The aim of the present research was to determine the changes in macromineral and trace element concentrations in ewes’ blood during the lactation period. Thirty-six Travnik pramenka ewes at the average age of 3–5 years were included in research and they were evaluated at three stages of lactation: early stage (40 ± 5 days of lactation), medium stage (80 ± 5 days of lactation) and late stage (120 ± 5 days of lactation). In ewes’ blood concentrations of macroelements (Ca, P, K, Na, Mg) and trace elements (Fe, Cu, Zn, Mo, Co, Se, Cd, As) were determined. The average concentrations of macroelements and trace elements in the blood of ewes during lactation were mostly within the reference values. Se concentrations in blood were close to the deficient ones, while concentrations of Cd and As were negligible. Concentration of Ca in blood was the lowest in the early lactation stage, higher in the late stage, and the highest in the medium stage (88.25 mg/l, 96.43 mg/l and 103.43 mg/l, respectively). A significant increase in the concentrations of Mg, Co and Cd and a significant decrease in the concentrations of Na, Fe, Cu, Zn, Mo and Se were found in blood with lactation progress. Significant correlations between the studied parameters were found, especially between macroelements (Ca : K, P : Na, K : Na, K : Mg). Positive correlations were found between some macroelements and trace elements (Ca : Co, Ca : Cd, K : Co, Na : Zn, Na : Se) and between trace elements (Fe : Cu, Cu : Zn, Cu : Mo, Cu : Se, Zn : Se, Mo : Se and Co : As). Negative correlations were found between Ca and Cu, Ca and Mo, P and Cd, K and Cu. Obtained results may help in monitoring the nutritional status and health of ewes during the lactation period, providing a clearer idea related to the supply of macrominerals and trace elements to ewes. Besides, results indicated that the environment of the farming area was not polluted with toxic elements.
Abd-El Naser EM, Mohamed GAE, Elsayed HK. Effect of lactation stages on some blood serum biochemical parameters and milk composition in dairy cows. Assiut Vet Med J. 2014 Aug;60(142):83-8.
Abdelrahman MM, Aljumaah RS. Metabolic blood profiles and milk compositions of peri-parturient and early lactation periods in sheep. Asian J Anim Vet Adv. 2012 Nov;7(11):1130-8. https://doi.org/10.3923/ajava.2012.1130.1138
Ademi A, Bernhoft A, Govasmark E, Bytyqi H, Sivertsen T, Singh BR. Selenium and other mineral concentrations in feed and sheep’s blood in Kosovo. Transl Anim Sci. 2017 Feb;1(1):97-107. https://doi.org/10.2527/tas2016.0010
Antunovic Z, Novoselec J, Klir Z. Hematological parameters in ewes during lactation in organic farming. Poljoprivreda. 2017a Nov;23(2):46-52. https://doi.org/10.18047/poljo.23.2.7
Antunovic Z, Novoselec J, Speranda M, Steiner Z, Cavar S, Pavlovic N, Valek Lendic K, Mioc B, Pacinovski N, Klir Z. Monitoring of blood metabolic profile and milk quality of ewes during lactation in organic farming. Mljekarstvo. 2017b Sep;67(4):243-52. https://doi.org/10.15567/mljekarstvo.2017.0401
Broucek J, Soch M, Srejberova P. Effect of different environmental factors on selected blood minerals in sheep. Slovak J Anim Sci. 2009 Sep 30;42(2):145-50.
EU 2010. Directive 2010/63/EU of the European Parliament and of the Council of 22 September 2010 on the protection of animals used for scientific purposes. OJEU. 2010 Oct 20;L276: 47 p.
El Zubeir EMI, Elowni OAO, Mohamed GE. Correlation of minerals and enzymes in blood serum and milk of healthy and mastitic cows. Res J Agric Biol Sci. 2005 Jan;1(1):45-9.
