Effect of extender on the quality and incubation resilience of cryopreserved Holstein bull semen

https://doi.org/10.17221/196/2021-CJASCitation:

Pytlík J., Savvulidi F.G., Ducháček J., Codl R., Vrhel M., Nagy S., Stádník L. (2022): Effect of extender on the quality and incubation resilience of cryopreserved Holstein bull semen. Czech J. Anim. Sci., 67: 75–86.

download PDF

There are still concerns over sperm quality when plant-based diluents are used instead of animal-based semen extenders. Therefore, in our study we compared the effects of one soybean lecithin-based (AndroMed®) and two egg yolk-based (BullXcell® and Optidyl®) commercially available extenders on post-thaw in vitro sperm quality. Fifty ejaculates collected from ten bulls were aliquoted into three parts and diluted with the above-mentioned extenders. Post-thaw sperm viability, mitochondrial membrane potential (MMP), plasma membrane integrity, and acrosome status were analysed immediately after thawing (0 h) and at an hourly interval during 2-h incubation at 38 °C. Sperm functionality variables were assessed by simultaneous quadruple staining using flow cytometry. Semen stored in Optidyl® had a greater viability, plasma membrane and acrosome integrity than that stored in AndroMed® and BullXcell® (P < 0.05). With the use of BullXcell® there was a higher percentage of sperm with high MMP (P < 0.05) when compared with the other extenders. The incubation affected the development of sperm quality parameters differently as the variables related to the plasma membrane showed an increase, while MMP and acrosome integrity showed a decline. Although the semen from all bulls responded to treatments in a similar manner, significant intra- and inter-male differences were found. In conclusion, the results clearly displayed the beneficial effects of egg yolk over soybean lecithin supplementation on in vitro sperm quality.

References:
Aires VA, Hinsch KD, Mueller-Schloesser F, Bogner K, Mueller-Schloesser S, Hinsch E. In vitro and in vivo comparison of egg yolk-based and soybean lecithin-based extenders for cryopreservation of bovine semen. Theriogenology. 2003 Jul 1;60(2):269-79. https://doi.org/10.1016/S0093-691X(02)01369-9
 
Ansari MS, Rakha BA, Malik MF, Andrabi SMH, Ullah N, Iqbal R, Holt WV, Akhter S. Effect of cysteine addition to the freezing extender on the progressive motility, viability, plasma membrane and DNA integrity of Nili-Ravi buffalo (Bubalus bubalis) bull spermatozoa. J Appl Anim Res. 2014 Dec 20;44(1):36-41. https://doi.org/10.1080/09712119.2014.987292
 
Anzar M, Kroetsch T, Boswall L. Cryopreservation of bull semen shipped overnight and its effect on post-thaw sperm motility, plasma membrane integrity, mitochondrial membrane potential and normal acrosomes. Anim Reprod Sci. 2011 Jun 1;126(1-2):23-31. https://doi.org/10.1016/j.anireprosci.2011.04.018
 
Beran J, Stadnik L, Bezdicek J, Louda F, Citek J, Duchacek J. Effect of sire and extender on sperm motility and share of live or dead sperm in bulls’ fresh ejaculate and in AI doses after thawing. Arch Anim Breed. 2012 Oct 10;55(3):207-18. https://doi.org/10.5194/aab-55-207-2012
 
Bucher K, Malama E, Siuda M, Janett F, Bollwein H. Multicolor flow cytometric analysis of cryopreserved bovine sperm: A tool for the evaluation of bull fertility. J Dairy Sci. 2019 Dec 1;102(12):11652-69. https://doi.org/10.3168/jds.2019-16572
 
Casas I, Sancho S, Briz M, Pinart E, Bussalleu E, Yeste M, Bonet S. Freezability prediction of boar ejaculates assessed by functional sperm parameters and sperm proteins. Theriogenology. 2009 Oct 15;72(7):930-48. https://doi.org/10.1016/j.theriogenology.2009.07.001
 
