Molecular characterization and A1/A2 genotyping of casein beta gene in zebu and crossbred cattle of Bangladesh

https://doi.org/10.17221/206/2021-CJASCitation:

Pabitra M.H., Maruf T.M., Mony S.I., Ebnat R., Hoque M.R., Bhuiyan M.S.A. (2022): Molecular characterization and A1/A2 genotyping of casein beta gene in zebu and crossbred cattle of Bangladesh. Czech J. Anim. Sci., 67: 318–326.

supplementary materialdownload PDF

Casein beta (CSN2) is the most explored gene in cattle due to its potential impact on human health. Here, we investigated the entire coding sequence of CSN2 gene except the last two codons for detection of polymorphisms in different cattle populations of Bangladesh as well as genotyping of A1 and A2 allelic variants using a newly developed allele specific polymerase chain reaction (AS-PCR) based the genotyping protocol. Five primer pairs were used to amplify the coding sequences of CSN2 gene. This study included a total of 258 DNA samples from six Bangladeshi zebu populations and one Holstein Friesian (HF) derived crossbred cattle population. Sequence analysis detected five nonsynonymous mutations in the coding sequence of CSN2 gene that defined five allelic variants as A1, A2, B, F and I. In addition, substitution of GTA (Val) by GCA (Ala) at position 197 resulted in an undefined allele in the zebu cattle population of Bangladesh that has not yet been reported elsewhere. Like other Bos indicus cattle populations, A2 allele is predominant in the studied zebu populations. The mean frequencies of A1A1, A1A2 and A2A2 genotypes were 0.02, 0.16 and 0.82, respectively, in zebu cattle populations while the corresponding allele frequencies were A1 (0.10) and A2 (0.90). In opposite, the aforesaid genotype frequencies were 0.14 (A1A1), 0.50 (A1A2) and 0.36 (A2A2) in the HF crossbred population with allele frequencies of 0.39 (A1) and 0.61 (A2). The adopted AS-PCR method was found cost-effective, rapid and had high specificity for genotyping of A1 and A2 allelic variants. Altogether, this study provides information for the selection of desired zebu and crossbred individuals in order to produce premium quality milk as well as to design a breeding plan in the crossbreeding program.

References:
Ayaz, Suhail SM, Ahmad I, Zeb MT, Khan R, Ijaz A, Ahmad I, Riaz MH, Ali F, Khan K, Khan A. Detection of A2A2 genotype of beta casein protein (CSN2) gene in local, exotic and crossbred cattle in Pakistan. Anim Biotechnol. 2022 Mar 2;4: 12 p.  https://doi.org/10.1080/10495398.2022.2031204
 
Bell SJ, Grochoski GT, Clarke AJ. Health implications of milk containing beta-casein with the A2 genetic variant. Crit Rev Food Sci Nutr. 2006 Jan 1;46(1):93-100.
 
Bhuiyan AKFH, Rashid MM, Khan RA, Habib MA, Bhuiyan MSA, Faiz MA. Progeny tested bull production for dairy cattle development in Bangladesh. Bang J Anim Sci. 2015 Dec 19;44(2):106-12. https://doi.org/10.3329/bjas.v44i2.26010
 
Boettcher PJ, Caroli A, Stella A, Chessa S, Budelli E, Canavesi F, Ghiroldi S, Pagnacco G. Effects of casein haplotypes on milk production traits in Italian Holstein and Brown Swiss cattle. J Dairy Sci. 2004 Dec 1;87(12):4311-7. https://doi.org/10.3168/jds.S0022-0302(04)73576-6
 
Bonsing J, Ring JM, Stewart AF, Mackinlay AG. Complete nucleotide sequence of the bovine β-casein gene. Aust J Biol Sci. 1988;41(4):527-37. https://doi.org/10.1071/BI9880527
 
Caroli AM, Chessa S, Erhardt GJ. Invited review: Milk protein polymorphisms in cattle: Effect on animal breeding and human nutrition. J Dairy Sci. 2009 Nov 1;92(11):5335-52. https://doi.org/10.3168/jds.2009-2461
 
Chessa S, Bulgari O, Rossoni A, Ceriotti G, Caroli A. Bovine β-casein: Detection of two single nucleotide polymorphisms by bidirectional allele specific polymerase chain reaction (BAS-PCR) and monitoring of their variation. Open J Anim Sci. 2013 Jan;3(1):36-41. https://doi.org/10.4236/ojas.2013.31005
 
Dai R, Fang Y, Zhao W, Liu S, Ding J, Xu K, Yang L, He C, Ding F, Meng H. Identification of alleles and genotypes of beta-casein with DNA sequencing analysis in Chinese Holstein cow. J Dairy Res. 2016 Aug;83(3):312-6. https://doi.org/10.1017/S0022029916000303
 
Falconer DS, Mackay TFC. Introduction to quantitative genetics. 4th ed. Harlow: Addison Wesley Longman; 1996. p. 1-20.
 
Farrell HM, Jimenez-Flores R, Bleck GT, Brown EM, Butler JE, Creamer LK, Hicks CL, Hollar CM, Ng-Kwai-Hang KF, Swaisgood HE. Nomenclature of the proteins of cows’ milk – Sixth revision. J Dairy Sci. 2004 Jun 1;87(6):1641-74. https://doi.org/10.3168/jds.S0022-0302(04)73319-6
 
Ganguly I, Kumar S, Gaur GK, Singh U, Kumar A, Kumar S, Mann S, Sharma A. Status of β-casein (CSN2) polymorphism in Frieswal (HF X Sahiwal crossbred) cattle. Int J Biotec Bioeng Res. 2013 Apr;4(3):249-56.
 
