Copper bioavailability, mineral utilization, and lipid metabolism in broilers A., Dai S., Wu X., Cai Z. (2019): Copper bioavailability, mineral utilization, and lipid metabolism in broilers. Czech J. Anim. Sci., 64: 483-490.
download PDF

The study was conducted to investigate the effects of copper (Cu) sources and levels on mineral utilization, tissue copper residues, and lipid metabolism in Arbor Acres broilers. A total of 640 male broilers were randomly divided into 5 groups with 8 replicates per group and 16 broilers per replicate. The experiment was used in a 2 × 2 + 1 factorial experiment design. Broilers in the control group were fed a basal diet, and animals in the other four groups were fed basal diets supplemented with Cu from copper sulphate and copper methionine. Copper concentrations of the experimental diets were 20 and 40 mg Cu/kg dry matter. A metabolism trial of 4 days was conducted during the last week of experimental feeding. Neither Cu source nor Cu level affected average daily gain, average daily feed intake or feed gain ratio (P > 0.05). Broilers fed 40 mg Cu/kg diets had lower plasma cholesterol than those in the control group (P < 0.05). Copper supplementation decreased (P < 0.05) plasma low-density lipoprotein cholesterol but did not alter plasma high-density lipoprotein cholesterol concentrations or plasma triglyceride concentrations. Copper sulphate supplementation increased (P < 0.05) liver Cu but did not alter pectorals Cu, heart Cu, tibia Cu and tibia P. Broilers fed 40 mg/kg Cu from copper sulphate had a lower (P < 0.05) tibia Ca level. The concentration of liver Cu in the broilers fed copper methionine diets was higher (P < 0.05) than that in those fed copper sulphate. Compared with copper sulphate (100%), the relative bioavailability value of copper methionine was 117%. In conclusion, the relative bioavailability of copper methionine obtained in this study was greater than that of copper sulphate. Copper plays an important role in plasma lipids and in the digestion of dietary Ca in broiler chickens.

Anwar M.N., Ravindran V. (2016): Chapter 12 Measurement of calcium digestibility in feed ingredients for poultry methodology and challenges. In: Walk C.L., Kuhn I., Stein H.H., Kidd M.T., Rodehutscord M. (eds): Phytate Destruction – Consequences for Precision Animal Nutrition. Wageningen Academic Publishers, Wageningen, the Netherlands, 191–206.
AOAC (2005): Official Methods of Analysis. 18th Ed. Association of Official Analytical Chemists, Gaithersburg, USA.
Braude R. (1945): Some observations on the need for copper in the diet of fattening pigs. The Journal of Agricultural Science, 35, 163–167.
Bunce G.E. (1993): Hypercholesterolemia of copper deficiency is linked to glutathione metabolism and regulation of hepatic HMG-CoA reductase. Nutrition Reviews, 51, 305–307.
Chowdhury S.D., Paik I.K., Namkung H., Lim H.S. (2004): Responses of broiler chickens to organic copper fed in the form of copper-methionine chelate. Animal Feed Science and Technology, 115, 281–293.
Coble K.F., DeRouchey J.M., Tokach M.D., Dritz S.S., Goodband R.D., Woodworth J.C., Usry J.L. (2017): The effects of copper source and concentration on growth performance, carcass characteristics, and pen cleanliness in finishing pigs. Journal of Animal Science, 95, 4052–4059.
Cresswell G., Nair N., Evans J. (1990): Effect of boron and copper contaminants in poultry manure on the growth of the common mushroom, Agaricus bisporus. Australian Journal of Experimental Agriculture, 30, 707–712.
Cui H., Nie H., Zhang T.T., Wang Z.C., Gao X.H., Yang F.H., Xing X.M., Shi B. (2018): Effects of zinc sources and levels on zinc bioavailability, blood parameters, and nutrient balance of male mink (Neovison vison). Czech Journal of Animal Science, 63, 174–181.