Crespilho AM, Nichi M, Guasti PN, Freitas-Dell’Aqua CP, Sa Filho MF, Maziero RR, Dell’Aqua JA Jr, Papa FO. Sperm fertility and viability following 48 h of refrigeration: Evaluation of different extenders for the preservation of bull semen in liquid state. Anim Reprod Sci. 2014 May 1;146(3-4):126-33. https://doi.org/10.1016/j.anireprosci.2014.02.020
 
Dolezalova M, Stadnik L, Biniova Z, Duchacek J, Beran J. Effect of freezing curve type on bull spermatozoa motility after thawing. Acta Vet Brno. 2015 Dec 19;84(4):383-91. https://doi.org/10.2754/avb201584040383
 
Fleisch A, Malama E, Witschi U, Leiding C, Siuda M, Janett F, Bollwein H. Effects of an extension of the equilibration period up to 96 hours on the characteristics of cryopreserved bull semen. Theriogenology. 2017 Feb 1;89:255-62. https://doi.org/10.1016/j.theriogenology.2016.10.018
 
Ghareeb S, Haron W, Yusoff R, Yimer N, Baiee F, Ahmedeltayeb T, Ebrahimi M. Post-thaw evaluation of cryopreserved bull semen extended in four different semen extenders. Aust J Basic Appl Sci. 2017 Apr;11(5):80-7.
 
Gurler H, Malama E, Heppelmann M, Calisici O, Leiding C, Kastelic JO, Bollwein H. Effects of cryopreservation on sperm viability, synthesis of reactive oxygen species and DNA damage of bovine sperm. Theriogenology. 2016 Jul 15;86(2):562-71. https://doi.org/10.1016/j.theriogenology.2016.02.007
 
Hashem NM, Gonzalez-Bulnes A. State-of-the-art and prospective of nanotechnologies for smart reproductive management of farm animals. Animals. 2020 May 13;10(5): 14 p. https://doi.org/10.3390/ani10050840
 
Januskauskas A, Gil J, Soderquist L, Haard MGM, Haard MC, Johannisson A, Rodriguez-Martinez H. Effect of cooling rates on post-thaw sperm motility, membrane integrity, capacitation status and fertility of dairy bull semen used for artificial insemination in Sweden. Theriogenology. 1999 Sep 1;52(4):641-58. https://doi.org/10.1016/S0093-691X(99)00159-4
 
Khalil WA, El-Harairy MA, Zeidan AEB, Hassan MAE, Mohey-Elsaeed O. Evaluation of bull spermatozoa during and after cryopreservation: Structural and ultrastructural insights. Int J Vet Sci Med. 2018 Jan 1;6(1):S49-56. https://doi.org/10.1016/j.ijvsm.2017.11.001
 
Kumar P, Saini M, Kumar D, Balhara AK, Yadav SP, Singh P, Yadav PS. Liposome-based semen is suitable alternative to egg yolk-based extender for cryopreservation of buffalo (Bubalus bubalis) semen. Anim Reprod Sci. 2015 Aug 1;159:38-45. https://doi.org/10.1016/j.anireprosci.2015.05.010
 
Layek SS, Mohanty TK, Kumaresan A, Parks JE. Cryopreservation of bull semen: Evolution from egg yolk based to soybean based extenders. Anim Reprod Sci. 2016 Sep 1;172:1-9. https://doi.org/10.1016/j.anireprosci.2016.04.013
 
Leite TG, do Vale Filho VR, de Arruda RP, de Andrade AFC, Emerick LL, Zaffalon FG, Martins JAM, de Andrade VJ. Effects of extender and equilibration time on post-thaw motility and membrane integrity of cryopreserved Gyr bull semen evaluated by CASA and flow cytometry. Anim Reprod Sci. 2010 Jul 1;120(1-4):31-8. https://doi.org/10.1016/j.anireprosci.2010.04.005
 
Mata-Campuzano M, Alvarez-Rodriguez M, Alvarez M, Tamayo-Canul J, Anel L, de Paz P, Martinez-Pastor F. Post-thawing quality and incubation resilience of cryopreserved ram spermatozoa are affected by antioxidant supplementation and choice of extender. Theriogenology. 2015 Mar 1;83(4):520-8. https://doi.org/10.1016/j.theriogenology.2014.10.018
 
