Influence of interspecific hybridization on fitness-related traits in Siberian sturgeon and Russian sturgeon S., Vuong D.T., Havelka M., Šachlová H., Lebeda I., Kašpar V., Flajšhans M. (2019): Influence of interspecific hybridization on fitness-related traits in Siberian sturgeon and Russian sturgeon. Czech J. Anim. Sci., 64: 78-88.
download PDF

Polyploidy in sturgeons makes them highly susceptible to interspecific hybridization, and these interspecific hybrids have been described in nature as well as in captivity. Nevertheless, the fitness-related traits between sturgeon hybrids and pure species have been poorly compared as yet. In the present study, we compared the reproductive parameters such as fertilization rate and hatching rate, growth traits and genetic polymorphism in the artificially produced hybrids of the Siberian sturgeon (Acipenser baerii) and Russian sturgeon (A. gueldenstaedtii) with their purebreds. Fertilization and hatching rates were found to be significantly higher in Siberian sturgeon (♀) × Russian sturgeon (♂) hybrid group compared to purebreds. The highest cumulative survival rate was determined in purebred groups until 151 days post-hatch (dph); however, this trend changed and Russian sturgeon purebred showed the lowest cumulative survival rate (0.21%) by 913 dph. Similarly, the lowest average body weight was recorded in Russian sturgeon purebred group (264 g). In contrast, the highest average body weight was recorded in Russian sturgeon (♀) × Siberian sturgeon (♂) hybrids (435.3 g) and the highest cumulative survival rate was recorded in Siberian sturgeon (♀) × Russian sturgeon (♂) hybrids (12.32%) by 913 dph. No significant differences were found at heterozygosity levels among studied crosses. Our results showed that studied sturgeon hybrids had higher survival and growth if compared with the purebreds under provided hatchery conditions.

Abbott R., Albach D., Ansell S., Arntzen J. W., Baird S. J. E., Bierne N., Boughman J., Brelsford A., Buerkle C. A., Buggs R., Butlin R. K., Dieckmann U., Eroukhmanoff F., Grill A., Cahan S. H., Hermansen J. S., Hewitt G., Hudson A. G., Jiggins C., Jones J., Keller B., Marczewski T., Mallet J., Martinez-Rodriguez P., Möst M., Mullen S., Nichols R., Nolte A. W., Parisod C., Pfennig K., Rice A. M., Ritchie M. G., Seifert B., Smadja C. M., Stelkens R., Szymura J. M., Väinölä R., Wolf J. B. W., Zinner D. (2013): Hybridization and speciation. Journal of Evolutionary Biology, 26, 229-246
Allendorf F.W., Waples R.S. (1996): Conservation and genetics of salmonid fishes. In: Avise J.C. and Hamrick J.L. (eds): Conservation Genetics: Case Histories from Nature. Springer, New York, USA, 238–280.
Arefjev V.A. (1999): CYTOGENETICS OF INTERPLOID HYBRIDIZATION OF STURGEONS. Journal of Applied Ichthyology, 15, 277-277
Arnold M.L. (1997): Natural Hybridization and Evolution. Oxford University Press, Oxford, UK.
Bartley D.M., Rana K., Immink A.J. (2001): The use of inter-specific hybrids in aquaculture and fisheries. Reviews in Fish Biology and Fisheries, 10, 325–337.
Barulin N.V., Mamedov R.A., Lashkevich A.I. (2008): Hybrid Acipenser gueldenstaedtii × Acipenser baerii a prospective object of sturgeon culture. In: Proc. Internat. Research and Practice Conference: Strategy of Aquaculture Development under Current Conditions. Minsk, USSR, 24, 46–51.
Billard R., Lecointre G. (2000): Biology and conservation of sturgeon and paddlefish. Reviews in Fish Biology and Fisheries, 10, 355–392.
Birstein V.J., Hanner R., DeSalle R. (1997): Phylogeny of the Acipenseriformes: Cytogenetic and molecular approaches. In: Birstein V.J., Waldman J.R., Bemis W.E. (eds): Sturgeon Biodiversity and Conservation. Kluwer Academic Publishers, Dordrecht, the Netherlands, 127–155.
Birstein V.J., Ruban G., Ludwig A., Doukakis P., DeSalle R. (2005): The enigmatic Caspian Sea Russian sturgeon: How many cryptic forms does it contain? Systematics and Biodiversity, 3, 203–218.
Börk Karrigan, Drauch A., Israel J. A., Pedroia J., Rodzen J., May B. (2008): Development of new microsatellite primers for green and white sturgeon. Conservation Genetics, 9, 973-979
Boscari E., Barmintseva A., Pujolar J. M., Doukakis P., Mugue N., Congiu L. (2014): Species and hybrid identification of sturgeon caviar: a new molecular approach to detect illegal trade. Molecular Ecology Resources, 14, 489-498
Bronzi P., Rosenthal H., Arlati G, Williot P. (1999): A brief overview on the status and prospects of sturgeon farming in Western and Central Europe. Journal of Applied Ichthyology, 15, 224-227
Campton D.E. (1987): Natural hybridization and introgression in fishes: Methods of detection and interpretation. In: Ryman N. and Utter F.M. (eds): Population Genetics and Fishery Management. University of Washington Press, Seattle, USA, 161–192.
