The effect of milking frequency on milk yield and milk composition in ewesčuhová L., Tančin V., Mačuhová J. (2020): The effect of milking frequency on milk yield and milk composition in ewes. Czech J. Anim. Sci., 65: 41-50.
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Milking frequency is the main factor regulating milk yield and milk quality if feeding, welfare, health, and environmental conditions are adequate. Milk yield and composition are substantially influenced by breed, and consequently the breed has an impact on both factors at a different frequency of milking. Further, the size of cisternal compartment plays an important role in accommodating secreted milk between milkings. Thus, ewes with large cisterns (i.e. large cisternal area, high cisternal milk percentage, and delayed tight junction opening during milk stasis) adapt themselves better to longer milking intervals than ewes with a small cistern. The increase of milking frequency from two to three times a day can lead to an increase of milk yield in the range of 3 to 36%. On the other hand, the reduction of milking frequency from twice to once a day can decrease milk yield from 9 to 67%. Two milkings per week could be omitted with no negative effects on milk yield, milk composition, and somatic cell count in ewes with large cisterns. However, besides the breed and cistern storage capacity, the results in literature show that the effects of milking frequency on milk yield and milk composition can vary according to the stage of lactation, individual animal, production level, practice of stripping, and duration of changed frequency. The right use of different milking frequency strategies can result in increased milk yield or in significant savings in labour and time spent in the milking parlour with negligible or no negative effects on milk yield and composition.

Albenzio M, Santillo A, Caroprese M, Ruggieri D, Ciliberti M, Sevi A. Immune competence of the mammary gland as affected by somatic cell and pathogenic bacteria in ewes with subclinical mastitis. J. Dairy Sci. 2012;95:3877-3887.
Antonic J, Tancin V, Uhrincat M, Macuhova L, Macuhova J, Jackuliakova L. The effect of exogenous oxytocin on milkability and milk composition in ewes differed in milk flow pattern. Small Rumin. Res. 2013a;113:254-257.
Antonic J, Macuhova L, Uhrincat M, Tancin V. The effect of milk ejection occurrence before or during machine milking on milkability and milk composition of ewes. Vet. Zootec. 2013b;61:3-7.
Antonic J, Jackuliakova L, Uhrincat M, Macuhova L, Oravcova M, Tancin V. Changes in milk yield and composition after lamb weaning and start of machine milking in dairy ewes. Slovak J. Anim. Sci. 2013c;46:93-99.
Ayadi M, Matar AM, Aljumaah RS, Alshaikh MA, Abouheif MA. Evolution of udder morphology, alveolar and cisternal milk compartment during lactation and their relationship with milk yield in Najdi sheep. Span. J. Agric. Res. 2014;12:1061-1070.
Baranovic S, Tancin V, Tvarozkova K, Uhrincat M, Macuhova L, Palkovic J. Impact of somatic cell count and lameness on production and composition of ewe’s milk. Potravinarstvo Slovak J. Food Sci. 2018;12:116-122.
Bencini R, Pulina G. The quality of sheep milk: A review. Aust. J. Exp. Agric. 1997;37:485-504.
Bortacki P, Kujawiak R, Czerniawska-Piatkowska E, Kirdar SS, Wojcik J, Grzesiak W. Impact of milking frequency on yield, chemical composition and quality of milk in high producing dairy herd. Mljekarstvo. 2017;67:226-230.
Bruckmaier RM, Paul G, Mayer H, Schams D. Machine milking of Ostfriesian and Lacaune dairy sheep: udder anatomy, milk ejection, and milking characteristics. J. Dairy Res. 1997;64:163-172.
Caja G, Ayadi M, Knight CH. Changes in cisternal compartment based on stage of lactation and time since milk ejection in the udder of dairy cows. J. Dairy Sci. 2004;87:2409-2415.
Castillo V, Such X, Caja G, Salama AAK, Albanell E, Casals R. Mid-term lactational effects of once- versus twice-daily milking in Manchega and Lacaune dairy ewes. J. Dairy Sci. 2005;88:286-287.