Miguel-Jimenez S, Montserrat Rivera del Alamo M, Alvare-Rodriguez M, Hidalgo CO, Pena AI, Muino R, Rodriguez-Gil JE, Mogas T. In vitro assessment of egg yolk-, soya bean lecithin- and liposome-based extenders for cryopreservation of dairy bull semen. Anim Reprod Sci. 2020 Apr 1;215:106315. https://doi.org/10.1016/j.anireprosci.2020.106315
 
Morrell J, Valeanu A, Lundeheim N, Johannisson A. Sperm quality in frozen beef and dairy bull semen. Acta Vet Scand. 2018 Jul 4;60: 10 p. https://doi.org/10.1186/s13028-018-0396-2
 
Muino R, Fernandez M, Pena AI. Post-thaw survival and longevity of bull spermatozoa frozen with an egg yolk-based or two egg yolk-free extenders after an equilibration period of 18 h. Reprod Domest Anim. 2007 Jun;42(3):305-11. https://doi.org/10.1111/j.1439-0531.2006.00784.x
 
Murphy EM, O’Meara C, Eivers B, Lonergan P, Fair S. Comparison of plant- and egg yolk-based semen diluents on in vitro sperm kinematics and in vivo fertility of frozen-thawed bull semen. Anim Reprod Sci. 2018 Apr 1;191:70-5. https://doi.org/10.1016/j.anireprosci.2018.02.010
 
Rastegarnia A, Shahverdi A, Topraggaleah TR, Shafiepour V. In vitro comparison of soybean lecithin-based extenders for cryopreservation of buffalo (Bubalus bubalis) semen. Comp Clin Pathol. 2014 Jul;23(4):893-900. https://doi.org/10.1007/s00580-013-1708-6
 
Savvulidi FG, Ptacek M, Malkova A, Beranek J, Stadnik L. Optimizing the conventional method of sperm freezing in liquid nitrogen vapour for Wallachian sheep conservation program. Czech J Anim Sci. 2021 Feb 15;66(2):55-64. https://doi.org/10.17221/226/2020-CJAS
 
Sellem E, Broekhuijse MLWJ, Chevrier L, Camugli S, Schmitt E, Schibler L, Koenen EPC. Use of combinations of in vitro quality assessments to predict fertility of bovine semen. Theriogenology. 2015 Jul 30;84(9):1447-54. https://doi.org/10.1016/j.theriogenology.2015.07.035
 
Sieme H, Oldenhof H, Wolkers WF. Sperm membrane behaviour during cooling and cryopreservation. Reprod Dom Anim. 2015 Sep;50(3):20-6. https://doi.org/10.1111/rda.12594
 
Singh AK, Kumar A, Honparkhe M, Kaur S, Kaur H, Ghuman SPS, Brar PS. Comparison of in vitro and in vivo fertilizing potential of buffalo bull semen frozen in egg yolk-, soya bean lecithin-, and liposome-based extenders. Reprod Domest Anim. 2018 Feb;53(1):195-202. https://doi.org/10.1111/rda.13092
 
Ugur MR, Abdelrahman AS, Evans HC, Gilmore AA, Hitit M, Arifiantini RI, Purwantara B, Kaya A, Memili E. Advances in cryopreservation of bull sperm. Front Vet Sci. 2019 Aug 27;6: 15 p. https://doi.org/10.3389/fvets.2019.00268
 
Varela E, Rojas M, Restrepo G. Membrane stability and mitochondrial activity of bovine sperm frozen with low-density lipoproteins and trehalose. Reprod Domest Anim. 2020 Feb;55(2):146-53. https://doi.org/10.1111/rda.13599
 
Zorova LD, Popkov VA, Plotnikov EY, Silachev DN, Pevzner IB, Jankauskas SS, Babenko VA, Zorov SD, Balakireva AV, Juhazsova M, Sollot SJ, Zorov DB. Mitochondrial membrane potential. Anal Biochem. 2018 Jul 1;552:50-9. https://doi.org/10.1016/j.ab.2017.07.009
 
download PDF

© 2022 Czech Academy of Agricultural Sciences | Prohlášení o přístupnosti