Effects of γ-aminobutyric acid on aggressive behaviour, jejunum villus morphology, serum biochemical indicators and hippocampal neuropeptide mRNA levels in piglets at weaning with mixing

 

https://doi.org/10.17221/33/2018-CJASCitation:Mei H., Yang C., Xie Q., Yang Y., Luo X., Jiao H., Gan L. (2019): Effects of γ-aminobutyric acid on aggressive behaviour, jejunum villus morphology, serum biochemical indicators and hippocampal neuropeptide mRNA levels in piglets at weaning with mixing  . Czech J. Anim. Sci., 64: 151-159.
supplementary materialdownload PDF

The effects of γ-aminobutyric acid (GABA) on weanling piglets after mixing stress were investigated and the underlying molecular mechanism was analyzed. Sixty weaning piglets were randomly assigned to either the control group (weaning and mixing with a 3 : 3 sex ratio) or the GABA supplement group (30 mg GABA/kg body weight/day + weaning and mixing with a 3 : 3 sex ratio). Aggressive behaviours have been recorded for 2 days and the number of lesions for 3 days. The diarrhea rate on day 6 post-weaning and mixing was analyzed. Serum biochemical indicators, antioxidant variables, jejunum villus morphology and mRNA levels of stress-related neuropeptide genes of the hippocampus were investigated. The GABA addition decreased serum adrenocorticotropic hormone concentrations (P < 0.05), aggressive behaviours of weaned piglets 5 h after mixing (P < 0.05), lesion scores over the entire 3-day period (P < 0.01) and diarrhea rate (P < 0.01) and improved jejunum villus integrity. Serum neuropeptide Y (NPY) concentration (P < 0.05) and total superoxide dismutase activity (P < 0.01) were increased in the GABA supplement group, whereas serum malondialdehyde concentration had a decreasing tendency (0.05 < P < 0.1), and glutathione peroxidase activity had an increasing tendency (0.05 < P < 0.1). The GABA treatment group had increased mRNA levels of NPY (P < 0.05) and peptide YY (PYY) (P < 0.05) in the hippocampus, which may contribute to insights into the regulatory mechanism of GABA in weaning and mixing stress. The addition of GABA is beneficial to reduce weaning and mixing stress in piglets, and NPY and PYY may mediate the process.

 

References:
Armstrong D., Browne R. (1994): The analysis of free radicals, lipid peroxides, antioxidant enzymes and compounds related to oxidative stress as applied to the clinical chemistry laboratory. Advances in Experimental Medicine and Biology, 366, 43–58.
 
BAYDAS Giyasettin, SONKAYA Ertugrul, TUZCU Mehmet, YASAR Abdullah, DONDER Emir (2005): Novel role for gabapentin in neuroprotection of central nervous system in streptozotocine-induced diabetic rats1. Acta Pharmacologica Sinica, 26, 417-422  https://doi.org/10.1111/j.1745-7254.2005.00072.x
 
Boudry Gaëlle, Péron Vincent, Le Huërou-Luron Isabelle, Lallès Jean Paul, Sève Bernard (2004): Weaning Induces Both Transient and Long-Lasting Modifications of Absorptive, Secretory, and Barrier Properties of Piglet Intestine. The Journal of Nutrition, 134, 2256-2262  https://doi.org/10.1093/jn/134.9.2256
 
Cai Xuan, Zhu Lihui, Chen Xiaolian, Sheng Yongshuai, Guo Qi, Bao Jian, Xu Jianxiong (2016): X/XO or H2O2 induced IPEC-J2 cell as a new in vitro model for studying apoptosis in post-weaning piglets. Cytotechnology, 68, 713-724  https://doi.org/10.1007/s10616-014-9823-z
 
Clement Juan, Simler Simone, Ciesielski Lucien, Mandel Paul, Cabib Simona, Puglisi-Allegra Stefano (1987): Age-dependent changes of brain GABA levels, turnover rates and shock-induced aggressive behavior in inbred strains of mice. Pharmacology Biochemistry and Behavior, 26, 83-88  https://doi.org/10.1016/0091-3057(87)90538-7
 
D’Eath Richard B (2002): Individual aggressiveness measured in a resident-intruder test predicts the persistence of aggressive behaviour and weight gain of young pigs after mixing. Applied Animal Behaviour Science, 77, 267-283  https://doi.org/10.1016/S0168-1591(02)00077-1
 
Gan Ling, Yang Binyu, Mei Hongyuan (2017): The effect of iron dextran on the transcriptome of pig hippocampus. Genes & Genomics, 39, 1-14  https://doi.org/10.1007/s13258-016-0469-4
 
Grippo Angela J., Lamb Damon G., Carter C. Sue, Porges Stephen W. (2007): Social Isolation Disrupts Autonomic Regulation of the Heart and Influences Negative Affective Behaviors. Biological Psychiatry, 62, 1162-1170  https://doi.org/10.1016/j.biopsych.2007.04.011
 
DEGROOT J, RUIS M, SCHOLTEN J, KOOLHAAS J, BOERSMA W (2001): Long-term effects of social stress on antiviral immunity in pigs. Physiology & Behavior, 73, 145-158  https://doi.org/10.1016/S0031-9384(01)00472-3
 
Heilig Markus (2004): The NPY system in stress, anxiety and depression. Neuropeptides, 38, 213-224  https://doi.org/10.1016/j.npep.2004.05.002
 
