Gender and age effects on the expression of genes related to lipid metabolism in broiler’s liver

 

https://doi.org/10.17221/41/2017-CJASCitation:de Souza Khatlab A., Del Vesco A.P., Gasparino E., de Oliveira Neto A.R. (2018): Gender and age effects on the expression of genes related to lipid metabolism in broiler’s liver  . Czech J. Anim. Sci., 63: 103-109.
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Two experiments were conducted to assess gender (Experiment 1) and age (Experiment 2) effects on the expression of genes related to lipid metabolism in broiler chickens. The expression of fatty acid synthase (FAS), apolipoprotein A-I (APOA-I), apolipoprotein B (APOB), adiponectin (ADIPOQ), liver kinase B1 (LKB1), and AMP-activated protein kinase α-1 (AMPKα-1) genes was evaluated by qRT-PCR. In Experiment 1, we observed a gender effect on feed intake, as male broilers presented greater feed intake than females. Female broilers presented greater gene expression of FAS, and lower expression of ADIPOQ and AMPKα-1, than males. A gender effect was not observed for the gene expression of APOA-I, APOB, or LKB1. In Experiment 2, there was a significant age effect on feed intake and weight gain. Broilers 42 days of age presented greater feed intake and weight gain than 21-day-old birds. 21-day-old broilers showed greater expression of APOA-I, ADIPOQ, LKB1, and AMPKα-1, and lower APOB gene expression in the liver than 42-day-old broilers. Age had no effect on FAS gene expression. Our results show that the gender and age could act on the expression of genes related to lipid synthesis, such as FAS and APOB, and also on genes related to lipid oxidation, such as ADIPOQ, LKB1, and AMPK.

References:
Berg J.M., Tymoczko J.L., Stryer L. (eds) (2012): Biochemistry. Guanabara Koogan, Rio de Janeiro, Brazil. (in Portuguese)
 
Cobb-Vantress (2016): COBB Breeder Management Guide. Available at http://www.cobb-vantress.com/docs/default-source/management-guides/cobb-breeder-management-guide---english.pdf (accessed Feb 1, 2017)
 
Cruz Valdecy Aparecida Rocha da, Schenkel Flávio Schramm, Savegnago Rodrigo Pelicioni, Grupioni Natalia Vinhal, Stafuzza Nedenia Bonvino, Sargolzaei Mehdi, Ibelli Adriana Mércia Guaratini, Peixoto Jane de Oliveira, Ledur Mônica Corrêa, Munari Danísio Prado, Davoli Roberta (2015): Association of Apolipoprotein B and Adiponectin Receptor 1 Genes with Carcass, Bone Integrity and Performance Traits in a Paternal Broiler Line. PLOS ONE, 10, e0136824-  https://doi.org/10.1371/journal.pone.0136824
 
Gaya L.G., Mourao G.B., Ferraz J.B.S. (2006): Genetic-quantitative aspects of performance, carcass and body composition traits in broilers. Ciência Rural, 36, S709–S716. (in Portuguese)
 
Ginsberg H. N. (): New Perspectives on Atherogenesis: Role of Abnormal Triglyceride-Rich Lipoprotein Metabolism. Circulation, 106, 2137-2142  https://doi.org/10.1161/01.CIR.0000035280.64322.31
 
Hawley S.A., Boudeau J., Reid J.L., Mustard K.J., Udd L., Makela T.P., Alessi D.R., Hardie D.G. (2003): Complexes between the LKB1 tumor suppressor, STRADα/β and MO25α/β are upstream kinases in the AMP-activated protein kinase cascade. Journal of Biology, 2, 28. https://doi.org/10.1186/1475-4924-2-28
 
Hermier D. (1997): Lipoprotein metabolism and fattening in poultry. The Journal of Nutrition, 127, 805S–808S.
 
Jiang R. R., Zhao G. P., Zhao J. P., Chen J. L., Zheng M. Q., Liu R. R., Wen J. (2014): Influence of dietary nicotinic acid supplementation on lipid metabolism and related gene expression in two distinct broiler breeds of female chickens. Journal of Animal Physiology and Animal Nutrition, 98, 822-829  https://doi.org/10.1111/jpn.12138
 
Liu Lei, Zhu Lixian (2012): Effect of 24 h Fasting on Gene Expression of AMPK, Appetite Regulation Peptides and Lipometabolism Related Factors in the Hypothalamus of Broiler Chicks. Asian-Australasian Journal of Animal Sciences, 25, 1300-1308  https://doi.org/10.5713/ajas.2012.12153
 
Livak Kenneth J., Schmittgen Thomas D. (2001): Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method. Methods, 25, 402-408  https://doi.org/10.1006/meth.2001.1262
 
Mildner Ana M., Clarke Steven D. (1991): Porcine Fatty Acid Synthase: Cloning of a Complementary DNA, Tissue Distribution of Its mRNA and Suppression of Expression by Somatotropin and Dietary Protein. The Journal of Nutrition, 121, 900-907  https://doi.org/10.1093/jn/121.6.900
 
Musundire M. T., Halimani T. E., Chimonyo M. (2017): Physical and chemical properties of meat from scavenging chickens and helmeted guinea fowls in response to age and sex. British Poultry Science, 58, 390-396  https://doi.org/10.1080/00071668.2017.1313961
 
Nelson D.L., Cox M.M. (eds) (2011): Lehninger Principles of Biochemistry. Worth Publishers, New York, USA.
 
