Effect of low-density lipoprotein addition to soybean lecithin-based extenders on bull spermatozoa following freezing-thawing – preliminary results

https://doi.org/10.17221/27/2016-CJASCitation:Šimoník O., Rajmon R., Stádník L., Šichtař J., Beran J., Ducháček J., Hodek P., Trefil P. (2016): Effect of low-density lipoprotein addition to soybean lecithin-based extenders on bull spermatozoa following freezing-thawing – preliminary results. Czech J. Anim. Sci., 61: 560-567.
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Soybean lecithin-based extenders represent an alternative to extenders containing egg yolk, but there has been discussion about their cryoprotective efficacy. Low-density lipoprotein (LDL) was proved as a cryoprotective agent, which can replace egg yolk in extenders. The aim of this study was to investigate the effects of LDL addition to the soybean extenders on their cryoprotective properties. The effect of the LDL of our production was verified using commercial egg yolk extender BULLXcell®, 6%, 8%, and 10% LDL (v/v) as an egg yolk replacement. The effects of LDL addition to the soybean lecithin-based extenders in concentrations of 4%, 6%, and 8% (v/v) were tested using extenders AndroMed® and Bioxcell®. In total, 64 samples from eight bulls were evaluated. Kinematic parameters of spermatozoa, resulting from Computer Assisted Sperm Analysis, and their viability, evaluated by fluorescent technique, were assessed immediately after thawing and after 2 hours. The quality of LDL compared to other studies was confirmed, and the beneficial effects of egg yolk replacement by LDL were proved in extender BULLXcell®. 8% LDL provided the best values for the majority of kinematic parameters (P < 0.05), without effect on total motility (P > 0.05). Furthermore, addition of 4%, 6%, and 8% LDL to the soybean lecithin-based extender Bioxcell® showed a positive effect on the majority of kinematic parameters of spermatozoa (P < 0.05) at both times of incubation. However there was no significant influence on total motility (P > 0.05). Viability was higher after thawing in the case of 8% LDL (P < 0.05). However, there was no consistent effect of LDL addition to the AndroMed® extender. In conclusion, cryoprotective properties of the semen extenders based on a soybean lecithin can be improved by the addition of LDL.
Aires Viviana A., Hinsch Klaus-Dieter, Mueller-Schloesser Frank, Bogner Katja, Mueller-Schloesser Susanne, Hinsch Elvira (2003): In vitro and in vivo comparison of egg yolk-based and soybean lecithin-based extenders for cryopreservation of bovine semen. Theriogenology, 60, 269-279  https://doi.org/10.1016/S0093-691X(02)01369-9
Alomar M., Alzoabi M., Zarkawi M. (): Kinetics of hydrogen peroxide generated from live and dead ram spermatozoa and the effects of catalase and oxidase substrates addition. Czech Journal of Animal Science, 61, 1-7  https://doi.org/10.17221/8662-CJAS
Amirat L., Anton M., Tainturier D., Chatagnon G., Battut I., Courtens J. L. (): Modifications of bull spermatozoa induced by three extenders: Biociphos, low density lipoprotein and Triladyl, before, during and after freezing and thawing. Reproduction, 129, 535-543  https://doi.org/10.1530/rep.1.00011
Amirat-Briand L., Bencharif D., Vera-Munoz O., Bel Hadj Ali H., Destrumelle S., Desherces S., Schmidt E., Anton M., Tainturier D. (2009): Effect of glutamine on post-thaw motility of bull spermatozoa after association with LDL (low density lipoproteins) extender: Preliminary results. Theriogenology, 71, 1209-1214  https://doi.org/10.1016/j.theriogenology.2008.10.002
Amirat-Briand L., Bencharif D., Vera-Munoz O., Pineau S., Thorin C., Destrumelle S., Desherces S., Anton M., Jouan M., Shmitt E., Tainturier D. (2010): In vivo fertility of bull semen following cryopreservation with an LDL (low density lipoprotein) extender: Preliminary results of artificial inseminations. Animal Reproduction Science, 122, 282-287  https://doi.org/10.1016/j.anireprosci.2010.09.006
Beran J., Stadnik L., Bezdicek J., Louda F., Citek J., Duchacek J. (2012): Effect of sire and extender on sperm motility and share of live or dead sperm in bull’s fresh ejaculate and in AI doses after thawing. Archiv Tierzücht, 3, 207–218.
