Effects of enriched mesquite piperidine alkaloid extract in diets with reduced crude protein concentration on the rumen microbial efficiency and performance in lambs

https://doi.org/10.17221/54/2020-CJASCitation:Ferreira Brito E., Albuquerque Pereira M.L., de Oliveira Silva H.G., Maia Soares A.C., Soares Correia G., Borges Sousa L., Borges Sousa L., dos Santos Soares V.P., Fonseca de Paula V. (2020): Effects of enriched mesquite piperidine alkaloid extract in diets with reduced crude protein concentration on the rumen microbial efficiency and performance in lambs. Czech J. Anim. Sci., 65: 268-280.
download PDF

The objective of this study was to evaluate the effects of enriched mesquite piperidine alkaloid (MPA) extract at 31 mg/kg of dry matter (DM) in diets with 16% or 13% of crude protein (CP) compared to a diet with 16% CP without additive (control) and diets with monensin (MON) at 31 mg/kg DM and 16% or 13% CP. The intake, ingestive behaviour, apparent digestibility, body weight gain, microbial protein synthesis and nitrogen balance were evaluated. A total of 30 uncastrated crossbred Santa Inês × Bergamasca lambs were used and allocated in a completely randomized design. No effects of additives on dry matter (DM), organic matter (OM), neutral detergent fibre (NDF), and non-fibre carbohydrate (NFC) intake were observed in diets with 16% and 13% CP compared to the control diet. The total digestible nutrient intake (g/kg BW) for the diet with MPA 13% CP was lower than for the control diet, while the metabolisable energy intake (MJ/kg BW) did not differ between diets. The DM feeding rate (g/min) was reduced for the diet with MON 13% CP compared to the control diet. There was no difference between MON or MPA with CP 16% or 13% and the control diet in the digestibility of DM, OM, NFC, and NDF. MON in the diet with 13% CP reduced the BW gain, which differed from the control diet, while MPA 13% CP did not differ from the control diet. The microbial protein synthesis efficiency was higher for diets with MPA compared to MON 13% CP and the control diet. Dietary nitrogen retention (g/kg metabolic weight) was lower in diets with 13% CP that differed from the diets with 16% CP. The nitrogen retained as a percentage of ingested and digested nitrogen was unchanged with the use of MPA or MON 13% CP due to lower urinary nitrogen excretion. The MPA 13% CP diet does not affect the performance of lambs by increasing the microbial synthesis efficiency in the rumen.

References:
Alves EM, Pedreira MS, Oliveira CAS. Ingestive behavior of sheep fed mesquite pod meal associated with urea levels. Acta Sci Anim Sci. 2010 Oct;32(4):439-45.
 
Appuhamy JADRN, Strathe AB, Jayasundara S, Wagner-Riddle C, Dijkstra J, France J, Kebreab E. Anti-methanogenic effects of monensin in dairy and beef cattle: A meta-analysis. J Dairy Sci. 2013 Aug 1;96(8):5161-73. https://doi.org/10.3168/jds.2012-5923
 
Berchielli TT, Pires AV, Oliveira SG. Ruminant nutrition. 2nd ed. Jaboticabal: Funep; 2011.
 
Burger PJ, Pereira JC, Queiroz AC, Silva JFC, Valadares Filho SC, Cecon PR, Casali ADP. Ingestive behavior in Holstein calves fed diets containing different concentrate levels. Rev Bras Zootec. 2000 Jan;29(1):236-42. https://doi.org/10.1590/S1516-35982000000100031
 
Chen XB, Gomes MJ. Estimation of microbial protein supply to sheep and cattle based on urinary excretion of purine derivatives an overview of technical details. Bucksburnd: Rowett Research Institute; 1992. 21 p.
 
Chen XB, Chen YK, Franklin MF, Orskov ER, Shand WJ. The effect of feed intake and body weight on purine derivative excretion and microbial protein supply in sheep. J Anim Sci. 1992 May 1;70(5):1534-42. https://doi.org/10.2527/1992.7051534x
 
Detmann D, Valadares Filho SC. On the estimation of non-fibrous carbohydrates in feeds and diets. Arq Bras Med Vet Zootec. 2010 Aug;62(4):980-4. https://doi.org/10.1590/S0102-09352010000400030
 
Detmann E, Souza MA, Valadares Filho SC, Queiroz AC, Berchielli TT, Saliba EOS, Cabral LS, Pina DS, Ladeira MM, Azevedo JAG. Methods for food analysis. National Institute of Science and Technology of Animal Science. Viscount of Rio Branco: Suprema; 2012. 214 p.
 