Castillo V, Such X, Caja G, Salama AAK, Albanell E, Casals R. Changes in alveolar and cisternal compartments induced by milking interval in the udder of dairy ewes. J. Dairy Sci. 2008;91:3403-3411.
Castillo V, Such X, Caja G, Casals R, Salama AAK, Albanell E. Long- and short-term effects of omitting two weekend milkings on the lactational performance and mammary tight junction permeability of dairy ewes. J. Dairy Sci. 2009;92:3684-3695.
Davis SR, Farr VC, Copeman PJA, Carruthers VR, Knight CH, Stewagen K. Partitioning of milk accumulation between cisternal and alveolar compartments of the bovine udder: Relationship to production loss during once daily milking. J. Dairy Sci. 1998;65:1-8.
De Bie L, Berger YM, Thomas DL. The effect of three times a day milking at the beginning of lactation on the milk production of East Friesian crossbred ewes. Proc. 6th Great Lakes, Dairy Sheep Symposium, Guelph, Canada; 2000. pp. 1-9.
Gelasakis AI, Arsenos G, Valergakis GE, Oikonomou G, Kiossis E, Fthenakis GC. Study of factors affecting udder traits and assessment of the interrelationships with milking efficiency in Chios breed ewes. Small Rumin. Res. 2012;103:232-239.
Giadinis ND, Arsenos G, Tsakos P, Psychas V, Dovas CI, Papadopoulos E, Karatzias H, Fthenakis GC. “Milk-drop syndrome of ewes”: Investigation of the causes in dairy sheep in Greece. Small Rumin. Res. 2012;106:33-35.
Gonzalez-Garcia E, Tesniere A, Camous S, Bocquier F, Barillet F, Hassoun P. The effects of parity, litter size, physiological state, and milking frequency on the metabolic profile of Lacaune dairy ewes. Domest. Anim. Endocrin. 2015;50:32-44.
Hervas G, Ramella JL, Lopez S, Gonzalez JS, Mantecon A. Effect of omitting one or two milkings weekly on lactational performance in dairy ewes. J. Dairy Res. 2006;73:207-215.
Knight TW, Gosling LS. Effect of milking frequency and machine-stripping on the yield and composition of milk from Poll Dorset ewes. New Zeal. J. Agr. Res. 1995;38:123-130.
Koutsouli P, Smitzis P, Theodorou G, Massouras T, Bizelis I, Politis I. The effect of milking frequency reduction from twice to once daily on mammary physiology and animal welfare of two dairy Greek sheep breeds. Small Rumin. Res. 2017;147:18-24.
Krpalkova L, Cabrera VE, Zavadilova L, Stipkova M. The importance of hoof health in dairy production. Czech J. Anim. Sci. 2019;64:107-117.
Labussiere J. Review of physiological and anatomical factors influencing the milking ability of ewes and the organization of milking. Livest. Prod. Sci. 1988;18:253-274.
Macuhova L, Uhrincat M, Macuhova J, Margetin M, Tancin V. The first observation of milkability of the sheep breeds Tsigai, Improved Valachian and their crosses with Lacaune. Czech J. Anim. Sci. 2008a;53:528-536.
Macuhova L, Uhrincat M, Broucek J, Tancin V. Reaction of primiparous dairy cows reared in early postnatal period in different systems on milking conditions. Slovak J. Anim. Sci. 2008b;41:98-104.
Macuhova L, Tancin V, Uhrincat M, Macuhova J. The level of udder emptying and milk flow stability in Tsigai, Improved Valachian, and Lacaune ewes during machine milking. Czech J. Anim. Sci. 2012;57:240-247.
Margetin M, Spanik J, Milerski M, Capistrak A, Apolen D. Relationship between morphological and functional parameters of udder and somatic cell counts in milk of ewes. In: Tancin V, Mihina S, Uhrincat M, editors. Physiological and Technical Aspects of Machine Milking. Proc. International Conference, Nitra, Slovak Republic; 2005. pp. 255-258.