Haenlein GFW, Anke M. Mineral and trace element research in goats. A review. Small Rumin Res. 2011 Jan 1;95(1):2-19. https://doi.org/10.1016/j.smallrumres.2010.11.007
Herdt TH, Hoff B. The use of blood analysis to evaluate trace mineral status in ruminant livestock. Vet Clin North Am Food Anim Pract. 2011 Jul 1;27(2):255-83. https://doi.org/10.1016/j.cvfa.2011.02.004
Hu W, Murphy MR. Dietary cation-anion difference effects on performance and acid-base status of lactating dairy cows: A meta-analysis. J Dairy Sci. 2004 Jul 1;87(7):2222-9. https://doi.org/10.3168/jds.S0022-0302(04)70042-9
Humann-Ziehank E, Tegtmeyer PC, Seelig B, Roehrig P, Ganter M. Variation of serum selenium concentrations in German sheep flocks and implications for herd health management consultancy. Acta Vet Scand. 2013 Nov 19;55(1): [8 p.]. https://doi.org/10.1186/1751-0147-55-82
Jukna C, Jukna V, Suigzdaite J. Determination of heavy metals in viscera and muscles of cattle. Bulg J Vet Med. 2006 Jan;9(1):35-41.
Kaneko JJ, Harvey JW, Bruss ML. Clinical biochemistry of domestic animals. 6th ed. London: Elsevier/Academic Press; 2008. 931 p.
Kovacik A, Arvay J, Tusimova E, Harangozo L, Tvrda E, Zbynovska K, Cupka P, Andrascikova S, Tomas J, Massanyi P. Seasonal variations in the blood concentration of selected heavy metals in sheep and their effects on the biochemical and hematological parameters. Chemosphere. 2017 Feb 1;168:365-71. https://doi.org/10.1016/j.chemosphere.2016.10.090
Kulcu R, Yur F. A study of some serum mineral levels before and during pregnancy and during lactation period of sheep and cattle. Biol Trace Elem Res. 2003 Jun;92(3):275-80. https://doi.org/10.1385/BTER:92:3:275
Lopez-Alonso M. Trace minerals and livestock: Not too much not too little. ISRN Vet Sci. 2012 Dec;2012: [18 p.]. https://doi.org/10.5402/2012/704825
Novoselec J, Gregurincic I, Klir Z, Mioc B, Siric I, Drzaic V, Antunovic Z. The estimation of body weight from body measurements of Travnik Pramenka sheep in the area of Bilogora, Croatia. J Cent Eur Agr. 2020 Jun;21(2):207-14. https://doi.org/10.5513/JCEA01/21.2.2667
NRC – National Research Council. Nutrient requirements of small ruminants. Washington, D.C.: The National Academy Press; 2007. 347 p.
Page CM, Murphy TW, Van Emon ML, Bowman JGP, Wyffels SA, Stewart WC. Blood serum mineral element concentrations of weaned Montana ram lambs and their relationship with water quality characteristics. Prof Anim Sci. 2018 Oct 1;34(5):410-20. https://doi.org/10.15232/pas.2018-01747
Popenga RH, Ramsey J, Gonzales BJ, Johnson CK. Reference intervals for mineral concentrations in whole blood and serum of bighorn sheep (Ovis canadensis) in California. J Vet Diagn Invest. 2012 May;24(3):531-8. https://doi.org/10.1177/1040638712441936
Puls R. Mineral levels in animal health: Diagnostic data. 2nd ed. Clearbrook (BC): Sherpa International; 1994. p. 302-53.
Ramirez-Perez AH, Buntix SE, Rosiles R. Effect of breed and age on the voluntary intake and the micromineral status of non-pregnant sheep. II. Micromineral status. Small Rumin Res. 2000 Aug 1;37(3):231-42. https://doi.org/10.1016/S0921-4488(00)00121-8
Schweinzer V, Iwersen M, Drillich M, Wittek M, Tichy A, Mueller A, Krametter-Froetscher R. Macromineral and trace elements supply in sheep and goats in Austria. Vet Med-Czech. 2017 Feb 13;62(2):62-73. https://doi.org/10.17221/243/2015-VETMED
Sobiech P, Milewski S, Zdunczyk S. Yield and composition of milk and blood biochemical components of ewes nursing a single lamb or twins. Bull Vet Inst Pulawy. 2008 Aug;52(4):591-6.
Suttle NF. Mineral nutrition of livestock. 4th ed. Wallingford: CABI Publishing; 2010. 587 p.
Underwood EJ, Suttle NF. The mineral nutrition of livestock. 3rd ed. Wallingford: CABI Publishing; 1999. 614 p.