Chebanov M.S., Galich E.V. (2011): Sturgeon Hatchery Manual. Available at (accessed July 10, 2018).
Chevassus B. (1983): Hybridization in fish. Aquaculture, 33, 245-262
Dettlaff T.A., Ginsburg A.S., Schmalhausen O.I. (2012): Sturgeon fishes: Developmental biology and aquaculture. Springer Science and Business Media.
deWet J. M. J., Fletcher G. B., Hilu K. W., Harlan J. R. (1983): ORIGIN OF TRIPSACUM ANDERSONII (GRAMINEAE). American Journal of Botany, 70, 706-711
Facon Benoît, Pointier Jean-Pierre, Jarne Philippe, Sarda Violette, David Patrice (2008): High Genetic Variance in Life-History Strategies within Invasive Populations by Way of Multiple Introductions. Current Biology, 18, 363-367
Danzmann Roy, Fontana Francesco, Congiu Leonardo, Mudrak Vincent A., Quattro Joseph M., Smith Theodore I.J., Ware Kent, Doroshov Serge I. (2008): Evidence of hexaploid karyotype in shortnose sturgeon. Genome, 51, 113-119
Gela D., Rodina M., Linhart O. (2008): The artificial reproduction of the sturgeons (Acipenser). Methodology edition (Technology Series). Research Institute of Fish Culture and Hydrobiology, University of South Bohemia in České Budějovice, Vodňany, 78, 24. (in Czech)
Glogowski J, Kolman R, Szczepkowski M, Horváth Á, Urbányi B, Sieczyński P, Rzemieniecki A, Domagała J, Demianowicz W, Kowalski R, Ciereszko A (2002): Fertilization rate of Siberian sturgeon (Acipenser baeri, Brandt) milt cryopreserved with methanol. Aquaculture, 211, 367-373
Grant P. R., Grant B. R. (1992): Hybridization of Bird Species. Science, 256, 193-197
Havelka M., Hulák M., Bailie D. A., Prodöhl P. A., Flajšhans M. (2013): Extensive genome duplications in sturgeons: new evidence from microsatellite data. Journal of Applied Ichthyology, 29, 704-708
Jarić I., Gessner J. (2012): Analysis of publications on sturgeon research between 1996 and 2010. Scientometrics, 90, 715-735
Jenneckens I., Meyer J.-N., Debus L., Pitra C., Ludwig A. (2000): Evidence of mitochondrial DNA clones of Siberian sturgeon, Acipenser baerii, within Russian sturgeon, Acipenser gueldenstaedtii, caught in the River Volga. Ecology Letters, 3, 503-508
King T.L., Lubinski B.A., Spidle A.P. (2001): Microsatellite DNA variation in Atlantic sturgeon (Acipenser oxyrinchus oxyrinchus) and cross-species amplification in the Acipenseridae. Conservation Genetics, 2, 103–119.
Koolboon Urai, Koonawootrittriron Skorn, Kamolrat Wongpathom, Na-Nakorn Uthairat (2014): Effects of parental strains and heterosis of the hybrid between Clarias macrocephalus and Clarias gariepinus. Aquaculture, 424-425, 131-139
Linhart Otomar, Rodina Marek, Cosson Jacky (2000): Cryopreservation of Sperm in Common Carp Cyprinus carpio: Sperm Motility and Hatching Success of Embryos. Cryobiology, 41, 241-250
Linhart Otomar, Rodina Marek, Flajshans Martin, Mavrodiev Nicola, Nebesarova Jana, Gela David, Kocour Martin (2006): Studies on sperm of diploid and triploid tench, Tinca tinca (L.). Aquaculture International, 14, 9-25
Liu Xiangjiang, Liang Hongwei, Li Zhong, Liang Yongjun, Lu Cuiyun, Li Chitao, Chang Yumei, Zou Guiwei, Hu Guangfu (2017): Performances of the hybrid between CyCa nucleocytplasmic hybrid fish and scattered mirror carp in different culture environments. Scientific Reports, 7, -
Ludwig Arne (2006): A sturgeon view on conservation genetics. European Journal of Wildlife Research, 52, 3-8
Ludwig Arne, Lippold Sebastian, Debus Lutz, Reinartz Ralf (2009): First evidence of hybridization between endangered sterlets (Acipenser ruthenus) and exotic Siberian sturgeons (Acipenser baerii) in the Danube River. Biological Invasions, 11, 753-760
Lugert Vincent, Thaller Georg, Tetens Jens, Schulz Carsten, Krieter Joachim (2016): A review on fish growth calculation: multiple functions in fish production and their specific application. Reviews in Aquaculture, 8, 30-42
MARKWITH S. H., STEWART D. J., DYER J. L. (2006): TETRASAT: a program for the population analysis of allotetraploid microsatellite data. Molecular Ecology Notes, 6, 586-589
Maury-Brachet Régine, Rochard Eric, Durrieu Gilles, Boudou Alain (2008): The ‘Storm of the Century’ (December 1999) and the accidental escape of Siberian sturgeons (Acipenser baerii) into the Gironde estuary (Southwest France). Environmental Science and Pollution Research, 15, 89-94
McQuown Eve C., Sloss Brian L., Sheehan Robert J., Rodzen Jeff, Tranah Gregory J., May Bernie (2000): Microsatellite Analysis of Genetic Variation in Sturgeon: New Primer Sequences for Scaphirhynchus and Acipenser. Transactions of the American Fisheries Society, 129, 1380-1388<1380:MAOGVI>2.0.CO;2
Memis D., Ercan E., Celikkale M.S., Timur M., Zarkua Z. (2009): Growth and survival rate of Russian sturgeon (Acipenser gueldenstaedtii) larvae from fertilized eggs to artificial feeding. Turkish Journal of Fisheries and Aquatic Sciences, 9, 47–52.
