Expression of ESR1, PRLR, GHR, and IGF1R in mammary glands of Hu sheep with four teats

https://doi.org/10.17221/3/2018-CJASCitation:Zhang L., Peng F., Yu F., Wan L., Zhou Z.-. (2019): Expression of ESR1, PRLR, GHR, and IGF1R in mammary glands of Hu sheep with four teats. Czech J. Anim. Sci., 64: 49-58.
download PDF

Supernumerary teats are commonly found in sheep. To investigate the expression levels of hormone receptors in supernumerary teats of Hu sheep, mammary tissue samples were collected from two groups of Hu sheep, four- and two-teat sheep, during adolescent (A), pregnancy (P), lactation (L), and non-pregnancy (N). Using hematoxylin and eosin staining, we found that mammary alveoli were denser in the four-teat sheep than in the two-teat sheep during P and L. Immunohistochemistry and Western blotting were used to detect the expression patterns and relative protein expression levels of the estrogen receptor 1 (ESR1), prolactin receptor (PRLR), growth hormone receptor (GHR), and insulin-like growth factor 1 receptor (IGF1R) in Hu sheep mammary glands during the four periods. All four receptors were mainly expressed in mammary epithelial cells and adipose cells. Furthermore, the expression levels of PRLR and GHR in the four-teat sheep were significantly higher than those in the two-teat sheep during P and L. Our data suggest that four-teat sheep have more developed mammary gland tissue compared with two-teat sheep.

References:
Binart Nadine, Imbert-Bolloré Prune, Baran Nathalie, Viglietta Céline, Kelly Paul A. (2003): A Short Form of the Prolactin (PRL) Receptor Is Able to Rescue Mammopoiesis in Heterozygous PRL Receptor Mice. Molecular Endocrinology, 17, 1066-1074  https://doi.org/10.1210/me.2002-0181
 
Brisken Cathrin, Rajaram Renuga Devi (2006): Alveolar and Lactogenic Differentiation. Journal of Mammary Gland Biology and Neoplasia, 11, 239-248  https://doi.org/10.1007/s10911-006-9026-0
 
Brka M., Reinsch N., Kalm E. (2002): Frequency and Heritability of Supernumerary Teats in German Simmental and German Brown Swiss Cows. Journal of Dairy Science, 85, 1881-1886  https://doi.org/10.3168/jds.S0022-0302(02)74262-8
 
Buckels A. (2013): IGF1 receptor contributes to GH signaling and influences GH-induced GHR downregulation in LNCaP human prostate cancer cells. Heart Lung and Circulation, 21, S160–S161.
 
Connor E E (2005): Chromosomal mapping and quantitative analysis of estrogen-related receptor alpha-1, estrogen receptors alpha and beta and progesterone receptor in the bovine mammary gland. Journal of Endocrinology, 185, 593-603  https://doi.org/10.1677/joe.1.06139
 
Dessauge F., Finot L., Wiart S., Aubry J.M., Ellis S.E. (2009): Effects of ovariectomy in prepubertal goats. Journal of Physiology and Pharmacology, 60 (Suppl. 3), 127–133.
 
Feng Y., Manka D., Wagner K.-U., Khan S. A. (2007): Estrogen receptor-  expression in the mammary epithelium is required for ductal and alveolar morphogenesis in mice. Proceedings of the National Academy of Sciences, 104, 14718-14723  https://doi.org/10.1073/pnas.0706933104
 
GOODMAN GORDON T., MICHAEL AKERS R., FRIDERICI KAREN H., ALLEN TUCKER H. (1983): Hormonal Regulation of α-Lactalbumin Secretion from Bovine Mammary Tissue Cultured in Vitro*. Endocrinology, 112, 1324-1330  https://doi.org/10.1210/endo-112-4-1324
 
Ilkbahar Y. (1999): Differential expression of the growth hormone receptor and growth hormone-binding protein in epithelia and stroma of the mouse mammary gland at various physiological stages. Journal of Endocrinology, 161, 77-87  https://doi.org/10.1677/joe.0.1610077
 
Kashyap D.K., Giri D.K., Dewangan G. (2014): Prevalence of udder and teat affections in non-descript goats in Rajasthan. Indian Journal of Small Ruminants, 20, 131–133.
 
