Analysis of the relationship between caecal flora difference and production performance of two rabbit species by high-throughput sequencing

https://doi.org/10.17221/225/2020-CJASCitation:

Guo Z.Q., Wang B., Lu J.Z., Li C.Y., Kuang L.D., Tang X.X., Mei X.L., Xie X.H. (2021): Analysis of the relationship between caecal flora difference and production performance of two rabbit species by high-throughput sequencing. Czech J. Anim. Sci.,66:271-280.

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The purpose of this experiment is to study the relationship between the difference in production performance between Sichuan White (SC) rabbits and New Zealand (NZL) rabbits and the diversity of caecal flora. Twelve pregnant SC rabbits and 12 NZL female rabbits were selected for this experiment. After delivery, the young rabbits were divided into two groups according to breeds, each group had 30 replicates, and each replicate had one rabbit. During the experiments, these rabbits were kept in the same room, and the temperature in the room was controlled at 12–25 °C, with a 16-hour light cycle every 24 hours. The nutritional composition of the feed and other environmental conditions were consistent. On the 59th day of the experiment, the caecum contents of the two groups of young rabbits were collected. The results showed that the survival rate of the SC rabbit group was higher than that of the NZL rabbit group, and the diarrhoea rate and average daily gain were lower than those of the NZL rabbit group (P < 0.05). The results of high-throughput sequencing of the 16S gene showed that compared with the NZL rabbit group, the relative abundance of Bacteroides increased, and the abundance of harmful flora Verrucomicrobia and Proteobacteria decreased (P < 0.05). Functional analysis of the microflora showed that the relative abundance of carbohydrate metabolism genes in the SC rabbit group was higher than in the NZL rabbit group. In conclusion, compared with the NZL rabbits, the SC rabbits have a more optimized intestinal flora structure and lower abundance of harmful bacteria. Moreover, the intestinal health level of SC rabbits is improved, and the tolerance to roughage of SC rabbits is increased.

References:
Chen Y, Zhao B, Wu Y, Hu S, Mu L, Zhu C, Pan Y, Wu X. Impacts of diarrhea on the immune system, intestinal environment, and expression of PGRPs in New Zealand rabbits. PeerJ. 2017 Nov 27;5: 16 p. https://doi.org/10.7717/peerj.4100
 
Eshar D, Weese JS. Molecular analysis of the microbiota in hard feces from healthy rabbits (Oryctolagus cuniculus) medicated with long term oral meloxicam. BMC Vet Res. 2014 Dec;10(1): 9 p. https://doi.org/10.1186/1746-6148-10-62
 
Faith JJ, Guruge JL, Charbonneau M, Subramanian S, Seedorf H, Goodman AL, Clemente JC, Knight R, Heath AC, Leibel RL, Rosenbaum M, Gordon JI. The long-term stability of the human gut microbiota. Science. 2013 Jul 5;341(6141): 19 p. https://doi.org/10.1126/science.1237439
 
Fan P, Bian B, Teng L, Nelson CD, Driver J, Elzo MA, Jeong KC. Host genetic effects upon the early gut microbiota in a bovine model with graduated spectrum of genetic variation. ISME J. 2020 Jan;14(1):302-17. https://doi.org/10.1038/s41396-019-0529-2
 
Frese SA, Parker K, Calvert CC, Mills DA. Diet shapes the gut microbiome of pigs during nursing and weaning. Microbiome. 2015 Dec;3(1): 10 p. https://doi.org/10.1186/s40168-015-0091-8
 
Fu L, Jiang B, Liu J, Zhao X, Liu Q, Hu X. Genome sequence analysis of a flocculant-producing bacterium, Paenibacillus shenyangensis. Biotechnol Lett. 2016 Mar 1;38(3):447-53.  https://doi.org/10.1007/s10529-015-1990-2
 
Guarner F, Malagelada JR. Gut flora in health and disease. Lancet. 2003 Feb 8;361(9356):512-9. https://doi.org/10.1016/S0140-6736(03)12489-0
 
Koropatkin NM, Cameron EA, Martens EC. How glycan metabolism shapes the human gut microbiota. Nat Rev Microbiol. 2012 May;10(5):323-35.  https://doi.org/10.1038/nrmicro2746
 
Langille MG, Zaneveld J, Caporaso JG, McDonald D, Knights D, Reyes JA, Clemente JC, Burkepile DE, Thurber RL, Knight R, Beiko RG, Huttenhower C. Predictive functional profiling of microbial communities using 16S rRNA marker gene sequences. Nat Biotechnol. 2013 Sep; https://doi.org/10.1038/nbt.2676
 
31(9):814-21.
 