Hall TA. BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser. 1999 Jan 1;41(41):95-8.
 
Hamid MA, Rahman A, Zaman MA, Hossain KM. Cattle genetic resources and their conservation in Bangladesh. Asian J Anim Sci. 2017 Feb;11(2):54-64.
 
Hanusova E, Huba J, Oravcova M, Polak P, Vrtkova I. Genetic variants of beta-casein in Holstein dairy cattle in Slovakia. Slovak J Anim Sci. 2010 Apr;43(2):63-6.
 
Kaminski S, Cieslinska A, Kostyra E. Polymorphism of bovine beta-casein and its potential effect on human health. J Appl Genet. 2007 Sep;48(3):189-98.
 
Kumar S, Stecher G, Tamura K. MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Bio Evol. 2016 Jul;33(7):1870-4. https://doi.org/10.1093/molbev/msw054
 
Kumar S, Singh RV, Chauhan A. Molecular characterization of A1/A2 beta-casein alleles in Vrindavani crossbred and Sahiwal cattle. Ind J Anim Res. 2019 Feb 1;53(2):151-5. https://doi.org/10.18805/ijar.B-3493
 
Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, McWilliam H, Valentin F, Wallace IM, Wilm A, Lopez R. Clustal W and Clustal X version 2.0. Bioinformatics. 2007 Nov 1;23(21):2947-8.
 
Malarmathi M, Senthilkumar TMA, Parthiban M, Muthuramalingam T, Palanisammi A. Analysis of ß-casein gene for A1 and A2 genotype using allele specific PCR in Kangeyam and Holstein-Friesian crossbred cattle in Tamil Nadu. Ind J Vet Anim Sci Res. 2014 Jul 4;43(4):310-5.
 
Massella E, Piva S, Giacometti F, Liuzzo G, Zambrini AV, Serraino A. Evaluation of bovine beta casein polymorphism in two dairy farms located in northern Italy. Ital J Food Safety. 2017 Aug 16;6(3): 3 p. https://doi.org/10.4081/ijfs.2017.6904
 
McLachlan CN. β-casein A1, ischaemic heart disease mortality and other illnesses. Med Hypotheses. 2001 Feb 1;56(2):262-72. https://doi.org/10.1054/mehy.2000.1265
 
Meier S, Korkuc P, Arends D, Brockmann GA. DNA Sequence variants and protein haplotypes of casein genes in German Black Pied cattle (DSN). Front Genet. 2019 Nov 8;10: 9 p.
 
Miluchova M, Gabor M, Trakovicka A. Analysis of beta-casein gene (CSN2) polymorphism in different breeds of cattle. Scient Pap Anim Sci Biotecnol. 2014 Dec 1;47(2):56-9.
 
Mishra BP, Mukesh M, Prakash B, Sodhi M, Kapila R, Kishore A, Kataria RS, Joshi BK, Rasool TJ, Bujarbaruah KM. Status of milk protein, β-casein variants among Indian milch animals. Ind J Anim Sci. 2009 Jul;79(7):722-5.
 
Muhammed EM, Stephen M. ß-casein A1A2 polymorphism and milk yield in Vechur, Kasargode dwarf and Crossbred cattle. J Indian Vet Assoc. 2012 Mar;10(3):5-9.
 
Olenski K, Kaminski S, Szyda J, Cieslinska A. Polymorphism of the beta-casein gene and its associations with breeding value for production traits of Holstein-Friesian bulls. Livest Sci. 2010 Jun 1;131(1):137-40. https://doi.org/10.1016/j.livsci.2010.02.023
 
Patel AC, Patel C, Zala H, Sheikh Y, Dadheech T, Mankad P, Shah SV, Rank DN. Genotyping of indigenous cattle breeds and their exotic crosses for β-casein milk type using PCR-RFLP and sequencing. Int J Livest Res. 2019 May;9(5):249-59.  https://doi.org/10.5455/ijlr.20190216104000
 
Ramesha KP, Rao A, Basavaraju M, Alex R, Kataktalware MA, Jeyakumar S, Varalakshmi S. Genetic variants of β-casein in cattle and buffalo breeding bulls in Karnataka state of India. Indian J Biotecnol. 2016 Apr;15(2):178-81.
 
Rijnkels M. Multispecies comparison of the casein gene loci and evolution of casein gene family. J Mammary Gland Biol Neoplasia. 2002 Jul;7(3):327-45. https://doi.org/10.1023/A:1022808918013
 
Saran M, Gurao A, Joshi RK, Kashyap SK. Prevalence of the beta-casein variants among Tharparkar, Rathi, Sahiwal, Kankrej and Crossbred and its influence under selective pressure. Int J Curr Microbiol Appl Sci. 2019;8(3):1842-8. https://doi.org/10.20546/ijcmas.2019.803.218
 
Sodhi M, Kataria RS, Niranjan SK, Parvesh K, Verma P, Swami SK, Sharma A, Bharti VK, Kumar B, Iqbal M, Rabgais S, Kumar P, Giri A, Kalia S, Gagoi D, Sarangi PP, Mukesh M. Sequence characterisation and genotyping of allelic variants of beta-casein gene establishes native cattle of Ladakh to be a natural resource for A2 milk. Defence Life Sci J. 2018 Apr;3(2):177-81. https://doi.org/10.14429/dlsj.3.12574
 
supplementary materialdownload PDF

© 2022 Czech Academy of Agricultural Sciences | Prohlášení o přístupnosti