Marnet PG, McKusick BC. Regulation of milk ejection and milkability in small ruminants. Livest. Prod. Sci. 2001;70:125-133.
Marnet PG, Komara M. Management systems with extended milking intervals in ruminants: Regulation of production and quality of milk. J. Anim. Sci. 2008;86:47-56.
Masar M, Mikus M. [The effect of simplified milking of Tsigai on milk production and milk composition]. Vedecké práce Výskumneho ústavu ovčiarskeho v Trenčíně. 1985;12:99-107. Slovak.
McKusick BC, Thomas DL, Berger YM. Effect of weaning system on commercial milk production and lamb growth of East Friesian dairy sheep. J. Dairy Sci. 2001;84:1660-1668.
McKusick BC, Thomas DL, Berger YM, Marnet PG. Effect of milking interval on alveolar versus cisternal milk accumulation and milk production and composition in dairy ewes. J. Dairy Sci. 2002a;85:2197-2206.
McKusick BC, Thomas DL, Romero JE, Marnet PG. Effect of weaning system on milk composition and distribution of milk fat within the udder of East Friesian dairy ewes. J. Dairy Sci. 2002b;85:2521-2528.
Mikus M, Masar M. Milk production and labour productivity in sheep milking twice and thrice daily and without stripping. In: Proc. 2nd International Symposium on Machine Milking of Small Ruminants, Alghero, Italy; 1978. pp. 263-276.
Milerski M, Margetin M, Capistrak A, Apolen D, Spanik J, Oravcova M. Relationships between external and internal udder measurements and the linear scores for udder morphology traits in dairy sheep. Czech J. Anim. Sci. 2006;51:383-390.
Negrao JA, Marnet PG, Labussiere J. Effect of milking frequency release and milk production in dairy ewes. Small Rumin. Res. 2001;39:181-187.
Nudda A, Bencini R, Mijatovic S, Pulina G. The yield and composition of milk in Sarda, Awassi, and Merino sheep milked unilaterally at different frequencies. J. Dairy Sci. 2002;85:2879-2884.
O’Brien BC, Ryan WJ, Meaney D, McDonagh D, Kelly A. Effect of frequency of milking on yield, composition and processing quality of milk. J. Dairy Res. 2002;69:367-374.
Oravcova M, Margetin M, Tancin V. The effect of stage of lactation on daily milk yield, and milk fat and protein content in Tsigai and Improved Valachian ewes. Mljekarstvo. 2015;65:48-56.
Papachristoforou C, Roushias A, Mavrogenis AP. The effect of milking frequency on the milk production of Chios ewes and Damascus goats. Ann. Zootech. 1982;31:37-46.
Peris C, Diaz JD, Balasch S, Beltran MC, Molina MP, Fernandez N. Influence of vacuum level and overmilking on udder health and teat thickness changes in dairy ewes. J. Dairy Sci. 2003a;86:3891-3898.
Peris C, Diaz JR, Segura C, Marti A, Fernandez N. Influence of pulsation rate on udder health and teat thickness changes in dairy ewes. J. Dairy Sci. 2003b;86:530-537.
Politis I, Block E, Turner JD. Effect of somatotropin on the plasminogen and plasmin system in the mammary gland: Proposed mechanism of action for somatotropin on the mammary gland. J. Dairy Sci. 1990;73:1494-1499.
Prieto N, Bodas R, Lopez-Campos O, Andres S, Giraldez FJ. Effect of sunflower oil supplementation and milking frequency reduction on sheep milk production and composition. J. Anim. Sci. 2013;91:446-454.
Pulina G, Nudda A, Macciotta NPP, Battacone G, Rassu SPG, Cannas A. Non-nutritional factors affecting persistency in dairy ewes. It. J. Anim. Sci. 2007;6:115-141.
Raynal-Ljutovac K, Pirisi A, de Cremoux R, Gonzalo C. Somatic cells of goat and sheep milk: Analytical, sanitary, productive and technological aspects. Small Rumin. Res. 2007;68:126-144.
