Association of miR-208b polymorphism with meat quality traits and texture parameters in pigs

https://doi.org/10.17221/31/2018-CJASCitation:Polasik D., Tyra M., Szyndler-Nędza M., Żak G., Lambert B., Terman A. (2018): Association of miR-208b polymorphism with meat quality traits and texture parameters in pigs. Czech J. Anim. Sci., 63: 435-442.
download PDF

Previous studies have shown that single nucleotide polymorphism (SNP) (rs328743478) located downstream of pre-miR-208b in MYH7 gene is correlated with fibre number composition and drip loss in pigs. Because fibre characteristics are linked to meat quality, the aim of this study was to analyse the relationships between rs328743478 polymorphism and meat quality and texture parameters. The study utilised 578 pigs belonging to Polish Landrace, Polish Large White, Puławska, Pietrain, Duroc, and Hampshire breeds. Association study was performed for the first three breeds separately and for all six breeds joined together. Obtained results showed a significant influence (P ≤ 0.05) of miR-208b genotypes on water holding capacity (WHC) in the whole population and individual breeds and on intramuscular fat content (IMF) and luminosity in the whole population as well as in Landrace and Puławska breeds, respectively. By analysing texture parameters, we found association (P ≤ 0.05 or P ≤ 0.01) between rs328743478 polymorphism and toughness, firmness, and chewiness measured in m. semimembranosus and m. longissimus lumborum. The highest values of these traits in m. semimembranosus were noticed for AA genotype, however in m. longissimus lumborum for GG in the whole population and some individual breeds. It was found that miR-208b genotypes were also associated with cohesiveness (P ≤ 0.01), springiness, and hardness (P ≤ 0.05) but obtained results were not consistent among breeds and the whole population. The obtained results confirm that miR-208b polymorphism is associated with some economically important traits in pigs.

References:
Aken B.L., Ayling S., Barrell D., Clarke L., Curwen V., Fairley S., Fernandez Banet J., Billis K., Garcia Giron C., Hourlier T., Howe K., Kahari A., Kokocinski F., Martin F.J., Murphy D.N., Nag R., Ruffier M., Schuster M., Tang Y.A., Vogel J.H., White S., Zadissa A., Flicek P., Searle S.M.J. (2016): The Ensembl gene annotation system. Database: The Journal of Biological Databases and Curation, 2016: baw093.
 
Bartel David P (2004): MicroRNAs. Cell, 116, 281-297  https://doi.org/10.1016/S0092-8674(04)00045-5
 
Fu Y., Li L., Ren S. (2013): Effect of FTO expression and polymorphism on fat deposition in Suzhong pigs. Asian-Australasian Journal of Animal Sciences, 26, 1365–1373.
 
Gentry J. G., McGlone J. J., Miller M. F., Blanton J. R. (2004): Environmental effects on pig performance, meat quality, and muscle characteristics1. Journal of Animal Science, 82, 209-217  https://doi.org/10.2527/2004.821209x
 
Hamm R. (1986): Functional properties of the myofibrilar system and their measurement. In: Bechtel P.J. (ed.): Muscle as Food. Academic Press Inc., London, UK, 135–199.
 
Hong Jae-Sang, Noh Su-Hyun, Lee Jun-Seong, Kim Jun-Mo, Hong Ki-Chang, Lee Young Sik (2012): Effects of polymorphisms in the porcine microRNA miR-1 locus on muscle fiber type composition and miR-1 expression. Gene, 506, 211-216  https://doi.org/10.1016/j.gene.2012.06.050
 
Horak Martin, Novak Jan, Bienertova-Vasku Julie (2016): Muscle-specific microRNAs in skeletal muscle development. Developmental Biology, 410, 1-13  https://doi.org/10.1016/j.ydbio.2015.12.013
 
Hou Xinhua, Tang Zhonglin, Liu Honglin, Wang Ning, Ju Huiming, Li Kui, Van Wijnen Andre (2012): Discovery of MicroRNAs Associated with Myogenesis by Deep Sequencing of Serial Developmental Skeletal Muscles in Pigs. PLoS ONE, 7, e52123-  https://doi.org/10.1371/journal.pone.0052123
 
Hwang Young-Hwa, Kim Gap-Don, Jeong Jin-Yeon, Hur Sun-Jin, Joo Seon-Tea (2010): The relationship between muscle fiber characteristics and meat quality traits of highly marbled Hanwoo (Korean native cattle) steers. Meat Science, 86, 456-461  https://doi.org/10.1016/j.meatsci.2010.05.034
 
Jennen D. G. J., Brings A. D., Liu G., Jüngst H., Tholen E., Jonas E., Tesfaye D., Schellander K., Phatsara C. (2007): Genetic aspects concerning drip loss and water-holding capacity of porcine meat. Journal of Animal Breeding and Genetics, 124, 2-11  https://doi.org/10.1111/j.1439-0388.2007.00681.x
 