Di Giancamillo A., Rossi R., Martino P.A., Aidos L., Maghin F., Domeneghini C., Corino C. (2018): Copper sulphate forms in piglet diets: Microbiota, intestinal morphology and enteric nervous system glial cells. Animal Science Journal, 89, 616–624.
Echeverry H., Yitbarek A., Munyaka P., Alizadeh M., Cleaver A., Camelo-Jaimes G., Wang P., O.K., Rodriguez-Lecompte J.C. (2016): Organic trace mineral supplementation enhances local and systemic innate immune responses and modulates oxidative stress in broiler chickens. Poultry Science, 95, 518–527.
Flis M., Gugala D., Muszynski S., Dobrowolski P., Kwiecien M., Grela E.R., Tomaszewska E. (2019): The influence of the partial replacing of inorganic salts of calcium, zinc, iron, and copper with amino acid complexes on bone development in male pheasants from aviary breeding. Animals, 9, 237.
Hu Y., Cheng H., Tao S. (2017): Environmental and human health challenges of industrial livestock and poultry farming in China and their mitigation. Environment International, 107, 111–130.
Kim S., Chao P.Y., Allen K.G. (1992): Inhibition of elevated hepatic glutathione abolishes copper deficiency cholesterolemia. The FASEB Journal, 6, 2467–2471.
Konjufca V.H., Pesti G.M., Bakalli R.I. (1997): Modulation of cholesterol levels in broiler meat by dietary garlic and copper. Poultry Science, 76, 1264–1271.
Li C., Guo J., Pan J., Su R., Tang Z., Yang F., Cao H. (2017): Liver mitochondrial dysfunction and electron transport chain defect induced by high dietary copper in broilers. Poultry Science, 96, 3298–3304.
Littell R.C., Henry P.R., Lewis A.J., Ammerman C.B. (1997): Estimation of relative bioavailability of nutrients using SAS procedures. Journal of Animal Science, 75, 2672–2683.
Liu Z., Wu X., Zhang T., Guo J., Gao X., Yang F., Xing X. (2015): Effects of dietary copper and zinc supplementation on growth performance, tissue mineral retention, antioxidant status, and fur quality in growing-furring blue foxes (Alopex lagopus). Biological Trace Element Research, 168, 401–410.
Liu Z., Wu X., Zhang T., Cui H., Guo J., Guo Q., Gao X., Yang F. (2016): Influence of dietary copper concentrations on growth performance, serum lipid profiles, antioxidant defenses, and fur quality in growing-furring male blue foxes (Vulpes lagopus). Journal of Animal Science, 94, 1095–1104.
Lu L., Hao S., Zhang L., Luo X. (2013): Effect of copper source on phytase stability in the premix of weanling piglets. Animal Production Science, 53, 142–145.
Manangi M.K., Vazquez-Anon M., Richards J.D., Carter S., Buresh R.E., Christensen K.D. (2012): Impact of feeding lower levels of chelated trace minerals versus industry levels of inorganic trace minerals on broiler performance, yield, footpad health, and litter mineral concentration. Journal of Applied Poultry Research, 21, 881–890.
Mayer A.N., Berwanger E., Lopes M., Ebbing M.A., Vieira S.L., Angel C.R., Kindlein L. (2018): Copper requirements of broiler breeder hens. Poultry Science, 97, 2785–2797.
Minervino A.H.H., Lopez-Alonso M., Barreto Jr. R.A., Rodrigues F.A.M.L., Araujo C.A.S.C., Sousa R.S., Mori C.S., Miranda M., Oliveira F.L.C., Antonelli A.C., Ortolani E.L. (2018): Dietary zinc supplementation to prevent chronic copper poisoning in sheep. Animals, 8, 227.
Mondal M.K., Das T.K., Biswas P., Samanta C.C., Bairagi B. (2007): Influence of dietary inorganic and organic copper salt and level of soybean oil on plasma lipids, metabolites and mineral balance of broiler chickens. Animal Feed Science and Technology, 139, 212–233.
