Interspecific hybridization of sturgeon species affects differently their gonadal developmentá Z., Havelka M., Pšenička M., Flajšhans M. (2018): Interspecific hybridization of sturgeon species affects differently their gonadal development. Czech J. Anim. Sci., 63: 1-10.
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Gonad development in fish is generally assumed to be negatively influenced by interspecific hybridization, resulting in sterility or sub-sterility. However, this is not the case in sturgeons (Acipenseridae), in which fertile hybrids are common. In the present study, we investigated gonad development in several sturgeon interspecific hybrids and purebred species. Six interspecific hybrid groups and three purebred groups were analyzed including 20 hybrid specimens with even ploidy, 40 specimens having odd ploidy levels, and 30 purebred specimens. Hybrids of species with the same ploidy (even ploidy – 2n, 4n) exhibited normally developed gonads similar to those seen in purebred specimens. In contrast, hybrids of species differing in ploidy (odd ploidy – 3n) did not display fully developed gonads. Ovaries were composed of oocytes or nests of differentiating oocytes that ceased development in early stages of meiosis (pachytene to zygotene) with a higher content of adipose and apoptotic tissue. Testes contained single spermatogonia along with Sertoli cells and spaces lacking germ cells. The obtained results showed that gonad development was influenced by genetic origin and ploidy of the sturgeon hybrids and were consistent with full fertility of hybrids with even ploidy. Sterility of females, but possibly limited fertility of males, is suggested for hybrids with odd ploidy.

Allard R.W. (ed.) (1999): Principles of Plant Breeding. John Wiley and Sons, New York, USA.
Arefyev V.A. (1997): Sturgeon hybrids: natural reality and practical prospects. Aquaculture Magazine, 23, 53–58.
Bartley D.M., Rana K., Immink A.J. (2001): The use of inter-specific hybrids in aquaculture and fisheries. Reviews in Fish Biology and Fisheries, 10, 325–337.
Bemis W.E., Findeis E.K., Grande L. (1997): An overview of Acipenseriformes. Environmental Biology of Fishes, 48, 25–71.
Billard R., Lecointre G. (2001): Biology and conservation of sturgeon and paddlefish. Reviews in Fish Biology and Fisheries, 10, 355–392.
Birstein V.J., Hanner R., DeSalle R. (1997): Phylogeny of the Acipenseriformes: cytogenetic and molecular approaches. Environmental Biology of Fishes, 48, 127–155.
Bronzi P., Rosenthal H., Gessner J. (2011): Global sturgeon aquaculture production: an overview. Journal of Applied Ichthyology, 27, 169-175
Bytyutskyy D., Srp J., Flajšhans M. (2012): Use of Feulgen image analysis densitometry to study the effect of genome size on nuclear size in polyploid sturgeons. Journal of Applied Ichthyology, 28, 704-708
Bytyutskyy Dmytro, Kholodnyy Vitaliy, Flajšhans Martin (2014): 3-D structure, volume, and DNA content of erythrocyte nuclei of polyploid fish. Cell Biology International, 38, 708-715
Dowling Thomas E., Secor and Carol L. (1997): THE ROLE OF HYBRIDIZATION AND INTROGRESSION IN THE DIVERSIFICATION OF ANIMALS. Annual Review of Ecology and Systematics, 28, 593-619
Fahy E., Martin S., Mulrooney M. (1988): Interactions of roach and bream in an Irish reservoir. Archiv für Hydrobiologie, 114, 291–309.
Flajshans M., Vajcova V. (2000): Odd ploidy levels in sturgeon suggest a backcross of interspecific hexaploid sturgeon hybrids to evolutionary tetraploid and/or octaploid parental species. Folia Zoologica, 49, 133–138.
Fontana F., Zane L., Pepe A., Congiu L. (2007): Polyploidy in Acipenseriformes: cytogenetic and molecular approaches. In: Pisano E., Ozouf-Costaz C., Foresti F., Kapoor B.G. (eds): Fish Cytogenetics. Science Publisher, Enfield, USA, 385–403.
Fopp-Bayat Dorota (2010): Meiotic gynogenesis revealed not homogametic female sex determination system in Siberian sturgeon (Acipenser baeri Brandt). Aquaculture, 305, 174-177
Haldane J. B. S. (1922): Sex ratio and unisexual sterility in hybrid animals. Journal of Genetics, 12, 101-109
Havelka M., Hulák M., Bailie D. A., Prodöhl P. A., Flajšhans M. (2013): Extensive genome duplications in sturgeons: new evidence from microsatellite data. Journal of Applied Ichthyology, 29, 704-708
Keyvanshokooh S., Gharaei A. (2010): A review of sex determination and searches for sex-specific markers in sturgeon. Aquaculture Research, 41, e1–e7.
King T.L., Lubinski B.A., Spidle A.P. (2001): Microsatellite DNA variation in Atlantic sturgeon (Acipenser oxyrinchus oxyrinchus) and cross-species amplification in the Acipenseridae. Conservation Genetics, 2, 103–119.