Roca A, Romero G, Alejandro M, Muelas R, Diaz JR. Milk electrical conductivity in Manchega ewes: Variation throughout milking and relation with mammary gland health status. Czech J. Anim. Sci. 2019;64:300-308.
Rovai M, Such X, Piedrafita J, Caja G, Pujol MR. Evolution of mammary morphology traits during lactation and its relationship with milk yield of Manchega and Lacaune dairy sheep. Proc. 6th International Symposium on the Milking of Small Ruminants, Athens, Greece; 1999. pp. 107-109.
Rovai M, Caja G, Such X. Evaluation of udder cisterns and effects on milk yield of dairy ewes. J. Dairy Sci. 2008;91:4622-4629.
Rovai M, Rusek N, Caja G, Saldo J, Leitner G. Effect of subclinical intramammary infection on milk quality in dairy sheep: I. Fresh-soft cheese produced from milk of uninfected and infected glands and from their blends. Small Rumin. Res. 2015;125:127-136.
Salama AAK, Caja G, Such X, Peris S, Sorensen A, Knight CH. Changes in cisternal udder compartment induced by milking interval in dairy goats milked once or twice daily. J. Dairy Sci. 2004;87:1181-1187.
Santibanez A, Such X, Caja G, Castillo V, Albanell E. Lactational effects of once- versus twice-daily milkings throughout lactation in dairy ewes. Book of Abstracts of the 60th Annual Meeting of the EAAP, Barcelona, Spain; 2009. p. 470.
Shamay A, Shapiro R, Mabjeesh SJ, Silanikove N. Casein-derived phosphopeptides disrupt tight junction integrity, and precipitously dry up milk secretion in goats. Life Sci. 2002;70:2707-2719.
Shamay A, Shapiro F, Leitner G, Silanikove N. Infusions of casein hydrolyzates into the mammary gland disrupt tight junction integrity and induce involution in cows. J. Dairy Sci. 2003;86:1250-1258.
Silanikove N, Shamay A, Shinder D, Moran A. Stress down regulates milk yield in cows by plasmin Induced β-casein product that blocks K+ channels on the apical membranes. Life Sci. 2000;67:2201-2212.
Silanikove N, Leitner G, Merin U, Prosser CG. Recent advances in exploiting goat’s milk: Quality, safety and production aspects. Small Rumin. Res. 2010;89:111-124.
Sinapis E, Marnet PG, Skapetas B, Hatziminaoglou I. Vacuum level for opening the teat sphincter and the change of the teat end wall thickness during the machine milking of mountainous Greek breed (Boutsiko) ewes. Small Rumin. Res. 2007;69:242-246.
Stelwagen K, Farr VC, Davis SR, Prosser CG. EGTA-induced disruption of epithelial cell tight junctions in the lactating caprine mammary gland. Am. J. Physiol. 1995;269:848-855.
Tancin V, Uhrincat M, Macuhova L, Bruckmaier RM. Effect of pre-stimulation on milk flow pattern and distribution of milk constituents at a quarter level. Czech J. Anim. Sci. 2007;52:117-121.
Thomas DL, Berger YM, McKusick BC, Mikolayunas CM. Dairy sheep production research at the University of Wisconsin-Madison, USA – a review. J. Anim. Sci. Biotechnol. 2014;5:22.
Torres A, Castro N, Arguello A, Capote J. Comparison between two milk distribution structures in dairy goats milked at different milking frequencies. Small Rumin. Res. 2013;114:161-166.
Tvarozkova K, Tancin V, Holko I, Uhrincat M, Macuhova L. Mastitis in ewes: Somatic cell counts, pathogens and antibiotic resistance. JMBFS. 2019;9:661-670.
Wall EH, McFadden TB. Triennial lactation symposium: A local affair: How the mammary gland adapts to changes in milking frequency. J. Anim. Sci. 2012;90:1695-1707.
Wilde CJ, Henderson AJ, Knight CH, Blatchford DR, Faulkner A, Vernon RG. Effects of long-term thrice-daily milking on mammary enzyme activity, cell population and milk yield in the goat. J. Anim. Sci. 1987;64:533-539.
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