Joo S.T., Kim G.D., Hwang Y.H., Ryu Y.C. (2013): Control of fresh meat quality through manipulation of muscle fiber characteristics. Meat Science, 95, 828-836  https://doi.org/10.1016/j.meatsci.2013.04.044
 
Kim J. M., Lim K. S., Hong J. S., Kang J. H., Lee Y. S., Hong K. C. (2015): A polymorphism in the porcine miR-208b is associated with microRNA biogenesis and expressions of SOX-6 and MYH7 with effects on muscle fibre characteristics and meat quality. Animal Genetics, 46, 73-77  https://doi.org/10.1111/age.12255
 
Kozomara Ana, Griffiths-Jones Sam (2013): miRBase: annotating high confidence microRNAs using deep sequencing data. Nucleic Acids Research, 42, D68-D73  https://doi.org/10.1093/nar/gkt1181
 
Lee Jun-Seong, Kim Jun-Mo, Lim Kyu-Sang, Hong Jae-Sang, Hong Ki-Chang, Lee Young Sik (2013): Effects of polymorphisms in the porcine microRNA MIR206 / MIR133B cluster on muscle fiber and meat quality traits. Animal Genetics, 44, 101-106  https://doi.org/10.1111/j.1365-2052.2012.02362.x
 
Liu K., Muse S. V. (2005): PowerMarker: an integrated analysis environment for genetic marker analysis. Bioinformatics, 21, 2128-2129  https://doi.org/10.1093/bioinformatics/bti282
 
Ma Jideng, Wang Hongmei, Liu Rui, Jin Long, Tang Qianzi, Wang Xun, Jiang Anan, Hu Yaodong, Li Zongwen, Zhu Li, Li Ruiqiang, Li Mingzhou, Li Xuewei (2015): The miRNA Transcriptome Directly Reflects the Physiological and Biochemical Differences between Red, White, and Intermediate Muscle Fiber Types. International Journal of Molecular Sciences, 16, 9635-9653  https://doi.org/10.3390/ijms16059635
 
MCCARTHY J (2008): MicroRNA-206: The skeletal muscle-specific myomiR. Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms, 1779, 682-691  https://doi.org/10.1016/j.bbagrm.2008.03.001
 
Murgiano Leonardo, Tammen Imke, Harlizius Barbara, Drögemüller Cord (2012): A de novo germline mutation in MYH7 causes a progressive dominant myopathy in pigs. BMC Genetics, 13, 99-  https://doi.org/10.1186/1471-2156-13-99
 
Piórkowska K., Żukowski K., Ropka-Molik K., Tyra M. (2018): Detection of genetic variants between different Polish Landrace and Puławska pigs by means of RNA-seq analysis. Animal Genetics, 49, 215-225  https://doi.org/10.1111/age.12654
 
Quiat D., Voelker K. A., Pei J., Grishin N. V., Grange R. W., Bassel-Duby R., Olson E. N. (2011): Concerted regulation of myofiber-specific gene expression and muscle performance by the transcriptional repressor Sox6. Proceedings of the National Academy of Sciences, 108, 10196-10201  https://doi.org/10.1073/pnas.1107413108
 
Ropka-Molik K., Bereta A., Tyra M., Różycki M., Piórkowska K., Szyndler-Nędza M., Szmatoła T. (2014): Association of calpastatin gene polymorphisms and meat quality traits in pig. Meat Science, 97, 143-150  https://doi.org/10.1016/j.meatsci.2014.01.021
 
Ropka-Molik Katarzyna, Podstawski Przemysław, Piórkowska Katarzyna, Tyra Mirosław (2016): Association of Gene Coding for Microsomal Triglyceride Transfer Protein (MTP) and Meat Texture Characteristic in Pig. Annals of Animal Science, 16, 721-729  https://doi.org/10.1515/aoas-2015-0091
 
Ryu Y.C., Kim B.C. (2005): The relationship between muscle fiber characteristics, postmortem metabolic rate, and meat quality of pig longissimus dorsi muscle. Meat Science, 71, 351-357  https://doi.org/10.1016/j.meatsci.2005.04.015
 
Untergasser Andreas, Cutcutache Ioana, Koressaar Triinu, Ye Jian, Faircloth Brant C., Remm Maido, Rozen Steven G. (2012): Primer3—new capabilities and interfaces. Nucleic Acids Research, 40, e115-e115  https://doi.org/10.1093/nar/gks596
 
van Rooij Eva, Quiat Daniel, Johnson Brett A., Sutherland Lillian B., Qi Xiaoxia, Richardson James A., Kelm Robert J., Olson Eric N. (2009): A Family of microRNAs Encoded by Myosin Genes Governs Myosin Expression and Muscle Performance. Developmental Cell, 17, 662-673  https://doi.org/10.1016/j.devcel.2009.10.013
 
Zak G., Pieszka M. (2009): Improving pork quality through genetics and nutrition. Annals of Animal Science, 9, 327–338.
 
download PDF

© 2019 Czech Academy of Agricultural Sciences