Nicholson F.A., Chambers B.J., Williams J.R., Unwin R.J. (1999): Heavy metal contents of livestock feeds and animal manures in England and Wales. Bioresource Technology, 70, 23–31.
Ognik K., Sembratowicz I., Cholewinska E., Jankowski J., Kozlowski K., Juskiewicz J., Zdunczyk Z. (2018): The effect of administration of copper nanoparticles to chickens in their drinking water on the immune and antioxidant status of the blood. Animal Science Journal, 89, 579–588.
Olukosi O.A., van Kuijk S., Han Y. (2018): Copper and zinc sources and levels of zinc inclusion influence growth performance, tissue trace mineral content, and carcass yield of broiler chickens. Poultry Science, 97, 3891–3898.
Palenikova I., Hauptmanova K., Pitropovska E., Palenik T., Husakova T., Pechova A., Pavlata L. (2014): Copper metabolism in goat–kid relationship at supplementation of inorganic and organic forms of copper. Czech Journal of Animal Science, 59, 201–207.
Ptak A., Bedford M.R., Swiatkiewicz S., Zyla K., Jozefiak D. (2015): Phytase modulates ileal microbiota and enhances growth performance of the broiler chickens. PLoS One, 10, e0119770.
Singh A.K., Ghosh T.K., Haldar S. (2015): Effects of methionine chelate- or yeast proteinate-based supplement of copper, iron, manganese and zinc on broiler growth performance, their distribution in the tibia and excretion into the environment. Biological Trace Element Research, 164, 253–260.
Star L., van der Klis J.D., Rapp C., Ward T.L. (2012): Bioavailability of organic and inorganic zinc sources in male broilers. Poultry Science, 91, 3115–3120.
Wang Z., Yu H., Wu X., Zhang T., Cui H., Wan C., Gao X. (2016): Effects of dietary zinc pectin oligosaccharides chelate supplementation on growth performance, nutrient digestibility and tissue zinc concentrations of broilers. Biological Trace Element Research, 173, 475–482.
Wu X., Liu Z., Zhang T., Yang Y., Yang F., Gao X. (2014a): Effects of dietary copper on nutrient digestibility, tissular copper deposition and fur quality of growing-furring mink (Mustela vison). Biological Trace Element Research, 158, 166–175.
Wu X.Z., Yang Y., Liu H.T., Yue Z.Y., Gao X.H., Yang F.H., Xing X. (2014b): Effects of dietary copper supplementation on nutrient digestibility, serum biochemical indices, and growth rate of young female mink (Neovison vison). Czech Journal of Animal Science, 59, 529–537.
Wu X.Z., Zhang T.T., Guo J.G., Liu Z., Yang F.H., Gao X.H. (2015): Copper bioavailability, blood parameters, and nutrient balance in mink. Journal of Animal Science, 93, 176–184.
Wu X., Dai S., Hua J., Hu H., Wang S., Wen A. (2018): Influence of dietary copper methionine concentrations on growth performance, digestibility of nutrients, serum lipid profiles, and immune defenses in broilers. Biological Trace Element Research, 191, 199–206.
Zatulovskaia Y.A., Ilyechova E.Y., Puchkova L.V. (2015): The features of copper metabolism in the rat liver during development. PLoS One, 10, e0140797.
Zhang F., Zheng W., Guo R., Yao W. (2017): Effect of dietary copper level on the gut microbiota and its correlation with serum inflammatory cytokines in Sprague-Dawley rats. Journal of Microbiology, Immunology and Infection, 55, 694–702.
Zhao Y., Wang D., Yang S. (2016): Effect of organic and conventional rearing system on the mineral content of pork. Meat Science, 118, 103–107.
download PDF

© 2020 Czech Academy of Agricultural Sciences