Association of missense MTTP gene polymorphism with carcass characteristics and meat quality traits in pigs

https://doi.org/10.17221/45/2015-CJASCitation:Ropka-Molik K., Podstawski P., Piórkowska K., Tyra M. (2017): Association of missense MTTP gene polymorphism with carcass characteristics and meat quality traits in pigs. Czech J. Anim. Sci., 62: 9-14.
supplementary materialdownload PDF
Microsomal triglyceride transfer protein, coded by MTTP gene, has multiple functions including participation in formation of chylomicrons, low-density lipoproteins, and very low-density lipoproteins. Therefore MTTP protein plays a key role in the transport of fats and cholesterol between membrane vesicles, which can be associated with lipid metabolism. In the present study, ENSSSCT00000010052.2:c.2518C>T (rs335896411) missense polymorphism (Leu>Phe) located in exon 18 of MTTP gene was investigated in order to estimate its potential association with production traits of pigs. The analysis was performed with five breeds (Duroc, Landrace, Large White, Pietrain, Pulawska pigs) and totally 678 pigs, for which the genotypes of c.2518C>T polymorphism were identified by the polymerase chain reaction–restriction fragment length polymorphism method. The present study showed a significant association of c.2518C>T polymorphism with carcass yield. When analyzing the whole population, CC homozygotes showed significantly higher carcass yield than heterozygotes (P ≤ 0.05). Moreover, c.2518C>T single nucleotide polymorphism (SNP) affected pH measured in loin (m. longissimus dorsi) and ham (m. semimembranosus) 45 min after slaughter. For both parameters, the highest pH values were obtained for CC pigs, while the lowest for heterozygotes (P ≤ 0.05). The SNP analyzed was also related with meat colour (yellowness intensity (b*)). Previous research confirmed that ENSSSCP00000009789.2:p.Leu840Phe polymorphism, via affecting MTTP protein activity, influences metabolism of fatty acids. Additionally, results obtained in the present study suggest that the analyzed missense mutation in porcine MTTP gene can be one of the potential genetic factors associated with meat quality (pork pH and colour) and carcass yield.
References:
Ai H., Ren J., Zhang Z., Ma J., Guo Y., Yang B., Huang L. (2012): Detection of quantitative trait loci for growth- and fatness-related traits in a large-scale White Duroc × Erhualian intercross pig population. Animal Genetics, 43, 383-391  https://doi.org/10.1111/j.1365-2052.2011.02282.x
 
Brozovic Suzana, Nagaishi Takashi, Yoshida Masaru, Betz Stephanie, Salas Azucena, Chen Daohong, Kaser Arthur, Glickman Jonathan, Kuo Timothy, Little Alicia, Morrison Jamin, Corazza Nadia, Kim Jin Yong, Colgan Sean P, Young Stephen G, Exley Mark, Blumberg Richard S (): CD1d function is regulated by microsomal triglyceride transfer protein. Nature Medicine, 10, 535-539  https://doi.org/10.1038/nm1043
 
Court M.H., Michael H. (2012): Court’s (2005−2008) online calculator. Available at: www.tufts.edu (accessed Jan 9, 2017).
 
Di Filippo M., Crehalet H., Samson-Bouma M. E., Bonnet V., Aggerbeck L. P., Rabes J.-P., Gottrand F., Luc G., Bozon D., Sassolas A. (): Molecular and functional analysis of two new MTTP gene mutations in an atypical case of abetalipoproteinemia. The Journal of Lipid Research, 53, 548-555  https://doi.org/10.1194/jlr.M020024
 
di Giuseppe Romina, Pechlivanis Sonali, Fisher Eva, Arregui Maria, Weikert Beate, Knüppel Sven, Buijsse Brian, Fritsche Andreas, Willich Stefan N, Joost Hans-Georg, Boeing Heiner, Moebus Susanne, Weikert Cornelia (2013): Microsomal triglyceride transfer protein -164 T > C gene polymorphism and risk of cardiovascular disease: results from the EPIC-Potsdam case-cohort study. BMC Medical Genetics, 14, 19-  https://doi.org/10.1186/1471-2350-14-19
 