Nielsen Hanne Marie, Ødegård Jørgen, Olesen Ingrid, Gjerde Bjarne, Ardo Làszlò, Jeney Galina, Jeney Zsigmond (2010): Genetic analysis of common carp (Cyprinus carpio) strains. Aquaculture, 304, 14-21
Pikitch Ellen K, Doukakis Phaedra, Lauck Liz, Chakrabarty Prosanta, Erickson Daniel L (2005): Status, trends and management of sturgeon and paddlefish fisheries. Fish and Fisheries, 6, 233-265
Aminur Rah M., Arshad A., Marimuthu K., Ara R., Amin S.M.N. (2013): Inter-specific Hybridization and Its Potential for Aquaculture of Fin Fishes. Asian Journal of Animal and Veterinary Advances, 8, 139-153
Reddy P.V.G.K. (1999): Genetic resources of Indian major carps. FAO Fisheries Technical Paper No. 387.
Reinartz R., Lippold S., Lieckfeldt D., Ludwig A. (2011): Population genetic analyses of Acipenser ruthenus as a prerequisite for the conservation of the uppermost Danube population. Journal of Applied Ichthyology, 27, 477-483
Safronov A.S., Filipova O.P. (2000): Experiment on rearing the hybrid of Russian (Acipenser gueldenstaedti Br.) × Siberian (Acipenser baeri Br.) sturgeon in the warm water fish farm in the Vologda region. In: Book of Abstracts of the International Conference: Sturgeons on the Threshold of the XXIst Century. Astrakhan, USSR, 11–15. (in Russian)
Scheerer Paul D., Thorgaard Gary H. (1983): Increased Survival in Salmonid Hybrids by Induced Triploidy. Canadian Journal of Fisheries and Aquatic Sciences, 40, 2040-2044
Siegel S.N., Castellan J. (1988): Nonparametric Statistics for the Behavioral Sciences. McGraw-Hill, New York, USA.
Stech L., Linhart O., Shelton W.L., Mims S.D. (1999): Minimally invasive surgical removal of ovulated eggs of paddlefish (Polyodon spathula). Aquaculture International, 7, 129–133.
Szczepkowski M., Kolman R., Szcepkowska B. (2000): A comparison of selected morphometric characteristics of the juveniles of Siberian sturgeon (Acipenser baeri Brandt) and its hybrid with Russian sturgeon (Acipenser Gueldenstaedti Brandt). Archives of Polish Fisheries, 8, 193–204.
Vasilev V.P. (2009): Mechanisms of polyploid evolution in fish: Polyploidy in sturgeons. In: Carmona R., Domezain A., Gallego M.G., Hernando J.A., Rodríguez F., Ruiz-Rejón M. (eds): Biology, Conservation and Sustainable Development of Sturgeons. Springer, Dordrecht, the Netherlands, 97–117.
Wang J, Xia D (2002): Studies on fish heterosis with DNA fingerprinting. Aquaculture Research, 33, 942-947
Wei Q. W., Zou Y., Li P., Li L. (2011): Sturgeon aquaculture in China: progress, strategies and prospects assessed on the basis of nation-wide surveys (2007-2009). Journal of Applied Ichthyology, 27, 162-168
Welsh Amy B., Blumberg Marc, May Bernie (2003): Identification of microsatellite loci in lake sturgeon, Acipenser fulvescens, and their variability in green sturgeon, A. medirostris. Molecular Ecology Notes, 3, 47-55
Whitlock Michael C. (): Local drift load and the heterosis of interconnected populations. Heredity, 84, 452-
Zhang Xiaomin, Wu Wenhua, Li Linmiao, Ma Xufa, Chen Jinping (2013): Genetic variation and relationships of seven sturgeon species and ten interspecific hybrids. Genetics Selection Evolution, 45, 21-
download PDF

© 2019 Czech Academy of Agricultural Sciences