Estimation of genetic parameters of fatty acids composition in flesh of market size common carp (Cyprinus carpio L.) and their relation to performance traits revealed that selective breeding can indirectly affect flesh quality M., Vandeputte M., Gela D., Doležal M., Buchtová H., Rodina M., Flajšhans M., Kocour M. (2018): Estimation of genetic parameters of fatty acids composition in flesh of market size common carp (Cyprinus carpio L.) and their relation to performance traits revealed that selective breeding can indirectly affect flesh quality  . Czech J. Anim. Sci., 63: 280-291.
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Fish are a rich source of omega-3 polyunsaturated fatty acids (n-3 PUFAs) and thus, they should be an integral part of human diet at least twice a week. As a result, high attention has been devoted to the improvement of fatty acids (FA) content in the flesh of farmed fish through nutrition. Conversely, there are very few data on the potential of selective breeding to improve FA composition in fish. We estimated genetic parameters of fillet fatty acid content and performance traits in market size common carp cultured under semi-intensive pond conditions. The experimental stock arose through factorial mating of 7 dams and 36 sires. All families were reared communally. Pedigree was reconstructed with microsatellite markers, and 158 individuals were dressed out and selected for flesh FA composition analysis. Heritability estimates of total muscle fat, FA composition in total fat (TF) (n-3 PUFA-TF, PUFA-TF, EPA-TF – eicosapentaenoic acid, n-6/n-3 – omega6/omega3 PUFA ratio), and most performance traits were moderately heritable (h² = 0.23–0.41), and body weight was highly heritable (h2 = 0.62 ± 0.20). Genetic correlations show that selection for faster growth would indirectly lead to fillet yield improvement (rg = 0.50–0.62) while having little impact on muscle fat (rg = 0.21). However, lipid quality in flesh would be affected: n-3 PUFA-TF would decrease and the n-6/n-3 PUFA ratio would increase. A likely interpretation is that faster growing genotypes consume more supplemental feed, which was poor in the beneficial FAs. For sustainable selective breeding, supplemental feed composition should be modified, so that faster growing carps would maintain an appropriate flesh quality.

Anton-Pardo M., Adámek Z. (2015): The role of zooplankton as food in carp pond farming: a review. Journal of Applied Ichthyology, 31, 7-14
Chavanne Hervé, Janssen Kasper, Hofherr Johann, Contini Franca, Haffray Pierrick, Komen Hans, Nielsen Einar Eg, Bargelloni Luca (2016): A comprehensive survey on selective breeding programs and seed market in the European aquaculture fish industry. Aquaculture International, 24, 1287-1307
Dupont-Nivet Mathilde, Vandeputte Marc, Chevassus Bernard (2002): Optimization of factorial mating designs for inference on heritability in fish species. Aquaculture, 204, 361-370
Folch J., Lees M., Stanley G.H.S. (1957): A simple method for the isolation and purification of total lipids from animal tissues. Journal of Biological Chemistry, 226, 497–509.
Fontagne-Dicharry S., Medale F. (2010): Lipids of aquaculture fish species and their variation factors. OCL – Oilseeds and fats, Crops and Lipids, 17, 209–213. (in French)
FOSS Analytical AB (2003): Application note 390/revision 2.8/2007: Total Fat Determination Using SoxCap™ 2047 in Combination with Soxtec Extraction Systems. FOSS Analytical AB, Höganäs, Sweden.
García-Celdrán M., Ramis G., Manchado M., Estévez A., Afonso J.M., Armero E. (2015): Estimates of heritabilities and genetic correlations of carcass quality traits in a reared gilthead sea bream (Sparus aurata L.) population sourced from three broodstocks along the Spanish coasts. Aquaculture, 446, 175-180
Groeneveld E., Kovac M., Mielenz N. (2008): VCE – User’s Guide and Reference Manual – Version 6.0. Institute of Farm Animal Genetics, Neustadt, Germany. Available at (accessed Aug 1, 2010).
Hlaváč David, Adámek Zdeněk, Hartman Pavel, Másílko Jan (2014): Effects of supplementary feeding in carp ponds on discharge water quality: a review. Aquaculture International, 22, 299-320
Horvath L., Tamas G., Seagrave C. (1992): Carp and Pond Fish Culture including Chinese Herbivorous Species, Pike, Tench, Zander, Wels Catfish and Goldfish. Fishing News Books Ltd., Oxford, UK.
Janssen K., Chavanne H., Berentsen P., Komen H. (2017): Impact of selective breeding on European aquaculture. Aquaculture, 472, 8-16
Kause Antti, Kiessling Anders, Martin Samuel A. M., Houlihan Dominic, Ruohonen Kari (2016): Genetic improvement of feed conversion ratio via indirect selection against lipid deposition in farmed rainbow trout (Oncorhynchus mykiss Walbaum). British Journal of Nutrition, 116, 1656-1665
Kocour Martin, Mauger Stéphane, Rodina Marek, Gela David, Linhart Otomar, Vandeputte Marc (2007): Heritability estimates for processing and quality traits in common carp (Cyprinus carpio L.) using a molecular pedigree. Aquaculture, 270, 43-50
Leaver Michael J., Taggart John B., Villeneuve Laure, Bron James E., Guy Derrick R., Bishop Stephen C., Houston Ross D., Matika Oswald, Tocher Douglas R. (2011): Heritability and mechanisms of n−3 long chain polyunsaturated fatty acid deposition in the flesh of Atlantic salmon. Comparative Biochemistry and Physiology Part D: Genomics and Proteomics, 6, 62-69
Madsen P., Jensen J. (2013): DMU Version 6. Available at (accessed Dec 1, 2013).