Kopiejewska W., Terlecki J., Chybowski L. (2003): Varied somatic growth and sex cell development in reciprocal hybrids of roach Rutilus rutilus (L.) and ide Leuciscus idus (L.). Archives of Polish Fisheries, 11, 33–44.
Legendre M., Teugels G. G., Cauty C., Jalabert B. (1992): A comparative study on morphology, growth rate and reproduction of Clarias gariepinus (Burchell, 1822), Heterobranchus longifilis Valenciennes, 1840, and their reciprocal hybrids (Pisces, Clariidae). Journal of Fish Biology, 40, 59-79
Lu X., Shapiro J. A., Ting C.-T., Li Y., Li C., Xu J., Huang H., Cheng Y.-J., Greenberg A. J., Li S.-H., Wu M.-L., Shen Y., Wu C.-I. (2010): Genome-wide misexpression of X-linked versus autosomal genes associated with hybrid male sterility. Genome Research, 20, 1097-1102
Ludwig Arne, Lippold Sebastian, Debus Lutz, Reinartz Ralf (2009): First evidence of hybridization between endangered sterlets (Acipenser ruthenus) and exotic Siberian sturgeons (Acipenser baerii) in the Danube River. Biological Invasions, 11, 753-760
May B, Krueger C C, Kincaid H L (1997): Genetic variation at microsatellite loci in sturgeon: primer sequence homology in Acipenser and Scaphirhynchus. Canadian Journal of Fisheries and Aquatic Sciences, 54, 1542-1547
McQuown Eve C., Sloss Brian L., Sheehan Robert J., Rodzen Jeff, Tranah Gregory J., May Bernie (2000): Microsatellite Analysis of Genetic Variation in Sturgeon: New Primer Sequences for Scaphirhynchus and Acipenser. Transactions of the American Fisheries Society, 129, 1380-1388<1380:MAOGVI>2.0.CO;2
Mugue N. S., Barmintseva A. E., Rastorguev S. M., Mugue V. N., Barmintsev V. A. (2008): Polymorphism of the mitochondrial DNA control region in eight sturgeon species and development of a system for DNA-based species identification. Russian Journal of Genetics, 44, 793-798
OMOTO NAOTAKA, MAEBAYASHI MAMORU, ADACHI SHINJI, ARAI KATSUTOSHI, YAMAUCHI KOUHEI (2002): Histological observations of gonadal development in gynogenetic diploids and triploids of a hybrid sturgeon, bester. Fisheries science, 68, 1271-1272
Rab P. (1986): A note on the karyotype of the sterlet, Acipenser ruthenus (Pisces, Acipenseridae). Folia Zoologica, 35, 73–78.
Rosati A., Tewolde A., Mosconi C. (eds) (2007): Animal Production and Animal Science Worldwide. Wageningen Academic Publishers, Wageningen, the Netherlands.
Rzepkowska Malgorzata, Ostaszewska Teresa, Gibala Monika, Roszko Marek Lukasz (2014): Intersex Gonad Differentiation in Cultured Russian (Acipenser gueldenstaedtii) and Siberian (Acipenser baerii) Sturgeon1. Biology of Reproduction, 90, -
Seehausen Ole (2004): Hybridization and adaptive radiation. Trends in Ecology & Evolution, 19, 198-207
Soltis Pamela S., Soltis Douglas E. (2009): The Role of Hybridization in Plant Speciation. Annual Review of Plant Biology, 60, 561-588
Vasil’ev V. P., Vasil’eva E. D., Shedko S. V., Novomodny G. V. (2010): How many times has polyploidization occurred during acipenserid evolution? New data on the karyotypes of sturgeons (Acipenseridae, Actinopterygii) from the Russian Far East. Journal of Ichthyology, 50, 950-959
Vasil′ev Victor (2014): Karyological study in backcross hybrids between the sterlet, Acipenser ruthenus, and kaluga, A. dauricus (Actinopterygii: Acipenseriformes: Acipenseridae): A. ruthenus × (A. ruthenus × A. dauricus) and A. dauricus × (A. ruthenus × A. dauricus). Acta Ichthyologica et Piscatoria, 44, 301-308
Żelazowska Monika, Fopp-Bayat Dorota (2017): Previtellogenic and vitellogenic oocytes in ovarian follicles of cultured siberian sturgeon Acipenser baerii (Chondrostei, Acipenseriformes). Journal of Morphology, 278, 50-61
Żelazowska M., Jankowska W., Plewniak E., Rajek U. (2015): Ovarian nests in cultured Russian sturgeon Acipenser gueldenstaedtii and North American paddlefish Polyodon spathula comprised of previtellogenic oocytes. Journal of Fish Biology, 86, 1669-1679
Zhang Xiaomin, Wu Wenhua, Li Linmiao, Ma Xufa, Chen Jinping (2013): Genetic variation and relationships of seven sturgeon species and ten interspecific hybrids. Genetics Selection Evolution, 45, 21-
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