Effects of different stripping methods of female and activation medium on fertilization success in northern pike (Esox lucius)

https://doi.org/10.17221/19/2016-CJASCitation:Cejko B.I., Sarosiek B., Krejszeff S., Judycka S., Szczepkowski M., Szczepkowska B., Kowalski R.K. (2016): Effects of different stripping methods of female and activation medium on fertilization success in northern pike (Esox lucius). Czech J. Anim. Sci., 61: 481-486.
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In this study, the quality of northern pike eggs collected by the traditional method (hand stripping) and the pneumatic method (air stripping) was compared. Additionally, different activation solutions (Billard solution, hatchery water, and Woynarovich solution) were tested for their effects on egg fertilization under artificial conditions. After the eggs were collected, the Pseudo-Gonado-Somatic Index (PGSI) was measured. Although the values of the PGSI in the samples obtained with use of air stripping were lower (13.8 ± 3.9%), they did not differ statistically from those obtained by hand stripping (16.5 ± 5.4%). Hatchery water and Woynarovich solution were found to be the most suitable solutions for sperm activation using the Computer-Assisted Sperm Analysis (CASA) system as compared to the Billard solution. Hand stripping was found to negatively affect the percentage of fertilized eggs and the percentage of hatched larvae in samples fertilized in hatchery water and Woynarovich solution. When the traditional method of egg collection was used, there were no differences in the percentage of fertilization and the percentage of hatched larvae between Billard solution (54.0 ± 21.5% and 44.1 ± 21.9%, respectively), hatchery water (60.0 ± 22.5% and 55.9 ± 22.8%, respectively), and Woynarovich solution (72.0 ± 25.8% and 69.0 ± 23.9%, respectively) treatments. Air stripped eggs showed a higher fertilization rate when hatchery water or Woynarovich solution was applied (86.2 ± 9.3% and 92.4 ± 3.9%, respectively). Also hatching rate was the highest in these samples (83.0 ± 8.4% and 88.3 ± 6.2%). The application of the pneumatic method and Woynarovich solution to northern pike artificial fertilization resulted in higher fertilization and hatching rates as compared to other techniques. Because this was successful in northern pike, the use of air stripping is a promising option for artificial reproduction in other fish species.
Hadi Alavi S.M., Rodina Marek, Viveiros Ana T.M., Cosson Jacky, Gela David, Boryshpolets Sergei, Linhart Otomar (2009): Effects of osmolality on sperm morphology, motility and flagellar wave parameters in Northern pike (Esox lucius L.). Theriogenology, 72, 32-43  https://doi.org/10.1016/j.theriogenology.2009.01.015
Billard R., Marcel Jacqueline (1980): Stimulation of spermiation and induction of ovulation in pike (Esox lucius). Aquaculture, 21, 181-195  https://doi.org/10.1016/0044-8486(80)90027-7
Dietrich Grzegorz J., Wojtczak Mariola, Słowińska Mariola, Dobosz Stefan, Kuźmiński Henryk, Ciereszko Andrzej (2007): Broken eggs decrease pH of rainbow trout (Oncorhynchus mykiss) ovarian fluid. Aquaculture, 273, 748-751  https://doi.org/10.1016/j.aquaculture.2007.07.013
Gage M (2004): Spermatozoal Traits and Sperm Competition in Atlantic Salmon Relative Sperm Velocity Is the Primary Determinant of Fertilization Success. Current Biology, 14, 44-47  https://doi.org/10.1016/S0960-9822(03)00939-4
Kobayashi Toru, Fushiki Shozo, Ueno Koichi (2004): Improvement of Sperm Motility of Sex-Reversed Male Rainbow Trout, Oncorhynchus mykiss, by Incubation in High-pH Artificial Seminal Plasma. Environmental Biology of Fishes, 69, 419-425  https://doi.org/10.1023/B:EBFI.0000022904.35065.e8
Lahnsteiner F, Weismann T, Patzner R A (1998): An efficient method for cryopreservation of testicular sperm from the Northern pike, Esox lucius L.. Aquaculture Research, 29, 341-347  https://doi.org/10.1111/j.1365-2109.1998.tb01139.x
Szabo Tamas (2003): Ovulation induction in northern pike Esox lucius L. using different GnRH analogues, Ovaprim, Dagin and carp pituitary. Aquaculture Research, 34, 479-486  https://doi.org/10.1046/j.1365-2109.2003.00835.x
Wojtczak Mariola, Dietrich Grzegorz J., Słowińska Mariola, Dobosz Stefan, Kuźmiński Henryk, Ciereszko Andrzej (2007): Ovarian fluid pH enhances motility parameters of rainbow trout (Oncorhynchus mykiss) spermatozoa. Aquaculture, 270, 259-264  https://doi.org/10.1016/j.aquaculture.2007.03.010
Woynarovich E., Woynarovich A. (1980): Modified technology for elimination of stickiness of common carp Cyprinus carpio eggs. Aquacultura Hungarica, 2, 19–21.
Żarski D., Horváth Á., Kotrik L., Targońska K., Palińska K., Krejszeff S., Bokor Z., Urbányi B., Kucharczyk D. (2012): Effect of different activating solutions on the fertilization ability of Eurasian perch, Perca fluviatilis  L., eggs. Journal of Applied Ichthyology, 28, 967-972  https://doi.org/10.1111/jai.12098
Zhang J. J., Li S. Z., Tulake K., Yan Q. P., Li W. J. (2011): The effects of extenders and sperm-egg ratios on fertilizing ability of cryopreserved testicular sperm of northern pike (Esox lucius L.). Journal of Applied Ichthyology, 27, 1037-1040  https://doi.org/10.1111/j.1439-0426.2010.01586.x
Zurbuch Peter E. (1965): A Structure for Easy Fish Recovery during Drainage of an Impoundment. The Progressive Fish-Culturist, 27, 237-238  https://doi.org/10.1577/1548-8640(1965)27[237:ASFEFR]2.0.CO;2
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