Nogalska Anna, Swierczynski Julian (2001): The age-related differences in obese and fatty acid synthase gene expression in white adipose tissue of rat. Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, 1533, 73-80  https://doi.org/10.1016/S1388-1981(01)00142-1
 
Novak E.M., Bydlowski S.P. (1996): Molecular biology of dyslipidemias. Genetic variation of apolipoproteins. Arquivos Brasileiros de Cardiologia, 67, 411–417. (in Portuguese)
 
Rostagno H.S., Albino L.F.T., Donzele J.L., Gomes P.C., Oliveira R.F., Lopes D.C., Ferreira A.S., Barreto S.L.T., Euclides R.F. (eds) (2011): Brazilian Tables for Birds and Pigs: Composition of Foods and Nutritional Requirements. Federal University of Viçosa, Viçosa, Brazil.
 
Saadoun Ali, Leclercq Bernard (1987): In Vivo Lipogenesis of Genetically Lean and Fat Chickens: Effects of Nutritional State and Dietary Fat. The Journal of Nutrition, 117, 428-435  https://doi.org/10.1093/jn/117.3.428
 
Shaw R. J. (2005): The Kinase LKB1 Mediates Glucose Homeostasis in Liver and Therapeutic Effects of Metformin. Science, 310, 1642-1646  https://doi.org/10.1126/science.1120781
 
Silva C.R. (2012): Performance and deposition of nutrients of broiler chickens fed with different lysine levels. Ph.D. Thesis. Minas Gerais, Brasil: Federal University of Viçosa. (in Portuguese)
 
Smink W., Gerrits W. J. J., Hovenier R., Geelen M. J. H., Verstegen M. W. A., Beynen A. C. (2010): Effect of dietary fat sources on fatty acid deposition and lipid metabolism in broiler chickens. Poultry Science, 89, 2432-2440  https://doi.org/10.3382/ps.2010-00665
 
Spady D. K. (1999): Reverse Cholesterol Transport and Atherosclerosis Regression. Circulation, 100, 576-578  https://doi.org/10.1161/01.CIR.100.6.576
 
Tumova E., Teimouri A. (2010): Fat deposition in the broiler chicken: a review. Scientia Agriculturae Bohemica, 41, 121–128.
 
Wang Yu, Zhou Mingyan, Lam Karen S. L., Xu Aimin (2009): Protective roles of adiponectin in obesity-related fatty liver diseases: mechanisms and therapeutic implications. Arquivos Brasileiros de Endocrinologia & Metabologia, 53, 201-212  https://doi.org/10.1590/S0004-27302009000200012
 
Whitehead J. P., Richards A. A., Hickman I. J., Macdonald G. A., Prins J. B. (2006): Adiponectin - a key adipokine in the metabolic syndrome. Diabetes, Obesity and Metabolism, 8, 264-280  https://doi.org/10.1111/j.1463-1326.2005.00510.x
 
Winder W. W., Hardie D. G. (1999): AMP-activated protein kinase, a metabolic master switch: possible roles in Type 2 diabetes. American Journal of Physiology-Endocrinology and Metabolism, 277, E1-E10  https://doi.org/10.1152/ajpendo.1999.277.1.E1
 
Wu G. Q., Deng X. M., Li J. Y., Li N., Yang N. (2006): A Potential Molecular Marker for Selection Against Abdominal Fatness in Chickens. Poultry Science, 85, 1896-1899  https://doi.org/10.1093/ps/85.11.1896
 
Yamauchi Toshimasa, Kamon Junji, Ito Yusuke, Tsuchida Atsushi, Yokomizo Takehiko, Kita Shunbun, Sugiyama Takuya, Miyagishi Makoto, Hara Kazuo, Tsunoda Masaki, Murakami Koji, Ohteki Toshiaki, Uchida Shoko, Takekawa Sato, Waki Hironori, Tsuno Nelson H., Shibata Yoichi, Terauchi Yasuo, Froguel Philippe, Tobe Kazuyuki, Koyasu Shigeo, Taira Kazunari, Kitamura Toshio, Shimizu Takao, Nagai Ryozo, Kadowaki Takashi (2003): Cloning of adiponectin receptors that mediate antidiabetic metabolic effects. Nature, 423, 762-769  https://doi.org/10.1038/nature01705
 
Zerehdaran S., Vereijken A. L. j., Arendonk J. A. M., van der Waaij E. H. (2005): Effect of age and housing system on genetic parameters for broiler carcass traits. Poultry Science, 84, 833-838  https://doi.org/10.1093/ps/84.6.833
 
Zhang S., Li H., Shi H. (2006): Single Marker and Haplotype Analysis of the Chicken Apolipoprotein B Gene T123G and D9500D9- Polymorphism Reveals Association with Body Growth and Obesity. Poultry Science, 85, 178-184  https://doi.org/10.1093/ps/85.2.178
 
Zhou Gaochao, Myers Robert, Li Ying, Chen Yuli, Shen Xiaolan, Fenyk-Melody Judy, Wu Margaret, Ventre John, Doebber Thomas, Fujii Nobuharu, Musi Nicolas, Hirshman Michael F., Goodyear Laurie J., Moller David E. (2001): Role of AMP-activated protein kinase in mechanism of metformin action. Journal of Clinical Investigation, 108, 1167-1174  https://doi.org/10.1172/JCI13505
 
Zhuo Zhu, Lamont Susan J., Lee William R., Abasht Behnam, Brockmann Gudrun A. (2015): RNA-Seq Analysis of Abdominal Fat Reveals Differences between Modern Commercial Broiler Chickens with High and Low Feed Efficiencies. PLOS ONE, 10, e0135810-  https://doi.org/10.1371/journal.pone.0135810
 
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