Estelle J., Sanchez A., Folch J. M. (2005): Assignment of the microsomal triglyceride transfer protein large subunit (MTP ) gene to porcine chromosome 8. Animal Genetics, 36, 354-355  https://doi.org/10.1111/j.1365-2052.2005.01303.x
 
Estell�� J., Fern��ndez A. I., P��rez-Enciso M., Fern��ndez A., Rodr��guez C., S��nchez A., Noguera J. L., Folch J. M. (2009): A non-synonymous mutation in a conserved site of the MTTP gene is strongly associated with protein activity and fatty acid profile in pigs. Animal Genetics, 40, 813-820  https://doi.org/10.1111/j.1365-2052.2009.01922.x
 
Hussain Mohammed Mahmood (): Microsomal triglyceride transfer protein: a multifunctional protein. Frontiers in Bioscience, 8, s500-  https://doi.org/10.2741/1071
 
Hussain M Mahmood, Rava Paul, Walsh Meghan, Rana Muhammad, Iqbal Jahangir (2012): Multiple functions of microsomal triglyceride transfer protein. Nutrition & Metabolism, 9, 14-  https://doi.org/10.1186/1743-7075-9-14
 
Leung G. K. (): A Deficiency of Microsomal Triglyceride Transfer Protein Reduces Apolipoprotein B Secretion. Journal of Biological Chemistry, 275, 7515-7520  https://doi.org/10.1074/jbc.275.11.7515
 
Liu G., Jennen D. G. J., Tholen E., Juengst H., Kleinwächter T., Hölker M., Tesfaye D., Ün G., Schreinemachers H.-J., Murani E., Ponsuksili S., Kim J.-J., Schellander K., Wimmers K. (2007): A genome scan reveals QTL for growth, fatness, leanness and meat quality in a Duroc-Pietrain resource population. Animal Genetics, 38, 241-252  https://doi.org/10.1111/j.1365-2052.2007.01592.x
 
Oczkowicz Maria, Tyra Mirosław, Ropka-Molik Katarzyna, Mucha Aurelia, Żukowski Kacper (2012): Effect of IGF2 intron3-g.3072G>A on intramuscular fat (IMF) content in pigs raised in Poland. Livestock Science, 149, 301-304  https://doi.org/10.1016/j.livsci.2012.06.021
 
Pons Véronique, Rolland Corinne, Nauze Michel, Danjoux Marie, Gaibelet Gérald, Durandy Anne, Sassolas Agnès, Lévy Emile, Tercé François, Collet Xavier, Mas Emmanuel (2011): A severe form of abetalipoproteinemia caused by new splicing mutations of microsomal triglyceride transfer protein (MTTP). Human Mutation, 32, 751-759  https://doi.org/10.1002/humu.21494
 
Raabe M., Flynn L. M., Zlot C. H., Wong J. S., Veniant M. M., Hamilton R. L., Young S. G. (1998): Knockout of the abetalipoproteinemia gene in mice: Reduced lipoprotein secretion in heterozygotes and embryonic lethality in homozygotes. Proceedings of the National Academy of Sciences, 95, 8686-8691  https://doi.org/10.1073/pnas.95.15.8686
 
Ropka-Molik K., Dusik A., Piórkowska K., Tyra M., Oczkowicz M., Szmatoła T. (2015): Polymorphisms of the membrane-associated ring finger 4, ubiquitin protein ligase gene (MARCH4) and its relationship with porcine production traits. Livestock Science, 178, 18-26  https://doi.org/10.1016/j.livsci.2015.05.022
 
Zampino R., Ingrosso D., Durante-Mangoni E., Capasso R., Tripodi M.-F., Restivo L., Zappia V., Ruggiero G., Adinolfi L. E. (2008): Microsomal triglyceride transfer protein ( MTP ) -493G/T gene polymorphism contributes to fat liver accumulation in HCV genotype 3 infected patients. Journal of Viral Hepatitis, 15, 740-746  https://doi.org/10.1111/j.1365-2893.2008.00994.x
 
supplementary materialdownload PDF

© 2021 Czech Academy of Agricultural Sciences | Prohlášení o přístupnosti