikuan, Allen David G., Lu Zifan (2011): RNA Binding Protein QKI Inhibits the Ischemia/reperfusion-induced Apoptosis in Neonatal Cardiomyocytes. Cellular Physiology and Biochemistry, 28, 593-602 https://doi.org/10.1159/000335755
Hall M. P., Nagel R. J., Fagg W. S., Shiue L., Cline M. S., Perriman R. J., Donohue J. P., Ares M. (2013): Quaking and PTB control overlapping splicing regulatory networks during muscle cell differentiation. RNA, 19, 627-638 https://doi.org/10.1261/rna.038422.113
Lu Wei, Feng Feixue, Xu Jinke, Lu Xiaozhao, Wang Shan, Wang Lifeng, Lu Huanyu, Wei Mengying, Yang Guodong, Wang Li, Lu Zifan, Liu Yanpu, Lei Xiaoying (2014): QKI impairs self-renewal and tumorigenicity of oral cancer cells via repression of SOX2. Cancer Biology & Therapy, 15, 1174-1184 https://doi.org/10.4161/cbt.29502
Mezquita J., Pau M., Mezquita C. (1998): Four isoforms of the signal-transduction and RNA-binding protein QKI expressed during chicken spermatogenesis. Molecular Reproduction and Development, 50, 70-78 https://doi.org/10.1002/(SICI)1098-2795(199805)50:1<70::AID-MRD9>3.0.CO;2-U
Murata Tomoaki, Yamashiro Yasuhiro, Kondo Tatsuya, Nakaichi Munekazu, Une Satoshi, Taura Yasuho (2009): Nucleotide sequence of complementary DNA encoding for quaking protein of cow, horse and pig. DNA Sequence, 16, 300-303 https://doi.org/10.1080/10425170500136731
Oliva Esther, Alvarez Teresa, Young Robert H (2005): Sertoli Cell Tumors of the Ovary. The American Journal of Surgical Pathology, 29, 143-156 https://doi.org/10.1097/01.pas.0000149692.21205.9c
van der Veer Eric P., de Bruin Ruben G., Kraaijeveld Adriaan O., de Vries Margreet R., Bot Ilze, Pera Tonio, Segers Filip M., Trompet Stella, van Gils Janine M., Roeten Marko K., Beckers Cora M., van Santbrink Peter J., Janssen Anique, van Solingen Coen, Swildens Jim, de Boer Hetty C., Peters Erna A., Bijkerk Roel, Rousch Mat, Doop Merijn, Kuiper Johan, Schalij Martin Jan, van der Wal Allard C., Richard Stéphane, van Berkel Theo J.C., Pickering J. Geoffrey, Hiemstra Pieter S., Goumans Marie Jose, Rabelink Ton J., de Vries Antoine A.F., Quax Paul H.A., Jukema J. Wouter, Biessen Erik A.L., van Zonneveld Anton Jan (2013): Quaking, an RNA-Binding Protein, Is a Critical Regulator of Vascular Smooth Muscle Cell Phenotype. Circulation Research, 113, 1065-1075 https://doi.org/10.1161/CIRCRESAHA.113.301302
Wang Yunling, Lacroix Geneviève, Haines Jeffery, Doukhanine Evgueni, Almazan Guillermina, Richard Stéphane, Feng Yue (2010): The QKI-6 RNA Binding Protein Localizes with the MBP mRNAs in Stress Granules of Glial Cells. PLoS ONE, 5, e12824- https://doi.org/10.1371/journal.pone.0012824
Wang Yunling, Vogel Gillian, Yu Zhenbao, Richard Stéphane (2013): The QKI-5 and QKI-6 RNA Binding Proteins Regulate the Expression of MicroRNA 7 in Glial Cells. Molecular and Cellular Biology, 33, 1233-1243 https://doi.org/10.1128/MCB.01604-12
YU FANG, JIN LIANG, YANG GUODONG, JI LIN, WANG FENG, LU ZIFAN (2014): Post-transcriptional repression of FOXO1 by QKI results in low levels of FOXO1 expression in breast cancer cells. Oncology Reports, 31, 1459-1465 https://doi.org/10.3892/or.2013.2957
Zearfoss N. Ruth, Clingman Carina C., Farley Brian M., McCoig Lisa M., Ryder Sean P., Orr Harry T. (2011): Quaking Regulates Hnrnpa1 Expression through Its 3′ UTR in Oligodendrocyte Precursor Cells. PLoS Genetics, 7, e1001269- https://doi.org/10.1371/journal.pgen.1001269
Zhang Shun, Zeng Yi, Qu Juan, Luo Yaning, Wang Xiaohong, Li Wei (2013): Endogenous EGF maintains Sertoli germ cell anchoring junction integrity and is required for early recovery from acute testicular ischemia/reperfusion injury. REPRODUCTION, 145, 177-189 https://doi.org/10.1530/REP-12-0336
Zhang Xiaojun, Li Chuanmin, Liu Xin, Lu Chunyan, Bai Chunyan, Zhao Zhihui, Sun Boxing (2015): Differential Expression of miR-499 and Validation of Predicted Target Genes in the Testicular Tissue of Swine at Different Developmental Stages. DNA and Cell Biology, 34, 464-469 https://doi.org/10.1089/dna.2014.2728
Zhao L., Mandler M. D., Yi H., Feng Y. (2010): Quaking I controls a unique cytoplasmic pathway that regulates alternative splicing of myelin-associated glycoprotein. Proceedings of the National Academy of Sciences, 107, 19061-19066 https://doi.org/10.1073/pnas.1007487107
Zong Feng-Yang, Fu Xing, Wei Wen-Juan, Luo Ya-Ge, Heiner Monika, Cao Li-Juan, Fang Zhaoyuan, Fang Rong, Lu Daru, Ji Hongbin, Hui Jingyi, Cheung Vivian G. (2014): The RNA-Binding Protein QKI Suppresses Cancer-Associated Aberrant Splicing. PLoS Genetics, 10, e1004289- https://doi.org/10.1371/journal.pgen.1004289
supplementary materialdownload PDF

© 2019 Czech Academy of Agricultural Sciences