Beran J., Stádník L., Ducháček J., Okrouhlá M., Doležalová M., Kadlecová V., Ptáček M. (2013): Relationships among the cervical mucus urea and acetone, accuracy of insemination timing, and sperm survival in Holstein cows. Animal Reproduction Science, 142, 28-34  https://doi.org/10.1016/j.anireprosci.2013.09.005
Bergeron A. (2003): Low-Density Lipoprotein Fraction from Hen's Egg Yolk Decreases the Binding of the Major Proteins of Bovine Seminal Plasma to Sperm and Prevents Lipid Efflux from the Sperm Membrane. Biology of Reproduction, 70, 708-717  https://doi.org/10.1095/biolreprod.103.022996
Crespilho A.M., Sá Filho M.F., Dell'Aqua J.A., Nichi M., Monteiro G.A., Avanzi B.R., Martins A., Papa F.O. (2012): Comparison of in vitro and in vivo fertilizing potential of bovine semen frozen in egg yolk or new lecithin based extenders. Livestock Science, 149, 1-6  https://doi.org/10.1016/j.livsci.2012.05.011
Drobnis Erma Z., Crowe Lois M., Berger Trish, Anchordoguy Thomas J., Overstreet James W., Crowe John H. (1993): Cold shock damage is due to lipid phase transitions in cell membranes: A demonstration using sperm as a model. Journal of Experimental Zoology, 265, 432-437  https://doi.org/10.1002/jez.1402650413
Dzyuba V., Cosson J., Dzyuba B., Rodina M. (): Oxidative stress and motility in tench Tinca tinca spermatozoa. Czech Journal of Animal Science, 60, 250-262  https://doi.org/10.17221/8238-CJAS
Gil J, Januskauskas A, Haard MCh, Haard Mgm, Johanisson A, Soderquist L, Rodriguez-Martinez H (2000): Functional Sperm Parameters and Fertility of Bull Semen Extended in Biociphos-PlusR and TriladylR. Reproduction in Domestic Animals, 35, 69-77  https://doi.org/10.1046/j.1439-0531.2000.00197.x
Harrison R. A. P., Vickers S. E. (1990): Use of fluorescent probes to assess membrane integrity in mammalian spermatozoa. Reproduction, 88, 343-352  https://doi.org/10.1530/jrf.0.0880343
Hu Jian-Hong, Li Qing-Wang, Zan Lin-Sen, Jiang Zhong-Liang, An Jun-Hui, Wang Li-Qiang, Jia Yong-Hong (2010): The cryoprotective effect of low-density lipoproteins in extenders on bull spermatozoa following freezing–thawing. Animal Reproduction Science, 117, 11-17  https://doi.org/10.1016/j.anireprosci.2009.04.001
Hu Jian-Hong, Jiang Zhong-Liang, Lv Rui-Kai, Li Qing-Wang, Zhang Shu-Shan, Zan Lin-Sen, Li Yao-Kun, Li Xin (2011): The advantages of low-density lipoproteins in the cryopreservation of bull semen. Cryobiology, 62, 83-87  https://doi.org/10.1016/j.cryobiol.2010.12.007
Hyldgaard M., Mygind T., Vad B. S., Stenvang M., Otzen D. E., Meyer R. L. (): The Antimicrobial Mechanism of Action of Epsilon-Poly-L-Lysine. Applied and Environmental Microbiology, 80, 7758-7770  https://doi.org/10.1128/AEM.02204-14
Koshimoto Chihiro, Gamliel Edna, Mazur Peter (2000): Effect of Osmolality and Oxygen Tension on the Survival of Mouse Sperm Frozen to Various Temperatures in Various Concentrations of Glycerol and Raffinose. Cryobiology, 41, 204-231  https://doi.org/10.1006/cryo.2000.2281
Meamar M., Shahneh A.Z., Zamiri M.J., Zeinoaldini S., Kohram H., Hashemi M.R., Asghari S. (): Preservation effects of melatonin on the quality and fertility of native Fars rooster semen during liquid storage. Czech Journal of Animal Science, 61, 42-48  https://doi.org/10.17221/8667-CJAS
