Effects of diet supplementation with herbal blend and sunflower seeds on fermentation parameters, microbial population, and fatty acid profile in rumen of sheep

https://doi.org/10.17221/17/2016-CJASCitation:Wencelová M., Váradyová Z., Pristaš P., Čobanová K., Plachá I., Kišidayová S. (2016): Effects of diet supplementation with herbal blend and sunflower seeds on fermentation parameters, microbial population, and fatty acid profile in rumen of sheep. Czech J. Anim. Sci., 61: 551-559.
download PDF
The study evaluated the rumen fermentation pattern, microbial population, fatty acid composition, and antioxidant status in the rumen fluid and blood of sheep in response to a diet supplemented with herbal blend (HB), sunflower seeds (SS), and a combination of both HB and SS (HBSS). The HB consisted of medicinal herbs typical for Central Europe. Four rumen fistulated rams were used in a 4 × 4 Latin square design and were fed a basal diet consisting of meadow hay (720 g dry matter (DM)/day) and barley grain (540 g DM/day) in the ratio of 60 : 40 and supplemented with no additive (Control), HB (10% replacement of meadow hay), SS (180 g DM/day), and HBSS (HB + SS). An increase in the molar proportion of iso-butyrate (P = 0.009) and iso-valerate (P = 0.034) was observed in the SS and HBSS diets as well as of ammonia N concentration in the HB, SS, and HBSS diets compared to control (P = 0.003). Concentrations of monounsaturated fatty acids of the HB, SS, and HBSS diets were increased (P = 0.041) compared to control, whereas concentrations of polyunsaturated fatty acids in the SS and HBSS diets were decreased (P = 0.023). Phagocytic activity in the blood was higher with all the diets vs control (P = 0.001). Qualitative evaluation of microbial population by denaturing gradient gel electrophoresis (DGGE) and principal component analysis (PCA) revealed no effects of dietary treatments on the composition of both eubacterial and ciliated protozoal populations. However, in spite of the supplementation of forage-concentrate diet by HB in the presence of SS, no negative effects on the fermentation patterns and rumen microbial population have been detected.
Alves Susana P., Santos-Silva José, Cabrita Ana R. J., Fonseca António J. M., Bessa Rui J. B., Schunck Wolf-Hagen (2013): Detailed Dimethylacetal and Fatty Acid Composition of Rumen Content from Lambs Fed Lucerne or Concentrate Supplemented with Soybean Oil. PLoS ONE, 8, e58386-  https://doi.org/10.1371/journal.pone.0058386
AOAC (1990): Official Methods of Analysis of AOAC International. 15th Ed. Association of Official Analytical Chemists, Arlington, USA.
Beauchemin K.A., McGinn S.M., Benchaar C., Holtshausen L. (2009): Crushed sunflower, flax, or canola seeds in lactating dairy cow diets: Effects on methane production, rumen fermentation, and milk production. Journal of Dairy Science, 92, 2118-2127  https://doi.org/10.3168/jds.2008-1903
Belanche A., de la Fuente G., Newbold C. J. (): Effect of progressive inoculation of fauna-free sheep with holotrich protozoa and total-fauna on rumen fermentation, microbial diversity and methane emissions. FEMS Microbiology Ecology, 91, fiu026-fiu026  https://doi.org/10.1093/femsec/fiu026
Benzie Iris F.F., Strain J.J. (1996): The Ferric Reducing Ability of Plasma (FRAP) as a Measure of “Antioxidant Power”: The FRAP Assay. Analytical Biochemistry, 239, 70-76  https://doi.org/10.1006/abio.1996.0292
Bhatta R., Baruah L., Saravanan M., Suresh K. P., Sampath K. T. (2013): Effect of medicinal and aromatic plants on rumen fermentation, protozoa population and methanogenesis in vitro. Journal of Animal Physiology and Animal Nutrition, 97, 446-456  https://doi.org/10.1111/j.1439-0396.2012.01285.x
Bligh E. G., Dyer W. J. (1959): A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION. Canadian Journal of Biochemistry and Physiology, 37, 911-917  https://doi.org/10.1139/o59-099
Broderick G.A., Kang J.H. (1980): Automated Simultaneous Determination of Ammonia and Total Amino Acids in Ruminal Fluid and In Vitro Media. Journal of Dairy Science, 63, 64-75  https://doi.org/10.3168/jds.S0022-0302(80)82888-8
Chen G., Russell J.B. (1989): More monensin-sensitive, ammonia-producing bacteria from the rumen. Applied and Environmental Microbiology, 55, 1052–1057.
