Two mitochondrial genes are associated with performance traits in farmed raccoon dogs (Nyctereutes procyonoides) S., Slaska B., Zieba G., Rozempolska-Rucinska I. (2018): Two mitochondrial genes are associated with performance traits in farmed raccoon dogs (Nyctereutes procyonoides)  . Czech J. Anim. Sci., 63: 110-118.
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The relationships between chosen mitochondrial genes polymorphisms and performance traits in raccoon dogs were determined. The study involved 354 farmed raccoon dogs. Blood collected from the animals was the analysed biological material. Mitochondrial DNA genes, i.e. MT-CO1 (mitochondrially encoded cytochrome c oxidase I), MT-CO2 (mitochondrially encoded cytochrome c oxidase II), and MT-CYB (mitochondrially encoded cytochrome b) were amplified using the polymerase chain reaction method. The amplicons obtained were sequenced and subjected to bioinformatics analysis. Based on the nucleotide sequences, three haplotypes for the MT-CO1 gene fragment and two haplotypes for the MT-CO2 gene fragment were identified. The sequence of the MT-CYB gene was monomorphic. Based on the haplotypes, five previously undescribed mitochondrial haplogroups were determined. Statistical analysis revealed significant differences between the values of three of the five investigated performance traits of raccoon dogs and the identified haplotypes and mitochondrial haplogroups, taking into account predictors of direct additive effects, additive maternal effects, and fixed specific maternal environmental effects. The new mitochondrial haplogroups identified in the farmed raccoon dog population may imply constant emergence of adaptive mutations that are conserved in subsequent generations. The results of the association study indicate a statistically significant association between haplotypes and mitochondrial haplogroups of farmed raccoon dogs and their body weight, body size, and colour type, which allows considering MT-CO1 and MT-CO2 genes as candidate genes encoding these traits in raccoon dogs. The results of the molecular analyses can be applied to improve the performance traits in farmed raccoon dogs.

Chen Jianxing, Song Zhenhua, Rong Meijie, Min Lingjiang, Sun Yujiang (2009): The association analysis between Cytb polymorphism and growth traits in three Chinese donkey breeds. Livestock Science, 126, 306-309
CSHZ (1997): Standard for Raccoon Dog Performance Evaluation. Central Animal Breeding Office, Warszawa, Poland. (in Polish)
Fernández A. I., Alves E., Fernández A., de Pedro E., López-García M. A., Ovilo C., Rodríguez M. C., Silió L. (2008): Mitochondrial genome polymorphisms associated with longissimus muscle composition in Iberian pigs1. Journal of Animal Science, 86, 1283-1290
Gugolek A., Lorek M.O., Zablocka D. (2002): Studies on the relationship between body weight, trunk length and pelt size in arctic fox. Czech Journal of Animal Science, 47, 328–332.
Jeon G. J., Chung H. Y., Choi J. G., Lee M. S., Lee C. W., Park J. J., Ha J. M., Lee H. K., Sung H. H. (2005): Relationship between Genetic Variants of Mitochondrial DNA and Growth Traits in Hanwoo Cattle. Asian-Australasian Journal of Animal Sciences, 18, 301-307
Mannen H., Morimoto M., Oyama K., Mukai F., Tsuji S. (2003): Identification of mitochondrial DNA substitutions related to meat quality in Japanese Black cattle1,2. Journal of Animal Science, 81, 68-73
Misztal I., Tsuruta S., Strabel T., Auvray B., Druet T., Lee D.H. (2002): BLUPF90 and related programs (BGF90). In: Proc. 7th World Congress on Genetics Applied to Livestock Production, Montpellier, France, Comm. No. 28–07.
Nisztuk-Pacek Sylwia (2016): Genetic variability of farmed fur animals of the Canidae family assessed by nuclear and mitochondrial DNA analysis. Medycyna Weterynaryjna, 72, 505-510
Nørgaard Louise Solveig, Mikkelsen Dorthe Marlene Götz, Elmeros Morten, Chriél Mariann, Madsen Aksel Bo, Nielsen Jeppe Lund, Pertoldi Cino, Randi Ettore, Fickel Joerns, Brygida Slaska, Ruiz-González Aritz (2017): Population genomics of the raccoon dog (Nyctereutes procyonoides) in Denmark: insights into invasion history and population development. Biological Invasions, 19, 1637-1652
Reicher S., Seroussi E., Weller J. I., Rosov A., Gootwine E. (2012): Ovine mitochondrial DNA sequence variation and its association with production and reproduction traits within an Afec-Assaf flock1. Journal of Animal Science, 90, 2084-2091
Slaska Brygida, Grzybowska-Szatkowska Ludmiła (2011): Analysis of the mitochondrial haplogroups of farm and wild-living raccoon dogs in Poland. Mitochondrial DNA, 22, 105-110
Slaska B., Grzybowska-Szatkowska L., Bugno-Poniewierska M., Surdyka M., Smiech A. (2013): Nuclear and mitochondrial DNA mutation in human and canine tumors. Medycyna Weterynaryjna, 69, 195–202.
Slaska B., Zieba G., Rozempolska-Rucinska I., Jezewska-Witkowska G., Nisztuk S., Horecka B., Zon A. (2016): Mitochondrial DNA haplotypes are associated with utility traits in raccoon dogs. Animal Science Papers and Reports, 34, 293–302.
Surdyka M., Slaska B. (2016a): Defect of the mitochondrial DNA hypervariable region as a risk factor for canine mammary tumour. Veterinary and Comparative Oncology, 15, 820–828.
Surdyka M., Slaska B. (2016b): Defect in ND2, COX2, ATP6, and COX3 mitochondrial genes as a risk factor for canine mammary tumour. Veterinary and Comparative Oncology, 15, 1062–1072.
Sutarno , Cummins J.M., Greeff J., Lymbery A.J. (2002): Mitochondrial DNA polymorphisms and fertility in beef cattle. Theriogenology, 57, 1603-1610
Wierzbicki H., Filistowicz A. (2001): Grading versus animal model evaluation in arctic fox (Alopex lagopus). Czech Journal of Animal Science, 46, 252–259.
Yen N-T, Lin C-S, Ju C-C, Wang S-C, Huang M-C (2007): Mitochondrial DNA Polymorphism and Determination of Effects on Reproductive Trait in Pigs. Reproduction in Domestic Animals, 42, 387-392
Zhang Bao, Chen Hong, Hua Liushuai, Zhang Chunlei, Kang Xiangtao, Wang Xinzhuang, Pan Chuanying, Lan Xianyong, Lei Chuzhao (2008): Novel SNPs of the mtDNA ND5 Gene and Their Associations with Several Growth Traits in the Nanyang Cattle Breed. Biochemical Genetics, 46, 362-368
Zhuo Y., Zhou L.N., Teng L.S., Wang J.F., Yuan M.P., Tang X.W., Zheng B.J., Xu L. (2016): Mitochondrial haplotypes and the human mitochondrial diseases. Progress in Biochemistry and Biophysics, 43, 1070–1075.
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