Kaygisiz A. (2010): Gene frequencies and heritability of supernumerary teats and its relationship with milk yields of Holstein and Brown Swiss. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 16, 561–566.
 
Kelly Paul A, Bachelot Anne, Kedzia Cécile, Hennighausen Lothar, Ormandy Christopher J, Kopchick John J, Binart Nadine (2002): The role of prolactin and growth hormone in mammary gland development. Molecular and Cellular Endocrinology, 197, 127-131  https://doi.org/10.1016/S0303-7207(02)00286-1
 
Knight C.H., Peaker M. (1978): Development of the mammary gland. British Medical Journal, 65, 521–536.
 
Kotrulja L. (2015): Polythelia and associated hydronephrosis. Case Reports in Perinatal Medicine, 4, 149–150.
 
Lawrence T.L.J., Fowler V.R. (2002): Growth of Farm Animals. CABI Publishing, Wallingford, UK.
 
Loesch K.A. (2006): Mechanism and regulation of growth hormone receptor proteolysis and shedding. Dissertations and Theses, GradWorks.
 
Marshman Emma, Streuli Charles H (2002): Insulin-like growth factors and insulin-like growth factor binding proteins in mammary gland function. Breast Cancer Research, 4, -  https://doi.org/10.1186/bcr535
 
Mueller Stefan O., Clark James A., Myers Page H., Korach Kenneth S. (2002): Mammary Gland Development in Adult Mice Requires Epithelial and Stromal Estrogen Receptor α. Endocrinology, 143, 2357-2365  https://doi.org/10.1210/endo.143.6.8836
 
Oppong E., Gumedze J. (1981): Supernumerary teats in Ghanaian livestock. I. Sheep and goats. Beitrage zur tropischen Landwirtschaft und Veterinarmedizin, 20, 63–67.
 
Palacios Carlos, Abecia José-Alfonso (2014): Supernumerary Teat Removal Can Be Avoided in Dairy Sheep. Journal of Applied Animal Welfare Science, 17, 178-182  https://doi.org/10.1080/10888705.2014.884404
 
Pausch Hubert, Emmerling Reiner, Schwarzenbacher Hermann, Fries Ruedi (2016): A multi-trait meta-analysis with imputed sequence variants reveals twelve QTL for mammary gland morphology in Fleckvieh cattle. Genetics Selection Evolution, 48, -  https://doi.org/10.1186/s12711-016-0190-4
 
Peng W.-F., Xu S.-S., Ren X., Lv F.-H., Xie X.-L., Zhao Y.-X., Zhang M., Shen Z.-Q., Ren Y.-L., Gao L., Shen M., Kantanen J., Li M.-H. (2017): A genome-wide association study reveals candidate genes for the supernumerary nipple phenotype in sheep ( Ovis aries ). Animal Genetics, 48, 570-579  https://doi.org/10.1111/age.12575
 
Saunier Elise, Dif Fariel, Kelly Paul A., Edery Marc (2003): Targeted Expression of the Dominant-Negative Prolactin Receptor in the Mammary Gland of Transgenic Mice Results in Impaired Lactation. Endocrinology, 144, 2669-2675  https://doi.org/10.1210/en.2002-221038
 
Sejrsen K., Purup S., Vestergaard M., Weber M.S., Knight C.H. (1999): Growth hormone and mammary development. Domestic Animal Endocrinology, 17, 117-129  https://doi.org/10.1016/S0739-7240(99)00029-6
 
Silberstein Gary B. (2001): Postnatal mammary gland morphogenesis. Microscopy Research and Technique, 52, 155-162  https://doi.org/10.1002/1097-0029(20010115)52:2<155::AID-JEMT1001>3.0.CO;2-P
 
Walden Paul D., Ruan Weifeng, Feldman Mark, Kleinberg David L. (1998): Evidence That the Mammary Fat Pad Mediates the Action of Growth Hormone in Mammary Gland Development. Endocrinology, 139, 659-662  https://doi.org/10.1210/endo.139.2.5718
 
Wallace C. (1953): Observations on mammary development in calves and lambs. The Journal of Agricultural Science, 43, 413-  https://doi.org/10.1017/S0021859600057890
 
download PDF

© 2019 Czech Academy of Agricultural Sciences