Durmic Z., McSweeney C.S., Kemp G.W., Hutton P., Wallace R.J., Vercoe P.E. (2008): Australian plants with potential to inhibit bacteria and processes involved in ruminal biohydrogenation of fatty acids. Animal Feed Science and Technology, 145, 271-284  https://doi.org/10.1016/j.anifeedsci.2007.05.052
Eschenlauer S. C. P., McKain N., Walker N. D., McEwan N. R., Newbold C. J., Wallace R. J. (2002): Ammonia Production by Ruminal Microorganisms and Enumeration, Isolation, and Characterization of Bacteria Capable of Growth on Peptides and Amino Acids from the Sheep Rumen. Applied and Environmental Microbiology, 68, 4925-4931  https://doi.org/10.1128/AEM.68.10.4925-4931.2002
Gladine Cécile, Rock E., Morand C., Bauchart D., Durand D. (2007): Bioavailability and antioxidant capacity of plant extracts rich in polyphenols, given as a single acute dose, in sheep made highly susceptible to lipoperoxidation. British Journal of Nutrition, 98, -  https://doi.org/10.1017/S0007114507742666
Glasser F., Ferlay A., Chilliard Y. (2008): Oilseed Lipid Supplements and Fatty Acid Composition of Cow Milk: A Meta-Analysis. Journal of Dairy Science, 91, 4687-4703  https://doi.org/10.3168/jds.2008-0987
Ivan M., Mir P. S., Mir Z., Entz T., He M. L., McAllister T. A. (2004): Effects of dietary sunflower seeds on rumen protozoa and growth of lambs. British Journal of Nutrition, 92, 303-  https://doi.org/10.1079/BJN20041178
Jalc D., Ceresnakova Z. (2002): Effect of plant oils and malate on rumen fermentation in vitro. Czech Journal of Animal Science, 47, 106–111.
Jenkins T.C. (1993): Lipid Metabolism in the Rumen. Journal of Dairy Science, 76, 3851-3863  https://doi.org/10.3168/jds.S0022-0302(93)77727-9
Jo C., Ahn D. U. (1998): Fluorometric analysis of 2-thiobarbituric acid reactive substances in turkey. Poultry Science, 77, 475-480  https://doi.org/10.1093/ps/77.3.475
Karásková K., Suchý P., Straková E. (): Current use of phytogenic feed additives in animal nutrition: a review. Czech Journal of Animal Science, 60, 521-530  https://doi.org/10.17221/8594-CJAS
Kresanek Jr. J., Kresanek J. (2008): Atlas of Medicinal Plants and Berries. Osveta, Bratislava, Slovak Republic.
Makkar Harinder P S, Blümmel Michael, Borowy Norbert K, Becker Klaus (1993): Gravimetric determination of tannins and their correlations with chemical and protein precipitation methods. Journal of the Science of Food and Agriculture, 61, 161-165  https://doi.org/10.1002/jsfa.2740610205
Mandal G.P., Roy A., Patra A.K. (2014): Effects of feeding plant additives rich in saponins and essential oils on the performance, carcass traits and conjugated linoleic acid concentrations in muscle and adipose tissues of Black Bengal goats. Animal Feed Science and Technology, 197, 76-84  https://doi.org/10.1016/j.anifeedsci.2014.08.008
Nübel U, Engelen B, Felske A, Snaidr J, Wieshuber A, Amann R I, Ludwig W, Backhaus H (1996): Sequence heterogeneities of genes encoding 16S rRNAs in Paenibacillus polymyxa detected by temperature gradient gel electrophoresis.. Journal of Bacteriology, 178, 5636-5643  https://doi.org/10.1128/jb.178.19.5636-5643.1996
Paglia D.E., Valentine W.N. (1967): Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. Journal of Laboratory and Clinical Medicine, 70, 158–169.
Patra A.K., Yu Z. (2013): Effects of coconut and fish oils on ruminal methanogenesis, fermentation, and abundance and diversity of microbial populations in vitro. Journal of Dairy Science, 96, 1782-1792  https://doi.org/10.3168/jds.2012-6159
Tripathi M.K. (2014): Effect of nutrition on production, composition, fatty acids and nutraceutical properties of milk. Journal of Advances in Dairy Research, 2, 115.
Van Soest P.J., Robertson J.B., Lewis B.A. (1991): Methods for Dietary Fiber, Neutral Detergent Fiber, and Nonstarch Polysaccharides in Relation to Animal Nutrition. Journal of Dairy Science, 74, 3583-3597  https://doi.org/10.3168/jds.S0022-0302(91)78551-2
Vasta V., Yanez-Ruiz D. R., Mele M., Serra A., Luciano G., Lanza M., Biondi L., Priolo A. (): Bacterial and Protozoal Communities and Fatty Acid Profile in the Rumen of Sheep Fed a Diet Containing Added Tannins. Applied and Environmental Microbiology, 76, 2549-2555  https://doi.org/10.1128/AEM.02583-09
Weisburg W G, Barns S M, Pelletier D A, Lane D J (1991): 16S ribosomal DNA amplification for phylogenetic study.. Journal of Bacteriology, 173, 697-703  https://doi.org/10.1128/jb.173.2.697-703.1991
Wencelová M., Váradyová Z., Mihaliková K., Čobanová K., Plachá I., Pristaš P., Jalč D., Kišidayová S. (2015): Rumen fermentation pattern, lipid metabolism and the microbial community of sheep fed a high-concentrate diet supplemented with a mix of medicinal plants. Small Ruminant Research, 125, 64-72  https://doi.org/10.1016/j.smallrumres.2015.01.028
Williams A.G., Coleman G.S. (1992): The Rumen Protozoa. Springer-Verlag Inc., New York, USA.
Zheng Wei, Wang Shiow Y. (2001): Antioxidant Activity and Phenolic Compounds in Selected Herbs. Journal of Agricultural and Food Chemistry, 49, 5165-5170  https://doi.org/10.1021/jf010697n
download PDF

© 2022 Czech Academy of Agricultural Sciences | Prohlášení o přístupnosti