Hall MB, Hoover WH, Jennings JP, Webster TKM. A method for partitioning neutral detergent-soluble carbohydrates. J Sci Food Agr. 1999 Dec;79(15):2079-86. https://doi.org/10.1002/(SICI)1097-0010(199912)79:15<2079::AID-JSFA502>3.0.CO;2-Z
 
Javed SI, Asif J, Muhammad A. Nutrient digestibility and feedlot performance of lambs fed diets variying protein and energy content. Trop Anim Health Pro. 2010 Jun 1;42(5):941-6. https://doi.org/10.1007/s11250-009-9511-8
 
Licitra G, Hernandez TM, Van Soest PJ. Standardization of procedures for nitrogen fracionation of ruminant feed. Anim Feed Sci Technol. 1996 Mar 1;57(4):347-58. https://doi.org/10.1016/0377-8401(95)00837-3
 
Mertens DR. Creating a system for meeting the fiber requirements of dairy cows. J Dairy Sci. 1997 Jul;80(7):1463-81. https://doi.org/10.3168/jds.S0022-0302(97)76075-2
 
Mertens DR. Gravimetric determination of amylase-treated neutral detergent fiber in feeds with refluxing in beaker or crucibles: Collaborative study. J AOAC Int. 2002 Nov 1;85(6):1217-40.
 
Miranda LF, Queiroz AC, Valadares Filho SC, Cecon PR, Pereira ES, Campos JMS, Lanna RP, Miranda JR. Ingestive behavior of dairy heifers fed sugarcane diets. Rev Bras Zootec. 1999 May 1;28(3):614-20. https://doi.org/10.1590/S1516-35981999000300026
 
NRC – National Research Council. Nutrient requirements of dairy cattle. 7th ed. Washington, DC, USA: National Academies Press; 2001. 408 p.
 
Ott-Longoni R, Viswanathan N, Hesse M. Die Konstitution des Alkaloides Juliprosopin aus Prosopis juliflora A. Dc. 176. Mitteilung über organische Naturstoffe A. Dc. [The structure of the alkaloide juliprosopine from Prosopis juliflora A. Dc.]. Helv Chim Acta. 1980;63:2119-29. German. https://doi.org/10.1002/hlca.19800630738
 
Pereira MLA, Batista R, inventors. Aditivo à base de extrato alcaloídico de vagens de algaroba (prosopis juliflora) em rações, utilizado como modificador da fermentação ruminal para melhoria do desempenho animal e mitigação da emissão de gases entéricos de efeito estufa [Additive based on plant extract used as a ruminal fermentation modifier to improve animal performance and mitigate greenhouse gas emissions]. Universidade Estadual do Sudoeste da Bahia-UESB, BR 10 2012 030155-5. 2014 Dec 30. Portuguese.
 
Pereira TCJ, Pereira MLA, Moreira JV, Azevedo JAG, Batista R, Paula VF, Oliveira BS, Santos EJS. Effects of alkaloid extracts of mesquite pod on the products of in vitro rumen fermentation. Environ Sci Pollut Res Int. 2017 Mar;24(5):4301-11. https://doi.org/10.1007/s11356-016-7761-3
 
Russell JB, Martin SA. Effects of various methane inhibitors on the fermentation of amino acids by mixed rumen micorganisms in vitro. J Anim Sci. 1984 Nov 1;59(5):1329-38. https://doi.org/10.2527/jas1984.5951329x
 
Salinas-Chavira J, Lara-Juarez A, Gil-Gonzalez A, Jimenez-Castro J, Garcia-Castillo R, Ramirez-Bribiesca E. Effect of breed type and ionophore supplementation on growth and carcass characteristic in feedlot hair lambs. Rev Bras Zootec. 2010 Mar;39(3):633-7. https://doi.org/10.1590/S1516-35982010000300024
 
Santos JRA. Extrato alcaloídico da farinha de vagens integrais de algarobeiras em dietas para cordeiros confinados [Alkaloid extract from the flours of whole pods of algarobeiras in diets for confined lambs] [dissertation]. Brasil: State University of Southwest Bahia; 2017. 77 p. Portuguese.
 
Santos ET, Pereira MLA, Silva CFPG, Souza-Neta LC, Geris R, Martins D, Santana AEG, Barbosa LCA, Silva HGO, Freitas GC, Figueiredo MP, Oliveira FF, Batista R. Antibacterial activity of the alkaloid-enriched extract from Prosopis juliflora pods and its influence on in vitro ruminal digestion. Int J Mol Sci. 2013 Apr;14(4):8496-516. https://doi.org/10.3390/ijms14048496
 
Sousa LB. Alcaloides piperidínicos de Prosopis juliflora como um aditivo nutricional para cordeiros [Piperidine alkaloids from Prosopis juliflora as a nutritional additive for lambs] [dissertation]. Brasil: State University of Southwest Bahia; 2018. Portuguese.
 
Weiss W. Energy prediction equations for ruminant feeds. Proceedings of the 61st Cornell Nutrition Conference for Feed Manufacturers Ithaca. Cornell University: New York; 1999. p. 176-85.
 
download PDF

© 2020 Czech Academy of Agricultural Sciences