Kanitz Ellen, Manteuffel Gerhard, Otten Winfried (1998): Effects of weaning and restraint stress on glucocorticoid receptor binding capacity in limbic areas of domestic pigs. Brain Research, 804, 311-315  https://doi.org/10.1016/S0006-8993(98)00712-4
 
Kormos Viktória, Gaszner Balázs (2013): Role of neuropeptides in anxiety, stress, and depression: From animals to humans. Neuropeptides, 47, 401-419  https://doi.org/10.1016/j.npep.2013.10.014
 
Lathe R (2001): Hormones and the hippocampus. Journal of Endocrinology, 169, 205-231  https://doi.org/10.1677/joe.0.1690205
 
Mei Hongyuan, Yang Binyu, Luo Juan, Gan Ling (2016): The effect of mixing levels on aggression at weaning in piglets. Applied Animal Behaviour Science, 179, 32-38  https://doi.org/10.1016/j.applanim.2016.03.009
 
Melotti Luca, Oostindjer Marije, Bolhuis J. Elizabeth, Held Suzanne, Mendl Michael (2011): Coping personality type and environmental enrichment affect aggression at weaning in pigs. Applied Animal Behaviour Science, 133, 144-153  https://doi.org/10.1016/j.applanim.2011.05.018
 
Morales-Medina Julio César, Dumont Yvan, Quirion Rémi (2010): A possible role of neuropeptide Y in depression and stress. Brain Research, 1314, 194-205  https://doi.org/10.1016/j.brainres.2009.09.077
 
National Pork Board (1996): Swine Care Handbook. Des Moines, USA.
 
National Research Council (2012): Nutrients Requirements of Swine. 11th Ed. National Academies Press, Washington DC., USA.
 
Painsipp Evelin, Herzog Herbert, Holzer Peter (2008): Implication of neuropeptide-Y Y2 receptors in the effects of immune stress on emotional, locomotor and social behavior of mice. Neuropharmacology, 55, 117-126  https://doi.org/10.1016/j.neuropharm.2008.05.004
 
Pu Shuye, Jain Mukul R., Horvath Tamas L., Diano Sabrina, Kalra Pushpa S., Kalra Satya P. (1999): Interactions between Neuropeptide Y and γ -Aminobutyric Acid in Stimulation of Feeding: A Morphological and Pharmacological Analysis 1. Endocrinology, 140, 933-940  https://doi.org/10.1210/endo.140.2.6495
 
Simmons P S, Miles J M, Gerich J E, Haymond M W (1984): Increased proteolysis. An effect of increases in plasma cortisol within the physiologic range.. Journal of Clinical Investigation, 73, 412-420  https://doi.org/10.1172/JCI111227
 
Stookey Joseph M., Gonyou Harold W. (1994): The effects of regrouping on behavioral and production parameters in finishing swine1. Journal of Animal Science, 72, 2804-2811  https://doi.org/10.2527/1994.72112804x
 
TAJALLI S, JONAIDI H, ABBASNEJAD M, DENBOW D (2006): Interaction between nociceptin/orphanin FQ (N/OFQ) and GABA in response to feeding. Physiology & Behavior, 89, 410-413  https://doi.org/10.1016/j.physbeh.2006.07.009
 
TUCHSCHERER M, KANITZ E, PUPPE B, TUCHSCHERER A, STABENOW B (2004): Effects of postnatal social isolation on hormonal and immune responses of pigs to an acute endotoxin challenge. Physiology & Behavior, 82, 503-511  https://doi.org/10.1016/j.physbeh.2004.04.056
 
Van Putten G., Dammers J. (1976): A comparative study of the well-being of piglets reared conventionally and in cages. Applied Animal Ethology, 2, 339-356  https://doi.org/10.1016/0304-3762(76)90067-5
 
Wang D.M., Wang C., Liu H.Y., Liu J.X., Ferguson James D. (2013): Effects of rumen-protected γ-aminobutyric acid on feed intake, lactation performance, and antioxidative status in early lactating dairy cows. Journal of Dairy Science, 96, 3222-3227  https://doi.org/10.3168/jds.2012-6285
 
XIE Wan-ying, HOU Xin-yan, YAN Feng-bin, SUN Gui-rong, HAN Rui-li, KANG Xiang-tao (2013): Effect of γ-aminobutyric acid on growth performance and immune function in chicks under beak trimming stress. Animal Science Journal, 84, 121-129  https://doi.org/10.1111/j.1740-0929.2012.01051.x
 
Yang Binyu, Mei Hongyuan, Zuo Fuyuan, Gan Ling (2017): Expression of microRNAs associated with oxidative stress in the hippocampus of piglets. Genes & Genomics, 39, 701-712  https://doi.org/10.1007/s13258-017-0537-4
 
Yang X.J., Pan C.L., Zhu Y.M., Huang Y.L., Zhang Y.S., Li A.X., Chen W.H., Zou S.X. (2009): Effects of aminobutyric acid on growth performance and hormone levels in serum of weaning piglets. Chinese Journal of Animal Nutrition, 21, 388–392. (in Chinese)
 
ZHANG Min, ZOU Xiao-ting, LI Hui, DONG Xin-yang, ZHAO Wenjing (2012): Effect of dietary γ-aminobutyric acid on laying performance, egg quality, immune activity and endocrine hormone in heat-stressed Roman hens. Animal Science Journal, 83, 141-147  https://doi.org/10.1111/j.1740-0929.2011.00939.x
 
supplementary materialdownload PDF

© 2019 Czech Academy of Agricultural Sciences