Moussa M, Martinet V, Trimeche A, Tainturier D, Anton M (2002): Low density lipoproteins extracted from hen egg yolk by an easy method: cryoprotective effect on frozen–thawed bull semen. Theriogenology, 57, 1695-1706  https://doi.org/10.1016/S0093-691X(02)00682-9
Muchlisin Z.A., Nadiah W.N., Nadiya N., Fadli N., Hendri A., Khalil M., Siti-Azizah M.N. (): Exploration of natural cryoprotectants for cryopreservation of African catfish, Clarias gariepinus, Burchell 1822 (Pisces: Clariidae) spermatozoa. Czech Journal of Animal Science, 60, 10-15  https://doi.org/10.17221/7906-CJAS
Pace M. M., Graham E. F. (1974): Components in Egg Yolk which Protect Bovine Spermatozoa during Freezing. Journal of Animal Science, 39, 1144-  https://doi.org/10.2527/jas1974.3961144x
Sieme H, Oldenhof H, Wolkers WF (2015): Sperm Membrane Behaviour during Cooling and Cryopreservation. Reproduction in Domestic Animals, 50, 20-26  https://doi.org/10.1111/rda.12594
Špaleková Eliška, Makarevich Alexander V., Kubovičová Elena, Ostró Alexander, Chrenek Peter (2014): Effect of caffeine on functions of cooling-stored ram sperm in vitro. Acta Veterinaria Brno, 83, 19-25  https://doi.org/10.2754/avb201483010019
Stádník Luděk, Rajmon Radko, Beran Jan, Šimoník Ondřej, Doležalová Martina, Šichtař Jiří, Stupka Roman, Folková Petra (2015): Influence of selected factors on bovine spermatozoa cold shock resistance. Acta Veterinaria Brno, 84, 125-131  https://doi.org/10.2754/avb201584020125
Thun Rico, Hurtado Maria, Janett F (2002): Comparison of Biociphos-Plus® and TRIS-egg yolk extender for cryopreservation of bull semen. Theriogenology, 57, 1087-1094  https://doi.org/10.1016/S0093-691X(01)00704-X
van Wagtendonk-de Leeuw A.M., Haring R.M., Kaal-Lansbergen L.M.T.E., den Daas J.H.G. (2000): Fertility results using bovine semen cryopreserved with extenders based on egg yolk and soy bean extract. Theriogenology, 54, 57-67  https://doi.org/10.1016/S0093-691X(00)00324-1
Vera-Munoz O., Amirat-Briand L., Diaz T., Vásquez L., Schmidt E., Desherces S., Anton M., Bencharif D., Tainturier D. (2009): Effect of semen dilution to low-sperm number per dose on motility and functionality of cryopreserved bovine spermatozoa using low-density lipoproteins (LDL) extender: Comparison to Triladyl® and Bioxcell®. Theriogenology, 71, 895-900  https://doi.org/10.1016/j.theriogenology.2008.10.010
Verstegen J., Iguer-Ouada M., Onclin K. (2002): Computer assisted semen analyzers in andrology research and veterinary practice. Theriogenology, 57, 149-179  https://doi.org/10.1016/S0093-691X(01)00664-1
Vishwanath R., Shannon P. (2000): Storage of bovine semen in liquid and frozen state. Animal Reproduction Science, 62, 23-53  https://doi.org/10.1016/S0378-4320(00)00153-6
Wall R.J., Foote R.H. (1999): Fertility of Bull Sperm Frozen and Stored in Clarified Egg Yolk-Tris-Glycerol Extender. Journal of Dairy Science, 82, 817-821  https://doi.org/10.3168/jds.S0022-0302(99)75301-4
Zhang X.-G., Hong J.-Y., Yan G.-J., Wang Y.-F., Li Q.-W., Hu J.-H. (): Association of heat shock protein 70 with motility of&nbsp;frozen-thawed sperm in bulls. Czech Journal of Animal Science, 60, 256-262  https://doi.org/10.17221/8239-CJAS
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