Marković Zoran, Stanković Marko, Rašković Božidar, Dulić Zorka, Živić Ivana, Poleksić Vesna (2016): Comparative analysis of using cereal grains and compound feed in semi-intensive common carp pond production. Aquaculture International, 24, 1699-1723
Mráz J., Pickova J. (2009): Differences between lipid content and composition of different parts of fillets from crossbred farmed carp (Cyprinus carpio). Fish Physiology and Biochemistry, 35, 615-623
Mraz J., Pickova J. (2011): Factors influencing fatty acid composition of common carp (Cyprinus carpio) muscle. Neuroendocrinology Letters, 32, 3–8.
Mraz J., Zajic T., Pickova J. (2012a): Culture of common carp (Cyprinus carpio) with defined flesh quality for prevention of cardiovascular diseases using finishing feeding strategy. Neuroendocrinology Letters, 33, 60–67.
Mraz J., Machova J., Kozak P., Pickova J. (2012b): Lipid content and composition in common carp – optimization of n-3 fatty acids in different pond production systems. Journal of Applied Ichthyology, 28, 238–244.
Nguyen Nguyen Hong, Ponzoni Raul W., Yee Hoong Yip, Abu-Bakar Khairul R., Hamzah Azhar, Khaw Hooi Ling (2010): Quantitative genetic basis of fatty acid composition in the GIFT strain of Nile tilapia (Oreochromis niloticus) selected for high growth. Aquaculture, 309, 66-74
Overturf Ken, Welker Tom, Barrows Frederick, Towner Richard, Schneider Rich, LaPatra Scott (2013): Variation in Rainbow Trout, Oncorhynchus mykiss, to Biosynthesize Eicosapentaenoic Acid and Docosahexaenoic Acid When Reared on Plant Oil Replacement Feeds. Journal of the World Aquaculture Society, 44, 326-337
Pinheiro J.C., Bates D.M. (2000): Mixed-Effects Models in S and S-PLUS. Springer-Verlag, New York, USA.
Prchal Martin, Kause Antti, Vandeputte Marc, Gela David, Allamellou Jean-Michel, Kumar Girish, Bestin Anastasia, Bugeon Jérôme, Zhao Jinfeng, Kocour Martin, Xu Peng (2018): The genetics of overwintering performance in two-year old common carp and its relation to performance until market size. PLOS ONE, 13, e0191624-
Rodrigues Bruna Leal, Canto Anna Carolina Vilhena da Cruz Silva, Costa Marion Pereira da, Silva Flávio Alves da, Mársico Eliane Teixeira, Conte-Junior Carlos Adam, Loor Juan J. (2017): Fatty acid profiles of five farmed Brazilian freshwater fish species from different families. PLOS ONE, 12, e0178898-
Saillant Eric, Dupont-Nivet Mathilde, Sabourault Marie, Haffray Pierrick, Laureau Stanislas, Vidal Marie-Odile, Chatain Béatrice (2009): Genetic variation for carcass quality traits in cultured sea bass ( Dicentrarchus labrax ). Aquatic Living Resources, 22, 105-112
Steffens Werner (2016): Aquaculture produces wholesome food: cultured fish as a valuable source of n-3 fatty acids. Aquaculture International, 24, 787-802
Tocher Douglas R. (2010): Fatty acid requirements in ontogeny of marine and freshwater fish. Aquaculture Research, 41, 717-732
Trbovic D., Zivic I., Stankovic M., Zivic M., Dulic Z., Petronijevic R., Markovic Z. (2017): Dependence of the common carp (Cyprinus carpio L.) fatty acid profile on diet composition in a semi-intensive farming system: tissue and time variability. Aquaculture Research, 48, 3121–3133.
Vandeputte Marc, Kocour Martin, Mauger Stéphane, Dupont-Nivet Mathilde, De Guerry Daphné, Rodina Marek, Gela David, Vallod Dominique, Chevassus Bernard, Linhart Otomar (2004): Heritability estimates for growth-related traits using microsatellite parentage assignment in juvenile common carp (Cyprinus carpio L.). Aquaculture, 235, 223-236
VANDEPUTTE M., MAUGER S., DUPONT-NIVET M. (2006): An evaluation of allowing for mismatches as a way to manage genotyping errors in parentage assignment by exclusion. Molecular Ecology Notes, 6, 265-267
Vandeputte Marc, Kocour Martin, Mauger Stéphane, Rodina Marek, Launay Amandine, Gela David, Dupont-Nivet Mathilde, Hulak Martin, Linhart Otomar (2008): Genetic variation for growth at one and two summers of age in the common carp (Cyprinus carpio L.): Heritability estimates and response to selection. Aquaculture, 277, 7-13
Vandeputte Marc, Garouste Romain, Dupont-Nivet Mathilde, Haffray Pierrick, Vergnet Alain, Chavanne Hervé, Laureau Stanislas, Ron Tetsuzan Benny, Pagelson Glen, Mazorra Carlos, Ricoux Rémi, Marques Pedro, Gameiro Marta, Chatain Béatrice (2014): Multi-site evaluation of the rearing performances of 5 wild populations of European sea bass (Dicentrarchus labrax). Aquaculture, 424-425, 239-248
Zajic T., Mraz J., Sampels S., Pickova J. (2013): Fillet quality changes as a result of purging of common carp (Cyprinus carpio L.) with special regard to weight loss and lipid profile. Aquaculture, 400–401, 111–119.
Zheng X., Seiliez I., Hastings N., Tocher D.R., Panserat S., Dickson C.A., Bergot P., Teale A.J. (2004): Characterization and comparison of fatty acyl Δ6 desaturase cDNAs from freshwater and marine teleost fish species. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 139, 269-279
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