Musso G, Gambino R, Cassader M. Obesity, diabetes, and gut microbiota: The hygiene hypothesis expanded. Diabetes Care. 2010 Oct 1;33(10):2277-84.  https://doi.org/10.2337/dc10-0556
 
Quast C, Pruesse E, Yilmaz P, Gerken J, Schweer T, Yarza P, Peplies J, Glockner FO. The SILVA ribosomal RNA gene database project: Improved data processing and web-based tools. Nucleic Acids Res. 2013 Jan 1;41(D1):D590-6.  https://doi.org/10.1093/nar/gks1219
 
Rhee KJ, Sethupathi P, Driks A, Lanning DK, Knight KL. Role of commensal bacteria in development of gut-associated lymphoid tissues and preimmune antibody repertoire. J Immunol. 2004 Jan 15;172(2):1118-24. https://doi.org/10.4049/jimmunol.172.2.1118
 
Rogowski A, Briggs JA, Mortimer JC, Tryfona T, Terrapon N, Lowe EC, Basle A, Morland C, Day AM, Zheng H, Rogers TE, Thompson P, Hawkins AR, Yadav MP, Henrissat B, Martens EC, Dupree P, Gilbert HJ, Bolam DN. Glycan complexity dictates microbial resource allocation in the large intestine. Nat Commun. 2015 Jun 26;6(1): 16 p.  https://doi.org/10.1038/ncomms8481
 
Shin NR, Whon TW, Bae JW. Proteobacteria: microbial signature of dysbiosis in gut microbiota. Trends Biotechnol. 2015 Sep 1;33(9):496-503.  https://doi.org/10.1016/j.tibtech.2015.06.011
 
Skrivanova E, Molatova Z, Skrivanova V, Marounek M. Inhibitory activity of rabbit milkand medium-chain fatty acids against enteropathogenic Escherichia coli O128. Vet Microbiol. 2009 Mar 30;135(3-4):358-62.  https://doi.org/10.1016/j.vetmic.2008.09.083
 
Spor A, Koren O, Ley R. Unravelling the effects of the environment and host genotype on the gut microbiome. Nat Rev Microbiol. 2011 Apr;9(4):279-90.  https://doi.org/10.1038/nrmicro2540
 
Turturice BA, Gold DR, Litonjua AA, Oken E, Rifas-Shiman S, Perkins DL, Finn PW. Lower perinatal exposure to Proteobacteria is an independent predictor of early childhood wheezing. J Allergy Clin Immunol. 2018 Jan 14;143(1):419-21. https://doi.org/10.1016/j.jaci.2018.06.051
 
Wang C, Zhu Y, Li F, Huang L. The effect of Lactobacillus isolates on growth performance, immune response, intestinal bacterial community composition of growing Rex Rabbits. J Anim Physiol Anim Nutr (Berl). 2017 Oct 8;101(5):e1-13. https://doi.org/10.1111/jpn.12629
 
Yang R, Ye G, Yang XY, Dong Q, Li GL, Li SC, Zhang M, Wang WY. [Effects of different concentrations of Chinese herbal compound on the growth of Sichuan White rabbits]. Genom Appl Biol. 2019, Nov 16;(01):93-9. Chinese
 
Yu T. [Study on the microflora of intestinal lumen of piglets of different breeds]. Chin J Vet Sci. 2016 Nov 21;271. Chinese.
 
Zhao L. The gut microbiota and obesity: from correlation to causality. Nat Rev Microbiol. 2013 Sep 29;11(9):639-47.  https://doi.org/10.1038/nrmicro3089
 
Zhao X, Xian Y, Li C, Wang C, Yu D, Zhu W, Hang S. [Feeding Lactobacillus plantarum and Lactobacillus casei increased microbial diversity and short chain fatty acids production in the gut-intestinal tract of weaning piglets]. Wei Sheng Wu Xue Bao. 2016 Aug 4;56(8):1291-300. Chinese with English abstract.
 
Zitomersky NL, Atkinson BJ, Franklin SW, Mitchell PD, Snapper SB, Comstock LE, Bousvaros A. Characterization of adherent bacteroidales from intestinal biopsies of children and young adults with inflammatory bowel disease. PLOS One. 2013 Jun 11;8(6): 11 p. https://doi.org/10.1371/journal.pone.0063686
 
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